WO2016159547A1 - Novel composition for pine wilt disease prevention containing aggregation pheromone 2-(dodecyloxy)ethanol isolated and purified from korean monochamus alternatus and method for preparing same - Google Patents

Abstract

The present invention relates to a composition for pine wilt disease prevention to attract and capture Monochamus alternatus and/or Monochamus saltuarius and a method for preparing the same, an agent for attracting and capturing Monochamus alternatus and/or Monochamus saltuarius to prevent pine wilt disease, the agent containing the composition adsorbed on a polyacrylic acid-based super absorbent resin, and a method for biological prevention of pine wilt disease to attract and capture Monochamus alternatus and/or Monochamus saltuarius. According to the present invention, 2-(dodecyloxy)ethanol, which is an aggregation pheromone isolated and purified from Korean Monochamus alternatus, is especially noteworthy in that it has a remarkable effect in preventing pine wilt disease by being used alone or together with ethanol/alpha-pinene to attract and capture Monochamus alternatus and Monochamus saltuarius and it can fundamentally prevent pine wilt disease spreading insects by an eco-friendly biological method.

Description

Novel Pineapple Nematode Disease Control Composition and Method for Containing Collected Pheromone 2- (dodecyloxy) ethanol Isolated and Purified from Korean Saltwater Bovine Cattle

The present invention is a pine nematode disease control composition and method for producing pineapple nematode disease attracting and capturing monochamus alternatus and / or Monochamus saltuarius, the composition is adsorbed on a super absorbent resin pine pine nematode disease control The present invention relates to a method for biological control of pineal nematode disease, which attracts and captures a saltwater cow and / or a northern saltwater cow, and a saltwater cow and / or a northern saltwater cow.

Pine wilt disease is known as Bursaphelenchus xylophilus) is a disease that causes the tree to wither due to wilting symptoms by disturbing the movement of moisture and nutrients in pine trees (Futai K., Pine wood nematode, Bursaphelenchus xylophilus. Annu. Rev. Phytopathol) , 51: 61-83, 2013). The damage of pineal nematode diseases has been reported in many parts of the world, including the United States, Europe, and Asia. In Korea, the pineal nematode disease has spread nationwide since 2011, causing not only environmental problems but also enormous economic losses.

Pine reptiles are spread by the mediator Monochamus alternatus and / or Monochamus saltuarius because they do not have the ability to move themselves to other trees, and most infected pines die completely within three months of infection and the treatment is No preventive action is very important.

Currently, the control of pine ash nematode disease is being carried out by fumigation control method of harvesting dead trees and fumigation, physical control method of incineration and shredding after logging, and chemical control method of injecting or air-spraying preventives into trees (Tan).et al., A Study on disease development and early diagnosis of pine wood nematode, Bursaphelenchus xylophilus, infection of japanese black pine, Nematology 7: 481-485, 2005). However, the above methods have a problem that it consumes a lot of manpower and a huge budget, and is not a method of controlling the spread of damage through the control of the mediated insects of the pine tree nematode.

Recently, bark beetle chiromon ipsdienol and ipsenol, which are information on insect livers, have been attracted to the European Monoceros galloprovincialis, while the Chinese Monoceros alternatus has been reported to have no attracting effect. Fan et al., Effect of bark beetle pheromones on the attraction of Monochamus alternatus to pine volatiles, Insect Sci., 17: 553-556, 2010). In addition, 2- (undecyloxy) ethanol, an aggregated pheromone of European sea salt, has been reported (JA Pajares et al., Identification and field activity of a male-produced aggregation pheromone in the pine sawyer beetle, Monochamus galloprovincialis, J. Chem Ecol, 36: 570-583, 2010; SA Teale et al, a male-produced aggregation pheromone of Monochamus alternatus... (Coleoptera: Cerambycidae), a major vector of pine wood nematode, J. Econ . Entomol . , 104: 1592-1598, 2011; JE Macias-Saman et al., 2-Undecyloxy-1-ethanol in combination with other semiochemicals attracts three Monochamus species (Coleoptera: Cerambycidae) in British Columbia, Canada, Can Entomol, 144:... ... 764-768, 2012; JA Pajares et al, 2- (Undecyloxy) -ethanol is a major component of the male-produced aggregation pheromone of Monochamus sutor, Entomol Exp Appl, 149: 118-127, 2013), the aggregated pheromone secreted by Korean sea salt beetles has not been reported. Development of the brush whiskers long-horned beetle trapping the development and Bursaphelenchus xylophilus control techniques using romon situation is nonexistent.

Superabsorbent resin is a resin that has the ability to absorb water from tens to thousands of times its own weight and was discovered in 1980 in corn utilization studies in the United States. The super absorbent polymer has a high absorption capacity and the resin itself sucks water, so it does not discharge water under any pressure. In the past, many fibers such as cotton wool and cotton, which absorb water by capillary action, have been used for water absorption research, but in recent years, products have been replaced by super absorbent polymers. The superabsorbent polymer is made by making a polymer backbone with carbon atoms and combining hydrophilic ion molecules. When the absorbency of water decreases, the hydrophilic ions bound to the intersected network of carbon chains in the superabsorbent polymer absorb water. Water can be trapped inside the superabsorbent polymer (GC Cha et al., Study on the Characteristics of the Absorbency Silicone by Super Absorbent Polymers, Elastomers and Composites Vol. 47, No. 2, pp. 141 ~ 147, 2012) .

As a prior art of the present invention, a pine nematode attractant composition containing a camphor as an active ingredient, and a kit for assaying pine nematode diseases using the same are known from the Republic of Korea Patent Publication No. 10-1032301, which is a camphor as an active ingredient The present invention relates to a method for assaying pine tree nematode for infection by a pine nematode disease assay kit comprising a composition for attracting pine tree nematodes.

In addition, the Haitian pheromone trap and insect monitoring system using the same is known in the Republic of Korea Patent Publication No. 10-2009-0043788, but it attracts insects to the trap by using the species pheromone and then combines the IT technology using the sensor and communication It relates to a method of monitoring the insect.

In addition, the method of controlling the sky cow to mediate pine ash nematode using a sticky trap is known in the Republic of Korea Patent Publication No. 10-2014-0141538, which relates to a method of controlling skyworm adults to mediate pine ash nematode using a sticky trap will be.

On the other hand, the inventors of the present invention has been filed as a new method of capturing the pine worms and pine control insects using the insect pheromone has been filed in the Republic of Korea Patent Application No. 10-2015-0021843, which is a European sol -(Undecyloxy) ethanol relates to a method for preparing a pine salt capture agent and a method for controlling pine tree nematode disease using the capture agent.

The present invention is a composition for controlling pine tree nematode disease, which attracts and captures a saltwater cow and / or a northern saltwater cow, and comprises 2- (dodecyloxy) ethanol, an aggregated pheromone isolated and identified from the saltwater cow, as an active ingredient. An object of the present invention is to provide a composition for controlling pineal nematode disease and a method of manufacturing the same.

In addition, the present invention is attracting and capturing the pineal nematode disease control pine tree and / or northern sea salt sky cow, the composition for pine reptile nematode disease control composition containing 2- (dodecyloxy) ethanol as an active ingredient is adsorbed on a super absorbent resin. It is an object of the present invention to provide a biological control method of pine ash nematode disease using the same.

The present invention is a composition for the control of pine reptile disease that attracts and captures the saltwater cow and / or the northern saltwater cow, and 2- (dodecyloxy which is an aggregated pheromone isolated and identified from the saltwater cow (particularly, Korean saltwater cow). ) Provides a composition for controlling pine ash nematode disease comprising ethanol as an active ingredient. 2- (dodecyloxy) ethanol has the structure of:

Formula

In the composition for controlling pineal nematode disease of the present invention, the content of 2- (dodecyloxy) ethanol, which is aggregated pheromone, is preferably 0.6 to 4.5% (v / v) based on the total composition.

Pine ash nematode disease control composition of the present invention may further comprise ethanol, alpha-pinene (α-pinene), or ethanol and alpha pinene in addition to 2- (dodecyloxy) ethanol aggregate pheromone. Based on the total composition, the content of ethanol is preferably 3-6% (v / v), and the content of alpha-pinene is preferably 10-20% (v / v). 2- (dodecyloxy) ethanol is sufficient for attracting and capturing the saltwater oxen, but 2- (dodecyloxy) ethanol and ethanol and / or alpha-pinene together when attracting and capturing the northern saltwater cow. Synergistic effect is noticeable when used. For example, when 2- (dodecyloxy) ethanol and alpha-pinene are used together, the synergistic effect is about twice as high as when only 2- (dodecyloxy) ethanol is used.

The present invention also provides a pineal nematode disease control and / or northern sea salted sky cow attractant and capture agent, wherein the pine nematode disease control composition is adsorbed on a super absorbent resin. The superabsorbent polymer may be hydrophilic or hydrophobic, and an embodiment of the present invention uses a hydrophilic polymer based on hydrophilic polyacrylic acid.

In addition, the present invention is a method for producing a pine reptile disease control composition for attracting and capturing the pine salt beetle and / or the northern salt beetle, (a) 2- (dodecyloxy) ethanol which is aggregate pheromone from Korean sea beetle Separating and identifying; And (b) mixing the 2- (dodecyloxy) ethanol with purified water or a volatile organic solvent and purified water. In the method for preparing a composition for controlling pineal nematode disease according to the present invention, a volatile organic solvent may not be used, and in the case of using a volatile organic solvent, C3 to C6 alcohol (for example, propanol, isopropanol, n-butanol, etc.) Preference is given to using. As for the volatile organic solvent, it is more preferable that it is 15 to 25% (v / v). Meanwhile, in step (b), ethanol, alpha-pinene, or ethanol and alpha-pinene may be further added.

In addition, the present invention is a biological control method of pine worm nematode disease for attracting and capturing the pine salt beetle and / or northern salt beetle, the pineal beetle for controlling pine needles nematode disease according to the present invention and / or northern salted beetle attractant / capture agent It provides a method for biological control of pine ash nematode disease, characterized in that it is left in the pine forest.

According to the present invention, 2- (dodecyloxy) ethanol, an isolated pheromone isolated and identified from Korean quinine ravine cows, alone or in combination with ethanol / alpha-pinene, attracts and captures pineal lobsters and northern clam vines. It is remarkable in that it has an excellent effect in controlling the disease, and can be used to control pineal nematode disease spreading media by environmentally friendly biological methods.

FIG. 1 illustrates an apparatus for adsorbing a volatile substance secreted by a Korean saltwater bovine into a Parapak-Q filter.

FIG. 2 is a graph showing the results of gas chromatography-mass spectrometry (GC-MASS) identifying volatiles adsorbed through the apparatus of FIG. 1.

Figure 3 illustrates the use of the pineal nematode disease control agent and / or northern salted sky cow attractant and capture agent in which the pineal nematode disease control composition of the present invention is adsorbed on a super absorbent resin.

Figure 4 is a graph showing the attracting and capturing results of the sea bream using the composition for controlling pine ash nematode disease according to the present invention.

5 is a graph showing the attracting and capturing results of the northern bearded sky cow using the composition for controlling pine ash nematode disease according to the present invention.

The following examples are intended to illustrate the invention and should not be construed as limiting the scope of the invention to the following examples.

Example

To prepare a pine ash nematode disease control composition showing the composition of Table 1.

Table 1

Example No.	2- (dodecyloxy) ethanol	ethanol	Alpha-pinene	Volatile Organic Solvents	Purified water
One	4.5	0	0	20.5 (isopropanol)	75
2	0.6	5	14.4	0	80
3	1.2	4.9	13.9	0	80
4	2.3	4.7	13	0	80

* Unit:% (v / v) [based on total composition]

2- (dodecyloxy) ethanol is an essential active ingredient that has been isolated and identified from Korean saltwater bovine cattle, and optional active ingredients ethanol and alpha-pinene are those obtained from ordinary pine.

In Example 2-4, a volatile organic solvent was not used. In Example 1, isopropanol was used as the volatile organic solvent to exclude the effect of ethanol, which is an active ingredient.

Aggregation of pheromone 2- (from Korean domestic bearded cattle Dodecyloxy Identification of Ethanol

(1) Identification method

One side of the 10 mL syringe was clogged with glass wool, and then filled with 5 mL of activated charcoal, and again with glass wool to prepare a charcoal filter. One side of the Pasteur pipet was closed with glass wool, and then 200 mg of Porapak Q (Waters, mesh size 50-80) was charged. Porapak Q filter was prepared by blocking the opposite side again with glass wool (Fig. 1). After finishing the dessert, 5 male and 5 female Korean sea salted oxen were placed in a 2 L container with fresh pine branches and volatiles released for 24 hours were adsorbed on a Porapak Q filter. Experimental conditions were 16 hours of light, 8 hours of cancer, and 25 ℃ of temperature. The control group was put in a 2 L container and tested under the same conditions. In order to elute the adsorbed volatiles in the Porapak Q filter, the Porapak Q filter was washed with 5 mL of dichloromethane and again with 5 mL of pentane.

(2) Identification result

The adsorption volatiles secreted from Korean saltwater cows were analyzed by gas chromatography-mass spectrometry (GC-Mass) and compared with the control group (pine shoots) (Pine shoots + sea salt male) (Males) was detected a new aggregate pheromone (*) (Fig. 2).

Aggregate Pheromone 2- ( Dodecyloxy Preparation of Sea Salt and / or Northern Salt Sky Cattle and Ethanol Containing Ethanol

Hydrophilic polyacrylic acid (polyacrylate) after mixing the components of Table 1 (purified water is to control the volatilization rate), including 2- (dodecyloxy) ethanol, the aggregated pheromone isolated and identified from the Korean sol Each of 50 superabsorbent polymers in the form of a crystal ball (base crystal ball) was bound and stored in a 50 mL tube was used as a capture agent (Fig. 3).

Experimental Example  One: Pine tree nematode disease  Attracting and capturing the saltwater beetle and / or the northern beetle for control To  Attracting and capturing the used sea salt

In the pine forests of Chogok-dong, Heunghae-eup, Buk-gu, Pohang-si, Gyeongsangbuk-do, Korea, for two weeks from July 4 to July 18, 2014, using a sea salt and / or northern salted sky cow for attracting pine reptile diseases according to the present invention. Attracting and capturing the saltwater sky cow was carried out. As shown in FIG. 4, H consists of 14.8% (v / v) alpha-pinene, 5.2% (v / v) ethanol, 80.0% (v / v) water, and P is 2- (dodecyloxy Ethanol 4.5% (v / v), isopropanol 20.5% (v / v), water 75.0% (v / v), H + P is 2- (dodecyloxy) ethanol 2.3% (v / v) , Alpha-pinene 13.0% (v / v), ethanol 4.7% (v / v), water 80.0% (v / v). Using H, P and H + P, respectively, in pineal nematode disease control and northern saltwater cow attractants and capture agents that bound to polyacrylic acid-based superabsorbent resins, H seaweed was not captured. Forty P, H + P capture agents were used to capture a total of 40 perennial sea cows, including 27 females and 13 males.

Experimental Example  2: Pine tree nematode disease  Attracting and capturing the saltwater beetle and / or the northern beetle for control To  Attracting and capturing the Northern Bear Sky Cattle

Attracting and capturing the northern bearded sky cow in Yasan, Hyeondong-myeon, Cheongsong-gun, Gyeongsangbuk-do, Korea, for two weeks from May 8 to May 22, 2015, using a sea salt and northern salt sea cow for controlling pine reptile diseases according to the present invention. Was carried out. As shown in FIG. 5, C was not attracted to the Northern Bearish Sky cow as a control, and H was composed of ethanol and alpha-pinene, and 13 females including 9 females and 4 males were attracted and captured. Is composed only of 2- (dodecyloxy) ethanol, which attracted and captured a total of 19 animals including 14 females and 5 males, and H + P is composed of ethanol, alpha-pinene and 2- (dodecyloxy) ethanol. A total of 34 animals, including 23 females and 11 males, were attracted and captured.

As can be seen through Experimental Examples 1 and 2, the 2- (dodecyloxy) ethanol alone, which was isolated and identified from Korean saltwater bovine cows, was excellent in attracting and capturing the saltwater bovine cow and / or the northern saltwater bovine cow. And synergistic effects are apparent when ethanol and / or alpha-pinene are used together.

Claims (10)

1. A pineal nematode disease control composition that attracts and captures a saltwater cow, a northern saltwater cow, or a saltwater cow and a northern saltwater cow, and effectively uses 2- (dodecyloxy) ethanol, an aggregated pheromone isolated and identified from the saltwater cow. Pine ash nematode disease control composition comprising as a component.
2. According to claim 1, Ethanol, alpha-pinene, or pineapple nematode disease control composition further comprises ethanol and alpha pinene.
3. The composition of claim 1, wherein the content of 2- (dodecyloxy) ethanol is 0.6 to 4.5% (v / v) based on the total composition.
4. The method according to claim 2, wherein the content of ethanol is 3-6% (v / v) and alpha-pinene is 10-20% (v / v), based on the total composition. Control composition.
5. The pineal nematode disease control saltwater cow, northern saltwater sky cow, or pineal salt sky cow and northern saltwater sky cow, characterized in that the pineal nematode disease control composition according to claim 1 is adsorbed on a hydrophilic or hydrophobic superabsorbent resin. Capture agent.
6. The method of claim 5,

   The superabsorbent polymer is a polyacrylic acid-based hydrophilic superabsorbent resin, pineal nematode disease control salted cow, northern salted fish, or salted salted fish and northern salted fish.
7. As a method for producing a composition for controlling pineal nematode disease, which attracts and captures a pine salted cow, a northern salted cow, or a pine salted cow and a northern salted cow,

   (a) separating and identifying 2- (dodecyloxy) ethanol, which is an aggregated pheromone, from Korean quinine brine; And

   (b) mixing the 2- (dodecyloxy) ethanol with purified water, or a volatile organic solvent and purified water.
8.  The method of claim 7, wherein

   Ethanol, alpha-pinene, or ethanol and alpha-pinene in the step (b) further comprises the method for producing a composition for pine ash nematode disease control.
9. The method of claim 7, wherein

   In the step (b), the volatile organic solvent is C3 to C6 alcohol production method of pine pine nematode disease control composition, characterized in that.
10. As a biological control method of pine barley nematode disease, which attracts and captures the saltwater cow, the saltwater cow, or the saltwater cow and the saltwater cow,

    A method for biological control of pine bar nematodes disease, characterized in that the pineal nematode disease, pine tree beetle, or pine tree beetle and northern beetle beetle are attracted to the pine forest. .

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