WO2016158487A1

WO2016158487A1 - Sensing sensor and sensing device

Info

Publication number: WO2016158487A1
Application number: PCT/JP2016/058667
Authority: WO
Inventors: 茎田啓行
Original assignee: 日本電波工業株式会社
Priority date: 2015-03-30
Filing date: 2016-03-18
Publication date: 2016-10-06
Also published as: JP2016188829A

Abstract

The objective of the present invention is to provide a sensing sensor 2 and a sensing device with which it is possible to restrict a deterioration in measurement sensitivity, and to detect or quantify a sensing target. A supply solution flows from a filler opening 23 through a flow path 57 on one surface side of a crystal oscillator 4, to an effluent flow path 53, and a sensing target contained in the supply solution is adsorbed onto an adsorption film 48 provided on the crystal oscillator 4. Further, after rising through the effluent flow path 53 as a result of the hydraulic pressure of the supply solution introduced into the filler opening 23, the supply solution is absorbed by means of an outlet side capillary member 56. When the amount of supply solution on the filler opening side decreases, the hydraulic pressure decreases and it becomes difficult for the supply solution to rise through the effluent flow path 53, thereby forming a state in which the supply solution that is absorbed and pulled into the outlet side capillary member 56 is separated from the supply solution on the flow path 57 side. Thus, even if a buffer solution is supplied to the sensing sensor before the sample solution to be sensed is supplied as the supply solution, dilution of the sample solution as a result of inflow of the buffer solution can be restricted, and a deterioration in the measurement sensitivity can be suppressed.

Description

Sensing sensor and sensing device

The present invention relates to a sensing sensor and a sensing device for sensing a sensing object contained in a sample liquid based on an oscillation frequency of a piezoelectric vibrator.

In the clinical field, for example, a simple method called POCT (Point-of-care-testing) represented by self-monitoring of blood glucose level is widespread. As an example of this method, Patent Document 1 describes a sensing sensor using a QCM (Quartz Crystal Microbalance). A quartz resonator is placed on a wiring board, and an upper case and a lower case are engaged with each other. Thus, a structure is disclosed in which an accommodation space for the sample liquid is formed between the upper case and the crystal resonator. As a technique for increasing the sensitivity of QCM, there is a twin sensor that performs differential measurement. In this technique, two electrodes are formed symmetrically with respect to the flow path on a single crystal, and the sample solution is configured to flow simultaneously and similarly to the electrodes to cancel ambient external noise. *

As shown in FIG. 14 for an example of this sensing sensor, a crystal resonator 91 is provided so as to close a through hole 90 formed in the wiring substrate 9, and the sample liquid flow is provided on one surface side of the crystal resonator 91. A path 92 is formed. The sample liquid supplied from the liquid receiving portion 931 formed on the cover 93 is configured to circulate from the inlet side capillary member 94 to the waste liquid region 96 through the flow path 92 and the outlet side capillary member 95. Has been. Reference numeral 97 denotes a film provided on the other surface side of the substrate 9 so as to close the through hole 90. *

When measuring an object to be sensed, first, a buffer solution is supplied from the liquid receiving portion 931, and the flow path 92 is filled with the buffer solution to stabilize the frequency. Next, the sample liquid is supplied from the liquid receiving portion 931, and the supply of the sample liquid moves the buffer solution in the flow path 92 from the outlet side capillary member 95 to the waste liquid region 96, and the sample liquid reaches the flow path 92. . In this way, the sample liquid flows in a laminar flow in the flow path 92, and the buffer solution moves so as to be pushed out to the waste liquid storage unit 96 by the sample liquid. Then, the frequency is detected when the flow path 92 is filled with the sample solution, and the sensing object in the sample solution is detected and quantified based on the change in the frequency at this time. *

For example, when all the sample liquid in the liquid receiving part 931 moves to the inlet side capillary member 94 and the sample liquid disappears from the liquid receiving part 931, the sample liquid fills the inside of the flow path 92 and passes through the outlet side capillary member 95. An amount of sample liquid reaching the waste liquid region 96 is supplied. Thus, in a state where all the sample liquid in the liquid receiving portion 931 has moved to the inlet side capillary member 94, the sample liquid is stationary due to the holding force of the capillary phenomenon. However, the buffer solution is stored in the waste liquid region 96, and the waste liquid region 96 and the outlet side capillary member 95 are in contact with each other. Therefore, since the channel 92 in which the sample solution exists and the waste liquid region 96 in which the buffer solution exists are connected, the buffer solution flows backward toward the outlet side capillary member 95 and gradually moves toward the channel 92 side. There is a risk. When this phenomenon occurs, there is a concern that the buffer solution is gradually mixed with the sample solution in the flow path 92, and the sample solution is diluted with the buffer solution to reduce the measurement sensitivity.

JP 2012-145 566 A

The present invention has been made under such circumstances, and an object thereof is to provide a sensing sensor capable of detecting or quantifying a sensing object while suppressing a decrease in measurement sensitivity.

The sensing sensor of the present invention includes a wiring board having a recess formed on one surface side,
The piezoelectric piece is provided with an excitation electrode that is electrically connected to the conductive path of the wiring board, and an adsorption film that adsorbs a sensing object in the sample liquid is formed on one side, and the vibration region is A piezoelectric vibrator fixed to the wiring board in a state where the recess is closed so as to face the recess,
A flow path that is provided so as to cover a region on one surface side of the wiring substrate including the piezoelectric vibrator, and allows the sample liquid to flow from one end side to the other end side on the one surface side of the piezoelectric vibrator with the piezoelectric vibrator. A flow path forming member for forming
An inlet for injecting a sample solution from above into one end of the flow path;
A waste liquid channel connected to the other end of the flow channel so as to extend upward, and a sample liquid rising by a liquid pressure of the sample liquid injected into the injection port;
A capillary member that is provided on the downstream side of the waste liquid flow path and absorbs the sample liquid that has risen through the waste liquid flow path;
The sample liquid rising in the waste liquid flow path is separated into an upstream side and a downstream side by absorption of the sample liquid by the capillary member and gravity.

The detection sensor of the present invention may include an absorption member provided on the downstream side of the capillary member for absorbing the sample liquid flowing through the capillary member, and the inlet is a porous member It is good also as comprising by the hole of this. *

The sensing device of the present invention includes the above-described sensing sensor and a measuring device.

The sensing sensor of the present invention has an adsorption film in which a sample liquid flows from an inlet to a waste liquid flow path via a flow path on one side of the piezoelectric vibrator, and a sensing object contained in the sample liquid is provided on the piezoelectric vibrator. To be adsorbed. Further, the sample liquid is absorbed by the capillary member after rising the waste liquid flow path by the liquid pressure of the supply liquid injected from the injection port. When the amount of supply liquid on the inlet side decreases, the liquid pressure that raises the supply liquid to the waste liquid flow path decreases, and the sample liquid absorbed by the capillary member and pulled by the capillary member is separated from the sample liquid on the flow path side. A formed state is formed. Therefore, even when a buffer solution is supplied to the sensing sensor prior to the sample solution, dilution of the sample solution with the buffer solution can be suppressed, and a decrease in measurement sensitivity can be suppressed.

1 is a perspective view of a sensing device using a sensing sensor according to the present invention. It is a disassembled perspective view of a sensing sensor. It is the disassembled perspective view which showed the upper surface side of each part of a detection sensor. It is the disassembled perspective view which showed the lower surface side of a part of detection sensor. It is a vertical side view of the sensing sensor. It is a top view of a sensing sensor. It is a block diagram which shows the structure of a detection sensor. It is explanatory drawing which shows the flow of the supply liquid supplied to the said sensor. It is explanatory drawing which shows the flow of the supply liquid supplied to the said sensor. It is explanatory drawing which shows the flow of the supply liquid supplied to the said sensor. It is explanatory drawing which shows the flow of the supply liquid supplied to the said sensor. It is explanatory drawing which shows the flow of the supply liquid supplied to the said sensor. It is explanatory drawing which shows the flow of the supply liquid supplied to the said sensor. It is a longitudinal cross-sectional view which shows the prior art example of a sensing sensor.

Hereinafter, a sensing device using a sensing sensor according to an embodiment of the present invention will be described. This sensing device uses a microfluidic chip, and can detect the presence or absence of an antigen such as a virus in a sample liquid obtained from, for example, a wiping liquid of a human nasal cavity, and determine the presence or absence of a human virus infection. It is configured as follows. As shown in the external perspective view of FIG. 1, the sensing device includes a main body 12 and a sensing sensor 2. The detection sensor 2 is detachably connected to an insertion port 17 formed in the main body 12. On the upper surface of the main body 12, a display unit 16 constituted by, for example, a liquid crystal display screen is provided. The display unit 16 is, for example, an output frequency of an oscillation circuit provided in the main body 12 or a change in frequency described later. Displays the measurement results such as minutes or the presence or absence of virus detection. *

Next, the detection sensor 2 will be described. 2 shows a perspective view of the detection sensor 2 shown in FIG. 1 with the upper cover 21 removed, and FIGS. 3 and 4 show the front side (upper surface side) and some members of each member of the detection sensor 2, respectively. FIG. 5 and FIG. 6 show a sectional view of the sensor 2 and a plan view with the upper cover body 21 removed, respectively.
The detection sensor 2 includes a container 20 including an upper cover body 21 and a lower case 22. A wiring board 3 having a shape extending in the length direction is provided above the lower case 22, and is inserted into the insertion port 17 of the main body 12 on one end side in the length direction of the wiring board 3. The insertion part 31 is formed. In the following description, the insertion part 31 side of the sensor 2 is defined as the rear side, and the other end side is defined as the front side.

As shown in FIG. 3, a through hole 32 is formed at a position on the front side of the wiring board 3. The wiring board 3 is disposed so that the through hole 32 is closed by the bottom surface of the lower case 22 and the insertion portion 31 protrudes outside the lower case 22. Three wirings 34 to 36 extending in the length direction are provided on the surface side of the wiring board 3, and one end side of each wiring 34 to 36 is connected to the

terminal portions

342, 352, 362 is formed. Further,

terminal portions

341, 351, and 361 are formed at the outer edges of the through holes 32 on the other ends of the wirings 34 to 36, respectively. Further, two holes 33 for determining the horizontal position of the wiring board 3 are formed in the width direction in front of the through hole 32 in the wiring board 3. *

Next, the piezoelectric vibrator, for example, the crystal vibrator 4 will be described. As shown in FIGS. 3 and 4, the crystal resonator 4 includes, for example, an AT-cut disc-shaped crystal piece 41, and the surface of the crystal piece 41 is formed of, for example, Au (gold).

Excitation electrodes

42A and 42B are provided, and

excitation electrodes

43A and 43B are provided in strips on the back side of the crystal piece 41 so as to correspond to the

excitation electrodes

42A and 42B, respectively. The

excitation electrodes

42A and 42B on the front side are connected to each other at one end in the length direction, and then connected to one end of the wiring 44. The other end of the wiring 44 is routed around the lower surface side periphery of the crystal unit 4. After that, an electrode 45 is formed. On the surface of one excitation electrode 42A, for example, an adsorption film 48 made of an antibody for adsorbing a sensing object that is an antigen is formed. Further, the surface of the other excitation electrode 42B is exposed without the adsorption film 48 formed. Further, one end of

wirings

46 and 47 extending to the periphery of the crystal resonator 4 is connected to the

excitation electrodes

43A and 43B provided on the lower surface side of the crystal resonator 4, and the other end side of each

wiring

46 and 47 is a crystal. Electrodes 46 </ b> A and 47 </ b> A are formed on the lower surface side periphery of the vibrator 4.
The crystal unit 4 is arranged such that the

excitation electrodes

43A and 43B on the lower surface side face the through holes 32 of the wiring board 3, and the electrodes 46A and 47A are connected to the

terminal portions

341 and 361 by a conductive adhesive, respectively. 45 is connected to the terminal portion 351 by a conductive adhesive.

A flow path forming member 5 is provided on the upper surface side of the wiring board 3. The flow path forming member 5 is constituted by a plate-like member having a thickness of, for example, 2.0 mm made of PDMS (polydimethylsiloxane), for example. At a position near the front of the flow path forming member 5, a hole 58 for aligning the flow path forming member 5 is located at a position corresponding to the hole 33 formed in the wiring board 3. 5 is provided so as to penetrate through in the thickness direction. *

As shown in FIG. 4, a substantially circular recess 54 is formed on the lower surface side of the flow path forming member 5 so as to accommodate the crystal resonator 4. On the lower surface side of the flow path forming member 5,

grooves

253, 263, and 273 are formed in which the wirings 34 to 36 formed on the wiring board 3 are accommodated and communicated with the recesses 54, respectively. The concave portion 54 is provided with an enclosing portion 51 that partitions and forms a flow path 57 of the sample solution between the surface of the crystal resonator 4 when the flow path forming member 5 is pressed toward the wiring substrate 3 side. The surrounding portion 51 is configured by an annular protruding portion whose outer edge is formed in an oval shape so that the length direction thereof faces in the front-rear direction of the detection sensor 2. The surrounding portion 51 is provided so as to protrude to a thickness of 300 μm from the concave portion 54, and a region inside the surrounding portion 51 is a flat surface having the same height as the concave portion 54. In addition, the width of the inner region of the enclosing portion 51 is configured so as to spread radially from the rear side, then become a constant width in the middle flow area, and then gradually narrow toward the front side. *

The flow path forming member 5 has a through hole 52 having a diameter of 1.5 mm that opens at the rear end of the inner region of the surrounding portion 51 and penetrates in the thickness direction. The flow path forming member 5 is provided with a waste liquid flow path 53 having a diameter of 1.5 mm that opens at the front end of the inner region of the surrounding portion 51 and penetrates in the thickness direction. When the flow path forming member 5 is arranged so that the hole portion 58 is aligned with the hole portion 33 provided in the wiring substrate 3, the surrounding portion 51 is arranged on the upper surface of the crystal resonator 4, and the excitation electrode of the crystal resonator 4 is 42A. 42B are aligned in the center of the inner region of the surrounding portion 51, and the lower surface side of the inner region of the surrounding portion 51 is closed by the crystal unit 4. A region sandwiched between the flow path forming member 5 and the crystal unit 4 and surrounded by the surrounding portion 51 corresponds to the flow path 57. *

The upper cover body 21 is provided so as to cover the wiring board 3 and the flow path forming member 5 excluding the insertion portion 31 from above. An inlet 23 inclined in a mortar shape is formed on the upper surface side of the upper cover body 21. As shown in FIG. 4, a pressing portion 80 for pressing the flow path forming member 5 against the wiring board 3 is provided on the back surface side of the upper cover body 21. The pressing portion 80 is configured, for example, in a substantially box shape, and when the upper cover body 21 is fitted into the lower case 22 and locked together, the lower surface presses the entire upper surface of the flow path forming member 5 vertically downward. To do. The pressing portion 80 is provided with a through hole 81 that penetrates the injection port 23 at a position corresponding to the through hole 52. In addition, a notch 82 is formed from the position corresponding to the waste liquid flow path 53 toward the front side to secure an installation area for an outlet side capillary member 56 described later. Further, the pressing portion 80 is provided with a fixing column 83 for positioning the flow path forming member 5 and the wiring board 3 inserted into

holes

58 and 33 provided in the flow path forming member 5 and the wiring board 3 respectively. It has been. *

As shown in FIGS. 3 and 5, the through hole 52 is closed, and the upper end thereof is exposed to the injection port 23 through the through hole 81, and the lower end is made of a porous member so as to enter the flow path 57. An inlet side capillary member 55 is detachably provided. The inlet-side capillary member 55 is, for example, a cylindrical member, and is formed of a chemical fiber bundle such as polyvinyl alcohol (PVA), and is a porous member in which a large number of holes having a diameter of about 80 μm are formed. In the inlet-side capillary member 55, the supply liquid flows through the gap between the fibers of the chemical fiber bundle by capillary action. In this embodiment, the injection port 23, the through

holes

52 and 81, and the inlet side capillary member 55 correspond to an injection path.

In addition, the waste liquid channel 53 is provided with an outlet side capillary member 56. The outlet side capillary member 56 is configured to be porous in which a large number of holes having a diameter of, for example, about 80 μm are formed by a bundle of chemical fibers such as polyvinyl alcohol (PVA), and a horizontal portion 561 extending in a wall shape in the front-rear direction, and the horizontal portion A vertical portion 562 extending 0.5 mm downward from 561 is formed in a substantially L shape. As shown in FIG. 6, when the vertical part 562 is inserted into the waste liquid channel 53, a gap is formed between the left and right surfaces of the vertical part 562 and the inner wall of the waste liquid channel 53. The width of the outlet-side capillary member 56 is set to a width that does not block the opening of the waste liquid channel 53 when the outlet-side capillary member 56 absorbs water and expands, and is set to, for example, a width of 0.5 mm. *

A rectangular absorbent member 7 made of, for example, PVA is provided on the downstream side of the outlet side capillary member 56, and the downstream end portion of the outlet side capillary member 56 is inserted into a cut 72 formed in the absorbent member 7. Has been. A case body 73 for preventing liquid leakage from the absorbing member 7 is provided outside the absorbing member 7. Further, a support member 75 for supporting the outlet side capillary member 56 is provided between the flow path forming member in the lower case 22 and the case body 73, and the outlet side capillary member 56 is a support member 75. It is inserted into and supported by the slit 74 formed in the above. *

The sensing sensor 2 configured as described above has an excitation electrode provided on the crystal resonator 4 as shown in FIG. 7 when the insertion portion 31 of the wiring board 3 is inserted into the insertion port 17 of the main body portion 12. 42A and 43A become the 1st vibration area | region 61, are connected to the 1st oscillation circuit 63, and oscillate. A region sandwiched between the excitation electrodes 42 </ b> B and 43 </ b> B of the crystal unit 4 becomes a second vibration region 62, which is connected to the second oscillation circuit 64 and oscillates. In the sensing device of the present invention, channel 1 connecting data processing unit 66 and first oscillation circuit 63 and channel 2 connecting data processing unit 66 and second oscillation circuit 64 are provided by switch unit 65. Intermittent oscillation that is alternately switched is performed, and the oscillation frequencies F1 and F2 of the channels 1 and 2 are measured. Interference between the two

vibration regions

61 and 62 of the sensor 2 is avoided, and a stable frequency signal can be acquired. These frequency signals are time-divided, for example, and taken into the data processing unit 66. The data processing unit 66 calculates the frequency signal as, for example, a digital value, performs arithmetic processing based on the time-division data of the calculated digital value, and displays, for example, a calculation result such as the presence or absence of an antigen on the display unit 16. . *

A method for determining the presence or absence of an object to be detected in the sample liquid using this sensing device will be described with reference to FIGS. In the specification, the liquid supplied from the inlet 23 of the detection sensor 2 is collectively referred to as “supply liquid”, and the supply liquid including the detection target is “sample liquid”, the detection target supplied before the sample liquid. A feed solution that does not contain a substance is referred to as a “buffer solution”. In FIGS. 8 to 13, the buffer solution is indicated by dots having a low density, and the sample solution is indicated by dots having a high density. First, the detection sensor 2 is connected to the main body 12, and the user drops, for example, a buffer solution that is made of, for example, physiological saline and does not include a detection target, into the injection port 23 using a syringe. The buffer solution is absorbed by the inlet side capillary member 55, flows through the inlet side capillary member 55 by capillary action, flows into the flow path 57, and is supplied to the rear surface of the crystal unit 4. *

Since the surface of the crystal piece 41 constituting the crystal unit 4 is hydrophilic, the buffer solution supplied to the channel 57 wets and spreads in the channel 57. The buffer solution absorbed in the inlet side capillary member 55 following the buffer solution spread in the channel 57 is drawn to the surface of the crystal piece 41 by surface tension as shown in FIG. The buffer solution flows to 57 continuously. Since the

excitation electrodes

42A and 42B are arranged side by side in the vicinity of the midstream portion of the flow channel 57, the buffer solution simultaneously flows on the surfaces of the

excitation electrodes

42A and 42B at a constant speed. When the buffer solution flowing through the flow path 57 is supplied to the surfaces of the

excitation electrodes

42A and 42B, the

excitation electrodes

42A and 42B are formed symmetrically when viewed from the inlet side to the outlet side of the flow path 57. Equally affected by water pressure. As a result, both the oscillation frequencies of the first vibration region 61 and the second vibration region 62 are equally reduced. *

Then, after the buffer solution fills the flow channel 57, as shown in FIG. 9, the buffer solution rises in the waste liquid flow channel 53 by the siphon principle based on the liquid pressure of the buffer solution supplied to the inlet 23. When the buffer solution rises in the waste liquid flow channel 53 and the buffer solution and the outlet side capillary member 56 come into contact with each other, the buffer solution is absorbed into the outlet side capillary member 56 at a stretch, and is thus accelerated. As a result, the difference between the flow rate of the buffer solution absorbed by the outlet side capillary member 56 and the flow rate of the buffer solution that rises in the waste fluid channel 53 causes the buffer solution on the channel 57 side and the outlet side as shown in FIG. The buffer solution absorbed by the capillary member 56 is separated.

At this time, if the buffer solution remains in the injection port 23, the buffer solution on the flow channel 57 side is pushed away by the force of the buffer solution flowing from the injection port 23 into the inlet capillary member 55, and the waste liquid flow channel 53 is raised. And rises until it contacts the outlet side capillary member 56. When the buffer solution comes into contact with the outlet side capillary member 56, the buffer solution is again absorbed by the outlet side capillary member 56 again, and the buffer solution on the channel 57 side and the buffer solution absorbed by the outlet side capillary member 56 are separated. . The buffer solution is absorbed by the outlet side capillary member 56, and the buffer solution on the channel 57 side is separated from the buffer solution absorbed by the outlet side capillary member 56, and the buffer solution in the channel 57 is used as the waste fluid channel. The operation of raising 53 is repeated until all the buffer solution in the inlet 23 flows into the inlet side capillary member 55. When all of the buffer solution in the inlet 23 flows into the inlet side capillary member 55, the inlet side capillary member 55 tries to hold the buffer solution, so that the buffer solution flows out from the inlet side capillary member 55 to the flow path 57. Stops and the flow of buffer stops. Therefore, as shown in FIG. 11, the buffer solution in the flow channel 57 stops in a state where the buffer solution on the flow channel 57 side and the buffer solution absorbed by the outlet side capillary member 56 are separated. *

Further, the buffer solution absorbed by the outlet side capillary member 56 will be described. The buffer solution is gradually absorbed every time it goes up the waste liquid channel 53 and contacts the outlet side capillary member 56, and gradually fills from the upstream side to the downstream side of the outlet side capillary member 56. Then, when the absorbed supply liquid reaches the downstream end of the outlet side capillary member 56, it is absorbed by the absorbing member 7 connected downstream of the outlet side capillary member 56. When the supply liquid is absorbed by the absorbing member 7 from the outlet side capillary member 56, a force flowing from the upstream side to the downstream side in the outlet side capillary member 56 acts on the supply liquid inside the outlet side capillary member 56. As a result, the supply liquid absorbed by the outlet side capillary member 56 continuously flows into the absorption member 7. *

Subsequently, for example, the same amount of sample solution as the buffer solution is supplied to the inlet 23. When the injected sample liquid flows into the inlet side capillary member 55, the buffer solution flows toward the downstream side of the flow path 57 as shown in FIG. Further, since the sample solution tends to flow into the inlet side capillary member 55 due to the weight, the buffer solution rises in the waste liquid channel 53 and contacts the outlet side capillary member 56 to be absorbed. Thereby, the buffer solution is absorbed by the outlet side capillary member 56, and the buffer solution of the outlet side capillary member 56 is separated from the buffer solution and the sample solution on the channel 57 side. *

Thereafter, the remaining buffer rises in the waste liquid channel 53 and comes into contact with the outlet side capillary member 56, and the buffer solution is absorbed by the outlet side capillary member 56, and the buffer solution of the outlet side capillary member 56 and the channel 57 side The operation of separating the supply liquid is repeated, and the buffer solution remaining in the flow path 57 is absorbed by the outlet side capillary member 56. In the channel 57, the buffer solution is gradually pushed downstream, and the liquid phase filling the channel 57 is replaced with the sample solution from the buffer solution. *

Further, after the liquid phase in the flow path 57 is replaced with the sample liquid, the sample liquid remaining in the inlet 23 tends to flow into the inlet side capillary member 55, so that the sample liquid in the flow path 57 becomes the waste liquid flow path 53. And the sample liquid rising through the waste liquid channel 53 is absorbed by the outlet side capillary member 56 and absorbed by the sample liquid on the channel 57 side and the outlet side capillary member 56. The operation of separating the sample liquid is repeated until all the sample liquid supplied to the inlet 23 flows into the inlet side capillary member 55. Thereafter, when there is no sample liquid in the inlet 23, the flow of the sample liquid from the inlet side capillary member 55 to the flow path 57 stops, and the sample liquid hardly rises on the waste liquid flow path 53 side, as shown in FIG. Then, the sample liquid absorbed in the outlet side capillary member 56 and the sample liquid on the flow path 57 side are stopped in a separated state. *

When the liquid phase in the flow path 57 is replaced with the sample liquid, if the sample object contains a sensing object, the sensing object is adsorbed on the adsorption film 48 on the excitation electrode 42A. On the other hand, the sensing object is not adsorbed on the excitation electrode 42B. For this reason, the frequency F1 output from the first oscillation circuit decreases in accordance with the amount of the object to be adsorbed to the adsorption film 48, so that F1-F2 changes. In this way, the presence / absence of a sensing object can be determined based on the change in F1-F2. Further, a relational expression between the change amount of the oscillation frequency difference F1-F2 and the concentration of the sensing object in the sample liquid is acquired in advance, and the change amount between the relational expression and the difference of the oscillation frequency obtained by the measurement From the above, the concentration of the sensing object in the sample solution may be obtained. *

In the above-described embodiment, the supply liquid (buffer solution and sample liquid) injected into the sensing sensor 2 flows through the flow path 57 and then rises in the waste liquid flow path 53 and is absorbed by the outlet side capillary member 56. Thereafter, it flows into the absorbing member 7. At this time, the supply liquid on the flow path 57 side and the supply liquid on the outlet side capillary member 56 side are separated by absorption of the outlet side capillary member 56. Therefore, when the sensing object is measured, the supply liquid on the flow path 57 side and the supply liquid in the outlet side capillary member 56 are not mixed, and the supply liquid on the flow path 57 side does not mix in the outlet side capillary member 56. There is no risk of dilution with the feed solution. Further, when the supply liquid ascends the waste liquid channel 53 and reaches the outlet side capillary member 56, the supply liquid is absorbed at the outlet side capillary member 56 side at a speed higher than the rising speed in the waste liquid channel 53. It flows more easily toward the outlet side capillary member 56 than the passage 53, and hardly flows from the outlet side capillary member 56 to the waste liquid channel 53. For this reason, the supply liquid is unlikely to flow backward from the outlet side capillary member 56 toward the waste liquid flow path 53, and the dilution of the supply liquid is also suppressed from this point. Therefore, it is possible to detect and measure the sensing object in the sample liquid with the measurement sensitivity always high. *

Even when the inlet-side capillary member 55 is not provided, the supply liquid can flow into the flow path 57 from the injection port 23 using gravity, and the waste liquid flow path 53 can be raised, so that the amount of the supply liquid decreases. Since the supply liquid does not rise in the waste liquid flow path 53, the supply liquid absorbed by the outlet side capillary member 56 and the supply liquid on the flow path side can be formed in the same manner, so that the same effect can be formed. Is obtained. *

Further, the absorbing member 7 may not be provided. For example, when the supply liquid is supplied from the injection port 23 and the sample is measured, the supply liquid that has risen in the waste liquid flow path 53 may be absorbed and held by the outlet side capillary member 56.
Furthermore, in the above-described embodiment, the support member 75 that supports the outlet-side capillary member 56 is provided between the absorption member 7 and the flow path forming member 5, but is supported by the notch 72 of the absorption member 7. You may do it. Alternatively, the outlet side capillary member 56 may be supported from the upper cover body 21 side, and for example, the outlet side capillary member 56 may be sandwiched and supported by the notch 82.

2 sensing sensors,
3 Wiring board,
4 Crystal resonator,
5 flow path forming member,
21 upper cover body,
22 Lower case,
23 Inlet,
52, 81 through hole,
53 Waste liquid flow path,
55 inlet side capillary member,
56 outlet side capillary member,
57 channel,
61 first vibration region;
62 Second vibration region

Claims

A wiring board having a recess formed on one side;
The piezoelectric piece is provided with an excitation electrode that is electrically connected to the conductive path of the wiring board, and an adsorption film that adsorbs a sensing object in the sample liquid is formed on one side, and the vibration region is A piezoelectric vibrator fixed to the wiring board in a state where the recess is closed so as to face the recess,
A flow path that is provided so as to cover a region on one surface side of the wiring substrate including the piezoelectric vibrator, and distributes the supply liquid from one end side to the other end side on the one surface side of the piezoelectric vibrator with the piezoelectric vibrator. A flow path forming member for forming
An injection path for injecting the supply liquid from above into one end side of the flow path;
A waste liquid flow path that is connected to the other end side of the flow path so as to extend upward, and the supply liquid rises due to the liquid pressure of the supply liquid injected into the injection path;
A capillary member that is provided on the downstream side of the waste liquid passage and absorbs the supply liquid that has risen through the waste liquid passage; and
A sensing sensor, wherein the supply liquid that has risen in the waste liquid flow path is separated into an upstream side and a downstream side by absorption of the supply liquid by the capillary member and gravity.
An absorption member provided on the downstream side of the capillary member for absorbing the supply liquid flowing through the capillary member.
The sensing sensor according to claim 1 or 2, wherein a porous member is provided in the injection path, and a supply liquid flowing through the porous member is supplied to the flow path.
A sensing device comprising the sensing sensor according to any one of claims 1 to 3 and a measuring instrument.