WO2016149882A1 - Utilisations d'une eau potable traitée à l'aide d'ondes électromagnétiques dans la préparation d'une boisson, d'un produit de soins de santé ou d'un médicament anti-constipation - Google Patents

Utilisations d'une eau potable traitée à l'aide d'ondes électromagnétiques dans la préparation d'une boisson, d'un produit de soins de santé ou d'un médicament anti-constipation Download PDF

Info

Publication number
WO2016149882A1
WO2016149882A1 PCT/CN2015/074758 CN2015074758W WO2016149882A1 WO 2016149882 A1 WO2016149882 A1 WO 2016149882A1 CN 2015074758 W CN2015074758 W CN 2015074758W WO 2016149882 A1 WO2016149882 A1 WO 2016149882A1
Authority
WO
WIPO (PCT)
Prior art keywords
electromagnetic wave
frequency electromagnetic
water
drinking water
kinetic energy
Prior art date
Application number
PCT/CN2015/074758
Other languages
English (en)
Chinese (zh)
Inventor
王卫星
Original Assignee
欢乐海（北京）水业科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 欢乐海（北京）水业科技有限公司 filed Critical 欢乐海（北京）水业科技有限公司
Priority to PCT/CN2015/074758 priority Critical patent/WO2016149882A1/fr
Priority to AU2015387922A priority patent/AU2015387922A1/en
Publication of WO2016149882A1 publication Critical patent/WO2016149882A1/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/48—Treatment of water, waste water, or sewage with magnetic or electric fields

Definitions

  • the invention belongs to the technical field of water treatment, and particularly relates to the use of multi-pole micro-kinetic energy drinking water in preparing beverages, health care products or medicines for laxation.
  • Water is an inorganic substance composed of two elements of hydrogen and oxygen, and is a colorless and odorless transparent liquid under normal temperature and normal pressure. Water is one of the most common substances, an important resource for all life, including human beings, and the most important component of living things. Water plays an important role in the evolution of life. Humans have long begun to understand water, and water is regarded as a basic component in the ancient and simple material view of the East and West.
  • the water molecule itself has its special structural shape, it is composed of two hydrogen and one oxygen ion combined at an angle of 104.5. It is an ionization system with an electronic "magnetic polarity". Water molecules exhibit solid crystals below zero and below zero, which we call ice. However, water is not completely liquid at a temperature of from zero to 60 ° C, but exists in the form of a liquid crystal. However, if the temperature is higher than 60 ° C, the liquid crystal molecules will be destroyed.
  • the form of the diluted solute molecules can be contained in its liquid crystals, which is a generally understood dissolution process.
  • the new discovery is that when a solute molecule is separated from a water molecule by some means (eg, through multiple dilutions and oscillations), the water molecule can still maintain a "shape" memory of the solute molecule. When the body cells are exposed to such water molecules, the "shape" memory of the solute molecule is treated as a true solute molecule, which is the cornerstone of homeopathic therapy with more than 200 years of history.
  • Water is composed of two elements of hydrogen and oxygen, but does not exist in nature as a single water molecule. Instead, a group of water molecules (H 2 O) n is present in nature (ie, the above liquid state). Crystallization), the group of water molecules may be chain, ring, agglomerate or grape-like, with at least five water molecules, and more than a dozen, tens to hundreds.
  • Electromagnetic waves have been used to treat water molecules to increase the energy level of water molecules or to disinfect or prevent and remove dirt.
  • these treatment methods do not control the effect of the treatment well, and the effect of the treated water is mostly limited to drinking, and there is no other additional advantageous effect.
  • the present invention provides a multi-pole micro-kinetic energy drinking water obtained by electromagnetic wave non-contact treatment, a preparation method thereof and use thereof.
  • the present invention provides a multi-pole micro-kinetic energy drinking water prepared by non-contact treatment of drinking water raw water by electromagnetic waves, and the ultraviolet absorption peak is short-waved compared with the raw water of drinking water.
  • the direction shift is 25 nm to 40 nm, preferably 28 nm to 30 nm in the short-wave direction, and most preferably 30 nm or 28 nm in the short-wave direction.
  • the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .
  • the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Cambodia glacier mineral water, Nongfu Spring, Wahaha, and the like.
  • the multi-pole micro-kinetic energy drinking water of the present invention is best consumed or used within 72 hours after preparation.
  • the present invention provides a method for preparing the above-mentioned multi-pole micro-kinetic energy drinking water, which comprises non-contact treatment of drinking water raw water by using electromagnetic waves of low, medium and high frequencies, wherein the frequency of low-frequency electromagnetic waves
  • the range is 30-100 kHz
  • the frequency range of the intermediate frequency electromagnetic wave is 550-720 kHz
  • the frequency range of the high frequency electromagnetic wave is 300-725 MHz.
  • the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Cambodia glacier mineral water, Nongfu Spring, Wahaha, and the like.
  • the low frequency electromagnetic wave has a frequency in the range of 45-75 kHz.
  • the intermediate frequency electromagnetic wave has a frequency range of 600-720 kHz.
  • the high frequency electromagnetic wave has a frequency in the range of 300-425 MHz.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are a sine wave, a square wave, a sharp wave, a sawtooth wave or a trapezoidal wave.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave waveform may be the same or different, preferably the same.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave propagate in the same direction.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed by the electromagnetic wave non-contact treatment of the drinking water raw water treatment by using three different frequencies of low, medium and high frequencies, and then the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave are simultaneously processed.
  • the time for simultaneous treatment using the low frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes; the time for simultaneous treatment using the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes. .
  • the prepared ultraviolet light absorption peak of the multi-pole micro-kinetic energy drinking water is shifted to the short-wave direction by 25 nm to 40 nm, preferably to the short-wave direction by 28 nm to 30 nm, and most preferably to the short-wave direction. 30 nm or 28 nm.
  • the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .
  • a method and apparatus for generating electromagnetic waves are conventional techniques in the art.
  • the present invention provides the use of the above-described multi-pole micro-kinetic energy drinking water in the preparation of various mineral water, purified water, beverages, health care products or medicines.
  • the beverage is a functional beverage and can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea.
  • the health care product or drug can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid lowering and blood urea lowering.
  • the invention adopts electromagnetic wave non-contact type treatment water, without adding any additives, is simple to manufacture, has high production efficiency and low cost.
  • the multi-pole micro-kinetic energy drinking water of the present invention is more than the drinking water before treatment.
  • the original water produced a significant shift in the ultraviolet absorption peak to the short-wave direction.
  • the spectral intensity of the fluorescent radiation was greatly improved in the 300-400 nm spectrum, and more extranuclear electrons were in the high-energy non-radiative energy level.
  • Animal experiments have proven to have anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea reduction.
  • FIG 1 shows the effect of water in different treatment groups on peroxide-induced DNA damage
  • FIG. 1 shows the effect of differently treated media on cells
  • 3 is an absorption spectrum distribution of the ordinary drinking water raw water and the multi-pole micro kinetic energy of the present invention obtained by using the ordinary drinking water raw water;
  • Figure 6 is a fluorescence steady state transient test result of ordinary drinking water (raw water) and its multi-pole micro-kinetic energy drinking water;
  • Figure 7 is a fluorescence steady state transient test result of Evian natural mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;
  • Figure 8 shows the fluorescence steady-state transient test results of Vietnamese glacial mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;
  • Figure 9 shows the results of repeatability test of three samples of multi-pole micro-kinetic energy drinking water made from raw water of Georgia glacial mineral water;
  • Figure 10 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on the small bowel peristalsis inhibition model in mice;
  • Figure 11 is a view showing the effect of the multi-polar micro-kinetic energy drinking water of the present invention on the first black stool defecation time in a mouse constipation model
  • Figure 12 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on the fecal weight of a mouse constipation model
  • Figure 13 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on the body weight of mice
  • Figure 14 is a view showing the effect of the multi-polar micro-kinetic energy drinking water of the present invention on the discharge time of the first black stool in the mouse constipation model;
  • Figure 15 is a view showing the effect of the multi-polar micro-kinetic energy drinking water on the defecation particles of the mouse constipation model of the present invention.
  • Figure 16 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on the defecation weight of a mouse constipation model of the present invention.
  • electromagnetic waves of three different frequencies of low, medium and high are applied in a non-contact manner, wherein the frequency of the low frequency electromagnetic wave is 30 kHz, the frequency range of the intermediate frequency electromagnetic wave is 550 kHz, and the frequency range of the high frequency electromagnetic wave is 300 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both sinusoidal waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 100 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 725 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • electromagnetic waves of low, medium and high frequencies are applied non-contactly, wherein the frequency of the low frequency electromagnetic waves is 45 kHz, the frequency range of the intermediate frequency electromagnetic waves is 600 kHz, and the frequency range of the high frequency electromagnetic waves is 300 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the two electromagnetic wave propagation sides Consistent.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 75 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 425 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are sharp waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • the multi-pole micro-kinetic energy drinking water prepared in Examples 1-4 was tested according to the Standard Test Method for Drinking Water (GB/T 5750-2006), and the test results were basically the same. The results are shown in Table 1.
  • the indicators of the micro-kinetic energy drinking water of the present invention all meet the requirements of the Sanitary Standard for Drinking Water (GB5749-2006).
  • Dosage grouping The test substance is set at a dose of 20,000 mg/kg body weight, and each of the female and male animals is 10. Grouped by sex. The dose was calculated by oral gavage at 0.1 ml/10 g body weight.
  • the oral LD 50 of the test article for both female and male mice is greater than 10000 mg/kg body weight, which is an actual non-toxic grade.
  • Plasmid DNA pET28a plasmid was amplified and extracted in DH5a E. coli (plasmid extraction kit was purchased from Promega);
  • Electrophoresis Instrument Liuyi Instrument Factory
  • Test substance Micro-kinetic energy drinking water prepared in Examples 3 and 4
  • Group I untreated group, adding untreated sterile water to the reaction system;
  • Group II the kinetic energy drinking water prepared in Example 3;
  • Group III The kinetic energy drinking water prepared in Example 4.
  • reaction materials were added in the following order, and the final volume of the reaction system was 10 ⁇ L:
  • reaction was stopped by adding 1 ⁇ L of 5 mM EDTA and 2.2 ⁇ L of loading buffer at 0.5 hour and 1 hour, respectively.
  • the redox reaction between vitamin C and divalent copper ions is accompanied by the formation of peroxide, which causes damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band.
  • the results of this experiment are shown in Figure 1.
  • the experiments were divided into three groups. The grouping method is described in the scheme. The two time points of each group were: 0.5 hours and 1 hour, and the brightness of DNA represented the DNA content.
  • the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no effect of preventing or accelerating DNA damage and cleavage due to water treatment.
  • the redox reaction between vitamin C and divalent copper ions is accompanied by the formation of peroxide, which causes damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band.
  • the results of this experiment showed that the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no industrial effect of preventing or accelerating DNA damage and cleavage due to water treatment. It is indicated that the multi-pole micro kinetic energy of the present invention has no effect on peroxide-induced DNA damage. ring.
  • the cell culture medium adherently cultured in a 96-well plate was changed to medium A and medium B containing FAM-C6-8 aptamer, cultured at 37 ° C for 30 min, washed once with PBS, and observed under a fluorescence microscope.
  • Fig. 2 There is no difference in cell morphology between the treated group and the untreated group.
  • the cells are white-fluorescent, the cell membrane is intact, and no obvious FAM-labeled C6-8aptamer green fluorescence is observed in the cells.
  • the FAM-labeled C6-8 aptamer can enter the cell and bind to the protein inside the cell, and emit green fluorescence after excitation at 530 wavelength.
  • the results in the figure show that there is no difference between the cells in the treated group and the untreated group, all of which are autofluorescence, indicating that the cell membrane is intact, and the multipolar microkinetic energy technology of the present invention has no effect on cell morphology and cell membrane integrity.
  • Example 7 Variation of broad spectral absorptivity and ultraviolet absorptivity of multi-pole micro-kinetic energy drinking water of the present invention
  • Glacier Mineral Water Purchase at Carrefour supermarket;
  • ultrapure water made by the German Sartorius ultrapure water preparation system
  • Example 3 The ordinary drinking water raw water used in Example 3 and the micro-kinetic energy drinking water prepared in Example 3 were placed in an experimental vessel, and the absorption of the spectral light spectrum of the standard light source of the sample of 200 nm to 800 nm was scanned, and the experimental results were recorded.
  • Example 3 The micro-kinetic energy prepared in Example 3 is placed in an ultraviolet intensity detecting system, the ultraviolet light source illuminates the water to be tested, and the photodetector Thorlab DET10A/M is read by an oscilloscope MS04104 to read ultraviolet rays that have not passed the water to be tested.
  • the intensity value is read by the value of the ultraviolet intensity of the water to be tested, continuously tested continuously for 72 hours, and the amount of change in ultraviolet intensity during the recording.
  • Example 3 The drinking water made from the Vietnamese glacial mineral water in the same manner as in Example 3 is placed in the ultraviolet intensity detecting system, and the step (2) is repeated to record the amount of change in the ultraviolet intensity during the recording.
  • Multi-pole micro-kinetic energy drinking water made from ordinary water
  • Multi-pole micro-kinetic energy drinking water made from Evian natural mineral water
  • Multi-pole micro-kinetic energy drinking water made from Vietnamese glacial mineral water
  • the absorption spectrum absorption peak of the multi-pole micro-kinetic energy drinking water of the present invention is shifted to a short wave by about 30 nm;
  • Glacier Mineral Water Purchase at Carrefour supermarket;
  • ultrapure water made by the German Sartorius ultrapure water preparation system
  • Test environment temperature 22 ° C, humidity 37%.
  • Example 3 The micro-kinetic energy prepared in Example 3 is placed in a sample cell of a fiber optic spectrometer, and an excitation pulse is given to the sample by a 266 nm pulsed ultraviolet laser, and the spectral spectrum of the sample is used to measure the emission spectrum of the sample. Fluorescence emission spectrum of the sample.
  • Example 3 The ordinary drinking water raw water used in Example 3 was placed in the fiber optic spectrometer sample cell, and step 2 was repeated to record the experimental data.
  • the fluorescence steady-state transient test results of ordinary drinking water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 6.
  • the fluorescence steady-state transient test results of Evian natural mineral water and its multi-pole micro-kinetic drinking water are shown in Figure 7.
  • the fluorescence steady-state transient test results of Vietnamese glacial mineral water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 8.
  • the glacial mineral water in Moscow was tested for repeatability by taking three samples of multi-polar micro-kinetic energy drinking water made of raw water. The results are shown in Figure 9.
  • Example 9 Effect on mouse model of constipation and defecation time model, defecation and stool weight inhibition of intestinal motility in mice
  • the multi-pole micro-kinetic energy drinking water (kinetic energy water) prepared in Example 3 is a colorless and odorless transparent aqueous liquid, which is stored at room temperature and used within 72 hours after preparation;
  • the positive drug ⁇ cup Tongruntong tea produced by Shantou City Cup Co., Ltd., batch number 20140206.
  • Feeding conditions Experimental animal facilities continue to maintain barrier environmental standards.
  • the control range of the main environmental indicators room temperature 20 ⁇ 26 ° C, the daily temperature difference ⁇ 4 ° C.
  • the relative humidity is 40 to 70%.
  • Minimum air exchange times 15 times / hour, light illumination: dark 12h: 12h.
  • the animals are housed in a polypropylene rat group cage.
  • the cage specifications are: 545*395*200mm 3 , 5 per box, and the feeding space meets the minimum space required for experimental animals in the national standard GB14925-2010 of the People's Republic of China. Provisions. All animals are managed by trained personnel. Replace the litter and cage once a week. Rat-specific feed is added daily for animal consumption, and the animal's diet is kept free during the whole feeding process.
  • Baoding appliances should be of reasonable structure, appropriate specifications, sturdy and durable, environmentally friendly and easy to operate. The mandatory restrictions on the animal's body should be reduced to a minimum without affecting the experiment.
  • Sample collection The operation of collecting samples for experimental animals in a safe and humane manner (minimizing the tension and discomfort caused to animals).
  • Each experiment was divided into 5 groups: blank control group, model control group, daily drinking of normal drinking water (normal daily drinking water 0.3mL/10g body weight); kinetic energy low dose group daily drinking kinetic energy (drinking bottle filling) Kinetic energy, changed once a day); kinetic energy high-dose group, in addition to daily basic kinetic energy water, daily kinetic energy water, the dose is 0.3mL/10g body weight; positive drug ⁇ cup Tongruntong tea group daily irrigation
  • the stomach was administered once, the dose was 3.3g/kg, and the dosage volume was 0.3mL/10g body weight (running tea 3 bags per day, equivalent to 6.6g, human body weight calculated as 60kg, equivalent to 0.11g/kg, mice to clinical Calculated by 30 times the amount of human, it is equivalent to 3.3 g/kg, the dosage volume is 30 mL/kg, and the drug preparation concentration is 0.11 g/mL). There were no fasting and water prohibition requirements during the experiment. Continuous administration for 10 days.
  • Intestinal exercise test Pre-experiment was carried out before the formal experiment to determine the dose of compound diphenoxylate (5 mg/kg body weight) which can significantly reduce the intestinal propulsion rate of mice compared with normal mice, but not too low. Formal experiment.
  • mice After 10 days of the test sample, each group of mice was fasted for 16 hours.
  • the model control group and the three dose groups were intragastrically administered with the corresponding compound diphenoxylate suspension, and the blank control group was orally administered with water.
  • the dose group was given ink containing the corresponding test sample (containing 5% activated carbon powder, 10% gum arabic), and the blank control and model control group were given ink irrigation.
  • stomach Immediately after 20 minutes, the animals were sacrificed by cervical vertebrae. The mesenteric membrane was opened by abdominal cavity. The intestines from the pylorus and the lower end to the ileocecal part were cut out and placed on a tray.
  • the small intestine was gently drawn into a straight line, and the length of the intestine was measured as the “total length of the small intestine”. From the pylorus to the ink front, the "ink advancement length”. Calculate the ink advance rate as follows:
  • Defecation time experiment Pre-experiment was carried out before the formal experiment to determine the dose of compound diphenoxylate (10 mg/kg) which can significantly prolong the first black stool time of mice compared with normal mice, but not more than 5-6 hours. Body weight), formal experiment with this dose.
  • mice After 10 days of the test sample, each group of mice was fasted for 16 hours.
  • the blank control group was given drinking water, the model control group and the three administration groups by intragastric administration of compound diphenoxylate. 0.5 hours after oral administration of 10 mg/kg of compound diphenoxylate, the blank control group and the model control group were intragastrically administered with ink, and the dose group was given ink containing the test sample, and the animals were kept in a single cage, and the normal drinking water was fed. . Starting from the ink filling, record the first blackout time of each animal, record the number of black granules and the weight within 5 hours.
  • the weight results were processed using SPSS statistical software and compared between the drug-administered group and the control group. According to the following method: Kolmogorov-Smimov method should be used for normal test, Levene median method for homogeneity test of variance, if P>0.05, one-way ANOVA method, if normal and variance The homogeneity test failed (P ⁇ 0.05), then a non-parametric Mann-Whitney test was required.
  • the positive drug Runtong tea group showed a significant increase in ink propulsion rate (0.53 ⁇ 0.10*, vs 0.81 ⁇ 0.14,%, *p ⁇ 0.05), indicating that the model was reliable.
  • the average defecation time of the first black stool in the low-dose and high-dose groups of kinetic energy was significantly shortened, indicating that kinetic energy It can promote the average defecation time of the first black stool in the mouse constipation model.
  • the average defecation time of the first black stool in the positive drug Runtong tea group (79 ⁇ 64*, vs 151 ⁇ 61, min, *p ⁇ 0.05) was significantly shortened, indicating that the positive drug Runtong tea kinetic energy can promote
  • the first constipation model of the mouse constipation model averaged the time of defecation.
  • the weight of black stools in the low-dose and high-dose groups of kinetic energy (0.136+0.063*, 0.113+0.083, vs 0.081 ⁇ 0.062, g, *p ⁇ 0.01) increased significantly, indicating that kinetic energy can Increase the weight of black stools within 5 hours.
  • the weight of the black stool (0.161 ⁇ 0.098*, vs 0.081 ⁇ 0.062, g, *p ⁇ 0.01) in the positive drug ⁇ Cup Tongruntong tea group increased significantly within 5 hours, indicating that the positive drug Runtong tea kinetic energy Water can increase the weight of black stools within 5 hours.
  • kinetic energy can promote the average defecation time of the first black stool in the mouse constipation model, and increase the number and weight of black stools within 5 hours.
  • the multi-pole micro-kinetic energy drinking water of the present invention has a laxative function under the experimental conditions.
  • Example 10 Effect on intestinal movement creep mouse model Mice constipation and defecation time model, defecation and stool weight impact inhibition
  • Multi-polar micro-kinetic energy drinking water prepared in Example 4, colorless and odorless transparent aqueous liquid, stored at room temperature, used within 72 hours after preparation;
  • Blank control group normal drinking water and free feeding
  • Model control group normal drinking water and free feeding
  • Micro-kinetic water group The multi-pole micro-kinetic energy drinking water prepared in Example 4 was given and freely fed.
  • mice were given corresponding drinking water for 10 days, they were fasted for 16 hours.
  • mice after fasting were measured.
  • the compound difphenolate was given to the model control group and the micro-kinetic water group at 10 mg/kg body weight, while the blank control group was given the corresponding volume of normal drinking water.
  • mice After 30 minutes of administration, each group of mice was subjected to ink filling to mark feces.
  • Table 11 Effect of multi-polar micro-kinetic energy drinking water of the present invention on mouse weight, individual mouse data:
  • the average body weight of the micro-kinetic water group (20.37 ⁇ 0.316 vs 21.11 ⁇ 0.315, 21.03 ⁇ 0.322 g) showed no significant change compared with the blank control group and the model control group, indicating Short-term drinking of kinetic energy had no effect on the body weight of C57bl/6 mice.
  • Table 13 Effect of multi-polar micro-kinetic energy drinking water of the present invention on the discharge time of the first black stool in the mouse constipation model, individual mouse data:
  • Table 15 Effect of multi-polar micro-kinetic energy drinking water of the present invention on defecation granules in a mouse constipation model, individual mouse data:
  • Table 17 Effect of multi-polar micro-kinetic energy drinking water of the present invention on the weight of defecation in a mouse constipation model, individual mouse data:
  • the average defecation weight of the model control group and the micro-kinetic water group was 5 hours (0.24 ⁇ 0.02 ### , 0.27 ⁇ 0.02 ### vs 0.43 ⁇ 0.01) compared with the blank control group. ; ### P ⁇ 0.001) significantly decreased; compared with the model control group, the mean defecation weight (0.27 ⁇ 0.02 vs 0.24 ⁇ 0.02) of the micro-kinetic water group increased slightly but no statistical difference.
  • the multi-polar micro-kinetic energy drinking water of the present invention can effectively shorten the discharge time of the first black stool of the mouse constipation model without changing the weight of the defecation particles and the defecation weight, and has a laxative function.

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Organic Chemistry (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne les utilisations d'une eau potable traitée à l'aide d'ondes électromagnétiques dans la préparation d'une boisson, d'un produit de soins de santé ou d'un médicament anti-constipation. L'eau potable est préparée par passage à travers une onde électromagnétique sans contact. Par comparaison avec l'eau potable brute, le pic d'absorption des ultraviolets est décalé de 25 à 40 nm en direction des ondes courtes.
PCT/CN2015/074758 2015-03-20 2015-03-20 Utilisations d'une eau potable traitée à l'aide d'ondes électromagnétiques dans la préparation d'une boisson, d'un produit de soins de santé ou d'un médicament anti-constipation WO2016149882A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/CN2015/074758 WO2016149882A1 (fr) 2015-03-20 2015-03-20 Utilisations d'une eau potable traitée à l'aide d'ondes électromagnétiques dans la préparation d'une boisson, d'un produit de soins de santé ou d'un médicament anti-constipation
AU2015387922A AU2015387922A1 (en) 2015-03-20 2015-03-20 Uses of electromagnetic wave treated drinking water in preparing drink, healthcare product or medicament used for loosening stool

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2015/074758 WO2016149882A1 (fr) 2015-03-20 2015-03-20 Utilisations d'une eau potable traitée à l'aide d'ondes électromagnétiques dans la préparation d'une boisson, d'un produit de soins de santé ou d'un médicament anti-constipation

Publications (1)

Publication Number Publication Date
WO2016149882A1 true WO2016149882A1 (fr) 2016-09-29

Family

ID=56976857

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2015/074758 WO2016149882A1 (fr) 2015-03-20 2015-03-20 Utilisations d'une eau potable traitée à l'aide d'ondes électromagnétiques dans la préparation d'une boisson, d'un produit de soins de santé ou d'un médicament anti-constipation

Country Status (2)

Country Link
AU (1) AU2015387922A1 (fr)
WO (1) WO2016149882A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101046686A (zh) * 2007-03-21 2007-10-03 王广生 智能化水处理装置
CN101466643A (zh) * 2006-05-29 2009-06-24 株式会社志贺机能水研究所 水的电磁场处理方法及电磁场处理装置
CN103058430A (zh) * 2012-12-18 2013-04-24 华南理工大学 一种高能小分子水的制备方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101466643A (zh) * 2006-05-29 2009-06-24 株式会社志贺机能水研究所 水的电磁场处理方法及电磁场处理装置
CN101046686A (zh) * 2007-03-21 2007-10-03 王广生 智能化水处理装置
CN103058430A (zh) * 2012-12-18 2013-04-24 华南理工大学 一种高能小分子水的制备方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LU , JIANG: "Changing Water Structure for Creative Health Life", DIET SCIENCE, 31 January 2002 (2002-01-31) *

Also Published As

Publication number Publication date
AU2015387922A1 (en) 2017-11-09

Similar Documents

Publication Publication Date Title
Wang et al. A polysaccharide from cultured mycelium of Hericium erinaceus relieves ulcerative colitis by counteracting oxidative stress and improving mitochondrial function
Jiang et al. Sodium hydrosulfide attenuates cerebral ischemia/reperfusion injury by suppressing overactivated autophagy in rats
JP6691609B2 (ja) 新規の組換え高安定性スーパーオキシドジスムターゼおよびその応用
Samarasinghe et al. The effect of oral administration of iron saturated-bovine lactoferrin encapsulated chitosan-nanocarriers on osteoarthritis
Xu et al. Arthritic microenvironment actuated nanomotors for active rheumatoid arthritis therapy
Thombre et al. Floating-bioadhesive gastroretentive Caesalpinia pulcherrima-based beads of amoxicillin trihydrate for Helicobacter pylori eradication
Mannelli et al. Effect of the SOD mimetic MnL4 on in vitro and in vivo oxaliplatin toxicity: possible aid in chemotherapy induced neuropathy
Xu et al. Microplastics induced inflammation and apoptosis via ferroptosis and the NF-κB pathway in carp
Dhanapal et al. Chitosan/poly (lactic acid)-coated piceatannol nanoparticles exert an in vitro apoptosis activity on liver, lung and breast cancer cell lines
Zhao et al. Effects of microplastic and engineered nanomaterials on inflammatory bowel disease: a review
Li et al. Reprogramming Macrophage Polarization, Depleting ROS by Astaxanthin and Thioketal‐Containing Polymers Delivering Rapamycin for Osteoarthritis Treatment
Li et al. Plant-derived exosomal nanoparticles: potential therapeutic for inflammatory bowel disease
CN104720058A (zh) 多极微动能饮用水在制备用于通便的饮料、保健品或药物中的用途
CN104784207A (zh) 多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途
Lo et al. Plant-Derived Extracellular Vesicles: A New Revolutionization of Modern Healthy Diets and Biomedical Applications
Li et al. The cellular labeling and pH-sensitive responsive-drug release of celastrol in cancer cells based on Cys-CdTe QDs
CN104757675A (zh) 多极微动能饮用水在制备用于降血尿酸的饮料、保健品或药物中的用途
CN104757673A (zh) 多极微动能饮用水在制备用于降血压的饮料、保健品或药物中的用途
Wang et al. Colon‐Targeted Release of Gel Microspheres Loaded with Antioxidative Fullerenol for Relieving Radiation‐Induced Colon Injury and Regulating Intestinal Flora
Guo et al. Poly (p-coumaric acid) nanoparticles alleviate temporomandibular joint osteoarthritis by inhibiting chondrocyte ferroptosis
Cheng et al. Acid-base transformative HADLA micelles alleviate colitis by restoring adaptive immunity and gut microbiome
WO2016149882A1 (fr) Utilisations d'une eau potable traitée à l'aide d'ondes électromagnétiques dans la préparation d'une boisson, d'un produit de soins de santé ou d'un médicament anti-constipation
Xu et al. Alpha-mangostin attenuates oxidative stress and inflammation in adjuvant-induced arthritic rats
WO2016149886A1 (fr) Utilisations d'eau potable microcinétique multipolaire dans la préparation de boissons, de produits de santé ou de médicaments destinés à être utilisés pour réduire le taux d'urée dans le sang
WO2016149885A1 (fr) Utilisations d'eau potable microcinétique multipolaire dans la préparation de boissons, de produits de santé ou de médicaments permettant de réduire le taux d'urée dans le sang

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15885821

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2015387922

Country of ref document: AU

Date of ref document: 20150320

Kind code of ref document: A

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 25/01/2018)

122 Ep: pct application non-entry in european phase

Ref document number: 15885821

Country of ref document: EP

Kind code of ref document: A1