WO2016140506A1

WO2016140506A1 - High throughput photobioreactor

Info

Publication number: WO2016140506A1
Application number: PCT/KR2016/002079
Authority: WO
Inventors: 정신화; 김희식; 조대현; 오희목; 허진아
Original assignee: 한국생명공학연구원; 정신화
Priority date: 2015-03-03
Filing date: 2016-03-02
Publication date: 2016-09-09

Abstract

The present invention provides a high throughput photobioreactor. The high throughput photobioreactor may comprise: a chamber; a plate which is installed on the inside of the chamber and has a plurality of wells mounted thereon; a plurality of light sources which are installed on the inside of the chamber so as to radiate light in the plate direction; a light quantity control means which is positioned on the upper section of the plate so as to vary the quantity of light radiated on the plurality of wells; and a temperature control unit for controlling the temperature of the plate.

Description

High throughput photobioreactor

The present invention relates to high throughput photobioreactors, and more particularly to high throughput photobioreactors capable of culturing photosynthetic microorganisms.

High oil prices such as depletion of petroleum and natural gas and instability of supply and demand systems are being created all over the world, and the use of fossil energy is becoming visible to protect ecosystems such as climate change and environmental destruction.

As a result, countries around the world are concentrating on the development of renewable energy as well as the efficiency of existing thermal power generation and eco-friendly inventory. Biological energy production technology using photosynthetic microorganisms is also in the spotlight.

Recently, research on the utilization of photosynthetic microorganisms has focused on the production of biofuels for transportation due to the increase in the price of grain resources and food resources due to biofuel production. Applied research such as system research is being conducted in large scale.

Photosynthetic microbes can grow using water, carbon dioxide, and sunlight, and can be cultured anywhere in the wilderness, on the coast, or in the sea, and thus do not compete with existing land crops in terms of land or space. Photosynthetic microorganisms accumulate a large amount of lipids (up to 70%) in living organisms depending on culture conditions, and produce more than 50-100 times more oil (lipid) per unit area than conventional edible crops such as soybeans. Very high. Biodiesel, which is produced from photosynthetic microorganisms such as microalgae, can reduce pollutants such as fine dust and sulfur compounds significantly compared to existing diesel fuels.

In order to efficiently produce such photosynthetic microorganisms, development of high-efficiency photobioreactors and high concentration culture technologies has been attempted, and methods of culturing photosynthetic microorganisms such as microalgae can be largely divided into outdoor culture methods and photobioreactors.

In the case of the outdoor culture method, for example, the form of a pond or a channel that circulates the medium through the outer ring is an example of a low installation cost and an operating cost, while it is difficult to cultivate a high concentration and contaminated by other microorganisms, thereby increasing the recovery cost of the photosynthetic product. There are disadvantages.

Therefore, it is possible to produce high value-added substances such as biofuels, medicines, health foods, feeds, etc. using photosynthetic microorganisms, and in particular, high concentration and mass culture technology of photosynthetic microorganisms is essential for the biological carbon dioxide immobilization process. The demand for bioreactors is increasing.

At present, the type of optical bioreactor in Korea Patent No. 10-0283026 developed in Korea is disclosed as an air-induced photobioreactor using a cylindrical inner conduit as a light emitter, and in Korean Patent Publication No. 10-2003-0018196 The light emitting turbine type photobiological reactor used as a light emitting body is known.

Separation of high-productivity microalgae and establishment of optimal production conditions with such a photobioreactor are essential for increasing biomass productivity, and microalgae are highly dependent on productivity of light quantity, temperature and carbon dioxide. Separation and optimal culture conditions for high-productivity microalgae should take these photosynthetic requirements into account, but controlling the conditions of light quantity, temperature and carbon dioxide is difficult in a laboratory environment.

Accordingly, there is a need for a photobioreactor capable of establishing optimal production conditions.

The present invention has been proposed in order to solve the conventional problems as described above, an object of the present invention is to provide a high-throughput photobiological reactor which establishes optimum production conditions by culturing microorganisms in various environments by establishing various wide range and temperature conditions. It's there.

In another aspect, the present invention provides a high-throughput photobiological reactor that can more accurately set the optimum conditions required for the production of microalgae, and can easily and simply adjust the light quantity and temperature, even if the skilled researchers are not skilled It aims to do it.

As a technical aspect for achieving the above object, the present invention, the chamber; and the plate is installed in the chamber, the plurality of wells are mounted; and is installed inside the chamber to irradiate light toward the plate And a light quantity adjusting means positioned at an upper portion of the plate to vary the amount of light irradiated to the plurality of wells, and a temperature adjusting unit for adjusting a temperature of the plate. Provide a reactor.

Preferably, the light source is provided in the chamber, and may be at least one of a light emitting diode (LED) and organic light emitting diodes (OLED).

Preferably, the light amount adjusting means may be a light amount adjusting film positioned between the plate and the light source and having a gradation.

Preferably, the light amount control film may be formed to have a gradual gradation to become transparent in achromatic color from one side to the other side in the longitudinal direction.

Preferably, the light amount control film may be formed to have a gradual gradation to become transparent in a chromatic color from one side to the other side in the longitudinal direction.

Preferably, the light amount adjusting means may be a dimmer connected to the light source to control the current supply amount to adjust the brightness of the light source.

Preferably, the plurality of light sources are formed in at least two groups so that the groups are spaced apart in the longitudinal direction, and the dimmers are connected to the light sources of the group, including at least two, and the amount of light according to each group. Can be adjusted differently.

Preferably, the temperature control unit is located in the lower portion of the plate, the hot water or heated air is injected, the first temperature control pipe for adjusting the temperature of the plate and cold water or cold air is injected to control the temperature of the plate And a temperature control block including a second temperature control pipe, and a temperature diffusion plate positioned between the temperature control block and the plate to maintain the temperature of the plate.

Preferably, the first temperature control pipe and the second temperature control pipe may be arranged spaced apart from each other in the lateral direction.

Preferably, the temperature control unit, a plurality of semiconductor devices extending in the longitudinal direction of the plate; and a temperature control device for controlling the temperature of the plate by heating or cooling the semiconductor device; The semiconductor devices may be spaced apart from each other in the lateral direction.

On the other hand, preferably, the optical bioreactor, a control unit for controlling the light quantity of the light amount adjusting means and the temperature of the temperature control unit; and receiving and visually displaying a signal relating to the temperature and the light quantity from the control unit, It may further include a monitoring unit for receiving a setting of the user for control.

Preferably, the temperature control unit, the first TEC module (Thermo-Electric Cooling module) that is heated or cooled to the temperature input by the control unit; and is installed to be spaced apart from the first TEC module, by the control unit A second TEC module having a higher temperature than that of the first TEC module; and an upper surface thereof is installed in surface contact with the lower surface of the plate, one lower surface of the second TEC module being in contact with the first TEC module, and the other lower surface thereof is the second TEC module. And a plate-shaped temperature gradient block in which both end portions are heated or cooled to different temperatures by the first TEC module and the second TEC module to form a temperature gradient. The plate may include the first TEC module. The temperature may be adjusted while generating a temperature gradient in the lateral direction by the temperature difference between the module and the second TEC module.

The temperature controller may further include a temperature sensor attached to the first TEC module and the second TEC module to sense a temperature.

Preferably, the control unit may finely increase or decrease the temperature of the first TEC module and the second TEC module by PWM (Pulse Width Modulation) control.

Preferably, the light source is provided in the chamber, the printed circuit board (PCB) having a predetermined circuit pattern, and the LED unit consisting of a plurality of LED elements disposed on the PCB and having the same amount of light in the longitudinal direction The LED substrate unit including a plurality of LED modules are arranged, wherein the light amount adjusting means, by the electronic control of the control unit, the plurality of LED units generates different amounts of light, the light gradient in the longitudinal direction to the LED module It can be made to form.

Preferably, the LED substrate may be detachably provided in the chamber so that the LED substrate may be replaced with various kinds of light sources.

According to the high-throughput photobioreactor according to one embodiment of the present invention, by culturing microorganisms in various environments by establishing a wide range of wide range and temperature conditions, it is possible to obtain the effect of establishing the optimum production conditions.

On the other hand, when using a high throughput photobioreactor according to another embodiment of the present invention, by providing a control unit connected to the control unit and the electronic control of temperature and light quantity, it is possible to more accurately set the optimum conditions for the production of microalgae And, even if you are not a skilled researcher can obtain an excellent effect that can easily and simply adjust the amount and temperature of light.

In addition, according to another embodiment of the present invention, since it is possible to easily set a wide range of temperature environment, it is possible to obtain an effect capable of culturing various kinds of microalgae efficiently.

In addition, according to another embodiment of the present invention, by providing a replaceable LED substrate, it is possible to apply the LED light suitable for the cultured photo organisms can further enhance the culture efficiency.

1 is a view showing a photobioreactor according to an embodiment of the present invention.

2 is an exploded perspective view of a photobioreactor according to an embodiment of the present invention.

3 is a block diagram of a photobioreactor according to an embodiment of the present invention.

4 is a view showing a light amount control means of the photobiological reactor according to an embodiment of the present invention.

5 is a view showing a temperature control unit of the photobioreactor according to an embodiment of the present invention.

6 is a view showing a temperature control unit of the photobioreactor according to an embodiment of the present invention.

7 is a photograph of a photobioreactor according to an embodiment of the present invention.

8 is a graph showing a temperature gradient and a light gradient of the photobioreactor according to an embodiment of the present invention.

9 is a graph showing the optimum growth conditions when culturing P.kessleri JD076 using the photobioreactor of the present invention ((A) absorbance value, (b) growth rate).

10 is a graph showing the optimal conditions of neutral lipid production when P.kessleri JD076 was cultured in an air feed culture using the photobioreactor of the present invention ((A) fluorescence intensity per unit cell (nile red intensity), ( B) neutral lipid productivity).

11 is a graph confirming the growth optimum conditions when the P.kessleri JD076 cultured under 5% CO2 supply ((A) absorbance value, (B) growth rate).

12 is a side view showing a photobioreactor according to another embodiment of the present invention.

13 is a front view showing a photobioreactor according to another embodiment of the present invention.

14 is a view of an example of a light source applied to another embodiment of the present invention as viewed from below.

15 is a view of the plate and the temperature control unit applied to another embodiment of the present invention seen from above.

16 is a side view of FIG. 15 viewed from the right side.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

Firstly, the embodiments described below are suitable embodiments for understanding the technical features of the present high throughput photobioreactor. However, the technical features of the present invention are not limited by the embodiments described or applied to the embodiments described below, and various modifications are possible within the technical scope of the present invention.

The present invention relates to a high throughput photobioreactor capable of culturing photosynthetic microorganisms in a variety of environments by establishing various broad range and temperature conditions in one culture.

In the present invention, photosynthetic microorganism means green algae, red algae, cyanobacteria capable of photosynthesis, for example, chlorella, Chlamydomanas, Haematococous, Botryococcus, Sene Scenedesmus, Spirulina, Tetraselmis, Dunaliella, and the like, but are not limited thereto. At this time, the microalgae can produce carotenoids, cells, phycobiliproteins, lipids, carbohydrates, unsaturated fatty acids, and proteins in culture vessels.

1 to 11 will be described with respect to the photobioreactor 100 according to an embodiment of the present invention.

1 to 7, the high throughput photobioreactor 100 according to the present invention is installed in a chamber 101, the chamber 101, and a plate 110 on which a plurality of wells 111 are mounted . Is installed in the chamber 101, the light source 120 for irradiating light toward the plate 110, provided between the plate 110 and the light source 120 is irradiated to the plurality of wells 111 It comprises a light quantity adjusting means 130 for varying the amount of light and a temperature control unit 200 for adjusting the temperature of the plate 110.

First, the chamber 101 is a conventional one formed with a receiving space of a predetermined size therein, a door that can open and close the interior may be installed. The plate 110 may be mounted in the chamber 101, and the light source 120, the light amount adjusting unit 130, and the temperature adjusting unit 200 may be mounted in the chamber 101.

In addition, the plate 110 may be equipped with a plurality of wells 111 in one frame, and the plurality of wells 111 may be aligned to efficiently detect various types of information in each well 111. Referring to FIG. 1, an example in which 96 wells 111 are mounted is illustrated, but four, eight, sixteen, and twenty-four wells 111 may be used according to a desired culture purpose.

In this case, the well 111 may be any shape such as a square column, a cylinder, a rhombus column, and a test tube shape as long as it can contain a liquid. The well 111 may be in the form of a square column or a cylinder having a flat bottom surface for optical detection.

In addition, the light required in the process of growing photosynthetic microorganisms may be irradiated from the light source 120 installed in the upper portion of the chamber 101. In this case, the light source 120 may be a light emitting diode (LED) or organic light emitting diodes (OLED). In particular, the driving of the light sources 120 may use one kind of light sources 120 or two or more kinds of light sources 120 in combination.

In addition, the photobioreactor 100 of the present invention can build a wide range of a single culture, which is provided by the light amount control film 131 between the plate 110 and the light source 120, each well The amount of light transmitted to 111 may be adjusted differently.

More specifically, the light amount control film 131 may be formed to have a gradation in transparency or color from one side to the other side, for example, may be formed to have a gradation that is gradually transparent in achromatic color from one side to the other side. In addition, it may be formed to have a gradation of transparent color gradually from dark gray to one side from the other side. In another embodiment, the layer may be formed to have a gradation that becomes transparent in color from one side to the other side.

Here, the term achromatic color refers to a generic term for an uncolored object color ranging from white to gray to black color, and chromic color refers to a color having a color among objects. The fluid color may be red, green, blue purple, or the like.

For example, it may be formed to have a gradation of a transparent color gradually from red to the other side from one side, and may be formed to have a gradation of a transparent color gradually from blue.

The light amount control film 130 may be a film printed to produce a gradation effect using a specific paint.

As a specific aspect, referring to FIG. 4, the light amount adjusting means 130 may be a dimmer 132 for differently adjusting the light amount of the light source 120. More specifically, the plurality of light sources 110 may be formed in at least two groups, and the groups may be spaced apart in the longitudinal direction, and the dimmers 132 may include at least two light sources 120 of the group. The amount of light can be adjusted differently according to each group. For example, it may be composed of eight groups, and the light amount of the eight groups may be adjusted to be different from each other.

That is, the brightness of the light source may be adjusted by controlling the supply amount of current for each light source through the dimmer 132.

On the other hand, the temperature control unit 200 includes a temperature control block 220 and the temperature diffusion plate 210, the chamber 101 using the temperature diffusion plate 210 and the temperature control block 220 for temperature control. It can maintain a constant temperature inside.

In particular, the temperature control block 220 may be composed of a first temperature control pipe 221 and a second temperature control pipe 222. That is, it is composed of a water circulation system including a plurality of circulation tubes, by circulating the water at a set temperature, it is possible to variously adjust the temperature of the plate 110.

More specifically, the first temperature control pipe 221 is injected with hot water or heated air, it is possible to adjust the temperature of the plate 110, the second temperature control pipe 222 is injected with cold water or cold air to the The temperature of the plate 110 may be adjusted. In this case, hot water or heated air injected into the first temperature control pipe 221 may be 30 to 50 ° C., and cold water or cold air injected into the second temperature control pipe 222 may be 4 to 15 ° C.

In particular, the specific growth rate of the microorganism is greatly affected by the environment in which the microorganism grows, in particular by the culture temperature. The photobioreactor 100 is to be maintained at a temperature suitable for the growth and production of microorganisms, the heat transfer phenomenon and temperature control in the reactor is an important factor in determining the characteristics and efficiency of the photobioreactor.

The photobioreactor 100 in the present invention is controlled by injecting hot water and cooling water using the temperature control block 220, the temperature of the culture solution by the temperature diffusion plate 210 can be maintained substantially constant.

Meanwhile, referring to FIG. 6, the temperature controller 200 may be formed of a semiconductor device 231. More specifically, the temperature controller 200 may include a plurality of semiconductor devices 231 extending in a longitudinal direction. And a temperature controller (not shown) for controlling the temperature of the plate 110 by heating or cooling the semiconductor device 231, wherein the semiconductor device 231 is a temperature control block 220. Similarly, the temperature can be controlled by being spaced apart from each other in the lateral direction.

In addition, the photobioreactor 100 of the present invention may further include a gas supply unit for supplying carbon dioxide into the chamber 101, wherein the gas supply unit supplies a supply pipe connected to the chamber 101 and the supply unit. Is installed on one side of the pipe may include a supply pump for pumping to supply carbon dioxide into the chamber (101).

In addition, an additional detection method may be performed in the photobioreactor 100 of the present invention in order to detect various types of photosynthetic microorganisms cultured in the well 111 at the bottom of each well 111. For example, dissolved oxygen, carbon dioxide, pH, etc. in the well 111 may be monitored through additional detection methods.

Hereinafter, an experimental example will be described in detail to help the understanding of the present invention. However, the following experimental examples are merely to illustrate the content of the present invention is not limited to the scope of the present invention. Experimental examples of the present invention are provided to more completely explain the present invention to those skilled in the art.

<실험예>Experimental Example

실험예 1. 광합성 미생물 및 배양 조건Experimental Example 1. Photosynthetic microorganisms and culture conditions

Parachlorella sp. (JD076) strain was used for photosynthetic microbial culture using the photobioreactor 100 in this experimental example, and BG11 medium was used. BG11 medium was prepared by mixing Stock No. 1 to Stock No. 9 disclosed in Table 1. At this time, K ₂ HPO ₄ , ammonium ferric citrate and trace metal solution were sterilized separately and added after sterilization of the medium. In particular, trace metal solutions include H ₃ BO ₃ , MnCl ₂ 4H ₂ O, ZnSO ₄ 7H ₂ O, Na ₂ MoO ₄ 2H ₂ O, CuSO ₄ 5H ₂ O and Co (NO ₃ ) as shown in Table 1 below. ₂ · 6H ₂ O was mixed and 1 ml per 1 L was diluted and used.

Stock No.Stock No.	배지성분Medium	용량Volume
1One	NaNO₃ NaNO ₃	15 g/L15 g / L
22	K₂HPO₄ K ₂ HPO ₄	0.04 g/L0.04 g / L
33	MgSO₄·7H₂OMgSO _{4 7} H ₂ O	0.075 g/L0.075 g / L
44	NaCO₃ NaCO ₃	0.0202 g/L0.0202 g / L
55	CaCl₂·2H₂OCaCl ₂ · 2H ₂ O	0.006 g/L0.006 g / L
66	Citric acidCitric acid	0.006 g/L0.006 g / L
77	Ammonium ferric citrateAmmonium ferric citrate	0.006 g/L0.006 g / L
88	Na₂·EDTANa ₂ · EDTA	0.001 g/L0.001 g / L
99	Trace metal solutionTrace metal solution
	H₃BO₃ H ₃ BO ₃	2.86 g/L2.86 g / L
	MnCl₂4H₂OMnCl ₂ 4H ₂ O	1.81 g/L1.81 g / L
	ZnSO₄·7H₂OZnSO ₄ · 7H ₂ O	0.22 g/L0.22 g / L
	Na₂MoO₄·2H₂ONa ₂ MoO ₄ 2H ₂ O	0.39 g/L0.39 g / L
	CuSO₄·5H₂OCuSO ₄ · 5H ₂ O	0.08 g/L0.08 g / L
	Co(NO₃)₂·6H₂OCo (NO ₃ ) ₂ 6H ₂ O	0.05 g/L0.05 g / L

In this case, a light source was used as an LED lamp, and the light quantity was measured using a Li-COR (Li-198) light meter (Li-COR, USA), and the temperature was measured using an IR thermometer (FLUKE, USE). The temperature of was measured. The measurement was repeated three times to give an average value, and the temperature gradient and light gradient occurring in each well are shown in FIG. 8. The temperature was 15 to 33 ° C., and the light was generated in the range of 4 to 450 μmol / m ² / s.

Further, gave cell density (OD) was filled with 200㎕ in the same manner in 0.1-well, culturing is in a medium that is not injected with CO ₂ 2 days the culture was incubated 2 days after the injection of 5% CO _2. After cultivation, the optical density was measured using a microplate absorbance reader (Tecan, Switzerland) to measure cell biomass and a microplate fluorescence reader (Biotek, USA). Was measured.

실험예 2. 광합성 미생물의 성장 최적 조건Experimental Example 2. Optimal Growth Conditions for Photosynthetic Microorganisms

Parachlorella kessleri (parachlorella kessleri. JD076) was cultured using the photobioreactor 100 in Experimental Example 1, and the absorbance, cell mass, and neutral lipid content after culture were measured in this Experimental Example.

9 is a graph showing the absorbance (A) and growth rate (B) when P.kessleri JD076 was cultured using the photobioreactor 100 of the present invention. Referring to FIG. 9, the biomass of parachlorella kessleri (JD076) increased up to 0.3 when no air supply (no CO ₂ was injected) to the photobioreactor 100 was performed. I could confirm it.

In particular, the optimum range is 29 ~ 30 ℃ temperature range in the section of the increase in the biomass, the light conditions showed the optimal conditions at 150 ~ 250 μmol / m ² / s. In particular, this result was analyzed in the same manner as the result of converting the absorbance value to the growth rate, it was confirmed that the maximum growth rate is 0.4 / day.

10 is a graph showing the optimal conditions of neutral lipid production when P.kessleri JD076 was cultured in an air feed culture using the photobioreactor 100 of the present invention ((A) fluorescence per unit cell, (B) neutral) Lipid productivity).

Referring to Figure 10 to determine the intracellular neutral lipid content through nile red (nile red) staining, as a result of the maximum storage of the neutral lipid in the cell is 24 ℃, the amount of light 280μmol / m ² / s conditions I could confirm it.

FIG. 11 is a graph ((A) absorbance value and (B) growth rate) showing optimal conditions when P.kessleri JD076 was cultured under 5% CO ₂ .

Referring to Figure 11, in the culture environment supplied with 5% CO ₂ , the optimum culture period was 24 ~ 30 ℃, it was confirmed that the light conditions are 400μmol / m ² / s. Also, the absorbance value and growth rate of cell mass were higher when carbon dioxide was supplied than when carbon dioxide was not supplied. More specifically, the cell mass was 0.55, and the growth rate was confirmed to be 0.8 / day.

That is, as a result of culturing P.kessleri JD076 using the photobioreactor 100 of the present invention, it was possible to confirm the change of the biomass according to temperature, light and CO ₂ in 4 days. Lipid content analysis through nile red staining could confirm the optimal culture conditions of microorganisms.

Meanwhile, referring to FIGS. 12 to 16, a high throughput photobioreactor 100 according to another embodiment of the present invention will be described.

The high throughput photobiological reactor 100 according to another embodiment shown in FIGS. 12 to 16 has a light source 520, a light quantity adjusting means, and a temperature control as compared to the embodiment shown in FIGS. 1 to 11. There is a difference in the unit 600, there is a difference in that the vibration means 700 is added. Therefore, hereinafter, a detailed description of the same configuration as the above embodiment will be omitted.

As shown in FIG. 12, the high throughput photobioreactor 100 according to another embodiment of the present invention includes a control unit for controlling the light quantity of the light quantity adjusting means 130 and the temperature of the temperature control unit 600. 300 and a monitoring unit 400 for receiving a signal related to the temperature and the amount of light from the control unit 300 to visually display and receiving a user's setting for control of the control unit 300.

The control unit 300 may control the light amount of the light amount adjusting unit 130 and the temperature of the temperature control unit 600 to be adjusted to a predetermined value.

For example, the controller 300 may include a main board having a main control unit (hereinafter referred to as MCU) installed in the chamber 101. In addition, the light source 520, the light amount adjusting means 130, and the temperature controller 600 may be electrically connected to the MCU to adjust the temperature and the light amount by the MCU.

However, the control unit 300 is not limited to the illustrated embodiment and the above example, and if the light quantity adjusting means 130 and the temperature control unit 600 is electrically connected to control the temperature and the light quantity Various modifications are possible.

On the other hand, the monitoring unit 400 may receive a signal related to the temperature and the amount of light from the control unit 300 and visually display it, and receive a user's setting for control of the control unit 300.

For example, the monitoring unit 400 may be configured as a touch screen so that a user may easily check information and input information. In addition, the monitoring unit 400 is connected to the control unit 300 provided to the MCU, it is possible to check the information on the light quantity and temperature in real time and to easily set the appropriate temperature.

Specifically, the current temperature and the expected temperature by the setting may be displayed on the touch screen. In addition, the current intensity of the LED provided to the light source 520 may be checked and a desired intensity may be set. However, the monitoring unit 400 is not limited to the above-described embodiment, and various modifications are possible if the user can receive and display a signal from the control unit 300 and input the necessary information.

When using the high throughput optical bioreactor 100 according to another embodiment of the present invention, by providing a control unit 300 and a monitoring unit 400 connected to the control unit 300 to control the temperature and light amount electronically, microalgae It is possible to more accurately set the optimum conditions required for the production of, and even if you are not a skilled researcher can obtain an excellent effect that can easily and simply adjust the amount of light and temperature.

On the other hand, referring to the embodiment shown in Figure 12 and 13, the temperature control unit 600 applied to another embodiment of the present invention, the first TEC is heated or cooled to the temperature input by the control unit 300 Module 610 (Thermo-Electric Cooling module), the second TEC module 620 is installed to be spaced apart from the first TEC module 610, the temperature is higher than the first TEC module 610, the upper surface The lower surface of the plate 110 is installed in surface contact, one lower surface is in contact with the first TEC module 610, the other lower surface is in contact with the second TEC module 620, the first TEC module 610 and the first 2 The temperature gradient block 630 of the plate-shaped temperature gradient block 630 in which both ends are heated or cooled to a different temperature to form a temperature gradient, and the temperature of the first TEC module 610 and the second TEC module 620. It may include a control unit 300 to control the.

In addition, the temperature of the plate 110 may be adjusted while a temperature gradient is generated in the well 111 by a temperature difference between the first TEC module 610 and the second TEC module 620.

Specifically, the temperature gradient block 630 may be formed in a plate shape, the upper surface may be installed to be in surface contact with the entire surface of the lower surface of the plate 110, a portion of the lower surface of the first TEC module 610 and the first 2 may be installed in contact with the TEC module 620. In addition, the temperature gradient block 630 is made of a material having good thermal conductivity to transfer the temperature of the first TEC module 610 and the second TEC module 620 to the plate 110 to the well 111 of the plate 110. Can be heated or cooled.

In this case, the first TEC module 610 may be heated or cooled to a temperature received by the controller 300. In addition, the second TEC module 620 may be installed to be spaced apart from the first TEC module 610, and a temperature higher than that of the first TEC module 610 may be input by the controller 300. In addition, the chamber 101 may include a cooling fan 640 that may induce the flow of air when the first TEC module 610 and the second TEC module 620 are cooled, and an outlet of air, although not shown. Can be.

In addition, the temperature gradient block 630 has one lower surface contacting the first TEC module 610 and the other lower surface contacting the second TEC module 620 so that the first TEC module 610 and the second TEC module are in contact with each other. By 620, both ends may be heated or cooled to different temperatures, thereby forming a temperature gradient in the transverse direction.

That is, only the both ends of the temperature gradient block 630 is in contact with the first TEC module 610 and the second TEC module 620 so that one end is the same temperature as the first TEC module 610, the other end is made of 2 becomes the same temperature as the TEC module 620, the temperature gradient block 630 of the region corresponding to the space (see Fig. 13) between the first TEC module 610 and the second TEC module 620 in the transverse direction A temperature gradient can be formed.

Accordingly, the well 111 mounted on the plate 110 may be in surface contact with the temperature gradient block 630 to receive a temperature, thereby causing a temperature gradient in the lateral direction. Therefore, in the plurality of wells 111, various temperature conditions may be formed within a temperature range between the first TEC module 610 and the first TEC module 610.

TEC module applied to the temperature control unit 600 of another embodiment of the present invention, as shown in the embodiment, a plurality of thermoelectric elements are formed thinly arranged, the first TEC module 610 and the second TEC module 620 By arranging the spaces apart at appropriate intervals, in particular, the temperature setting in the low temperature region may be possible. Therefore, the photobioreactor 100 according to another embodiment of the present invention can easily set a wide range of temperature environment, it is possible to obtain the effect of culturing various kinds of microalgae.

The temperature controller 600 may further include

temperature sensors

651 and 652 attached to the first TEC module 610 and the second TEC module 620 to sense temperature.

The

temperature sensors

651 and 652 may be attached to the first TEC module 610 and the second TEC module 620, respectively, and detect and control the temperature of the first TEC module 610 and the second TEC module 620. A signal can be sent to 300. The controller 300 may transmit the temperature information received from the

temperature sensors

651 and 652 to the monitoring unit 400, and the user may check the current temperature in real time through the monitoring unit 400.

On the other hand, the control unit 300 may finely increase or decrease the temperature of the first TEC module and the second TEC module by PWM (Pulse Width Modulation) control.

That is, the controller 300 provided to the MCU converts the input temperature signal into a PWM signal and outputs the PWM signal, so that the temperature of the first TEC module and the second TEC module gradually increases or decreases (for example, 0.1 ° C./sec). You can do that.

Meanwhile, referring to the exemplary embodiments illustrated in FIGS. 13 and 14, the light source 520 is provided inside the chamber 101, and is disposed on a printed circuit board (PCB) on which a predetermined circuit pattern is formed and on the PCB. The LED unit 525 including the plurality of LED elements 527 having the same light amount may be configured as the LED substrate 521 including the LED modules 523 in which the plurality of LED units 525 are arranged in the longitudinal direction.

In addition, the light amount adjusting unit 130 may be configured such that a light gradient is formed in the LED module 523 in the longitudinal direction by generating a plurality of LED units 525 by the electronic control of the controller 300. .

That is, the light source 520 is provided to the LED substrate 521 including the LED element 527, the LED substrate 521 may include a PCB and the LED module 523. At this time, the LED module 523 may be arranged in the longitudinal direction of the LED unit 525 formed in the horizontal direction, the LED unit 525 may include a plurality of LED elements 527 disposed in the longitudinal direction. . The number of LED elements 527 may be provided to correspond to the number of wells 111 mounted on the plate 110.

In addition, the controller 300 may control the plurality of LED units 525 arranged in the longitudinal direction to have different amounts of light, and more preferably, the amount of light gradually increases from one side of the longitudinal direction to the other side. Can be controlled. Accordingly, the LED module 523 is capable of irradiating light of various sizes in the longitudinal direction to the wells 111 mounted on the plate 110 by forming a gradient of the amount of light in the longitudinal direction.

In addition, since the light amount adjusting unit 130 performs the control of the light amount through the electronic control of the MCU provided to the control unit 300, the light amount adjusting unit 130 can provide the desired amount of light more accurately and easily.

Meanwhile, the LED substrate 521 may be detachably provided in the chamber 101 so that the LED substrate 521 may be replaced with various kinds of light sources 520.

Specifically, as shown in FIGS. 12 and 13, the LED substrate 521 may be bolted by the fastening member 540 inside the cover 103 of the chamber 101. When the LED substrate 521 is replaced with another light source 520 (for example, an LED module 523 that emits a different color), the bolt of the fastening member 540 is removed and the other LED is removed. It can be assembled by replacing with the substrate portion 521.

13 and 14, one side of the LED substrate 521 may include a power connection terminal 545 that receives power and light quantity information from the controller 300. Here, the power connection terminal 545 may include a plurality of pins corresponding to the pins of the PCB circuit of the LED substrate 521, and a plurality of wires for transmitting a signal from the controller 300 may be detachably connected. . Accordingly, even when the LED board unit 521 is replaced with the other LED board unit 521, the same light quantity information can be received by the power connection terminal 545 from the controller 300.

Therefore, since the amount of light is controlled by the light amount adjusting means 130 even after replacing the LED substrate 521, it is possible to further increase the cultivation efficiency by replacing without limitation to the kind of suitable LED light of the photo organism to be cultured.

However, the method of installing the LED substrate 521 in the chamber is not limited to the illustrated embodiment, and various modifications are possible as long as the LED substrate 521 can be detachably assembled.

On the other hand, the control unit 300 may include a constant current IC for flowing a constant current by control from the outside to supply a constant current to the LED module 523.

That is, since the MCU provided to the controller 300 includes a constant current IC, it is possible to stably supply a constant current having a constant size to the plurality of LED elements 527 by external control.

Meanwhile, referring to the embodiments illustrated in FIGS. 15 and 16, the high throughput photobioreactor 100 according to another embodiment of the present invention is installed to surround the edge of the plate 110, and the plate is formed by its own weight. It may further include a well holder 115 for close contact with the 110 and the temperature gradient block 630.

That is, the well holder 115 may be formed in a shape corresponding to the shape of the plate 110 and may be formed in a frame shape having a hollow portion into which the edge of the plate 110 may be fitted. And, more preferably, it may be made of a metal having a large mass.

Accordingly, the plate 110 may be in close contact with the temperature gradient block 630 by the weight of the well holder 115. Therefore, the well holder 115 may provide an effect of efficiently transferring the temperature of the temperature gradient block 630 to the plate 110 by bringing the plate 110 into close contact with the temperature gradient block 630.

Meanwhile, referring to FIGS. 15 and 16, the photobioreactor 100 according to another embodiment of the present invention is coupled to the well holder 115, and the plate 110 is performed to perform the stirring operation in the well 111. Vibration means 700 for generating a vibration of) may be further included.

That is, since the edge of the plate 110 is fitted to the well holder 115, when the vibrating means 700 vibrates the well holder 115, the vibrating body is transmitted to the entire plate 110, and the optical organism accommodated in the well 111 is provided. Agitation of can be achieved. At this time, the vibration means 700 may be made of, for example, a vibration motor, but is not limited thereto, and various modifications may be made if the plate 110 may be vibrated.

More preferably, the vibrating means 700 may be installed in a plurality of spaced apart from the well holder 115. That is, in the illustrated embodiment, the case in which two up and down are installed in the well holder 115 is illustrated, but is not limited thereto, and three or more vibration motors may be arranged at regular intervals.

Accordingly, it is possible to provide the effect of transmitting the vibration evenly throughout the plate 110.

When using the high throughput photobioreactor according to another embodiment of the present invention, by providing a control unit for controlling the temperature and the amount of light electronically and a monitoring unit connected to the control unit, it is possible to more accurately set the optimal conditions for the production of microalgae Even if you are not an experienced researcher, you can easily and simply control the light quantity and temperature.

While the invention has been shown and described in connection with specific embodiments so far, it will be appreciated that the invention can be varied and modified without departing from the spirit or scope of the invention as set forth in the claims below. It will be appreciated that those skilled in the art can easily know.

Claims

chamber;

A plate installed inside the chamber and equipped with a plurality of wells;

A plurality of light sources installed inside the chamber to irradiate light toward the plate;

Light amount adjusting means positioned at an upper portion of the plate to change an amount of light irradiated to the plurality of wells; And,

A temperature controller for controlling the temperature of the plate;

High throughput photobioreactor comprising a.
The method of claim 1,

The light source is provided in the chamber, the high throughput optical bioreactor, characterized in that at least one of the light emitting diodes (LED) and organic light emitting diodes (OLED).
The method of claim 1, wherein the light amount adjusting means,

And a light quantity controlling film positioned between the plate and the light source and having a gradation.
The method of claim 3, wherein the light amount control film,

A high throughput photobioreactor, characterized in that it is formed to have a gradual gradation to become achromatic and transparent from one side to the other in the longitudinal direction.
The method of claim 3, wherein the light amount control film,

High-throughput photobioreactor characterized in that it is formed to have a gradual gradation to become transparent in the chromatic color from one side to the other side in the longitudinal direction.
The method of claim 1, wherein the light amount adjusting means,

And a dimmer connected to the light source to control the current supply to adjust the brightness of the light source.
The method of claim 6,

The plurality of light sources are formed in at least two groups so that the groups are spaced apart in the longitudinal direction,

The dimmer is connected to the light source of the group, including at least two, high throughput photobiological reactor, characterized in that to adjust the amount of light differently according to each group.
The method of claim 1, wherein the temperature control unit,

Located at the bottom of the plate, the hot water or heated air is injected, the first temperature control pipe for adjusting the temperature of the plate and cold water or cold air is injected to include a second temperature control pipe for controlling the temperature of the plate A temperature control block; And,

Located between the temperature control block and the plate, the temperature diffusion plate for maintaining the temperature of the plate;

High throughput photobioreactor comprising a.
The method of claim 8,

The first temperature control pipe and the second temperature control pipe, high throughput optical bioreactor, characterized in that spaced apart from each other disposed in the transverse direction.
The method of claim 1, wherein the temperature control unit,

A plurality of semiconductor devices extending in the longitudinal direction of the plate; And

A temperature controller for heating or cooling the semiconductor device to adjust the temperature of the plate;

Including, The plurality of semiconductor devices are high throughput photobiological reactor, characterized in that arranged in the lateral direction spaced apart from each other.
The method of claim 1, wherein the photobiological reactor,

A control unit controlling the light amount of the light amount adjusting means and the temperature of the temperature adjusting unit; And,

A monitoring unit for receiving a signal related to a temperature and an amount of light from the controller and visually displaying the signal, and receiving a user's setting to control the controller;

High throughput photobioreactor further comprising a.
The method of claim 11, wherein the temperature control unit,

A first TEC module (Thermo-Electric Cooling module) heated or cooled to a temperature input by the controller;

A second TEC module installed to be spaced apart from the first TEC module and having a temperature higher than that of the first TEC module by the controller; And,

An upper surface is installed in surface contact with the lower surface of the plate, one lower surface is in contact with the first TEC module, the other lower surface is in contact with the second TEC module, by the first TEC module and the second TEC module A plate-shaped temperature gradient block whose both ends are heated or cooled to different temperatures to form a temperature gradient;

Including, The plate, High throughput photobioreactor characterized in that the temperature is adjusted while generating a temperature gradient in the well in the transverse direction by the temperature difference between the first TEC module and the second TEC module.
The method of claim 12,

The temperature control unit, the high-throughput photobiological reactor further comprises; a temperature sensor attached to the first TEC module and the second TEC module for sensing a temperature.
The method of claim 12,

The control unit is a high throughput photobiological reactor, characterized in that for increasing or decreasing the temperature of the first TEC module and the second TEC module finely by PWM (Pulse Width Modulation) control.
The method of claim 11,

The light source is provided inside the chamber, and a plurality of LED units including a printed circuit board (PCB) having a predetermined circuit pattern formed thereon and a plurality of LED elements disposed on the PCB and having the same amount of light are arranged in the longitudinal direction. It consists of an LED substrate including an LED module,

The light quantity adjusting means is a high-throughput photobiological reactor, characterized in that by the electronic control of the control unit, the plurality of LED units to generate different amounts of light to form a light gradient in the longitudinal direction of the LED module.
The method of claim 15,

The LED substrate, high throughput optical bioreactor, characterized in that the detachably provided in the chamber to be replaced with various kinds of light sources.
The method of claim 15,

The control unit includes a high-throughput photobioreactor comprising a constant current IC for flowing a constant current by control from the outside to supply a constant current to the LED module.
The method of claim 12,

The photobioreactor is installed so as to surround the edge of the plate, the high-throughput photobiological reactor, characterized in that it further comprises; a well holder for contacting the plate and the temperature gradient block by its own weight.
The method of claim 18,

The optical bioreactor is coupled to the well holder, vibrating means for generating a vibration of the plate to perform a stirring operation in the well; high throughput optical bioreactor further comprising.
The method of claim 19,

The vibrating means is a high throughput optical bioreactor, characterized in that a plurality of spaced apart is installed in the well holder.
The photobioreactor according to any one of claims 1 to 20,

And a gas supply unit for supplying carbon dioxide to the chamber.
The method of claim 21, wherein the gas supply unit,

A supply pipe connected to the chamber; And,

A supply pump installed at one side of the supply pipe to pump carbon dioxide into the chamber;

High throughput photobioreactor comprising a.