WO2016101931A2

WO2016101931A2 - High potency collection and processing solution for abelmoschus manihot flower crude drug

Info

Publication number: WO2016101931A2
Application number: PCT/CN2016/072612
Authority: WO
Inventors: 董文佳; 李建波; 李文一; 杨苏睿; 王建树; 王亚伦
Original assignee: 王亚伦
Priority date: 2016-01-28
Filing date: 2016-01-28
Publication date: 2016-06-30
Also published as: WO2016101931A3

Abstract

The present invention relates to the field of modern Chinese traditional medicine development and relates to a high potency processing solution for Abelmoschus manihot flower crude drug. The present invention is a processing solution designed taking into account the biological characteristics of individual crude drug flower, which is absent in prior art. Viewed from standardization and practicality, the present invention is far beyond any modified technical solutions. No detached live flower can compare to the crude drug obtained from the embodiments, as the potency active substance contains no less than 24 types of bioflavonoids, 19 types more than embodiments of the prior art, and contains 36 types of active ingredients detected in tests. Frozen Abelmoschus manihot flowers are thawed, crushed, pulverized, and ground into slurry, then dried and dehydrated, and made into medicinal slices. The effect of the product is almost identical to the effect of the product made by directly using live flower in real time in the operation.

Description

High-priced okra peanut medicine harvesting and disposal plan

Technical field

The invention belongs to the field of modern Chinese medicine development, and relates to a high-efficiency price disposal scheme of okra peanut medicine.

Background technique

The okra described in the text of the present invention is encoded under the okra strip of the national standard "Chinese Plant Classification and Code" GB/T 14467-93 is 6000010003 [Abelmoschus manihot (Linn.) Medicus]. In the classics of traditional Chinese medicine, see the repertoire of 2075 pages 4252 and 4254 published in the Dictionary of Traditional Chinese Medicine published by Shanghai Science and Technology Press in 1995.

According to conventional and classic writings, okra flowers should belong to a traditional Chinese medicine. In the traditional Chinese medicine of flowers, from large flowers such as lotus flowers, small flowers such as sweet-scented osmanthus, there are many records of the most efficient treatment options, and common use programs, such as chrysanthemum, honeysuckle related practical literature. However, the biological characteristics of okra are far different from those of traditional Chinese medicines, which have been misunderstood in their most efficient price disposal schemes. The scheme is not only the most efficient treatment, but the lowest titer. The most typical disposal is as follows: the invention patent authorization notice number CN1048879C is written in the literature. Only the corolla using the okra flower is used to calculate the extract valence component and the extraction ratio of the crude drug. The valence of the patent after the disposal of the crude drug The content of ingredients is less than 30% of the original ecological drugs. A large domestic pharmaceutical enterprise group purchased the patent at a high price, and on this basis, the patent application was re-applied. According to its patent plan, the type of crude flavonoids used in the patent was only 20% of the reported in the conventional literature. . Another example:

Channel 7 of China Central Television, December 5, 2014, "Science and Technology Court" 20141205 special program, "Picking the Flower King by Peanuts", introduced with a 30-minute video, the collection of okra raw medicine flowers. The main points are: the choice of individual flowers, only the corolla is selected. The conditions for limiting the time of collecting flowers and collecting flowers are: first, the flowering time is limited, within 1.5 hours from 4 to 5 in the afternoon, and the second is the shape of the flower. It is the flower ovary that grows to the peanuts, the corolla separates the whole flower's organism tissue, and the third is to use the touch of the corolla and the corolla to get it, and use it to make the product. The fourth is to illustrate the collection and use of the okra flowers. It is a collection of years of research, the most advanced scientific and technological achievements obtained by planting thousands of acres of okra per year. In addition, the significance of the production of science and education films by CCTV is even more self-evident.

The common sense of plant biology tells us that the corolla effect as a plant flower is self-evident. As an ovary of a plant organ, the flower core is the core element for expressing the functional components of different plant flowers, almost in the collection and use of all Chinese medicine peanut drugs. It is an indispensable condition for selecting the raw medicinal flower tissue with ovary and stamen. The collection and use of the above-mentioned okra raw medicinal flowers violates the basic biological common sense. It seems to buy and praise the "scientific and technological achievements", and the effect is that the raw medicine products are taken, and there is no such thing as the medicine. Odor and mouthfeel, traits and mouthfeel such as cotton wool, the potency component is lost more than 80%.

The solution of the invention completely abandons the above-mentioned "scientific and technological achievements" scheme, and the high-price price of the raw medicine flower is quite original flower, and the potency component has no loss, the flavor is fragrant, and the taste is good.

Summary of the invention

The invention is directed to the treatment of the biological characteristics of the individual raw flower, and the prior art does not involve this. From the standard to the practical point of view, the invention is far from the technical improvement scheme, and the raw medicine obtained in the implementation is There is no isolated fresh flower ratio, and the potency active substance contains more than 24 types of bioflavonoids, 19 more than the actual examples in the background art, and 36 kinds of active ingredients are detected.

The invention provides a scheme for high-efficiency price collection, disposal and putting-in time limit of okra peanut medicine, which is characterized in that: the raw medicine for harvesting the target is selected from the limited time limit of the morphology and biological development of the okra, from the limitation of the harvesting operation. To ensure the stable potency of the crude drug; after the okra opens the flowering buds within 3.59 hours or after the opening of the okra flower, the okra flower biological tissue before the yellowing discoloration is the subject of selection and harvesting. The form of the target is the flower stalk. The flower bud, the corolla, the stamen, the ovary, and the pistil are closely combined into one biological tissue; when the individual flower is harvested separately, the flower stalk tissue from the base of the flower stalk and the base of the flower bud is disconnected from the body; When harvesting flowers, the flower branches are disconnected from the base of the best individual flower buds in the open state; the above-mentioned isolated okra peanuts are put into use within 28 hours under normal temperature conditions, and the above-mentioned isolated okra flowers are used. For crude medicine, when it is refrigerated, the time limit for putting it into use is less than 7 days.

The above-mentioned isolated okra peanut medicine is used for freezing treatment, and after thawing, the time limit for putting into use is within 9 hours.

The above-mentioned okra opens the flower buds and buds, which means that the stalks of the flowers of the flower buds develop from the closed form to the open form, and the yellow flower tissue state is present at 8-12 cm in front of the tip of the flower bud.

The above individual flower harvesting means that the individual flowers are separated from the branches and branches of the main branches or main branches of the okra ecological plant.

The above-mentioned flower collection is the branch of the main stem or main branch of the original eco-plant of the okra. The flowers are more than two, and the branches are taken to pick the okra flower. This is different from the ornamental flower cutting. Take, just close to the lower part of the open flower, and the lower part of it can not be separated; the best individual flower in the open state of the flower branch means that the individual flower has the largest opening of the crown, and its color must also be One hour before the yellowing discoloration.

The above-mentioned okra flower is open and yellow, which means that the corolla is closed for 1 hour before the color change. The yellowing is the fresh primary color of the flower, and the discoloration is the light brown or light cyan or light red of the corolla.

The use of raw medicine flowers disposed by the above scheme is to directly consume or directly make Chinese herbal medicine products.

The flower stalk tissue from the base of the flower stalk and the base of the flower bud is dissected from the body, and the selection of the detachment point in this limited biological tissue site is random, and the flower of the okra plant is caused by the difference of region and climate. The leaves and branches are in different state of occlusion, so they can only be used according to the convenience of specific operations. The specific scheme of breaking off can be used by cutting instruments. The fingers are separated.

The above-mentioned okra flower yellowing discoloration judgment, specific to the region, climate, growing season, full flowering period, in the choice of time, the difference is great, in practice is in the specific orientation of planting okra, a period of time, relative There are differences in the law, relying on a magnifying glass to observe the local, at that time, the observation of the okra flower, but the regional season shown in the video of the technical background "Technology Court", in the 2 hours before the flowering section, you can stop observing, because The time period from then on is the same as the specific flowering scheme described in the video, and does not make any sense to the present invention.

The okra peanut medicine disposed in the solution of the present invention has 36 kinds of active ingredients in the component processing after directly processing into a Chinese herbal medicine piece, wherein the bioflavonoid component reaches 24 kinds: 36 monomer compounds are separated therefrom, and the chemical is utilized. Methods and various spectral techniques ( ¹ H NMR, ¹³ C NMR, EI-MS, ESI-MS, HSQC, HMBC, ¹ H- ¹ HCOSY) identified the chemical structures of 30 compounds, namely: α-pineol, Stigmasterol, β-sitosterol, β-carotene, caffeic acid, 6-methoxy-7-hydroxycoumarin, hexadecanoic acid, 26 carbonic acid, quercetin, quercetin-3'-O - glucoside, hyperoside, quercetin-3-O-β-D-glucoside, cotton phlecein-3'-0-β-D-glucoside, 3-0-caffeoyl quinic acid, Myricetin, 5-hydroxy-3',4',7,8-tetramethoxyflavone, 3',5-dihydroxy-7,8-trimethoxyflavone, linseed, gallic acid, 3', 5-dihydroxy-7,8-dimethoxyflavone-4'-0-β-D-glucoside, 4',5,7,8 tetramethoxyflavone, myricetin-3-0-β- D-glucoside, quercetin-3-0-β-D-inoside, quercetin-3-0-β-D-rutinoside, 4'-methoxy-5,7-dihydroxy Flavonoids-[0-β-D xylose-(1→3)]-0-β-D glucoside, 4',7-dimethoxy-5,7-dihydroxyflavone-[-0-β- D-xylose-(1→2)]-O-β-D-glucoside, 3,5-di-0-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, 3 4-di-O-caffeoylquinic acid (29) and 5-hydroxy-4',7,8-trimethoxyflavone.

Example 1

Beijing Tongzhou planted 10 acres of okra, when mid-August, is the okra flower bloom period. A vice president of the China Technology Exchange of the Ministry of Science and Technology introduced a general manager of a pharmaceutical company Cao, on-site inspection of okra peanut drug disposal, and the inventor team accompanied two people on the ground. Beforehand, after a week of observation, it is known that in the current month's climate, the time of the local okra flower to turn yellow and discoloration every day is similar to the picking time mentioned in the background art, that is, 4-5 o'clock in the afternoon. The flowering state of the Tongzhou planting area is a variety of states after the flower buds. The next day, at 11 o'clock in the morning, General Manager Cao took the car and two people, two inventors team, rushed to the Tongzhou Okra planting site to separate the individual flowers, one is to choose the 30-hour autumn after the flower buds Sunflower, the second is to choose the flower that is open at 11 o'clock in the afternoon at 4 and 5 o'clock in the afternoon. The flower shape is the combination of flower stalk, flower bud, corolla, stamen, ovary and pistil. Tissue, without manual, mechanical force, force tearing and peeling can not separate the tissues. At the time of harvesting, the flower buds were separated from the individual flower by the hand above the flower stalk of the selected flower, and 300 individual flowers were collected. Then, it is sent to the laboratory of the Institute of Botany, Chinese Academy of Sciences, North Fourth Ring for testing and analysis. The content of the iconic bioflavonoids, hypericin, is more than 1.18%, which is 1 times higher than the national standard. Still more.

Example 2

The International Industrialization Base of the Chinese Academy of Agricultural Sciences, an okra that grows in the planting area of the okra, faces the autumn of the year, and there is still okra flowering. According to the recent 12 days of observation, the yellowing discoloration of the flower was in the morning of the next day. At 1100, it is more realistic that the climate is getting colder and faster. Many of the bud flowers on the okra branches are likely to suffer from cold damage. So I decided to break the flowers from the branches, so I decided to start at 8 am on Sunday. After harvesting, the branches of flowers are collected and stored at room temperature for storage at the biochemical reserve. A part of the refrigerated storage is taken, and a part of the refrigerator is frozen. The refrigerated treatment is put into use within 7 days, and after thawing by the freezing treatment, it is put into use for 3 hours. The above two uses are respectively for the refrigerated okra flower, the defrosted okra flower is subjected to chipping refining and drying and dehydration to obtain a piece of cake, and the product effect is almost the same as that of the product directly put into operation by the ecological flower.

Claims

The invention provides a scheme for high-efficiency price collection, disposal and putting-in time limit of okra peanut medicine, which is characterized in that: the raw medicine for harvesting the target is selected from the limited time limit of the morphology and biological development of the okra, from the limitation of the harvesting operation. To ensure the stable potency of the crude drug; after the okra opens the flowering buds within 3.59 hours or after the opening of the okra flower, the okra flower biological tissue before the yellowing discoloration is the subject of selection and harvesting. The form of the target is the flower stalk. The flower bud, the corolla, the stamen, the ovary, and the pistil are closely combined into one biological tissue; when the individual flower is harvested separately, the flower stalk tissue from the base of the flower stalk and the base of the flower bud is disconnected from the body; When harvesting flowers, the flower branches are disconnected from the base of the best individual flower buds in the open state; the above-mentioned isolated okra peanuts are put into use within 28 hours under normal temperature conditions, and the above-mentioned isolated okra flowers are used. For crude medicine, when it is refrigerated, the time limit for putting it into use is less than 7 days.
The isolated okra peanut medicine according to claim 1, wherein the time limit for putting it into use after thawing is less than 9 hours.
The okra opening flower buds and buds according to claim 1 means that the stalks of the flowers of the flower buds develop from a closed form to an open form, and a yellow flower tissue state is present at 8-12 cm in front of the apex of the bud.
The individual flower harvesting according to claim 1, wherein the individual flowers are separated from the branch branches of the main branch or the main branch of the okra ecological plant.
The above-mentioned flower collection of claim 1 is the branch of the main branch of the okra original ecological plant or the branches of the main branch, and the flowers are more than two, and the branch is taken to pick the okra flower. In the cutting of ornamental flowers, it is only close to the lower part of the open flower, and the lower part of the flower can not be separated; the best individual flower in the open state of the flower branch refers to the largest opening of the individual flower. The color must also be one hour before the yellowing discoloration.
The yellowing discoloration of the okra flower according to claim 1 refers to a state in which the corolla is closed for 1 hour before discoloration, and the yellowing is a decrease in the fresh primary color of the flower, and the discoloration is a pale brown or light cyan or light red in the corolla.
The use of the raw medicinal flower disposed by the above-mentioned solution according to claim 1 is a direct consumption or direct use of a Chinese medicinal material.