WO2016098965A1 - Antiseptic composition and use thereof - Google Patents

Abstract

The present invention relates to an antiseptic composition exhibiting antibacterial and antifungal activities without causing skin irritation. Wet wipes, cleaning agents for the human body, cleaning agents for household products, diapers, hygiene products, cosmetic products, sanitizers, and the like using the antiseptic composition of the present invention exhibit excellent antibacterial and antifungal activities without causing skin irritation, and the stability of the antiseptic composition is high.

Description

Antiseptic composition and use thereof

The present invention relates to antiseptic compositions and uses thereof.

In today's daily life, microorganisms such as bacteria and fungi are not only numerous but also widely distributed in the natural world such as soil, air, water, and seawater, and can be grown and reproduced at any time when growth conditions are suitable. Diseases, food corruption, housing, clothing, industrial products to the damage caused by microorganisms is a serious situation.

In order to prevent the occurrence of microorganisms in breeding diapers, wet wipes, hygiene products, cosmetics, detergents, disinfectant products, etc., research is being actively carried out, and chloromethylisothiazolinone (CMIT) ), 3-iodo-2-propynylbutylcarbamate (IPBC), Benzalkonium Chloride (BKC), PHMG (polyhexamethylene guanidine), PHMB (polyhexamethylene biguanide), paraben Paraben and alcohol are used as antibacterial and fungicides.

More specifically, most of the wet and adult wipes currently produced use CMIT and PHMB, and 3-iodine-2-propynylbutyl carbamate is mainly used as an auxiliary ingredient. However, these substances have strong skin irritation, and PHMG, PHMB and PGH are guadiin-based chemicals that cause acute lung injury syndrome caused by a humidifier cleaner, and 3-iodine-2-propynylbutyl. Carbamate is a socially-issued substance that is regulated for infant products by Japan's Ministry of Health, Labor and Welfare, and therefore, development of an antiseptic substance that can replace them is required.

Cetylpyridinium Chloride (CPC) is a quaternary ammonium cation used in mouthwashes, toothpastes, oral sprays, nasal sprays, etc., which has bactericidal effects against bacteria and microorganisms, and prevents plaque from teeth. It is known to reduce gingivitis (Source: www.wikipedia.com). In this regard, U.S. Patent No. 6,861,047 discloses toothpastes and mouthwashes containing CPC and sodium dihydroacetate, and Korean Patent Application No. 10-2001-0045337 discloses seed or cultivars of soybeans such as bean sprouts. Disclosed is a method for preventing rot of two vegetables by dipping, spraying or irrigation of CPC. In addition, International Application PCT / US2000 / 011425 discloses four components: CPC, behentrinium methosulfate and stearyl alcohol, quaterinium, and cetrimonium chloride. Disclosed are a textile hygiene product selected from the group of diapers, hygienic tissues, and wet tissue products. However, since CPC starts to freeze at about 15 when dissolved in purified water and recrystallization starts at about 13, CPC has a problem in commercialization when purified water is used as a solvent.

Thus, the present inventors conducted a study in order to overcome the problems of the preservatives currently used, as a result of using a mixture of cetylpyridinium chloride and sodium metabisulfite, excellent antibacterial and antifungal power, while increasing the durability, When the freezing point and the recrystallization temperature is low and used in the product, it was confirmed that the skin has a hypoallergenic effect, which is excellent in stability, and completed the present invention.

[Preceding technical literature]

[Patent Documents]

(Patent Document 1) US Patent Registration No. 6,861,047

(Patent Document 2) Korean Patent Application No. 10-2001-0045337

An object of the present invention is not irritating skin, harmless to the human body, having the same or more antiseptic force without including components such as CMIT, PHMB, PHMG, Paraben, which may be harmful to the human body, low freezing point and recrystallization temperature The present invention provides an antiseptic composition having improved product stability and a use thereof.

The present invention provides an antiseptic composition comprising sodium metabisulfite (SDS) and cetylpyridinium chloride (CPC) in a weight ratio of 1: 0.2 to 5.

The antiseptic composition may further include any one selected from the group consisting of sodium sulfite, hexylene glycol, surfactants, and combinations thereof.

The preservative composition is SDS and sodium sulfite in the case of sodium sulfite 1: 0.1 to 1.5 by weight, SDS and hexylene glycol in the weight ratio of 1: 0.1 to 1.5 for hexylene glycol, SDS and It may include a surfactant 1: 0.1 to 300.

The surfactant may be a ionic surfactant of carboxylate, sulfate ester salt, sulfonate salt or phosphate ester salt; Polyethylene glycol-based or polyhydric alcohol-based non-ionic surfactants; And amino acid-based or betaine-based amphoteric surfactants.

The antiseptic composition may be one in which the formulation is selected from an aqueous solution, an emulsion, a dispersion, a suspension and a useful solution.

According to another embodiment of the present invention, the present invention provides a composition for wet wipes comprising the antiseptic composition.

The wet wipes composition may include 0.01 wt% to 10 wt% of the antiseptic composition.

The composition for wet wipes may further include any one selected from the group consisting of glycerin, citric acid, a pH adjusting agent, and combinations thereof.

According to another embodiment of the present invention, the present invention provides a cosmetic composition comprising the antiseptic composition.

The cosmetic composition may include 0.1 wt% to 10 wt% of the antiseptic composition.

The cosmetic composition may further include any one selected from the group consisting of cyclopentasiloxane (cyclopentasiloxane), disodium ethane (EDTA-2NA), glycerin, butylene glycol and combinations thereof.

According to another embodiment of the present invention, the present invention provides a shampoo composition comprising the antiseptic composition.

The shampoo composition may include 0.1 wt% to 10 wt% of the antiseptic composition.

The shampoo composition may further comprise any one selected from the group consisting of disodium coco ampodiacetate, cocamidopropyl betaine, disodium laureth sulfosuccinate, decyl glucoside, and combinations thereof.

According to another embodiment of the present invention, the present invention provides a detergent composition comprising the antiseptic composition.

The detergent composition may include 0.1 wt% to 10 wt% of the antiseptic composition.

According to another embodiment of the present invention, the present invention provides a composition for fabric softener comprising the antiseptic composition.

The fabric softener composition may include 0.1 wt% to 10 wt% of the antiseptic composition.

According to another embodiment of the present invention, the present invention provides an industrial detergent composition comprising the antiseptic composition.

The industrial detergent composition may include 0.1 wt% to 10 wt% of the antiseptic composition.

Other specific details of embodiments of the present invention are included in the following detailed description.

In the present invention, the antiseptic composition has excellent antibacterial and antifungal activity and persistence, thus protecting the product and the user against microorganisms from external exposure environment, having low skin irritation, harmless to the human body, and treating the antiseptic composition to the product. Not only does not affect the physical and chemical properties of the product, but also has high physical stability such as low temperature stability, high temperature stability and pH stability of the antiseptic composition, which is useful for wet wipes, human cleaners, household cleaners, diapers, hygiene products, cosmetics and disinfectants. Can be used.

The present invention relates to an antiseptic composition comprising sodium metabisulfite (SDS) and cetylpyridinium chloride (CPC).

The antiseptic composition has a sterile or bactericidal effect on microorganisms, and includes both an antibacterial effect on bacteria and an antifungal effect on fungi.

The sodium metabisulfite (SDS) is used as an antioxidant and a reducing agent, and the composition of the present invention was confirmed to have excellent antibacterial and antifungal effects by mixing with CPC. In particular, SDS has a disadvantage that there is a sulfuric acid odor at less than pH 5.0, in the antiseptic composition of the present invention confirmed the effect of improving the physical stability and odor.

The antiseptic composition may include SDS and CPC in a weight ratio of 1: 0.2 to 5, preferably, in a weight ratio of 1: 0.2 to 0.25 or 1: 4 to 5. When it satisfies the weight ratio, it is preferable for stabilizing physical properties and improving antibacterial and antifungal properties of the antiseptic composition.

The antiseptic composition may include 0.001 to 20% by weight of sodium metabisulfite, 0.001 to 20% by weight of cetylpyridinium chloride, and a residual amount of purified water.

If the cetylpyridinium chloride is less than 0.001% by weight relative to the total weight of the antiseptic composition, the antimicrobial and antifungal activity of the target microorganism is reduced, and the antimicrobial agent does not have an antimicrobial spectrum. There may be the above, it is difficult to control the physical properties such as low temperature stability, high temperature stability and pH stability in the manufacturing, and it is not suitable for use because of compatibility with other compounds.

If the sodium metabisulfite is less than 0.001% by weight relative to the total weight of the antiseptic composition, the antimicrobial and antifungal activity of the target microorganism is reduced, and the antimicrobial spectrum has no antimicrobial spectrum. It is difficult to control physical properties such as low temperature stability, high temperature stability and pH stability at the time of manufacture, and it is not suitable for use because it is incompatible with other compounds.

The antiseptic composition may further include any one selected from the group consisting of sodium sulfite, hexylene glycol, surfactants, and combinations thereof. When further comprising the component, the mixing ratio of the SDS and CPC is improved to further improve the antimicrobial and antifungal power of the preservative composition, the freezing point and recrystallization temperature is lowered, and the pH is stabilized to help stabilize the product Excellent effect.

In the case of the sodium sulfite, SDS and sodium sulfite may be included in a weight ratio of 1: 0.1 to 1.5, preferably 0.5 to 1.5. When the content of sodium sulfite is less than 0.1 weight ratio, it is difficult to control the freezing point and recrystallization temperature of the preservative composition, which is a problem in stabilization of the product, and when it exceeds 1.5 weight ratio, it is difficult to control physical properties such as low temperature stability and high temperature stability of the product and stabilize pH. There is a problem.

In the case of hexylene glycol, SDS and hexylene glycol may be included in a weight ratio of 1: 0.1 to 1.5, preferably 0.5 to 1.5. When the content of sodium sulfite is less than 0.1 weight ratio, it is difficult to control the freezing point and recrystallization temperature of the preservative composition, which is a problem in stabilization of the product, and when it exceeds 1.5 weight ratio, it is difficult to control physical properties such as low temperature stability and high temperature stability of the product and pH. There is a problem with stabilization.

In the case of the surfactant, the weight ratio of the SDS and the surfactant may include 1: 0.1 to 300, preferably 1: 0.1 to 10. If the content of the surfactant is outside the above range, it is difficult to control the freezing point and recrystallization temperature of the preservative composition and difficult to stabilize the pH, there is a problem in the stabilization of the product.

The surfactant can be anionic, non-ionic or amphoteric. The anionic surfactant may be carboxylate, sulfate ester salt, sulfonate salt or phosphate ester salt, and the like, and may be in the form of sodium salt, calcium salt, ammonium salt, ethanolamine salt, and the like. It may be a polyethylene glycol-based or polyhydric alcohol, etc., the amphoteric surfactant may be an amino acid or betaine (betaine) and the like.

More specifically, the anionic surfactants include fatty acid salts, alkyl dicarboxylates, alcohol sulfate ester salts, higher alkyl sulfonates, higher alkyl disulfonates, sulfated fatty acids and fatty acid sulfonated higher fatty acid salts, and higher alkyl phosphoric acid. Ester salt etc. can be illustrated.

Examples of the amphoteric surfactant include betaine amphoteric surfactants, glycine amphoteric surfactants, alanine amphoteric surfactants, and sulfobetaine amphoteric surfactants.

Examples of the non-ionic surfactants include glycerin esters of higher fatty acids, glycol esters of higher fatty acids, pentaerythritol esters of higher fatty acids, sucrose esters of higher fatty acids, sorbitan and mannitanic ester higher alcohol condensates of higher fatty acids, Higher fatty acid condensates, higher fatty acid amide condensates, higher alkylamine condensates, higher alkyl mercaptan condensates, alkylphenol condensates, polypropylene oxide condensates, and any one selected from the group consisting of It is not limited to.

The antiseptic composition may be one in which the formulation is selected from aqueous solutions, emulsions, dispersions, suspensions and useful solutions.

The antiseptic composition may further include additives such as perfumes, vegetable extracts, pH adjusting agents, other additives, purified water, thickeners and antioxidants.

The antiseptic composition of the present invention is sodium metabisulfite (SDS) and cetylpyridinium chloride (Cetylpyridinium chloride (CPC)), optionally sodium sulfite (Sodium Sulfite), hexylene glycol (Hexylene Glycol), Any one selected from the group consisting of surfactants, additives, and combinations thereof may be prepared by mixing at room temperature, preferably, it is prepared by mixing at 25 ° C. or higher and 80 ° C. or lower, and sonicated to enhance mixing. Or stirring may be performed. In addition, purified water may be prepared by mixing a predetermined amount with these components and stirring or drying after stirring.

According to another embodiment of the present invention, the present invention relates to a composition for wet wipes comprising the antiseptic composition.

The wet wipes composition may include 0.01 wt% to 10 wt% of the antiseptic composition, preferably 0.1 to 1.5 wt%. When the content of the antiseptic composition is less than 0.01% by weight, the antibacterial and antifungal effect and persistence is lowered, and when the content exceeds 10% by weight, there is a problem of severe skin irritation or difficult to control physical properties.

The composition for wet wipes may further include any one selected from the group consisting of a pH adjusting agent, a moisturizing agent, a flavoring agent, and a combination thereof, and preferably, 0.03 to 0.05% by weight of a pH adjusting agent, 0.2 to 0.7% by weight of a moisturizing agent, and 0.01 to 0.01 perfume 0.2 wt%.

The moisturizing agent may be used in the field to which the present invention belongs, and examples thereof include glycerin, butylene glycol, natural extracts, skin components, and the like.

The pH adjusting agent may be used that is commonly used in the field of the present invention, and examples thereof include citric acid, sodium citrate, lactic acid, sodium hydroxide, potassium hydroxide, tromethamine, tee.

The fragrance may be used commonly used in the field to which the present invention belongs, and includes, for example, plant extracts.

The wet tissue composition may have a pH of 4.5 to 6.0, preferably pH of 5.0 to 5.8. In the case of the wet wipes composition comprising the antiseptic composition of the present invention does not occur sulfuric acid odor by SDS even in the above pH range, it is excellent in low temperature stability of CPC can be used stably without changing the physical properties when manufacturing wet wipes, low skin irritation Has an excellent effect.

According to another embodiment of the present invention, the present invention relates to a cosmetic composition comprising the antiseptic composition.

The cosmetic composition is 0.1% by weight of the antiseptic composition It may be included in 10% by weight, preferably 0.1% to 1.5% by weight. If the content of the antiseptic composition is less than 0.1% by weight, the antimicrobial and antifungal effect and persistence is lowered, and when the content of the antiseptic composition is greater than 10% by weight, skin irritation is severe or physical properties are difficult to control, thereby deteriorating physical stability of the cosmetic composition.

The cosmetic composition may further include any one selected from the group consisting of pH adjusters, moisturizers, perfumes, surfactants, other additives, purified water, and combinations thereof, depending on the formulation or physical properties of the cosmetic composition to be prepared, preferably 0.01% to 10% by weight of pH adjuster, 0.01% to 30% by weight of moisturizer, 0.01% to 3.0% by weight of perfume, 0.01% to 30% by weight of surfactant, and other additives and purified water. In the case of the cosmetic composition satisfying the above range, the antibacterial and antifungal effect of the present invention is excellent, but the stability of physical properties is excellent.

According to another embodiment of the present invention, the present invention relates to a shampoo composition comprising the antiseptic composition.

The shampoo composition is 0.1 wt% to the preservative composition 10 It may be included in the weight%, preferably 0.1 to 1.5% by weight. If the content of the antiseptic composition is less than 0.1% by weight, the antimicrobial and antifungal effect and persistence is lowered, if the content exceeds 10% by weight of the skin irritation is severe or difficult to control the shampoo properties.

The shampoo composition may further include any one selected from the group consisting of pH adjusters, moisturizers, fragrances, surfactants, other additives, purified water and combinations thereof. Preferably, 0.01% to 10.0% by weight of pH adjuster, 0.01% to 30% by weight of moisturizer, 0.01% to 3.0% by weight of perfume, 0.01% to 30% by weight of surfactant, and other additives and purified water may be included. . In the case of the cosmetic composition satisfying the above range, the antibacterial and antifungal effect of the present invention is excellent, but the stability of physical properties is excellent.

According to another embodiment of the present invention, the present invention relates to a composition for detergent comprising the antiseptic composition.

The detergent composition may include 0.1 wt% to 10 wt% of the antiseptic composition, preferably 0.1 wt% to 1.5 wt%. When the content of the antiseptic composition is less than 0.1% by weight, the antibacterial and antifungal effects and persistence is lowered, and when the content of the antiseptic composition exceeds 10% by weight, skin irritation is severe or physical property is difficult to control.

The detergent composition may further include any one selected from the group consisting of pH adjusters, moisturizers, fragrances, surfactants, antifoaming agents, other additives, purified water, and combinations thereof. Preferably 0.01 wt% to 10.0 wt% of pH adjuster, 0.01 wt% to 30 wt% of moisturizer, 0.01 wt% to 3.0 wt% of perfume, 0.01 wt% to 30 wt% of surfactant, 0.01 wt% to 1 wt% of antifoaming agent, Other additives and purified water. In the case of the detergent composition satisfying the above range, while excellent in the antibacterial and antifungal effect of the present invention, there is an effect excellent in the stability of physical properties.

According to another embodiment of the present invention, the present invention relates to a composition for fabric softener comprising the antiseptic composition.

The composition for the fabric softener is 0.1% by weight to the antiseptic composition It may comprise 10% by weight. When the content of the antiseptic composition is less than 0.1% by weight, the antimicrobial and antifungal effect and persistence are inferior, and when the content is greater than 10% by weight, skin irritation is severe or it is difficult to control properties of the fabric softener.

The fabric softener composition may further include any one selected from the group consisting of pH adjusters, moisturizers, flavoring agents, surfactants, antifoaming agents, other additives, purified water and combinations thereof. Preferably 0.01 wt% to 10.0 wt% of pH adjuster, 0.01 wt% to 30 wt% of moisturizer, 0.01 wt% to 3.0 wt% of perfume, 0.01 wt% to 30 wt% of surfactant, 0.01 wt% to 1 wt% of antifoaming agent, Other additives and purified water. In the case of the fabric softener composition satisfying the above range, the antibacterial and antifungal effect of the present invention is excellent, but the stability of physical properties is excellent.

According to another embodiment of the present invention, the present invention relates to an industrial detergent composition comprising the antiseptic composition.

The industrial detergent composition is 0.1% by weight of the antiseptic composition It may be included in 10% by weight, preferably from 0.1% to 5% by weight. When the content of the antiseptic composition is less than 0.1% by weight, the antimicrobial and antifungal effect and persistence is lowered, and when the content of the antiseptic composition exceeds 10% by weight, it is difficult to control the physical properties of the industrial cleaning composition.

The industrial detergent composition may further include any one selected from the group consisting of the pH adjusting agent, moisturizer, flavoring agent, surfactant, antifoaming agent, other additives, purified water, and combinations thereof. Preferably 0.01 wt% to 10.0 wt% of pH adjuster, 0.01 wt% to 30 wt% of moisturizer, 0.01 wt% to 3.0 wt% of perfume, 0.01 wt% to 30 wt% of surfactant, 0.01 wt% to 1 wt% of antifoaming agent, Other additives and purified water. Industrial cleaning composition satisfying the above range, while excellent in the antibacterial and antifungal effect of the present invention, there is an effect of excellent stability of physical properties.

According to another embodiment of the present invention, the present invention provides an article comprising the antiseptic composition, and examples of the article include a diaper, a sanitary ware, a cosmetic, a detergent, an industrial cleaner, a disinfectant, and a hygiene product. The hygiene article may be, for example, oral tissue, mouthwash, shampoo, soap, shower gel, and the like.

Preferably, the article comprises an antiseptic composition of the present invention in an amount of 0.1 wt% to 10 wt% based on the total weight of the article. If the antiseptic composition is less than 0.1% by weight relative to the total weight of the article, the antimicrobial and antifungal effects are insufficient, and when the antiseptic composition is more than 10% by weight, the physical properties of the product and skin irritation may be caused.

An article comprising the antiseptic composition of the present invention may be prepared according to a method of manufacturing an article in the field of the present invention, for example, prepared by mixing the antiseptic composition of the present invention as an additional component or preparing an article. In the last step, it can be prepared by the method of treating the preservative composition of the present invention in the article of manufacture.

As a method of treating the antiseptic composition to an article, all conventionally used methods may be used. For example, the antiseptic composition may be sprayed onto the article, the antiseptic composition is impregnated with the manufactured article, or at a predetermined stage of the manufactured article manufacturing process. It can process by the method of adding an antiseptic composition and mixing.

If the article is a cosmetic, it can be prepared by the method of adding the antiseptic composition of the present invention to a certain step of the manufacturing article manufacturing process and mixing using a method such as a stirrer.

In case the article is a detergent, in the case of the preparation of a liquid detergent, the antiseptic composition of the present invention may be added to a predetermined step of the manufacturing process of the manufactured article and mixed by using a method such as a stirrer, and may be prepared in a solid or powder state. In the case of the preparation of the detergent, the preservative composition of the present invention may be added to a predetermined step of the manufacturing process of the manufactured article, and mixed by using a method such as a stirrer, dried, or crushed after drying.

If the article is a diaper and feminine hygiene products in most cases is made in the form of fibers and can be prepared by the method of adding the impregnated composition of the present invention, impregnated, combined and dried in a certain step of the manufacturing article manufacturing process.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily practice the present invention. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention.

< Example > Preparation of Preservative Composition

Sodium Metabisulfite (SDS) and Cetylpyridinium chloride (CPC), respectively, were dissolved in an appropriate amount of purified water and mixed at room temperature to prepare 1 kg of the antiseptic composition of the present invention. . In this experiment, using ion-exchange resin was prepared using purified water managed to less than 3ppm calcium weight.

Table 1

	Sodium Metabisulfite (ppm)	Cetylpyridinium chloride (ppm)
Comparative Example 1	1000	0
Example 1	1000	200
Example 2	1000	250
Example 3	1000	500
Example 4	1000	2500
Example 5	1000	4000
Example 6	1000	5000
Comparative Example 2	0	1000

In addition, SDS, CPC, sodium sulfite and hexylene glycol were dissolved in a composition as shown in Table 2 in an appropriate amount of purified water, and mixed at room temperature to prepare 1 kg of the antiseptic composition of the present invention. . In this experiment, using ion-exchange resin was prepared using purified water managed to less than 3ppm calcium weight.

TABLE 2

[Weight ratio]	Example 7	Example 8	Example 9	Example 10
Sodium Metabisulfite	10	10	10	10
Cetylpyridinium chloride	40	40	40	40
Sodium sulfite	0	10	0	5
Hexylene glycol	0	0	10	5
Purified water	80	80	80	80

In addition, SDS, CPC, sodium sulfite (SS), hexylene glycol (Hexylene Glycol, HG) and various surfactants were dissolved in an appropriate amount of purified water in the composition shown in Table 3 below, and mixed at room temperature, 1 kg of the antiseptic composition of the invention was prepared. In this experiment, using ion-exchange resin was prepared using purified water managed to less than 3ppm calcium weight.

TABLE 3

[Weight ratio]	SDS	CPC	SS	HG	Surfactant, weight ratio	Purified water
Example 11	2	8	5	5	Sodium lauryl sulfate, 5	75
Example 12	2	8	5	5	Sodium xylenesulfonate, 5	75
Example 13	2	8	5	5	Ammonium laureth sulfate, 5	75
Example 14	2	8	5	5	Disodium laureth sulfosuccinate, 5	75
Example 15	2	8	5	5	Sodium lauroyl sarcosinate, 5	75
Example 16	2	8	5	5	Laures-7, 5	75
Example 17	2	8	5	5	Laureth-9, 5	75
Example 18	2	8	5	5	Coco-glucoside, 5	75
Example 19	2	8	5	5	Polysorbate 20, 5	75
Example 20	2	8	5	5	Polysorbate 60, 5	75
Example 21	2	8	5	5	Disodium coco ampodiacetate, 5	75
Example 22	2	8	5	5	Cocamidopropyl betaine, 5	75

< Production Example  1> Wet wipes containing the antiseptic composition of the present invention

Wet tissue compositions were prepared using the antiseptic compositions prepared in Examples 1 to 6 and Comparative Examples 1 and 2 of Table 1. The antiseptic composition shown in Table 1 was added to purified water and stirred, and additionally, flavors, extracts, and moisturizers were added in any order and stirred at room temperature.

As the fragrance, 0.01% by weight of citrus flavor, 0.1% by weight of extract of Machi, and 0.5% by weight of glycerin as a moisturizing agent were added. After adding the fragrance, extract and moisturizer, 0.05 wt% of citric acid, sodium citrate and other pH adjusting agents were added as a pH adjusting agent, and the final product was prepared by buffering the pH to 4.8 to 5.8.

The wet tissue composition was sprayed onto the fabric using a spray apparatus when the wet tissue fabric passed through the converting machine through the wet tissue manufacturing machine, and the wet tissue fabric was cut to meet the specifications to prepare a wet tissue.

< Production Example  2> containing the antiseptic composition of the present invention Cosmetics  Preparation of the composition

To the composition shown in Table 4, to prepare a cosmetic composition comprising the antiseptic compositions of Examples 1 to 6 and Comparative Examples 1 and 2.

Table 4

No.	Trade Name	INCI Name	Content (% by weight)
One	DC9040	Cyclopentasiloxane (and) Dimethicone Crosspolymer	One
2	SF0005Z	Cyclopentasiloxane	10
3	Cegesoft SB 45	Shea butter	1.5
4	Olive Oil	Olea Europaea (Olive) Fruit Oil	0.5
5	Myritol 312	Caprilic / Capric Trigliceride	2
6	Cegesoft C24W	Ethylhexyl Palmitate	3
7	Abil EM 90	Cetyl PEG / PPG-10 / 1 dimethicone	1.2
8	DI Water	Water	to 100
9	Niacineamide	Niacineamide	5
10	EDTA2Na	Disodium EDTA	0.02
11	Keltrol-F	Xanthan Gum	0.2
12	Glycerin	Glycerin	5
13	1.3 BG	1.3 BG	5
14	Sodium hyaluronate	Sodium hyaluronate	0.05
15	Antiseptic composition	Example 1-6, Comparative Example 1-2	1.0

Specifically, among the components described in Table 4 above, No. 9-11 was stirred for 10 minutes at 78 degreeC in 2500 rpm Disper, and No. After stirring 1 ~ 8 also at 78 ℃, they were mixed with each other for 15 minutes with a Homo mixer at 4000rpm, was stirred at 78 ℃. Thereafter, the mixture was slowly cooled, and the emulsion was prepared by uniformly adding the fragrance and the antiseptic composition prepared in Example at a temperature of 40 ° C. or lower.

< Production Example  3> Preparation of shampoo comprising the antiseptic composition of the present invention

To the composition shown in Table 5, to prepare a shampoo comprising the antiseptic compositions of Examples 1 to 6 and Comparative Examples 1 and 2.

Table 5

No.	Trade Name	INCI Name	Content (% by weight)
One	Water	Water	qs
2	MICONIUM PQ10 J-3000	Polyquaternium-10	0.25
3	EDTA-2NA	Disodium ID	0.10
5	Miconol-c2m	Disodium coco ampodiacetate	15.00
6	Mitain-CA (S)	Cocamidopropyl Betaine	7.00
7	Miconate-LES	Disodium laureth sulfosuccinate	7.00
8	Plantacare-2000UP	Decylglucoside	5.00
9	1,3-Butylene Glycol	Butylene Glycol	2.00
10	DOE-120	Fiji-120methylglucose dioleate	0.50
11	CROTHIX-PA- (MH)	Fiji-150 pentaerythritol tetrastearate	0.50
12	Phospholipid-EFA	Linoleamidopropyl sebum-dimonium chloride phosphate	1.00
13	Gluadin-WLM BENZ	Hydrolyzed Wheat Protein	0.30
14	D-Panthenol	Panthenol	0.20
15	XIAMETER® MEM-1870 EMULSION	Dimethicone	1.00
16	Sodium Xylenesulfonate	Sodium xylenesulfonate	0.50
17	Fragrance	Spices	1.20
18	Jojoba-oil	Jojoba seed oil	0.50
19	Purac HS-88	Lactic acid	0.30
20	Antiseptic composition	Example 1-6, Comparative Example 1-2	1.0

Specifically, 1 to 3 times were added sequentially and then stirred until it became transparent at 40 ° C., then 5 to 8 times were added sequentially and stirred until it was transparent. Thereafter, Nos. 9 to 11 were premixed at 50 ° C. and added to the main mixture, and were sequentially added from No. 12 while gradually cooling. Shampoos were prepared by buffering to pH 5-6.

< Preparation Example 4 > Containing the antiseptic composition of the present invention Laundry detergent  Produce

To the composition shown in Table 6, to prepare a laundry detergent comprising the antiseptic compositions of Examples 1 to 6 and Comparative Examples 1 and 2.

Table 6

No.	Trade Name	INCI Name	content(%)
One	Purified water	WATER	to 100
2	LAS	linear alkyl benzene sulfonate	12.000
3	DEA	Cocamide DEA	4.500
4	SLES	Sodium Laureth Sulfate	1.000
5	Glucopon-650 EC	Akylpolyglucocide	0.500
6	EDTA-4NA	Tetrasodium EDTA	0.100
7	Red 40	BLUE 1	0.000
8	Citric acid	Citric acid	0.010
9	Grapefruit extract	Citrus Paradisi (Grapefruit) Fruit Extract	0.010
10	Green Tea Extract	Camellia Sinensis Leaf Extrac	0.010
11	incense	Fragrance	0.100
12	DB-110A	Dimethicone	0.100
13	Antiseptic composition	Example 1-6, Comparative Example 1-2	1.0

< Production Example  5> comprising the antiseptic composition of the present invention Fabric softener  Produce

To the composition shown in Table 7 below, fabric softeners containing the antiseptic compositions of Examples 1 to 6 and Comparative Examples 1 and 2 were prepared.

TABLE 7

No.	Trade Name	INCI Name	content(%)
One	Purified water	WATER	to 100
2	EDTA-4NA	Tetrasodium EDTA	0.100
3	Hisofter MEQ-80	Dihydrogenated Palmoyl Hydroxyethylmonium Methosulfate	3.0000
4	HONEOL LAE-15	Laureth-15	0.7000
5	MEG	Ethylene Glycol	3.0000
6	Citric acid	Citric acid	0.020
7	NaCl	NaCl	0.1000
8	incense	Fragrance	0.5000
9	DB-110A	Dimethicone	0.1000
10	Antiseptic composition	Example 1-6, Comparative Example 1-2	1.0

< Production Example  6> Preparation of industrial degreaser comprising the antiseptic composition of the present invention

To the composition shown in Table 8, was prepared an industrial degreaser comprising the antiseptic compositions of Examples 1 to 6 and Comparative Examples 1 and 2.

Table 8

No.	Trade Name	content(%)
One	Water	To 100
2	Tetrasodium EDTA, 38%	10
3	Sodium metasilicate anhydrous	5
4	PEG-16 tallow ethyl ammonium ethosulfate	3
5	Alcohol Ethoxylate	4
6	Antiseptic composition, Example 1-6, Comparative Example 1-2	1.0

< Experimental Example  1> Confirm the antibacterial and antifungal effect of the antiseptic composition of the present invention

As an antimicrobial and antifungal test evaluation method of the preservative composition of the present invention, Escherichia coli ( Esherichia) which is a bacterium through Shaking Flask Method coli ), Staphylococcus aureus and Pseudomonas aeruginosa , 100 mL of nutrient broth, Aspergillus niger , In the case of candida albicans , strains incubated in 100 mL of Potato dextrose Broth were inoculated with platinum. The inoculated medium was incubated at 30 ° C. for 24 hours in a shaking incubator. 1.00% by weight of each of the antiseptic compositions of Examples 1 to 6 and Comparative Examples 1 and 2 were added to an aqueous solution of 0.9% NaCl solution without preservatives, and the same amount of purified water was added to the control, which contained 100 ml of each. 10 ⁶ CFU / ml Escherichia in Tube coli ), 10 ⁶ CFU / ml Staphylococcus aureus , 10 ⁶ CFU / ml Pseudomonas aeruginosa, 10 ⁵ CFU / ml fungi ( Aspergillus niger ) and Candida albicans (candida albicans ) Were each inoculated. Thereafter, 1 mL of each solution was taken, 0.9 mL of 0.9% NaCl solution was mixed, 1 mL was further added, and the mixture was added dropwise to petri-dish in three replicates by spreading smearing. Escherichia coli coli ), Staphylococcus aureus , Pseudomonas aeruginosa ) was incubated for 2 days at 35 ℃ incubator, Aspergillus niger and candida albicans incubated for 3 days at 25 ℃ incubator, counting the number of colonies and multiply by dilution factor 1mL The number of sugar bacteria was used, and the results are shown in Table 9 below.

Table 9

Elapsed date	Test strain	Inoculation number	Control	Comparative Example 1	Example 1	Example 2	Example 3	Example 4	Example 5	Example 6	Comparative Example 2
			Strain (CFU / ml)
Day 3	Escherichia coli	1.3 x 10 6	4	One	0	0	0	0	0	0	One
	Staphylococcus aureus	1.04 x 10 6	4	One	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	3.4 x 10 6	4	One	0	0	0	0	0	0	One
	Candida albicans	1.48 x 10 5	4	2	0	0	0	0	0	0	2
	Black fungus	1.9 x 10 5	4	2	0	0	0	0	0	0	One
Day 7	Escherichia coli	1.3 x 10 6	4	0	0	0	0	0	0	0	One
	Staphylococcus aureus	1.04 x 10 6	4	0	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	3.4 x 10 6	4	0	0	0	0	0	0	0	One
	Candida albicans	1.48 x 10 5	4	2	0	0	0	0	0	0	3
	Black fungus	1.9 x 10 5	4	2	0	0	0	0	0	0	One

0 = No Contamination (Sterile)

1 = Trace of contamination (1 ~ 9 Colonies per streak)

2 = Light contamination (10 ~ 99 Colonies per streak)

3 = Moderate contamination (Greater than 100 colonies but still distinguishable)

4 = Heavy contamination

As confirmed in Table 8, in the case of the antiseptic composition of the present invention (Examples 1 to 6) using a mixture of sodium metabisulfite and cetylpyridinium chloride is superior to the antiseptic composition using SDS or CPC alone The antimicrobial and antifungal effect has an effect, and especially in the case of the antiseptic composition of Examples 1 to 6 it was confirmed that there is an excellent antimicrobial and antifungal effect of at least 99.9% in various strains.

< Experimental Example  2> Confirm the effect of the wet tissue containing the antiseptic composition of the present invention

The antimicrobial effect of the wet tissue prepared in Preparation Example 1 was tested, and the results are shown in Table 10. As a control, a wet tissue was prepared using the wet tissue composition prepared according to Preparation Example 1 by adding the same amount of purified water instead of the antiseptic composition of the present invention.

Table 10

Elapsed date	Test strain	Inoculation number	Control	Comparative Example 1	Example 1	Example 2	Example 3	Example 4	Example 5	Example 6	Comparative Example 2
		Strain (CFU / ml)
Day 2	Escherichia coli	2.5 x 10 6	3	2	2	2	2	One	0	0	2
	Staphylococcus aureus	1.5 x 10 6	4	2	2	2	2	One	0	0	2
	Pseudomonas aeruginosa	3.1 x 10 6	3	2	2	2	2	One	0	0	3
	Candida albicans	1.4 x 10 5	4	One	One	One	One	One	0	0	2
	Black fungus	1.9 x 10 5	4	2	2	One	One	One	One	0	3
Day 3	Escherichia coli	2.5 x 10 6	4	2	0	0	0	0	0	0	2
	Staphylococcus aureus	1.5 x 10 6	3	One	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	3.1 x 10 6	3	One	0	0	0	0	0	0	One
	Candida albicans	1.4 x 10 5	4	One	0	0	0	0	0	0	2
	Black fungus	1.9 x 10 5	4	2	0	0	0	0	0	0	One
Day 7	Escherichia coli	2.5 x 10 6	4	One	0	0	0	0	0	0	One
	Staphylococcus aureus	1.5 x 10 6	4	0	0	0	0	0	0	0	0
	Pseudomonas aeruginosa	3.1 x 10 6	4	0	0	0	0	0	0	0	0
	Candida albicans	1.4 x 10 5	4	0	0	0	0	0	0	0	3
	Black fungus	1.9 x 10 5	4	3	0	0	0	0	0	0	One

0 = No Contamination (Sterile)

1 = Trace of contamination (1 ~ 9 Colonies per streak)

2 = Light contamination (10 ~ 99 Colonies per streak)

3 = Moderate contamination (Greater than 100 colonies but still distinguishable)

4 = Heavy contamination

As shown in Table 10, in the case of the antiseptic composition (Examples 1 to 6) of the present invention using sodium metabisulfite and cetylpyridinium chloride in combination, antibacterial and antiseptic properties are superior to those of SDS or CPC alone. There was an antifungal effect, in particular, in the case of the antiseptic composition of Examples 1 to 6 it was confirmed that there are excellent antibacterial and antifungal effect of at least 99.9% in all the various strains within 3 days. In particular, in Examples 5 to 6 it was confirmed that the excellent antibacterial and antifungal effect of at least 99.9% in all the various strains on the second day.

< Experimental Example  3> containing the antiseptic composition of the present invention Cosmetics  Confirm the effect of the composition

The antimicrobial and antifungal effects of the cosmetic composition (emulsion) prepared in Preparation Example 2 were confirmed by the following method. With the present invention antibacterial and antifungal capacity test Evaluation of antiseptic compositions of the E. coli 10 ⁴ CFU / ml to the target product, a cosmetic composition emulsion (emulsion) with the ASTM D2574 method (Escherichia coli ), 10 ⁴ CFU / ml Staphylococcus aureus , 10 ⁴ CFU / ml Pseudomonas aeroginosa ) were mixed and streaking, and the results were observed after incubation at room temperature for 3 days and 7 days, and streaking candida albicans and Aspergillus niger at 10 ⁵ CFU / ml. The results were observed after incubation at room temperature for the initial, 3 days and 7 days. A control was prepared according to Preparation Example 2 by adding the same amount of purified water instead of the antiseptic composition of the present invention. The results of the antimicrobial and antifungal effects are shown in Table 11.

Table 11

Elapsed date	Test strain	Inoculation number	Control	Comparative Example 1	Example 1	Example 2	Example 3	Example 4	Example 5	Example 6	Comparative Example 2
		Strain (CFU / ml)
Day 2	Escherichia coli	2.8 x 10 6	3	One	2	One	One	One	0	0	2
	Staphylococcus aureus	1.6 x 10 6	4	One	One	One	One	One	0	0	2
	Pseudomonas aeruginosa	2.1 x 10 6	3	One	One	One	One	One	0	0	3
	Candida albicans	1.6 x 10 5	4	One	One	One	One	One	0	0	2
	Black fungus	1.4 x 10 5	4	2	One	One	One	One	0	0	4
Day 3	Escherichia coli	2.8 x 10 6	4	3	0	0	0	0	0	0	2
	Staphylococcus aureus	1.6 x 10 6	4	2	0	0	0	0	0	0	2
	Pseudomonas aeruginosa	2.1 x 10 6	4	2	0	0	0	0	0	0	2
	Candida albicans	1.6 x 10 5	4	2	0	0	0	0	0	0	3
	Black fungus	1.4 x 10 5	4	3	0	0	0	0	0	0	2
Day 7	Escherichia coli	2.8 x 10 6	4	3	0	0	0	0	0	0	One
	Staphylococcus aureus	1.6 x 10 6	4	2	0	0	0	0	0	0	2
	Pseudomonas aeruginosa	2.1 x 10 6	4	3	0	0	0	0	0	0	One
	Candida albicans	1.6 x 10 5	4	3	0	0	0	0	0	0	3
	Black fungus	1.4 x 10 5	4	2	0	0	0	0	0	0	2

0 = No Contamination (Sterile)

1 = Trace of contamination (1 ~ 9 Colonies per streak)

2 = Light contamination (10 ~ 99 Colonies per streak)

3 = Moderate contamination (Greater than 100 colonies but still distinguishable)

4 = Heavy contamination

As shown in the results of Table 11, Examples 1 to 6 of the antiseptic composition of the invention was excellent in the killing rate of more than 99.9% of all bacteria, fungi in the third day, in the cosmetic composition comprising the antiseptic compositions of Comparative Examples 1 and 2 Insufficient antibacterial activity against bacteria and fungi was confirmed to be not completely killed even on day 7. In addition, in Comparative Example 1, where the content of cetylpyridinium chloride is relatively high, it was confirmed that the antibacterial and antifungal activity was relatively weaker. Therefore, in the case of the antiseptic composition of the present invention, it was confirmed that the SDS and CPC are not simply mixed, but also have an antibacterial and antifungal effect improvement in the weight ratio. In particular, in Examples 5 to 6 antifungal was killed on the second day.

< Experimental Example  4> Confirmation of the effect of the shampoo containing the antiseptic composition of the present invention

Antimicrobial and antifungal effects of the shampoo prepared in Preparation Example 3 were tested in the following manner. As an antimicrobial and antifungal test evaluation method of the antiseptic composition of the present invention, Escherichia coli of 10 ⁴ CFU / ml in a shampoo as a target through ASTM D2574 method coli ), 10 ⁴ CFU / ml Staphylococcus aureus , 10 ⁴ CFU / ml Pseudomonas aeroginosa , streaking and mixed incubation at room temperature for 1, 3, 7 days The antifungal 10 ⁵ CFU / ml Candida albicans and Black fungus Aspergillus niger were also streaking, and the results were observed after incubation at room temperature for 3 days and 7 days. A control (control) was prepared according to Preparation Example 3 by adding the same amount of purified water instead of the preservative composition of the present invention. The test results are shown in Table 12 below.

Table 12

Elapsed date	Test strain	Inoculation number	Control	Comparative Example 1	Example 1	Example 2	Example 3	Example 4	Example 5	Example 6	Comparative Example 2
		Strain (CFU / ml)
Day 2	Escherichia coli	2.8 x 10 6	3	One	0	0	0	One	One	One	2
	Staphylococcus aureus	1.8 x 10 6	4	One	0	0	0	One	One	One	One
	Pseudomonas aeruginosa	2.9 x 10 6	4	2	0	0	0	One	One	One	One
	Candida albicans	1.4 x 10 5	4	3	0	0	One	One	One	One	3
	Black fungus	1.9 x 10 5	4	2	0	0	One	One	One	One	One
Day 3	Escherichia coli	2.8 x 10 6	4	3	0	0	0	0	0	0	One
	Staphylococcus aureus	1.8 x 10 6	4	2	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	2.9 x 10 6	4	2	0	0	0	0	0	0	0
	Candida albicans	1.4 x 10 5	4	2	0	0	0	0	0	0	3
	Black fungus	1.9 x 10 5	4	3	0	0	0	0	0	0	One
Day 7	Escherichia coli	2.8 x 10 6	3	3	0	0	0	0	0	0	2
	Staphylococcus aureus	1.8 x 10 6	4	2	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	2.9 x 10 6	4	3	0	0	0	0	0	0	One
	Candida albicans	1.4 x 10 5	4	3	0	0	0	0	0	0	3
	Black fungus	1.9 x 10 5	4	2	0	0	0	0	0	0	One

0 = No Contamination (Sterile)

1 = Trace of contamination (1 ~ 9 Colonies per streak)

2 = Light contamination (10 ~ 99 Colonies per streak)

3 = Moderate contamination (Greater than 100 colonies but still distinguishable)

4 = Heavy contamination

As shown in Table 12, in the case of Xiangpu comprising the antiseptic compositions of Examples 1 to 6, all bacteria and fungi were excellent in killing rate of 99.9% or more on Day 3, but of Comparative Example 1 and Comparative Example 2 In the shampoo composition including the antiseptic composition, the antimicrobial activity against bacteria and fungi was insufficient, so that the bacteria and fungi were not completely killed even on the 7th day. Therefore, even in the shampoo, it was confirmed that when using a mixture of SDS and CPC of the present invention in a specific ratio, an antiseptic composition having better antibacterial and antifungal effects than when using only one component each. In particular, in Examples 1 to 2 it was confirmed that the antifungal kill on the second day.

< Experimental Example  5> Confirm the effect of the laundry detergent containing the antiseptic composition of the present invention

The antimicrobial and antifungal effect of the laundry detergent prepared in Preparation Example 4 was confirmed by the following method. As an antimicrobial and antifungal test evaluation method of the antiseptic composition of the present invention, Escherichia coli of 10 ⁶ CFU / ml in a detergent as a target through ASTM D2574 method coli ), 10 ⁶ CFU / ml Staphylococcus aureus and 10 ⁶ CFU / ml Pseudomonas aeroginosa were mixed and streaking, and the results were observed after incubation at room temperature for 3 days and 7 days. In addition, streaking candida albicans and Aspergillus niger at 10 ⁵ CFU / ml, and the results were observed after incubation at room temperature for 3 days and 7 days. A control was prepared according to Preparation Example 4 by adding the same amount of purified water instead of the preservative composition of the present invention, the test results are shown in Table 13.

Table 13

Elapsed date	Test strain	Inoculation number	Control	Comparative Example 1	Example 1	Example 2	Example 3	Example 4	Example 5	Example 6	Comparative Example 2
		Strain (CFU / ml)
Day 2	Escherichia coli	3.3 x 10 6	2	One	0	0	0	0	0	One	2
	Staphylococcus aureus	2.8 x 10 6	3	One	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	2.1 x 10 6	3	2	0	0	0	0	0	0	One
	Candida albicans	2.4 x 10 5	3	3	0	0	One	0	One	One	3
	Black fungus	3.2 x 10 5	4	2	0	0	One	One	One	One	One
Day 3	Escherichia coli	3.3 x 10 6	4	One	0	0	0	0	0	0	One
	Staphylococcus aureus	2.8 x 10 6	4	0	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	2.1 x 10 6	4	0	0	0	0	0	0	0	0
	Candida albicans	2.4 x 10 5	4	0	0	0	0	0	0	0	3
	Black fungus	3.2 x 10 5	4	3	0	0	0	0	0	0	One
Day 7	Escherichia coli	3.3 x 10 6	2	3	0	0	0	0	0	0	2
	Staphylococcus aureus	2.8 x 10 6	3	0	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	2.1 x 10 6	3	0	0	0	0	0	0	0	One
	Candida albicans	2.4 x 10 5	3	One	0	0	0	0	0	0	3
	Black fungus	3.2 x 10 5	2	3	0	0	0	0	0	0	One

0 = No Contamination (Sterile)

1 = Trace of contamination (1 ~ 9 Colonies per streak)

2 = Light contamination (10 ~ 99 Colonies per streak)

3 = Moderate contamination (Greater than 100 colonies but still distinguishable)

4 = Heavy contamination

As shown in Table 13, in the laundry detergent containing the antiseptic compositions of Examples 1 to 6 of the present invention, all bacteria and fungi were killed at 99.9% or more on the third day, but Comparative Examples 1 to 2 In the laundry detergent composition of the bacteria and fungi lacking antimicrobial activity was not completely killed even on day 7. Therefore, it was confirmed that even when used in a laundry detergent mixed with the SDS and CPC of the present invention at a specific ratio, it was possible to prepare an antiseptic composition having better antibacterial and antifungal effects than when only one component was used. In particular, Examples 1 to 2 confirmed that all fungi were killed even on the second day.

< Experimental Example  6> fiber comprising the antiseptic composition of the present invention Softener  Check the effect

The antimicrobial and antifungal effects of the fabric softener prepared in Preparation Example 5 were tested in the following manner. As an antimicrobial and antifungal test evaluation method of the antiseptic composition of the present invention, Escherichia coli of 10 ⁶ CFU / ml in a detergent as a target through ASTM D2574 method coli ), 10 ⁶ CFU / ml Staphylococcus aureus and 10 ⁶ CFU / ml Pseudomonas aeroginosa were mixed and streaking, and the results were observed after incubation at room temperature for 3 days and 7 days. In addition, streaking candida albicans and Aspergillus niger at 10 ⁵ CFU / ml, and the results were observed after incubation at room temperature for 3 days and 7 days. A control was prepared according to Preparation Example 5 by adding the same amount of purified water instead of the preservative composition of the present invention, the test results are shown in Table 14.

Table 14

Elapsed date	Test strain	Inoculation number	Control	Comparative Example 1	Example 1	Example 2	Example 3	Example 4	Example 5	Example 6	Comparative Example 2
		Strain (CFU / ml)
Day 2	Escherichia coli	2.5 x 10 6	2	2	0	0	0	0	0	One	2
	Staphylococcus aureus	2.1 x 10 6	2	2	0	0	0	0	0	0	2
	Pseudomonas aeruginosa	4.2 x 10 6	2	2	0	0	0	0	0	0	2
	Candida albicans	1.9 x 10 5	2	One	0	0	One	One	One	One	3
	Black fungus	3.5 x 10 5	4	2	0	0	One	One	One	One	One
Day 3	Escherichia coli	2.5 x 10 6	2	2	0	0	0	0	0	0	2
	Staphylococcus aureus	2.1 x 10 6	One	One	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	4.2 x 10 6	One	One	0	0	0	0	0	0	One
	Candida albicans	1.9 x 10 5	2	2	0	0	0	0	0	0	2
	Black fungus	3.5 x 10 5	One	One	0	0	0	0	0	0	One
Day 7	Escherichia coli	2.5 x 10 6	One	One	0	0	0	0	0	0	One
	Staphylococcus aureus	2.1 x 10 6	One	One	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	4.2 x 10 6	2	2	0	0	0	0	0	0	2
	Candida albicans	1.9 x 10 5	One	One	0	0	0	0	0	0	One
	Black fungus	3.5 x 10 5	One	One	0	0	0	0	0	0	One

0 = No Contamination (Sterile)

1 = Trace of contamination (1 ~ 9 Colonies per streak)

2 = Light contamination (10 ~ 99 Colonies per streak)

3 = Moderate contamination (Greater than 100 colonies but still distinguishable)

4 = Heavy contamination

As shown in Table 14, in the case of the fabric softener composition containing the antiseptic compositions of Examples 1 to 6 of the present invention, all bacteria and fungi were excellent in killing rate of 99.9% or more on the third day, but Comparative Examples 1 to In the fabric softener composition of 2, the antimicrobial activity against bacteria and fungi was insufficient, and thus did not die completely even on the 7th day. Therefore, it was confirmed that even when the fiber softener is used by mixing the SDS and CPC of the present invention at a specific ratio, it is possible to prepare an antiseptic composition having better antibacterial and antifungal effects than when only one component is used. In particular, in Examples 1 to 2 it was confirmed that all fungi are killed on the second day.

< Experimental Example  7> Preparation of industrial degreaser containing preservative composition of the present invention.

The antibacterial and antifungal effects of the industrial degreaser remover prepared in Preparation Example 6 were tested in the following manner. As an antimicrobial and antifungal test evaluation method of the antiseptic composition of the present invention, 10 ⁶ CFU / ml Escherichia coli , 10 ⁶ CFU / ml Staphylococcus aureus , 10 ⁶ CFU / ml Pseudomonas aeroginosa were mixed and streaking, respectively, and the results were observed after incubation at room temperature for the initial, 3 and 7 days, and 10 ⁵ CFU / ml of candida albicans and black mold The bacteria ( Aspergillus niger ) were also streaking, and the results were observed after incubation at room temperature for the initial, 1, 3, and 7 days. A control was used to prepare an industrial degreaser removed according to Preparation Example 6 by adding the same amount of purified water instead of the preservative composition of the present invention, the test results are shown in Table 15.

Table 15

Elapsed date	Test strain	Inoculation number	Control	Comparative Example 1	Example 1	Example 2	Example 3	Example 4	Example 5	Example 6	Comparative Example 2
		Strain (CFU / ml)
Day 2	Escherichia coli	3.5 x 10 6	3	3	0	One	0	0	0	0	2
	Staphylococcus aureus	2.3x 10 6	3	3	One	One	One	One	0	0	2
	Pseudomonas aeruginosa	4.1 x 10 6	3	2	0	One	0	0	0	0	2
	Candida albicans	1.9 x 10 5	3	3	One	One	One	One	0	0	3
	Black fungus	3.8 x 10 5	4	3	One	One	One	One	0	0	One
Day 3	Escherichia coli	3.5 x 10 6	3	2	0	0	0	0	0	0	2
	Staphylococcus aureus	2.3x 10 6	2	One	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	4.1 x 10 6	3	One	0	0	0	0	0	0	One
	Candida albicans	1.9 x 10 5	2	2	0	0	0	0	0	0	2
	Black fungus	3.8 x 10 5	2	One	0	0	0	0	0	0	One
Day 7	Escherichia coli	3.5 x 10 6	3	One	0	0	0	0	0	0	One
	Staphylococcus aureus	2.3x 10 6	2	One	0	0	0	0	0	0	One
	Pseudomonas aeruginosa	4.1 x 10 6	3	One	0	0	0	0	0	0	2
	Candida albicans	1.9 x 10 5	2	One	0	0	0	0	0	0	One
	Black fungus	3.8 x 10 5	2	2	0	0	0	0	0	0	One

0 = No Contamination (Sterile)

1 = Trace of contamination (1 ~ 9 Colonies per streak)

2 = Light contamination (10 ~ 99 Colonies per streak)

3 = Moderate contamination (Greater than 100 colonies but still distinguishable)

4 = Heavy contamination

As shown in Table 15, in the case of the industrial degreaser composition containing the antiseptic compositions of Examples 1 to 6 of the present invention, all bacteria and fungi were excellent in killing rate of 99.9% or more on the third day, but Comparative Example 1 In the fabric softener composition of 2 to 2, the antimicrobial activity against bacteria and fungi was insufficient, and thus did not die completely even on the 7th day. Therefore, it was confirmed that even when the industrial oil degreaser was used by mixing the SDS and CPC of the present invention in a specific ratio, it was possible to prepare an antiseptic composition having better antibacterial and antifungal effects than when only one component was used.

In particular, in Examples 5 to 6, both bacteria and fungi were killed on Day 2.

< Experimental Example  8> Confirmation of stability of antiseptic composition of the present invention

As in Examples 7 to 10 of Table 2 and Examples 11 to 22 of Table 3, each preservative containing SDS, CPC, Sodium sulfite, Hexylene Glycol and various surfactants The effect on the freezing point of the composition and the drop in recrystallization temperature was confirmed.

The temperature (recrystallization temperature) of the fine crystal cloud of the cloud-shaped cloud phenomenon was measured by dropping the temperature with the cooling water with respect to the 100 ml solution. These results are shown in Table 16 below.

Table 16

	Freezing Point O C	Recrystallization temperature O C		Freezing Point O C	Recrystallization temperature O C
Example 7	2.0	10.0	Example 15	-22.0	0.1
Example 8	-3.0	7.0	Example 16	-21.0	0.3
Example 9	-11.0	6.0	Example 17	-22.0	0.3
Example 10	-14.0	4.0	Example 18	-23.0	0.1
Example 11	-15.0	2.0	Example 19	-22.0	0.3
Example 12	-16.0	1.5	Example 20	-22.0	0.2
Example 13	-16.5	1.0	Example 21	-21.0	0.5
Example 14	-20.0	0.5	Example 22	-21.0	0.5

As shown in Table 16, rather than mixing only SDS and CPC to prepare an antiseptic composition, by mixing any one selected from the group consisting of sodium sulfite, hexylene glycol, surfactants and combinations thereof to prepare an antiseptic composition In this case, it was confirmed that the recrystallization temperature and the freezing point were lowered to prepare an antiseptic composition having excellent stability.

< Experimental Example  9> Confirm the skin hypoallergenic effect of the antiseptic composition of the present invention

In order to investigate the irritation to the skin of the antiseptic composition of the present invention was tested as follows. Prepare a solution containing purified water in Examples 1 to 22, Finn chamber (Finn chamber); 10 microliters were absorbed by NORGESPLASTER A / S and NORWAY paper disc, and it adhered to the lower part of arm of 20 subjects for 48 hours. After 2 hours after removing the Fin chamber (washing with running water), the redness of the skin was examined, the results are shown in Table 17.

Table 17

division	Total number of subjects / 20 people
	++	+	±	-
Example 1	-	-	-	20
Example 2	-	-	-	20
Example 3	-	-	-	20
Example 4	-	-	-	20
Example 5	-	-	-	20
Example 6	-	-	-	20
Example 7	-	-	-	20
Example 8	-	-	-	20
Example 9	-	-	-	20
Example 10	-	-	-	20
Example 11	-	-	-	20
Example 12	-	-	-	20
Example 13	-	-	-	20
Example 14	-	-	-	20
Example 15	-	-	-	20
Example 16	-	-	-	20
Example 17	-	-	-	20
Example 18	-	-	-	20
Example 19	-	-	-	20
Example 20	-	-	-	20
Example 21	-	-	-	20
Example 22	-	-	-	20

As shown in Table 17, all of the antiseptic compositions of Examples 1 to 22 were found to be not skin irritant.

As mentioned above, although an embodiment of the present invention has been described, those of ordinary skill in the art may add, change, delete or add components within the scope not departing from the spirit of the present invention described in the claims. The present invention may be modified and changed in various ways, etc., which will also be included within the scope of the present invention.

The antiseptic composition of the present invention may be a formulation selected from an aqueous solution, an emulsion, a disperse, a suspension and a useful solution, the antiseptic composition is a wet wipes composition, cosmetic composition, shampoo composition, detergent composition, fabric softener composition, industrial It can be used in detergent compositions and the like.

Claims (20)

1. Antiseptic composition comprising Sodium Metabisulfite (SDS) and Cetylpyridinium chloride (CPC) in a weight ratio of 1: 0.2 to 5.
2. The method of claim 1,

   The antiseptic composition is sodium sulfite (Sodium Sulfite), hexylene glycol (Hexylene Glycol), and further comprising any one selected from the group consisting of surfactants, combinations thereof, antiseptic composition.
3. The method of claim 2,

   The preservative composition is SDS and sodium sulfite in the case of sodium sulfite 1: 0.1 to 1.5 by weight, SDS and hexylene glycol in the weight ratio of 1: 0.1 to 1.5 for hexylene glycol, SDS and 1 to 0.1 to 300 of the surfactant, the antiseptic composition.
4. The method of claim 2,

   The surfactant may be an anionic surfactant of carboxylate, sulfate ester salt, sulfonate salt or phosphate ester salt; Polyethylene glycol-based or polyhydric alcohol-based non-ionic surfactants; And amino acid-based or betaine-based amphoteric surfactants.
5. The method according to any one of claims 1 to 4,

   The antiseptic composition is an antiseptic composition, wherein the formulation is selected from aqueous solutions, emulsions, dispersions, suspensions and useful solutions.
6. A wet wipe composition comprising the antiseptic composition according to any one of claims 1 to 4.
7. The method of claim 6,

   The antiseptic composition comprising 0.01% by weight to 10% by weight, the composition for wet wipes.
8. The method of claim 6,

   The composition for wet wipes further comprises any one selected from the group consisting of a moisturizer, a pH adjuster, a fragrance and a combination thereof, the composition for wet wipes.
9. Cosmetic composition comprising the antiseptic composition according to any one of claims 1 to 4.
10. The method of claim 9,

    Cosmetic composition comprising 0.1 to 10% by weight of the antiseptic composition.
11. The method of claim 9,

    The cosmetic composition further comprises any one selected from the group consisting of pH adjusters, moisturizers, fragrances, surfactants, additives, purified water and combinations thereof.
12. A shampoo composition comprising the antiseptic composition according to any one of claims 1 to 4.
13. The method of claim 12,

    Shampoo composition comprising 0.1 to 10% by weight of the antiseptic composition.
14. The method of claim 12,

    The shampoo composition further comprises any one selected from the group consisting of pH adjusters, moisturizers, fragrances, surfactants, additives, purified water and combinations thereof.
15. Detergent composition comprising an antiseptic composition according to any one of claims 1 to 4.
16. The method of claim 15,

    Containing the antiseptic composition 0.1 wt% to 10 wt%, detergent composition.
17. A composition for fabric softener comprising the antiseptic composition according to any one of claims 1 to 4.
18. The method of claim 17,

    The antiseptic composition comprises 0.1 wt% to 10 wt%, the composition for fabric softener.
19. An industrial detergent composition comprising the antiseptic composition according to any one of claims 1 to 4.
20. The method of claim 19,

    Industrial preservative composition comprising from 0.1% to 10% by weight of the antiseptic composition.