WO2016089062A2

WO2016089062A2 - Heterocyclic derivatives and use thereof

Info

Publication number: WO2016089062A2
Application number: PCT/KR2015/012926
Authority: WO
Inventors: Chan Hee Park; Sang Hwi Lee; Junhwan IM; Soon Ok Lee; Jungsook Kim; Heon Kyu Park; Jee Hun YUN; Kwang Seok Ko; Hye Jung Kim; Byungho Kim; Mi Sun Kim; Minjung KONG; Hyung Jo MOON
Original assignee: C&C Research Laboratories
Priority date: 2014-12-02
Filing date: 2015-11-30
Publication date: 2016-06-09
Also published as: WO2016089062A3

Abstract

A heterocyclic derivative represented by formula (I), or a pharmaceutically acceptable salt or a stereoisomer thereof, which has an inhibitory effect on the activation of STAT3 protein, and is useful for the prevention or treatment of diseases associated with the activation of STAT3 protein.

Description

DESCRIPTION

HETEROCYCLIC DERIVATIVES AND USE THEREOF

FIELD OF THE INVENTION

The present invention relates to novel heterocyclic compounds, uses thereof for the prevention or treatment of diseases associated with the activation of STAT proteins, particularly, STAT3 protein and pharmaceutical compositions comprising same.

BACKGROUND OF THE INVENTION Signal transducer and activator of transcription (STAT) proteins are transcription factors which transduce signals from various extracellular cytokines and growth factors to a nucleus. Seven (7) subtypes of STAT proteins (i.e., STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b and STAT6) are currently known, and generally they consist of about 750 - 850 amino acids. In addition, each subtype of STAT proteins contains several conserved domains which play an important role in exhibiting the function of STAT proteins. Specifically, five (5) domains from N-terminus to C-terminus of STAT proteins have been reported including coiled-coiled domain, DNA binding domain, linker domain, SH2 domain and transactivation domain (TAD)). Further, X-ray crystalline structures of STAT1, STAT3, STAT4 and STAT5 have been reported since 1998 (Becker S et al, Nature, 1998, 394; Vinkemeier U et al, Science, 1998, 279; Chen X et al, Cell, 1998, 93; D. Neculai et al., J. Biol. Chem., 2005, 280). In general, receptors to which cytokines and growth factors bind are categorized into Class I and Class II. IL-2, IL-3, IL-5, IL-6, IL- 12, G-CSF, GM-CSF, LIF, thrombopoietin, etc., bind to Class I receptors, while INF-a, INF-γ, IL-10, etc., bind to Class II receptors (Schindler C et al, Annu. Rev. Biochem., 1995, 64; Novick D et al, Cell, 1994, 77; Ho AS et al, Proc. Natl. Acad. Sci., 1993, 90). Among them, the cytokine receptors involved in the activation of STAT proteins can be classified depending on their structural forms of extracellular domains into a gp-130 family, an IL-2 family, a growth factor family, an interferon family and a receptor tyrosine kinase family. Interleukin-6 family cytokines are representative multifunctional cytokines which mediate various physiological activities. When interleukin-6 cytokine binds to IL-6 receptor which is present on the cell membrane surface, it attracts gp-130 receptor to form an IL-6-gp-130 receptor complex. At the same time, JAK kinases (JAKl, JAK2, JAK3 and Tyk2) in the cytoplasm are recruited to a cytoplasmic region of gpl30 to be phosphorylated and activated. Subsequently, latent cytoplasmic STAT proteins are attracted to a receptor, phosphorylated by JAK kinases and activated. Tyrosine-705 adjacent to the SH2 domain located in the C-terminus of STAT proteins is phosphorylated, and the activated tyrosine-705 of each STAT protein monomer binds to the SH2 domain of another monomer in a reciprocal manner, thereby forming a homo- or heterodimer. The dimers are translocalized into a nucleus and bind to a specific DNA binding promoter to promote the transcription. Through its transcription process, various proteins (Myc, Cyclin D1/D2, Bcl-xL, Mcl, survivin, VEGF, HIF-1, immune suppressors, etc.) associated with cell proliferation, survival, angiogenesis and immune evasion are produced (Stark et al., Annu. Rev. Biochem., 1997, 67; Levy et al., Nat. Rev. Mol. Cell Biol., 2002, 3).

In particular, STAT3 protein is known to play a crucial role in the acute inflammatory response and the signal transduction pathway of IL-6 and EGF (Akira et al. , Cell, 1994, 76; Zhong et al., Science, 1994, 264). According to the recent clinical report, STAT3 protein is constantly activated in patients with solid cancers occurring in prostate, stomach, breast, lung, pancreas, kidney, uterine, ovary, head and neck, etc., and also in patients with blood cancer such as acute and chronic leukemia, multiple myeloma, etc. Further, it has been reported that the survival rate of a patient group with activated STAT3 is remarkably lower than that of a patient group with inactivated STAT3 (Masuda et al., Cancer Res., 2002, 62; Benekli et al, Blood, 2002, 99; Yuichi et al, Int. J. Oncology, 2007, 30). Meanwhile, STAT3 was identified to be an essential factor for the growth and maintenance of murine embryonic stem cells in a study employing a STAT3 knockout mouse model. Also, a study with a tissue-specific ST AT3 -deficient mouse model reveals that STAT3 plays an important role in cell growth, apoptosis, and cell motility in a tissue- specific manner (Akira et al, Oncogene 2000, 19). Moreover, since apoptosis induced by anti-sensing STAT3 was observed in various cancer cell lines, STAT3 is considered as a promising new anticancer target. STAT3 is also considered as a potential target in the treatment of patients with diabetes, immune-related diseases, hepatitis C, macular degeneration, human papillomavirus infection, non-Hodgkin's lymphoma, tuberculosis, etc. Meanwhile, newly identified Thl7 cells have been reported through a number of recent articles to be associated with various autoimmune diseases (Jacek Tabarkiewicz et al., Arch. Immunol. Ther. Exp., 2015, 11). Based on these reports, a control of the differentiation and function of Thl7 cells is considered as a good target in the treatment of related diseases. In particular, since STAT3 -dependent IL-6 and IL-23 signal transductions are known as important factors in the differentiation of Thl7 cells (Xuexian O. Yang et al., J. Biol. Chem., 2007, 282; Harris T J et al., J. Immunol., 2007, 179), an inhibition of the function of STAT3 is expected to be effective in the treatment of diseases associated with Thl7 cells such as systemic lupus erythematosus, uveitis, rheumatoid arthritis, autoimmune thyroid disease, inflammatory bowel disease, psoriasis and psoriatic arthritis (Jacek Tabarkiewicz et al., Arch. Immunol. Ther. Exp., 2015, 11).

Recently, IL-6 and IL-23 antibodies are under clinical studies on the treatment of arthritis and psoriasis associated with Thl7 cells and exhibit a clinical efficacy (Nishimoto N. et al., Arthritis Rheum., 2004, 50; Gerald G. et al., N. Engl. J. Med., 2007, 356). This also confirms that the inhibition of STAT3 signal transduction is an effective therapeutic method for such diseases.

In contrast, while having intracellular response pathways of identical cytokines and growth factors to those of STAT3, STAT1 increases inflammation and congenital and acquired immunities to inhibit the proliferation of cancer cells or cause pro-apoptotic responses, unlike STAT3 (Valeria Poli et al, Review, Landes Bioscience, 2009).

In order to develop STAT3 inhibitors, the following methods can be considered: i) inhibition of the phosphorylation of STAT3 protein by IL-6/gp-130/JAK kinase, ii) inhibition of the dimerization of activated STAT3 proteins, and iii) inhibition of the binding of STAT3 dimer to nuclear DNA. Small molecular STAT3 inhibitors are currently under development. Specifically, OPB-31121 and OPB-51602 are under clinical studies on patients with solid cancers or blood cancers by Otsuka Pharmaceutical Co., Ltd. Further, S3I-201 (Siddiquee et al, Proc. Natl. Acad. Sci., 2007, 104), SSI- M2001 (Siddiquee et al, Chem. Biol, 2007, 2), LLL-12 (Lin et al, Neoplasia, 2010, 12), Stattic (Schust et al, Chem. Biol. 2006, 13), STA-21 (Song et al, Proc. Natl. Acad. Sci., 2005, 102), SF-1-066 (Zhang et al, Biochem. Pharm., 2010, 79) and STX-0119 (Matsuno et al, ACS Med. Chem. Lett., 2010, 1), etc. have been reported to be effective in a cancer cell growth inhibition experiment and in animal model (in vivo Xenograft model). Furthermore, although peptide compounds mimicking the sequence of amino acid of pY- 705 (STAT3) adjacent to the binding site to SH2 domain or the amino acid sequence of gp- 130 receptor in which JAK kinases bind were studied (Coleman et al, J. Med. Chem., 2005, 48), the development of the peptide compounds has not been successful due to the problems such as solubility and membrane permeability.

SUMMARY OF THE INVENTION

Accordingly, it is an object of the present invention to provide novel heterocyclic derivatives for the inhibition of the activation of STAT3 protein.

It is another object of the present invention to provide uses of the heterocyclic derivatives for the prevention or treatment of diseases associated with the activation of STAT3 protein.

In accordance with one aspect of the present invention, there is provided a compound selected from the group consisting of a heterocyclic derivative represented by formula (I), and a pharmaceutically acceptable salt and a stereoisomer thereof:

wherein

Xi and X₂ are each independently -C(-Rx)(-Rx")-, -C(-Rx')(-Rx")-, -C(-Rx")(- Rx")-, -C(=0)-, -N(Rx)-, -N(-Rx')-, -N(-Rx")-, or -0-;

O

Rs— S— I

II s

Rx is Xs ;

Xs is =O or =NH;

Rs is C_1-6alkyl, haloC_1-6alkyl, C_1-6alkoxy-C_1-6alkyl, C_1-6alkylcarbonyl-C_1-6alkyl, C_2-7alkenyl, amino, or aminoCi_-6alkyl;

Rx' is haloC_1- alkyl, C_1-4alkoxycarbonyl, cyano, nitro, azido, amino, or a 3- to 6- membered heterocyclyl unsubstituted or substituted with Rx";

Rx" is each independently hydrogen, halogen, nitro, amino, Cj_-6alkyl, C_1- alkoxy, haloCi_-6alkoxy, carbamoylCi_-6alkyl, Ci_-6alkylamino-Ci.₆alkyl, or diCi-ealkylamino-C_!. ₆alkyl;

one of Y and Z is -S- or -NH-, and the other is -CH= or -N=;

Lx is a saturated or unsaturated C_1-4 hydrocarbon chain not containing or containing 1 to 3 heterogroups selected from the group consisting of -0-, -NH-, -N=, -S-, - S(=0)- and -S(=0)₂- in the chain, and unsubstituted or substituted with at least one Rx" moiety;

A and B are each independently a monocyclic- or bicyclic-saturated or unsaturated C_3-iocarbocycle or 5- to 12-membered heterocycle;

Rc is =0, =NH, =N(-C,_-6alkyl), or =N(-OH);

RN is hydrogen or C_1-6alkyl;

L_B is -[C(-R_L)(-R_L')]_m-, -[C(-R_L)(-R_L')]_n-0-, -0-, -NH-, -N(C_1-6alkyl)-, -S(=0)₂-, - C(=0)-, or -C(=CH₂)-, wherein m is an integer of 0 to 3, n is an integer of 1 to 3, R_L and R_L' are each independently hydrogen, hydroxy, halogen or C_1-6alkyl, or R_L and R_L' are linked together to form Ci_-6alkylene;

R_A is hydrogen, halogen, cyano, C_1-6alkyl, haloC_1-6alkyl, cyanoC_1-6alkyl, Q. ₆alkylcarbonyl, C_1-6alkoxy, haloC_1-6alkoxy, cyanoC_1-6alkoxy, C_1-6alkylamino, diCi. ₆alkylamino, C_1-6alkylthio, C_1-6alkylaminocarbonyl, diC_1-6alkylaminocarbonyl, C_2- ₈alkynyl, Ci_-6alkoxycarbonylamino-Ci_-6alkoxy, aminoC_1-6alkoxy, or 3- to 6-membered heterocyclyl;

R_B is hydrogen, halogen, hydroxy, cyano, nitro, amino, oxo, aminosulfonyl, sulfonylamido, d^alkylamino, C_]-6alkyl, haloCi_-6alkyl, cyanoC_1- alkyl, Ci_-6alkoxy, haloCi_-6alkoxy, cyanoCi_-6alkoxy, C_3-8cycloalkyloxy, C_2-8alkenyl, C_2-8alkenyloxy, C_2- salkynyl, C_2-8alkynyloxy, Ci_-6alkylamino-Ci_-6alkoxy, diCi_-6alkylamino-C_1-6alkoxy, Ci. ₆alkoxy-carbonyl, carbamoyl, carbamoyl-C_1-6alkoxy, C_1- alkylthio, C_1-6alkylsulfinyl, Q. ₆alkylsulfonyl, 5- to 10-membered heterocyclyl, 5- to 10-membered heterocyclyl -Ci_-6alkyl, 5- to 10-membered heterocyclyl-C i_-6alkoxy, or 5- to 10-membered heterocyclyl-oxy;

p is an integer of 0 to 4, and, when p is 2 or higher, RA moieties are the same as or different from each other;

q is an integer of 0 to 4, and, when q is 2 or higher, RB moieties are the same as or different from each other; and

each of said heterocycle and heterocyclyl moieties independently contains at least one heterogroup selected from the group consisting of -0-, -NH-, -N=, -S-, -S(=0)- and - S(=0)₂-.

In accordance with another aspect of the present invention, there is provided a use of a compound selected from the group consisting of a heterocyclic derivative represented by formula (I) above, and a pharmaceutically acceptable salt and a stereoisomer thereof for the manufacture of a medicament for preventing or treating diseases associated with the activation of STAT3 protein.

In accordance with a further aspect of the present invention, there is provided a pharmaceutical composition for preventing or treating diseases associated with the activation of STAT3 protein, comprising a compound selected from the group consisting of a heterocyclic derivative represented by formula (I) above, and a pharmaceutically acceptable salt and a stereoisomer thereof as active ingredients.

In accordance with a still further aspect of the present invention, there is provided a method for preventing or treating diseases associated with the activation of STAT3 protein in a mammal, which comprises administering a compound selected from the group consisting of a heterocyclic derivative represented by formula (I) above, and a pharmaceutically acceptable salt and a stereoisomer thereof to the mammal.

The heterocyclic derivative represented by formula (I) above, or a pharmaceutically acceptable salt or a stereoisomer thereof has an excellent inhibitory effect on the activation of STAT3 protein, and thus it can be used for the prevention or treatment of diseases associated with the activation of STAT3 protein.

DETAILED DESCRIPTION OF THE INVENTION

The present invention will be further described in detail herein below.

In the specification of the present invention, the term "halogen" refers to fluoro, chloro, bromo or iodo, unless specified otherwise.

The term "alkyl" refers to a linear or branched hydrocarbon moiety, unless specified otherwise.

The terms "haloalkyl", "haloalkoxy", "halophenyl", etc., respectively refer to alkyl, alkoxy, and phenyl substituted with at least one halogen.

The term "carbocycle" refers to an aromatic or non-aromatic hydrocarbon ring, which may be saturated or unsaturated, and a monocyclic or polycyclic radical. The term "carbocyclyl" refers to a radical of "carbocycle", and is used as a term inclusive of "cycloalkyl" and "aryl". The term "cycloalkyl" refers to a saturated hydrocarbon radical, which may be monocyclic or polycyclic. The term "aryl" refers to an aromatic hydrocarbon ring, which may be monocyclic or polycyclic.

The terms "carbocycle", "carbocyclyl", "cycloalkyl" and "aryl" may refer to, for example, a monocycle or polycycle having 3 to 20 carbon atoms, and will be indicated as "C_3-20 carbocycle", "C_3-20 carbocyclyl", "C_3-20 cycloalkyl", and "C_3-20 aryl", respectively.

The term "heterocycle" refers to an aromatic or non-aromatic ring having at least one heteroatom, which may be saturated or unsaturated, and a monocycle or polycycle. The term "heterocyclyl" refers to a radical of "heterocycle", which is used as a term inclusive of "heterocycloalkyl" and "heteroaryl". The term "heterocycloalkyl" refers to a saturated ring radical having at least one heteroatom, which may be monocyclic or polycyclic. The term "heteroaryl" refers to an aromatic ring radical having at least one heteroatom, which may be monocyclic or polycyclic.

The term "heteroatom" may be selected from N, O and S.

The terms "heterocycle", "heterocyclyl", "heterocycloalkyl" and "heteroaryl" may refer to, for example, a mono- or polycycle having 3 to 20 heteroatoms and/or carbon atoms, and will be indicated as "3- to 20-membered heterocycle", "3- to 20-membered heterocyclyl", "3- to 20-membered heterocycloalkyl", and "3- to 20-membered heteroaryl".

The terms "chain" refers to a saturated or unsaturated C_2-10 hydrocarbon chain not containing any heteroatoms in the chain, for example, ethylene, propylene, butylene and - CH₂-CH=CH-; or a saturated or unsaturated C_2-i₀ hydro carbon chain containing at least one heterogroup selected from the group consisting of -0-, -NH-, -N=, -S-, -S(=0)- and - S(=0)₂- in the chain, for example, -CH₂-0-CH₂-, -CH₂-0-CH₂-0-CH₂-, -CH₂-CH=CH- NH- and -CH₂-CH₂-S(=0)₂-CH₂-0-, unless specified otherwise. The chain may be substituted with at least one selected from the group consisting of halogen, C_1-6alkyl and Cj_-6alkoxy.

wherein

X, and X₂ are each independently -C(-Rx)(-Rx")-, -C(-Rx')(-Rx")-, -C(-Rx")(- Rx")-, -C(=0)-, -N(Rx)-, -N(-Rx')-, -N(-Rx")-, or -0-; Rs— S— I

II s

Rx is Xs .

Xs is =O or =NH;

Rs is Ci- alkyl, haloCi^alkyl, C_1-6alkoxy-Ci-₆alkyl, Ci_-6alkylcarbonyl-Ci_-6alkyl, C_2-7alkenyl, amino, or aminoC_1-6alkyl;

Rx' is haloCi_-6alkyl,

cyano, nitro, azido, amino, or a 3- to 6- membered heterocyclyl unsubstituted or substituted with Rx";

Rx" is each independently hydrogen, halogen, nitro, amino, Ci_-6alkyl, C_1-6alkoxy, haloC_1-6alkoxy, carbamoylC]- alkyl, C_1-6alkylamino-C_1- alkyl, or diCi-ealkylamino-Ci. ₆alkyl;

one of Y and Z is -S- or -NH-, and the other is -CH= or -N=;

A and B are each independently a monocyclic- or bicyclic-saturated or unsaturated

C3-i₀carbocycle or 5- to 12-membered heterocycle;

Rc is =0, =NH, =N(-Ci_-6alkyl), or =N(-OH);

R is hydrogen or Ci_-6alkyl;

L_B is -[C(-R_L)(-R_L')]_m-, -[C(-R_L)(-R_L')]_n-0-, -0-, -NH-, -N(C_1-6alkyl)-, -S(=0)₂-, - C(=0)-, or -C(=CH₂)-, wherein m is an integer of 0 to 3, n is an integer of 1 to 3, R_L and RL' are each independently hydrogen, hydroxy, halogen or C_1- alkyl, or R_L and RL' are linked together to form Ci_-6alkylene;

R_A is hydrogen, halogen, cyano, C_1-6alkyl, haloCi_-6alkyl, cyanoCi-₆alkyl, Ci. ₆alkylcarbonyl, C_1- alkoxy, haloC_1-6alkoxy, cyanoCi_-6alkoxy, Ci-ealkylamino, diCj. ₆alkylamino, C_1-6alkylthio, Ci_-6alkylaminocarbonyl, diC_1-6alkylaminocarbonyl, C_2- ₈alkynyl, C^alkoxycarbonylamino-Q-ealkoxy, aminoC_1-6alkoxy, or 3- to 6-membered heterocyclyl;

R_B is hydrogen, halogen, hydroxy, cyano, nitro, amino, oxo, aminosulfonyl, sulfonylamido, C_1-6alkylamino, C_1-6alkyl, haloC_1-6alkyl, cyanoC_1-6alkyl, C_1-6alkoxy, haloC_1-6alkoxy, cyanoC_1-6alkoxy, C_3-8cycloalkyloxy, C₂-₈alkenyl, C_2-8alkenyloxy, C_2- ₈alkynyl, C_2-galkynyloxy, Ci_-6alkylamino-C_1- alkoxy, diCi_-6alkylamino-C_1-6alkoxy, Ci_ ₆alkoxy-carbonyl, carbamoyl, carbamoyl-Ci_-6alkoxy, Ci_-6alkylthio,

Ci. ₆alkylsulfonyl, 5- to 10-membered heterocyclyl, 5- to 10-membered heterocyclyl-Ci_-6alkyl, 5- to 10-membered heterocyclyl-C_1- alkoxy, or 5- to 10-membered heterocyclyl-oxy;

q is an integer of 0 to 4, and, when q is 2 or higher, RB moieties are the same as or different from each other; and each of said heterocycle and heterocyclyl moieties independently contains at least one heterogroup selected from the group consisting of -0-, -NH-, -N=, -S-, -S(=0)- and - S(=0)₂-.

In a preferred embodiment of the compound of formula (I),

one of Y and Z is -S- or -NH-, and the other is -CH=;

Lx is a saturated C_1-3 hydrocarbon chain not containing or containing at least one heteroatom selected from the group consisting of O, N and S in the chain, and unsubstituted or substituted with at least one substituent selected from the group consisting of halogen, C_1-6alkyl and C_1-6alkoxy;

one of Xi and X₂ is -C(-Rx)(-Rx")-, -C(-Rx')(-Rx")-, -C(=0)-, -N(Rx)- or -N(- Rx')-, and the other is -C(-Rx")(-Rx")-, -N(-Rx")- or -0-;

O

Rs-S—

II s I

Rx is ^Xs ;

Xs is =0 or =NH;

Rs is C_1-6alkyl or haloC_1-6alkyl;

Rx' is haloC_1-6alkyl, cyano, nitro, amino, azido, or a 5- to 6-membered heterocyclyl containing at least one heteroatom selected from the group consisting of N, S and O and unsubstituted or substituted with oxo;

Rx" is hydrogen, halogen, Ci_-6alkyl, or C_1-4alkoxycarbonyl; and

Rc, RN, A, B, L_B, RA, RB, p and q are the same as defined above in formula (I).

In a preferred embodiment of the compound of formula (I),

Y is -CH=;

Z is -S-;

Rc is =0;

RN is hydrogen;

Lx is a saturated Ci_-3 hydrocarbon chain not containing or containing oxygen atom in the chain, and unsubstituted or substituted with at least one substituent selected from the group consisting of halogen, d-ealkyl and C_1-6alkoxy;

Xi is -C(-Rx)(-Rx")-, -C(-Rx')(-Rx")-, or -N(Rx)-;

X₂ is -C(-Rx")(-Rx")-, -C(=0)-, -N(-Rx")-, or -0-;

O

II I

H₃C-S— I

3 II ¾

Rx is ^Xs ;

Xs is =0 or =NH;

Rx' is haloCi_-6alkyl, cyano, nitro, amino, azido, or a 5- to 6-membered heterocyclyl containing 1 to 2 heteroatoms selected from N and O and unsubstituted or substituted with oxo; Rx" is hydrogen, halogen, C_1- alkyl, or Ci_-4alkoxycarbonyl; and

A, B, LB, RA, RB, P and q are the same as defined above in formula (I).

In a preferred embodiment of the compound of formula (I),

Y is -CH=;

Z is -S-;

Rc is =0;

RN is hydrogen;

Lx is a saturated C_1-3 hydrocarbon chain not containing or containing oxygen atom in the chain, and unsubstituted or substituted with at least one substituent selected from the group consisting of halogen, C^alkyl and Ci_-6alkoxy;

X, is -C(-Rx)(-Rx")-, -C(-Rx')(-Rx")-, or -N(Rx)-;

X₂ is -C(-Rx")(-Rx"h -C(=0)-, -N(-Rx")-, or -0-;

O

H₃c-s— I

Rx is Xs _;

Xs is =0 or =NH;

Rx' is haloC_1-6alkyl, cyano, nitro, amino, azido, or a 5- to 6-membered heterocyclyl containing 1 to 2 heteroatoms selected from N and O and unsubstituted or substituted with oxo;

Rx" is hydrogen, halogen, C_1-6alkyl, or C_1-4alkoxycarbonyl;

A is benzene or a 5- to 10-membered heteroaryl containing 1 to 3 nitrogen atoms;

B is a monocyclic- or bicyclic-saturated or unsaturated C_6-10carbocycle or 5- to 10- membered heterocycle;

L_B is -[C(-R_L)(-R_L')]m-, -0-, -NH- or -N(C_1-6alkyl)-, wherein m is 0 or 1, R_L and R_L' are each independently hydrogen, hydroxy, halogen or C_1-6alkyl, or R_L and R_L' are linked together to form C_2-5alkylene;

R_A is halogen, C_1-6alkoxycarbonylamino-C_1-6alkoxy, aminoC_1-6alkoxy, or 3- to 6- membered heterocyclyl;

RB is halogen, C_1-6alkyl, C_1-6alkoxy, haloC_1-6alkyloxy, C2_-6alkenyloxy, C_3- _!ocarbocyclyl-oxy, or 3- to 10-membered heterocyclyl-C_1-3alkoxy; and

each of said heteroaryl, heterocycle and heterocyclyl moieties independently contains 1 to 3 heteroatoms selected from the group consisting of O, N and S.

In a preferred embodiment of the compound of formula (I),

X, is -N(-Rx)-;

X₂ is -C(-Rx'^*)(-Rx")- or -N(-Rx")-;

Y is -CH=;

Z is -S-;

Rc is =0; RN is hydrogen;

Lx is ethylene substituted with one or two Rx" moieties,

O

Rs— S— I

II «

Rx is Xs _;

Xs is =0;

Rs is methyl;

Rx" is the same as defined above in formula (I); and

A, B, L_B, RA, RB, P and q are the same as defined above in formula (I).

In a preferred embodiment of the compound of formula (I),

Xi is -CH(-Rx)-;

X₂ is -N(-Rx")-;

Y is -CH=;

Z is -S-;

Rc is =0;

R_N is hydrogen;

Lx is ethylene;

O

II I

Rs-S— f

II §

Rx is ^Xs ;

Xs is =0;

Rs is methyl;

Rx" is the same as defined above in formula (I); and

A, B, L_B, RA, R_B, p and q are the same as defined above in formula (I).

In a preferred embodiment of the compound of formula (I),

Xi is -C(-Rx)(-Rx")-;

X₂ is -0-;

Y is -CH=;

Z is -S-;

Rc is =0;

R_N is hydrogen;

Lx is ethylene;

O

II I

Rs-S— I

II ^¾

Rx is ^Xs ;

Xs is =0;

Rs is methyl;

Rx" is the same as defined above in formula (I); and A, B, L_B, RA, RB, p and q are the same as defined above in formula (I).

In a preferred embodiment of the compound of formula (I),

X, is -C(-Rx')(-Rx")-;

X₂ is -0-;

Y is -CH=;

Z is -S-;

Rc is =0;

R is hydrogen;

Lx is ethylene;

Rx' and Rx" are the same defined above in formula (I); and

A, B, L_B, R_A, RB, p and q are the same as defined above in formula (I).

In a preferred embodiment of the compound of formula (I),

X₂ is -C(-Rx")(-Rx")- or -C(=0)-;

Y is -CH=;

Z is -S-;

Rc is =0;

RN is hydrogen;

Lx is ethylene;

O

Rs—S— I

II §

Rx is ^Xs ;

Xs is =0;

Rs is methyl;

Rx" is the same as defined above in formula (I); and

A, B, L_B, R_A, R_B, p and q are the same as defined above in formula (I).

In a preferred embodiment of the compound of formula (I),

X_! is -CH(-Rx)-;

X₂ is -C(-Rx")(-Rx")-;

Y is -CH=;

Z is -S-;

Rc is =0;

RN is hydrogen;

Lx is -CH₂-0-;

O

Rs-S— I

II *

Rx is ^Xs ; Xs is =0;

Rs is methyl;

Rx" is the same as defined above in formula (I); and

A, B, LB, RA, RB, P and q are the same as defined above in formula (I).

In a preferred embodiment of the compound of formula (I),

Xi is -C(-Rx)(-Rx")- or -N(Rx)-;

X₂ is -0-;

Y is -NH-;

Z is -CH=;

Rc is =0;

RN is hydrogen;

Lx is propylene;

Rx and Rx" are the same as defined above in formula (I); and

A, B, LB, RA, RB, p and q are the same as defined above in formula (I).

Preferable examples of the compound according to the present invention are listed below, and a pharmaceutically acceptable salt and a stereoisomer thereof are also included in the scope of the present invention:

1) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-l-(methylsulfonyl)-2,3-dihydro- l /-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxamide;

2) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-3,3-dimethyl-l- (methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxamide;

3) V-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)- 1 -(methylsulfonyl)- 1 ,2,3 ,4- tetrahydrothieno[3',2':4,5]benzo[ 1 ,2-b][ 1 ,4]oxazepine-8-carboxamide;

4) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-8,8-dimethyl-5- (methylsulfonyl)-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2-carboxamide;

5) tert-butyl 7-((2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)carbamoyl)-l - (methylsulfonyl)-2,3-dihydrothieno[2,3-g]quinoxaline-4(lH)-carboxylate;

6) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-5-(methylsulfonyl)-5, 6,7,8- tetrahydrothieno [2,3 -g] quinoline-2-carboxamide;

7) 7Y-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-methyl- 1 -(methylsulfonyl)- 1 ,2,3 ,4-tetrahydrothieno [2,3 -g] quinoxaline-7-carboxamide;

8) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4-dihydro- 2H-thieno [3 ,2-g] chromene-7-carboxamide;

9) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-5-(methylsulfonyl)-2,3,4,5- tetrahydrothieno[3',2':4,5]benzo[l,2-b]oxepine-8-carboxamide;

10) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-5-(methylsulfonyl)-5, 6,7,8- tetrahydronaphtho[2,3-b]thiophene-2-carboxamide;

11) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-methyl-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

12) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-5-(methylsulfonyl)-5,8- dihydro-6H-thieno[3,2-g]isochromene-2-carboxamide;

13) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-fluoro-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

14) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-8,8-difluoro-5- (methylsulfonyl)-5,6,7,8-tetrahydronaphtho[2,3-b]thiophene-2-carboxamide;

15) N-(2-chloro-6-(p-tolyloxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4-dihydro-2H- thieno[3,2-g]chromene-7-carboxamide;

16) N-(2-chloro-6-(3-(trifluoromethyl)phenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno[3 ,2-g] chromene-7-carboxamide;

17) N-(2-chloro-6-(4-(trifluoromethyl)phenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno[3 ,2-g] chromene-7-carboxamide;

18) N-(2-chloro-6-(3,5-dichlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

19) N-(2-chloro-6-(4-chloro-3-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

20) N-(2-chloro-6-(4-chloro-3-methylphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

21) N-(2-chloro-6-(4-chloro-2-methylphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

22) N-(2-chloro-6-(4-methoxyphenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

23) N-(2-chloro-6-(4-chloro-2-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

24) N-(2-chloro-6-(3,4-dichlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

25) N-(2-chloro-6-(3-chlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

26) N-(2-chloro-6-(4-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4-dihydro- 2H-thieno[3,2-g]chromene-7-carboxamide;

27) N-(2-chloro-6-(3-chloro-4-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

28) N-(2-chloro-6-(4-(trifluoromethoxy)phenoxy)pyridin-4-yl)-4- (methylsulfonyl)-3,4-dihydro-2//-thieno[3,2-g]chromene-7-carboxamide;

29) N-(2-chloro-6-(3-(trifluoromethoxy)phenoxy)pyridin-4-yl)-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

30) N-(2-chloro-6-(3-chloro-5-methoxyphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

31) N-(2-chloro-6-(3-chloro-5-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g-]chromene-7-carboxamide;

32) N-(2-chloro-6-(3-fluoro-5-methoxyphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

33) N-(2-chloro-6-(m-tolyloxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4-dihydro-2H- thieno[3,2-g]chromene-7-carboxamide;

34) N-(2-chloro-6-(3,4-difluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

35) N-(2-chloro-6-(5-chloro-2-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

36) N-(2-chloro-6-(3-chloro-2-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g-]chromene-7-carboxamide;

37) N-(2-chloro-6-(5-chloro-2-methylphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

38) N-(2-chloro-6-(3 -chloro-4-methylphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

39) N-(2-chloro-6-(2-(trifluoromethyl)phenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

40) N-(2-chloro-6-(2-(trifluoromethoxy)phenoxy)pyridin-4-yl)-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

41) N-(2-chloro-6-(2-fluoro-3-methylphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

42) N-(2-chloro-6-(4-chloro-2-methoxyphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

43) (5)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

44) (i?)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

45) (5)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-fluoro-4- (methylsulfonyl)-3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

46) (R)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-fluoro-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

47) (,S)-iV-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-methyl-4- (methylsulfonyl)-3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

48) (R)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-methyl-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

49) (5)-N-(2-chloro-6-(3-chloro-5-methoxyphenoxy)pyridin-4-yl)-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

50) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-l-(methylsulfonyl)-l,2,3,4- tetrahydrothieno[2,3-g]quinoxaline-7-carboxamide;

51) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-5-(methylsulfonyl)-8-oxo- 5,6,7,8-tetrahydronaphtho[2,3-b]thiophene-2-carboxamide;

52) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-( lH-pyrazol- 1 -yl)-3 ,4- dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

53) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-(2-oxopyrrolidin-l-yl)-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

54) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-cyano-3,4-dihydro-2H- thieno [3 ,2-g] chromene-7-carboxamide;

55) 4-azido-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-3,4-dihydro-2H- thieno[3,2-g]chromene-7-carboxamide; and

56) N-(3-chloro-5-(2-(4-chlorophenyl)propan-2-yl)phenyl)-l -(methylsulfonyl)-

2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxamide.

The above-listed names of the compounds are described in accordance with the nomenclature method provided by ChemBioDraw Ultra software (Version 13.0.0.3015) of PerkinElmer.

The present invention provides a pharmaceutically acceptable salt of a heterocyclic derivative represented by formula (I) above. The pharmaceutically acceptable salt should have low toxicity to humans, and should not have any negative impact on the biological activities and physicochemical properties of parent compounds. Examples of the pharmaceutically acceptable salt may include an acid addition salt between a pharmaceutically usable free acid and a basic compound represented by formula (I), an alkaline metal salt (sodium salt, etc.) and an alkaline earth metal salt (potassium salt, etc.), an organic base addition salt between an organic base and carboxylic acid represented by formula (I), amino acid addition salt, etc.

Examples of a suitable form of salts according to the present invention may be a salt with an inorganic acid or organic acid, wherein the inorganic acid may be hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, perchloric acid, bromic acid, etc., and the organic acid may be acetic acid, methanesulfonic acid, efhanesulfonic acid, p- toluenesulfonic acid, fumaric acid, maleic acid, malonic acid, phthalic acid, succinic acid, lactic acid, citric acid, gluconic acid, tartaric acid, salicylic acid, malic acid, oxalic acid, benzoic acid, embonic acid, aspartic acid, glutamic acid, etc. The organic base which may be used for the preparation of the organic base addition salt may include tris(hydroxymethyl)methylamine, dicyclohexylamine, etc. Amino acids which may be used for the preparation of amino acid addition base may include natural amino acids such as alanine, and glycine.

The salts may be prepared using a conventional method. For example, the salts may be prepared by dissolving the compound represented by formula (I) in a water- miscible solvent such as methanol, ethanol, acetone, and 1 ,4-dioxane, adding a free acid or a free base, and then crystallizing the resultant thereafter. Additionally, the compounds of the present invention may have a chiral carbon center, and thus they may be present in the form of an R or S isomer, a racemic compound, an individual enantiomer or a mixture, an individual diastereomer or a mixture, and all these stereoisomers and a mixture thereof may belong to the scope of the present invention.

Additionally, the compounds of the present invention may also include a hydrate or solvate of the heterocyclic derivative represented by formula (I). The hydrate or solvate may be prepared using a known method, and they are preferred to be non-toxic and water- soluble, and in particular, they are preferably water or a hydrate or solvate having 1-5 molecules of alcoholic solvent (especially ethanol, etc.) bound thereto.

The present invention also provides a use of a compound selected from the group consisting of a heterocyclic derivative represented by formula (I) above, and a pharmaceutically acceptable salt and a stereoisomer thereof for the manufacture of a medicament for preventing or treating diseases associated with the activation of STAT3 protein.

Further, the present invention provides method for preventing or treating diseases associated with the activation of STAT3 protein in a mammal, which comprises administering a compound selected from the group consisting of a heterocyclic derivative represented by formula (I) above, and a pharmaceutically acceptable salt and a stereoisomer thereof to the mammal.

Further, the present invention provides a pharmaceutical composition for preventing or treating diseases associated with the activation of STAT3 protein, comprising a compound selected from the group consisting of a heterocyclic derivative represented by formula (I) above, and a pharmaceutically acceptable salt and a stereoisomer thereof as active ingredients.

Specifically, the diseases associated with the activation of STAT3 protein is selected from the group consisting of solid cancers, hematological or blood cancers, radio- or chemo-resistant cancers, metastatic cancers, inflammatory diseases, immunological diseases, diabetes, macular degeneration, human papillomavirus infection and tuberculosis.

More specifically, the diseases associated with the activation of STAT3 protein are selected from the group consisting of breast cancer, lung cancer, stomach cancer, prostate cancer, uterine cancer, ovarian cancer, kidney cancer, pancreatic cancer, liver cancer, colon cancer, skin cancer, head and neck cancer, thyroid cancer, osteosarcoma, acute or chronic leukemia, multiple myeloma, B- or T-cell lymphoma, non-Hodgkin's lymphoma, autoimmune diseases comprising rheumatoid arthritis, psoriasis, hepatitis, inflammatory bowel disease, Crohn's disease, diabetes, macular degeneration, human papillomavirus infection, and tuberculosis. In particular, a heterocyclic derivative represented by formula (I) above, or a pharmaceutically acceptable salt or a stereoisomer thereof has an excellent inhibitory effect on the activation of STAT3 protein, and thus the present invention also provides a composition for the inhibition of STAT3 protein comprising the same as an active ingredient.

The pharmaceutical composition of the present invention, in addition to the heterocyclic derivative represented by formula (I) above, the pharmaceutically acceptable salt thereof, or the stereoisomer thereof, may further include as active ingredients, common and non-toxic pharmaceutically acceptable additives, for example, a carrier, an excipient, a diluent, an adjuvant, etc., to be formulated into a preparation according to a conventional method.

The pharmaceutical composition of the present invention may be formulated into various forms of preparations for oral administration such as tablets, pills, powders, capsules, syrups, or emulsions, or for parenteral administration such as intramuscular, intravenous or subcutaneous injections, etc., and preferably in the form of a preparation for oral administration.

Examples of the additives to be used in the pharmaceutical composition of the present invention may include sweeteners, binders, solvents, solubilization aids, wetting agents, emulsifiers, isotonic agents, absorbents, disintegrating agents, antioxidants, preservatives, lubricants, fillers, flavoring agents, etc. For example, they may include, lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine, silica, talc, stearic acid, stearin, magnesium stearate, magnesium alluminosilicate, starch, gelatin, gum tragacanth, alginic acid, sodium alginate, methylcellulose, sodium carboxymethylcellulose, agar, water, ethanol, polyethylene glycol, polyvinylpyrrolidone, sodium chloride, calcium chloride, orange essence, strawberry essence, vanilla flavor, etc.

The pharmaceutical composition of the present invention may be formulated into a preparation for oral administration by adding additives to active ingredients, wherein the additives may include cellulose, calcium silicate, corn starch, lactose, sucrose, dextrose, calcium phosphate, stearic acid, magnesium stearate, calcium stearate, gelatin, talc, surfactants, suspension agents, emulsifiers, diluents, etc.

The pharmaceutical composition of the present invention may be formulated into a preparation for injection by adding additives to the active ingredients, for example, water, a saline solution, a glucose solution, an aqueous glucose solution analog, alcohol, glycol, ether, oil, fatty acid, fatty acid ester, glyceride, surfactants, suspension agents, emulsifiers, etc.

The compound of the present invention may be administered preferably in an amount ranging from 0.1 to 2,000 mg/day based on an adult subject with 70 kg body weight. The compound of the present invention may be administered once daily or a few divided doses. The dosage of the compound of the present invention may vary depending on the health conditions, age, body weight, sex of the subject, administration route, severity of illness, etc., and the scope of the present invention will not be limited to the dose suggested above.

Example

Hereinafter, the present invention is described more specifically by the following examples, but these are provided only for illustration purposes and the present invention is not limited thereto.

The definition of the abbreviations used in the following examples is as follows.

[Table 1]

Abbreviation Full name

A1C1₃ Aluminum chloride

ACN Acetonitrile

AcOH Acetic acid

AD3N 2,2'-Azobis(2-methylpropionitrile)

BH₃-THF complex Borane tetrahydrofuran complex

BINAP 2,2'-Bis(diphenylphosphino)-l , 1 '-binaphthyl

BBr₃ Boron tribromide

Boc₂0 Di-/eri-butyl dicarbonate

Brine is water saturated or nearly saturated with a brine salt (generally,

Brine

sodium chloride)

CH₃CN Acetonitrile

CDCI3 Deuterated chloroform

CH₂C1₂ Dichloromethane

CH3I Methyl iodide

CH₃S0₂C1 Methanesulfonyl chloride

(COCl)₂ Oxalyl chloride

Cs₂C0₃ Cesium carbonate

Cu₂0 Copper (I) oxide

DAST (Diethylamino)sulfur trifluride

DIPEA N,N-diisopropylethylamine

DMA N,N-dimethylacetamide

DME 1 ,2-Dimethoxyethane

DMF N,N-dimethylformamide

DMSO Dimethylsulfoxide

DMSO-^e Dimethylsulfoxide-i/₆

EtOAc Ethyl acetate

EtOH Ethyl alcohol

Intermediate 1) Synthesis of l-(methylsulfonyl)-2,3-dihydro-lH- thieno[3',2':4,5]benzo[l,2-6] [l,4]oxazine-7-carboxylic acid

(a) Synthesis of 2-fluoro-4-methoxy-5-nitrobenzaldehyde

2-Fluoro-4-methoxybenzaldehyde (1.0 g, 6.49 mmol) was dissolved in cone. H₂S0₄ (6.0 mL), and 70% ΗΝ0₃ aqueous solution (0.8 mL, 6.49 mmol) and cone. H₂S0₄ (0.8 mL, 14.92 mmol) were slowly added at -15°C. The reaction mixture was stirred at - 15°C for 2 hours and poured into ice water. The precipitate was filtered and dissolved in CH₂C1₂ and neutralized with sat. NaHC0₃ aqueous solution. The organic extract was dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, rc-Hex : CH₂C1₂ = 3 : 1) to obtain 2- fluoro-4-methoxy-5-nitrobenzaldehyde (1.2 g, 91%) as a white solid.

Ή-NMR (400MHz, CDC1₃): δ 10.21 (s, 1H), 8.46 (d, 1H, J=7.2Hz), 6.88 (d, 1H, J=11.6Hz), 4.06 (s, 3H) (b) Synthesis of methyl 6-methoxy-5-nitrobenzo[b]thiophene-2-carboxylate

2-Fluoro-4-methoxy-5-nitrobenzaldehyde (1.2 g, 6.43 mmol) was dissolved in anhydrous DMF(16.0 mL), and methyl 2-mercaptoacetate (575.0 μί, 6.43 mmol) and 2C0₃ (1.8 g, 12.80 mmol) were added. The reaction mixture was stirred at 80°C for 2 hours, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure to obtain methyl 6-methoxy-5-nitrobenzo[b]thiophene-2-carboxylate (1.5 g) as a yellow solid without purification.

1H-NMR (400MHz, CDC1₃): δ 8.34 (s, 1H), 8.03 (s, 1H), 7.47 (s, 1H), 4.04 (s, 3H), 3.96 (s, 3H)

(c) Synthesis of methyl 5-amino-6-methoxybenzo[b]thiophene-2-carboxylate Methyl 6-methoxy-5-nitrobenzo[b]thiophene-2-carboxylate (1.3 g, 4.83 mmol) was dissolved in a mixture of MeOH/H₂0 (44.0 mL, 10/1 v/v), and Zn (3.1 g, 65.30 mmol) and NH₄C1 (2.6 g, 53.40 mmol) were added at room temperature. The reaction mixture was stirred at room temperature for 15 hours, filtered through Celite, and concentrated under reduced pressure. The residue was purified by flash column chromatography (amine silica gel, n-Hex : EtOAc = 4 : 1) to obtain methyl-5-amino-6- methoxybenzo[b]thiophene-2-carboxylate (1.1 g, 93%) as a yellow solid.

1H-NMR (400MHz, CDC1₃): δ 7.84 (s, 1H), 7.17 (s, 1H), 7.12 (s, 1H), 3.94-3.96 (m, 5H), 3.91 (s, 3H)

(d) Synthesis of methyl 5-amino-6-hydroxybenzo[b]thiophene-2-carboxylate Methyl 5-amino-6-methoxybenzo[b]thiophene-2-carboxylate (500.0 mg, 4.83 mmol) was dissolved in CH₂C1₂ (40.0 mL), and 1M solution of BBr₃ in CH₂C1₂ (6.7 mL, 6.74 mmol) was added at 0°C. The reaction mixture was stirred at room temperature for 30 minutes, H₂0 was added, and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1 % formic acid in CH3CN : 0.1% formic acid in H₂0) to obtain methyl 5-amino-6- hydroxybenzo[i»]thiophene-2-carboxylate (398.0 mg, 85%) as a gray solid.

LC/MS ESI (+): 224 (M+l)

Ή-NMR (400MHz, OMSO-d₆): δ 10.15 (brs, 1H), 7.85 (s, 1H), 7.15 (s, 1H), 7.07 (s, 1H), 4.86 (brs, 2H) 3.82 (s, 3H) (e) Synthesis of methyl 2,3-dihydro- lH-thieno[3 ',2':4,5]benzo[ 1 ,2-b][ 1 ,4]oxazine- 7-carboxylate

Methyl 5-amino-6-hydroxybenzo[b]thiophene-2-carboxylate (85.0 mg, 0.38 mmol) was dissolved in anhydrous DMF (3.8 mL), and 1,2-dibromoethane (215.0 mg, 1.14 mmol) and K₂C0₃ (116.0 mg, 0.83 mmol) were added at room temperature. The reaction mixture was stirred at 75 °C for 15 hours, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN : 0.1 % formic acid in H₂0) to obtain methyl 2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxylate (45.2 mg, 47%) as a gray solid.

LC/MS ESI (+): 250 (M+l)

1H-NMR (400MHz, DMSO-i/₆): δ 7.89 (s, 1H), 7.26 (s, 1H), 7.07 (s, 1H), 6.16 (s, 1H), 4.19 (t, 2H, J= 4.6Hz), 3.82 (s, 3H), 3.30-3.32 (m, 2H) (f) Synthesis of methyl l-(methylsulfonyl)-2,3-dihydro-lH- thieno[3',2':4,5]benzo[l ,2-b][l ,4]oxazine-7-carboxylate

Methyl 2,3-dihydro- lH-thieno[3',2':4,5]benzo[ 1 ,2-b] [ 1 ,4]oxazine-7-carboxylate (69.0 mg, 0.27 mmol) was dissolved in CH₂C1₂ (2.7 mL), CH₃S0₂C1 (28.0 μί, 0.36 mmol) was added dropwise at 0°C. The reaction mixture was stirred at room temperature for 1 hour, H₂0 was added, and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1 % formic acid in CH₃CN : 0.1% formic acid in H₂0) to obtain methyl l-(methylsulfonyl)-2,3- dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l ,4]oxazine-7-carboxylate (42.3 mg, 46%) as a white solid.

LC/MS ESI (+): 328 (M+l)

Ή-NMR (400MHz, DMSO-rf₆): δ 8.21 (s, 1H), 8.12 (s, 1H), 7.63 (s, 1H), 4.35 (t, 2H, J= 4.3Hz), 3.86- 3.89 (m, 5H), 3.17 (s, 3H) (g) Synthesis of l-(methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2- b][l,4]oxazine-7-carboxylic acid

The synthesis procedure of Intermediate 1-g was repeated except for using methyl l-(methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l ,2-b][l,4]oxazine-7- carboxylate (42.0 mg, 0.12 mmol) to obtain l-(methylsulfonyl)-2,3-dihydro-lH- thieno[3',2':4,5]benzo[l ,2-b][l ,4]oxazine-7-carboxylic acid (35.1 mg, 87%) as a white solid.

LC/MS ESI (-): 312 (M-l)

Ή-NMR (400MHz, DMSO- ): δ 13.32 (brs, 1H), 8.18 (s, 1H), 8.00 (s, 1H), 7.60 (s, 1H), 4.36 (t, 2H, J= 4.7Hz), 3.89 (t, 2H, J=4.7Hz), 3.18 (s, 3H) Intermediate 2) Synthesis of 3,3-dimethyl-l-(methyIsulfonyI)-2,3-dihydro-l /- thieno [3 ',2 ' :4,5] benzo [ 1 ,2-b] [1,4] oxazine-7-carboxylic acid (a) Synthesis of methyl 5-(2-bromo-2-methylpropanamido)-6- hydroxybenzo[b]thiophene-2-carboxylate

Methyl 5-amino-6-hydroxybenzo[b]thiophene-2-carboxylate (110.0 mg, 0.49 mmol) was dissolved in DMA (5.0 mL), and 2-bromo-2-methyl propanoyl bromide (73.0 μί, 0.59 mmol) and DIPEA (258.0 μί, 1.47 mmol) were added. The reaction mixture was stirred at room temperature for 3 hours, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN : 0.1% formic acid in H₂0) to obtain methyl 5-(2-bromo-2-methylpropanamido)-6-hydroxybenzo[b]thiophene-2- carboxylate (161.0 mg, 88%) as a white solid.

LC/MS ESI (+): 372 (M+l)

1H-NMR (400MHz, DMSO- ): δ 11.15 (brs, 1H), 9.24 (s, 1H), 8.49 (s, 1 H), 8.08 (s, 1H), 7.43 (s, 1H), 3.85 (s, 3H), 2.03 (s, 6H) (b) Synthesis of methyl 3,3-dimethyl-2-oxo-2,3-dihydro-lH- thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxylate

Methyl 5-(2-bromo-2-methylpropanamido)-6-hydroxybenzo[b]thiophene-2- carboxylate (161.0 mg, 0.43 mmol) was dissolved in DMA (4.3 mL) and K₂C0₃ (132.0 mg, 0.95 mmol) was added. The reaction mixture was stirred at 60°C for 15 hours, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1%) formic acid in CH₃CN : 0.1% formic acid in H₂0) to obtain methyl 3,3-dimethyl-2-oxo-2,3-dihydro-lH- thieno[3',2':4,5]benzo[l,2-&][l,4]oxazine-7-carboxylate (112.0 mg, 89%) as a gray solid.

LC/MS ESI (-): 290 (M-l)

Ή-NMR (400MHz, DMSO-< ₆): δ 10.95 (s, 1H), 8.14 (s, 1H), 7.66 (s, 1H), 7.46 (s, 1H), 3.86 (s, 3H), 1.44 (s, 6H)

(c) Synthesis of methyl 3,3-dimethyl-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2- 6][l ,4]oxazine-7-carboxylate

Methyl 3,3-dimethyl-2-oxo-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2- b][l,4]oxazine-7-carboxylate (112.0 mg, 0.38 mmol) was dissolved in THF (4.0 mL), and 1M solution of BH₃-THF complex (1.9 mL, 1.92 mmol) was added at 0°C. The reaction mixture was stirred at room temperature for 3 hours, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain methyl 3,3-dimethyl-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7- carboxylate (80.0 mg, 75%) as a gray solid.

LC/MS ESI (+): 278 (M+l)

Ή-NMR (400MHz, DMSO-- ): δ 7.90 (s, 1H), 7.23 (s, 1H), 7.11 (s, 1H), 6.26 (brs, 1H), 3.83 (s, 3H) 3.06 (d, 2H, J=2.3Hz), 1.29 (s, 6H)

(d) Synthesis of methyl 3,3-dimemyl-l-(methylsulfonyl)-2,3-dihydro-lH- thieno[3',2':4,5]benzo[l,2-b][l ,4]oxazine-7-carboxylate

Methyl 3,3-dimethyl-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7- carboxylate (65.0 mg, 0.88 mmol) was dissolved in pyridine (2.3 mL) and CH₃S0₂C1 (95.0 μί, 1.06 mmol) was added dropwise at 0°C. The reaction mixture was stirred at room temperature for 15 hours, H₂0 was added, and extracted with EtO Ac. The organic extract was washed with brine, dried over anhydrous Na₂SC>4 and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (CIS- silica gel, 0.1% formic acid in C¾CN : 0.1% formic acid in H₂0) to obtain methyl 3,3- dimethyl-l-(methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7- carboxylate (52.2 mg, 63%) as a white solid.

LC/MS ESI (+): 356 (M+l)

Ή-NMR (400MHz, DMSO-rf₆): δ 8.20 (s, 1H), 8.11 (s, 1H), 7.56 (s, 1H), 3.87 (s, 3H), 3.67 (s, 2H), 3.37 (s, 3H), 1.36 (s, 6H)

(e) Synthesis of 3,3-dimethyl-l-(methylsulfonyl)-2,3-dihydro-lH- thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxylic acid

The synthesis procedure of Intermediate 1 -g was repeated except for using methyl 3,3-dimethyl-l-(methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2- b][l,4]oxazine-7-carboxylate (52.0 mg, 0.14 mmol) as a starting material to obtain 3,3- dimethyl-l-(methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-6][l,4]oxazine-7- carboxylic acid (45.0 mg, 90%).

LC/MS ESI (+): 342 (M+l)

1H-NMR (400MHz, DMSO-rf₆): δ 13.30 (s, 1H), 8.17 (s, 1H), 8.01 (s, 1H), 7.53 (s, 1H), 3.66 (s, 2H), 3.34 (s, 3H), 1.36 (s, 6H)

Intermediate 3) Synthesis of l-(methylsulfonyI)-l,2,3,4- tetrahydrothieno[3',2':4,5]benzo[l,2-b][l,4]oxazephine-8-carboxylic acid

(a) Synthesis of methyl 6-hydroxy-5-(methylsulfonamido)benzo[b]thiophene-2- carboxylate Methyl 5-amino-6-hydroxybenzo[b]thiophene-2-carboxylate (126.0 mg, 0.56 mmol) was dissolved in pyridine (2.8 mL), and CH₃S0₂C1 (50.6 μί, 0.64 mmol) was added dropwise. The reaction mixture was stirred at room temperature for 2 hours, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN : 0.1% formic acid in H₂0) to obtain methyl 6-hydroxy-5- (methylsulfonamido)benzo[b]thiophene-2-carboxylate (131.0 mg, 77%) as a white solid.

LC/MS ESI (-): 300 (M-l)

1H-NMR (400MHz, DMSO-c¾): δ 9.92 (brs, 1H), 8.08 (s, 1H), 7.85 (s, 1H), 7.43 (s,

1H), 3.86 (s, 3H), 2.99 (s, 3H)

(b) Synthesis of methyl l-(methylsulfonyl)-l,2,3,4- tetrahydrothieno[3',2':4,5]benzo[ 1 ,2-b][ 1 ,4]oxazephine-8-carboxylate

Methyl 6-hydroxy-5-(methylsulfonamido)benzo[b]thiophene-2-carboxylate (5.0 mg, 0.02 mmol) as a starting material was dissolved in DMA (160.0 μί), and 1,3- dibromopropane (16.7 mg, 0.08 mmol) and K₂C0₃ (116.0 mg, 0.83 mmol) were added. The reaction mixture was stirred at room temperature for 15 hours, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, «-Hex : EtOAc = 2 : 1) to obtain methyl 1- (methylsulfonyl)- 1 ,2,3,4-tetrahydrothieno[3',2':4,5]benzo[ 1 ,2-b][ 1 ,4]oxazephine-8- carboxylate (1.2 mg, 21%) as a white solid.

LC/MS ESI (+): 342 (M+l)

1H-NMR (400MHz, DMSO- ₆): δ 8.20 (s, 1H), 8.07 (s, 1H), 7.86 (s, 1H), 4.13-4.15

(m, 2H), 3.89 (s, 3H), 3.72-3.75 (m, 2H), 3.09 (s, 3H), 2.04-2.07 (m, 2H)

(c) Synthesis of l-(methylsulfonyl)-l,2,3,4-tetrahydrothieno[3',2':4,5]benzo[l,2- b] [ 1 ,4]oxazephine-8-carboxylic acid

The synthesis procedure of Intermediate 1-g was repeated except for using methyl l-(methylsulfonyl)-l,2,3,4-tetrahydrothieno[3',2':4,5]benzo[l,2-b][l,4]oxazephine-8- carboxylate (42.0 mg, 0.12 mmol) as a starting material to obtain l-(methylsulfonyl)- l,2,3,4-tetrahydrothieno[3',2':4,5]benzo[l,2-b][l,4]oxazephine-8-carboxylic acid (22.3 mg, 55%).

LC/MS ESI (+): 328 (M+l)

1H-NMR (400MHz, DMSO-</₆): δ 13.52 (brs, 1H), 8.09 (s, 1H), 8.04 (s, 1H), 7.83 (s, 1H), 4.12-4.14 (m, 2H), 3.73-3.75 (m, 2H), 3.09 (s, 3H), 1.99-2.07 (m, 2H)

Intermediate 4) Synthesis of 8,8-dimethyI-5-(methylsulfonyl)-5,6,7,8- tetrahydrothieno [2,3-g] quinoline-2-carboxylic acid

(a) Synthesis of ethyl 5-(3-methylbut-2-enamido)benzo[b]thiophene-2-carboxylate Ethyl 5-aminobenzo[b]thiophene-2-carboxylate (1.5 g, 6.78 mmol) was dissolved in pyridine (33.9 mL), and 3-methylbut-2-enoyl chloride (755.0 μί, 6.78 mmol) was added. The reaction mixture was stirred at room temperature for 1 hour, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, «-Hex : EtOAc = 9 : 1) to obtain ethyl 5-(3- methylbut-2-enamido)benzo[Z>]thiophene-2-carboxylate (1.3 g, 63%) as a colorless liquid.

1H-NMR (400MHz, CDC1₃): δ 8.29 (s, 1H), 7.99 (s, 1H), 7.77 (d, 1H, J=8.7Hz), 7.44 (d, 1H, J=8.6Hz), 7.17 (s, 1H), 5.74 (s, 1H), 4.40 (q, 2H, J=7.1Hz), 2.26 (s, 3H), 1.93 (s, 3H), 1.41 (t, 3H, J=7.1Hz) (b) Synthesis of ethyl 8,8-dimethyl-6-oxo-5,6,7,8-tetrahydrothieno[2,3- g]quinoline-2-carboxylate

Ethyl 5-(3-methylbut-2-enamido)benzo[b]thiophene-2-carboxylate (1.3 g, 4.29 mmol) was dissolved in CH₂C1₂ (42.9 mL), and A1C1₃ (1.7 g, 12.86 mmol) was added at 0°C. The reaction mixture was stirred at room temperature for 2 hours, H₂0 was added, and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN : 0.1%) formic acid in H₂0) to obtain ethyl 8,8-dimethyl-6-oxo-5,6,7,8-tetrahydrothieno[2,3- g]quinoline-2-carboxylate (250.0 mg, 19%) as a yellow solid.

1H-NMR (400MHz, CDC1₃): δ 8.44 (s, 1H), 8.41 (s, 1H), 7.94 (s, 1H), 7.76 (s,

1H), 4.42 (q, 2H, J=7.1Hz), 2.55 (s, 2H), 1.59 (s, 6H), 1.43 (t, 3H, J=7.1Hz)

(c) Synthesis of ethyl 8,8-dimethyl-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2- carboxylate

The synthesis procedure of Intermediate 15-c was repeated except for using ethyl

8,8-dimethyl-6-oxo-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2-carboxylate (217.0 mg, 0.72 mmol) as a starting material to obtain ethyl 8,8-dimethyl-5,6,7,8-tetrahydrothieno[2,3- g]quinoline-2-carboxylate (56.0 mg, 27%).

1H-NMR (400MHz, CDC1₃): δ 7.78 (s, 1H), 7.63 (s, 1H), 6.88 (s, 1H), 4.37 (q, 2H, J=7.1Hz), 3.37 (t, 2H, J=5.8Hz), 1.78 (t, 2H, J=5.8Hz), 1.37-1.41 (m, 9H)

(d) Synthesis of ethyl 8,8-dimethyl-5-(methylsulfonyl)-5,6,7,8- tetrahydrothieno[2,3-g]quinoline-2-carboxylate

The synthesis procedure of Intermediate 16-f was repeated except for using ethyl 8,8-dimethyl-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2-carboxylate (56.0 mg, 0.19 mmol) as a starting material to obtain ethyl 8,8-dimethyl-5-(methylsulfonyl)-5,6,7,8- tetrahydrothieno[2,3-g]quinoline-2-carboxylate (57.0 mg, 80%).

1H-NMR (400MHz, CDC1₃): 6 8.21 (s, 1H), 7.97 (s, 1H), 7.83 (s, 1H), 4.40 (q, 2H, J=7.1Hz), 3.90 (t, 2H, J=5.8Hz), 2.92 (s, 3H), 1.88 (t, 2H, J=5.8Hz), 1.39-1.43 (m, 9H)

(e) Synthesis of 8,8-dimethyl-5-(methylsulfonyl)-5,6,7,8-tetrahydrothieno[2,3- g]quinoline-2-carboxylic acid

The synthesis procedure of Intermediate 1-g was repeated except for using ethyl 8,8-dimethyl-5-(methylsulfonyl)-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2-carboxylate (57.0 mg, 0.16 mmol) as a starting material to obtain 8,8-dimethyl-5-(methylsulfonyl)- 5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2-carboxylic acid (52.0 mg, 80%).

LC/MS ESI (+): 340 (M+l)

Ή-NMR (400MHz, CDC1₃): δ 8.25 (s, 1H), 8.08 (s, 1H), 7.87 (s, 1H), 3.91 (t, 2H, J=5.8Hz), 2.94 (s, 3H), 1.89 (t, 2H, J=5.8Hz), 1.43 (s, 6H)

Intermediate 5) Synthesis of 4-(teri-butoxycarbonyl)-l-(methylsuIfonyl)- l,2,3,4-tetrahydrothieno[2,3-g]quinoxaIine-7-carboxylic acid (a) Synthesis of 4-bromo-2-fluoro-5-nitrobenzaldehyde

4-bromo-2-fluorobenzaldehyde (2.0 g, 9.85 mmol) was dissolved in cone. H₂SC¼ (5.2 mL, 98.50 mmol), and 60% HN0₃ (1.0 mL, 12.80 mmol) was added at 0°C. The reaction mixture was stirred for 4 hours, and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was recrystallized using z^'-Pr₂0 to obtain 4-bromo-2-fluoro-5- nitrobenzaldehyde (900.0 mg, 37%) as a white solid.

Ή-NMR (400MHz, CDC1₃): δ 10.31 (s, 1H), 8.42 (d, 1H, J=6.4Hz), 7.67 (d, 1H, J=9.0Hz). (b) Synthesis of methyl 6-bromo-5-nitrobenzo[b]thiophene-2-carboxylate

4-bromo-2-fluoro-5-nitrobenzaldehyde (5.0 g, 20.10 mmol) was dissolved in anhydrous DMF (50.0 mL), and methyl 2-mercaptoacetate (2.1 g, 20.10 mmol) and K₂C0₃ (5.6 g, 40.30 mmol) were added, followed by heating at 80°C for 2 hours. The reaction mixture was cooled to room temperature and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 6-bromo-5- nitrobenzo[Z>]thiophene-2-carboxylate (3.4 g, 53%) as a yellow solid.

1H-NMR (400MHz, DMSO-<4): δ 8.38 (s, 1H), 8.23 (s, 1H), 8.09 (s, 1H), 3.98 (s, 3H) (c) Synthesis of methyl 6-amino-5-nitrobenzo[b]thiophene-2-carboxylate

6-bromo-5-nitrobenzo[b]thiophene-2-carboxylate (3.0 g, 9.49 mmol) was dissolved in DMSO (10.0 ml) and Cu₂0 (830.0 mg, 10.40 mmol), sodium azide (1.2 g, 18.90 mmol) were added at room temperature. The mixture was stirred at 100 °C for 1 hour and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain methyl 6-amino-5-nitrobenzo[b]thiophene-2-carboxylate (1.6 g, 66%). as an off-white solid.

LC/MS ESI (+): 253 (M+l)

1H-NMR(400MHz, CDC1₃): δ 8.77 (s, 1H), 8.11 (s, 1H), 7.44 (s, 1H), 7.39 (s, 2H), 3.33 (s, 3H)

(d) Synthesis of methyl 6-((teri-butoxycarbonyl)amino)-5-nitrobenzo[b]thiophene- 2-carboxylate

Methyl 6-amino-5-nitrobenzo[b]thiophene-2-carboxylate (800.0 mg, 3.17 mmol) was dissolved in DMA (10.0 mL) and Boc₂0 (831.0 mg, 3.81 mmol), DIPEA (1.6 mL, 9.51 mmol) were added at room temperature. The mixture was stirred at 100 °C for 2 hours and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain methyl 6-((tert-butoxycarbonyl)amino)-5- nitrobenzo[b]thiophene-2-carboxylate (663.0 mg, 59%), as an off-white solid.

'H-NMR(400MHz, CDCI₃): δ 9.59 (s, 1H), 8.74 (s, 1H), 8.32 (s, 1H), 8.31 (s, 1H), 3.91 (s, 3H), 1.46 (s, 9H)

(e) Synthesis of methyl 5-amino-6-((tert- butoxycarbonyl)amino)benzo[b]thiophene-2-carboxylate

Methyl 6-((tert-butoxycarbonyl)amino)-5-nitrobenzo[b]thiophene-2-carboxylate (662.0 mg, 1.87 mmol) was dissolved in a mixture solvent of MeOH/H₂0 (20.0 mL, 9/1 v/v), and Zn (18.7 g, 18.70 mmol) and NH₄C1 (1.0 g, 18.70 mmol) were added thereto, and an ultrasonic reaction was conducted at 30°C for 15 hours. The reaction mixture was filtered through Celite and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain methyl 5-amino-6-((tert-butoxycarbonyl) amino) benzo [b]thiophene-2-carboxylate (633.0 mg, 104%) as an off-white solid.

LC/MS ESI (+): 323 (M+l)

1H-NMR(400MHz, CDC1₃): δ 8.56 (s, 1H), 8.01 (s, 1H), 7.92 (s, 1H), 7.21 (s, 1H), 5.14 (s, 2H), 3.85 (s, 3H), 1.50 (s, 9H) (f) Synthesis of methyl 6-((tert-butoxycarbonyl)amino)-5-(methylsulfonamido) benzo[b]thiophene-2-carboxylate

Methyl 5-amino-6-((/ert-butoxycarbonyl) amino) benzo [b]thiophene-2- carboxylate (633.0 mg, 1.96 mmol) was dissolved in pyridine (9.8 mL), and methanesulfonyl chloride (168.0 μί, 2.16 mmol) was slowly added thereto at 0°C. The reaction mixture was warmed to room temperature, followed by stirred for 3 hours and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed- phase column chromatography (C18-silica gel, 0.1% formic acid in C¾CN: 0.1 % formic acid in H₂0) to obtain methyl 6-((/ert-butoxycarbonyl)amino)-5- (methylsulfonamido)benzo[b]thiophene-2-carboxylate (694.0 mg, 88%), as an off-white solid.

LC/MS ESI (-): 399 (M-l)

Ή-NMR (400MHz, DMSO-<¾: δ 9.28 (s, 1H), 8.55 (s, 1H), 8.41 (s, 1H), 8.16 (s, 1H), 7.99 (s, 1H), 3.89 (s, 3H), 3.02 (s, 3H), 1.51 (s, 9H)

(g) Synthesis of 4-(tert-butyl) 7-methyl l-(methylsulfonyl)-2,3- dihydrothieno [2 , 3 -g] quinoxaline-4, 7( 1 H)-dicarboxylate

Methyl 6-((iert-butoxycarbonyl)amino)-5-(methylsulfonamido) benzo[b]thiophene-2-carboxylate (684.0 mg, 1.70 mmol) was dissolved in DMA (17.1 mL), and 1,2-dibromoethane (963.0 mg, 5.12 mmol) and K₂C0₃ (472.0 mg, 3.42 mmol) were slowly added at room temperature. The mixture was stirred for 1 hour, and the reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 4-(tert-butyl) 7-methyl l-(methylsulfonyl)-2,3- dihydrothieno[2,3-g]quinoxaline-4,7(lH)-dicarboxylate (495.0 mg, 68%), as an off-white solid.

Ή-NMR (400MHz, DMSO-i¾: δ 8.42 (s, 1H), 8.17 (s, 1H), 8.12 (s, 1H), 3.85- 3.92 (m, 4H), 3.89 (s, 3H), 3.12 (s, 3H), 1.51 (s, 9H)

(h) Synthesis of 4-(tert-butoxycarbonyl)-l-(methylsulfonyl)-l,2,3,4- tetrahydrothieno[2,3-g]quinoxaline-7-carboxylic acid

4-(tert-butyl) 7-methyl 1 -(methylsulfonyl)-2,3-dihydrothieno[2,3-g]quinoxaline- 4,7(lH)-dicarboxylate (495.0 mg, 1.16 mmol) was dissolved in THF/H₂0 (10.0 mL, 3/1 v/v), and LiOH-H₂0 (146.0 mg, 3.48 mmol) was added. After stirring at room temperature for 2 hours, the reaction mixture was concentrated under a reduced pressure. The residue was diluted in H₂0 (1.0 mL), and acidified with IN HC1 (pH 1-2). The precipitate was filtered to obtain 4-(tert-butoxycarbonyl)-l-(methylsulfonyl)-l ,2,3,4- tetrahydrothieno[2,3-g]quinoxaline-7-carboxylic acid (422.0 mg, 88%) as a white solid. LC/MS ESI (-): 411 (M-l)

1H-NMR (400MHz, DMSO-i¾: δ 13.47 (brs, 1H), 8.37 (s, 1H), 8.09 (s, 1H), ,8.05 (s, 1H), 3.83 ~ 3.92 (m, 4H), 3.11 (s, 3H), 1.50 (s, 9H) Intermediate 6) Synthesis of 5-(methylsulfonyl)-5,6,7,8-tetrahydrothieno[2,3- g]quinoline-2-carboxylic acid

(a) Synthesis of methyl (E)-6-(3-methoxy-3-oxoprop-l-en-l-yl)-5- nitrobenzo[b]thiophene-2-carboxylate

Methyl 6-bromo-5-nitrobenzo[b]thiophene-2-carboxylate (0.4 g, 1.26 mmol) was dissolved in anhydrous DMF(12.6 ml), and Pd(OAc)₂ (28.0 mg, 0.13 mmol), PPh₃ (66.0 mg, 0.25 mmol), TEA (0.4 ml, 2.53 mmol) and methyl acrylate (0.6 ml, 6.33 mmol) were added. The reaction mixture was stirred at 130°C for 30 minutes, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was recrystallized with diethyl ether to obtain methyl (E)-6-(3-methoxy-3-oxoprop- 1 -en- 1 -yl)-5-nitrobenzo[b]thiophene-2-carboxylate as a white solid.

LC/MS ESI (+): 322 (M+l)

1H-NMR (400MHz, CDC1₃): δ 8.62 (s, 1H), 8.16-8.22 (m, 2H), 8.09 (s, 1H), 6.43 (d, 1H, J=16.0Hz), 4.00 (s, 3H), 3.85 (s, 3H)

(b) Synthesis of methyl 6-oxo-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2- carboxylate

(E)-methyl 6-(3-methoxy-3-oxoprop-l -en-1 -yl)-5-nitrobenzo[b]thiophene-2- carboxylate (0.2 g, 0.62 mmol) was dissolved in anhydrous MeOH(15.6 mL), and 5% Pd-C (20.0 mg, 0.19 mmol) was added. The reaction mixture was charged with H₂ gas and stirred at 25°C for 4 days. The residue was filtered through Celite and concentrated under reduced pressure to obtain methyl 6-oxo-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2- carboxylate (100.0 mg, 61%) as a white solid without purification.

LC/MS ESI (+): 262 (M+l)

1H-NMR (400MHz, CDC1₃): δ 7.95 (s, 1H), 7.80 (brs, 1H), 7.66 (s, 1H), 7.21 (s, 1H), 3.94 (s, 3H), 3.11 (t, 2H, J=7.2Hz), 2.69 (t, 2H, J=7.2Hz)

(c) Synthesis of methyl 5,6,7, 8-tetrahydrothieno[2,3-g]quinoline-2-carboxylate Methyl 6-oxo-5,6,7,8-tetrahydrothieno[2,3-g-]quinoline-2-carboxylate (100.0 mg,

0.38 mmol) was dissolved in THF (4.0 mL), and 1M solution of BH₃-THF complex (1.9 mL, 1.91 mmol) was added at 0°C. The reaction mixture was stirred at room temperature for 3 hours, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain methyl 5,6,7,8- tetrahydrothieno[2,3-g]quinoline-2-carboxylate (65.0 mg, 69%) as a gray solid.

LC/MS ESI (+): 248 (M+l)

1H-NMR (400MHz, CDC1₃): δ 7.80 (s, 1H), 7.40 (s, 1H), 6.89 (s, 1H), 3.91 (s, 3H), 3.35 (t, 2H, J=5.3Hz), 2.92 (t, 2H, J=6.3Hz), 1.96-1.99 (m, 2H)

(d) Synthesis of methyl 5-(methylsulfonyl)-5,6,7,8-tetrahydrothieno[2,3- g] quinoline-2-carboxylate

Methyl 5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2-carboxylatee (50.0 mg, 0.20 mmol) was dissolved in CH₂C1₂ (2.0 mL), and CH₃S0₂C1 (23.6 μϋ, 0.30 mmol) and DIPEA (70.6 μΕ, 0.40 mmol) were added at 0°C. The reaction mixture was stirred for 3 hours and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under a reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = 2 : 1) to obtain methyl 5- (methylsulfonyl)-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2-carboxylate (57.0 mg, 87%) as a white solid.

LC/MS ESI (+): 326 (M+l)

1H-NMR (400MHz, CDC1₃): δ 8.21 (s, 1H), 7.99 (s, 1H), 7.63 (s, 1H), 3.94 (s, 3H), 3.87 (t, 2H, J=6.0Hz), 2.99 (t, 2H, J=6.0Hz), 2.90 (s, 3H), 2.04-2.07 (m, 2H)

(e) Synthesis of 5-(methylsulfonyl)-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2- carboxylic acid

The synthesis procedure of Intermediate 5-h was repeated except for using methyl 5-(methylsulfonyl)-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2-carboxylate(60.0 mg, 0.18 mmol) to obtain 5-(methylsulfonyl)-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2-carboxylic acid (450.0 mg, 87 %), as a white solid.

LC/MS ESI (+): 312 (M+l)

Intermediate 7) Synthesis of 4-methyl-l-(methylsulfonyl)-l,2,3,4- tetrahydrothieno[2,3-g]quinoxaline-7-carboxylic acid

(a) Synthesis of methyl l-(methylsulfonyl)-l,2,3,4-tetrahydrothieno[2,3- g] quinoxaline-7-carboxylate

Unpurified tert-butyl 7-(chlorocarbonyl)-l-(methylsulfonyl)-2,3- dihydrothieno[2,3-g]quinoxaline-4(lH)-carboxylate (40.0 mg, 0.09 mmol) was dissolved in CH₂Cl₂/MeOH (1.6 mL, 1/1 v/v), and TFA (0.3 mL) was slowly added. After stirring at room temperature for 2 hours, the reaction mixture was concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain methyl 1- (methylsulfonyl)-l,2,3,4-tetrahydrothieno[2,3-g]quinoxaline-7-carboxylate (21.0 mg, 69%) as a white solid.

LC/MS ESI (+): 327 (M+l)

1H-NMR (400MHz, DMSO- ₆): δ 7.97 (s, 1H), 7.90 (s, 1H), 7.09 (s, 1H), 7.00 (s, 1H), 3.82 (s, 3H), 3.67-3.69 (m, 2H), 3.43-3.45 (m, 2H), 3.00 (s, 3H)

(b) Synthesis of methyl 4-methyl-l-(mefhylsulfonyl)- 1,2,3,4- tetrahydrothieno[2,3-g]quinoxaline-7-carboxylate

Methyl 1 -(methylsulfonyl)-l ,2,3,4-tetrahydrothieno[2,3-g]quinoxaline-7- carboxylate (63.0 mg, 0.19 mmol) was dissolved in MeOH (1.9 mL), and formaldehyde (76.0 μί, 0.96 mmol) and sodium cyanoborohydride (36.4 mg, 0.57 mmol) and AcOH (11.0 μΐ,, 0.19 mmol) were added thereto at room temperature. The mixture was stirred for 15 hours, and the reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, filtered and concentrated under a reduced pressure. The residue was separated on reversed-phase silica by column chromatography (0.1% formic acid in CH₃CN : 0.1% formic acid in H₂0 = 70 : 30), and fractions including the product were combined and evaporated to obtain methyl 4-methyl- l-(methylsulfonyl)-l,2,3,4-tetrahydrothieno[2,3-g]quinoxaline-7-carboxylate (51.0 mg, 78%) as a white solid.

LC/MS ESI (+): 341 (M+l)

1H-NMR (400MHz, DMSO-i/₆): δ 8.00 (s, 1H), 7.90 (s, 1H), 7.28 (s, 1H), 3.84 (s, 3H), 3.76 (t, 2H, J=5.3Hz), 3.51 (t, 2H, J=5.3Hz), 3.02 (s, 3H), 3.01 (s, 3H)

(c) Synthesis of 4-methyl-l-(methylsulfonyl)-l,2,3,4-tetrahydrothieno[2,3- g]quinoxaline-7-carboxylic acid

Methyl 4-methyl-l -(methylsulfonyl)-l ,2,3,4-tetrahydrothieno[2,3-g]quinoxaline- 7-carboxylate (50.0 mg, 0.14 mmol) was dissolved in THF/H₂0 (1.5 mL, 3/1 v/v), and LiOH H₂0 (18.4 mg, 0.44 mmol) was added thereto. After stirring at room temperature for 2 hours, the reaction mixture was concentrated under a reduced pressure. The residue was diluted in H₂0 (1.0 mL), and acidified with IN HC1 (pH 1-2). The precipitate was filtered to obtain 4-methyl-l-(methylsulfonyl)-l,2,3,4-tetrahydrothieno[2,3- g]quinoxaline-7-carboxylic acid (42.0 mg, 88%) as a white solid.

LC/MS ESI (+): 327 (M+l)

1H-NMR (400MHz, DMSO-c?₆): δ 13.02 (brs, 1H), 7.89 (s, 1H), 7.87 (s, 1H), 7.26 (s, 1H), 3.76 (t, 2H, J=5.4Hz), 3.50 (t, 2H, J=5.4Hz), 3.01 (s, 3H), 3.00 (s, 3H)

Intermediate 8) Synthesis of 4-(methylsulfonyl)-3,4-dihydro-2/-^r-thieno[3,2- g]chromene-7-carboxylic acid

(a) Synthesis of 2-chloro-4-(3,3-diethoxypropoxy)-l-methylbenzene

3-Chloro-4-methylphenol (3.0 g, 21.04 mmol) was dissolved in anhydrous DMF (105.0 mL), and 3-chloro-l ,l-diethoxypropane (4.2 g, 25.20 mmol) and K₂C0₃ (8.7 g, 63.10 mmol) were added. The reaction mixture was stirred at 80°C for 15 hours, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = 95 : 5) to obtain 2-chloro-4- (3,3-diethoxypropoxy)-l-methylbenzene (4.0 g, 69%) as a colorless liquid.

1H-NMR (400MHz, CDC1₃): δ 7.16 (d, 1H, J=8.4Hz), 6.91 (d, 1H, J=2.6Hz), 6.71 (dd, 1H, J=8.4Hz, 2.6Hz), 4.75 (t, 1H, J=5.7Hz), 4.1 (t, 2H, J=6.3Hz), 3.66-3.73 (m, 2H), 3.49-3.57 (m, 2H), 2.29 (s, 3H), 2.05-2.10 (m, 2H), 1.22 (t, 6H, J=7.1Hz).

(b) Synthesis of 7-chloro-6-methyl-4-(methylsulfonyl)chromane

Sodium methane sulfinate (4.5 g, 44.00 mmol) was dissolved in TFA (61.1 mL) and stirred at 0°C for 10 minutes. A solution of 2-chloro-4-(3,3-diethxypropoxy)-l- methylbenzene (4.0 g, 14.66 mmol) in CH₂C1₂ (12.2 mL) was added to the reaction mixture for a period of 1 hour, and stirred at room temperature for 30 minutes. The reaction mixture was basified with sat. NaHC0₃ aqueous solution (pH=9-10), and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = 1 : 2) to obtain 7-chloro-6-methyl-4- (methylsulfonyl)chromane (2.3 g, 60%) as an off-white solid.

1H-NMR (400MHz, CDC1₃): δ 7.36 (s, 1H), 6.95 (s, 1H), 4.40-4.46 (m, 1H), 4.17- 4.25 (m, 2H), 2.83 (s, 3H), 2.55-2.62 (m, 1H), 2.32-2.42 (m, 1H), 2.31 (s, 3H).

(c) Synthesis of 6-(bromomethyl)-7-chloro-4-(methylsulfonyl)chromane

7-Chloro-6-methyl-4-(methylsulfonyl)chromane (2.3 g, 8.63 mmol) was dissolved in anhydrous 1 ,2-dichloroethane (86.0 mL), and N-bromosuccinimide (1.5 g, 8.63 mmol) and AIBN (142.0 mg, 0.86 mmol) were added. The reaction mixture was refluxed at 100°C for 15 hours, cooled to room temperature, H₂0 was added, and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = 4 : 1) to obtain 6-(bromomethyl)-7-chloro-4- (methylsulfonyl)chromane (2.5 g, 85%) as a yellow solid.

Ή-NMR (400MHz, CDC1₃): δ 7.59 (s, 1H), 7.00 (s, 1H), 4.47-4.62 (m, 3H), 4.25- 4.31 (m, 1H), 4.19-4.22 (m, 1H), 2.87 (s, 3H), 2.56-2.62 (m, 1H), 2.34-2.43 (m, 1H).

(d) Synthesis of 7-chloro-4-(methylsulfonyl)chromane-6-carbaldehyde

6-(Bromomethyl)-7-chloro-4-(methylsulfonyl)chromane (2.5 g, 7.36 mmol) was dissolved in anhydrous CH₃CN (73.6 mL) , and 4-methylmorpholine N-oxide (1.7 g, 14.72 mmol) and a molecular sieve (3.0 g) were added. The reaction mixture was stirred at room temperature for 2 hours, H₂0 was added to the reaction mixture, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was recrystallized with Et₂0 to obtain 7- chloro-4-(methylsulfonyl)chromane-6-carbaldehyde (1.3 g, 64%) as a pale yellow solid.

LC/MS ESI (+): 275 (M+l).

Ή-NMR (400MHz, CDC1₃): δ 10.32 (s, 1H), 7.97 (s, 1H), 7.03 (s, 1H), 4.64-4.71 (m, 1H), 4.39-4.44 (m, 1H), 4.23 (m, 1H), 2.99 (s, 3H), 2.73-2.78 (m, 1H), 2.30-2.34 (m, 1H).

(e) Synthesis of methyl 4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxylate

7-Chloro-4-(methylsulfonyl)chromane-6-carbaldehyde (300.0 mg, 1.09 mmol) was dissolved in anhydrous DMF (10.0 mL), and methyl 2-mercapto acetate (117.0 μί, 1.31 mmol) and Cs₂C0₃ (1.1 g, 3.28 mmol) were added. The reaction mixture was stirred at 80°C for 4 hours, cooled to room temperature, H₂0 was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was recrystallized with CH₂C1₂ and Et₂0 to obtain methyl 4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (200.0 mg, 56%) as a white solid.

1H-NMR (400MHz, DMSO- ): δ 8.17 (s, 1H), 8.09 (s, 1H), 7.57 (s, 1H), 4.82 (m, 1H), 4.45-4.50 (m, 1H), 4.31-4.35 (m, 1H), 3.88 (s, 3H), 3.17 (s, 3H), 2.59-2.69 (m, 1H), 2.26-2.37 (m, 1H).

LC/MS ESI (+): 327 (M+l).

(f) Synthesis of 4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylic acid

Methyl 4-(methylsulfonyl)-3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxylate (200.0 mg, 0.61 mmol) was dissolved in THF (4.0 mL)/H₂0 (2.0 mL), and LiOH H₂0 (257.0 mg, 6.13 mmol) was added. The reaction mixture was stirred at 60°C for 15 hours, IN HC1 (3.0 mL) was added, and extracted with CH₂C1₂ and MeOH. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure to obtain 4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylic acid (140.0 mg, 73%) as an off-white solid.

LC/MS ESI (-): 311 (M-l).

Ή-NMR (400MHz, DMSO-<¾: δ 13.34 (bs, 1H), 8.06 (s, 2H), 7.54 (s, 1H), 4.82 (m, 1H), 4.45-4.54 (m, 1H), 4.30-4.34 (m, 1H), 3.16 (s, 3H), 2.60-2.74 (m, 1H), 2.28-2.39 (m, 1H).

Intermediate 9) Synthesis of 5-(methylsulfonyl)-2,3,4,5- tetrahydrothieno[3',2':4,5]benzo[l,2-6]oxepine-8-carboxylic acid (a) Synthesis of 2-chloro-4-(4,4-diethoxybutoxy)-l-methylbenzene

The synthesis procedure of Intermediate 8-a was repeated except for using 3- chloro-4-methylphenol (1.0 g, 7.01 mmol) to obtain 2-chloro-4-(4,4-diethoxybutoxy)-l- methylbenzene (1.8 g, 89 %) as a colorless liquid.

LC/MS ESI (+): 287 (M+l)

Ή-NMR (400MHz, CDC1₃): δ 7.09 (d, IH, J=8.0Hz), 6.89 (d, IH, J=2.8Hz), 6.69

(dd, IH, J=8.6, 2.8Hz), 4.54 (t, IH, J=1.6Hz), 3.93 (t, 2H, J=6.0Hz), 3.62-3.70 (m, 2H),

3.47-3.54 (m, 2H), 2.28 (s, 3H), 1.74-1.87 (m, 4H), 1.21 (t, 6H, J=7.2Hz) (b) Synthesis of ^; 8-chloro-7-methyl-5-(methylsulfonyl)-2,3,4,5- tetrahydrobenzo[6]oxepine

The synthesis procedure of Intermediate 8-b was repeated except for using 2- chloro-4-(4,4-diethoxybutoxy)-l-methylbenzene (1.8 g, 6.20 mmol) to obtain 8-chloro-7- methyl-5-(methylsulfonyl)-2,3,4,5-tetrahydrobenzo[b]oxepine (421.0 mg, 23%) as a white solid.

LC/MS ESI (+): 275 (M+l)

Ή-NMR (400MHz, CDC1₃): δ 7.20 (s, IH), 7.10 (s, IH), 4.34-4.39 (m, IH), 4.16 (t, IH, J=1.6Hz), 3.71-3.77 (m, IH), 2.78 (s, 3H), 2.64-2.70 (m, IH), 2.33 (s, 3H), 2.22- 2.29 (m, IH), 2.07-2.15 (m, IH), 1.82-1.89 (m, IH).

(c) Synthesis of 7-(bromomethyl)-8-chloro-5-(methylsulfonyl)-2,3,4,5- tetrahydrobenzo[b]oxepine

The synthesis procedure of Intermediate 8-c was repeated except for using 8- chloro-7-methyl-5-(methylsulfonyl)-2,3,4,5-tetrahydrobenzo[6]oxepine (421 mg, 1.53 mmol) to obtain 7-(bromomethyl)-8-chloro-5-(methylsulfonyl)-2,3,4,5- tetrahydrobenzo[b]oxepine (342.0 mg, 63%) as a white solid.

LC/MS ESI (+): 353 (M+l)

Ή-NMR (400MHz, CDC1₃): δ 7.42 (s, IH), 7.15 (s, IH), 4.44 (m, 2H), 4.39-4.44 (m, IH), 4.20 (t, IH, J=1.6Hz), 3.76-3.82 (m, IH), 2.78 (s, 3H), 2.68-2.72 (m, IH), 2.28- 2.38 (m, IH), 2.09-2.18 (m, IH), 1.86-1.94 (m, IH)

(d) Synthesis of 8-chloro-5-(methylsulfonyl)-2,3,4,5-tetrahydrobenzo[b]oxepine-7- carbaldehyde

The synthesis procedure of Intermediate 8-d was repeated except for using 7- (bromomethyl)-8-chloro-5-(methylsulfonyl)-2,3,4,5-tetrahydrobenzo[b]oxepine (342. Omg, 0.97 mmol) to obtain 8-chloro-5-(methylsulfonyl)-2,3,4,5-tetrahydrobenzo[b]oxepine-7- carbaldehyde (164.0 mg, 57%), as a white solid.

LC/MS ESI (+): 289 (M+1)

Ή-NMR (400MHz, CDC1₃): δ 10.36 (s, IH), 7.89 (s, IH), 7.21 (s, IH), 4.55-4.59 (m, IH), 4.31 (t, IH, J=1.6Hz), 3.78-3.84 (m, IH), 2.79-2.84 (m, 4H), 2.42-2.53 (m, IH), 2.02-2.11 (m, 1H), 1.91-1.96 (m, 1H).

(e) Synthesis of 5-(methylsulfonyl)-2,3,4,5-tetrahydrothieno[3',2':4,5]benzo[l,2- £]oxepine-8-carboxylic acid

The synthesis procedure of Intermediate 8-e was repeated except for using 8- chloro-5-(methylsulfonyl)-2,3,4,5-tetrahydrobenzo[ )]oxepine-7-carbaldehyde (164.0 mg, 0.57 mmol) to obtain 5-(methylsulfonyl)-2,3,4,5-tetrahydrothieno[3',2':4,5]benzo[l,2- b]oxepine-8-carboxylic acid (116.0 mg, 62 %) as a white solid.

LC/MS ESI (+): 341 (M+l)

Ή-NMR (400MHz, DMSO-cfe): δ 13.48 (s, 1H), 8.07 (s, 1H), 8.05 (s, 1H), 7.73 (s,

1H), 4.80 (t, 1H, J=1.2 Hz), 4.33-4.39 (m, 1H), 3.75-3.80 (m, 1H), 2.89 (s, 3H), 2.50-2.53 (m, 1H) 2.18-2.27 (m, 1H), 2.06-2.14 (m, 1H), 1.75-1.88 (m, 1H)

Intermediate 10) Synthesis of 5-(methylsulfonyl)-5,6,7,8- tetrahydronaphtho[2,3-*]thiophene-2-carboxylic acid

(a) Synthesis of 2-ethylhexyl 3-((5-bromo-2-formylphenyl)thio)propanoate

To a solution of 4-bromo-2-fluorobenzaldehyde (3.0 g, 14.78 mmol) in DMF (73.9 ml) were added 2-Ethylhexyl 3-mercaptopropionate (3.7 ml, 16.26 mmol). The reaction mixture was stirred at 40°C for 36hours. The reaction mixture was purified by reversed- phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 2-ethylhexyl 3-((5-bromo-2-formylphenyl)thio)propanoate (4.2 g, 71 %) as a light brown oil.

LC/MS ESI (+): 401 (M+l)

1H-NMR (400MHz, CDC1₃): δ 10.28 (s, 1H), 7.70 (d, 1H, J=8.2Hz), 7.57 (d, 1H,

J=1.7Hz), 7.46 (dd, 1H, J=8.2, 1.7Hz), 4.03-4.05 (m, 2H), 3.24 (t, 2H, J=7.4Hz), 2.72 (t, 2H, J=7.4Hz), 1.32-1.40 (m, 3H), 1.26-1.30 (m, 6H), 0.90 (t, 6H, J=7.4Hz)

(b) Synthesis of 2-ethylhexyl 3-((5-(4,4-diethoxybutyl)-2- (hydroxymethyl)phenyl)thio)propanoate

A solution of 4,4-diethoxybut-l-ene (503.0 mg, 3.49 mmol) in 9- borabicyclo(3.3.1)nonane (7.7 ml, 3.84 mmol) was stirred for lhour and concentrated. The residue was dissolved in benzene (7.7 ml)/EtOH (3.9 ml). 2N Na₂C0₃ aqueous solution (35.0 ml, 6.98 mmol) and Pd(PPh₃)₄ (202.0 mg, 0.17 mmol) were added and the reaction mixture at 80°C for 90minutes. The reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, filtered and concentrated. The residue was purified by normal phase column chromatography (EtOAc:n-Hex=l :15) to give 2-ethylhexyl 3-((5-(4,4-diethoxybutyl)-2- formylphenyl)thio)propanoate (695.0 mg, 85 %) as crude. To a solution of 2-ethylhexyl 3- ((5-(4,4-diethoxybutyl)-2-formylphenyl)thio)propanoate (610.0 mg, 1.31 mmol) in EtOH (6.5 ml) was added NaBH₄ (64.3 mg, 1.70 mmol). The reaction mixture was stirred for 30 minutes. NH₄C1 was added, and the resulting mixture was stirred for 15 minutes and filtered through celite. The filtrate was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, and concentrated. The residue was purified by normal phase column chromatography (EtOAc:«-Hex=l :3) to give 2- ethylhexyl 3-((5-(4,4-diethoxybutyl)-2-(hydroxymethyl)phenyl)thio)propanoate (550.0 mg, 90 %) as a colorless oil.

1H-NMR (400MHz, CDC1₃): δ 7.31 (d, 1H, J=7.7Hz), 7.24 (d, 1H, J=1.7Hz), 7.07 (d, 1H, J=7.7Hz), 4.74 (d, 2H, J=3.3Hz ), 4.49 (t, 1H, J=5.2Hz), 4.00-4.02 (m, 2H), 3.59- 3.67 (m, 2H), 3.44-3.52 (m, 2H), 3.16 (t, 2H, J=7.2Hz), 2.61 (t, 4H, J=7.1Hz), 2.39 (brs, 1H), 1.65-1.68 (m, 4H), 1.51-1.57 (m, 2H), 1.26-1.37 (m, 2H), 1.26-1.31 (m, 4H), 1.20 (t, 6H, J=7.1Hz), 0.89 (t, 6H, J=7.1Hz)

(c) Synthesis of 2-ethylhexyl 3-((5-(methylsulfonyl)-3-((2,2,2- trifluoroacetoxy)methyl)-5,6,7,8-tetrahydronaphthalen-2-yl)thio)propanoate

Sodium methanesulfinate (359.0 mg, 3.52 mmol) was dissolved in TFA (9.8 ml) and the reaction mixture was stirred for 10 minutes. After cooling to 0°C, a solution of 2- ethylhexyl 3-((5-(4,4-diethoxybutyl)-2-(hydroxymethyl)phenyl)thio)propanoate (550.0 mg, 1.17 mmol) in CH₂C1₂ (1956.0 μΐ) was added dropwise and the reaction mixture was stirred at 23 °C for lhour. The reaction mixture was extracted with EtOAc, and the organic extract was washed with sat. NaHC0₃ aqueous solution and brine, dried over anhydrous Na₂S0₄, filtered and concentrated. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 3-((5-(methylsulfonyl)-3-((2,2,2-trifluoroacetoxy)methyl)-5, 6,7,8- tetrahydronaphthalen-2-yl)thio)propanoate (217.0 mg, 34 %) as a colorless oil.

1H-NMR (400MHz, CDC1₃): δ 7.63 (s, 1H), 7.26 (s, 1H), 5.45 (q, 2H, J=20.8Hz), 4.28 (t, 1H, J=5.1Hz), 4.01-4.04 (m, 2H), 3.20 (t, 2H, J=7.5Hz), 2.87-2.93 (m, 1H), 2.76- 2.81 (m, 4H), 2.65 (t, 2H, J=7.6Hz), 2.47-2.52 (m, 1H), 2.19-2.23 (m, 2H), 1.75-1.86 (m, 1H), 1.56-1.58 (m, 1H), 1.29-1.39 (m, 8H), 0.89 (t, 6H, J=7.4Hz)

(d) Synthesis of tert-butyl 2-((3-(hydroxymethyl)-5-(methylsulfonyl)-5, 6,7,8- tetrahydronaphthalen-2-yl)thio)acetate

To a solution of 2-ethylhexyl 3-((5-(methylsulfonyl)-3-((2,2,2- trifluoroacetoxy)methyl)-5,6,7,8-tetrahydronaphthalen-2-yl)thio)propanoate (150.0 mg, 0.27 mmol) in DMF (2714.0 μΐ) was added 1M solution of potassium-t-butoxide in THF (543.0 μΐ, 0.54 mmol) at -60~-50°C. The reaction mixture was stirred for lOminutes. tert- butyl 2-bromoacetate (42.1 μΐ, 0.29 mmol) was added at -60— 50°C and the reaction mixture was stirred at room temperature for lhour. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain tert-butyl 2-((3-(hydroxymethyl)-5-(methylsulfonyl)-5, 6,7,8- tetrahydronaphthalen-2-yl)thio)acetate (67.0 mg, 64 %) as a white amorphous.

1H-NMR (400MHz, CDC1₃): δ 7.60 (s, 1H), 7.26 (s, 1H), 4.77 (q, 2H, J=32.7Hz), 4.27 (t, 1H, J=5.9Hz), 3.60 (s, 2H), 2.83-2.89 (m, 1H), 2.71-2.79 (m, 4H), 2.49-2.55 (m, 1H), 2.15-2.20 (m, 2H), 1.73-1.46 (m, 1H), 1.40 (s, 9H)

(e) Synthesis of fert-butyl 2-((3-formyl-5-(methylsulfonyl)-5, 6,7,8- tetrahydronaphthalen-2-yl)thio)acetate

To a solution of tert-butyl 2-((3-(hydroxymethyl)-5-(methylsulfonyl)-5, 6,7,8- tetrahydronaphthalen-2-yl)thio)acetate (65.0 mg, 0.17 mmol) in CH₂C1₂ (1682.0 μΐ) was added Dess-Martin periodinane (107.0 mg, 0.25 mmol). The reaction mixture was stirred at 24°C for lhour. The reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, filtered and concentrated to give tert- butyl 2-((3-formyl-5-(methylsulfonyl)-5,6,7,8-tetrahydronaphthalen-2-yl)thio)acetate (71.0 mg, 110 %) as crude. The crude was used the next step without purification.

1H-NMR (400MHz, CDC1₃): δ 10.23 (s, 1H), 8.04 (s, 1H), 7.29 (s, 1H), 4.31-4.34

(m, 1H), 3.63 (s, 2H), 2.92-2.99 (m, 1H), 2.80-2.87 (m, 4H), 2.52-2.57 (m, 1H), 2.22-2.25 (m, 2H), 1.77-1.82 (m, lH), 1.44 (s, 9H)

(f) Synthesis of tert-butyl 5-(methylsulfonyl)-5,6,7,8-tetrahydronaphtho[2,3- b]thiophene-2-carboxylate

To a solution of tert-butyl 2-((3-formyl-5-(methylsulfonyl)-5,6,7,8- tetrahydronaphthalen-2-yl)thio)acetate (67.0 mg, 0.17 mmol) in DMF (1742.0 μΐ) was added Cs₂C0₃ (85.0 mg, 0.26 mmol). The reaction mixture was stirred at 80°C for 1 hour. The reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, filtered and concentrated to give tert-butyl 5- (methylsulfonyl)-5,6,7,8-tetrahydronaphtho[2,3-b]thiophene-2-carboxylate (65.0 mg, 102 %) as crude. The crude was used the next step without purification.

Ή-NMR (400MHz, CDC1₃): δ 8.11 (s, 1H), 7.93 (s, 1H), 7.66 (s, 1H), 4.43-4.46 (m, 1H), 3.00-3.08 (m, 1H), 2.90-2.98 (m, 4H), 2.53-2.59 (m, 1H), 2.31-2.35 (m, 1H), 2.17-2.19 (m, 1H), 1.71-1.83 (m, 1H), 1.61 (s, 9H)

(g) Synthesis of 5-(methylsulfonyl)-5,6,7,8-tetrahydronaphtho[2,3-b]thiophene-2- carboxylic acid

To a solution of tert-butyl 5-(methylsulfonyl)-5,6,7,8-tetrahydronaphtho[2,3- b]thiophene-2-carboxylate (65.0 mg, 0.18 mmol) in CH₂C1₂ (0.5 ml) was added TFA (0.5 ml, 6.53 mmol). The reaction mixture was stirred at 24°C for 30minutes. The reaction mixture was purified by reversed-phase column chromatography (CI 8 -silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 5-(methylsulfonyl)-5,6,7,8- tetrahydronaphtho[2,3-b]thiophene-2-carboxylic acid (30.0 mg, 55 %) as a white amorphous. LC/MS ESI (-): 309 (M-l)

1H-NMR (400MHz, DMSO-c ₆): δ 8.13 (s, 1H), 8.10 (s, 1H), 7.90 (s, 1H), 4.80- 4.82 (m, 1H), 3.05 (s, 3H), 2.91-3.02 (m, 2H), 2.48-2.51 (m, 1H), 2.19-2.23 (m, 2H), 1.67- 1.72 (m, 1H)

Intermediate 11) Synthesis of 4-methyl-4-(methylsulfonyl)-3,4-dihydro-2H- thieno [3,2-g] chromene-7-carboxylic acid

(a) Synthesis of methyl 4-methyl-4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxylate

To a suspension of methyl 4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxylate (50.0 mg, 0.15 mmol) in THF (1.0 ml)/CH₃CN (1.0 ml) was added NaH (30.6 mg, 0.77 mmol). After lhour, CH₃I (0.1 ml, 1.53 mmol) was added and the reaction mixture was stirred for 3days. The reaction mixture was purified by reversed- phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1 % formic acid in H₂0) to obtain methyl 4-methyl-4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxylate (29.0 mg, 56 %) as an off-white amorphous.

LC/MS ESI (+): 341 (M+l)

1H-NMR (400MHz, DMSO- ₆): δ 8.29 (s, 1H), 8.16 (s, 1H), 7.56 (s, 1H), 4.49- 4.56 (m, 1H), 4.20-4.26 (m, 1H), 3.88 (s, 3H), 3.00 (s, 3H), 2.61-2.68 (m, 1H), 2.16-2.23 (m, 1H), 1.83 (s, 3H)

(b) Synthesis of 4-methyl-4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxylic acid

To a suspension of methyl 4-methyl-4-(methylsulfonyl)-3,4-dihydro-2H- thieno[3,2-g]chromene-7-carboxylate (27.0 mg, 0.08 mmol) in THF (529.0 μ1)/Η₂0 (264.0 μΐ) was added LiOHH₂0 (33.3 mg, 0.79 mmol). The reaction mixture was stirred at 60°C for 1 hour. The reaction mixture was concentrated and the residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 4-methyl-4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxylic acid (20.0 mg, 77 %) as a white amorphous.

LC/MS ESI (-): 325 (M-1)

Ή-NMR (400MHz, DMSO-i ₆): δ 8.22 (s, 1H), 7.96 (s, 1H), 7.49 (s, 1H), 4.47- 4.52 (m, 1H), 4.18-4.22 (m, 1H), 2.97 (s, 3H), 2.59-2.67 (m, 1H), 2.13-2.20 (m, 1H), 1.81 (s, 3H)

Intermediate 12) Synthesis of 5-(methylsuIfonyl)-5,8-dihydro-6/7-thieno[3,2- g]isochromene-2-carboxyIic acid

Synthesis of methyl 2-bromo-4-(bromomethyl)benzoate 2-Bromo-4-methylbenzoate (4.6 g, 20.08 mmol) was dissolved in anhydrous 1 ,2- dichloroethane (60.0 ml), and N-bromosuccinimide (4.3 g, 24.10 mmol) and AIBN (0.3 g, 2.01 mmol) were added at room temperature. The mixture was refluxed at 90°C for 1 hour, followed by cooling to room temperature and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain methyl 2-bromo-4- (bromomethyl)benzoate (3.0 g, 48%) as a colorless liquid.

LC/MS ESI (+): 309 (M+l)

'H-NMR(400MHz, CDCI3): δ 7.78 (d, 1H, J=8.0Hz), 7.69 (d, 1H, J=1.6Hz), 7.38

(dd, 1H, J=8.0, 1.6Hz), 4.42 (s, 2H), 3.94 (s, 3H)

(b) Synthesis of methyl 2-bromo-4-((2,2-diethoxyethoxy)methyl)benzoate

Glycolaldehyde diethyl acetal (1.6 ml, 12.14 mmol) was dissolved in anhydrous THF (50.0 ml), and NaH (0.5 g, 13.25 mmol) was added at 0°C. The mixture was stirred at 0°C for 30 minutes. Methyl 2-bromo-4-(bromomethyl)benzoate (3.4 g, 11.04 mmol) in THF (50.0 ml) was added at 0 °C. The mixture was stirred at 0°C for 3 hours and extracted with EtOAc. The organic extract was washed with water, dried over anhydrous Na₂S0₄, and concentrated under a reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = 9 : 1) to obtain methyl 2-bromo-4-((2,2- diethoxyethoxy)methyl)benzoate (1.7 g, 42%) as a colorless liquid.

1H-NMR(400MHz, CDC1₃): δ 7.78 (d, 1H, J=8.0Hz), 7.66 (d, 1H, J=1.2Hz), 7.31- 7.34 (m, 1H), 4.67 (t, 1H, J=10.4, 5.2Hz), 4.59 (s, 2H), 3.93 (s, 3H), 3.68-3.75 (m, 2H), 3.53-3.62 (m, 4H), 1.23 (t, 6H, J=14.0, 6.8Hz)

(c) Synthesis of 4-((2,2-diethoxyethoxy)methyl)-2-(methylthio)benzoic acid Methyl 2-bromo-4-((2,2-diethoxyethoxy)methyl)benzoate (480.0 mg, 1.33 mmol) was dissolved in DMF (4.0 ml), and sodium methanethiolate (466 mg, 6.64 mmol) was added at room temperature. The mixture was stirred at 80°C for 15 hours, followed by cooling to room temperature and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 4-((2,2- diethoxyethoxy)methyl)-2-(methylthio)benzoic acid (380.0 mg, 91%) as a colorless liquid LC/MS ESI (-): 313 (M-l)

1H-NMR(400MHz, CDC1₃): δ 8.10 (d, 1H, J=8.0Hz), 7.30 (s, 1H), 7.14 (dd, 1H, J=8.0, 0.8Hz), 4.70 (t, 1H, J=10.4, 5.2Hz), 4.65 (s, 2H), 3.68-3.76 (m, 2H), 3.55-3.63 (m, 4H), 2.48 (s, 3H), 1.24 (t, 6H, J=14.0, 6.8Hz) (d) Synthesis of (4-((2,2-diethoxyethoxy)methyl)-2-(methylthio)phenyl)methanol 4-((2,2-diethoxyethoxy)methyl)-2-(methylthio)benzoic acid (380.0 mg, 1.21 mmol) was dissolved in THF (12.0 ml), and l .OM solution of LiAlH4 in THF (1.2 ml, 1.20 mmol) was added at room temperature. The mixture was stirred at room temperature for 1 hour and H₂0 was added at 0°C. The reaction mixture was filtered through Celite and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain (4-((2,2-diethoxyethoxy)methyl)-2-(methylthio)phenyl)methanol (280.0 mg, 77%) as a colorless liquid.

1H-NMR(400MHz, CDC1₃): δ 7.34 (d, 1H, J=8.0Hz), 7.26 (m, 1H), 7.12-7.14 (m,

1H), 4.75 (brs, 2H), 4.66 (t, 1H, J=10.4, 5.2Hz), 4.58 (s, 2H), 3.66-3.74 (m, 2H), 3.51-3.61 (m, 4H), 2.50 (s, 3H), 2.08 (brs, 1H), 1.22 (t, 6H, J=14.0, 7.2Hz)

(e) Synthesis of (4-(methylsulfonyl)-7-(methylthio)isochroman-6-yl)methanol Sodium methanesulfinate (285.0 mg, 2.80 mmol) was dissolved in TFA (7.5 ml) and stirred at room temperature for 30 minutes. (4-((2,2-diethoxyethoxy)methyl)-2- (methylthio)phenyl)methanol (280.0 mg, 0.93 mmol) in CH₂C1₂ (1.5 ml) was slowly added at room temperature. The mixture was stirred at room temperature for 30 minutes and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under a reduced pressure. The mixture was dissolved in THF (4.0 ml)/MeOH (1.0 ml)/H₂0 (1.0 ml) and LiOHH₂0 (223.0 mg, 932.00 mmol) was added at room temperature. The mixture was stirred at room temperature for 30 minutes and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, filtered and concentrated to obtain (4-(methylsulfonyl)-7- (methylthio)isochroman-6-yl)methanol (208.0 mg, 77%)%) as a colorless liquid.

LC/MS ESI (-): 287 (M-l)

1H-NMR(400MHz, CDC1₃): δ 7.66 (s, 1H), 6.91 (s, 1H), 4.86-4.91 (m, 2H), 4.70- 4.80 (m, 3H), 3.97-4.01 (m, 1H), 3.87-3.88 (m, 1H), 2.67 (s, 3H), 2.50 (s, 3H) (f) Synthesis of 4-(methylsulfonyl)-7-(methylthio)isochromane-6-carbaldehyde

(4-(Methylsulfonyl)-7-(methylthio)isochroman-6-yl)methanol (208.0 mg, 0.72 mmol) was dissolved in CH₂C1₂ (7.0 ml) and Dess-Martin Periodinane (398.0 mg, 0.94 mmol) was added at room temperature. The mixture was stirred at room temperature for 30 minutes and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under a reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = 1 : 1) to obtain 4- (methylsulfonyl)-7-(methylthio)isochromane-6-carbaldehyde (150.0 mg, 72%) as a colorless liquid.

LC/MS ESI (+): 287 (M+l)

1H-NMR(400MHz, CDC1₃): 6 10.16 (s, 1H), 8.10 (s, 1H), 7.00 (s, 1H), 4.93-4.97 (m, 2H), 4.80-4.84 (m, 1H), 4.02-4.06 (m, 1H), 3.94-3.95 (m, 1H), 2.69 (s, 3H), 2.48 (s, 3H)

(g) Synthesis of 5-(methylsulfonyl)-5,8-dihydro-6H-thieno[3,2-g]isochromene-2- carboxylic acid

A mixture of 4-(Methylsulfonyl)-7-(methylthio)isochroman-6-carbaldehyde (150.0 mg, 0.52 mmol), magnesium oxide (21.1 mg, 0.52 mmol) and 2-chloroacetic acid (990.0 mg, 10.48 mmol) was stirred at 110°C for 16 hours and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 5- (methylsulfonyl)-5,8-dihydro-6H-thieno[3,2-g]isochromene-2-carboxylic acid (50.0 mg, 30%) as a white solid.

LC/MS ESI (-): 311 (M-l)

1H-NMR(400MHz, CDC1₃): δ 8.26 (s, 1H), 8.14 (s, 1H), 7.64 (s, 1H), 4.91-5.08

(m, 3H), 4.07-4.11 (m, 2H), 2.66 (s, 3H)

Intermediate 13) Synthesis of 4-fluoro-4-(methylsulfonyI)-3,4-dihydro-2H- thieno [3,2-g] chromene-7-carboxylic acid

(a) Synthesis of 7-chloro-4-fluoro-6-methyl-4-(methylsulfonyl)chromane

7-chloro-6-methyl-4-(methylsulfonyl)chromane (850.0 mg, 3.26 mmol) was dissolved in THF (30.0 mL), and LDA (2.6 mL, 3.91 mmol) was slowly added at -78°C and stirred for 0.5 hour. NFS (2.3 g, 7.17 mmol) was added, followed by stirring at -78°C for 3 hours. The reaction was quenched with H₂0, and the reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, and concentrated under a reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = 85 : 15) to obtain 7-chloro-4-fluoro-6- methyl-4-(methylsulfonyl)chromane (600.0 mg, 66%) as a a white solid

Ή-NMR (400MHz, DMSO- ,;): δ 7.53 (s, 1H), 6.97 (s, lH), 4.59-4.65 (m, 1H), 4.28-4.35 (m, 1H), 3.05 (s, 3H), 2.72-2.80 (m, 1H), 2.49-2.56 (m, 1H), 2.33 (s, 3H)

(b) Synthesis of 6-(bromomethyl)-7-chloro-4-fluoro-4-(methylsulfonyl)chromane The synthesis procedure of Intermediate 8-c was repeated except for using 7- chloro-4-fluoro-6-methyl-4-(methylsulfonyl)chromane (500.0 mg, 1.79 mmol) to obtain 6- (bromomethyl)-7-chloro-4-fluoro-4-(methylsulfonyl)chromane (520.0 mg, 81%).

1H-NMR (400MHz, DMSO-i¾: δ 7.75 (s, 1H), 7.01 (s, 1H), 4.67-4.68 (m, 1H), 4.56 (q, 2H, J=10.4, 21.1Hz), 4.35-4.41 (m, 1H), 3.06 (s, 3H), 2.74-2.82 (m, 1H), 2.49- 2.60 (m, 1H) (c) Synthesis of 6-(bromomethyl)-7-chloro-4-fluoro-4-(methylsulfonyl)chromane The synthesis procedure of Intermediate 8-d was repeated except for using 6-

(bromomethyl)-7-chloro-4-fluoro-4-(methylsulfonyl)chromane (400.0 mg, 1.11 mmol) to obtain 7-chloro-4-fluoro-4-(methylsulfonyl)chromane-6-carbaldehyde (225.0 mg, 68%).

LC/MS ESI (+): 293 (M+l)

1H-NMR (400MHz, DMSO-<¾: δ 10.32 (s, 1H), 8.20 (s, 1H), 7.04 (s, 1H), 4.73- 4.80 (m, 1 H), 4.49-4.55 (m, 1 H), 3.09 (s, 3H), 2.86-2.93 (m, 1 H), 2.47-2.59 (m, 1 H)

(d) Synthesis of 4-fluoro-4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxylic acid

7-chloro-4-fluoro-4-(methylsulfonyl)chromane-6-carbaldehyde (210.0 mg, 0.71 mmol) was dissolved in anhydrous DMF (7.1 mL), and methyl 2-mercaptoacetate (1 14.0 mg, 1.07 mmol) and Cs₂C0₃ (514.0 mg, 1.57 mmol) were added, followed by heating at 80°C for 2 hours. The reaction mixture was cooled to room temperature and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1 % formic acid in H₂0) to obtain 4-fluoro-4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylic acid (72.0 mg, 30%) as a yellow solid

LC/MS ESI (-): 329 (M-l)

1H-NMR (400MHz, DMSO-<¾: δ 13.47 (s, 1H), 8.32 (s, 1H), 8.13 (s, 1H), 7.66 (s, 1H), 4.53-4.56 (m, 1H), 4.40-4.44 (m, 2H), 2.95-2.97 (m, 2H), 2.64-2.67 (m, 2H)

Intermediate 14) Synthesis of 8,8-difluoro-5-(methylsulfonyI)-5,6,7,8- tetrahydronaphtho [2,3-6] thiophene-2-carboxylic acid

(a) Synthesis of 7-chloro-6-methyl-4-(methylsulfonyl)-3,4-dihydronaphthalen- l (2H)-one

To a solution of 6-chloro-7-methyl-l-(methylsulfonyl)-l , 2,3,4- tetrahydronaphthalene (700.0 mg, 2.71 mmol) in acetic anhydride (51 14.0 μΐ, 54.10 mmol) was added chromium oxide(VI) (812.0 mg, 8.12 mmol) at 0°C. The reaction mixture was stirred at 0°C for 2hours. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1 % formic acid in CH₃CN: 0.1 % formic acid in H₂0) to obtain 7-chloro-6-methyl-4-(methylsulfonyl)-3,4-dihydronaphthalen-l (2H)-one (430.0 mg, 58 %) as a white amorphous.

LC/MS ESI (+): 273 (M+l)

1H-NMR (400MHz, CDC1₃): δ 8.12 (s, 1H), 7.40 (s, 1H), 4.31-4.33 (m, 1H), 3.16- 3.26 (m, 1H), 2.93 (s, 3H), 2.86-2.91 (m, 1H), 2.64-2.71 (m, 1H), 2.51 -2.60 (m, 1H), 2.47 (s, 3H) (b) Synthesis of 7-chloro-l,l-difluoro-6-methyl-4-(methylsulfonyl)-l,2,3,4- tetrahydronaphthalene

A suspension of 7-chloro-6-methyl-4-(methylsulfonyl)-3,4-dihydronaphthalen- l(2H)-one (430.0 mg, 1.58 mmol) in DAST (2.0 ml, 15.14 mmol) was stirred at 65°C overnight. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 7-chloro- l,l-difluoro-6-methyl-4-(methylsulfonyl)-l,2,3,4-tetrahydronaphthalene (186.0 mg, 40 %) as a white amorphous.

LC/MS ESI (-): 293 (M-l)

1H-NMR (400MHz, CDC1₃): δ 7.77 (s, 1H), 7.60 (s, 1H), 4.22-4.26 (m, 1H), 2.77

(s, 3H), 2.65-2.73 (m, 1H), 2.51-2.62 (m, 2H), 2.43 (s, 3H), 2.23-2.35 (m, 1H)

(c) Synthesis of 6-(bromomethyl)-7-chloro-l,l-difluoro-4-(methylsulfonyl)- 1 ,2,3 ,4-tetrahydronaphthalene

To a solution of 7-chloro-l,l-difluoro-6-methyl-4-(methylsulfonyl)-l,2,3,4- tetrahydronaphthalene (150.0 mg, 0.51 mmol) and N-bromosuccinimide (109.0 mg, 0.61 mmol) in 1 ,2-dichloroethane (2545.0 μΐ) was added AIBN (8.4 mg, 0.05 mmol). The reaction mixture was stirred at 80°C for 1 hour. The reaction mixture was purified by flash column chromatography (silica gel, n-Hex : EtOAc = 5 : 1) to obtain 6-(bromomethyl)-7- chloro-l,l-difluoro-4-(methylsulfonyl)-l,2,3,4-tetrahydronaphthalene (105.0 mg, 55 %) as a white amorphous.

LC/MS ESI (-): 371 (M-l)

1H-NMR (400MHz, CDC1₃): δ 7.85 (s, 2H), 4.55-4.64 (m, 2H), 4.25-4.29 (m, 1H), 2.82 (s, 3H), 2.68-2.73 (m, 1H), 2.56-2.59 (m, 2H), 2.27-2.34 (m, 1H)

(d) Synthesis of 3-chloro-5,5-difluoro-8-(methylsulfonyl)-5, 6,7,8- tetrahydronaphthalene-2-carbaldehyde

A suspension of 6-(bromomethyl)-7-chloro-l,l-difluoro-4-(methylsulfonyl)- 1,2,3,4-tetrahydronaphthalene (105.0 mg, 0.28 mmol), N-methyl morpholine-N-oxide (49.4 mg, 0.42 mmol) and molecular sieve (4A) in CH₃CN (2.8 ml) was stirred at 25°C for 1 hour. The reaction mixture was purified by reversed-phase column chromatography (CI S- silica gel, 0.1% formic acid in CH₃CN: 0.1 % formic acid in H₂0) to obtain 3-chloro-5,5- difluoro-8-(methylsulfonyl)-5,6,7,8-tetrahydronaphthalene-2-carbaldehyde (60.0 mg, 69 %) as a light brown amorphous.

LC/MS ESI (-): 307 (M-l)

1H-NMR (400MHz, CDC1₃): δ 10.49 (s, 1H), 8.15 (s, 1H), 7.92 (s, 1H), 4.30-4.33 (m, 1H), 2.92 (s, 3H), 2.83-2.87 (m, 1H), 2.68-2.75 (m, 1H), 2.47-2.57 (m, 1H), 2.34-2.39 (m, 1H) (e) Synthesis of 8,8-difluoro-5-(methylsulfonyl)-5,6,7,8-tetrahydronaphtho[2,3- b]thiophene-2-carboxylic acid

To a solution of 3-chloro-5,5-difluoro-8-(methylsulfonyl)-5, 6,7,8- tetrahydronaphthalene-2-carbaldehyde (58.0 mg, 0.19 mmol) and Cs₂C0₃ (122.0 mg, 0.38 mmol) in DMF (939.0 μΐ) was added methyl thioglycoUate (18.5 μΐ, 0.21 mmol). The reaction mixture was stirred at 80°C for 1 hour. The reaction mixture was cooled to 60°C and LiOHH₂0 (79.0 mg, 1.88 mmol) was added and stirred for 1 hour. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 8,8-difluoro-5- (methylsulfonyl)-5,6,7,8-tetrahydronaphtho[2,3-b]thiophene-2-carboxylic acid (33.0 mg, 51 %) as an off-white amorphous.

LC/MS ESI (-): 345 (M-l)

1H-NMR (400MHz, DMS-</6): δ 8.48 (s, 1H), 8.18 (s, 1H), 8.13 (s, 1H), 4.89-4.90 (m, 1H), 3.17 (s, 3H), 2.66-2.73 (m, 2H), 2.32-2.42 (m, 2H) Intermediate 15) Synthesis of 4-(lH-pyrazol-l-yl)-3,4-dihydro-2_JH-thieno[3,2- g]chromene-7-carboxylic acid

(a) Synthesis of 3-(3-chloro-4-methylphenoxy)propanoic acid

To a solution of 2-chloro-4-(3,3-diethoxypropoxy)-l-methylbenzene (3.0 g, 11.00 mmol) in THF (27.5 ml)/H₂0 (27.5 ml) was added Oxone (10.1 g, 33.00 mmol). The reaction mixture was stirred at 25°C overnight and filtered. The filtrate was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, filtered and concentrated to give 3-(3-chloro-4-methylphenoxy)propanoic acid (2.4 g, 100 %) as a white amorphous.

LC/MS ESI (-): 213 (M-l)

Ή-NMR (400MHz, CDC1₃): δ 7.11 (d, 1H, J=8.4Hz), 6.92 (d, 1H, J=2.5Hz), 6.73 (dd, 1H, J=8.4, 2.4Hz), 4.21 (t, 2H, J=6.2Hz), 2.84 (t, 2H, J=6.2Hz), 2.29 (s, 3H)

(b) Synthesis of 7-chloro-6-methylchroman-4-one

3-(3-chloro-4-methylphenoxy)propanoic acid (2.4 g, 10.95 mmol) was dissolved in trifluoromethansulfonic acid (4.8 ml, 54.70 mmol) and the reaction mixture was stirred for 2.5 hours. Ice chip was added slowly and the resulting mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, filtered and concentrated. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 7-chloro- 6-methylchroman-4-one (795.0 mg, 37 %) as a white amorphous.

LC/MS ESI (+): 197 (M+l)

1H-NMR (400MHz, CDC1₃): δ 7.74 (s, 1H), 7.01 (s, 1H), 4.52 (t, 2H, J=6.5Hz), 2.84 (t, 2H, J=6.5Hz), 2.32 (s, 3H) (c) Synthesis of 6-(bromomethyl)-7-chlorochroman-4-one

The synthesis procedure of Intermediate 8-c was repeated except for using 7- chloro-6-methylchroman-4-one (950.0 mg, 4.83 mmol) to obtain 6-(bromomethyl)-7- chlorochroman-4-one (1.4 g, 87 % ) as an ivory solid.

LC/MS ESI (+): 275 (M+l)

1H-NMR (400MHz, CDC1₃): δ 7.97 (s, 1H), 7.10 (s, 1H), 4.54-4.58 (m, 4H), 2.82 (t, 2H, J=6.5Hz)

(d) Synthesis of 7-chloro-4-oxochromane-6-carbaldehyde

The synthesis procedure of Intermediate 8-d was repeated except for using 6-

(bromomethyl)-7-chlorochroman-4-one (1.3 g, 4.72 mmol) to obtain 7-chloro-4- oxochromane-6-carbaldehyde (660.0 mg, 66%) as a white solid.

LC/MS ESI (+): 211 (M+l)

1H-NMR (400MHz, CDC1₃): δ 10.34 (s, 1H), 8.50 (s, 1H), 7.10 (s, 1H), 4.64 (t, 2H, J=6.5Hz), 2.87 (t, 2H, J=6.5Hz)

(e) Synthesis of methyl 4-oxo-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylate

The synthesis procedure of Intermediate 8-d was repeated except for using 7- chloro-4-oxochromane-6-carbaldehyde (660.0 mg, 3.13 mmol) to obtain 4-oxo-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (580.0 mg, 71%) as an off white solid.

LC/MS ESI (+): 263 (M+l)

1H-NMR (400MHz, CDC1₃): δ 8.49 (s, 1H), 8.26 (s, 1H), 7.74 (s, 1H), 4.59 (t, 2H, J=6.4Hz), 3.87 (s, 3H), 2.87 (t, 2H, J=6.4Hz)

(f) Synthesis of methyl 4-hydroxy-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylate

To a solution of methyl 4-oxo-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylate (810.0 mg, 3.09 mmol) in EtOH (15.0 ml) was added NaBH₄ (140.0 mg, 3.71 mmol). The reaction mixture was stirred at 26°C for 2hours. H₂0 was added and insoluble white solid was filtered to give methyl 4-hydroxy-3,4-dihydro-2H-thieno[3,2-g]chromene- 7-carboxylate (665.0 mg, 81%) as a white solid.

LC/MS ESI (+): 265 (M+l)

1H-NMR (400MHz, DMSO-i¾: δ 8.11 (s, 1H), 7.79 (s, 1H), 7.40 (s, 1H), 5.55 (m, 1H), 4.75-4.79 (m, 1H), 4.23-4.33 (m, 2H), 3.85 (s, 3H), 2.02-2.09 (m, 1H), 1.88-1.94 (m, 1H)

(g) Synthesis of methyl 4-chloro-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylate

To a solution of methyl 4-hydroxy-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylate (200.0 mg, 0.76 mmol) in toluene (3784.0 μΐ) was added SOCl₂ (1 10.0 μΐ, 1.51 mmol). The reaction mixture was stirred at 60°C for 2 hours. After cooling, the reaction mixture was concentrated to give methyl 4-chloro-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxylate (210.0 mg, 98%) as a white amorphous without purification.

Ή-NMR (400MHz, DMSO-J₆): δ 8.13 (s, 1H), 8.02 (s, 1H), 7.51 (s, 1H), 5.72 (t, lH, J=3.4Hz), 4.36-4.46 (m, 2H), 3.86 (s, 3H), 2.42-2.48 (m, 1H), 2.26-2.31 (m, 1H)

(h) Synthesis of methyl 4-(lH-pyrazol-l-yl)-3,4-dmydro-2H-thieno[3,2- g] chromene-7-carboxylate

A solution of methyl 4-chloro-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylate (80.0 mg, 0.28 mmol), pyrazole (57.8 mg, 0.85 mmol) and K₂C0₃ (117.0 mg, 0.85 mmol) in DMA (2829.0 μΐ) was stirred at 60°C for Ihour and then stirred at 80°C. After Ihour, the reaction mixture was stirred at 100°C for Ihour. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in C¾CN: 0.1% formic acid in H₂0) to obtain methyl 4-(lH-pyrazol-l-yl)-3,4-dihydro-2H- thieno[3,2-g]chromene-7-carboxylate (26.0 mg, 29 %) as a white amorphous.

LC/MS ESI (+): 315 (M+1)

1H-NMR (400MHz, CDC13): δ 7.87 (s, 1H), 7.61 (d, 1H, J=1.5Hz), 7.51 (s, 1H), 7.37 (s, 1H), 7.22 (d, 1H, J=2.1Hz), 6.25-6.26 (m, 1H), 5.72 (t, 1H, J=5.1Hz), 4.31-4.37 (m, 1H), 4.15-4.21 (m, 1H), 3.92 (s, 3H), 2.56-2.64 (m, 1H), 2.40-2.48 (m, 1H)

(i) Synthesis of 4-(lH-pyrazol-l-yl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylic acid

To a suspension of methyl 4-(lH-pyrazol-l-yl)-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxylate (25.0 mg, 0.08 mmol) in THF (530.0 μ1)/Η₂0 (265.0 μΐ) was added LiOHH₂0 (33.4 mg, 0.78 mmol). The reaction mixture was stirred at 60°C for Ihour.

The reaction mixture was cooled to room temperature. 1N-HC1 was added and insoluble white solid was filtered to give 4-(l /-pyrazol-l-yl)-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxylic acid (17.0 mg, 71%) as a white solid.

LC/MS ESI (-): 299 (M-l)

Intermediate 16) Synthesis of 4-(2-oxopyrrolidin-l-yl)-3,4-dihydro-2H- thieno [3,2-g] chromene-7-carboxylic acid To a solution of 2-Pyrrolidinone (64.5 μΐ, 0.85 mmol) in DMA (4.2 ml) was added

NaH (33.9 mg, 0.85 mmol). The reaction mixture was stirred at 100°C for 30 minutes, methyl 4-chloro-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (120.0 mg, 0.42 mmol) was added and stirred for Ihour. The reaction mixture was cooled to 80°C. LiOHH₂0 (53.4 mg, 1.27 mmol) was added and stirred for Ihour. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, 0.1 % formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain 4-(2-oxopyrrolidin-l-yl)-3,4-dihydro-2H- thieno[3,2-g]chromene-7-carboxylic acid (13.0 mg, 10 %) as a white amorphous.

LC/MS ESI (+): 318 (M+l)

Ή-NMR (400MHz, DMS-i 6): 6 7.93 (s, 1H), 7.57 (s, 1H), 7.42 (s, 1H), 5.41-5.45 (m, 1H), 4.27-4.40 (m, 2H), 3.23-3.32 (m, 1H), 2.95-3.01 (m, 1H), 2.36-2.43 (m, 2H), 2.10-2.19 (m, 1H), 1.90-2.01 (m, 3H)

Intermediate 17) Synthesis of 4-cyano-3,4-dihydro-2 T-thieno[3,2-^]chromene- 7-carboxyIic acid

(a) Synthesis of methyl 4-bromo-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylate

Methyl 4-hydroxy-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (210.0 mg, 0.79 mmol) was dissolved in CH₂C1₂ (3.9 mL), and PBr₃ (323.0 mg, 1.19 mmol) was added. After stirring at room temperature for 2 hours, and the reaction mixture was concentrated under a reduced pressure to obtain crude solid compound of methyl 4-bromo- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (250.0 mg. 95%).

Ή-NMR (400MHz, DMSO-d₆): δ 8.18 (s, 1H), 8.07 (s, 1H), 7.54 (s, 1H), 6.05- 6.06 (m, 1H), 4.54-4.57 (m, 2H), 3.92 (s, 3H), 2.39-2.45 (m, 2H)

(b) Synthesis of methyl 4-cyano-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylate

Methyl 4-bromo-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (300.0 mg, 0.91 mmol) was dissolved in DMA (9.1 mL), and NaCN (90.0 mg, 1.83 mmol) was added at room temperature. The mixture was stirred at 60°C for 2 hours, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, filtered and concentrated under a reduced pressure. The residue was purified by reversed- phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain methyl 4-cyano-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylate (39.0 mg, 15%) as an off-white solid.

LC/MS ESI (+): 274 (M+l)

Ή-NMR (400MHz, DMSO-<¾: δ 8.17 (s, 1H), 8.03 (s, 1H), 7.56 (s, 1H), 4.68 (t, 1H, J=6.0Hz), 4.31 (t, 2H, J=5.2Hz), 3.87 (s, 3H), 2.29-2.39 (m, 2H) (c) Synthesis of 4-cyano-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylic acid

Methyl 4-cyano-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (39.0 mg, 0.14 mmol) was dissolved in THF/¾0 (1.5 mL, 3/1 v/v), and LiOH H₂0 (17.9 mg, 0.43 mmol) was added. After stirring at room temperature for 15 hours, the reaction mixture was concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN : 0.1 % formic acid in H₂0) to obtain methyl 4-cyano-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (17.0 mg, 46%) as a white solid.

LC/MS ESI (+): 260 (M+l)

1H-NMR (400MHz, DMSO-d₆): δ 13.3 (br, 1H), 7.96 (s, 1H), 7.91 (s, 1H), 7.44 (s, 1H), 4.60 (t, 1H, J=6.0Hz), 4.22 (t, 2H, J=5.2 Hz), 2.20-2.26 (m, 2H)

Intermediate 18) Synthesis of 4-azido-3,4-dmydro-2H-thieno[3,2-g]chromene- 7-carboxylic acid (a) Synthesis of methyl 4-azido-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylate

Methyl 4-bromo-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (95.0 mg, 0.29 mmol) was dissolved in DMA (2.9 mL), The mixture was stirred at 60°C for 3 hours, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, and concentrated under a reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = 6 : 1) to obtain methyl 4- azido-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (50.0 mg, 60%) as a white solid.

LC/MS ESI (+): 290 (M+l)

Ή-NMR (400MHz, CDC1₃): δ 7.97 (s, 1H), 7.72 (s, 1H), 7.32 (s, 1H), 4.77 (t, 1H,

J=4.1 Hz), 4.31-4.35 (m, 2H), 3.93 (s, 3H), 2.10-2.23 (m, 2H)

(b) Synthesis of 4-azido-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylic acid Methyl 4-azido-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxylate (15.0 mg, 0.05 mmol) was dissolved in THF/H₂0 (0.5 mL, 3/1 v/v), and LiOH H₂0 (21.7 mg, 0.52 mmol) was added. The reaction mixture was stirred at 60°C for 15 hours, IN HCl (3.0 mL) was added, and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure to obtain 4-azido-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxylic acid (10.0 mg, 70%) as an off-white solid.

LC/MS ESI (-): 274 (M-l)

Example 1) Synthesis of 7V-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-l- (methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-6][l,4]oxazine-7- carboxamide

(a) Synthesis of 2-chloro-6-(4-chlorophenoxy)pyridin-4-amine

2,6-Dichloropyridine-4-amine (3.0 g, 18.40 mmol) and 4-chlorophenol (4.7 g, 36.80 mmol) were dissolved in sulfolane (96.0 mL) and K₂C0₃ (5.1 g, 36.80 mmol) was added. The reaction mixture was stirred at 160°C for 24 hours, cooled to room temperature, H₂0 was added, and extracted with EtOAc. The organic extract was washed with IN NaOH aqueous solution and brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN : 0.1% formic acid in H₂0) to obtain 2-chloro-6-(4-chlorophenoxy)pyridin-4-amine (2.5 g, 53%) as a white solid.

LC/MS ESI (+): 255 (M+l)

1H-NMR (400MHz, DMSO- ₆): δ 7.45 (d, 2H, J=8.8Hz), 7.12 (d, 2H, J=8.8Hz), 6.55 (brs, 2H), 6.31 (d, lH, J=1.6Hz), 5.93 (d, lH, J=1.6Hz)

(b) Synthesis of N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-l- (methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxamide 1 -(Methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[ 1 ,2-b] [ 1 ,4]oxazine-7- carboxylic acid (190.0 mg, 0.61 mmol) was dissolved in CH₂C1₂ (6.1 mL), and DMF (1.2 μί, 0.01 mmol) and (COCl) (51.6 μί, 0.61 mmol) were added. The reaction mixture was stirred at 25 °C for 2 hours and concentrated under reduced pressure to obtain 1- (methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carbonyl chloride. To the residue, 2-chloro-6-(4-chlorophenoxy)pyridin-4-amine (155.0 mg, 0.61 mmol) and 1,4-Dioxane (2.0 mL) were added and the reaction mixture was stirred at 80°C for 15 hours and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN : 0.1% formic acid in H₂0) to obtain N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-l- (methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l ,2-b][l,4]oxazine-7-carboxamide (166.0 mg, 50%) as a white solid.

LC/MS ESI (+): 550 (M+l)

1H-NMR(400MHz, DMSO-^₆): δ 10.87 (brs, 1H), 8.20 (s, 1H), 8.10 (s, 1H), 7.58 (s, 2H), 7.45 (dd, 2H, J=8.8, 2.1Hz), 7.22 (s, 1H), 7.18 (dd, 2H, J=8.8, 2.1Hz), 4.29 (t, 2H, J=4.9Hz), 3.82 (t, 2H, J=4.6Hz), 3.11 (s, 3H)

Compounds from Examples 2 to 14 were synthesized through the synthesis route of Example 1, and data of these compounds are listed as follows.

[Table 2]

LC/MS ESI (+): 564 (M+l)

'H-NMR(400MHz, DMSO-< ₆): δ 11.10 (s,

N-(2-chloro-6-(4-chlorophenoxy)pyridin- IH), 8.36 (s, IH), 8.08 (s, IH), 7.90 (s, IH), 4-yl)-l -(methylsulfonyl)-l ,2,3,4- 7.70 (d, IH, J=1.2Hz), 7.55 (d, 2H, J=8.8Hz), tetrahydrothieno[3',2':4,5]benzo[l ,2- 7.33 (s, IH, J=1.2Hz), 7.29 (d, 2H, J=8.8Hz), b] [ 1 ,4]oxazepine-8-carboxamide

4.15-4.17 (m, 2H), 3.75-3.77 (m, 2H), 3.11 (s, 3H), 2.08-2.10 (m, 2H)

LC/MS ESI (+): 576 (M+l)

'H-NMR(400MHz, DMSO-i/₆): δ 10.98 (s,

N-(2-chloro-6-(4-chlorophenoxy)pyridin- IH), 8.30 (s, IH), 8.21 (s, IH), 8.14 (s, IH), 4-yl)-8,8-dimethyl-5-(methylsulfonyl)- 7.67 (s, IH), 7.53 (d, 2H, J=8.7Hz), 7.32 (s, 5 ,6,7,8 -tetrahydrothieno [2,3 -g] quinoline- IH), 7.26 (d, 2H, J=8.7Hz), 3.79 (t, 2H, 2-carboxamide

J=5.7Hz), 3.12 (s, 3H), 1.85 (t, 2H, J=5.7Hz), 1.37 (s, 6H)

LC/MS ESI (+): 679 (M+l)

tert-butyl 7-((2-chloro-6-(4- ^]H-NMR (400MHz, DMSO-rf₆): δ 10.93 (brs, chlorophenoxy)pyridin-4-yl)carbamoyl)- IH), 8.35 (s, IH), 8.24 (s, IH), 8.03 (d, IH, 1 -(methylsulfonyl)-2,3- J=8.7Hz), 7.60 (d, IH, J=1.3Hz), 7.47 (d, 2H, dihydrothieno[2,3 -g]quinoxaline-4( 1 H)- J=8.8Hz), 7.24 (d, IH, J=1.3 Hz), 7.19 (d, 2H, carboxylate J=8.8Hz), 3.82-3.84 (m, 2H), 3.79-3.80 (m,

2H), 3.05 (s, 3H), 1.43 (s, 9H)

LC/MS ESI (+): 548 (M+1)

Ή-NMR (400MHz, DMSO-<¾: δ 10.98 (s,

N-(2-chloro-6-(4-chlorophenoxy)pyridin- IH), 8.32 (s, IH), 8.13 (s, IH), 7.90 (s, IH), 4-yl)-5-(methylsulfonyl)-5,6,7,8- 7.66 (s, IH), 7.53 (d, 2H, J=8.8Hz), 7.28 (s, tetrahydrothieno[2,3-g]quinoline-2- IH), 7.26 (d, 2H, J=8.8Hz), 3.75 (t, 2H, carboxamide

J=6.3Hz ), 3.06 (s, 3H), 2.93 (t, 2H, J=6.5Hz), 1.95-1.99 (m, 2H)

LC/MS ESI (+): 563 (M+1)

Ή-NMR (400MHz, DMSO-rf₆): δ 10.81 (brs,

N-(2-chloro-6-(4-chlorophenoxy)pyridin- IH), 8.20 (s, IH), 7.89 (s, IH), 7.67 (s, IH), 4-yl)-4-methyl- 1 -(methylsulfonyl)- 7.55 (d, 2H, J=8.7Hz), 7.33 (s, 1Ή), 7.32 (s, 1 ,2,3,4-tetrahydrothieno[2,3- IH), 7.28 (d, 2H, J=8.7Hz), 3.79 (t, 2H, g]quinoxaline-7-carboxamide J=5.3Hz ), 3.53 (t, 2H, J=5.3Hz ), 3.04 (d, 6H,

J=2.6Hz)

LC/MS ESI (+): 549 (M+l).

Ή-NMR (400MHz, DMSO-^): δ 10.94 (s, IH), 8.31 (s, IH), 8.08 (s, IH), 7.67 (d, IH,

N-(2-chloro-6-(4-chlorophenoxy)pyridin- J=l . lHz), 7.59 (s, IH), 7.53 (d, 2H, J=8.9Hz), 4-yl)-4-(methylsulfonyl)-3,4-dihydro-2H- 7.31 (d, IH, J=l .lHz), 7.27 (d, 2H, J=8.9Hz), thieno [3 ,2-g] chromene-7-carboxamide

4.82 (m, IH), 4.46-4.53 (m, IH), 4.31-4.36 (m, IH), 3.18 (s, 3H), 2.53-2.69 (m, IH), 2.29-2.40 (m, IH). LC/MS ESI (+): 563 (M+l)

Ή- MR (400MHz, DMSO-d₆): δ 10.90 (s, IH), 8.28 (s, IH), 8.03 (s, IH), 7.69 (s, IH),

N-(2-chloro-6-(4-chlorophenoxy)pyridin- 7.59 (d, IH, J=1.4Hz), 7.44-7.48 (m, 2H), 4-yl)-5-(methylsulfonyl)-2,3,4,5- 7.25 (s, IH), 7.17-7.21 (m, 2H), 4.78 (t, IH, tetrahydrothieno[3',2':4,5]benzo[l,2- J=5.4Hz), 4.29-4.34 (m, IH), 3.68-3.73 (m, b] oxepine-8 -carboxamide

IH), 2.81 (s, 3H), 2.25-2.50 (m, IH), 2.16- 2.19 (m, IH), 2.04-2.04 (m, IH), 1.70-1.74 (m, IH)

LC/MS ESI (+): 547 (M+l)

Ή-NMR (400MHz, DMSO- ₆): δ 1 1.0 (s,

N-(2-chloro-6-(4-chlorophenoxy)pyridin- IH), 8.35 (s, IH), 8.12 (s, IH), 7.91 (s, IH), 4-yl)-5-(methylsulfonyl)-5,6,7,8- 7.66 (d, IH, J=1.3Hz), 7.53 (d, 2H, J=8.9Hz), tetrahydronaphtho [2,3 -0]thiophene-2- 7.31 (d, IH, J=1.3Hz), 7.26 (d, 2H, J=8.9Hz), carboxamide 4.76-4.79 (m, IH), 3.01 (s, 3H), 2.82-2.94 (m,

2H), 2.43-2.46 (m, IH), 2.14-2.20 (m, 2H), 1.62-1.66 (m, IH)

LC/MS ESI (+): 563 (M+l)

Ή-NMR (400MHz, DMSO-d₆): δ 10.94 (brs,

N-(2-chloro-6-(4-chlorophenoxy)pyridin- IH), 8.29 (s, IH), 8.25 (s, IH), 7.65 (s, IH), 4-yl)-4-methyl-4-(methylsulfonyl)-3,4- 7.57 (s, IH), 7.52 (d, 2H, J=8.5Hz), 7.30 (s, dihydro-2H-thieno[3,2-g]chromene-7- IH), 7.25 (d, 2H, J=8.3Hz), 4.49-4.54 (m, carboxamide

IH), 4.20-4.24 (m, IH), 2.98 (s, 3H), 2.62 (m, IH), 2.15-2.22 (m, IH), 1.84 (s, 3H)

LC/MS ESI (+): 549 (M+l)

'H-NMR(400MHz, DMSO- ₆):8 10.96 (brs, IH), 8.32 (s, IH), 8.09 (s, IH), 7.87 (s, IH),

N-(2-chloro-6-(4-chlorophenoxy)pyridin- 7.60 (d, IH, J=1.2Hz), 7.45-7.47 (m, 2H), 4-yl)-5-(methylsulfonyl)-5,8-dihydro-6H- 7.25 (d, IH, J=0.8Hz), 7.18-7.22 (m, 2H), thieno[3,2-g]isochromene-2-carboxaraide 4.96 (d, IH, J=15.6Hz), 4.82 (d, IH,

J=15.6Hz), 4.60 (d, IH, J=12.8Hz), 4.48 (s, IH), 4.03 (dd, IH, J=12.8, 3.6Hz), 2.63 (s, 3H)

LC/MS ESI (+): 567 (M+l)

Ή-NMR (400MHz, DMSO- ₆):

N-(2-chloro-6-(4-chlorophenoxy)pyridin-

8 10.92 (brs, IH), 8.27 (s, IH), 8.24 (s, IH), 4-yl)-4-fluoro-4-(methylsulfonyl)-3,4-

7.64 (s, IH), 7.58 (s, IH), 7.46 (d, 2H, dihydro-2H-thieno[3,2-g]chromene-7-

J=8.8Hz), 7.21 (s, IH), 7.18 (d, 2H, J=8.8Hz), carboxamide

4.46-4.52 (m, IH), 4.33-4.38 (m, IH), 2.93 (s,

3H), 2.87-2.93 (m, IH), 2.60-2.67 (m, IH)

N-(2-chloro-6-(4-chlorophenoxy)pyridin- LC/MS ESI (-): 581 (M-l)

4-yl)-8,8-difluoro-5-(methylsulfonyl)- Ή-NMR (400MHz, DMSO-i/6): δ 1 1.13 (s, 5,6,7,8-tetrahydronaphtho[2,3- IH), 8.57 (s, IH), 8.43 (s, IH), 8.23 (s, IH), Z>]thiophene-2 -carboxamide 7.67 (d, IH, J=l . lHz), 7.52 (d, 2H, J=8.9Hz), 7.31 (d, 1H, J=0.7Hz), 7.26 (d, 2H, J=8.9Hz),

4.91 (m, 1H), 3.19 (s, 3H), 2.80-2.90 (m, 1H), 2.70-2.75 (m, 1H), 2.36-2.45 (m, 2H)

Example 15) Synthesis of V-(2-chloro-6-(p-tolyloxy)pyridin-4-yl)-4- (methylsulfonyl)-3,4-dmydro-2H-thieno[3,2-^]chromene-7-carboxamide

(a) Synthesis of 2-chloro-6-(p-tolyloxy)pyridin-4-amine

To a solution of 2,6-dichloropyridin-4-amine (200.0 mg, 1.23 mmol) in sulfolane (4090.0 μΐ) were added -cresol (265.0 mg, 2.45 mmol) and K₂C0₃ (339.0 mg, 2.45 mmol). The reaction mixture was stirred at 160°C for 30 hours. The reaction mixture was extracted with EtOAc. The organic extract was washed with IN-NaOH and brine, dried over anhydrous Na₂S0₄, filtered and concentrated. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1 % formic acid in H₂0) and solidification with ACN/ether/Hex to obtain 2-chloro-6-(p-tolyloxy)pyridin-4- amine (160.0 mg, 56 %) as a light brown amorphous.

1H-NMR (400MHz, CDC1₃): δ 7.17 (d, 2H, J=8.2Hz), 7.00 (d, 2H, J=8.5Hz), 6.30

(d, 1H, J=1.7Hz), 5.81 (d, 1H, J=1.7Hz), 4.22 (brs, 2H), 2.35 (s, 3H)

(b) Synthesis of N-(2-chloro-6-(p-tolyloxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxamide

The synthesis procedure of Example 1-b was repeated except for using 2-chloro-6-

(p-tolyloxy)pyridin-4-amine (41.3 mg, 0.18 mmol) to obtain V-(2-chloro-6-(p- tolyloxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxamide (51.0 mg, 60 %) as a white amorphous.

LC/MS ESI (+): 529 (M+l)

Ή-NMR (400MHz, DMSO- 6): δ 10.89 (s, 1H), 8.29 (s, 1H), 8.05 (s, lH), 7.64 (d,

1H, J=1.4Hz), 7.58 (s, 1H), 7.27 (d, 2H, J=8.3Hz), 7.21 (d, 1H, J=1.4Hz), 7.08 (d, 2H, J=8.4Hz), 4.81 (m, 1H), 4.44-4.51 (m, 1H), 4.29-4.34 (m, 1H), 3.16 (s, 3H), 2.59-2.67 (m, 1H), 2.32-2.34 (m, 4H) Compounds from Examples 16 to 42 were synthesized through the synthesis route of Example 15, and data of these compounds are listed as follows.

[Table 3]

Ex. Compound Analysis data LC/MS ESI (+): 583 (M+l)

Ή-NMR (400MHz, DMSO-<¾: δ 10.97 (s,

N-(2-chloro-6-(3- IH), 8.32 (s, IH), 8.08 (s, IH), 7.65-7.74 (m,

(trifluoromethyl)phenoxy)pyridin-4-yl)- 4H), 7.55-7.59 (m, 2H), 7.40 (d, IH, 4-(methylsulfonyl)-3,4-dihydro-2H- 7=1.2Hz), 4.81-4.84 (m, IH), 4.49-4.53 (m, thieno [3 ,2-g] chromene-7 -carboxamide

IH), 4.31-4.34 (m, IH), 3.17 (s, 3H), 2.60- 2.68 (m, IH), 2.35-2.36 (m, IH)

LC/MS ESI (+): 583 (M+l)

Ή-NMR (400MHz, DMSO-i/₆): δ 10.98 (s,

N-(2-chloro-6-(4- IH), 8.32 (s, IH), 8.08 (s, IH), 7.85 (d, 2H,

(trifluoromethyl)phenoxy)pyridin-4-yl)- J=8.6Hz), 7.71 (s, IH), 7.59 (s, IH), 7.41-7.45 4-(methylsulfonyl)-3,4-dihydro-2H- (m, 3H), 4.82-4.83 (m, IH), 4.49-4.52 (m, thieno [3 ,2-g] chromene-7 -carboxamide

IH), 4.31-4.35 (m, IH), 3.17 (s, 3H), 2.60- 2.65 (m, IH), 2.36-2.39 (m, IH)

LC/MS ESI (+): 583 (M+l)

Ή-NMR (400MHz, DMSO-<¾: δ 10.98 (s,

N-(2-chloro-6-(3,5- IH), 8.32 (s, IH), 8.08 (s, IH), 7.70 (d, 2H, dichlorophenoxy)pyridin-4-yl)-4- J=1.2Hz), 7.59 (s, IH), 7.56 (t, IH, J=2.0Hz), (methylsulfonyl)-3,4-dihydro-2H- 7.44 (d, 2H, J=2.0Hz), 7.37 (s, IH), 4.82-4.84 thieno [3 ,2-g] chromene-7 -carboxamide (m, IH), 4.46-4.52 (m, IH), 4.32-4.35 (m,

IH), 3.17 (s, 3H), 2.60-2.65 (m, IH), 2.36- 2.39 (m, IH)

LC/MS ESI (+): 567 (M+l)

¾-NMR (400MHz, DMSO-i/₆): δ 10.96 (s,

N-(2-chloro-6-(4-chloro-3- IH), 8.30 (s, IH), 8.07 (s, IH), 7.67-7.69 (m, fluorophenoxy)pyridin-4-yl)-4- 2H), 7.58 (s, IH), 7.47 (d, IH, J=10.3Hz), (methylsulfonyl)-3,4-dihydro-2H- 7.35 (s, IH), 7.15 (d, IH, J=10.3Hz), 4.83- thieno[3,2-g]chromene-7-carboxamide 4.85 (m, IH), 4.49-4.52 (m, IH), 4.30-4.34

(m, IH), 3.17 (s, 3H), 2.60-2.65 (m, IH), 2.36- 2.39 (m, IH)

LC/MS ESI (+): 563 (M+l)

]H-NMR (400MHz, DMSO-i/₆): 10.92 (brs,

N-(2-chloro-6-(4-chloro-3- IH), 8.29 (s, IH), 8.06 (s, IH), 7.67 (s, IH), methylphenoxy)pyridin-4-yl)-4- 7.58 (s, IH), 7.50 (d, IH, J=8.7Hz), 7.25-7.26 (methylsulfonyl)-3,4-dihydro-2H- (m, 2H), 7.09 (dd, IH, J=8.6, 2.8Hz), 4.82 (m, thieno[3,2-g]chromene-7-carboxamide IH), 4.45-4.51 (m, IH), 4.30-4.34 (m, IH),

3.16 (s, 3H), 2.60-2.64 (m, IH), 2.32-2.35 (m, 4H)

LC/MS ESI (+): 563 (M+l)

N-(2-chloro-6-(4-chloro-2- Ή-NMR (400MHz, OMSO-d₆): 10.92 (s, IH), methylphenoxy)pyridin-4-yl)-4- 8.30 (s, IH), 8.06 (s, IH), 7.64 (m, IH), 7.58 (methylsulfonyl)-3,4-dihydro-2H- (s, IH), 7.47 (m, IH), 7.35 (dd, IH, J=8.6, thieno [3 ,2-g] chromene-7 -carboxamide 2.6Hz), 7.26 (m, IH), 7.19 (d, IH, J=8.6Hz),

4.81 (m, IH), 4.45-4.51 (m, IH), 4.30-4.34 (m, 1Η), 3.19 (s, 3Η), 2.60-2.67 (m, 1Η), 2.32- 2.36 (m, lH), 2.12 (s, 3H)

LC/MS ESI (+): 545 (M+l)

Ή-NMR (400MHz, DMSO-i/6): δ 10.88 (s,

N-(2-chloro-6-(4- IH), 8.29 (s, IH), 8.05 (s, IH), 7.63 (d, IH, methoxyphenoxy)pyridin-4-yl)-4- J=1.2Hz), 7.58 (s, IH), 7.19 (d, IH, J=1.2Hz), (methylsulfonyl)-3,4-dihydro-2H- 7.14 (d, 2H, J=9.0Hz), 7.01 (d, 2H, 7=9.0Hz), thieno[3,2-g]chromene-7-carboxamide 4.81 (m, IH), 4.44-4.51 (m, IH), 4.29-4.34

(m, IH), 3.79 (s, 3H), 3.16 (s, 3H), 2.59-2.67 (m, IH), 2.30-2.39 (m, IH)

LC/MS ESI (+): 567 (M+l)

Ή-NMR (400MHz, DMSO-i¾: δ 10.90 (brs,

N-(2-chloro-6-(4-chloro-2- IH), 8.24 (s, IH), 8.00 (s, IH), 7.62 (dd, IH, fluorophenoxy)pyridin-4-yl)-4- J=10.4, 2.4Hz), 7.57 (s, IH), 7.51 (s, IH), (methylsulfonyl)-3,4-dihydro-2H- 7.30-7.41 (m, 3H), 4.73-4.75 (m, IH), 4.38- thieno[3,2-g]chromene-7-carboxamide

4.45 (m, IH), 4.23-4.27 (m, IH), 3.09 (s, 3H), 2.53-2.61 (m, IH), 2.27-2.32 (m, IH)

LC/MS ESI (+): 585 (M+l)

Ή-NMR (400MHz, DMSO- ₆): δ 10.88 (brs,

N-(2-chloro-6-(3,4- IH), 8.23 (s, IH), 8.00 (s, IH), 7.65 (d, IH, dichlorophenoxy)pyridin-4-yl)-4- 7=8.8Hz), 7.60 (s, IH), 7.57 (d, IH, J=2.7Hz), (methylsulfonyl)-3,4-dihydro-2H- 7.51 (s, IH), 7.27 (s, IH), 7.20 (dd, IH, 7=8.8, thieno[3,2-g]chromene-7-carboxamide 2.7Hz), 4.73-4.75 (m, IH), 4.38-4.45 (m, IH),

4.23-4.27 (m, IH), 3.09 (s, 3H), 2.53-2.60 (m, IH), 2.27-2.32 (m, IH)

LC/MS ESI (+): 549 (M+l)

Ή-NMR (400MHz, OMSO-d₆): δ 10.87 (brs, IH), 8.24 (s, IH), 8.00 (s, IH), 7.60 (s, IH),

N-(2-chloro-6-(3-chlorophenoxy)pyridin- 7.51 (s, IH), 7.43 (t, IH, 7=8. lHz), 7.28-7.31 4-yl)-4-(methylsulfonyl)-3,4-dihydro-2H- (m, 2H), 7.26 (d, IH, 7=1.3Hz), 7.12-7.15 (m, thieno[3,2-g]chromene-7-carboxamide

IH), 4.73-4.75 (m, IH), 4.38-4.45 (m, IH), 4.23-4.27 (m, IH), 3.09 (s, 3H), 2.53-2.60 (m, IH), 2.27-2.32 (m, IH)

LC/MS ESI (+): 533 (M+l)

Ή-NMR (400MHz, DMSO-i¾: δ 10.84 (brs,

N-(2-chloro-6-(4-fluorophenoxy)pyridin- IH), 8.23 (s, IH), 7.99 (s, IH), 7.57 (s, IH), 4-yl)-4-(methylsulfonyl)-3,4-dihydro-2H- 7.51 (s, IH), 7.18-7.26 (m, 5H), 4.74-4.76 thieno [3 ,2-g] chromene-7-carboxamide (m, IH), 4.38-4.44 (m, IH), 4.24-4.26 (m,

IH), 3.09 (s, 3H), 2.53-2.60 (m, IH), 2.27- 2.32 (m, IH)

N-(2-chloro-6-(3-chloro-4- LC/MS ESI (+): 567 (M+l)

fluorophenoxy)pyridin-4-yl)-4- ¹H-NMR(400MHz, DMSO-i/₆): δ 10.87 (brs,

(methylsulfonyl)-3,4-dihydro-2H- IH), 8.23 (s, IH), 8.00 (s, IH), 7.59 (d, IH, thieno [3 ,2-g] chromene-7-carboxamide J=0.8Hz), 7.44-7.53 (m, 3H), 7.19-7.24 (m,

2H), 4.74-4.75 (m, IH), 4.38-4.45 (m, IH), 4.23-4.27 (m, IH), 3.09 (s, 3H), 2.53-2.57 (m, IH), 2.24-2.32 (m, IH)

LC/MS ESI (+): 599 (M+l)

]H-NMR(400MHz, DMSO- ₆): δ 10.95 (brs,

N-(2-chloro-6-(4- IH), 8.28 (brs, IH), 8.06 (s, IH), 7.66 (s, IH),

(trifluoromethoxy)phenoxy)pyridin-4-yl)- 7.57 (s, IH), 7.47 (d, 2H, J=8.8Hz), 7.33-7.36

4-(methylsulfonyl)-3,4-dihydro-2H- (m, 3H), 4.81-4.82 (m, IH), 4.45-4.52 (m, thieno[3,2-g]chromene-7-carboxamide

IH), 4.29-4.34 (m, IH); 3.16 (s, 3H), 2.60- 2.64 (m, IH), 2.31-2.39 (m, IH)

LC/MS ESI (+): 599 (M+l)

'H-NMR^OOMHz, DMSCWe): δ 10.96 (brs,

N-(2-chloro-6-(3- IH), 8.30 (s, IH), 8.07 (s, IH), 7.67 (d, IH,

(trifluoromethoxy)phenoxy)pyridin-4-yl)- 7=1.2Hz), 7.58-7.62 (m, 2H), 7.37 (d, IH,

4-(methylsulfonyl)-3,4-dihydro-2H- J=0.8Hz), 7.26-7.32 (m, 3H), 4.81-4.82 (m, thieno[3,2-g]chromene-7-carboxamide IH), 4.45-4.52 (m, IH), 4.30-4.34 (m, IH),

3.16 (s, 3H), 2.60-2.64 (m, IH), 2.30-2.39 (m, IH)

LC/MS ESI (+): 580 (M+l)

¾-NMR (400MHz, OMSO-d₆): δ 10.87 (brs,

N-(2-chloro-6-(3-chloro-5- IH), 8.23 (s, IH), 7.99 (s, IH), 7.61 (s, IH), methoxyphenoxy)pyridin-4-yl)-4- 7.50 (s, IH), 7.22 (s, IH), 6.89-6.90 (m, IH), (methylsulfonyl)-3,4-dihydro-2H- 6.85-6.86 (m, IH), 6.74-6.75 (m, IH), 4.74- thieno[3,2-g]chromene-7-carboxamide 4.75 (m, IH), 4.38-4.43 (m, IH), 4.23-4.27

(m, IH), 3.75 (s, 3H), 3.09 (s, 3H), 2.53-2.57 (m, IH), 2.29-2.32 (m, IH)

LC/MS ESI (+): 566 (M+l)

Ή-NMR (400MHz, OMSO-d₆): δ 10.90 (brs,

N-(2-chloro-6-(3-chloro-5- IH), 8.23 (s, IH), 8.00 (s, IH), 7.62 (s, IH), fluorophenoxy)pyridin-4-yl)-4- 7.51 (s, IH), 7.31-7.32 (m, IH), 7.30 (s, IH), (methylsulfonyl)-3,4-dihydro-2H- 7.20 (s, IH), 7.16-7.19 (m, IH), 4.74-4.75 (m, thieno [3 ,2-g] chromene-7-carboxamide IH), 4.38-4.43 (m, IH), 4.23-4.27 (m, IH),

3.09 (s, 3H), 2.53-2.57 (m, IH), 2.29-2.32 (m, IH)

LC/MS ESI (+): 563 (M+l)

Ή-NMR (400MHz, DMSO-i/₆): δ 10.86 (brs,

N-(2-chloro-6-(3-fluoro-5- IH), 8.22 (s, IH), 7.99 (s, IH), 7.61 (s, IH), methoxyphenoxy)pyridin-4-yl)-4- 7.50 (s, IH), 7.22 (s, IH), 6.64-6.72 (m, 2H), (methylsulfonyl)-3,4-dibydro-2H- 6.61 (s, IH), 4.74-4.76 (m, IH), 4.38-4.44 (m, thieno [3 ,2-g] chromene-7-carboxamide

IH), 4.24-4.26 (m, IH), 3.72 (s, 3H), 3.09 (s, 3H), 2.53-2.60 (m, IH), 2.27-2.32 (m, IH) LC/MS ESI (+): 529 (M+l)

Ή-NMR (400MHZ, DMSO-<¾: δ 10.83 (brs, IH), 8.22 (s, IH), 7.98 (s, IH), 7.59 (s, IH),

N-(2-chloro-6-(m-tolyloxy)pyridin-4-yl)- 7.50 (s, IH), 7.28 (t, IH, J=7.7Hz), 7.15 (s, 4-(methylsulfonyl)-3,4-dihydro-2H- IH), 7.03 (d, IH, J=7.6Hz), 6.95 (s, IH), 6.91- thieno [3 ,2-g] chromene-7-carboxamide

6.93 (m, IH), 4.74-4.76 (m, IH), 4.38-4.44 (m, IH), 4.24-4.26 (m, IH), 3.09 (s, 3H), 2.53- 2.60 (m, IH), 2.27 (s, 3H), 2.27-2.32 (m, IH)

LC/MS ESI (+): 551 (M+l)

'H-NMR^OOMHz, DMSO-i/₆): δ 10.94 (brs,

N-(2-chloro-6-(3,4- IH), 8.29 (s, IH), 8.06 (s, IH), 7.65 (s, IH), difluorophenoxy)pyridin-4-yl)-4- 7.47-7.58 (m, 3H), 7.31 (s, IH), 7.10-7.14 (m, (methylsulfonyl)-3,4-dihydro-2H- IH), 4.81-4.82 (m, IH), 4.45-4.51 (m, IH), thieno [3 ,2-g] chromene-7-carboxamide

4.30-4.34 (m, IH), 3.16 (s, 3H), 2.60-2.64 (m, IH), 2.30-2.39 (m, IH)

LC/MS ESI (+): 567 (M+l)

Ή-NMR (400MHz, DMSO-i ₆): 10.99 (brs,

N-(2-chloro-6-(5-chloro-2- IH), 8.32 (s, IH), 8.08 (s, IH), 7.65 (s, IH), fluorophenoxy)pyridin-4-yl)-4- 7.62 (dd, IH, J=6.9, 2.6Hz), 7.59 (s, IH), (methylsulfonyl)-3,4-dihydro-2H- 7.47-7.52 (m, IH), 7.41-7.45 (m, 2H), 4.82 thieno[3,2-g]chromene-7 -carboxamide (m, IH), 4.45-4.49 (m, IH), 4.31-4.35 (m,

IH), 3.17 (s, 3H), 2.60-2.64 (m, IH), 2.35- 2.39 (m, IH)

LC/MS ESI (+): 567 (M+l)

Ή-NMR (400MHz, DMSO-c?₆): 11.00 (s, IH), 8.32 (s, IH), 8.09 (s, IH), 7.66 (d, IH,

N-(2-chloro-6-(3-chloro-2- 7=1.2Hz), 7.59 (s, IH), 7.55 (td, IH, J=8.4, fluorophenoxy)pyridin-4-yl)-4- 1.6Hz), 7.47 (s, IH), 7.15 (td, IH, J=9.2, (methylsulfonyl)-3,4-dihydro-2H- 1.6Hz), 7.34 (td, IH, J=8.4, 2.8Hz), 4.82-4.83 thieno [3 ,2-g] cbxomene-7 -carboxamide

(m, IH), 4.49-4.53 (m,lH), 4.32-4.35 (m, IH), 3.17 (s, 3H), 2.60-2.65 (m, IH), 2.36-2.39 (m, IH)

LC/MS ESI (+): 561 (M+l)

'H-NMR (400MHz, OMSO-d₆): 5 10.92 (s,

N-(2-chloro-6-(5-chloro-2- IH), 8.30 (s, IH), 8.07 (s, IH), 7.64 (d, IH, methyIphenoxy)pyridin-4-yl)-4- 7=1.2Hz), 7.58 (s, IH), 7.40 (d, IH, J=7.8Hz), (methylsulfonyl)-3,4-dihydro-2H- 7.28 (m, 3H), 4.81 (m, IH), 4.44-4.52 (m, thieno[3,2-g]chromene-7 -carboxamide

IH), 4.30-4.34 (m, IH), 3.16 (s, 3H), 2.59- 2.64 (m, IH), 2.34-2.39 (m, IH), 2.11 (s, 3H)

LC/MS ESI (+): 563 (M+l)

N-(2-chloro-6-(3 -chloro-4- 1 H-NMR (400MHz, DMSO- ₆): δ 10.86 (brs, methylphenoxy)pyridin-4-yl)-4- IH), 8.23 (s, IH), 7.99 (s, IH), 7.59 (s, IH), (methylsulfonyl)-3,4-dihydro-2H- 7.51 (s, IH), 7.38 (d, IH, J=8.3Hz), 7.29 (s, thieno [3 ,2-g] chromene-7 -carboxamide

IH), 7.20 (s, IH), 7.05 (d, IH, J=8.3Hz), 4.74- 4.76 (m, 1Η), 4.38-4.44 (m, 1Η), 4.24-4.26

(m, 1Η), 3.09 (s, 3Η), 2.53-2.60 (m, 1Η), 2.30 (s, 3Η), 2.27-2.32 (m, 1Η)

LC/MS ESI (+): 583 (M+l)

Ή-NMR (400MHz, DMSO-</₆): δ 10.90 (brs,

N-(2-chloro-6-(2- 1H), 8.25 (s, 1H), 8.00 (s, 1H), 7.78 (d, 1H,

(trifluoromethyl)phenoxy)pyridin-4-yl)- J=7.8Hz), 7.72 (t, 1H, J=7.8Hz), 7.59 (s, 1H),

39

4-(methylsulfonyl)-3,4-dibydro-2H- 7.51 (s, 1H), 7.38-7.44 (m, 2H), 7.33 (s, 1H), thieno[3,2-g]chromene-7-carboxamide 4.74-4.76 (m, 1H), 4.38-4.44 (m, 1H), 4.24- 4.26 (m, 1H), 3.09 (s, 3H), 2.53-2.57 (m, 1H), 2.27-2.32 (m, 1H)

LC/MS ESI (+): 599 (M+l)

Ή-NMR (400MHz, DMSO-i¾: δ 10.91 (brs,

N-(2-chloro-6-(2-

1H), 8.23 (s, 1H), 8.00 (s, 1H), 7.57 (s, 1H),

(trifluoromethoxy)phenoxy)pyridin-4-yl)-

40 7.51 (s, 1H), 7.33-7.50 (m, 5H), 4.74-4.75 (m, 4-(methylsulfonyl)-3,4-dihydro-2H-

1H), 4.38-4.43 (m, 1H), 4.23-4.27 (m, 1H), thieno [3 ,2-g] chromene-7-carboxamide

3.09 (s, 3H), 2.53-2.57 (m, 1H), 2.27-2.32 (m,

1H)

LC/MS ESI (+): 547 (M+l)

'H-NMR (400MHz, DMSO-< ₆): δ 10.94 (s,

N-(2-chloro-6-(2-fluoro-3- 1H), 8.30 (s, 1H), 8.07 (s, 1H), 7.64 (d, 1H, methylphenoxy)pyridin-4-yl)-4- J=1.3Hz), 7.58 (s, 1H), 7.36 (d, 1H, J=l .lHz),

41

(rnethylsulfonyl)-3,4-dihydro-2H- 7.14-7.24 (m, 3H), 4.81 (m, 1H), 4.44-4.52 thieno [3 ,2-g] chromene-7-carboxamide (m, 1H), 4.30-4.34 (m, 1H), 3.16 (s, 3H), 2.59- 2.64 (m, 1H), 2.33-2.39 (m, 1H), 2.29 (s, 3H)

LC/MS ESI (+): 579 (M+l)

1 H-NMR (400MHz, DMSO-rf₆): δ 10.81 (brs,

N-(2-chloro-6-(4-chloro-2- 1H), 8.21 (s, 1H), 7.98 (s, 1H), 7.53 (s, 1H), methoxyphenoxy)pyridin-4-yl)-4- 7.50 (s, 1H), 7.22 (d, 1H, J=2.3Hz), 7.17 (d,

42

(methylsulfonyl)-3,4-dihydro-2H- 1H, J=8.4Hz), 7.14 (s, 1H), 7.01 (dd, 1H, thieno[3,2-g]chromene-7-carboxamide J=8.4, 2.3Hz), 4.73-4.74 (m, 1H), 4.38-4.44

(m, 1H), 4.23-4.27 (m, 1H), 3.69 (s, 3H), 3.09 (s, 3H), 2.52-2.57 (m, 1H), 2.20-2.27 (m, 1H)

Examples 43 and 44) Separation of (S)-iV-(2-chloro-6-(4- chlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2- g]chromene-7-carboxamide and (R)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4- (methylsulfonyl)-3,4-dihydro-2H-thieno [3,2-g] chromene-7-carboxamide from rac-A^- (2-chloro-6-(4-chIorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4-dihydro-2H- thieno [3,2-g] chromene-7-carboxamide The racemate of N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g-]chromene-7-carboxamide (100.0 mg, 0.18 mmol) obtained in Example 8 was separated by preparative HPLC (Daicel Chiralpak IA, dichloromethane/ethanol=98/2, l O.OmL/min, 254nm, 35°C) into (5)-N-(2-chloro-6-(4- chlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene- 7-carboxamide (45.0 mg, 45%) and (i?)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide (44.0 mg, 44%). Example 43) (₁S)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-

(methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide

LC/MS ESI (+): 549 (M+l).

1H-NMR (400MHz, DMSO-d₆): 10.96 (s, 1H), 8.31 (s, 1H), 8.06 (s, 1H), 7.66 (s, 1H), 7.58 (s, 1H), 7.52 (d, 2H, J=8.80Hz), 7.31 (s, 1H), 7.27 (d, 2H, J=8.80Hz), 4.82 (m, 1H), 4.45-4.51 (m, 1H), 4.30-4.34 (m, 1H), 3.16 (s, 3H), 2.60-2.64 (m, 1H), 2.29-2.39 (m, 1H).

HPLC: Daicel Chiralpak IA, 0.46 cm I.D. x 15 cm L, dichloromethane/ethanol=98/2, l .OmL/min, 254nm, 35°C, IR= 3.08 min. Example 44) (i?)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-

(methylsulfonyl)-3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide

LC/MS ESI (+): 549 (M+l).

1H-NMR (400MHz, DMSO-< ₆): 11.03 (s, 1H), 8.32 (s, 1H), 8.06 (s, 1H), 7.66 (s, 1H), 7.57 (s, 1H), 7.52 (d, 2H, J=8.79Hz), 7.31 (s, 1H), 7.26 (d, 2H, J=8.79Hz), 4.82 (m, 1H), 4.45-4.51 (m, 1H), 4.30-4.33 (m, 1H), 3.16 (s, 3H), 2.60-2.64 (m, 1H), 2.30-2.39 (m, 1H).

HPLC: Daicel Chiralpak IA, 0.46 cm I.D. x 15 cm L, dichloromethane/ethanol=98/2, l .OmL/min, 254nm, 35°C, tR= 3.95 min. Compounds from Examples 45 to 49 were synthesized through the synthesis route of Example 43 and 44, and data of these compounds are listed as follows.

[Table 4]

1Η), 4.32-4.36 (m, 1Η), 3.24 (s, 3Η), 2.87- 2.94 (m, 1Η), 2.61-2.71 (m, 1Η)

LC/MS ESI (+): 567 (M+1)

Ή-NMR (400MHz, DMSO-i/₆): δ 10.91 (brs,

(R)-N-(2-chloro-6-(4- 1H), 8.26 (s, 1H), 8.23 (s, 1H), 7.63 (s, 1H), chlorophenoxy)pyridin-4-yl)-4-fluoro-4-

46 7.57 (s, 1H), 7.46 (d, 2H, J=8.7Hz), 7.21 (s, (methylsulfonyl)-3,4-dihydro-2H- 1H), 7.19 (d, 2H, J=8.7Hz), 4.46-4.49 (m, thieno [3 ,2-g] chromene-7 -carboxamide

1H), 4.31-4.37 (m, 1H), 3.24 (s, 3H), 2.86- 2.90 (m, 1H), 2.64-2.70 (m, 1H)

LC/MS ESI (+): 563 (M+l)

Ή-NMR (400MHz, DMSO-rf₆): δ 10.94 (brs,

(5)-N-(2-chloro-6-(4- 1H), 8.29 (s, 1H), 8.25 (s, 1H), 7.65 (s, 1H), chlorophenoxy)pyridin-4-yl)-4-methyl-4-

47 7.57 (s, 1H), 7.52 (d, 2H, J=8.5Hz), 7.30 (s, (methylsulfonyl)-3,4-dihydro-2H- 1H), 7.25 (d, 2H, J=8.3Hz), 4.49-4.54 (m, thieno [3 ,2-g] chromene-7-carboxamide

1H), 4.20-4.24 (m, 1H), 2.98 (s, 3H), 2.62 (m, 1H), 2.15-2.22 (m, 1H), 1.84 (s, 3H)

LC/MS ESI (+): 563 (M+l)

Ή-NMR (400MHz, DMSO-< ₆): δ 10.94 (brs,

(R)-N-(2-chloro-6-(4- 1H), 8.29 (s, 1H), 8.25 (s, 1H), 7.65 (s, 1H), chlorophenoxy)pyridin-4-yl)-4-methyl-4-

48 7.57 (s, 1H), 7.52 (d, 2H, J=8.5Hz), 7.30 (s, (methylsulfonyl)-3,4-dihydro-2H- 1H), 7.25 (d, 2H, J=8.3Hz), 4.49-4.54 (m, thieno[3,2-g]chromene-7-carboxamide

LC/MS ESI (+): 580 (M+l)

'H-NMR (400MHz, DMSO- ₆): δ 10.86 (brs,

(5)-N-(2-chloro-6-(3 -chloro-5 - 1H), 8.23 (s, 1H), 7.99 (s, 1H), 7.62 (s, 1H), methoxyphenoxy)pyridin-4-yl)-4- 7.51 (s, 1H), 7.22 (s, 1H), 6.89-6.90 (m, 1H),

49

(methylsulfonyl)-3,4-dihydro-2H- 6.85-6.86 (m, 1H), 6.74-6.76 (m, 1H), 4.74- thieno [3 ,2-g] chromene-7 -carboxamide 4.75 (m, 1H), 4.39-4.44 (m, 1H), 4.23-4.27

(m, 1H), 3.73 (s, 3H), 3.09 (s, 3H), 2.52-2.57 (m, 1H), 2.28-2.32 (m, 1H)

Examples 50) Synthesis of N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-l- (methylsulfonyl)-l,2,3,4-tetrahydrothieno[2,3-^]quinoxaline-7-carboxamide tert-butyl 7-((2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)carbamoyl)- 1 -

(methylsulfonyl)-2,3-dihydrothieno[2,3-g]quinoxaline-4(lH)-carboxylate (7.0 mg, 10.78 pmol) was dissolved in C¾C1₂ (108.0 μΐ), and TFA (300 μΐ, 3.89 mmol) was added at 20°C. The reaction mixture was stirred at room temperature for 2 hours. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN : 0.1% formic acid in H₂0) to obtain N-(2-chloro-6-(4- chlorophenoxy)pyridin-4-yl)-l-(methylsulfonyl)-l ,2,3,4-tetrahydrothieno[2,3- g]quinoxaline-7-carboxamide (2.5 mg, 42 %) as white amorphous.

Examples 51) Synthesis of N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-5- (methylsulfonyl)-8-oxo-5,6,7,8-tetrahydronaphtho[2,3-6]thiophene-2-carboxamide

To a solution of N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-5- (methylsulfonyl)-5,6,7,8-tetrahydronaphtho[2,3-b]thiophene-2-carboxamide (20.0 mg, 0.04 mmol) in acetic anhydride (1.0 ml, 10.58 mmol) was added chromium oxide(VI) (11.0 mg, 0.11 mmol) at 0°C. The reaction mixture was stirred at 0°C for 2 hours. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN : 0.1% formic acid in H₂0) to obtain N-(2-chloro-6-(4- chlorophenoxy)pyridin-4-yl)-5-(methylsulfonyl)-8-oxo-5,6,7,8-tetrahydronaphtho[2,3- 0]thiophene-2-carboxamide (5.0 mg, 24 %) as a white amorphous.

LC/MS ESI (+): 561 (M+l)

Ή-NMR (400MHz, OMSO-d₆): δ 1 1.14 (s, 1H), 8.72 (s, 1H), 8.46 (s, 1H), 8.23 (s, 1H), 7.68 (d, 1H, J=1.2Hz), 7.52 (d, 2H, J=8.8Hz), 7.33 (d, 1H, J=1.2Hz), 7.26 (d, 2H, J=8.8Hz), 4.99 (m, 1H), 3.17 (s, 3H), 3.02-3.09 (m, 1H), 2.79-2.83 (m, 1H), 2.61-2.67 (m, 2H)

Examples 52) Synthesis of N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4- (lH-pyrazol-l-yl)-3,4-dihydro-2H-thieno[3,2-^]chromene-7-carboxamide

To a suspension of 4-(lH-pyrazol-l-yl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7- carboxylic acid (17.0 mg, 0.06 mmol) in CH₂C1₂ (0.3 ml) were added (COCl)₂ (9.6 μΐ, 0.11 mmol) and DMF (0.4 μΐ, 5.66 μιτιοΐ). The reaction mixture was stirred at 40°C for lhour, and concentrated under reduced pressure to obtain 4-(lH-pyrazol-l-yl)-3,4-dihydro-2H- thieno[3,2-g]chromene-7-carbonyl chloride. To the residue, 2-chloro-6-(4- chlorophenoxy)pyridin-4-amine (28.9 mg, 0.11 mmol) and 1,4-dioxane (0.3 ml) was added. The reaction mixture was stirred at 80°C overnight. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN : 0.1% formic acid in H₂0) to obtain N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-(lH- pyrazol-l-yl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide (21.0 mg, 69 %) as a white solid.

LC/MS ESI (+): 537 (M+l)

Ή-NMR (400MHz, DMSO-c/₆): δ 10.77 (brs, 1H), 8.08 (s, 1H), 7.74 (d, 1H, J=2.0Hz), 7.55 (s, 1H), 7.43-7.48 (m, 4H), 7.33 (s, 1H), 7.16-7.20 (m, 3H), 6.23-6.25 (m, 1H), 5.80 (t, 1H, J=6.2Hz), 4.27-4.3 (m, 2H), 2.31-2.43 (m, 2H) Compounds from Examples 53 to 55 were synthesized through the synthesis route of Example 52, and data of these compounds are listed as follows.

[Table 5]

Examples 56) Synthesis of N-(3-chIoro-5-(2-(4-chIorophenyI)propan-2- yI)phenyl)-l-(methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2- b] [l,4]oxazine-7-carboxamide

(a) Synthesis of l-chloro-3-nitro-5-(prop-l-en-2-yl)benzene

To a suspension of l-bromo-3-chloro-5-nitrobenzene (7.2 g, 30.59 mmol), 4,4,5,5- tetramethyl-2-(prop-l-en-2-yl)-l,3,2-dioxaborolane (5.1 g, 30.59 mmol) and Na₂C0₃ (9.7 g, 91.76 mmol) in DME (120.0 mL)/H₂0 (30.0 mL) was added Pd(PPh₃)₄ (1.8 g, 1.53 mmol). The reaction mixture was refluxed overnight. Pd(PPh₃)4 (0.7 g, 0.61 mmol) was more added and stirred for 4hours. After cooling to room temperature, the reaction mixture was filtered through celite. The filtrate was concentrated and the residue was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na₂S0₄, filtered and concentrated. The residue was purified by NH-silica column chromatography (hexane only) to give l-chloro-3-nitro-5-(prop-l-en-2-yl)benzene (6.3 g) as a crude yellow oil.

1H-NMR (400MHz, CDC1₃): δ 8.19 (t, 1H, J=1.7Hz), 8.11 (t, 1H, J=1.8Hz), 7.74 (t, lH, J=1.7Hz), 5.30 (s, 1H), 2.19 (s, 3H) (b) Synthesis of l-(2-bromopropan-2-yl)-3-chloro-5-nitrobenzene

To a solution of l-chloro-3-nitro-5-(prop-l-en-2-yl)benzene (6.3 g, 22.14 mmol) in Et₂0 (100.0 n L) was added 33wt% HBr in ACN (38.8 mL, 221.38 mmol). The reaction mixture was stirred at room temperature for 2 days. Sat.NaHC0₃ aqueous solution was added under ice bath and the resulting mixture was extracted with Et₂0. The organic extract was washed with sat. NaHC0₃ aqueous solution and brine, dried over anhydrous Na₂S0₄, filtered and concentrated. The residue was purified by flash column chromatography (silica gel, «-Hex : EtOAc = 19 : 1) to obtain l-(2-bromopropan-2-yl)-3- chloro-5-nitrobenzene (5.3 g, 62% in 2 steps) as an ivory solid.

Ή-NMR (400MHz, CDC1₃): δ 8.34 (t, 1H, J=1.9Hz), 8.13 (t, 1H, J=1.9Hz), 7.26 (t, lH, J=1.8Hz), 2.21 (s, 6H)

(c) Synthesis of l-chloro-3-(2-(4-chlorophenyl)propan-2-yl)-5 -nitrobenzene l-(2-Bromopropan-2-yl)-3-chloro-5-nitrobenzene (2.0 g, 7.18 mmol) and chlorobenzene (10.9 mL, 0.11 mol) were dissolved in 1 ,2-dichloroethane (70.0 mL), and A1C1₃ (2.9 g, 21.54 mmol) was added. The reaction mixture was stirred at 0°C for 2 hours, H₂0 was added, and extracted with CH₂C1₂. The organic extract was washed with brine, dried over anhydrous Na₂S0₄ and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, 0.1% formic acid in CH₃CN: 0.1% formic acid in H₂0) to obtain l-chloro-3-(2-(4-chlorophenyl)propan-2- yl)-5 -nitrobenzene (1.95 g, 88%) as a yellow oil.

Ή-NMR (400MHz, CDC1₃): 5 8.06 (t, 1H, J=1.8Hz ), 7.99 (t, 1H, J=1.9Hz ), 7.47 (t, 1H, J=1.7Hz ), 7.29 (d, 2H, J=8.5Hz), 7.12 (d, 2H, J=8.5Hz), 1.70 (s, 6H)

(d) Synthesis of 3-chloro-5-(2-(4-chlorophenyl)propan-2-yl)aniline

l-chloro-3-(2-(4-chlorophenyl)propan-2-yl)-5 -nitrobenzene (1.95 g, 6.28 mmol) was dissolved in MeOH/THF/H₂0 (65.0 mL, 10/2/1 v/v), and Zn (6.17 mg, 94.3 mmol) and NH₄CI (1.68 g, 31.4 mmol) were added at room temperature. The reaction mixture was ultrasonificated at 40°C for 90 minutes, cooled to room temperature, filtered through Celite, and concentrated under reduced pressure. The residue was purified by flash column chromatography (amine silica gel, «-Hex : EtOAc = 9 : 1) to obtain 3-chloro-5-(2-(4- chlorophenyl)propan-2-yl)aniline (1.49 g, 85%) as a yellow oil.

LC/MS ESI (+): 280 (M+1)

Ή-NMR (400MHz, CDC1₃): δ 7.22 (d, 2H, J=8.7Hz), 7.14 (d, 2H, J=8.7Hz), 6.60 (t, 1H, J=1.7 Hz ), 6.50 (t, 1H, J=1.9 Hz ), 6.32 (t, 1H, J=1.9 Hz ),3.64 (s, 2H), 1.59 (s, 6H) (e) Synthesis of 7V-(3-chloro-5-(2-(4-chlorophenyl)propan-2-yl)phenyl)-l- (methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxamide

The synthesis procedure of Example 1-b was repeated except for using 3-chloro-5- (2-(4-chlorophenyl)propan-2-yl)aniline (12.1 mg, 0.04 mmol) to obtain N-(3-chloro-5-(2- (4-chlorophenyl)propan-2-yl)phenyl)- 1 -(methylsulfonyl)-2,3 -dihydro- 1H- thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxamide (12.8 mg, 77%) as a white solid.

LC/MS ESI (+): 575 (M+l)

1H-NMR(400MHz, DMSO-i ₆): δ 10.47 (s, 1H), 8.24 (s, 1H), 8.16 (s, 1H), 7.89 (s, 1H), 7.65 (s, 1H), 7.50 (t, 1H, J=1.6Hz), 7.39 (d, 2H, J=8.6Hz), 7.28 (d, 2H, J=8.6Hz), 7.03 (m, 1H), 4.37 (t, 2H, J=4.2Hz), 3.91 (t, 2H, J=4.4Hz), 3.19 (s, 3H), 1.66 (s, 6H).

Experimental Examples

Experiments were performed as shown below for the compounds prepared in Examples above.

Experimental Example 1) Experiment on the inhibition of STAT3 and STAT1 activities via reporter gene assay

1-1) Experiment on the inhibition of STAT3 activity

A human prostate cancer cell line (LNCaP stable cell line; plasmid pSTAT3-TA- luc), which contains a stably operating STAT3 promoter, was cultured in RPMI1640 medium (Cat No. 11875, Life Technologies) containing 10% fetal bovine serum (FBS) (Cat No. SH30396, Thermo Scientific) and 150 μg/mL G-418 solution (Cat No. 04 727 894 001, Roche). The reporter gene assay using LNCaP stable cell line was performed in RPMI1640 medium containing 3% DCC-FBS without G-418 solution. LNCaP stable cells were plated in two (2) white 96- well plates with 30,000 cells/50 in each well. The cells were cultured at 37°C, under 5% C0₂ for 24 hours, and then treated with the compounds listed in Examples which were diluted in various concentrations. Subsequently, IL-6 was added to each well with a final concentration of 10 ng/mL. Upon completion of the treatment with the compounds and IL-6, the cells were cultured at 37°C, under 5% C0₂ for 24 hours. The plates were observed under microscope and drug precipitation and particular findings were investigated and recorded.

The luciferase assay and the cell viability assay were performed respectively with one of the two plates. For the luciferase assay, the liquid media in the 96-well plate was removed, and then, 20 of passive cell lysis buffer was added to each well. After shaking the plate for 30 minutes, luciferase activities of each well were measured in a PHERAstar™ microplate reader (BMG LABTECH) using a luciferase assay system (Cat No. E1501, Promega). For the cell viability assay, the 96-well plate was placed at room temperature for 30 minutes, added with 20 μΙ,ΛνεΙΙ of CellTiter-Glo solution (Cat No. G7573, Promega), and shaken for 10 minutes in order to measure cytotoxicity caused by the compounds listed in Examples with a PHERAstar™ microplate reader (BMG LABTECH). Wells without 0.1% DMSO and stimulation were used as a negative control and wells with 0.1% DMSO and stimulation were used as a positive control.

1-2) Experiment on the inhibition of STATl activity

A human osteosarcoma cell line (U20S stable cell line; pGL4-STATl -TA-luc), which contains a stably operating STATl promoter, was cultured in McCoy 5Ά medium (Cat No. 16600, Life Technologies) containing 10% FBS (Cat No. SH30396, Thermo Scientific) and 1000 μg /mL G418 solution (Cat No. 04 727 894 001 , Roche). The reporter gene assay using U20S stable cell line was performed in McCoy 5Ά medium containing 10% FBS without G-418 solution. U20S stable cells were plated in two (2) white 96-well plates with 25,000 cells/50 in each well. The cells were cultured at 37°C, under 5% C0₂ for 24 hours, and then treated with the compounds listed in Examples which were diluted in various concentrations. Subsequently, IFN-γ was added to each well with a final concentration of 50 ng/mL. Upon completion of the treatment with the compounds and IFN-γ, the cells were cultured at 37°C, under 5% C0₂ for 8 hours. The plates were observed under microscope and drug precipitation and particular findings were investigated and recorded.

The luciferase assay and the cell viability assay were performed respectively with one of two plates. For the luciferase assay, the liquid media in the 96-well plate was removed, and then, 20 μΐ, of passive cell lysis buffer was added to each well. After shaking the plate for 30 minutes, luciferase activities of each well were measured in a PHERAstar™ microplate reader (BMG LABTECH) using a luciferase assay system (Cat No. El 501, Promega). For the cell viability assay, the 96-well plate was placed at room temperature for 30 minutes, added with 20 μΤΛνεΙΙ of CellTiter-Glo solution (Cat No. G7573, Promega), and shaken for 10 minutes in order to measure cytotoxicity caused by the compounds listed in Examples with a PHERAstar™ microplate reader (BMG LABTECH). Wells without 0.1% DMSO and stimulation were used as a negative control and wells with 0.1 % DMSO and stimulation were used as a positive control.

The results of evaluation on the inhibitory effect of the compounds listed in the Examples on the dimerization of STAT3 and STATl obtained via the STAT3 and STATl reporter gene assays are shown in Table 6 below.

[Table 6]

Ex. ICso (μΜ) ICso (μΜ) IC₅₀ (μΜ) ICso (μΜ)

Ex.

pSTAT3 pSTATl pSTAT3 pSTATl

1 0.0026 >50 2 0.17 >50 3 0.01 >50 4 0.93 >50

5 0.76 >50 6 0.0030 >50

7 0.012 >50 8 0.024 >50

9 0.029 >50 10 0.0026 >50

11 0.0060 >50 12 0.0030 >50

13 0.0081 >50 14 0.029 >50

15 0.018 >50 16 0.0051 >50

17 0.0039 >50 18 0.015 >50

19 0.0084 >50 20 0.010 >50

21 0.011 >50 22 0.030 >50

23 0.0063 >50 24 0.0062 >50

25 0.011 >50 26 0.0073 >50

27 0.0078 >50 28 0.0051 >50

29 0.0059 >50 30 0.02 >50

31 0.008 >50 32 0.016 >50

33 0.0098 >50 34 0.0064 >50

35 0.010 >50 36 0.014 >50

37 0.0062 >50 38 0.0088 >50

39 0.014 >50 40 0.0087 >50

41 0.011 >50 42 0.023 >50

43 0.018 >50 44 1.5 >50

45 0.0039 >50 46 0.14 >50

47 0.0045 >50 48 0.15 >50

49 0.0066 >50 50 0.0082 >50

51 0.10 >50 52 0.10 >50

53 5.2 >50 54 0.18 16.6

55 0.039 17.9 56 0.0028 >50

As shown in Table 6, the compounds according to the present invention exhibited excellent inhibitory effects against the activity of STAT3 protein but showed almost no inhibitory effect against the activity of STAT1 protein.

Experimental Example 2) Cell growth inhibition assay

The inhibitory effects of the compounds of the present invention against the growth of cancer cells were evaluated as shown below. The cancer cell lines including stomach cancer cell line (NCI-N87) and breast cancer cell line (MDA-MB-468) were cultured under the protocol provided by each supplier. Each type of cells to be used in experiments was sub-cultured in a 96-well plate by counting the exact number of cells using Tali Image- based Cytometer (Life Technologies). In a 96-well plate, NCI-N87 was employed with 5,000 cells/well; and MDA-MB-468 was employed with 10,000 cells/well. The cells were treated with the compounds listed in Examples which were diluted in various concentrations. Upon completion of the compounds treatment, NCI-N87 cells were cultured at 37°C under 5% C0₂ for 96 hours, and MDA-MB-468 cells were cultured at 37°C in air for 96 hours. Subsequently, the cells were observed under microscope and drug precipitation and particular findings were investigated and recorded. And then, the 96- well plate was placed at room temperature for 30 minutes, added with 20 μίΛ εΙΙ of CellTiter-Glo solution (Cat No. G7573, Promega) and shaken for 10 minutes, followed by being subjected to the measurement using PHERAstar™ microplate reader (BMG LABTECH) according to the supplier's general luminometer protocol. Wells where only culture liquid added without cell plating were used as a negative control, whereas wells where culture liquid containing 0.1% DMSO instead of the compounds listed in Examples were used as a positive control.

The results of the inhibitory effects of the compounds prepared in Examples against the growth of cancer cells are shown in Tables 7 to 8 below. [Table 7]

[Table 8]

7 0.0065 8 0.0032 9 0.023 10 0.0019

11 0.0052 12 0.0028 13 0.0066 14 0.026

15 0.0089 16 0.0039 17 0.0023 18 0.0043

19 0.0025 20 0.0024 21 0.0051 22 0.017

23 0.0028 24 0.0024 25 0.0049 26 0.0046

27 0.0029 28 0.0020 29 0.0029 30 0.0085

31 0.0022 32 0.0089 33 0.0093 34 0.0023

37 0.0047 40 0.0086 41 0.012 42 0.0078

43 0.0029 44 0.086 45 0.0025 46 >0.10

47 0.0017 48 >0.10 49 0.0059 51 0.050

52 0.12 53 0.71 54 0.075 55 0.078

As shown in Tables 7 to 8, the compounds according to the present invention exhibited excellent inhibitory effects against the growth of various kinds of cancer cells.

Claims

WHAT IS CLAIMED IS:

1. A compound selected from the group consisting of a heterocyclic derivative represented by formula (I), and a pharmaceutically acceptable salt and a stereoisomer thereof:

wherein

X_! and X₂ are each independently -C(-Rx)(-Rx")-, -C(-Rx')(-Rx")-, -C(-Rx")(- Rx")-, -C(=0)-, -N(Rx)-, -N(-Rx')-, -N(-Rx")-, or -0-;

O

■I I

Rs— S— I

II s

Rx is ^Xs ;

Xs is =0 or =NH;

Rs is C_1-6alkyl, haloC_1-6alkyl, C_1-6alkoxy-C_1- alkyl, Ci_-6alkylcarbonyl-C_1-6alkyl, C_2-7alkenyl, amino, or aminoCi_-6alkyl;

Rx' is haloC_1-6alkyl, C_1-4alkoxycarbonyl, cyano, nitro, azido, amino, or a 3-6 membered heterocyclyl unsubstituted or substituted with Rx";

Rx" is each independently hydrogen, halogen, nitro, amino, C_1-6alkyl, Ci_-6alkoxy, haloC_1-6alkoxy, carbamoylC_1-6alkyl, C_1-6alkylamino-C_1-6alkyl, or diCi-ealkylamino-Ci. ealkyl;

one of Y and Z is -S- or -NH-, and the other is -CH= or -N=;

A and B are each independently a monocyclic- or bicyclic-saturated or unsaturated C_3-10carbocycle or 5- to 12-membered heterocycle;

Rc is =0, =NH, =N(-C_1-6alkyl), or =N(-OH);

R_N is hydrogen or C_1- alkyl;

L_B is -[C(-R_L)(-R_L')]_m-, -[C(-R_L)(-R_L')]_n-0-, -0-, -NH-, -N(C_1-6alkyl)-, -S(=0)₂-, - C(=0)-, or -C(=CH₂)-, wherein m is an integer of 0 to 3, n is an integer of 1 to 3, RL and RL' are each independently hydrogen, hydroxy, halogen or Ci_-6alkyl, or RL and RL' are linked together to form Ci_-6alkylene;

RA is hydrogen, halogen, cyano, C_1-6alkyl, haloC^alkyl, cyanoCi_-6alkyl, C_\. ₆alkylcarbonyl, C_1- alkoxy, haloC_1-6alkoxy, cyanoC_1-6alkoxy, d-ealkylamino, diC]. ₆alkylamino, Ci_-6alkylthio, Ci_₆alkylaminocarbonyl, diCi_-6alkylaminocarbonyl, C_2- ₈alkynyl, Q-ealkoxycarbonylamino-Ci^alkoxy, aminoCi_-6alkoxy, or 3- to 6-membered heterocyclyl;

RB is hydrogen, halogen, hydroxy, cyano, nitro, amino, oxo, aminosulfonyl, sulfonylamido, Ci_-6alkyl amino, Ci_-6alkyl, haloC]-₆alkyl, cyanoC_{] -} alkyl, Ci- alkoxy, haloC_1-6alkoxy, cyanoC_1-6alkoxy, C_3-8cycloalkyloxy, C_2-8alkenyl, C_2-8alkenyloxy, C_2- ₈alkynyl, C_2-8alkynyloxy, C_1-6alkylamino-C_1-6alkoxy, diCi^alkylamino-Ci-ealkoxy, C_\. ₆alkoxy-carbonyl, carbamoyl, carbamoyl-C_1- alkoxy, C_{1 -6}alkylthio, C_1-6alkylsulfinyl, Ci. ₆alkylsulfonyl, 5- to 10-membered heterocyclyl, 5- to 10-membered heterocyclyl-C_1-6alkyl, 5- to 10-membered heterocyclyl-Ci_-6alkoxy, or 5- to 10-membered heterocyclyl-oxy;

q is an integer of 0 to 4, and, when q is 2 or higher, R_B moieties are the same as or different from each other; and

2. The compound according to claim 1 , wherein

one of Y and Z is -S- or -NH-, and the other is -CH=;

Lx is a saturated Ci_-3 hydrocarbon chain not containing or containing at least one heteroatom selected from the group consisting of O, N and S in the chain, and unsubstituted or substituted with at least one substituent selected from the group consisting of halogen, C_1-6alkyl and C_1-6alkoxy;

O

Rs— S— I

II ^¾

Rx is ^Xs ;

Xs is =0 or =NH;

Rs is Ci_-6alkyl or

Rx' is halod^alkyl, cyano, nitro, amino, azido, or a 5- to 6-membered heterocyclyl containing at least one heteroatom selected from the group consisting of N, S and O and unsubstituted or substituted with oxo; and

Rx" is hydrogen, halogen, Ci_-6alkyl, or C_1-4alkoxycarbonyl.

3. The compound according to claim 2, wherein

Y is -CH=; Rc is =0;

RN is hydrogen;

Lx is a saturated C_1-3 hydrocarbon chain not containing or containing oxygen atom in the chain, and unsubstituted or substituted with at least one substituent selected from the group consisting of halogen, C_1-6alkyl and C_1-6alkoxy;

X, is -C(-Rx)(-Rx")-, -C(-Rx')(-Rx")-, or -N(Rx)-;

X₂ is -C(-Rx")(-Rx")-, -C(=0)-, -N(-Rx")-, or -0-;

O

H₃C-S— I

J II s

Rx is ^Xs ;

Xs is =0 or =NH;

Rx' is haloC_1-6alkyl, cyano, nitro, amino, azido, or a 5- to 6-membered heterocyclyl containing 1 to 2 heteroatoms selected from N and O and unsubstituted or substituted with oxo; and

Rx" is hydrogen, halogen, C_1-6alkyl, or C^alkoxycarbonyl.

4. The compound according to claim 3, wherein

B is a monocyclic- or bicyclic-saturated or unsaturated C_6-iocarbocycle or 5- to 10- membered heterocycle;

L_B is -[C(-R_L)(-RL')]m-, -0-, -NH- or -N(Ci_-6alkyl)-, wherein m is 0 or 1, RL and R_L' are each independently hydrogen, hydroxy, halogen or C_1-6alkyl, or RL and R_L' are linked together to form C_2-5alkylene;

RA is halogen, d-ealkoxycarbonylamino-C_t-ealkoxy, aminoCi_-6alkoxy, or 3- to 6- membered heterocyclyl;

R_B is halogen, C_1- alkyl, C_1-6alkoxy, haloC_1-6alkyloxy, C_2-6alkenyloxy, C_3- _locarbocyclyl-oxy, or 3- to 10-membered heterocyclyl-Ci_-3alkoxy; and

5. The compound according to claim 1, wherein

X₂ is -C(-Rx")(-Rx")- or -N(-Rx")-;

Y is -CH=;

Z is -S-;

Rc is =0;

R_N is hydrogen; Lx is ethylene substituted with one or two Rx" moieties, O

Rs— S— 1

II s

Rx is ^Xs ;

Xs is =0;

Rs is methyl; and

Rx" is the same as defined in claim 1.

The compound according to claim 1 , wherein

Xi is -CH(-Rx)-;

X₂ is -N(-Rx")-;

Y is -CH=;

Z is -S-;

Rc is =0;

RN is hydrogen;

Lx is ethylene;

O

II S

Rs-S— I

II «

Rx is ^Xs ;

Xs is =0;

Rs is methyl; and

Rx" is the same as defined in claim 1.

The compound according to claim 1, wherein

X, is -C(-Rx)(-Rx")-;

X₂ is -0-;

Y is -CH=;

Z is -S-;

Rc is =0;

RN is hydrogen;

Lx is ethylene;

O

II S

Rs-S— I

II s

Rx is ^Xs ;

Xs is =0;

Rs is methyl; and

Rx" is the same as defined in claim 1.

8. The compound according to claim 1, wherein X, is -C(-Rx')(-Rx")-;

X₂ is -0-;

Y is -CH=;

Z is -S-;

Rc is =0;

R_N is hydrogen;

Lx is ethylene; and

Rx' and Rx" are the same as defined in claim 1.

9. The compound according to claim 1 , wherein X_! is -CH(-Rx)-;

X₂ is -C(-Rx")(-Rx")- or -C(=0)-;

Y is -CH=;

Z is -S-;

Rc is =0;

R_N is hydrogen;

Lx is ethylene;

Xs is =0;

Rs is methyl; and

Rx" is the same as defined in claim 1.

The compound according to claim 1 , wherein

X₂ is -C(-Rx")(-Rx")-;

Y is -CH=;

Z is -S-;

Rc is =0;

R_N is hydrogen;

Lx is -CH₂-0-;

Xs is =0; Rs is methyl; and

Rx" is the same as defined in claim 1.

11. The compound according to claim 1 , wherein

Xi is -C(-Rx)(-Rx")- or -N(Rx)-;

X₂ is -0-;

Y is -NH-;

Z is -CH=;

Rc is =0;

R_N is hydrogen;

Lx is propylene; and

Rx and Rx" are the same as defined in claim 1.

12. The compound according to claim 1, which is selected from the group consisting of:

1 ) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)- 1 -(methylsulfonyl)-2,3 -dihydro- lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxamide;

2) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-3 ,3 -dimethyl- 1 - (methylsulfonyl)-2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxamide;

3) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-l -(methylsulfonyl)-l, 2,3,4- tetrahydrothieno[3',2':4,5]benzo[ 1 ,2-b][ 1 ,4]oxazepine-8-carboxamide;

4) jV-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-8,8-dimethyl-5- (methylsulfonyl)-5,6,7,8-tetrahydrothieno[2,3-g]quinoline-2-carboxamide;

5) tert-butyl 7-((2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)carbamoyl)- 1 - (methylsulfonyl)-2,3-dihydrothieno[2,3-g]quinoxaline-4(lH)-carboxylate;

6) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-5-(methylsulfonyl)-5, 6,7,8- tetrahydrothieno[2,3-g]quinoline-2-carboxamide;

7) 7V-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-methyl- 1 -(methylsulfonyl)- l,2,3,4-tetrahydrothieno[2,3-g]quinoxaline-7-carboxamide;

8) 7V-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4-dihydro- 2H-thieno[3,2-g]chromene-7-carboxamide;

10) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-5-(methylsulfonyl)-5, 6,7,8- tetrahydronaphtho[2,3-£]thiophene-2-carboxamide;

11 ) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-methyl-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

15) N-(2-chloro-6-(^-tolyloxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4-dihydro-2H- thieno[3,2-g]chromene-7-carboxamide;

16) N-(2-chloro-6-(3-(trifluoromethyl)phenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

17) N-(2-chloro-6-(4-(trifluoromethyl)phenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

18) N-(2-chloro-6-(3,5-dichlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

19) N-(2-chloro-6-(4-chloro-3-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

21) N-(2-chloro-6-(4-chloro-2-methylphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

25) V-(2-chloro-6-(3-chlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

27) N-(2-chloro-6-(3-chloro-4-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

28) N-(2-chloro-6-(4-(trifluoromethoxy)phenoxy)pyridin-4-yl)-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

29) 7V-(2-chloro-6-(3-(trifluoromethoxy)phenoxy)pyridin-4-yl)-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

31 ) N-(2-chloro-6-(3 -chloro-5-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

32) N-(2-chloro-6-(3-fluoro-5-methoxyphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

34) N-(2-chloro-6-(3,4-difluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H hieno[3,2-g]chromene-7-carboxamide;

35) N-(2-chloro-6-(5-chloro-2-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

36) N-(2-chloro-6-(3-chloro-2-fluorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

38) N-(2-chloro-6-(3-chloro-4-methylphenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

39) N-(2-chloro-6-(2-(trifluoromethyl)phenoxy)pyridin-4-yl)-4-(methylsulfonyl)- 3 ,4-dihydro-2H-thieno [3 ,2-g] chroniene-7-carboxamide;

44) (i?)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-(methylsulfonyl)-3,4- dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

45) (₁S)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-fluoro-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

46) (i?)- V-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-fluoro-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

47) (5)-N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-methyl-4- (methylsulfonyl)-3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

48) (/?)-7V-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-methyl-4- (methylsulfonyl)-3,4-dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

49) (₄S)-N-(2-chloro-6-(3-chloro-5-methoxyphenoxy)pyridin-4-yl)-4- (methylsulfonyl)-3 ,4-dihydro-2H-thieno [3 ,2-g] chromene-7-carboxamide;

52) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-( lH-pyrazol- 1 -yl)-3 ,4- dihydro-2H-thieno[3,2-g]chromene-7-carboxamide;

53) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-(2-oxopyrrolidin-l-yl)-3,4- dihydro-2H-thieno[3,2-g-]chromene-7-carboxamide;

54) N-(2-chloro-6-(4-chlorophenoxy)pyridin-4-yl)-4-cyano-3,4-dihydro-2H- thieno[3,2-g]chromene-7-carboxamide;

56) jV-(3 -chloro-5 -(2-(4-chlorophenyl)propan-2-yl)phenyl)- 1 -(methylsulfonyl)- 2,3-dihydro-lH-thieno[3',2':4,5]benzo[l,2-b][l,4]oxazine-7-carboxamide.

13. A pharmaceutical composition for preventing or treating diseases associated with the activation of STAT3 protein, comprising the compound as defined in any one of claims 1 to 12 as an active ingredient.

14. The pharmaceutical composition according to claim 13, wherein the diseases associated with the activation of STAT3 protein is selected from the group consisting of solid cancers, hematological or blood cancers, radio- or chemo-resistant cancers, metastatic cancers, inflammatory diseases, immunological diseases, diabetes, macular degeneration, human papillomavirus infection and tuberculosis.

15. The pharmaceutical composition according to claim 13, wherein the diseases associated with the activation of STAT3 protein are selected from the group consisting of breast cancer, lung cancer, stomach cancer, prostate cancer, uterine cancer, ovarian cancer, kidney cancer, pancreatic cancer, liver cancer, colon cancer, skin cancer, head and neck cancer, thyroid cancer, osteosarcoma, acute or chronic leukemia, multiple myeloma, B- or T-cell lymphoma, non-Hodgkin's lymphoma, auto-immune diseases comprising rheumatoid arthritis, psoriasis, hepatitis, inflammatory bowel disease, Crohn's disease, diabetes, macular degeneration, human papillomavirus infection, and tuberculosis.

16. A use of the compound as defined in any one of claims 1 to 12 for the manufacture of a medicament for preventing or treating diseases associated with the activation of ~ STAT3 protein.

17. A method for preventing or treating diseases associated with the activation of STAT3 protein in a mammal, which comprises administering the compound as defined in any one of claims 1 to 12 to the mammal