WO2016063569A1

WO2016063569A1 - Sample analysis device

Info

Publication number: WO2016063569A1
Application number: PCT/JP2015/067492
Authority: WO
Inventors: 啓綱澤; 中村　篤; 大河藤田
Original assignee: シャープ株式会社
Priority date: 2014-10-23
Filing date: 2015-06-17
Publication date: 2016-04-28
Also published as: JP2016085078A; JP5964388B2

Abstract

A sample analysis device (100) is provided with a chip (102) in which a reference chamber (414) accommodating a sample and a measurement chamber (404) accommodating a measurement liquid are formed on the same circumference of an axis of rotation (450) and with a measurement unit (109) for analyzing a component included in the sample on the basis of the amount of transmitted reference light that has passed through the reference chamber (414) and the amount of transmitted measurement light that has passed through the measurement chamber (404).

Description

Sample analyzer

The present invention relates to a sample analyzer, and more particularly to a sample analyzer suitable for analyzing soil components.

In the field of agriculture, analysis of soil components in the growing environment of crops is widely performed to manage the growing state of the crops.

Generally, a soil analyzer injects each soil extract into a plurality of test tubes while measuring with a graduated dropper, and then adds the reagent and diluent determined for each soil component to the test tubes. Inject and develop color. And it is measured by converting into a numerical value using a colorimetric table, a turbidimetric table, an absorptiometric method or the like.

However, the measurement method described above needs to mix a reagent with each soil extract, which increases the number of repetitive operations. Moreover, it is necessary to prepare a reagent according to the soil component to be measured, and the complexity is high.

By performing soil analysis frequently, it is possible to perform detailed analysis for each field and analysis for each planting. Therefore, it is possible to design a fertilizer application that takes into account the effects of the previous crop, and it is possible to optimize the timing and amount of additional fertilization by periodically analyzing the crops with a long growth period in a shorter span. As a result, an increase in yield and stabilization of quality can be expected.

However, it is difficult to increase the frequency of analysis due to the high complexity described above.

In recent years, in view of such problems, a method for analyzing soil components by mixing a soil extract and a reagent by a simple method has been proposed.

FIG. 11 shows a reagent mixing and soil analysis apparatus disclosed in Patent Document 1. FIG. 11 (a) is a schematic diagram of a conventional soil analysis apparatus, and FIG. 11 (b). And (c) is a schematic diagram showing the fitting between the storage cartridge provided in the conventional soil analyzer and the extract cartridge.

The soil analysis apparatus described in Patent Document 1 includes a light emitting unit 7, a light receiving unit 8, and a storage cartridge 9, as shown in FIG. The storage cartridge 9 is made of a transparent material, and is provided with a plurality of cells 11 for storing a mixture of a soil extract and a reagent extracted from soil. In the soil analyzer described in Patent Document 1, light emitted from the light emitting unit 7 passes through the mixed solution in the storage cartridge 9 and is detected by the light receiving unit 8 to measure the absorbance of the mixed solution. The concentration of soil components is measured by the method.

As shown in FIG. 11B, a predetermined amount of reagent is previously stored in the cell 11 of the storage cartridge 9 and is sealed with a seal paper 15. Each cell 16 of the extraction liquid cartridge 14 has a dredging function as measurement, and contains a soil component liquid. Before the measurement, the extract cartridge 14 is pushed into the storage cartridge 9 in the direction indicated by the arrows in FIGS. 11B and 11C, so that the storage cartridge 9 and the extract cartridge 14 are fitted. Thereafter, the bottom surface of the extraction liquid cartridge 14 is penetrated, and the extraction liquid is injected into the cell 11 of the storage cartridge 9 to prepare a mixed liquid.

Thus, in the soil analyzer described in Patent Document 1, it is easy to create a mixed solution, and the concentration of soil components is measured by the absorptiometric method, so that accurate measurement can be performed. .

Further, Patent Document 2 discloses an analysis device and an analysis apparatus in which a microchannel is formed. In the analysis apparatus described in Patent Document 2, centrifugal force acts on the analysis device by rotationally driving the analysis device in which the microchannel is formed. Thereby, the sample and the reagent held in the analytical device are moved to the reaction tank, and a mixed solution of the sample and the reagent is created by a simple method.

Japanese Patent Publication “Japanese Patent Laid-Open No. 2007-46922 (published on February 22, 2007)” Japanese Patent Publication “JP 2009-210564 A (published on September 17, 2009)”

Here, when analyzing components contained in the sample, the soil extract itself may be colored depending on the state of the soil. When measuring the concentration of the soil component by the absorptiometry using the analyzers described in the cited references 1 and 2 for such a soil extract, there is a problem that the measurement accuracy decreases. .

Also, in order for producers to measure soil components themselves, the measurement work is required to be simple and efficient.

The present invention has been made in view of the above-described problems, and an object of the present invention is to provide a sample analyzer that is simple and efficient in measurement work and can perform accurate measurement. .

In order to solve the above-described problem, a sample analyzer according to one aspect of the present invention includes a reference chamber that stores a sample and a measurement chamber that stores a measurement liquid based on the sample. A container formed above, a light emitting part that is arranged at a position corresponding to the same circumference and emits light toward the container, a light receiving part that receives light transmitted through the container, the container and the container A rotation driving unit that rotates at least one of the light emitting unit and the light receiving unit around the rotation axis, a reference transmitted light amount of light transmitted through the reference chamber and received by the light receiving unit, and transmitted through the measurement chamber. And a measuring unit for analyzing the components contained in the sample based on the measured transmitted light amount of the light received by the light receiving unit.

According to one aspect of the present invention, the reference chamber containing the sample and the measurement chamber containing the measurement liquid based on the sample are formed in the container on the same circumference around the rotation axis. Therefore, the measurement can be performed simply and efficiently by rotating the container and performing the measurement.

Further, the component contained in the sample is analyzed based on the reference transmitted light amount of the light transmitted through the reference chamber and received by the light receiving unit and the measured transmitted light amount of the light transmitted through the measurement chamber and received by the light receiving unit. Therefore, accurate measurement can be performed.

1 is a schematic configuration diagram of a sample analyzer according to Embodiment 1 of the present invention. It is the structure schematic of the light emission part with which the sample analyzer shown in FIG. 1 is equipped. FIG. 3 is a schematic configuration diagram of a filter array provided in the light emitting unit shown in FIG. 2. FIG. 2 is a schematic configuration diagram of a chip provided in the sample analyzer shown in FIG. 1, (a) is a front view of the chip as viewed from above, and (b) and (c) are the shapes of cells provided in the chip. FIG. (A) is a top view of the chip at the time of measurement, and (b) is a cross-sectional view of the chip taken along line BB in (a), and the sample analyzer shown in FIG. 1 schematically shows a filter array and a light receiving section. When the measurement is performed by rotating the chip shown in FIG. 4A at a constant speed and scanning it with the light emitted from the light emitting unit, the circumferential position of the chip and the amount of transmitted light received by the light receiving unit It is a graph which shows the relationship. FIG. 5 is a view showing a portion where a light shielding portion is provided in the chip shown in FIG. It is the structure schematic of the light emission part with which the sample analyzer based on Embodiment 2 of this invention is provided. It is the schematic which showed the filter array with which the light emission part shown in FIG. 8 is provided, (a) is the front view seen from upper direction, (b) is an enlarged view of the reference | standard filter with which a filter array is equipped. It is a graph which shows the relationship between a component density | concentration and a light absorbency in the case of measuring an absorbance previously using the soil extract whose component density | concentration is clear. (A) is a schematic diagram of a conventionally used soil analyzer, and (b) and (c) show the fitting between the storage cartridge provided in the soil analyzer shown in (a) and the extract cartridge. It is a schematic diagram shown.

[Embodiment 1]
Hereinafter, an embodiment of a sample analyzer according to the present invention will be described with reference to the drawings. In addition, the thickness, length, and the like of each constituent member in the drawings are shown to assist the understanding of the present invention, and the present invention is not limited to the illustrated configuration.

FIG. 1 shows a schematic configuration diagram of a sample analyzer 100 according to Embodiment 1 of the present invention. A sample analyzer 100 according to Embodiment 1 of the present invention is a sample analyzer that performs measurement by an absorptiometry. As shown in FIG. 1, a light emitting unit 101, a chip (container) 102, a light receiving unit 103, and the like. , A reference light receiving unit 104, a half mirror 108, a rotation driving unit 106, a measuring unit 109, and a control unit 111.

The configuration of each part will be described in detail below.

FIG. 2 is a schematic configuration diagram of the light emitting unit 101. As shown in FIG. 2, the light emitting unit 101 includes a plurality of

light sources

201a, 201b, and 201c having different emission wavelengths,

collimating lenses

202a, 202b, and 202c corresponding to the plurality of

light sources

201a, 201b, and 201c, and a dichroic.

Mirrors

203a and 203b, an aperture 204, and a filter array 205 are provided.

The light emitting unit 101 is connected to the control unit 111, and each of the light sources 201a to 201c is controlled in light emission, extinction, and light emission intensity by a signal from the control unit 111. In the present embodiment, a white LED (Light Emitting Diode) is used as the light source 201a, a blue LED is used as the light source 201b, and a red LED is used as the light source 201c.

The

dichroic mirrors

203a and 203b are mirrors that transmit light in a specific wavelength band and reflect light in another specific wavelength band. In the present embodiment, a dichroic mirror 203a that transmits light in the wavelength band of 470 nm to 1600 nm and reflects light in the wavelength band of 350 nm to 430 nm is used. The dichroic mirror 203b is a mirror that transmits light having a wavelength band of 400 nm to 630 nm and reflects light having a wavelength band of 675 nm to 850 nm.

FIG. 3 is a front view of the filter array 205 shown in FIG. 2 as viewed from above. As shown in FIG. 3, the filter array 205 includes a plurality of interference filters 301 to 306 having different transmission wavelength bands, arranged on the same circumference around the rotation axis 210. In this embodiment, interference filters having transmission wavelength bands of 420 nm, 520 nm, 570 nm, 610 nm, 710 nm, and 720 nm are used as the interference filters 301 to 306, respectively.

In the light emitting unit 101, a plurality of light sources 201a to 201c emit light in response to a signal from the control unit 111. Light emitted from the light sources 201a to 201c is directed by collimating lenses 202a to 202c corresponding to the light sources 201a to 201c, and their optical paths are combined by

dichroic mirrors

203a and 203b. Then, the beam diameter is adjusted by the aperture 204 and guided to the filter array 205. The filter array 205 is controlled to rotate around a rotation axis 210 parallel to the traveling direction of light in synchronization with the control of the plurality of light sources 201a to 201c, and has a specific wavelength from the light that has passed through the aperture 204 Select only transparent. The light transmitted through the filter array 205 is emitted from the light emitting unit 101 as light 300.

The half mirror 108 branches the light 300 emitted from the light emitting unit 101 into the chip 102 side and the reference light receiving unit 104 side.

The reference light receiving unit 104 receives the light branched by the half mirror 108 and supplies a signal based on the received light to the measuring unit 109.

FIG. 4 is a schematic view showing the chip 102, FIG. 4A is a front view of the chip 102 as viewed from above, and FIG. 4B is an example of the shape of the cell 400 provided in the chip 102. FIG. 4C is a schematic diagram showing the shape of the cell 410 provided in the chip 102.

As shown in FIG. 4A, the chip 102 has a disk shape, and a plurality of cells 400 and cells 410 are formed radially around the rotation shaft 450. In the present embodiment, six cells 400 and one cell 410 are formed on the chip 102. The

cells

400 and 410 are formed at equal intervals with respect to the circumferential direction of the chip 102.

The chip 102 is preferably made of, for example, a transparent material such as silicone, glass, or plastic so as to transmit the light 300 emitted from the light emitting unit 101 and passed through the half mirror 108. The chip 102 is more preferably made of a highly transparent synthetic resin in order to make the chip 102 inexpensive, and in this embodiment, the chip 102 is a low-density polypropylene that also has chemical resistance. It is made with.

The cell 400 and the cell 410 are not formed exposed on the surface of the chip 102, but are shown by solid lines so that the internal structure can be easily understood.

As shown in FIG. 4B, in each cell 400, a sample chamber 401 into which a soil extract (sample) extracted from soil is injected,

reagent chambers

402 and 403 for storing reagents, and a measurement chamber 404 are stored. And are formed. A flow path 405 is formed between the reagent chamber 403, the sample chamber 401, the reagent chamber 402, and the measurement chamber 404. The reagent chamber 403, the sample chamber 401, the reagent chamber 402, and the measurement chamber 404 communicate with each other. ing.

As shown in FIG. 4C, the cell 410 does not include a reagent chamber, and a sample chamber 411 and a reference chamber 414 are formed. Further, a flow path 415 is formed between the sample chamber 411 and the reference chamber 414, and the sample chamber 411 and the reference chamber 414 communicate with each other.

Further, the measurement chamber 404 of the cell 400 and the reference chamber 414 of the cell 410 are formed on the same circumference as indicated by a one-dot chain line A in FIG.

The size of the chip 102 is, for example, about 20 cm in diameter, and the size of each of the cell 400 and the cell 410 is 4 to 5 cm in the longitudinal direction in FIGS. 4B and 4C and 2 in the short direction. The size is about 3 cm.

The light receiving unit 103 receives the light 300 emitted from the light emitting unit 101 and transmitted through the region on the same circumference indicated by the alternate long and short dash line A in FIG.

The measuring unit 109 is connected to the reference light receiving unit 104 and the light receiving unit 103. The measuring unit 109 measures the intensity of the light received by each of the reference light receiving unit 104 and the light receiving unit 103 and calculates various data (soil component concentration, pH, etc.) based on the measurement result.

The rotation driving unit 106 is provided below the chip 102 and drives the chip 102 to rotate. In this embodiment, a stepping motor capable of pulse control is used as the rotation drive unit 106. In the present embodiment, the configuration in which the rotation drive unit 106 drives the chip 102 to rotate is shown, but the present invention is not limited to this, and the chip 102, the light emitting unit 101, and the light receiving unit 103 move relatively. do it. For example, the rotation driving unit 106 may be configured to rotationally drive the light emitting unit 101 and the light receiving unit 103 around the rotation axis 450, or to rotationally drive both the chip 102 and the light emitting unit 101 and the light receiving unit 103. It may be configured to.

The control unit 111 is connected to the light emitting unit 101, the measuring unit 109, and the rotation driving unit 106, and controls the operation of each unit.

Next, the operation of each part during measurement will be described.

5A shows a top view of the chip 102 at the time of measurement. FIG. 5B schematically shows a cross-sectional view taken along the line BB of the chip 102 in FIG. 5A, and the filter array 205 and the light receiving unit 103.

Hereinafter, when distinguishing each of the six cells 400 for convenience of explanation, reference numerals 400-a to 400-f are given, and when not distinguished, 400 reference signs are given. Similarly, when the sample chamber 401, the reagent chamber 402, the reagent chamber 403, the measurement chamber 404, and the flow path 405 formed in the cell 400 are distinguished from each other, 401-a to 401-f, 402-a, respectively. Reference numerals of .about.402-f, 403-a to 403-f, 404-a to 404-f, and 405-a to 405-f are attached.

0.4 ml of 5 wt% salicylic acid-sulfuric acid aqueous solution is injected into the reagent chamber 402-a of the cell 400-a, and 10 ml of 2 mol / l sodium hydroxide (NaOH) aqueous solution is injected into the reagent chamber 403-a in advance. . Similarly, the reagent is previously injected into the reagent chambers 402-b to 402-f and the reagent chambers 403-b to 403-f. Table 1 shows the types and amounts of the reagents previously injected into each of the reagent chambers 402-a to 402-f and the reagent chambers 403-a to 403-f. The column indicated by “−” in Table 1 indicates that no reagent has been injected.

As shown in Table 1, different reagents are injected into the reagent chamber 402 and the reagent chamber 403 of the cell 400, respectively. This is because the sample according to the soil component to be measured is injected, nitrate nitrogen (NO ₃ -N) is used in the cell 400-a, and ammonia nitrogen (NH ₄ -N) is used in the cell 400-b. In the cell 400-c, absorbable phosphoric acid (P ₂ O ₅ ), in the cell 400-d exchangeable potassium (K ₂ O), in the cell 400-e exchangeable calcium (CaO), In -f, exchangeable magnesium (MgO) is used as the soil component to be measured.

As a soil extract, 100 ml of water was added to 0.4 g of soil, shaken, filtered, and the filtrate was poured into the sample chamber 401 and the sample chamber 411. In addition, an open / close valve is provided in the flow path 405 between the reagent chamber 403 and the measurement chamber 404, and the valve is in a closed state.

When the measurement is started after injecting the soil extract into the sample chamber 401 and the sample chamber 411, the rotation driving unit 106 is first rotated. The rotation of the rotation drive unit 106 causes the chip 102 to rotate about the rotation axis 450, and the

cells

400 and 410 are respectively moved in the directions indicated by the arrows F in FIG. 4B and FIG. 4C. Centrifugal force (inertial force) acts.

The soil extract injected into the sample chamber 411 of the cell 410 moves to the reference chamber 414 through the flow path 415 by centrifugal force.

Further, the soil extract in the sample chamber 401 of the cell 400 and the reagent in the reagent chamber 402 move to the reagent chamber 403 through the flow path 405. As a result, a mixed liquid (measurement liquid) of the sample to be measured and the reagent is generated in the reagent chamber 403.

Next, the liquid mixture generated in the reagent chamber 403 of the cell 400 is stirred by the rotation of the rotation driving unit 106. In this embodiment, the stirring of the mixed liquid is performed by the rotation of the rotation driving unit 106, but is not limited thereto. The sample analyzer 100 may include a translation drive unit (not shown) that can be driven in one axis, and may be configured to perform stirring by a reciprocating motion of the translation drive unit. Alternatively, the rotation drive unit 106 and the translation drive unit may be used. It may be a configuration in which stirring is performed by driving in combination. Moreover, the structure which stirs by driving each of the rotation drive part 106 and the translation drive part with a time difference separately may be sufficient. Furthermore, the structure may be such that the rotation drive unit 106 rotates at a constant speed during agitation, a structure that rotates with acceleration, or a structure that rotates in reverse, or a combination of these. May be configured to rotate.

When the stirring of the mixed solution is completed, the open / close valve provided in the flow path 405 between the reagent chamber 403 and the measurement chamber 404 is opened, and the rotation driving unit 106 is rotated again, so that the mixed solution is mixed by centrifugal force. Is moved to the measurement chamber 404.

In addition, although stirring of the above-mentioned liquid mixture may be performed in the reagent chamber 403, it may be performed in the measurement chamber 404. Further, in the present embodiment, the mixed liquid is generated and stirred in the cells 400-a to 400-f by the rotation of the rotation driving unit 106. However, the present invention is not limited to this. Instead, it may be configured to be performed individually for each of the cells 400-a to 400-f. However, from the viewpoint of shortening the processing time, it is preferable that the cell 400-a to 400-f be configured to be performed collectively.

As shown in FIGS. 5A and 5B, when the stirring of the mixed solution is completed, the rotation of the rotation driving unit 106 causes the chip 102 to rotate at one uniform speed and the light from the light emitting unit 101 to the light 300. Is emitted, the light 300 scans the chip 102, and the light 300 transmitted through the chip 102 is incident on the light receiving unit 103.

Here, the light 300 emitted from the light emitting unit 101 is branched by the half mirror 108 into the chip 102 side and the reference light receiving unit 104 side. The measurement unit 109 measures and compares the intensity of the light incident on the reference light receiving unit 104 and the intensity of the light transmitted through the measurement chamber 404 and incident on the light receiving unit 103, thereby comparing the absorbance of the mixed liquid. (Transmittance) is calculated. Further, since the intensity of light before being incident on the chip 102 is measured by the reference light receiving unit 104, even if the output from the light emitting unit 101 fluctuates due to the influence of heat, light source deterioration, or the like, the mixture liquid can be transmitted. It is possible to accurately determine the rate.

FIG. 6 shows the position in the circumferential direction of the chip 102 and the intensity (transmission) of the light received by the light receiving unit 103 when the measurement is performed by rotating the chip 102 at a constant speed and scanning with the light 300 described above. It is a graph which shows the relationship with light quantity.

As shown in FIG. 6, when the light 300 passes through the measurement chambers 404-a to 404-f and the reference chamber 414, the amount of transmitted light increases. In addition, the mixed solution stored in the measurement chambers 404-a to 404-f exhibits a color reaction due to the reagent, and light absorption depending on the concentration of each soil component occurs. On the other hand, since only the soil extract is stored in the reference chamber 414, less light absorption occurs compared to the measurement chambers 404-a to 404-f. Therefore, in FIG. 6, it can be seen that the position where the amount of transmitted light is highest corresponds to the reference chamber 414.

Thus, by providing the cell 410 in which only the soil extract is stored in the chip 102, the measuring unit 109 rotates the chip 102 at a uniform speed around the circumference and scans it with the light 300, thereby receiving light from the light receiving unit 103. The position of the reference chamber 414 can be specified from the intensity of the light.

As shown in FIG. 6, when the light 300 passes through a place other than the measurement chambers 404-a to 404-f and the reference chamber 414, the amount of transmitted light shows the lowest value. This is because the chip 102 exhibits high absorbance due to the thickness of the structure constituting the chip 102.

However, when the light 300 transmitted through other than the measurement chambers 404-a to 404-f and the reference chamber 414 is incident on the light receiving unit 103, the measurement unit 109 sets the wrong position to the measurement chambers 404-a to 404-f. In addition, the position of the reference chamber 414 may be specified. Therefore, the measurement unit 109 can more clearly determine whether the light 300 is incident on the measurement chambers 404-a to 404-f and the reference chamber 414 or is incident on other locations. It is preferable.

Specifically, as shown in FIG. 7, in the portion of the chip 102 where the light 300 is scanned (transmitted), locations other than the measurement chambers 404-a to 404-f and the reference chamber 414 are previously shaded 460 ( It may be light-shielded (coated) by a light-absorbing material. Further, the structure of the filter array 205 shown in FIG. 3 may be made of a material having high light absorption.

Here, as described above, the measurement chambers 404-a to 404-f of the cells 400-a to 400-f and the reference chamber 414 of the cell 410 are formed at equal intervals on the same circumference. Therefore, the measurement unit 109 first identifies the position of the reference chamber 414 and determines the position of the identified reference chamber 414 as the origin, so that the position of a predetermined angle from the origin can be determined based on the relative positional relationship. Assuming that the positions are 404-a to 404-f, the positions of the measurement chambers 404-a to 404-f can be automatically determined.

When the measurement unit 109 specifies the positions of the reference chamber 414 and the measurement chambers 404-a to 404-f by the above-described method, the rotation drive unit 106 then determines a predetermined pulse value based on the origin. The chip 102 is rotated, and the amount of light transmitted through each of the measurement chambers 404-a to 404-f is measured. Thus, by specifying the positions of the reference chamber 414 and the measurement chambers 404-a to 404-f, the sample analyzer 100 can smoothly measure the amount of transmitted light.

The mixed solution stored in the measurement chamber 404-a has an absorption band depending on nitrate nitrogen in the vicinity of the wavelength band of 410 nm to 420 nm. Therefore, the blue LED of the light source 201b of the light emitting unit 101 is caused to emit light, and the filter array 205 measures the amount of transmitted light using the interference filter 301 of 420 nm. At that time, similarly, the blue LED of the light source 201b is caused to emit light with respect to the soil extract stored in the reference chamber 414, and the amount of light transmitted through the interference filter 301 is measured.

Similarly, the other measurement chambers 404-b to 404-f are used to measure the amount of transmitted light using the interference filters 301 to 306 and the light sources 201a to 201c corresponding to the mixed liquids stored therein, respectively. The same measurement is performed on the soil extract stored in the chamber 414.

The measurement unit 109 compares the amount of light transmitted through the measurement chambers 404-a to 404-f with the amount of light transmitted through the reference chamber 414 via the corresponding interference filter, obtains a difference, and determines each measurement chamber 404-a. Calculate the absorbance of ~ 404-f.

As described above, the sample analyzer 100 according to the present embodiment transmits the transmitted light amount (reference transmitted light amount) transmitted through the reference chamber 414 and received by the light receiving unit 103 and the measurement chambers 404-a to 404-f, Since the measurement is performed based on the comparison with the transmitted light amount (measured transmitted light amount) received by the light receiving unit 103, the soil component concentration and the like can be measured with high accuracy.

In the present embodiment, the stepping motor capable of positioning by pulse control is used as the rotation driving unit 106, but the present invention is not limited to this.

For example, in order to specify the position of the reference chamber 414, the rotation time may be measured together with the amount of transmitted light when the chip 102 is rotated once at a constant speed. With this configuration, the position of the reference chamber 414 is specified, and the time required for moving from the reference chamber 414 to each of the measurement chambers 404-a to 404-f based on the relationship between the amount of transmitted light and the required rotation time. Can be requested. Therefore, by controlling the time during which the chip 102 is rotated, it is possible to measure soil components at the positions of the respective measurement chambers 404-a to 404-f. By configuring the sample analyzer 100 as described above, a brushless motor or the like can be used as the rotation drive unit 106, and the sample analyzer 100 can be configured at a lower cost.

Furthermore, as shown in FIG. 5B, in the present embodiment, an example in which the measurement chamber 404 and the flow path 405 are sealed is shown, but the present invention is not limited to this, and as necessary. In addition, an air hole may be formed in the measurement chamber 404, the flow path 405, or the like.

In this embodiment, the chip 102 in which one reference chamber 414 and six measurement chambers 404-a to 404-f are formed is used as the chip 102. However, the reference chamber 414 is included in the chip 102. A plurality of may be formed.

Here, depending on the soil component to be measured, it is almost impossible to extract with a neutral solution, so it is necessary to extract with an acidic solution. In that case, a reference measurement chamber containing a soil extract α obtained by extracting a soil component with a neutral solution and a reference measurement chamber containing a soil extract β obtained by extracting a soil component with an acidic solution are formed. . And the reagent according to the soil component of a measuring object is mixed with said 2 types of soil extract, and it measures.

As described above, when it is necessary to prepare different extracts according to the target soil component, the reference transmitted light amount is measured for each of the different extracts by using a chip in which a plurality of reference chambers are formed. be able to. Therefore, the soil component can be measured with higher accuracy.

In the above-described embodiment, an example in which the reference chamber 414 and the measurement chamber 404 are formed in the chip 102 has been described, but the present invention is not limited to this. The present invention is also applied to a configuration in which a reference chamber and a measurement chamber are formed in a container other than a chip as long as it has a function of storing a soil extract (sample solution) and a mixed solution.

Further, in the present embodiment, a configuration is shown in which the concentration of a component is measured by absorbance using a reagent showing a color reaction, but the present invention is not limited to this. For example, a configuration in which a fluorescent reagent is used as a reagent and the amount of components is measured by measuring the emission intensity with a fluorescence detector may be used.

Moreover, in this embodiment, although the example which uses the soil extract extracted from soil as a sample of a measuring object was shown, a measuring object is not restricted to this. For example, a part of a crop body such as fruits and vegetables is extracted, water is added, an extract is prepared, shaken and filtered, and used as a sample. By measuring the sample thus prepared in the same manner as described above, the amounts of various components contained in crops such as fruits and vegetables can be measured. Furthermore, the sample analyzer 100 according to the present embodiment can also be used as a water quality measuring device that measures a plurality of components at once.

(Contrast with conventional technology)
Conventionally, when the sample analyzer automatically performs measurement, as a method of automatically recognizing the positional relationship of the cells, the sample analyzer is equipped with a detection unit such as a marker and the sample analyzer detects it. A method of providing a mechanism, or (2) a method in which a direction in which an analysis device is attached to a sample analyzer is determined in advance and a position of a measurement cell is recognized by a pulse value of an encoder or the like provided in a drive mechanism of the sample analyzer. ing.

Here, in order for the producer himself to perform the measurement, in addition to simple measurement work, the sample analyzer is required to be inexpensive. However, if the method (1) or (2) described above is employed, the problem that the sample analyzer becomes expensive, and restrictions on the work of mounting the analysis device on the analyzer increase, thereby increasing the work load. There is a problem of doing so.

In contrast, in the sample analyzer 100 according to the present embodiment, the reference chamber 414 is provided in the chip 102, the chip 102 is scanned with the light 300, and the measurement unit 109 specifies the amount of light transmitted through the reference chamber 414. As a result, the relative positions of the reference chamber 414 and the measurement chamber 404 on the chip 102 are specified.

Therefore, as in the prior art, a detection unit such as a marker is provided on the chip 102, a detection mechanism is provided on the sample analyzer 100, or a direction in which the chip 102 is attached to the sample analyzer 100 is determined in advance. It is no longer necessary to recognize the position of the measurement chamber 404 with a pulse value of an encoder or the like provided in the rotation drive unit 106, the sample analyzer 100 can be configured at a low cost, and measurement can be performed easily. it can.

[Embodiment 2]
Another embodiment of the present invention will be described. For convenience of explanation, members having the same functions as those described in the embodiment are given the same reference numerals, and descriptions thereof are omitted.

The sample analyzer 100 according to the present embodiment includes a light emitting unit 101 'different from the sample analyzer according to the first embodiment.

FIG. 8 is a schematic configuration diagram of the light emitting unit 101 ′ according to the present embodiment. FIG. 9 is a schematic view showing a filter array 215 provided in the light emitting unit 101 ′, FIG. 9A is a front view of the filter array 215 viewed from above, and FIG. 9B is a filter array. It is the enlarged view which looked at the reference | standard chamber filter 317 with which 215 is equipped from the upper direction.

The light emitting unit 101 ′ has the same configuration as the light emitting unit 101 provided in the sample analyzer 100 according to the first embodiment, except that the light emitting unit 101 ′ includes a filter array 215 instead of the filter array 205.

As shown in FIG. 9A, the filter array 215 includes six interference filters (measurement chamber filter, transmission region, and other transmission region) 311 at equal intervals on the same circumference around the rotation axis 310. 316 and a reference chamber filter 317 are provided.

Interference filters 311 to 316 and reference chamber filter 317 correspond to measurement chambers 404-a to 404-f and reference chamber 414 formed in chip 102, respectively. Therefore, the filter array 215 causes the light 300 to pass through the respective interference filters 311 to 316 and the reference chamber filter 317 when the measurement chambers 404-a to 404-f and the reference chamber 414 are scanned by the light 300. The measurement chambers 404-a to 404-f and the reference chamber 414 are controlled to pass through.

Specifically, the interference filter 311 is in the measurement chamber 404-a, the interference filter 312 is in the measurement chamber 404-b, the interference filter 313 is in the measurement chamber 404-c, and the interference filter 314 is in the measurement chamber 404-d. The filter 315 corresponds to the measurement chamber 404-e, the interference filter 316 corresponds to the measurement chamber 404-f, and the reference chamber filter 317 corresponds to the reference chamber 414.

In the present embodiment, as the interference filter 311 and the interference filter 314, an interference filter having a central wavelength band of 420 nm, an interference filter 312, an interference filter having a central wavelength band of 610 nm, and an interference filter 313 having a central wavelength band of 720 nm are used. As the interference filter 315, an interference filter having a central wavelength band of 570 nm was used, and as the interference filter 316, an interference filter having a central wavelength band of 520 nm was used.

Further, as shown in FIG. 9B, the reference chamber filter 317 includes five interference filters (reference chamber transmission region, other reference chamber transmission region) 317a to 317e, and the five interference filters 317a to 317e are provided. It arrange | positions so that it may rank with the circumference direction of the same periphery.

In the five interference filters 317a to 317e, the interference filter 317a is the interference filter 311 and the interference filter 314, the interference filter 317b is the interference filter 312, the interference filter 317c is the interference filter 313, and the interference filter 317d is the interference filter 315. The interference filter 317e is an interference filter having the same center wavelength band as that of the interference filter 316.

In the sample analyzer 100 according to the second embodiment of the present invention, in the measurement, a mixed solution is first prepared and stirred in the same procedure as in the first embodiment, and then mixed. Transfer of liquid and soil extract is performed. And the position of the reference | standard chamber 414 is pinpointed by rotating the chip | tip 102 by the rotation drive part 106 1 round at equal speed, and scanning with the light 300.

In the sample analyzer 100 according to the present embodiment, the amount of transmitted light (reference transmitted light amount) transmitted through the reference chamber 414 via each of the five types of interference filters 317a to 317e included in the reference chamber filter 317 during the scanning. It is characterized by performing measurements.

In the present embodiment, the light emitting unit 101 includes a white LED as the light source 201a, a blue LED as the light source 201b, and a red LED as the light source 201c, as in the first embodiment. In addition, the controller 111 controls the light source 201a, the light source 201b, and the light source 201c to emit light sequentially when the light 300 is transmitted through the reference chamber 414. The light emission switching period of the light source 201a, the light source 201b, and the light source 201c is determined from the rotation speed of the chip 102 by the rotation driving unit 106 and the widths of the five types of interference filters 317a to 317e included in the reference chamber filter 317. It is preferable that the light source 201a, the light source 201b, and the light source 201c are all turned on when passing through the interference filters 317a to 317e. In other words, the lighting cycle is such that the amount of light transmitted through the reference chamber 414 through each of the five types of interference filters 317a to 317e can be measured for all three light sources 201a to 201c. Is preferred.

When the scanning is completed, the chip 102 is then rotated by a predetermined amount, and any one of the predetermined light sources 201a to 201c is used for each of the measurement chambers 404-a to 404-f. The amount of transmitted light (measured transmitted light amount) is measured.

Then, the measured amount of light transmitted through the measurement chambers 404-a to 404-f and the amount of light transmitted through the reference chamber 414 through the corresponding interference filter at the corresponding light source are compared, a difference is obtained, and each measurement is performed. The absorbance of the chambers 404-a to 404-f is calculated.

By configuring the sample analyzer 100 as described above, the amount of transmitted light of the soil extract stored in the reference chamber 414 can be measured at a time for each wavelength, and the measurement can be performed very quickly. be able to. Moreover, it becomes possible to measure a soil component density | concentration etc. with high precision by taking the difference of the transmitted light quantity with respect to a soil extract, and the transmitted light quantity with respect to a liquid mixture.

[Embodiment 3]
Still another embodiment of the present invention will be described. For convenience of explanation, members having the same functions as those described in the embodiment are given the same reference numerals, and descriptions thereof are omitted.

The sample analyzer 100 according to the third embodiment of the present invention differs from the sample analyzer according to the second embodiment described above in the operation of each unit during measurement.

Specifically, after the position of the reference chamber 414 is specified, the light amount transmitted through the reference chamber 414 through each of the five types of interference filters 317a to 317e of the reference chamber filter 317 is measured again. , For each of the light source 201b and the light source 201c.

The measurement is preferably performed while rotating the chip 102 at a rotation speed slower than the rotation speed of the constant speed rotation of the chip 102 performed when the position of the reference chamber 414 is specified.

The measurement may be performed continuously while rotating the chip 102 as described above, but once the rotation of the chip 102 is stopped at each of the five types of interference filters 317a to 317e of the reference chamber filter 317. The measurement may be performed by sequentially turning on the light source 201a, the light source 201b, and the light source 201c.

With such a configuration, the amount of transmitted light of the soil extract stored in the reference chamber 414 can be measured more accurately. As a result, each of the stored amounts in the measurement chambers 404-a to 404-f can be measured. It becomes possible to further improve the measurement accuracy of the component concentration of the mixed liquid.

[Embodiment 4]
Still another embodiment of the present invention will be described. For convenience of explanation, members having the same functions as those described in the embodiment are given the same reference numerals, and descriptions thereof are omitted.

The sample analyzer 100 according to the present embodiment differs from the sample analyzer 100 according to the second embodiment described above in the operation of each unit during measurement.

Specifically, in the present embodiment, the liquid mixture is re-stirred immediately before measuring the absorbance in the measurement chamber 404-e. This is because the absorbance (turbidity) of the mixed solution of the diethanolamine mixed solution stored in the reagent chamber 403-e of the cell 400-e and the soil extract changes with time from immediately after stirring. This is based on what the present inventor found that the absorbance returns to the value immediately after stirring.

FIG. 10 is a graph showing the relationship between the component concentration and the absorbance when the absorbance was measured in advance using a soil extract with a clear component concentration. The plot indicated by the diamonds in FIG. This is data obtained by measuring the absorbance of the liquid without re-stirring, and the plot shown by a rectangle in FIG. 10 shows that the prepared liquid mixture is re-stirred before measurement and the absorbance is measured. It is the data performed.

As shown in FIG. 10, the rhombus plots that were not re-stirred were greatly deviated from the approximate curve, and the correlation between the component concentration and the absorbance was low. On the other hand, a rectangular plot obtained by re-stirring before measurement showed a high correlation between the component concentration and the absorbance.

Hereinafter, the operation at the time of measurement of the sample analyzer 100 according to the present embodiment will be described.

In the sample analyzer 100 according to the present embodiment, first, similarly to the sample analyzer 100 according to the second embodiment, first, the rotation drive unit 106 rotates the chip 102 one round at a constant speed, and scanning with the light 300 is performed. By doing so, the position of the reference chamber 414 is specified. Next, the amount of light transmitted through the reference chamber 414 is measured through each of the five types of interference filters provided in the reference chamber filter 317.

Thereafter, the absorbance is measured for each of the mixed solutions stored in the respective measurement chambers 404-a to 404-f by using predetermined light sources 201a to 201c.

At that time, the mixture is re-stirred before measuring the absorbance in the measurement chamber 404-e. The re-stirring of the mixed liquid is performed by the rotary drive unit 106 reciprocating the tip 102 in the circumferential direction. As a result, the measurement accuracy of absorbance can be remarkably improved.

Furthermore, when measuring the absorbance in the measurement chamber 404-b of the cell 400-b, similarly, the result that the measurement accuracy of the absorbance was greatly improved by re-stirring immediately before the measurement was obtained.

In this way, in a solution in which the color development reaction or dispersion state of the mixed solution of the reagent and the soil extract becomes unstable with the lapse of time after mixing, by re-stirring the mixed solution immediately before measurement, Absorbance can be measured with high accuracy.

On the other hand, there are mixed liquids in which the color development reaction or dispersion state of the mixed liquid is stable over time after mixing. When measuring the absorbance of the measurement chamber 404 in which such mixed liquid is stored, It is not necessary to re-stir the mixture immediately before the measurement.

As described above, the sample analyzer 100 according to the present embodiment re-stirs the mixed solution immediately before measuring the mixed solution in which the color development reaction or dispersion state of the mixed solution becomes unstable with respect to the lapse of time after mixing. As a result, the measurement accuracy of the absorbance, that is, the component concentration can be improved.

Furthermore, re-stirring is not performed when measuring a mixed solution in which the color development reaction or the dispersion state is stable over time after mixing. By this. Measurement time can be greatly reduced.

[Summary]
In the sample analyzer 100 according to the aspect 1 of the present invention, the reference chamber 414 containing the sample and the

measurement chambers

404, 404a to 404f containing the measurement liquid (mixed liquid) based on the sample are identical around the rotation axis 450. A light emitting unit 101 that emits light 300 toward the container (chip 102) and is disposed at a position corresponding to the same circumference as the container (chip 102) formed on the circumference, and transmitted through the container (chip 102). The light-receiving unit 103 that receives the light 300, the rotation driving unit 106 that rotates at least one of the container (chip 102), the light-emitting unit 101, and the light-receiving unit 103 around the rotation axis 450, and the reference chamber 414 are transmitted. Based on the reference transmitted light amount of the light received by the light receiving unit 103 and the measured transmitted light amount of the light 300 transmitted through the

measurement chambers

404 and 404a to 404f and received by the light receiving unit 103 And a measurement unit 109 for analyzing components contained.

According to the above configuration, the reference chamber 414 containing the sample and the

measurement chambers

404, 404a to 404f containing the measurement liquid (mixed liquid) based on the sample are disposed on the same circumference around the rotation shaft 450. (Chip 102). Therefore, the measurement can be performed simply and efficiently by rotating the container (chip 102).

Further, based on the reference transmitted light amount of the light 300 transmitted through the reference chamber 414 and received by the light receiving unit 103 and the measured transmitted light amount of the light 300 transmitted through the

measurement chambers

404 and 404a to 404f and received by the light receiving unit 103. Analyze the components contained in the sample. Therefore, accurate measurement can be performed.

In the sample analyzer 100 according to the second aspect of the present invention, in the first aspect, the reference chamber 414 contains only the sample liquid (soil extract) of the sample, and the measurement liquid (mixed liquid) is included in the sample. Is a mixed solution of a reagent and a sample corresponding to.

According to said structure, the sample analyzer 100 which concerns on aspect 2 of this invention compares the reference | standard transmitted light amount which permeate | transmits a sample liquid (soil extract), and the measured transmitted light amount which permeate | transmits a measurement liquid (mixed liquid). And analyze. Therefore, even if the sample liquid is colored, the reference transmitted light amount of the sample liquid can be used as a baseline, and high-precision measurement is possible.

In the sample analyzer 100 according to the aspect 3 of the present invention, in the aspect 1, the measurement unit 109 continuously measures the transmitted light amount of the light 300 received by the light receiving unit 103 along the same circumference, and the measured transmission Based on the amount of light, at least one position on the same circumference of the reference chamber 414 and the

measurement chambers

404, 404a to 404f is specified.

According to the above configuration, the measuring unit 109 specifies at least one position on the same circumference of the reference chamber 414 and the

measurement chambers

404, 404a to 404f based on the measured transmitted light amount. Thereby, it becomes possible to measure efficiently.

In the sample analyzer 100 according to Aspect 4 of the present invention, in the Aspect 3, the measurement unit 109 has the same circle in the reference chamber 414 depending on the position on the same circumference where the transmitted light amount of the light 300 received by the light receiving unit 103 is the largest. Specify the position on the circumference.

According to the above configuration, the measurement unit 109 identifies the position on the same circumference of the reference chamber 414 based on the position on the same circumference where the amount of transmitted light is the largest, so that the sample analyzer 100 can store the container (chip 102). The position of the upper reference chamber 414 can be recognized. Thereby, the sample analyzer 100 can automatically perform the measurement.

The sample analyzer 100 according to the aspect 5 of the present invention corresponds to the part corresponding to the reference chamber 414 and the

measurement chambers

404 and 404a to 404f among the parts of the container (chip 102) on the same circumference in the aspect 1 described above. It further includes a light shielding unit that shields light from the light emitting unit 101 that is incident on the part other than the part.

According to the above configuration, the light transmitted through the portion of the container (chip 102) excluding the portion corresponding to the reference chamber 414 and the portions corresponding to the

measurement chambers

404, 404a to 404f can be blocked. The measurement unit 109 can clearly specify the reference transmitted light amount and the measured transmitted light amount.

In the sample analyzer 100 according to the sixth aspect of the present invention, in the fourth aspect, the

other measurement chambers

404, 404a to 404f in which the other measurement liquid (mixed liquid) based on the sample is stored in the container (chip 102) are rotated. The measurement unit 109 is formed on the same circumference around the shaft 450, and the measurement unit 109 measures the measurement chamber based on the specified position of the reference chamber 414 and the relative positional relationship of the

measurement chambers

404 and 404a to 404f with respect to the reference chamber 414. 404, 404a to 404f are located on the same circumference, and based on the position of the identified reference chamber 414 and the relative positional relationship of the

other measurement chambers

404, 404a to 404f with respect to the reference chamber 414, The positions on the same circumference of the

measurement chambers

404, 404a to 404f are specified.

According to the above configuration, the position of the reference chamber 414 is first specified, and the positions of the

measurement chambers

404, 404a to 404f are specified based on the relative positions from the specified position of the reference chamber 414. As a result, even when there are a plurality of

measurement chambers

404, 404a to 404f, by specifying the positions of the

measurement chambers

404, 404a to 404f based on the specified positions of the reference chamber 414, the

respective measurement chambers

404, 404a to 404f Even if the measured transmitted light amount in 404f is a close value, the positions of the

measurement chambers

404, 404a to 404f can be reliably specified.

In the sample analyzer 100 according to aspect 7 of the present invention, in the aspect 6, the light emitting unit 101 has a wavelength corresponding to the measurement liquid (mixed liquid) in the

measurement chambers

404 and 404a to 404f whose positions are specified by the measurement part 109. The light sources 201a to 201c that emit the light 300 and the light 300 having other wavelengths corresponding to other measurement liquids (mixed liquids) of the

other measurement chambers

404 and 404a to 404f whose positions are specified by the measurement unit 109 are emitted. The light sources 201a to 201c emit the light 300 having the wavelength toward the

measurement chambers

404 and 404a to 404f, and the other light sources 201a to 201c include the

other measurement chambers

404 and 404a. The light 300 having the other wavelength is emitted toward .about.404f.

According to the above configuration, when there are a plurality of measurement liquids (mixed liquids), it is possible to emit light 300 having wavelengths corresponding to the positions of the specified

measurement chambers

404 and 404a to 404f. Therefore, even if a plurality of measurement liquids (mixed liquids) are stored in the container (chip 102), the measurement can be automatically performed.

In the sample analyzer 100 according to Aspect 8 of the present invention, in the Aspect 7, the light emitting unit 101 ′ corresponds to one of the

measurement chambers

404, 404a to 404f (of the interference filters 311 to 316). ), Another measurement chamber filter corresponding to the other one of the

other measurement chambers

404, 404a to 404f (the other one of the interference filters 311 to 316), and a reference chamber 414 And a measurement chamber filter (one of the interference filters 311 to 316) emitted from one of the light sources 201a to 201c to emit light from the

measurement chambers

404 and 404a to 404f. It includes a transmission region that transmits light of a wavelength corresponding to one of the measurement liquids (mixed liquid), and other measurement chamber filters (out of the interference filters 311 to 316) The other one) emits light from the other one of the other light sources 201a to 201c and corresponds to another measurement liquid (mixed liquid) of the other one of the

other measurement chambers

404 and 404a to 404f. The reference chamber filter 317 emits light from one of the light sources 201a to 201c to measure one of the

measurement chambers

404 and 404a to 404f. The reference chamber transmission region (one of the interference filters 317a to 317e) that transmits light of the wavelength corresponding to the (mixed liquid) and the other one of the other light sources 201a to 201c are used to emit light. Other reference chamber transmission regions (other interference filters 317a to 317e) that transmit light of other wavelengths corresponding to the other measurement liquid (mixed liquid) of the other one of the

measurement chambers

404 and 404a to 404f. One) , (One of the interference filter 317a ~ 317e) reference chamber transmissive region and (another one of the interference filters 317a ~ 317e) other criteria chamber transmissive region and are arranged on the same circle.

According to the above configuration, the light emitting unit 101 ′ includes a measurement chamber filter (one of the interference filters 311 to 316), another measurement chamber filter (the other one of the interference filters 311 to 316), By having the reference chamber filter 317, the measured transmitted light quantity through the measurement chamber filter (one of the interference filters 311 to 316) and the corresponding reference chamber transmission region (interference filter) of the reference chamber filter 317 are obtained. Based on the reference transmitted light quantity via one of 317a to 317e), the components contained in the sample can be analyzed for each

measurement chamber

404, 404a to 404f. Further, the reference chamber transmission region (one of the interference filters 317a to 317e) and the other reference chamber transmission region (the other one of the interference filters 317a to 317e) are arranged on the same circle. Accordingly, it is possible to measure the reference transmitted light amount corresponding to each of the

measurement chambers

404 and 404a to 404f at a time. Thereby, analysis can be performed with high accuracy and efficiency.

In the sample analyzer 100 according to the ninth aspect of the present invention, the transmitted light amount of the light received by the light receiving unit 103 is continuously measured along the same circumference in the above-described aspect 8, and the same transmitted light amount is the same on the same circumference. When the measurement unit 109 specifies the position on the same circumference of the reference chamber 414 based on the position of the reference chamber 414, the rotation driving unit 106 rotates the container (chip 102) at the first rotation speed, and the light source 201a to 201c is rotated. Light having a wavelength corresponding to one measurement liquid (mixed liquid) in one of the

measurement chambers

404 and 404a to 404f is transmitted through the reference chamber transmission region (one of the interference filters 317a to 317e). Then, light of another wavelength corresponding to another measurement liquid (mixed liquid) of the

other measurement chambers

404 and 404a to 404f emitted from one of the other light sources 201a to 201c is emitted. Other reference rooms When passing through the (other one of the interference filters 317a ~ 317e) over the region, the rotation drive unit 106 rotates motion at a slower second rotational speed than the container (tip 102) first speed of rotation.

According to the above configuration, by measuring the reference transmitted light amount while rotating the container (chip 102) at a slower speed than when the measurement unit 109 specifies the position on the same circumference of the reference chamber 414, The reference transmitted light amount can be accurately measured. This makes it possible to analyze the components of the sample with higher accuracy.

In the sample analyzer 100 according to the tenth aspect of the present invention, the transmitted light amount of the light received by the light receiving unit 103 while the rotation driving unit 106 rotates the container (chip 102) at the first rotation speed in the eighth mode. The measurement unit 109 continuously measures along the same circumference, the measurement unit 109 specifies the position on the same circumference of the reference chamber 414 based on the position on the same circumference where the transmitted light amount is the largest, and the light emitting unit 101 ′. The rotation drive unit 106 rotates the container (chip 102) around a predetermined angle rotation axis 450 from a position where the light emitted by the light passes through the reference chamber 414, and one of the

measurement chambers

404, 404a to 404f. In order to stir two measurement liquids (mixed liquids), the container (chip 102) is swung around the rotation shaft 450, and then emitted from the light emitting unit 101 ′ to be emitted from the

measurement chambers

404, 404a to 404f. Through the Chino one, the measurement amount of light transmitted through the light receiving unit 103 has received the measurement unit 109 measures.

According to the above configuration, the measurement liquid is obtained by peristating the container (chip 102) around the rotation axis 450 when performing measurement on one of the

predetermined measurement chambers

404, 404a to 404f. Re-stir (mixture). Therefore, even when measuring the measurement liquid (mixture) in which the color development reaction or dispersion state of the measurement liquid (mixture) becomes unstable over time after mixing, by re-stirring, The measurement accuracy of the component concentration can be improved.

The present invention is not limited to the above-described embodiments, and various modifications are possible within the scope shown in the claims, and embodiments obtained by appropriately combining technical means disclosed in different embodiments. Is also included in the technical scope of the present invention. Furthermore, a new technical feature can be formed by combining the technical means disclosed in each embodiment.

The present invention can be used for a sample analyzer, particularly a sample analyzer suitable for analyzing soil components.

100

Sample analyzer

101, 101 ′ Light emitting unit 102 Chip (container)
DESCRIPTION OF SYMBOLS 103 Light receiving part 106 Rotation drive part 109 Measuring part 201a-

201c Light source

205, 215 Filter array 300 Light 301-306 Interference filter 311-316 Interference filter (measurement room filter, transmission area, other transmission area)
317 Reference room filter 317a to 317e Interference filter (reference room transmission area, other reference room transmission area)
404, 404a to 404f Measurement chamber 414 Reference chamber 450 Rotating shaft

Claims

A container in which a reference chamber containing a sample and a measurement chamber containing a measurement liquid based on the sample are formed on the same circumference around a rotation axis;
A light emitting unit that emits light toward the container and is disposed at a position corresponding to the same circumference;
A light receiving portion for receiving light transmitted through the container;
A rotation driving unit that rotates at least one of the container, the light emitting unit, and the light receiving unit around the rotation axis;
Analyzing components contained in the sample based on a reference transmitted light amount of light transmitted through the reference chamber and received by the light receiving unit and a measured transmitted light amount of light transmitted through the measurement chamber and received by the light receiving unit. A sample analyzer comprising a measurement unit.
The reference chamber contains only the sample liquid of the sample,
The sample analyzer according to claim 1, wherein the measurement liquid is a mixed liquid of a reagent corresponding to a component contained in the sample and the sample.
The measurement unit continuously measures the amount of transmitted light received by the light receiving unit along the same circumference, and based on the measured amount of transmitted light, at least one of the reference chamber and the measurement chamber The sample analyzer according to claim 1, wherein a position on the same circumference is specified.
4. The sample analyzer according to claim 3, wherein the measurement unit specifies the position on the same circumference of the reference chamber based on the position on the same circumference where the amount of transmitted light received by the light receiving unit is the largest.
The light shielding part which shields the light from the said light emission part which injects into the site | part except the site | part corresponding to the said reference | standard chamber and the site | part corresponding to the said measurement chamber among the site | parts of the container on the said same periphery is further provided. The sample analyzer described in 1.
In the container, another measurement chamber containing another measurement liquid based on the sample is formed on the same circumference around the rotation axis,
The measurement unit identifies the position of the measurement chamber on the same circumference based on the position of the identified reference chamber and the relative positional relationship of the measurement chamber with respect to the reference chamber, and the identified reference The sample analyzer according to claim 4, wherein a position of the other measurement chamber on the same circumference is specified based on a position of the chamber and a relative positional relationship of the other measurement chamber with respect to the reference chamber.
The light emitting unit includes a light source that emits light having a wavelength corresponding to the measurement liquid in the measurement chamber whose position is specified by the measurement unit, and another measurement liquid in another measurement chamber whose position is specified by the measurement unit. Other light sources that emit corresponding wavelengths of light,
The sample analyzer according to claim 6, wherein the light source emits light of the wavelength toward the measurement chamber, and the other light source emits light of the other wavelength toward the other measurement chamber.
The light emitting unit, a measurement chamber filter corresponding to the measurement chamber;
Another measurement chamber filter corresponding to the other measurement chamber;
A reference chamber filter corresponding to the reference chamber;
The measurement chamber filter includes a transmission region that transmits light having a wavelength corresponding to the measurement liquid in the measurement chamber emitted from the light source;
The other measurement chamber filter includes another transmission region that emits light from the other light source and transmits light of another wavelength corresponding to the other measurement liquid of the other measurement chamber,
The reference chamber filter emits light from the light source and transmits light having a wavelength corresponding to the measurement liquid in the measurement chamber, and another measurement of the other measurement chamber emitted from the other light source. Another reference chamber transmission region that transmits light of another wavelength corresponding to the liquid,
The sample analyzer according to claim 7, wherein the reference chamber transmission region and the other reference chamber transmission region are arranged on the same circle.
The transmitted light quantity of the light received by the light receiving unit is continuously measured along the same circumference, and the position of the reference chamber on the same circumference is determined by the position on the same circumference where the transmitted light quantity is the largest. When the measurement unit specifies, the rotation drive unit rotates the container at a first rotation speed,
Light emitted from the light source and having a wavelength corresponding to the measurement liquid in the measurement chamber passes through the reference chamber transmission region, and is emitted from the other light source and corresponds to another measurement liquid in the other measurement chamber. 9. The sample analysis according to claim 8, wherein when the light having the wavelength of the second light passes through the transmission region of the other reference chamber, the rotation driving unit rotates the container at a second rotation speed lower than the first rotation speed. apparatus.
While the rotation drive unit rotates the container at the first rotation speed, the measurement unit continuously measures the transmitted light amount of the light received by the light receiving unit along the same circumference, and the transmitted light amount is The measurement unit identifies the position on the same circumference of the reference chamber by the largest position on the same circumference,
In order to stir the measurement liquid in the measurement chamber by rotating the container around the rotation axis by a predetermined angle from the position where the light emitted from the light-emitting unit passes through the reference chamber. The measurement unit measures the measured transmitted light amount of the light emitted from the light emitting unit and transmitted through the measurement chamber after the container is swung around the rotation axis and received by the light receiving unit. The sample analyzer described.