WO2016028061A1

WO2016028061A1 - Method for preparing a collecting plate, and an apparatus for measuring floating organisms

Info

Publication number: WO2016028061A1
Application number: PCT/KR2015/008618
Authority: WO
Inventors: 박철우; 이성화; 정춘수
Original assignee: 주식회사 엘지전자
Priority date: 2014-08-18
Filing date: 2015-08-18
Publication date: 2016-02-25
Also published as: CN106574222A; KR20160021973A; KR102288766B1

Abstract

The present invention relates to a method for preparing a collecting plate, and an apparatus for measuring floating organisms. A method for preparing a collecting plate, according to an embodiment, comprises the steps of: preparing a filter for collecting or filtering microorganisms; preparing a solvent for a coating portion for coating the filter; mixing a surfactant into the solvent; preparing a coating portion by diluting the solvent with a bioluminescent material; and coating the filter with the prepared coating portion.

Description

Collection plate manufacturing method and airborne microbial measuring device

The present invention relates to a method for producing a collecting plate and an apparatus for measuring suspended microorganisms.

Recently, avian influenza, swine flu, etc. have been raised, the problem of air infection has emerged, and thus airborne microbial measurement is more important, and the biosensor market is growing rapidly accordingly.

Conventional methods for measuring airborne microorganisms include collecting microorganisms suspended in a sample gas on a solid or liquid surface suitable for propagation, incubating in a suitable temperature and humidity environment for a certain period of time, and then collecting microorganisms in colony water that appeared on the surface. There are a culture method for obtaining water and a dyeing method using a fluorescence microscope after dyeing.

Recently, ATP bioluminescence method uses the principle that ATP (adenosine triphosphate) and luciferin / luciferase react to make light, which is a series of ATP scavenging process, ATP extraction and emission measurement The process has been reduced to about 30 minutes, allowing for quick work.

However, in order to apply the ATP bioluminescence method, an ATP extractant is basically required, but when it is used in a gaseous floating microbial measurement system, it may adversely affect humans. In addition, the ATP extractant must be continuously supplied in order to be applied to an automatic system, but there is a problem in that a cost burden is required to continuously supply the ATP extractant which is currently commercially available.

As such, since a series of manual operations are required for continuously measuring a microorganism in order to measure suspended microorganisms, the development of a system for automatically measuring suspended microorganisms in the weather is limited. .

The present invention has been proposed to improve the above problems, and an object of the present invention is to provide a method for manufacturing a collecting plate and a floating microorganism measuring device for quickly measuring the floating microorganisms without having to go through a series of manual operations for light emission of the floating microorganisms. It is done.

According to an aspect of the present invention to achieve the above or another object, preparing a filter for collecting or filtering microorganisms; Preparing a solvent of a coating unit for coating the filter; Mixing a surfactant in the solvent; Preparing a coating part by diluting a bioluminescent material in the solvent; And it provides a method for producing a collecting plate comprising the step of coating the prepared coating to the filter.

The method may further include adding an antifoaming agent to the coating part in which the bioluminescent material is diluted.

In addition, the antifoam can be sterilized and composed at a concentration of 0.02%.

In addition, it may further comprise the step of mixing the enzyme stabilizer in the prepared coating.

In addition, enzyme stabilizers may include sucrase or fructose.

In addition, the enzyme stabilizer may be composed of a concentration of 0.1 ~ 0.5M.

The method may further include drying the filter after coating the coating part on the filter.

In addition, the method may further include sterilizing the filter before coating the coating part.

In addition, the solvent may be distilled water.

The solvent may include a solvent, and the solvent may include an extractant or a degradable buffer (Lysis-Buffer).

In addition, the coating unit may be coated with 0.01ml to 0.1ml per 1cm ² area on the filter.

In addition, the sterilization may be wet heat sterilization or autoclave (Autoclave).

In addition, the concentration of the surfactant in the coating portion may be 0.1%.

According to another aspect of the present invention to achieve the above or another object, the particle fractionation unit including a filter for collecting or filtering the suspended microorganism; A microbial dissolution unit for dissolving suspended microorganisms collected or filtered in the particle sorting unit to extract adenosine triphosphate (ATP); A light receiving element installed at one side of the particle sorting unit and extracting the concentration or contamination level of the floating microorganism by detecting light of the floating microorganism emitted from the particle sorting unit; A display unit which displays data detected by the light receiving element; And a flow generating unit installed at the other side of the particle fractionation unit to flow air, wherein the filter is coated with at least one material selected from the group consisting of a surfactant, a bioluminescent material, a foam inhibitor, and an enzyme stabilizer. Microbiological measuring apparatus is provided.

In the surfactant, Triton X-100 may be composed at a concentration of 0.1%.

In addition, the bioluminescent material may include luciferin and luciferase.

In addition, the enzyme stabilizer, sucrase or fructose may be composed of a concentration of 0.1 ~ 0.5M.

According to the proposed invention, it is possible to apply the light emitting material to the floating microorganisms without going through a separate manual operation, there is an advantage that the measurement process of the floating microorganisms is simplified.

In addition, there is an advantage that the light emitting material can be uniformly distributed in the filter by adding the surfactant to the coating portion.

In addition, by adding a foam inhibitor to the coating portion, there is an advantage that the film is formed on the filter by the surfactant.

In addition, by adding the enzyme stabilizer to the coating portion there is an advantage that the light emission duration of the suspended microorganisms is maintained for a long time, the emission value of the suspended microorganisms can be increased.

1 is a conceptual diagram showing a floating microbial measurement apparatus according to an embodiment of the present invention.

2 is a conceptual diagram illustrating various embodiments of the particle classification unit.

3 is a perspective view of a collecting plate according to an embodiment of the present invention.

4 is a cross-sectional view of the collecting plate according to an embodiment of the present invention.

5 is a flow chart of a collecting plate manufacturing method according to an embodiment of the present invention.

Figure 6 is a graph showing the degree of bioluminescence according to the concentration of the enzyme stabilizer according to an embodiment of the present invention.

7 is an enlarged cross-sectional view of a filter when only a bioluminescent material is coated on the filter;

8 is an enlarged cross-sectional view of a filter when a coating part is formed on a filter according to an embodiment of the present invention;

Hereinafter, embodiments of the present disclosure will be described in detail with reference to the accompanying drawings, and the same or similar components are denoted by the same reference numerals regardless of the reference numerals, and redundant description thereof will be omitted. The suffixes "module" and "unit" for components used in the following description are given or used in consideration of ease of specification, and do not have distinct meanings or roles from each other. In addition, in describing the embodiments disclosed herein, when it is determined that the detailed description of the related known technology may obscure the gist of the embodiments disclosed herein, the detailed description thereof will be omitted. In addition, the accompanying drawings are intended to facilitate understanding of the embodiments disclosed herein, but are not limited to the technical spirit disclosed herein by the accompanying drawings, all changes included in the spirit and scope of the present invention. It should be understood to include equivalents and substitutes.

In the present specification, for the convenience of description, the airborne microbial measurement apparatus using ATP light emission will be described as an example. However, the present invention is not limited thereto, and the collecting plate according to the embodiment of the present invention may be applied to various floating microbial measuring apparatuses for emitting microorganisms.

1 is a conceptual diagram illustrating an apparatus for measuring suspended microorganisms according to an embodiment of the present invention, and FIG. 2 is a conceptual diagram illustrating various embodiments of a particle sorting unit.

1 and 2, the airborne microbial measurement apparatus 1 according to an embodiment of the present invention, the particle classification unit 10 for collecting and emitting microorganisms, and is collected in the particle classification unit 10 The microbial dissolution unit 20 which dissolves the microorganisms and extracts ATP (adenosine triphosphate), DNA, RNA, etc. in the microorganism, and is disposed at one side of the particle classification unit 10 to emit light from the particle classification unit 10. The light receiving device 30 for detecting the concentration or contamination of the microorganisms by detecting the light of the microorganisms, the display unit 40 for displaying the data detected by the light receiving device 30, and the particle sorting unit 10 Is installed on the other side of the flow includes a flow generating portion 50 for flowing air.

Although the particle sorting unit 10 is shown in a flat plate shape on FIG. 1, it is mainly used to express the action between the particle sorting unit 10, the microorganism dissolving unit 20, and the light receiving device 30. In this regard, only components corresponding to the collecting plate 11 are conceptually illustrated, and the shape and structure of the particle classification unit 10 are not particularly limited, and the particle classification unit 10 will be described below. Applicable in various embodiments.

The particle fractionation unit 10 may be a solid collecting method or a liquid collecting method for collecting particles in air, such as an electrostatic pricipitator, an inertial impactor, a cyclone, a centrifuge, or the like. The dust collector or filter system which has a collecting plate or a collection space which can be collected by this is called.

The electrostatic precipitator generates a corona discharge when a negative voltage (or a positive voltage) is applied to the discharge electrode by DC high voltage, and the negative ions (or positive ions) generated in the gas It is a dust collecting device that uses an electrostatic principle that is charged with dust particles and is moved and collected by an electric force to a collecting electrode (collection plate) to which a positive voltage (or negative voltage) is applied.

FIG. 2 (a) shows an example of a wire to plate type that is most widely applied among various electrostatic precipitator structures. An electric field is formed between a charging wire and a collecting plate. As it passes between the line and the collecting plate, the charged particles are collected in the collecting plate.

An inertial collision device has a structure in which an impingement plate or a receiving tube (hereinafter referred to as a 'collecting plate') is provided under an acceleration nozzle (impaction nozzle).

Figure 2 (b) shows an example of such an inertial collision device, the air passing through the acceleration nozzle or jet (jet) is changed by the collecting plate 90 ° of the flow direction, among the particles contained in the air Particles having a certain mass or more impinge on the collecting plate and are collected by the inertia without completely changing the flow direction.

Cyclone is one of centrifugal force separators widely used for separating solid particles in a fluid or separating liquid droplets from a gas, and has various kinds and specifications, and FIG. 2 (c) shows an example of such a cyclone. It is shown.

The air containing the particles is tangentially introduced inside the circular cyclone, and then forms a swirling flow along the inner wall of the cylinder, which is maintained by the centrifugal force while continuing to the cone region below the cyclone. It is pushed toward the inner wall and separated from the flow, and the particle-free flow (air) rises from the bottom of the cone to the top and is discharged through the outlet, and the separated particles descend through the cone inner wall to form a dust hopper or the like ( (Collectively referred to as "collection plate").

Centrifuge is a device that applies continuous centrifugal force generated when rotating at high speed. Cyclones and centrifugal force separators can be used to separate particles contained in air to the outer wall of the rotating vessel by using a rotating container that rotates at high speed compared to cyclone. have.

The electrostatic precipitator has a low pressure loss, which is suitable for applying a large capacity or a high flow rate agent, and has a high dust collection efficiency even for fine particles of nano size (100 nm or less). On the contrary, the inertial collision device, the cyclone, etc. have a simple structure and thus have low cost and maintenance cost.

The solid collecting method is a method for collecting a substance to be measured on a solid by adsorption, reaction, or the like by sucking the sample air through a particle layer of a solid, and the like. It is applicable in the process of collecting on a collecting plate or a collecting space.

The liquid collection method is a method of collecting a substance to be measured in the liquid by dissolution, reaction, precipitation, suspension, etc. by passing the sample air through the liquid or contacting the surface of the liquid. Will be different.

Floating microorganisms in the air may be collected by applying a liquid collecting method for applying or accommodating liquid on the collecting plate or the collecting space of the particle sorting unit 10.

In addition, by using the particle fractionation unit 10, a sample collection method for collecting the material to be measured by passing the sample air through the filter medium, the sample air to contact the condensation, such as a cooling tube and condensed material to be measured After using the principle of cooling condensation collection method to collect, direct collection method to directly collect the collection bag, collection bottle, vacuum collection bottle, syringe, etc. without dissolving, reacting and adsorbing the sample air, Analytical diffusion collection methods can also be applied.

The microorganisms suspended in the air are collected in the particle fractionation section 10 while passing through the particle fractionation section 10, and on the particle fractionation section 10 where microorganisms are collected, an ATP reactive light emitting agent necessary for ATP bioluminescence is provided. A coated collecting plate 11 is provided.

Therefore, the collection plate, the filter agent, and the filter system described above are constituted by the collecting plate 11, so that the ATP bioluminescence is achieved when the microorganism passes or collects the collecting plate 11.

The microbial dissolution unit 20 dissolves the microorganisms collected in the particle fractionation unit 10 or flows to the particle fractionation unit 10 using ions, electromagnetic forces, antimicrobial substances, thermal energy, and a catalyst. ATP (adenosine triphosphate), DNA, RNA, etc. are the collective components that extract the device, where dissolving the microorganisms do not dissolve the microorganisms in liquid state, but to break down a single microorganism into a number of elements It means to extract the elements of.

When the microorganism dissolving unit 20 is configured as an ion generator, power consumption increases as the diameter of the discharge tip provided in the ion generator increases, and when the power consumption increases, ozone harmful to the human body as well as ions. It is preferable to apply an ozone-free ion generator that uses a carbon brush having a diameter of the discharge tip of 10 μm or less, because it may occur up to.

According to the ozone-free ion generator using a carbon brush having a discharge tip diameter of 10 μm or less, it has a low power consumption of 4 W or less, and ozone is generated at less than 0.01 ppm. Ozone management standards of 0.06 ppm or less under Article 27 (1) of the Industrial Safety and Health Act can be satisfactorily satisfied.

When the microbial dissolution unit 20 is configured as an ion generator, the microbial cell wall of the microorganism is damaged by repulsion between charged ions attached to the microorganisms, and ATP is extracted. As a result of the collision of electrons, ATP is extracted while damaging the cell wall of the microorganism.

The ATP extracted by the microbial dissolution unit 20 is exposed to the outside of the cell of the microorganism and at the same time reacts with the ATP reactive light emitting agent in the particle sorting unit 10 to generate light, and converts the light into electricity. The light receiving element 30, such as a photodiode (PD), an avalanche photodiode (APD), detects light generated by ATP bioluminescence and extracts a concentration or contamination level of a microorganism.

All organisms store the energy from the oxidation of organic matter in a compound called ATP, and then hydrolyze it as needed to exercise and maintain body temperature using the energy released at that time. It also causes bioluminescence.

A light receiving element is a device that measures the photon flux or optical power by converting the energy of photons absorbed into the device into a form that can measure the energy. The light receiving element has the advantages of operating wavelength sensitivity, fast response speed, and minimum noise. Near-infrared region (0.8-1.6

It is widely used as a device for detecting optical signals in optical fiber communication systems operating in m).

In particular, photoelectric detectors of light-receiving elements generate carriers such as electrons and holes in a material constituting the element by photons absorbed by the element, and by the flow of the carriers A device in which a measurable current is generated, ie based on photoeffects, is suitable for application in the present invention.

The wavelength of the electromagnetic wave, which is felt brightly by the human eye, ranges from about 380 nm to 780 nm. For monochromatic light, the wavelength is short to celadon 400 to 500 nm, blue 450 to 500 nm, green 500 to 570 nm, sulfur 570 to 590 nm, and orange 590 to At 610 nm and red 610 to 700 nm, the light receiving element 30 has a sensitivity capable of receiving a wavelength band of 400 nm or more and 700 nm or less.

In collecting the airborne microorganisms in the particle fractionation unit 10, a pneumatic pressure difference forcing the flow of one side of the particle fractionation unit 10 to the other side by using a flow generator 50 such as a blower and a pump. Create In detail, the microorganism dissolving unit 20 and the light receiving element 30 are installed at one side of the particle sorting unit 10, which is a path through which air flows to the particle sorting unit 10, and the flow generating unit ( 50 is installed on the other side of the particle fractionation unit 10.

The higher the concentration of microorganisms, the greater the amount of ATP extracted and the greater the intensity of light. The light receiving element 30 converts the received light into electrical signals such as voltage, current, and frequency, and outputs it. do. The microbial concentration calculation unit (not shown) data-formulates or formulates or compares data-formulated or formulated relations between the electrical signal output from the light receiving element 30 and the bioluminescence value according to the microbial concentration.

The light detected by the light receiving device 30 displays a concentration or contamination level of the microorganism in real time through the display unit 40 through a signal processing process that is modified or dataized through the microorganism concentration calculator.

The particle sorting unit 10 includes a collecting plate 11 for collecting microbial particles. Hereinafter, the structure to the said collecting plate 11 is demonstrated. ,

3 is a perspective view of a collecting plate according to an embodiment of the present invention, Figure 4 is a cross-sectional view of the collecting plate according to an embodiment of the present invention.

3 and 4, in one embodiment of the present invention, the collecting plate 11 includes a filter 12 for collecting suspended microorganisms, and a coating unit coated on the filter 12 to emit suspended microorganisms. (14) includes.

The coating part 14 is formed by coating a light emitting solution on the filter 12.

In the present specification, the filter 12 is a concept of collectively filtering paper and a collecting substrate for filtration and collecting to measure suspended microorganisms. Accordingly, the filter 12 may be configured in various forms such as a porous filter through which air can flow or a plate form for collecting suspended microorganisms.

The filter 12 is formed in a substantially plate shape. The filter 12 is a component included in the particle fractionation unit in the airborne microbial measurement apparatus, which will be described later, and the airborne microorganisms contained in the air are collected in the filter 12. The filter introduced into the suspended microbial measuring apparatus may be a plate having a diameter of about 15 mm, but is not limited thereto. The filter may be formed in various sizes and shapes.

One or both surfaces of the filter 12 is provided with a coating 14 for emitting floating microorganisms. That is, in order to measure the concentration of the suspended microorganisms, the coating portion 14 is understood as a configuration for emitting the suspended microorganisms collected in the filter 12.

Hereinafter, a process of coating the coating part 14 on the filter 12 will be described.

5 is a flowchart illustrating a method of manufacturing a collecting plate according to an embodiment of the present invention.

First, the disc available for the filter 12 is cut into a size usable for the floating microbial measuring apparatus (S100), and the sterilized filter 12 is sterilized to remove residual fine dust and bacteria ( S110). Sterilization of the filter 12 is performed to reduce errors in suspended microbial measurement.

As a sterilization method, wet heat sterilization using steam and an autoclave method using pressurized steam may be used. The moist heat sterilization is a thermal method using steam, and the autoclave method is a steam sterilization method using an internal pressure vessel or high temperature used to react at high temperature and high pressure.

However, the present invention is not limited thereto, and various sterilization methods may be used to remove fine dust and bacteria remaining in the filter.

After the filter 12 is prepared, a solvent for preparing the coating unit 14 is prepared (S200). The coating unit 14 uses a solvent as a solvent. The solubilizer includes an extractant, Lysis-Buffer or distilled water. The solvent is also sterilized in the same manner as the filter 12.

After the sterilization process, the surfactant is added to the solubilizer (S300). When only the bioluminescent material is added to the solubilizer without adding the surfactant, the bioluminescent material is not uniformly mixed in the solubilizer, which causes difficulty in bioluminescence. Therefore, in the present embodiment, the bioluminescent material may be uniformly mixed into the solubilizer by adding a surfactant to the solubilizer. For example, the surfactant may use TritonX-100.

The surfactant is added to the solubilizer so that the final concentration is approximately 0.1%.

After the surfactant is mixed with the solvent, the bioluminescent material is diluted (S400). For example, the bioluminescent material may be luciferin. Bioluminescence is a kind of photochemical reaction in which an organic compound is oxidized by the action of an enzyme, and the energy released by the body is in the form of light energy. Luciferin, a luminescent substance, combines with ATP to form a complex of luciferin-ATP. Produce two molecules. Luciferin is here reduced and is therefore represented as LH2. (LH2 + ATP LH2-AMP + 2H3PO4)

The LH2-AMP generated in the reaction is in an unstable energy state as it reacts with oxygen and oxidizes, and thus, the unstable oxidized product decomposes to generate light (hv) while generating oxidized luciferin and AMP. Where L is the oxidized luciferin and L-AMP * indicates the luciferin-AMP complex in an unstable energy state (LH2-AMP + 1/2 O2 L-AMP * + H2O) (L-AMP * → L + AMP + hv ( Light energy)).

Bioluminescent materials are enzymes that perform catalysis and include luciferases.

Because LH2-AMP is reacted with oxygen (1/2 O2) and oxidized by the catalysis of an enzyme called luciferase, bioluminescence occurs in the presence of luciferin, ATP, luciferase and oxygen. It is calculated that 1 photon is emitted from oxidation of.

Therefore, if the bioluminescent material is composed of luciferin, by the above-described process, suspended microorganisms can be quickly measured within 5 minutes, and maximum luminance can be measured within 3 minutes (180sec), From this the measurement time can be shortened to within 3 minutes.

The bioluminescent material is diluted in the solubilizer at a concentration of 400 mg / ml.

After diluting the bioluminescent material in the solubilizer, a foam inhibitor is added (S500). The foam inhibitor can be added in a sterile state at a concentration of approximately 0.02%. Since a surfactant was added to uniformly mix the bioluminescent material in the dissolving agent, a film may be formed on the surface of the dissolving agent due to the chemical property of the surfactant. As a result, since the coating part 14 may be unevenly distributed on the filter, the antifoaming agent may be added to prevent the film from being formed on the surface of the dissolving agent.

An enzyme stabilizer may be added to the coating part 14 (S510).

6 is a graph showing the degree of bioluminescence according to the concentration of the enzyme stabilizer according to an embodiment of the present invention.

In the graph of FIG. 6, the horizontal axis represents the emission duration, and the vertical axis represents the degree of emission.

Referring to FIG. 6, it can be seen that when the enzyme stabilizer is added to the coating unit 14, the bioluminescence reaction lasts longer. That is, the suspended floating microorganisms can be measured for a longer time. And, it can be seen that the bioluminescence value is also increased due to the appropriate amount of enzyme stabilizer.

Sucrose and fructose may be used as the enzyme stabilizer, and the enzyme stabilizer is added to the coating unit 14 at a concentration of about 0.1 to 0.5 mol (M).

When the manufacture of the filter 12 and the luminescent solution is completed, the luminescent solution prepared in the filter 12 prepared to form the coating portion 14 is dropped (S600). The coating unit 14 is formed to distribute 0.01 ~ 0.1ml per 1cm2 area in the filter 12. After dropping the luminescent solution, the collecting plate 11 coated with the luminescent solution is dried in an environment having a humidity of 10% or less and deposited on the luminescent solution the filter 12 (S700). Therefore, when the luminescent solution is coated on the filter 12, the retention period at room temperature can be extended.

7 is an enlarged cross-sectional view of the filter when only the bioluminescent material is coated on the filter, and FIG. 8 is an enlarged cross-sectional view of the filter when the coating unit is formed on the filter according to an embodiment of the present invention.

Referring to FIG. 7, when only the bioluminescent material (eg luciferin) is coated on the filter, the pressure loss is increased because a film formed by the bioluminescent material is formed on the filter. Therefore, the luminous efficiency of the light emitting material to the floating microorganisms is lowered.

Referring to FIG. 8, when the coating unit 14 according to the present embodiment is coated on a filter, it may be confirmed that porosity is ensured by the surfactant and the antifoaming agent. Accordingly, the pressure loss may be reduced and the luminous efficiency of the light emitting material to the suspended microorganisms may be increased.

According to the proposed invention, it is possible to apply the light emitting material to the floating microorganisms without going through a separate manual operation, there is an advantage that the process of measuring the floating microorganisms is simplified.

In addition, there is an advantage that the light emitting material is uniformly distributed in the filter by adding the surfactant to the coating portion.

In addition, by adding an enzyme stabilizer to the coating portion, the light emission duration of the floating microorganisms is maintained for a long time, and the light emission value of the floating microorganisms is increased.

Claims

Preparing a filter for collecting or filtering the microorganisms;

Preparing a solvent of a coating unit for coating the filter;

Mixing a surfactant in the solvent;

Preparing a coating part by diluting a bioluminescent material in the solvent; And

Method for producing a collecting plate comprising the step of coating the prepared coating on the filter.
The method of claim 1,

The method of manufacturing a collecting plate further comprises the step of adding a foam inhibitor to the coating diluted the bioluminescent material.
The method of claim 2,

The foam inhibitor is sterilized and composed of a concentration of 0.02%.
The method of claim 1,

Method for producing a collecting plate further comprises the step of mixing the enzyme stabilizer in the prepared coating.
The method of claim 4, wherein

The enzyme stabilizer contains sucralase or fructose.
The method of claim 4, wherein

Enzyme stabilizer is a production method of the collecting plate, characterized in that composed of a concentration of 0.1 ~ 0.5M
The method of claim 1,

After coating the coating on the filter, the method of manufacturing a collecting plate further comprising the step of drying the filter.
The method of claim 1,

The method of manufacturing a collecting plate further comprises the step of sterilizing the filter before coating the coating.
The method of claim 1,

The solvent is a method for producing a collecting plate, characterized in that the distilled water.
The method of claim 1,

The solvent includes a solvent,

The solubilizer includes an extractant or a degradable buffer (Lysis-Buffer).
The method of claim 1,

The coating unit is a manufacturing method of the collecting plate, characterized in that the coating is coated with 0.01ml to 0.1ml per 1cm 2 area.
The method of claim 8,

The sterilization is wet heat sterilization or autoclave (Autoclave) manufacturing method of the collecting plate, characterized in that.
The method of claim 1,

Method for producing a collecting plate, characterized in that the concentration of the surfactant in the coating portion is 0.1%.
A particle fractionation unit including a filter in which suspended microorganisms are collected or filtered;

A microbial dissolution unit for dissolving suspended microorganisms collected or filtered in the particle sorting unit to extract adenosine triphosphate (ATP);

A light receiving element installed at one side of the particle sorting unit and extracting the concentration or contamination level of the floating microorganism by detecting light of the floating microorganism emitted from the particle sorting unit;

A display unit which displays data detected by the light receiving element; And

It is installed on the other side of the particle fractionation unit includes a flow generating unit for flowing air,

The filter is suspended microorganisms, characterized in that the filter is coated with one or more substances selected from the group consisting of surfactants, bioluminescent materials, foam inhibitors and enzyme stabilizers.
The method of claim 14,

In the surfactant, Triton X-100 is composed of a concentration of 0.1% airborne microbial measurement apparatus.
The method of claim 14,

The bioluminescent material, a floating microbial measurement apparatus that includes luciferin and luciferase.
The method of claim 14,

Suspension or fructose in the enzyme stabilizer, suspended microbial measurement device, characterized in that composed of a concentration of 0.1 ~ 0.5M.