WO2016000192A1 - Bioréacteur comportant une source lumineuse incorporée et procédé de culture de micro-algues - Google Patents

Bioréacteur comportant une source lumineuse incorporée et procédé de culture de micro-algues Download PDF

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Publication number
WO2016000192A1
WO2016000192A1 PCT/CN2014/081312 CN2014081312W WO2016000192A1 WO 2016000192 A1 WO2016000192 A1 WO 2016000192A1 CN 2014081312 W CN2014081312 W CN 2014081312W WO 2016000192 A1 WO2016000192 A1 WO 2016000192A1
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Prior art keywords
light
microalgae
light source
built
reaction vessel
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PCT/CN2014/081312
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English (en)
Chinese (zh)
Inventor
张玟籍
陈辉
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上海希宏生物科技有限公司
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Priority to PCT/CN2014/081312 priority Critical patent/WO2016000192A1/fr
Publication of WO2016000192A1 publication Critical patent/WO2016000192A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C12N1/125—Unicellular algae isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/89—Algae ; Processes using algae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • the invention relates to the field of biotechnology, and particularly relates to a built-in light source bioreactor and a microalgae cultivation method. Background technique
  • Microalgae biodiesel is the future development direction of liquid biofuels. It has high energy conversion efficiency, and the yield per unit area can be several tens of times higher than that of ordinary terrestrial crops, so that factory farming can be realized.
  • the principle of microalgae oil production is to use microalgae photosynthesis to convert the carbon dioxide produced in the chemical production process into the microalgae's own biomass to fix the carbon element, and then induce the reaction to convert the microalgae's own carbon material into oil. Then, the oil or fat in the microalgae cells is transformed into the extracellular cells by physical or chemical methods, and then refined to produce biodiesel, which can convert the nutrients and carbon dioxide in the waste gas into biofuels through photosynthesis of algae. , protein. Today, with the sharp rise in oil prices and the growing shortage of food, the industry has broad prospects for development.
  • microalgae cultivation is mainly divided into two stages, namely the breeding stage and the oil production stage.
  • microalgae cultivation microalgae mainly absorb red-yellow light and blue-violet light (wavelength range 620-700nm and 410-470nm), while absorbing carbon, nitrogen, phosphorus and other elements to reproduce without producing oil;
  • nitrogen source is no longer used to maintain a small amount of phosphorus and continuously enters C0 2 to maintain light.
  • the microalgae is significantly rich in oil production due to carbon and nitrogen starvation, and the reproduction rate is significantly reduced.
  • microalgae aquaculture photoreactor In order to further increase the unit yield of microalgae biodiesel and achieve three-dimensional culture, a dedicated microalgae culture photoreactor must be used.
  • the microalgae aquaculture photoreactor in the prior art is directly cultured in the open air compared with the runway pool and the multi-stage pool, the production density and the yield per unit area are obviously improved, but since the light source of the microalgae growth and oil production is still natural light, The use of sunlight by microalgae is not sufficient, it is uncertain and uncontrollable, and it runs counter to the stable and continuous requirements of factory production.
  • the auxiliary farming functions such as nutritional supplement and carbon dioxide aeration are not automated, making it practical. Industrialization applications still have a long way to go.
  • the object of the present invention is to provide a built-in light source bioreactor which can optimize the source of light source, carbon dioxide, nutrition, temperature and flow rate according to different breeding requirements of different kinds of algae, thereby realizing the stability of ultra-high density seaweed culture. , controllable, to meet the needs of industrialization.
  • a built-in light source bioreactor comprising:
  • a reaction vessel a reaction vessel is provided with a cover plate and the reaction vessel contains a culture liquid for the growth of the microalgae; an inlet and outlet device, the inlet and outlet device is sealingly connected with the reaction vessel, and a valve and a switch are arranged on the inlet and outlet device;
  • a light-emitting device wherein the light-emitting device is disposed inside the reaction vessel and when the reactor is in operation, the light-emitting device is at least partially or completely immersed in the culture liquid, thereby providing light required for the growth of the microalgae in the reaction container, wherein the light-emitting device emits
  • the light intensity of the light is uniform or substantially uniform in the depth direction (Z-axis direction) of the reaction vessel;
  • a gas distributor for supplying a gas required for the growth of microalgae into the reaction vessel is provided.
  • the light intensity of the light emitted by the light-emitting device is substantially uniform in the horizontal direction of the reaction vessel (including the X-axis and Y-axis directions).
  • the "uniform or substantially uniform" means that the intensity D1 at any depth and the average intensity Dm over the entire depth range satisfy the following formula:
  • a temperature control device is provided around the reaction vessel for maintaining the liquid ambient temperature within the reaction vessel within a range suitable for the growth of the microalgae.
  • the reactor is further provided with a nutrient distribution device for providing the nutrients required for the growth of the microalgae in the reaction vessel.
  • the gas distributor is a rotary gas distributor, and during the passage of the gas into the reaction vessel, the rotary gas distributor rotates to promote the dispersion of the gas and nutrients in the liquid culture system.
  • the temperature control device is a temperature controlled water pipe.
  • the range suitable for the growth of the microalgae means 15-45 ° C, preferably 20-40 ° C.
  • the built-in light source bioreactor is further provided with a monitoring system for monitoring parameters of the liquid environment, the parameters being selected from the group consisting of: ra value, temperature and/or nutrient concentration.
  • the light emitting device includes a light guide plate and a light emitting unit, and the light generated by the light emitting unit is transmitted through the light guide plate to cause the light guide plate to emit light as a whole.
  • the light-emitting device further includes a bracket for fixing and supporting the light guide plate, the bracket being detachably coupled to the reaction container and/or the cover plate, and the light-emitting unit being embedded in the light guide plate.
  • an air vent is provided in the cover.
  • the lighting unit is an LED lighting unit.
  • the built-in light source bioreactor is provided with a plurality of light guide plates, preferably 3-100 pieces, more preferably 4-80 pieces, and most preferably 5-50 pieces.
  • the light guide plate is made of a transparent organic material having weak acid resistance.
  • the illumination device emits at least 2 different wavelengths of light when in operation.
  • the light of the different wavelengths includes: light having a wavelength of 600-800 (preferably 650-750) nm and light having a wavelength of 400-480 (preferably 430-470) nm.
  • the color temperature of the light emitted by the light emitting unit is 1000-20000K, preferably
  • the light-emitting unit is an LED, and the number of the LEDs is 1-10000 / light guide plate; preferably 10-1000 / light guide plate.
  • a production aquaculture apparatus comprising the built-in light source bioreactor of the first aspect of the invention.
  • At least two of the built-in light source bioreactors are connected in series and/or in parallel.
  • the interconnected reactors are connected by an inlet and outlet device.
  • the production aquaculture equipment is used as a production and breeding system for Chlorella, Chlorella, Cyanophyta and Red algae microalgae.
  • a method of cultivating comprising the steps of:
  • a method of preparing microalgae for producing a material diesel comprising the steps of:
  • the culture comprises a first culture stage and a second culture stage, in the first stage of the growth of the microalgae, the illumination
  • the light emitted by the unit has a wavelength of 350-900 nm, preferably 570-800 and 400-500 nm; in the second stage of microalgae growth, the light emitted by the light-emitting unit has a wavelength of 350-900 nm, preferably 600-800 and 400-480nm;
  • the illuminating unit in the first stage of microalgae growth, the illuminating unit emits a wavelength of 570-800 nm; in the second stage of microalgae growth, the illuminating unit emits at a wavelength of 400-480 nm.
  • a nitrogen source is provided to the built-in light source bioreactor during the first stage of microalgae growth; in the second stage of microalgae growth, the supply of nitrogen source to the built-in light source bioreactor is stopped.
  • the method further comprises: processing the recovered microalgae (eg, drying, breaking, extracting, transesterifying, etc.) to produce biomass diesel.
  • processing the recovered microalgae eg, drying, breaking, extracting, transesterifying, etc.
  • a microalgae which can be used for the production of a substance diesel which is prepared by the method described in the fourth aspect.
  • microalgae has the following characteristics:
  • the size of the microalgae is 5-500 microns
  • a method of producing biomass diesel characterized in that it comprises the steps of: using the microalgae according to the fifth aspect of the invention as a raw material, and processing, thereby producing biomass diesel oil.
  • the processing of the microalgae comprises: drying, extraction, extraction, transesterification, and the like.
  • FIG. 1 is a perspective cross-sectional view of a built-in light source bioreactor according to an embodiment of the present invention
  • FIG. 2 is a perspective cross-sectional view of a built-in light source bioreactor according to an embodiment of the present invention
  • FIG. 3 is a schematic view of a built-in light source bioreactor according to an embodiment of the present invention
  • 4 is a schematic cross-sectional view of a built-in light source bioreactor
  • FIG. 4 is a top cross-sectional view of a built-in light source bioreactor according to an embodiment of the present invention
  • FIG. 5 is a light-emitting of a built-in light source bioreactor according to an embodiment of the present invention.
  • a schematic view of the device
  • Figure 6a is a front elevational view of a light emitting device incorporating a light source bioreactor in accordance with one embodiment of the present invention
  • Figure 6b is a side view of a light emitting device with a built-in light source bioreactor, in accordance with one embodiment of the present invention
  • Figure 6c is a top plan view of a light emitting device with a built-in light source bioreactor according to one embodiment of the present invention
  • Figure 6d is an enlarged view of the portion B according to Figure 6b. detailed description
  • the inventors have extensively and intensively researched and developed a built-in light source bioreactor for the first time.
  • the reactor of the present invention not only shortens the culture time but also significantly increases the biomass density of the microalgae through a specially designed light-emitting device or the like. And the total effective fat content, so that better quality biodiesel can be prepared.
  • the present invention has been completed on this basis. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The preferred embodiments of the present invention will be described in detail with reference to the appended claims The embodiment shown in the drawings is not intended to limit the scope of the invention, but only to illustrate the spirit of the invention. Bioreactor
  • the built-in light source bioreactor includes a reaction vessel 1, a light-emitting device 2, an inlet and outlet device 3, a cover plate 5, a gas distributor 6, a nutrient distribution device, a temperature control device 7, and monitoring. And control systems, etc.
  • the reaction vessel 1 is provided with a cover plate 5, and the reaction vessel 1 is sealingly connected with the inlet and outlet device 3, and a valve and a switch are arranged on the inlet and outlet device for controlling the start or stop of the inlet and outlet, and the reaction vessel 1 is provided with a light-emitting device.
  • a temperature control device 7 (shown as a temperature control water pipe) is disposed around the reaction vessel for maintaining the temperature of the solution in the reaction vessel within a temperature range suitable for microalgae propagation and oil production.
  • the lower portion of the bioreactor (e.g., about 30% to 90%, or 50% to 70% of the height) can be buried underground.
  • the bioreactor can be installed around the power plant, and the waste water generated by the power generation can be used as a source of material and energy for microalgae cultivation, and has the function of environmental protection and emission reduction.
  • the reaction vessel 1 may be a closed container that is opaque to light, and may be illuminated by its own light-emitting device during rainy days and nights for the growth of microalgae.
  • a monitoring system is provided in the built-in light source bioreactor for monitoring the solution ra value and temperature in the reactor.
  • the built-in light source bioreactor of the present invention can also be used for silk algae, dinoflagellates or other aquatic organisms. High-density industrial farming.
  • FIG 3 is a front cross-sectional view of the built-in light source bioreactor; as shown in Figure 3, a temperature control device 7 is provided on the wall of the reaction vessel, and the temperature control device 7 can be in any suitable form, such as a temperature control compartment. set.
  • the temperature control device 7 is a temperature control water pipe, and the wastewater having a certain temperature discharged from the power plant can be used to circulate in the temperature control water pipe to keep the temperature in the bioreactor between 15 ° C and 45 ° C.
  • An air vent is provided at the top of the cover 5 of the bioreactor for releasing oxygen generated by photosynthesis of the microalgae.
  • FIG 4 is a top cross-sectional view of the built-in light source bioreactor.
  • a gas distributor 6 is provided at the bottom of the built-in light source bioreactor.
  • the gas distributor 6 can be in the form of a rotary, trough, tubular or any other suitable form.
  • carbon dioxide, air or other gas is introduced into the reaction vessel through a gas distributor.
  • a rotary gas distributor is used.
  • the gas distributor 6 rotates to drive the gas in the bottom container and the liquid in the bottom container, thereby facilitating the gas and nutrient in the solution. Evenly distributed.
  • a nutritional drape device is installed at one or more locations of the reaction vessel (e.g., on the vessel wall, at the bottom of the vessel, at the top of the vessel, and inside the cover, etc.).
  • the nutrient cloth device provides different nutrients at different stages of microalgae growth.
  • the reaction vessel into the rate of other nutrients and the flow speed of C0 2 may be such proportion, the amount of dissolution solution is C0 2 by the C0 2 online monitoring system may determine that, while the ra value obtained by the monitoring system monitoring the solution known solution The pH.
  • the control system can control the rate of introduction of co 2 and nutrients to ensure that the amount of nutrients dissolved in the solution and the ra value are within the range suitable for the growth or production of microalgae.
  • Fig. 5 is a perspective view of the light-emitting device 2.
  • the light-emitting device is composed of a bracket 9, an LED light-emitting unit group 10 and a light guide plate 11, the light guide plate 11 is fixed by a bracket 9, and the top end of the bracket 9 is fixed on the cover plate 5, and the LED light-emitting unit group 10 is located.
  • the light guide plate 11 or all of them protrude below the liquid surface.
  • a plurality of light-emitting devices 2 are provided, and each of the light guide plates can be separately mounted or detached.
  • the light guide plate can be made of a transparent organic material such as acrylic and has weak acid resistance.
  • the LED light emitting unit group is in direct contact with the light guide plate at the top of the light guide plate, and the light emitted by the LED light emitting unit group can transmit light.
  • the plate conducts and causes the light guide plate to emit light as a whole.
  • the top end of the bracket 9 of the illuminating device 2 is detachably connected to the cover 5 to facilitate removal, replacement or reinstallation of the illuminating device; the illuminating device 2 is suspended in the reaction container without contacting the bottom of the container, thus not affecting the gas at the bottom of the container The rotation of the spreader. It should be understood that the holder of the illumination device can also be secured to the reaction container at any suitable location in any suitable manner.
  • Figures 6a-6c are front, side and top views, respectively, of a light-emitting device with a built-in light source bioreactor;
  • Figure 6d is an enlarged view of a portion B of Figure 6b.
  • the LED lighting unit group 10 includes a small LED lighting unit.
  • the light generated by the light unit can be composite color or monochromatic light.
  • the color range of the monochrome LED is 350-900 nm, and the color temperature range of the composite light is 1500-20000K.
  • the number of LED lighting units on a single light guide plate can be from 1 to 10,000.
  • the light intensity of the light emitted by the light-emitting device is uniform or substantially uniform in the depth direction (Z-axis direction) of the reaction vessel; the light intensity of the light emitted by the light-emitting device is in the horizontal direction of the reaction vessel (including the X-axis and the Y-axis)
  • the axis direction is basically uniform.
  • "Uniform or substantially uniform” means the intensity D1 at any depth and the average intensity over the entire depth range. Dm satisfies the following formula:
  • the illuminating device is capable of emitting at least 2 different wavelengths of light when operating.
  • the different wavelengths of light include: light having a wavelength of 570-800 nm and light having a wavelength of 400-500 nm.
  • the LED lighting unit group 10 may be located at the bottom of the light guide plate 1 1 or at other suitable positions of the illuminating panel, as long as the light emitted by the LED light guide plate can be conducted in the entire light guide plate so that the entire light guide plate emits light.
  • the energy source of the LED lighting unit group is the electric energy generated by the solar panel absorbing solar energy.
  • the bioreactor of the present invention does not directly utilize solar energy, the unstable solar energy is collected by the solar panel for power generation, and the generated electricity is stably supplied to the light-emitting unit group of the bioreactor for continuous operation. Luminescence ensures the stability and sustainability of microalgae culture. Production culture system
  • microalgae The propagation and oil production stages of microalgae can be carried out in the same bioreactor, but it is necessary to change the type and rate of nutrient access as the growth progresses, and the process is complicated.
  • bioreactors can be used in series or in parallel to form large gauges. Mold production system.
  • the series or parallel connection between the bioreactors is connected through the inlet and outlet ports, and the feed and discharge between the bioreactors can be completed by the pump system.
  • a plurality of bioreactors can be used in series, for example, a system in which three bioreactors are connected in series to complete micro The entire growth process of algae reproduction and oil production:
  • a light-emitting device having an emission wavelength suitable only for facilitating the propagation of microalgae is provided, and the nutrient distribution device is supplied with ammonium phosphate, potassium dihydrogen phosphate or dipotassium hydrogen phosphate into the reaction container.
  • Nitrogen oxides are used as nitrogen and phosphorus sources, and elements such as iron and zinc are added at the same time.
  • the gas distributor introduces nitrogen oxides and CO 2 into the reaction vessel, and the luminous intensity and nutrient supply rate in the second-stage bioreactor are higher than those of the first-order organisms.
  • the reactor is large to accommodate the growth requirements of the microalgae with increased reproduction; in the third-stage bioreactor, the illuminating wavelength is only suitable for the light-emitting device which is beneficial to the production of microalgae, and the PH and temperature are simultaneously adjusted to be suitable for microalgae production. Under the condition of the oil, and the nutrient distribution device does not provide a nitrogen source, the gas distributor introduces C02 into the reaction vessel.
  • Different grades of bioreactors can be sized according to their needs. For example, the size of the first stage bioreactor is smaller than the latter two stages.
  • new algae species can be introduced into the first-stage bioreactor while a new round of culture is carried out.
  • a portion of the algae species as the first stage bioreactor can be filtered from the microalgae discharged from the second stage bioreactor.
  • the present invention provides a method of culturing microalgae, the method comprising the steps of:
  • the algae species are placed in the built-in light source bioreactor to provide the nutrients (including nitrogen sources, phosphorus sources, inorganic salts (such as iron, zinc), etc.) required for the survival of the microalgae and provide carbon dioxide or air. Turn on the illuminating device to generate the light needed for the growth of the microalgae.
  • nutrients including nitrogen sources, phosphorus sources, inorganic salts (such as iron, zinc), etc.
  • the present invention provides a method for culturing microalgae producing biomass diesel, the method comprising the steps of: 1. Providing a built-in light source bioreactor of the invention;
  • the algae species are placed in the built-in light source bioreactor to provide the nutrients (including nitrogen sources, phosphorus sources, inorganic salts (such as iron, zinc), etc.) required for the survival of the microalgae and provide carbon dioxide or air. Opening the illuminating device to generate light required for the growth of the microalgae, wherein
  • the light emitted by the light emitting unit has a wavelength of 350-900 nm, preferably
  • the light emitted by the light-emitting unit has a wavelength of 350-900 nm, preferably 600-800 and 400-480 nm.
  • the first stage and the second stage of the growth of the microalgae different wavelengths of light are used as the light source, and the supply of the nutrient and the gas is used to make the microalgae mainly propagate in the first stage, and the second stage mainly produces oil.
  • the first stage red light is used as the light source and the nitrogen source is passed, and in the second stage, blue light is used as the light source and the nitrogen source is stopped.
  • microorganism suitable for use in the present invention is not particularly limited as long as it can be grown using a light source.
  • Representative microorganisms include (but are not limited to): Chlorophyta, Cyanophyta, Chlorella, and Red algae microalgae.
  • a preferred microorganism is a freshwater species of Nannochlorops i s l imne t i ca.
  • the present invention provides a method for preparing biomass diesel using a microalgae prepared by the aforementioned method for producing biomass diesel as a raw material, thereby processing to produce biomass diesel, and a typical processing process includes Steps:
  • microalgae are collected by filtration, pressure filtration or bubble suspension, and then the algae oil and the algae are separated by cellulase hydrolysis and homogenization, and the pure algae oil is obtained by extraction, leaching or pressure filtration.
  • Biodiesel that can be directly used in diesel engines is obtained after transesterification, solvent dilution or thermal decomposition.
  • the present invention has the following main advantages:
  • the bioreactor in the prior art relies on external natural light, and in the case of weak weather such as rainy days, the microalgae cannot obtain sufficient light source to slow down growth, and the built-in light source bioreactor of the present invention is not The impact of weather changes;
  • the prior art is not suitable for large-scale aquaculture, because only a microalgae close to the upper surface of the pool can obtain a sufficient light source, and the bioreactor of the present invention has a plurality of uniformly distributed light-emitting devices built therein, and the present invention
  • the gas distributor and nutrient distribution device in the bioreactor of Mingming are beneficial to the uniform distribution of nutrients in the reaction vessel, so that large-scale aquaculture, three-dimensional aquaculture can be realized, and the breeding efficiency can be improved;
  • the bioreactor of the invention can adopt different built-in light sources and nutrition, temperature, pH and the like according to different growth stages of the microalgae, so that the microalgae propagation is carried out in stages, and the controllability is strong, which is favorable for achieving stable and continuous Factory production;
  • the series and/or parallel system of the bioreactor of the present invention makes the large-scale cultivation of microalgae more efficient.
  • Example 1 the built-in light source bioreactor of the present invention as shown in Fig. 1 was employed.
  • Example 1 the built-in light source bioreactor of the present invention as shown in Fig. 1 was employed.
  • the conventional Nannochlorops isl imnetica freshwater species was selected, the initial culture density was 0. lg/L, and the reactor liquid depth was 80 cm (the cross-sectional area was lm X 1.5 m).
  • a red LED with a wavelength of 706 nm is used as a light source, and the temperature is constant at 24 ° C.
  • a trace amount of ammonium phosphate, zinc, iron and other trace element fertilizers are preliminarily added to the culture solution, and a certain amount of C0 2 is introduced (about 90%).
  • nitrogen oxides such as NO or N0 2 as a nitrogen source are placed in the algae species at the initial culture density after one hour, and the culture is started.
  • the biomass density was increased to 6.86 g/L (dry weight/solution, the same below). After 72 hours, the biomass density was 9.71 g/L, and the microalgae in the reactor was moved to another reaction.
  • the device liquid surface depth 80cm, cross-sectional area 2m X 3m
  • stop the supply of nitrogen oxides keep C0 2 supply
  • use blue LED with wavelength of 450nm as the light source the temperature is constant at 28 °C
  • move to another reaction After 24 hours, the water sample was taken, the nitrogen source was almost exhausted, and the biomass density was 10.64 g/L. After 72 hours of moving to another reactor, the biomass density increased to 12.88 g/L. A total of 144 hours.
  • the distribution of microalgae in the reactor was visually observed. 3%, The total effective fat content is 40. 39%, the total lipid content (dry weight) is 45.08%, the triglyceride fatty acid ester (triglyceride) accounts for 89.6%, the total effective fat content is 40.39%. , total effective lipid content density of 5. 20g / L.
  • Example 2 The freshwater species of Nannochloropsis 1 imnetica was selected, the initial culture density was 0. lg/L, and the liquid level of the reactor was 80 cm (the cross-sectional area was ImX1.5 m). In the first stage, a blue LED with a wavelength of 450 nm is used as a light source, and the temperature is constant at 24 ° C.
  • a trace amount of ammonium phosphate, zinc, iron and other trace element fertilizers are preliminarily added to the culture solution, and a certain amount of C0 2 (about 90%) is respectively introduced.
  • nitrogen oxides such as NO or N0 2 as a nitrogen source are placed in the algae species at the initial culture density after one hour, and the culture is started.
  • the biomass density increased to 5.66 g/L after 36 hours, and the biomass density was 8.03 g/L after 72 hours.
  • the microalgae in the reactor was moved to another reactor (the depth of the liquid surface was 80 cm, and the cross-sectional area was In 2mX3m), the supply of nitrogen oxides is stopped, the supply of C0 2 is kept, and the red LED with a wavelength of 706 nm is used as the light source.
  • the temperature is constant at 30 ° C.
  • the water sample is taken, and the nitrogen source is basically After depletion, the biomass density was 9.21 g/L.
  • the biomass density increased to 10.52 g/L, and the culture ended, for a total of 144 hours.
  • the distribution of microalgae in the reactor was visually observed. After filtration, the microalgae were obtained.
  • the total lipid content (dry weight) was 43.98%
  • the triacylglycerol fatty acid ester (triglyceride) accounted for 77.7% of the total lipid content
  • the total effective fat content was 34.17%
  • the total effective fat content was The density is 3.59 g/L.
  • the fresh water species of Nannochloropsis 1 imnetica was selected, and the initial culture density was 0. lg/L, and the liquid level of the reactor was 80 cm (the cross-sectional area was 2 m ⁇ 3 m).
  • a red LED with a wavelength of 706 nm is used as a light source, and the temperature is constant at 24 ° C.
  • a trace amount of ammonium phosphate, zinc, iron and other trace element fertilizers are preliminarily added to the culture solution, and a certain amount of C0 2 is introduced (about 90%).
  • nitrogen oxides such as N0 or N0 2 as a nitrogen source are placed in the algae species at the initial culture density after one hour, and the culture is started. Samples were taken at regular intervals to determine biomass density. The breeding ended after 144 hours.
  • the fresh water species of Nannochloropsis 1 imnetica was selected and the initial culture density was 0. lg/L, the reactor liquid depth is 80cm (cross-sectional area is 2mX3m).
  • the culture medium is pre-added with trace amounts of ammonium phosphate, zinc, iron and other trace element fertilizers, and a certain amount of C0 2 (about 90%) and NO or N0 2 as nitrogen source are respectively introduced. Nitrogen oxides, cultured after 144 hours. Other culture conditions were the same as in Example 1 unless otherwise stated.
  • the fresh water species of Nannochloropsis 1 imnetica was selected and the initial culture density was
  • the reactor liquid depth is 80cm (the cross-sectional area is 2mX3m).
  • the culture medium is pre-added with trace amounts of ammonium phosphate, zinc, iron and other trace element fertilizers, and a certain amount of air and NO or N0 2 nitrogen oxides as nitrogen sources, 144 After the hour, the breeding is over.
  • Other culture conditions were the same as in Example 1 unless otherwise stated.
  • Example 1 and Example 2 the microalgae culture was carried out in different bioreactors in different stages and different stages were used in different stages.
  • Example 3 the microalgae were always cultured under the same conditions. The results of the three examples show that the total lipid content and total effective fat content of the microalgae cultured in stages are high, and the oil production efficiency is high;
  • Embodiment 1 uses red light as the light source
  • the second stage uses blue light
  • the second stage in Embodiment 2 uses blue light
  • the second stage uses red light.
  • the experimental results show that the breeding effect of Example 1 is better than that of Example 2. It can be considered that the use of light sources of different wavelengths in the first and second stages of microalgae cultivation for different algae species will result in a result of the region

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Abstract

L'invention concerne un bioréacteur comportant une source lumineuse incorporée, comprenant un récipient réacteur, un dispositif d'alimentation et d'évacuation de matières, un dispositif photoémetteur, un dispositif distributeur de nutriments et un distributeur de gaz, le récipient réacteur étant surmonté d'une plaque de couverture et servant à loger un liquide de culture pour la croissance de micro-algues ; le dispositif d'alimentation et d'évacuation de matière étant en relation d'étanchéité avec le récipient réacteur et portant une vanne et un interrupteur, et les micro-algues étant introduites dans le récipient réacteur ou extraites de ce dernier par pompage en passant par le dispositif d'alimentation et d'évacuation de matières ; le dispositif photoémetteur étant utilisé pour générer une source lumineuse requise pour la croissance des micro-algues ; le dispositif distributeur de nutriments étant utilisé pour introduire dans le récipient réacteur les nutriments requis pour la croissance des micro-algues ; et le distributeur de gaz étant utilisé pour introduire dans le récipient réacteur les gaz requis pour la croissance des micro-algues. Le bioréacteur comprenant la source lumineuse incorporée n'est pas affecté par les changements météorologiques, peut réaliser une reproduction des micro-algues par stades successifs, présente une bonne aptitude à la régulation et est avantageux pour la réalisation d'une production industrialisée stable et continue ; et, du fait du système de montage en série et/ou en parallèle du bioréacteur, la culture de micro-algues à grande échelle devient plus efficace.
PCT/CN2014/081312 2014-06-30 2014-06-30 Bioréacteur comportant une source lumineuse incorporée et procédé de culture de micro-algues WO2016000192A1 (fr)

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CN107083322A (zh) * 2017-06-23 2017-08-22 四川省天惠能源科技有限公司 一种多层微藻生长繁殖装置
CN107779395A (zh) * 2016-08-25 2018-03-09 国家开发投资公司 一种高通量微藻生长测试装置及方法
CN110713901A (zh) * 2019-11-08 2020-01-21 深圳市德和生物科技有限公司 一种微藻养殖光生物反应釜及含其的连续培养反应系统
CN110760439A (zh) * 2019-11-08 2020-02-07 深圳市德和生物科技有限公司 一种藻类养殖光生物反应釜及含其的连续培养反应系统

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US20100323436A1 (en) * 2007-11-28 2010-12-23 Choul-Gyun Lee Photobioreactor for large-scale culture of microalgal
CN201778022U (zh) * 2010-03-12 2011-03-30 江苏大学 一种富油微藻培养装置
CN104017726A (zh) * 2014-06-30 2014-09-03 张玟籍 一种内置光源生物反应器及微藻养殖方法

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US20100323436A1 (en) * 2007-11-28 2010-12-23 Choul-Gyun Lee Photobioreactor for large-scale culture of microalgal
CN201198474Y (zh) * 2008-04-02 2009-02-25 林健峯 油质性微藻培养装置
CN201778022U (zh) * 2010-03-12 2011-03-30 江苏大学 一种富油微藻培养装置
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107779395A (zh) * 2016-08-25 2018-03-09 国家开发投资公司 一种高通量微藻生长测试装置及方法
CN107083322A (zh) * 2017-06-23 2017-08-22 四川省天惠能源科技有限公司 一种多层微藻生长繁殖装置
CN110713901A (zh) * 2019-11-08 2020-01-21 深圳市德和生物科技有限公司 一种微藻养殖光生物反应釜及含其的连续培养反应系统
CN110760439A (zh) * 2019-11-08 2020-02-07 深圳市德和生物科技有限公司 一种藻类养殖光生物反应釜及含其的连续培养反应系统
CN110713901B (zh) * 2019-11-08 2020-11-24 安徽德宝生物科技有限公司 一种微藻养殖光生物反应釜及含其的连续培养反应系统
CN110760439B (zh) * 2019-11-08 2020-11-24 安徽德宝生物科技有限公司 一种藻类养殖光生物反应釜及含其的连续培养反应系统

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