WO2015192329A1 - 核酸提取仪的废液容器、核酸提取仪及控制方法 - Google Patents

核酸提取仪的废液容器、核酸提取仪及控制方法 Download PDF

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Publication number
WO2015192329A1
WO2015192329A1 PCT/CN2014/080128 CN2014080128W WO2015192329A1 WO 2015192329 A1 WO2015192329 A1 WO 2015192329A1 CN 2014080128 W CN2014080128 W CN 2014080128W WO 2015192329 A1 WO2015192329 A1 WO 2015192329A1
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Prior art keywords
liquid
waste liquid
nucleic acid
waste
chamber
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PCT/CN2014/080128
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English (en)
French (fr)
Inventor
王海
王光亮
蒋凯平
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Priority to CN201480074431.0A priority Critical patent/CN105940095A/zh
Priority to PCT/CN2014/080128 priority patent/WO2015192329A1/zh
Publication of WO2015192329A1 publication Critical patent/WO2015192329A1/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology

Definitions

  • the present invention relates to a nucleic acid extractor. Background technique
  • nucleic acid extraction technology puts forward very high requirements for cross-contamination, and it is necessary to use a shared device in the test. Therefore, the nucleic acid extractor needs to use a disposable consumable for nucleic acid extraction operation, and it is because of such a magnetic bead nucleic acid extractor.
  • the core is essentially a magnetic separation device and process that uses disposable consumables. In order to facilitate the automation of the instrument, the form of consumables used has become an important issue.
  • each test must provide a reaction vessel carrying the reaction liquid as a reaction vessel; secondly, during the magnetic separation process, multiple aspiration and liquid injection operations are required.
  • a disposable liquid suction head Tip
  • the container is arranged in the vicinity of the reaction cup for storing the waste liquid; in order to ensure the cleaning effect of the magnetic separation, it is usually required to perform the suction liquid operation multiple times, such as sucking the original reaction liquid of the reaction cup and sucking the cleaning liquid injected during the cleaning process.
  • the invention provides a novel nucleic acid extracting instrument, a waste liquid container and a control method.
  • the invention provides a waste liquid container of a nucleic acid extracting device, comprising a clamping surface, a waste liquid chamber for storing waste liquid, and a storage chamber for storing the liquid suction head, wherein one of the waste liquid chamber and the storage chamber has A support surface for supporting the liquid suction head.
  • the storage chamber may be located at one side or intermediate position of the waste liquid chamber, that is, on the circumference of the magnetic separation disc of the nucleic acid extractor, the storage chamber and the waste liquid chamber are staggered at an angle, thereby discharging waste liquid and storing to the waste liquid chamber.
  • the magnetic disk needs to be rotated by an angle.
  • the storage chamber may be located above the waste liquid chamber and communicated with each other to discharge waste into the waste liquid chamber When the liquid and the liquid suction head are unloaded to the storage chamber, the magnetic reaction disk does not need to be rotated by an angle.
  • a nucleic acid extractor comprising a magnetic separation disc, at least one liquid absorbing mechanism and at least one liquid filling mechanism, the magnetic separating disc having a plurality of positions for cooperating with the waste liquid container, the magnetic separating disc having at least one liquid absorbing position And at least one liquid injection position, the liquid suction mechanism is disposed at a corresponding liquid suction position, and the liquid injection mechanism is disposed at a corresponding liquid injection position.
  • a nucleic acid extractor comprising a magnetic separation disk driven by a first power device, a magnetic field ring driven by a second power device, at least one liquid absorption mechanism, and at least one liquid injection mechanism, the magnetic separation disks having the same circumference a plurality of first positions for mating with the cuvette and a plurality of second positions for mating with the waste container, the magnetic separation disk having at least one liquid suction position and at least one liquid injection position, the suction The liquid mechanism is disposed at a corresponding liquid suction position, and the liquid injection mechanism is disposed at a corresponding liquid injection position, and the magnetic field ring surrounds the magnetic separation disk.
  • the magnetic field ring and the magnetic separation disk can be kept in sync or relatively rotated.
  • the magnetic field ring may have a plurality of first portions capable of adsorbing magnetic beads and a plurality of second portions capable of adsorbing magnetic beads.
  • first portion is aligned with the reaction cup having the magnetic beads; when the adsorption is not required, the second portion is aligned with the reaction cup having the magnetic beads.
  • a method for controlling a nucleic acid extractor includes the following steps:
  • the liquid suction mechanism is lowered and the liquid suction head placed in the storage chamber of the waste liquid container is loaded;
  • the liquid suction mechanism is lowered, and the waste liquid in the liquid suction head is discharged into the waste liquid chamber of the waste liquid container and the liquid suction head is unloaded.
  • the beneficial effects of the invention are as follows:
  • the waste liquid container can simultaneously serve to store the waste liquid and store the liquid suction head, thereby effectively reducing the consumption of the liquid suction head, and effectively reducing the absorption of the waste liquid by the liquid suction mechanism. Moving distance. DRAWINGS
  • FIG. 1 is a schematic structural view of a first embodiment of a waste liquid container
  • FIG. 2 is a schematic structural view of a liquid suction head
  • Figure 3 is a schematic view showing the structure of the liquid suction head after being inserted into the waste liquid container of Figure 1;
  • FIG. 4 is a schematic structural view of a second embodiment of a waste liquid container
  • Figure 5 is a schematic structural view of a third embodiment of a waste liquid container
  • Figure 6 is a schematic view showing the structure of a nucleic acid extracting apparatus of the present embodiment
  • Fig. 7 is a view showing the positional distribution of a magnetic separation disk of the nucleic acid extractor of the present embodiment. detailed description
  • a waste liquid container 7 of a nucleic acid extractor can be used in combination with a reaction cup, which comprises a waste liquid chamber 1 and a storage chamber 2, which is used for storing during the test.
  • the waste chamber 2 is used for storing the liquid suction head 3.
  • the waste container also has a support surface 4 for supporting the liquid suction head 3.
  • the waste container also has a gripping surface 5 for gripping the gripping mechanism to transfer the waste container so that the waste container can be transferred between the different positions of the instrument as the reaction cup.
  • a clamping mechanism such as a robot.
  • the support surface 4 can be disposed in the waste chamber or in the storage chamber. Typically, the support surface is located in a storage chamber, such as the top end of the storage chamber. When storing the pipetting head, the pipetting head rests on the support surface and is inserted into the storage chamber.
  • the clamping surface 5 may be provided in the waste chamber or in the storage chamber. Usually, the clamping surface is provided in the moonlight of the waste liquid, the outer surface of the moon liquid of the mouth waste liquid, the outer peripheral surface 3 ⁇ 4 port cylinder surface, the cylindrical surface 3 ⁇ 4 port cylindrical surface or the square cylinder surface.
  • the top of the storage chamber 2 is open; the bottom of the storage chamber can be closed or open.
  • the top of the waste chamber 1 is open, and the bottom of the waste chamber is closed.
  • a waste liquid container of a nucleic acid extractor comprises a bottom closed bottom liquid waste chamber 1 and a bottom closed bottom storage chamber 2, and the storage chamber 2 is located in the middle of the waste liquid chamber 1. , the waste liquid chamber 1 is surrounded by the storage chamber 2.
  • the top end surface of the storage chamber 2 is a support surface 4 for supporting the liquid suction head, and the outer peripheral surface of the waste liquid chamber 1 is a clamping surface 5 which is convenient for the robot to grip.
  • the cross section of the storage chamber 2 may be circular, and the cross section of the waste liquid chamber 1 may be a circular shape, and the cross section refers to a section perpendicular to the axis of the storage chamber and the waste liquid chamber.
  • the waste liquid chamber and the storage chamber may be coaxial.
  • a waste liquid container of a nucleic acid extractor comprises a bottomed closed bottom liquid chamber 1 and a bottom closed bottomed storage chamber 2, and the storage chamber 2 is located at one side of the waste liquid chamber 1.
  • the top surface of the storage chamber is a support surface 4 for supporting the liquid suction head, and the outer circumferential surface of the storage chamber is a clamping surface 5.
  • the liquid suction head 3 rests on the support surface 4 and is inserted into the storage chamber 2; when gripping, the robot grips the upper portion of the outer peripheral surface of the storage chamber.
  • the top end surface of the storage chamber 2 is higher than the top end surface of the waste liquid chamber 1.
  • the axis of the waste chamber and the axis of the storage chamber may be parallel and eccentric.
  • the outer circumference of the storage chamber can be a circle Cylinder.
  • the outer peripheral surface of the waste chamber may be a non-cylindrical surface, such as a polygonal cylinder.
  • a waste liquid container of a nucleic acid extractor comprises a storage chamber 2 with an open bottom and a waste liquid chamber 1 closed at the bottom.
  • the storage chamber 2 is located above the waste liquid chamber 1, and the bottom of the storage chamber is waste.
  • the top of the liquid chamber is connected.
  • the gripping surface 5 is the outer peripheral surface of the top of the waste container.
  • the support surface 4 can be the top end of the storage chamber. The waste liquid can flow into the waste liquid chamber through the storage chamber.
  • the waste liquid chamber and the storage chamber may be coaxial.
  • the axis of the waste chamber and the axis of the storage chamber can also be arranged parallel and eccentrically.
  • the waste liquid container is mainly used in a nucleic acid extractor, and the nucleic acid extractor includes a magnetic separation disk 12 as a core component for carrying a reaction cup 6 requiring magnetic separation and
  • the used waste liquid container 7 is used for carrying the liquid suction head and storing the waste liquid; and further comprises a liquid suction mechanism 8 and a liquid injection mechanism 9 arranged above the operation track of the reaction cup, wherein the liquid absorption mechanism has a total Arrange four, a total of two liquid injection mechanisms, the liquid suction mechanism needs to load the liquid suction head to absorb the liquid in the reaction cup; and a magnetic field ring disposed outside the magnetic separation disk that can rotate concentrically with the magnetic separation disk 10, a magnet 11 is mounted at a fixed position of the magnetic field ring, and the magnet is used to adsorb the magnetic beads in the reaction cup.
  • the magnetic separation discs are arranged in thirty-six positions for respectively carrying the reaction cup and the waste liquid container, wherein the reaction cup and the waste liquid container are separated into the magnetic separation.
  • the thirty-six positions are defined as the first position, the second position, the third position, and the 36th position.
  • the reaction cup and the waste container are placed in the magnetic separation disc from the position A of the magnetic separation disc No. 36, and then the magnetic separation disc is rotated, and the reaction cup corresponds in the process.
  • the outer magnetic field ring has a magnetic field, so that the magnetic beads are adsorbed to the side of the reaction cup; when the waste liquid container rotates with the magnetic separation disk to position No.
  • the magnetic separation disc After sucking up the liquid in the reaction cup, it is lifted up; then the magnetic separation disc continues to advance to a position such that the waste liquid container is located at position 9 on the magnetic separation disc, and then the liquid suction mechanism moves downward to discharge the waste liquid and unload the liquid suction head (at In this process, since the position of the waste liquid discharged from the waste liquid container is inconsistent with the position of the unloading liquid suction head, it is necessary to move the magnetic separation disk to the position of unloading the liquid suction head after the waste liquid is discharged, and of course, the waste liquid capacity can also be passed.
  • the design of the device is such that the two positions are kept the same, the step of moving the magnetic separation disk is not required, the design is as shown in FIG.
  • the magnetic separation disk and The magnetic field ring of its outer ring keeps moving or stopping synchronously, so the cuvette is always in the magnetic field In the state of adsorption.
  • the magnetic separation disc continues to advance to a position such that when the reaction cup enters the position No. 11 (ie, below the liquid injection mechanism) on the magnetic separation disc, during the advancement of the magnetic separation disc, the magnetic field loop The relative movement with the magnetic field on the magnetic field ring corresponds to the reaction cup.
  • the liquid injection mechanism injects the cleaning liquid into the reaction cup without magnetic field adsorption, and then the magnetic separation disk retreats to a position to make the waste liquid container re-return.
  • the aspirating mechanism descends to load the liquid-collecting head, and then the magnetic separation disk continues to retreat to a position such that the reaction position is below the first-stage aspiration position, and the aspirating mechanism drops the cleaning liquid in the reaction cup.
  • the magnetic beads are lifted and removed, and then the magnetic separation disc continues to advance to a position, so that the waste liquid container is located under the liquid suction mechanism to unload the liquid suction head, and the magnetic separation first-stage liquid injection and mixing operation is completed.
  • the magnetic separation disk and the magnetic field ring keep moving synchronously or stop, so the reaction cup is always in the state of no magnetic field adsorption, and the magnetic beads are resuspended in the process. Lotion.
  • the magnetic field ring is re-rotated relative to the magnetic separation disc so that the reaction cup is in a magnetic field, and the next-order magnetic field adsorption is continued.
  • the second-order magnetic separation and liquid-filling action are repeated with the above process, and then the third stage only performs the liquid absorption action, and does not perform the liquid injection action, sucking the liquid in the reaction cup, the fourth order is the third
  • the steps are repeated, and only the liquid is absorbed by the liquid to absorb the residual liquid at the bottom of the cup.
  • the cuvette and waste container continue to move back to position 36 as the magnetic separation disk moves, and the reaction cup and waste container are removed by the instrument for subsequent operations.
  • the symbol D refers to the 17th position, that is, the second-stage liquid absorption position
  • the label E refers to the 19th position, that is, the second-stage liquid injection position
  • the reference F refers to the 25th position, that is, the third-order liquid absorption Position
  • Reference G refers to position 33, the fourth-order aspiration position.
  • the above process is designed for a specific extraction characteristic. Depending on the degree of cleanliness required for extraction and the difference in residual liquid, the number of times of aspiration and infusion can be increased or decreased, even at the end of the process. Other reaction liquid components are not described in detail here.
  • the number of liquid suction heads required for each test is reduced to one, and it is not necessary to apply one liquid suction head per liquid suction. It also greatly reduces the large displacement of the liquid suction mechanism after taking up the waste liquid.
  • a nucleic acid extractor includes a magnetic separation disk 12, a magnetic field ring 10, at least one liquid absorbing mechanism 8, and at least one liquid injection mechanism 9, and a plurality of magnetic distribution disks are distributed on the same circumference.
  • a first position for placing a cuvette 6 having magnetic beads and a second position for placing the waste liquid container 7 having at least one liquid-absorbent position and at least one liquid-filling position,
  • the liquid suction mechanism is disposed at a corresponding liquid suction position
  • the liquid injection mechanism is disposed at a corresponding liquid injection position
  • the magnetic separation disk 12 is driven by the first power mechanism to be rotatable.
  • the magnetic field ring 10 is driven by the second power mechanism to be rotatable.
  • the magnetic field ring 10 can be synchronized or stationary with the magnetic separation disk 12 or can be rotated relative to each other.
  • the magnetic field ring can surround the magnetic separation disk.
  • the magnetic field ring has a first portion with magnetic properties and a second portion with no magnetic properties. The first portion can magnetically adsorb the magnetic beads in the corresponding reaction cup, and the second portion cannot The magnetic beads in the corresponding reaction cups are magnetically adsorbed.
  • the nucleic acid extractor may further include a platform 13 fixed to the magnetic separation disk 12, and the liquid injection mechanism 9 and the liquid suction mechanism 8 are both fixed to the platform 13, and the platform is provided with a through hole 14 through which the liquid suction head passes.
  • a nucleic acid extractor in another embodiment, includes a magnetic separation disc, at least one liquid absorbing mechanism, and at least one liquid injection mechanism, the magnetic separation disc having a plurality of positions for cooperating with the waste liquid container, magnetic The separation disc has at least one liquid absorption position and at least one liquid injection position, the liquid suction mechanism is disposed at a corresponding liquid absorption position, and the liquid injection mechanism is disposed at a corresponding liquid injection position.
  • the magnetic separation disc can be fixed or can be driven by the power unit to be rotatable.
  • the positions of the respective waste container can be distributed on the magnetic separation disk in the same circumference, and the positions can also be distributed in the same ellipse, or distributed in the same polygon, or distributed in an irregular closed shape, that is, each position is formed on the magnetic separation disk. ring.
  • the magnetic field ring may surround the magnetic separation disk.
  • the magnetic separation disc may have a first position that mates with the reaction cup and a second position that mates with the waste container, and the first position and the second position may be spaced apart.
  • the magnetic field ring can be fixed or can be driven by another power unit to rotate. The magnetic field ring can rotate concentrically with the magnetic separation disk.
  • the first and second positions may be distributed on the magnetic separation disk in the same circumference, and each position may also be distributed in the same ellipse, or distributed in the same polygon, or distributed in an irregular closed shape, that is, the first and second positions are on the magnetic separation disk. In a circle.
  • a method for controlling a nucleic acid extractor includes the following steps:
  • the pipetting mechanism is lowered and the pipetting head placed in the waste container is loaded.
  • the liquid suction mechanism is lowered, and the liquid suction head in the waste liquid container is loaded under the liquid suction mechanism, and then lifted up. After the completion, the aspirating mechanism is lifted with the liquid suction head.
  • the liquid suction mechanism is lowered, the waste liquid in the liquid suction head is discharged into the waste liquid chamber of the waste liquid container, and the liquid suction head is unloaded. After the unloading is completed, the liquid suction head is placed in the storage chamber of the waste container.

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Abstract

一种核酸提取仪的废液容器（7）、核酸提取仪及控制方法，包括用于存放废液的废液腔（1）和用于存放吸液头（3）的存放腔（2），所述废液腔和存放腔中之一具有用于支撑所述吸液头的支撑面（4），且所述废液腔和存放腔中之一具有夹持面（5）。废液容器可以同时起到存放废液和存放吸液头的作用，从而能够有效减少吸液头的消耗量，也有效减小了吸液机构吸走废液后的移动距离。

Description

核酸提取仪的废液容器、 核酸提取仪及控制方法 技术领域
本发明是关于一种核酸提取仪。 背景技术
由于核酸检测技术灵敏度高， 理论上讲只需要一条目标核酸片段即 可检出阳性结果。 由此核酸提取技术对交叉污染提出了非常高的要求， 需要测试间不能釆用共有器件， 因此核酸提取仪需要釆用一次性耗材进 行核酸提取操作， 也正是因为如此磁珠法核酸提取仪的核心本质上是釆 用一次性耗材的磁分离装置及过程。 为了便于仪器自动化运行， 所釆用 的耗材形式成为了一个重要的问题。
首先， 磁分离过程中， 每个测试必须提供一个反应杯承载反应液作 为反应的进行容器； 其次， 磁分离过程中， 需要多次的吸液及注液的操 作。 对于吸液而言， 由于需要和反应杯内的液体接触， 因此必须釆用一 次性的吸液头（Tip); 为了避免吸液头吸取废液后长距离的转移废液， 还 需要提供一个容器布置在反应杯的附近用于存放废液； 为了保证磁分离 的清洗效果， 通常需要多次进行吸注液操作， 如吸取反应杯原有的反应 液、 吸取清洗过程中注入的清洗液， 而且清洗液也很可能是多次注入。 因此如果每次吸液都釆用一个全新的吸液头则需要较多的吸液头消耗 量， 一方面增加了使用成本， 另一方面仪器上也需要开辟更大的空间来 存放耗材。 发明内容
本发明提供一种新的核酸提取仪、 废液容器及控制方法。
本发明提供一种核酸提取仪的废液容器， 包括夹持面、 用于存放废 液的废液腔和用于存放吸液头的存放腔， 所述废液腔和存放腔中之一具 有用于支撑所述吸液头的支撑面。
存放腔可以位于废液腔的一侧或中间位置， 即相当于在核酸提取仪 磁分离盘的圓周上， 存放腔和废液腔错开一个角度， 从而在向废液腔排 放废液和向存放腔卸载吸液头时， 磁反应盘需要转动一个角度。
存放腔可以位于废液腔的上方且两者连通， 从而在向废液腔排放废 液和向存放腔卸载吸液头时， 磁反应盘不需要转动一个角度。
一种核酸提取仪， 包括一个磁分离盘、 至少一个吸液机构及至少一 个注液机构， 磁分离盘具有多个用于与上述废液容器配合的位置， 磁分 离盘具有至少一个吸液位置和至少一个注液位置， 吸液机构设于与其对 应的吸液位置， 注液机构设于与其对应的注液位置。
一种核酸提取仪， 包括由第一动力装置驱动的磁分离盘、 由第二动 力装置驱动的磁场环、 至少一个吸液机构及至少一个注液机构， 所述磁 分离盘具有同圓周分布的多个用于与反应杯配合的第一位置和多个用 于与所述废液容器配合的第二位置， 所述磁分离盘具有至少一个吸液位 置和至少一个注液位置， 所述吸液机构设于与其对应的吸液位置， 所述 注液机构设于与其对应的注液位置， 所述磁场环环绕所述磁分离盘。
磁场环和磁分离盘可以保持同步， 也可以相对转动。 磁场环可以具 有能够吸附磁珠的多个第一部位和不能吸附磁珠的多个第二部位。 需要 吸附磁珠时， 使第一部位对准有磁珠的反应杯； 不需要吸附时， 使第二 部位对准有磁珠的反应杯。
一种核酸提取仪的控制方法， 包括如下步骤：
装载步骤， 吸液机构下降并装载搁放在废液容器的存放腔中的吸液 头； 体；
卸载步骤， 所述吸液机构下降， 将所述吸液头中的废液排放到所述 废液容器的废液腔内并卸载所述吸液头。
本发明的有益效果是： 废液容器可以同时起到存放废液和存放吸液 头的作用， 从而能够有效减少吸液头的消耗量， 也有效减小了吸液机构 吸走废液后的移动距离。 附图说明
图 1是废液容器第一具体实施方式的结构示意图；
图 2是吸液头的结构示意图；
图 3是吸液头插入图 1中废液容器后的结构示意图;
图 4是废液容器第二具体实施方式的结构示意图；
图 5是废液容器第三具体实施方式的结构示意图； 图 6是本实施方式核酸提取仪的结构示意图；
图 7是本实施方式核酸提取仪的磁分离盘的位置分布示意图。 具体实施方式
如图 1至图 5所示， 一种核酸提取仪的废液容器 7 , 能够与反应杯 配合使用， 其包括废液腔 1和存放腔 2 , 该废液腔 1用于存放测试过程 中产生的废液， 该存放腔 2用于存放吸液头 3。 该废液容器还具有支撑 面 4 , 该支撑面 4用于支撑吸液头 3。 该废液容器还具有夹持面 5 , 该夹 持面用于供夹持机构夹持来转移废液容器， 使得废液容器可以随着反应 杯在仪器的不同位置间转移。 夹持机构如机械手。
支撑面 4可以设于废液腔， 也可以设于存放腔。 通常的， 支撑面设 于存放腔， 如存放腔的顶端面。 存放吸液头时， 吸液头搁放在该支撑面 上并插入存放腔内。
夹持面 5可以设于废液腔， 也可以设于存放腔。 通常的， 夹持面设 于废液月空， 口废液月空的夕卜周面， 该外周面 ¾口柱面， 该柱面 ¾口圓柱面或方 柱面等。
存放腔 2的顶部敞口； 存放腔的底部可以封闭， 也可以敞口。 废液 腔 1的顶部敞口， 废液腔的底部封闭。
如图 1至图 3所示， 一种核酸提取仪的废液容器， 包括底部封闭的 有底废液腔 1和底部封闭的有底存放腔 2 , 存放腔 2位于废液腔 1的中 间位置， 使废液腔 1环绕存放腔 2。 存放腔 2的顶端面是用于支撑吸液 头的支撑面 4 ,废液腔 1的外周面是夹持面 5 ,该夹持面便于机械手夹持。 存放腔 2的横截面可以是圓形， 废液腔 1的横截面可以是圓环形， 横截 面是指与存放腔、 废液腔的轴线垂直的截面。
本实施方式中， 废液腔和存放腔可以同轴线。
如图 4所示， 一种核酸提取仪的废液容器， 包括底部封闭的有底废 液腔 1和底部封闭的有底存放腔 2 , 存放腔 2位于废液腔 1的一侧。 存 放腔的顶端面是支撑吸液头的支撑面 4 ,存放腔的外周面是夹持面 5。存 放时， 吸液头 3搁放于支撑面 4并插入存放腔 2内； 夹持时， 机械手夹 持存放腔外周面的上部。
本实施方式中， 存放腔 2的顶端面高于废液腔 1的顶端面。 废液腔 的轴线和存放腔的轴线可以平行并偏心设置。 存放腔的外周面可以是圓 柱面。 废液腔的外周面可以是非圓柱面， 如多边形柱面。
如图 5所示， 一种核酸提取仪的废液容器， 包括底部敞口的存放腔 2和底部封闭的废液腔 1 ,存放腔 2位于废液腔 1的上方，存放腔的底部 与废液腔的顶部连通。 夹持面 5是废液容器顶部的外周面。 支撑面 4可 以是存放腔的顶端面。 废液能够经存放腔流入废液腔。
本实施方式中， 废液腔和存放腔可以同轴线。 当然， 废液腔的轴线 和存放腔的轴线也可以平行并偏心设置。
如图 6及图 7所示， 废液容器主要应用于核酸提取仪中， 核酸提取仪 包括作为核心组件的磁分离盘 12 , 该磁分离盘用于承载需要磁分离的反 应杯 6以及与之配套使用的废液容器 7 , 废液容器的作用是用来承载吸液 头以及存放废液；还包括布置在反应杯运行轨迹上方的吸液机构 8以及注 液机构 9 , 其中吸液机构共布置四个， 注液机构共布置两个， 吸液机构需 要装载吸液头后才可以吸取反应杯中的液体； 还包括布置在磁分离盘外 侧的一个可与磁分离盘同心旋转的磁场环 10 , 磁场环的固定位置安装有 磁铁 11 , 该磁铁用于吸附反应杯中的磁珠。
在一个具体实施方式中，如图 7所示，磁分离盘上均勾排布有三十六 个位置， 分别用于承载反应杯和废液容器， 其中反应杯和废液容器间隔 进入磁分离盘， 该三十六个位置分别被定义为第 1号位置、 第 2号位置、 第 3号位置…第 36号位置。在工作开始时，反应杯和废液容器被仪器从磁 分离盘上第 36号位置 A—前一后放入磁分离盘， 然后随着磁分离盘旋转 递进， 在此过程中反应杯对应的外侧磁场环位置具有磁场， 使得磁珠被 吸附到反应杯一侧；当废液容器随着磁分离盘旋转到磁分离盘上第 9号位 置 B (即第一阶的吸液机构下方）时，吸液机构向下运动装载废液容器中的 吸液头， 然后抬起； 其后磁分离盘向后退一个位置， 使得反应杯刚好进 入磁分离盘上第 9号位置， 吸液机构下降吸取反应杯中的液体后抬起； 然 后磁分离盘继续前进一个位置， 使得废液容器位于磁分离盘上第 9号位 置， 然后吸液机构向下运动排出废液并卸载吸液头 （在此过程中， 由于 废液容器内排出废液位置与卸载吸液头位置并不一致， 因此需要排完废 液后磁分离盘再次运动到卸载吸液头的位置， 当然也可以通过废液容器 的设计， 使得两个位置保持一致， 则不需要磁分离盘运动的步骤， 该设 计如图 5所示）， 至此磁分离第一阶吸液动作完成， 在上述过程中， 磁分 离盘和其外圈的磁场环保持同步运动或停止， 因此反应杯一直处于磁场 吸附状态下。 完成第一阶吸液动作后， 磁分离盘继续前进一个位置， 使 得反应杯进入到磁分离盘上第 11号位置 C (即注液机构下方）时，在磁分离 盘前进过程中， 磁场环与之发生相对运动使得磁场环上没有磁场的部分 对应反应杯， 此时注液机构在没有磁场吸附作用下向反应杯内注入清洗 液，其后磁分离盘回退一个位置使得废液容器重新回到第一阶吸液位置， 吸液机构下降装载吸液头， 然后磁分离盘继续后退一个位置， 使得反应 位置于第一阶吸液位置下方， 吸液机构下降对反应杯内的清洗液和磁珠 进行吸排混勾后抬起， 然后磁分离盘继续前进一个位置， 使得废液容器 位于吸液机构下方卸载吸液头，至此磁分离第一阶注液及混匀动作完成， 在此过程中磁分离盘与磁场环一直保持同步运动或停止， 因此反应杯一 直处于无磁场吸附的状态下， 便于磁珠重新悬浮于清洗液中。 完成混匀 后磁场环重新与磁分离盘相对转动使得反应杯处于有磁场的状态下， 继 续下一阶的磁场吸附。 第二阶的磁分离吸液及注液混勾动作与上述过程 重复， 然后第三阶则只进行吸液动作， 不进行注液动作， 吸干反应杯内 的液体， 第四阶同第三阶动作重复， 不注液只吸液进一步吸干杯底的残 留液体。 至此， 整个磁分离动作完成。 然后， 反应杯和废液容器随着磁 分离盘继续运动回到第 36号位置， 由仪器取走反应杯和废液容器进行后 续的操作。 图 7中， 标号 D指第 17号位置， 即第二阶吸液位置； 标号 E指 第 19号位置， 即第二阶注液位置； 标号 F指第 25号位置， 即第三阶吸液 位置； 标号 G指第 33号位置， 即第四阶吸液位置。
上述过程是针对一个特定的提取特性而设计， 根据提取所需要的洁 净程度的不同以及对残留液体的不同， 可以增加或者减少吸液和注液的 次数， 甚至在最后增加注入后续测试所需要的其他反应液成分， 此处不 做详细描述。 但从上述实施方式来看， 由于釆用了该结构的废液容器使 得每个测试所需要的吸液头数量减少到一个， 而不需要每次吸液应用一 个吸液头。 也大大减小了吸液机构吸取废液后的移动 巨离。
如图 6及图 7 所示， 一种核酸提取仪， 包括磁分离盘 12、 磁场环 10、 至少一个吸液机构 8及至少一个注液机构 9 , 磁分离盘上同圓周分 布有多个第一位置和第二位置， 第一位置用于放置具有磁珠的反应杯 6 , 第二位置用于放置上述废液容器 7 ,磁分离盘 12具有至少一个吸液位置 和至少一个注液位置， 吸液机构设于与其对应的吸液位置， 注液机构设 于与其对应的注液位置， 磁分离盘 12由第一动力机构驱动而能够转动， 磁场环 10由第二动力机构驱动而能够转动。 磁场环 10可以和磁分离盘 12保持同步或静止， 也可以相对转动。 磁场环可以环绕磁分离盘， 磁场 环具有带有磁性的第一部位和未带有磁性的第二部位， 第一部位能够对 其对应的反应杯内的磁珠产生磁力吸附， 第二部分不能对其对应的反应 杯内的磁珠产生磁力吸附。
核酸提取仪还可以包括一个固定于磁分离盘 12上方的平台 13 , 注 液机构 9和吸液机构 8均固定于平台 13上，且平台上设有供吸液头通过 的通孔 14。
在另一种实施方式中， 一种核酸提取仪， 包括一个磁分离盘、 至少 一个吸液机构及至少一个注液机构， 磁分离盘具有多个用于与上述废液 容器配合的位置，磁分离盘具有至少一个吸液位置和至少一个注液位置， 吸液机构设于与其对应的吸液位置 ,注液机构设于与其对应的注液位置。 磁分离盘可以固定， 也可以由动力装置驱动而能够旋转。 各与废液容器 配合的位置可以同圓周分布于磁分离盘， 各位置也可以同椭圓分布， 或 者同多边形分布， 或者同不规则封闭形状分布， 即各位置在磁分离盘上 围成一圈。
对于不同类型的核酸提取仪， 可以仅具有磁分离盘， 也可以同时具 有磁分离盘和磁场环， 磁场环可以环绕磁分离盘。 磁分离盘可以具有与 反应杯配合的第一位置和与废液容器配合的第二位置， 第一位置和第二 位置可以相间分布。 磁场环可以固定， 也可以由另一动力装置驱动而能 够旋转。 磁场环可以和磁分离盘同心旋转。 各第一、 第二位置可以同圓 周分布于磁分离盘， 各位置也可以同椭圓分布， 或者同多边形分布， 或 者同不规则封闭形状分布， 即第一、 第二位置在磁分离盘上围成一圈。
一种核酸提取仪的控制方法， 包括如下步骤：
装载步骤， 吸液机构下降并装载搁放在废液容器中的吸液头。 本步 骤中， 废液容器随着磁分离盘运动到预设位置后， 吸液机构下降， 将废 液容器中的吸液头装载到吸液机构的下方， 然后上抬。 完毕后， 吸液机构带着吸液头上抬。
卸载步骤， 吸液机构下降， 将吸液头中的废液排放到废液容器的废 液腔内并卸载所述吸液头。 卸载完毕后， 吸液头搁放于废液容器的存放 腔。 以上内容是结合具体的实施方式对本发明所作的进一步详细说明， 不能认定本发明的具体实施只局限于这些说明。 对于本发明所属技术领 域的普通技术人员来说， 在不脱离本发明构思的前提下， 还可以做出若 干简单推演或替换。

Claims

权 利 要 求
1. 一种核酸提取仪的废液容器， 其特征在于， 包括夹持面、 用于存 放废液的废液腔和用于存放吸液头的存放腔， 所述废液腔和存放腔中之 一具有用于支撑所述吸液头的支撑面。
2. 如权利要求 1所述的核酸提取仪的废液容器， 其特征在于， 所述 支撑面设于所述存放腔。
3. 如权利要求 2所述的核酸提取仪的废液容器， 其特征在于， 所述 支撑面是所述存放腔的顶端面。
4. 如权利要求 1所述的核酸提取仪的废液容器， 其特征在于， 所述 夹持面是所述废液腔的外周面或存放腔的外周面。
5. 如权利要求 4所述的核酸提取仪的废液容器， 其特征在于， 所述 外周面是圓柱面或多边形柱面。
6. 如权利要求 1所述的核酸提取仪的废液容器， 其特征在于， 所述 存放腔位于所述废液腔的中间位置并被所述废液腔环绕。
7. 如权利要求 1所述的核酸提取仪的废液容器， 其特征在于， 所述 存放腔位于所述废液腔的一侧。
8. 如权利要求 1所述的核酸提取仪的废液容器， 其特征在于， 所述 存放腔位于所述废液腔的上方， 所述存放腔的底部与所述废液腔的顶部 连通， 且所述废液腔的底部封闭。
9. 如权利要求 8所述的核酸提取仪的废液容器， 其特征在于， 所述 存放腔和所述废液腔同轴线。
10.—种核酸提取仪， 其特征在于， 包括一个磁分离盘、 至少一个吸 液机构及至少一个注液机构 ,所述磁分离盘具有多个用于与权利要求 1 -9 中任意一项所述的废液容器配合的位置， 所述磁分离盘具有至少一个吸 液位置和至少一个注液位置 , 所述吸液机构设于与其对应的吸液位置 , 所述注液机构设于与其对应的注液位置。
11.如权利要求 10所述的核酸提取仪， 其特征在于，还包括磁场环， 所述磁场环环绕所述磁分离盘。
12.如权利要求 10所述的核酸提取仪， 其特征在于， 所述磁分离盘 还具有多个与反应杯配合的位置。
13.如权利要求 12所述的核酸提取仪， 其特征在于， 所述与反应杯 配合的位置和所述与废液容器配合的位置相间分布。
14.如权利要求 10所述的核酸提取仪， 其特征在于， 所述磁分离盘 的上方固定有平台， 所述吸液机构和注液机构均固定于所述平台， 所述 平台具有上下贯穿的通孔。
15.—种核酸提取仪的控制方法， 其特征在于， 包括如下步骤： 装载步骤， 吸液机构下降并装载搁放在废液容器的存放腔中的吸液 头； 体；
卸载步骤， 所述吸液机构下降， 将所述吸液头中的废液排放到所述 废液容器的废液腔内并卸载所述吸液头。
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