WO2015150934A1 - Système de peptides à perméabilité cellulaire permettant de traiter des maladies provoquées par l'excitotoxicité du glutamate - Google Patents

Système de peptides à perméabilité cellulaire permettant de traiter des maladies provoquées par l'excitotoxicité du glutamate Download PDF

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WO2015150934A1
WO2015150934A1 PCT/IB2015/051640 IB2015051640W WO2015150934A1 WO 2015150934 A1 WO2015150934 A1 WO 2015150934A1 IB 2015051640 W IB2015051640 W IB 2015051640W WO 2015150934 A1 WO2015150934 A1 WO 2015150934A1
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peptide
glutamate
sequence
solution
peptide system
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PCT/IB2015/051640
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Marco FELIGIONI
Robert Giovanni NISTICO'
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Feligioni Marco
Nistico' Robert Giovanni
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Priority to EP15715423.8A priority Critical patent/EP3125917B1/fr
Priority to KR1020167030919A priority patent/KR20160138297A/ko
Priority to US15/301,760 priority patent/US9901618B2/en
Priority to CN201580029928.5A priority patent/CN106414504B/zh
Priority to JP2017503197A priority patent/JP2017516493A/ja
Publication of WO2015150934A1 publication Critical patent/WO2015150934A1/fr
Priority to US15/657,238 priority patent/US10398756B2/en

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Definitions

  • Cell-permeable peptide system for treating diseases caused by glutamate excitotoxicity is a cell-permeable peptide system for treating diseases caused by glutamate excitotoxicity
  • the present invention refers to the medical field. More in detail, the present invention refers to a new peptide system capable of blocking the NMDA-induced presynaptic release of glutamate.
  • the present peptide system is adapted for being used for treating pathologies associated with glutamate excitotoxicity.
  • GABA gamma-aminobutyric acid
  • L-glutamate is considered to be the principal mediator of the excitatory signal and of the quick synaptic transmission in the central nervous system.
  • L-glutamate plays a fundamental role in the development of the central nervous system (CNS), in the formation and elimination of the synapses, and in ceil migration, differentiation and death.
  • CNS central nervous system
  • L- glutamate is an important molecule for the transmission of the signal also in peripheral organs and tissues, as well as in endocrine ceils.
  • L-glutamate When it is freed at the physiological level, L-glutamate has a fundamental role in cerebral function and seems to be involved in many aspects of normal brain function, including cognition, memory and learning. Paradoxically, when L-glutamate reaches high levels in the synaptic space, it becomes highly toxic. It is therefore necessary to have an equilibrium of free L-glutamate as neurotransmitter and L-glutamate that has been removed and degraded from the synaptic space. If this equilibrium is well -controlled, it maintains the normal physiology of the synaptic transmission. When this equilibrium is, following some cause, irabalanced towards the persistence of L-glutamate in the synaptic space, this event leads to a toxic state that is very dangerous for cell survival. This pathological state is defined "glutamate excito toxicity" and it exclusively regards the Central Nervous System (CNS) notwithstanding the ubiquitous nature of this amino acid.
  • CNS Central Nervous System
  • the glutamatergic synaptic transmission occurs due to the presence of the L-glutamate receptors (GiuRs) on the CNS cells. These receptors are divided into two main categories: ionotropic (iGluRs) and metabotropic (mGiuRs) glutamatergic receptors.
  • the ionotropic receptors are a set of proteins (subunits) which form an ionic channel on the cell membrane and transduce the signal by opening and flowing the ions outward and inward.
  • the metabotropic receptors are proteins that traverse the cell membrane, do not allow molecules to pass through the membrane but transfer the outside signal, starting a series of intracellular events (second messengers).
  • NMDA, AMPA and kainate (KA) constitute part of the group of ionotropic receptors.
  • the group of metabotropic receptors is marked as mGiuRs and 8 different proteins thereof are known.
  • iGluRs ionotropic receptors of glutamate
  • This form of neurotoxicity is characterized by an excessive activation of iGluRs pre-synaptic and post-synaptic, which involve the entrance within the neuron of great quantities of Na + which is passively followed by the CP ion and an outflow of K + ions.
  • This movement of ions is responsible for the entrance of water, which causes neuronal "swelling", osmotic lysis and necrosis.
  • Ca ⁇ completes the ionic movement.
  • glutamatergic receptors responsible for the presynaptic release of glutamate there is that known as NMDA. This receptor was the first glutamatergic receptor discovered and characterized.
  • NMDA takes its name from the chemical substance that pharmacologically activates it in a specific manner, that is: N-Methyl D- Aspartate. It is a channel receptor activated by a ligand formed by 4 subunits which together create a pore in the cell membrane. Its localization in the neuron is both postsynaptic and presynaptic.
  • the NMDA channel has the particular characteristic of being normally blocked by the Magnesium ion. This "Magnesium block” does not occur when there is a depolarization of the neuron, for example when the other receptors of glutamate (AMPA, metabotropic) are activated.
  • AMPA glutamate
  • the NMDA receptors are constituted by an assembly of multiple subunits: GLUN1 and GLUN2. The latter is in turn divided into other isoforms (2A-B-C-D).
  • the composition in subunits is variable in the various parts of the brain and in the subcellular localization, but GLUN1 presence is required for normal receptor functionality.
  • the NMDA receptor complex has numerous different bond sites for different molecules that can modulate - since they are co- agonists together with glutamate - the opening of the channel and hence the intensities of the cellular response to this event.
  • modulator sites are that of glutamate (also site of NMDA), of glycine and of polyamines (spermine and spermidine).
  • Glutamate in addition to being essential for the life of neurons, can also become quite toxic.
  • the neurotoxicity of glutamate can also be involved in the pathogenesis of the various chronic diseases that are classified in the context of neurodegeneration and neuroinflammation.
  • the neurotoxicity of glutamate can occur through an aberrant stimulation of the NMDA, AMPA, KA receptors or the group I of the metabotropic receptors of glutamate.
  • the relative contribution of these different classes of receptors varies in accordance with the neurons involved and a variety of other circumstances.
  • Glutamate by means of the excessive activation of such receptors, appears to carry out an important role in cellular death following various attacks, such as those associated with epileptic states, cerebral ischemia, perinatal asphyxia and cranial trauma. Still more specifically, it seems that the NMDA receptor is that which is more greatly involved in these pathologies; indeed, experimentally, by using NMDA antagonists it has been possible to reduce the size of the cerebral damage associated with these pathologies.
  • NMDA amyotrophic lateral sclerosis
  • AMPA amyotrophic lateral sclerosis
  • NMDA multiple sclerosis
  • AD Alzheimer's disease
  • Parkinson's disease Drugs for clinical use are on the market today, both for acute and chronic pathologies. Their activity is often that of NMDA receptor antagonist.
  • This receptor block if on one hand appears effective and gives an initial remedi in some of these pathologies, on the other hand produces side effects that may even be severe. Medical research must therefore do its utmost to continue to identify the intracellular partners of the receptors, in this specific case NMDA, in a manner so as to render the action of possible drugs increasingly specific and less toxic.
  • AD Alzheimer's disease
  • the onset is progressive and develops from a young age, when the cognitive functions undergo a slight decline.
  • beta-amyloid ( ⁇ ) peptides produced in excess by the amyloidogenic processing of the amyloid precursor protein ( ⁇ ), are the first factor of the cause of AD.
  • the neurofibrillary tangles are another histopathological characteristic of the pathology.
  • the protein most present in these tangles is the protein associated with the microtubules (Tau) in its hyperphosphorylated form. These intercellular aggregates are present in various neurodegenerative pathologies, which are called tauopathies. The hyperphosphorylation of this protein was indicated as the cause of the neuronal degeneration in these pathologies.
  • Parkinson's disease (PD) is a neurodegenerative pathology in which specific areas of the central nervous system are damaged by mainly hitting the motor system with severe deambulation problems. This type of deficiency is attributed to the progressive degeneration of the dopaminergic neurons of the black substance that project to the striated body.
  • PD is characterized by the accumulation of Lewy bodies, which are protein aggregates in which alpha- synuclein and ubiquitin can also be recognized among other elements.
  • Lewy bodies proteins aggregates in which alpha- synuclein and ubiquitin can also be recognized among other elements.
  • the functional loss of DJ- 1 is one of the causes of the earlv onset of PD.
  • ALS Amyotrophic lateral sclerosis
  • the pathology starts with great exhaustion, which leads to muscular atrophy and progressively muscular paralysis is developed.
  • SLA is normally of sporadic origin but a percentage of hereditary forms is recognized.
  • SODl superoxide dismutase
  • Huntington's disease is a neurodegenerative pathology with hereditary character, which falls within the category of pathologies from polyglutamine. The gradual atrophy of the striatal neurons is the main characteristic in this pathology, which leads to having very well known deambulation deficiencies.
  • HD is the result of the elongation in polyQ of the N -terminal chain of the Huntingtin protein (HTT), which is not degraded as such and is accumulated in the neurons.
  • HHT Huntingtin protein
  • Cerebral ischemia is not a pathology with degenerative character; however, hitting the nervous cells that are destined to die can have an important effect also in pathological comorbidity with the other neurodegenerative pathologies, and it is present in epilepsies as well as in perinatal asphyxia and in cranial trauma. It is a highly-disabling disease which can be the cause of death or severe disabilities.
  • the ischemia occurs in an apparently sudden manner when a cerebral area is no longer supplied by the normal blood flow due to a heart attack, aneurism, vasal occlusions, hemorrhages, traumas etc.
  • the deprivation of oxygen and glucose during ischemia, and also the damage due to the blood reperfusion in the affected area lead the neuronal cells to undergo high metabolic stress.
  • the ischemia causes a reduction of ATP, with increase of intracellular calcium, oxidative damage and mitochondrial dysfunctions. All these events lead to a high glutamatergic transmission, which results excitotoxic and hence damaging for the neuronal cells that are destined to die.
  • MS Multiple sclerosis
  • the nervous cells transmit electric signals through the axons, which are covered by an isolating substance, the myelin sheath.
  • the pathogenetic mechanisms are complex and sustained by inflammatory and degeneratory processes on an excitotoxic basis; the exact etiology is still unknown.
  • the disease can manifest itself with a great array of neurological symptoms and can progress up to physical and cognitive disability.
  • Multiple sclerosis can assume various forms, including relapsing and progressive forms.
  • Major depressive disorder is a mood disorder or pathology, characterized by episodes of depressed mood accompanied by low self-esteem and loss of interest or pleasure in normally enjoyable activities.
  • Depression is a complex disease, due to genetic and environmental factors, which involves a complex network of neural systems.
  • the most confirmed hypothesis is the "monoaminergic theory of depression", according to which the clinical depression would derive from a reduced efficiency of one or more types of cerebral synapses which use monoamines, such as serotonin, dopamine or noradrenaline.
  • New studies have indicated the relation between depression and some valiants of a group of genes involved in the transmission of glutamate signals.
  • ketamine - a substance known for blocking the NMDA receptor - possesses antidepressive effects that are much quicker than those of conventional antidepressives, which instead require many weeks before showing results: ketamine is able to oppose depression in a much more effective manner than that which presently occurs with antidepressive drugs (which indeed do not effectively address the problem at present).
  • Schizophrenia is a severe mental disturbance, which is complex and often disabling.
  • the first signs of schizophrenia are generally manifested during adolescence and in early adult years.
  • the people affected by schizophrenia have difficulty in articulating their thoughts; this condition leads them to having hallucinations, deliria, incoherent thoughts as well as unusual behavior and speech. Due to these symptoms, the people hit by this disease have serious difficulties in interacting with others and tend to be isolated from the outside world.
  • One of the most classic hypotheses regards the role of dopamine: the malfunctioning of the dopaminergic neurons could be the cause of the symptoms that lead to the psychosis.
  • Recent results support the hypothesis of the anomalous relation between the systems of the dopamine and glutamate neurotransmitters in physical subjects, and suggests that the development of drugs capable of affecting the glutamate transmission pathway may be useful in treating psychoses.
  • Anxiety disorders are a common form of mental disease which often cause significant anguish and problems and lead to a reduced quality of life.
  • anxiety disorders for example, generalized anxiety disturbance, social anxiety disturbance, panic disturbance and obsessive-compulsive disturbance.
  • the cause of anxiety disorders is unknown. Nevertheless, some alterations of the cerebral functions appear involved in various anxietv disorders.
  • social and stress conditions could contribute to the development of anxiety disorders.
  • the comprehension of the biological bases involved with fear, anxiety and correlated disturbances -- even if still not fully complete - has seen considerable progress, due to the development of genetics, neurochemistry, psycopharmacoiogy and neuroimaging techniques.
  • a considerable increase regarding the knowledge of the neurobiological bases of anxiety is derived from the study of the behavioral components of the fear response, particularly related to the notions regarding the involved neuroanatomical pathways (amygdala, prefrontal cortex [PFC], thalamus and hippocampus), as well as the receptor and genetic aspects which can at least partly explain the different vulnerability of individuals to anxiety disorders.
  • the neurotransmitters involved an important role is played by GABA, glutamate, serotonin, neuropeptides and endocannabinoids.
  • Hemicrania is a highly-disabling neurological pathology limited to the central nervous system, which in the most severe cases is also associated with the symptoms of the autonomous nervous system. Typically the pathology is manifested with pain localized at the head, but is also associated with nausea, vomit, photophobia and phonophobia. Many of the people who suffer from hemicrania experience Aura, which is manifested in transitory visual, motor and sensory disturbances that precede cephalea. At present, the most confirmed molecular cause is the anomalous excitotoxic surge that occurs in the neurons, especially cortical, which is then followed by a depression of neuronal activity that is extended throughout the cortical area. The drugs presently used are the classic analgesic drugs associated with antiemetic drags in the most severe cases. Hence, also in this pathology, the excessive release of glutamate is at the basis of the organ disturbances.
  • Another pathology in which the release of glutamate is one of the causes of the pain symptoms is chronic neuropathic pain.
  • This is a pathology of the central nervous system that begins since the connection of the nervous signal between the peripheral fibers and the central fibers functions in an anomalous manner.
  • the symptoms are manifested with continuous burning sensations or electric shocks, and paresthesia are also often present around the zones of the primary pain location.
  • Hyperalgesia and aliodynia are a main characteristic of this pathology.
  • Neuropathic pain is unfortunately difficult to cure; the only drugs presently used are analgesic drags, which often result ineffective.
  • the present invention is based on the identification of a very specific mechanism of glutamate release induced by the activation of the NMDA receptor localized on the presynaptic compartment of the neuronal terminations, in particular, the present invention, described in detail hereinbelow, attains the identification of the involvement of the protein c- jun N -terminal Kinase (JNK) in the cascade of cellular events triggered by the activation of the NMDA receptor. This occurs while documenting - for the first time - the existence of the JNK proteins at the presynaptic level.
  • JNK protein c- jun N -terminal Kinase
  • JNKs are ubiquitous intracellular kinases with enzymatic activity associated with different signal pathways involved with many cellular functions.
  • JNK are proteins that cover a central role in the signals that lead to neuronal death, which is verified in various pathological conditions.
  • the JNKs can be activated following the stimulation of tyrosine kinase receptors, cytokine receptors, receptors coupled with G proteins and ligand-dependent ionic channels, including the NMDA receptor, and they represent a true indication for signaling cellular death. While JNK has been generally associated with the post-synaptic NMDA receptors, its presynaptic role remains largely undiscovered.
  • the present invention refers to a peptide system capable of specifically blocking the NMDA- induced presynaptic release of glutamate.
  • the present invention refers to two cell -permeable peptides suitably prepared for inhibiting the interaction of proteins involved in the control of the glutamate release. Upstream of the definition of the present peptide system, by means of biochemical and electrophysiological methods the presence of JNK at the presynaptic level was demonstrated, involved in the control of the NMDA-evoked release of glutamate.
  • Two cell-permeable peptides have therefore been designed which are capable of blocking this JNK2-STXla interaction.
  • the peptides were designed in a manner such that they can pass the cellular barriers and inhibit the interaction that lies at the presynaptic level. These two peptides are thus capable of specifically blocking the NMDA-induced presynaptic release of glutamate.
  • the two peptides will hereinbelow be termed JGRil and JGRi2.
  • FIGURE 1 shows the effect that the inhibition of the JNK protein has on the presynaptic release of glutamate, and it also shows the synaptic activation of the JNK protein under the action of different stimuli.
  • A) shows the results regarding the synaptosomes prepared from the cortex of Wild-type mice, which were pre-loaded with the radioactive neurotransmitter and then incubated with different stimuli as indicated. The results are expressed as percentage of the induced release. The data is expressed as mean ⁇ SEM (standard error of the mean) obtained from 3 experiments reproduced in triplicate (Three perfusion chambers for each experimental condition). **p ⁇ 0.01 vs. basal release.
  • C-D-E representative Western blots with relative quantifications that show the p-JNK level reached after 90 sec of stimulation of KC1 (B), 10 min of NMDA (C), 10 min of AMP A (D) and the treatment of the synaptosomes with L-JNKil .
  • the results are expressed as percentage of increase of the p-JNK/JNK ratio on the control (placed at 100%).
  • the data represents the mean ⁇ SEM of 4 experiments for each condition.
  • the treatment with NMDA induced an increase of the phosphorylation of JNK **p ⁇ 0.01 vs. 100% of the control) while JNKil does not reduce the effect of the NMDA (*p ⁇ 0.05 vs. 100% of the control).
  • FIGURE 2 shows the functional role of JNK in the release of glutamate dependent on NMDA.
  • A) depicts the histograms (mean + S.E.M) of the paired-pulse ratio (PPR) of evoked EPSCs that are registered by 7 neurons from slices of mouse of the strain C57BL6/J and by 10 neurons of slices of mouse C57BL6/J pre-incubated with L-JNKil (2 ⁇ ).
  • PPR paired-pulse ratio
  • B) shows the cumulative distribution of the amplitude of the mEPSC (left) and of the inter-event intervals (right) recorded from single neurons of C57BL6/J, or of neurons of C57BL6/J pre-incubated with L-JNKil in response to the D-AP5.
  • the top traces were obtained from the same neurons, in control conditions, during the administration of D-AP5 and subsequent washing.
  • C) depicts the histograms (mean + S.E.M) of the paired-pulse ratio (PPR) of evoked EPSCs which are recorded from 11 neurons of slices of mouse of the strain C57BL6/J and from 10 neurons of slices of mouse C57BL6/J pre-incubated with L-JNKil (2 ⁇ ) in response to the D-AP5. * *p ⁇ 0.01 (t- test) .
  • FIGURE 3 shows that the JNK controls the phosphorylation of Syntaxin l a and that JNK2/3 co-immunoprecipitates together with Syntaxin la.
  • A) shows the representative Western blot results with respective quantifications of the phosphorylation of STXla during the treatment as shown. The results are expressed as a percentage and are normalized with the control. The data is the mean ⁇ SEM of 5 experiments in which 10 min of NMD A + L-JNKil reduced the relation pSTXla/STXla (**p ⁇ 0.01 vs. 100% of the control) as did L-JNKil on its own (**p ⁇ 0.01 vs. 100% of the control).
  • B) shows the representative Western blot results with the respective quantifications that show the levels of the phosphorylation of JNK during the treatment with D-AP5. The results are expressed as percentage with respect to the control (100%). The data is the mean ⁇ SEM of 3 experiments.
  • C) shows the representative Western blot results with respective quantifications that show the levels of the phosphorylation of STXla during the treatment with D-AP5. The results are expressed as percentage and are normalized with the control. The data represents the mean + SEM of 3 experiments.
  • D) shows the results of the experiments of co- immunoprecipitation (Co-TP) with relative quantifications. Cortex synaptosomes of wild-type mice were stimulated as indicated in the figure and the immunoprecipitation executed with STXla antibody.
  • Representative Western blot results show Co-Ip of p-JNK and JNK after stripping procedure.
  • the membranes were blotted for STXla (as control of the immunoprecipitation) and for S YTl (as control of the specificity).
  • the results are expressed as percentage of increase of the relation p-JNK/STXla or JNK/STXla with respect to the control condition.
  • the data is the mean ⁇ SEM of 4 experiments for each treatment (*p ⁇ 0.05, **p ⁇ 0.01 vs. 100% of the control).
  • FIGURE 4 shows how the NMDA-evoked release of glutamate and the NMDA-induced phosphorylation of JNK are inhibited in the JNK2 KO mice.
  • A) shows the results of the glutamate release induced by NMD A stimulation in synaptosomes from the cortex of wild-type and JNK-KO mice pre-loaded with radioactive tracer. The results are expressed as a percentage of induced release.
  • the data is the mean ⁇ SEM of 4 experiments conducted in triplicate (3 perfusion chambers for each experimental condition). **p ⁇ 0.01 vs. WT; p ⁇ 0.05 vs. JNK1-KO or JNK3-KO.
  • B) shows the representative Western blot results with quantifications that show the ratio pJNK/JNK in the JNK2-KO or JNK3-KO mice during the treatments. The results are expressed as percentages with respect to the control (100%).
  • the data represents the mean + SEM of 3 experiments (**p ⁇ 0.01 vs. 100% of control).
  • FIGURE 5 shows how the isoform JNK2 is involved in the NMDA-mediated release of glutamate
  • A) represents the cumulative distribution of the amplitude of the mEPSC (left) and the inter-event intervals (right) recorded from single neurons of isoform JN KO mice in response to D-AP5 (50 ⁇ ).
  • the top lines are obtained from the same neurons, in control conditions, during the administration of D-AP5 and subsequent washing.
  • FIGURE 6 shows the model of molecular docking of the protein interaction JNK-STXla.
  • A,B,C represent the three best docking possibilities obtained from the study of the interaction between STXla and JNK2 respectively identified as models 1, 2 and 3.
  • the JNK2 protein is represented by the group 100, while the N-terminal portion of STXla is represented by the group 200.
  • D) shows the structure of the N-terminal portion of STXla: the sections 300, 400, and 500 are shown which represent the portions that, with highest probability, interact with JNK2. The rendering of all the images was carried out by means of the use of VMD software.
  • FIGURE 7 shows that the treatment with L-JNKil does not induce changes in the NMD A and AMP A receptor subunits (A), nor does it induce changes on the proteins of the release mechanism (B), neither during NMD A stimulation conditions nor if applied on its own.
  • FIGURE 8 shows the effect of the two peptides JGRil and JGRi2 on the release of glutamate in wild-type mouse cortex slices in electrophysiological patch-clamp experiments.
  • FIGURE 9 shows an example of a solid-phase chemical synthesis process for peptides. The symbols indicate: little circle, group of protection for the amino acid chain; R, protection for the amino group; X, Y, Z, L, functional groups.
  • the process is cyclically repeated until the peptide is attained.
  • FIGURE 10 shows the chromatogram obtained from the HPLC analysis of the peptide JGRil having sequence SEQ ID NO. 5: GRKKRRQRRRPPIEQS IEQEEGLNRS .
  • FIGURE 11 shows the chromatogram obtained from the HPLC analysis of the peptide JGRi2 having sequence SEQ ID NO. 8: GRKKRRQRRRPPMSEYNATQSDYRER.
  • FIGURE 12 shows the mass spectrum of the peptide JGRil having sequence SEQ ID NO. 5: GRKKRRQRRRPPIEQSIEQEEGLNRS .
  • FIGURE 13 shows the mass spectrum of the peptide JGRi2 having sequence SEQ ID NO. 8:
  • the present invention is based on the demonstration of the presence of the INK isoforms (JNKl, JNK2, JNK3) at the STXl a presynaptic level. Taking this protein interaction as the stalling point, two peptides were designed: cell-permeable JGRil and JGRi2, which are capable of blocking this JNK2- STXla interaction and reducing the release of glutamate induced by the stimulation of the NMDA receptor.
  • JNK Jun N-terminal Kinase
  • JNK kinases The physiological presence of JNK at the presynaptic level was demonstrated by executing the glutamate release experiments. Due to this experiment type, it was possible to study the role of the JNK kinases in the presynaptic compartment. Isolated cortical terminals (svnaptosomes) of mouse strain C57/BL6 were used. These svnaptosomes pre-loaded with tritiated D-aspartate ([ ' 1H ] D - Asp) were subjected to glutamate release induced by different stimuli.
  • the synaptosomes were stimulated with a method that has been used for years, namely superfusion, in which the synaptosomes are continuously washed by a continuous current solution, and at a certain point they are stimulated.
  • the tritiated glutamate that is liberated in this procedure is exclusively released due to presynaptic intracellular events and once collected is distributed into the beta-emitting radiation counters.
  • the transient exposure of the synapiosonies (90 s) to a slight depolaiization stimulation (8 iiiM KCl) induced a significant exocytotic release of [ ' ⁇ ] D - Asp (158 + 37 % vs. control.
  • L-JNKil a specific inhibitor which blocks the JNK signaling cascade, preventing JNK interaction with the target proteins that bind the JNK binding domain (JBD).
  • JBD JNK binding domain
  • paired -pulse facilitation PPF
  • EC entorhinal cortex
  • L-JNKil eliminates the probability of NMDA-dependent presynaptic release and is in line with the data of the release by synaptosomes, even in a more integral system like the cerebral slices. Subsequently, the effect of L- JNKil was studied on the NMDA-dependent presynaptic glutamate release by measuring the excitatory currents of miniature type (mEPSCs), according to a protocol that is already commonly used.
  • mEPSCs miniature type
  • Syntaxin la is an essential protein for the assembly of the SNARE complex (Soluble NSF Attachment Protein REceptor), which is an essential step for the release of neurotransmitter.
  • Soluble NSF Attachment Protein REceptor Soluble NSF Attachment Protein REceptor
  • the role of the phosphorylation of STXla in ser!4 (pSTXla) in the neurotransmitter release mechanism is still a controversial subject. Some have reported that the reduction of pSTXl a has as direct consequence the diminution of the neurotransmitter release, while others demonstrated that the diminution of pSTXla leads to facilitating the release of neurotransmitters .
  • the anti-synaptotagmin 1 antibody (Sytl) was also used, which is not immunoprecipitated, indicating that the interaction STXla - JNK2 / 3 is specific.
  • JNK2 modulates the N MDA-dependent release of glutamate in patch-clamp experiments
  • Electrophysiological recordings were carried out by recording the mEPSC, using the same above-described protocol from JNK1 KO, JNK2 - KO and JN K3 - KO mice.
  • the perfusion of D-AP5 induces an increase of the cumulative distribution of the inter-event intervals in JNKl - KO (*** p ⁇ 0.001, KS test, .I NK ! KO vs. D - APS, fig. 5A) and JNK3 KO (*** p ⁇ 0.001 , KS test, JNK3 - KO vs. D - APS, Fig.
  • D - APS produced no significant changes in mEPSC (p > 0.05, KS test, JNK2 -KO vs. D- P5; Fig 5A; p > 0.05, Student t-test, control vs. JNK2 KO, fig. 5B), or in amplitude i n > 0.05, KS test, JNK2-KO vs. D- P5, fig 5A; p > 0.05, Student t-test, control vs. JNK2 KO, fig. 5B).
  • this data suggests that only the JN K2 isoform mediates the presynaptic release of glutamate in our experimental conditions.
  • JNKl provides 18 characteristic residues, JNK2 45, while JNK3 only 11.
  • Characteristic residues are defined as those residues that are specific variants of only one isoform, being differentiated with respect to the other two JN K.
  • the characteristic residues are important in the protein -protein interactions, and specifically if Syntaxin la specifically interacts with only one variant of JNK, the characteristic residues of such variant should be different with respect to the other JNKs.
  • the fact that JNK2 is significantly different from JNK1 and JNK3 could be a first indication of its specific interaction with Syntaxin.
  • model 3 presents a surface area of 2680 A2.
  • the interface residues were then analyzed and compared with the characteristic residues of JNK2. This test demonstrated that in model 3, the N-terminal of Syntaxin la interacts with eight characteristic residues, in model 2 with five such residues and in model 1 with only two such residues.
  • a rendering image was then obtained that indicates the possible contact sites of the N- terminal portion of Syntaxin la (sections 300 and 400).
  • JNK JNK 'The inhibition of JNK does not induce changes in the machinery of the vesicular proteins and of the subunit levels of the NMD A or AMP A receptor.
  • Hb goes from amino acid 71 to 104
  • SEQ ID NO. 3 MSEYNATQSDYRER
  • the functionality of the peptides is based on the principle of interruption of the interaction of the JNK2 protein with the N -terminal portion of Syntaxin la.
  • these peptides will competitively participate in the interaction with JNK2, thus inhibiting the bond with endogenous Syntaxin la. This event is capable of reducing the release of glutamate.
  • the peptides that can be considered effective vary in length starting from the entire N-terminal portion of Syntaxin la, 288 amino acids, to a minimum of 10 amino acids corresponding to the sequence SEQ ID NO. 2: IEQEEGLNRS or of 14 amino acids corresponding to the sequence SEQ ID NO. 3: MSEYNATQSDYRER.
  • the sequence of the Tat protein (48-59) of the HIV virus was inserted before the N-terminal portion of the present sequences, hence adding another 12 amino acids.
  • Tat (48-59) has the following sequence of 12 amino acids: GRKKRRQRRRPP.
  • This sequence is very effective, whether it is added in N- or C- terminal position of each of the possible synthesized peptides.
  • One example of peptides are those synthesized in a manner so as to have a total length of 26 amino acids.
  • sequences of the two peptides are the following: JGRil(SEQ ID NO. 5): GRKKRRQRRRPPIEQSIEQEEGLNRS
  • JGRi2 (SEQ ID NO. 8): GRKKRRQRRRPPMSEYNATQSDYRER or (SEQ ID NO. 9) MSEYNATQSDYRERGRKKRRQRRRPP or even the respective mirrored sequences with regard to the tat sequence
  • the two peptides were synthesized with amino acids having L enantiomer structure, but also synthesized as D enantiomers in order to improve the stability thereof in a living organism.
  • the same sequences can be inserted, as corresponding DNA sequences, also in different vectors for expressions in cell lines, bacteria and also in vectors for virus production.
  • the insertion in the expression vectors can be of whole sequences, and hence as
  • JGRi2 (SEQ ID NO. 8): GRKKRRQRRRPPMSEYNATQSDYRER
  • small pieces of SNA may be added which encode small (tag) peptides, such as HA, GST, Myc, EGFP etc.
  • pGEX-4 for example: pGEX-4, pTAT, p-EGFP-Cl, pc-DNA, 3 generation lentiviral vectors, commercial or not also used for gene therapy, vectors for Sindbis virus, adenovirus for infection (transduction) of neurons.
  • the two peptides were tested in glutamate release experiments (as described above) and in electrophysiology experiments (patch clamp, as described above) in order to test their effectiveness in reducing the NMD-evoked release of neurotransmitter. Both peptides resulted active in decreasing the NMDA-dependent release of glutamate in patch-clamp experiments.
  • Mouse cortex slices were treated for 30 minutes before the recording of the cumulative distribution of the inter-events of the mEPSC with the peptides in various condition.
  • the two peptides (JGRil and JGRi2), whether added together at the concentration of 5 ⁇ or separately at the concentration of 10 ⁇ , reduced the functionality of the inhibitor D-AP5 in blocking the NMD A presynaptic receptor (fig. 8). This data indicates that the NMDA receptor is already effectively blocked by the two peptides in the intracellular pathway and hence the receptor inhibitor, which acts on the extracellular portion of NMDA, no longer has effect.
  • the two peptides demonstrated effectiveness both on the synaptosomes and on cerebral slices, indicating that, given that they were administered in the extracellular environment, they are capable of penetrating into the cells, also diffusing into integral tissue. It is also expected that the peptides are capable of being entirely diffused in an organism. In order to reduce their degradation, and hence increase their hemi-iife, the present peptide system was synthesized by using amino acids belonging to the D stereochemical series.
  • D-JGRil dG-dR-dK-d -dR-dR-dQ-dR-dR-dR-dP-dP-dl-dE-dQ-dS-dl-dE-dQ-dE-dE-dG- dL-dN--dR-dS
  • D-JGRi2 d-G-dR-dK-dK-dR-dR-dQ-dR-dR-dP-dP-dM-dS-dE-dY-dN-dA-dT-dQ-dS- dD-dY-dR-dE-dR.
  • the possible administration routes of these cell-permeable peptides comprise all the known combinations, which range from those with immediate bioavailability to those with release modified over time.
  • a new administration route is considered: the production of neuronal stem cells, engineered such that they produce the peptide system in cell-permeable form (expressing the sequence Tat) or non-cell-permeable form. These cells, once injected and once they have reached the cerebral zone hit by degenerative pathology, can proliferate and liberate the peptide system in situ.
  • the peptide chain is formed using amino acids in L stereochemical form, if the final peptide is in L form, as well as those in D form, if the final peptide is in D form.
  • the nucleotide sequence of the peptides - whether they all comprise the N-terminal portion of Syntaxin l a or even smaller peptides up to a length of 10 amino acids corresponding to the sequence (SEQ ID NO. 2) lEQEEGLNRS and of 14 amino acids corresponding to the sequence (SEQ ID NO. 3) MSEYNATQSDYRER and for all possible lengths, also with the addition at the front (N- terminal) or at the back (C- terminal) of the sequence Tat (SEQ ID NO. 4) GRK RRQRRRPP - are inserted in the plasmid expression vectors.
  • the nucleotide sequences are inserted corresponding to the amino acid sequences (SEQ ID NO. 2) lEQEEGLNRS, or (SEQ ID NO. 11) IEQSIEQEEGLNRS and (SEQ ID NO. 3) MSEYNATQSDYRER and also the entire N-terminal portion of Syntaxin la is inserted in the bacterial expression vector pTAT.
  • Plasmid DNA p-EGFP-Cl or pc- DNA
  • KCM 5X containing 0.5 M KCI; 0.15M CaC12; 0.25 M MgC12
  • 5 ⁇ MnC12 and H20 as needed up to 100 ⁇ of solution.
  • an equal volume of chemically competent DH5 alpha bacterial cells are added. They are left several minutes in ice (4°C). A thermal shock is caused by placing the entire solution at room temperature for several minutes.
  • a volume quantity is added equal to 600 ⁇ of Luria Broth medium (LB) (lOg/1 tryptone, 5g/l yeast extract, lOg/1 NaCl, 15g/l agar) heated at 37°C containing Ampiciliin 100 ,ug/ml, since the piasmids contain the resistance to this antibiotic. Then the solution is plated on agar with the same concentration of Ampiciliin. Then, proceed with the purification of the Plasmid DNA by means of MAXI-prep commercial kits, hence used for cellular transfections or for synthesis of lentivirai particles with classical methods from the literature.
  • LB Luria Broth medium
  • MAXI-prep commercial kits hence used for cellular transfections or for synthesis of lentivirai particles with classical methods from the literature.
  • the transformation of the bacteria BL21 and derivatives is carried out with a different protocol.
  • the bacterial growth will be tested so as to control the actual incorporation of the plasmid within the bacteria.
  • the grown colonies are extracted with sterile points and placed in 5 ml of LB with Ampiciliin. After a bacterial growth at 37°C for 5-6h, a miniprep is prepared with the commercial kits. Then, an aliquot of DNA solution obtained from each colony is subjected to enzymatic cutting, with restriction enzymes used for subcloning (37°C for 60 minutes).
  • the DNA is made to run on agarose gel and the cut sequence is controlled at the photometer.
  • Several ⁇ of the colony that expresses more plasmid is made to grow, in order to then prepare the glycerol stocks that are frozen at -80°C.
  • the bacteria coming from a single colony, are made to grow at 37°C under stirring (250rpm) as pre-inoculum in LB + Ampicillin (KX ⁇ g/ml).
  • 1/50 of the pre-inoculum is made to grow in fresh LB + Amp (the quantity depends on the intended size of the production) at 37°C (250 rpm) up to obtaining OD600 of 0.8 - 1.
  • the induction lasts 4-5h at 37°C at a speed of 250 rpm.
  • the bacteria are centrifuged at 8000 rpm for 20 minutes at 4°C.
  • the supernatant is decanted and the pellet is weighted and stored at -20°C.
  • a Lysis Buffer is added to the pellet with the following composition (O.IM TRIS-HCL ImM EDTA pH 7.5) with a proportion of 2ml of Lysis Buffer per gram of pellet.
  • the pellet is then resuspended and 1.5mg/g of Lisozyme is added (from a lOmg/ml stock) in order to lyse the bacteria.
  • the solution is incubated for 2h at room temperature and stirred at 200 rpm.
  • the lysate is incubated for 10 minutes on ice and then sonicated with 4 pulses each of 50 second duration. Between one pulse and the next, the solution is left for 1 minute in ice. Then, a centrifuge is executed at 12000 rpm for 20 minutes at 4°C.
  • the supernatant is stored and a flake of pellet is removed; such flake is broken up, making it soft.
  • Added to the lysate volume is a volume equal to 1/2 of Triton Buffer. Then, this is incubated for 30 minutes at room temperature with a stirring of 200 rpm. Centrifuge then for 20 minutes at 4°C at a speed of 12000 rpm. The pellet is resuspended in Lysis Buffer. Then, Triton buffer is added in a volume equal to half the volume that is in the tube. This is then incubated at room temperature at 200rpm. Centrifuge at 12000rpm for 20 minutes at 4°C.
  • the pellet is resuspended in washing buffer and centrituged at 12000 rpm for 20 minutes at 4°C. Wash the pellet again, another 3 or 4 times, always with washing buffer. The pellet is then stored at -20°C.
  • the pellet is solubilized with 5ml/g of solubilization buffer containing 6M Guanidinium and ImM DTT.
  • the supernatant is then dialyzed with 6M Guanidinium at pH 3-4.
  • the dialysis solution is changed 3 times: each washing is carried out after 3 hours and the third washing is kept the entire night at 4°C.
  • the dialysis solution is then changed with a sodium phosphate buffer ** 50 mM at pH 7 with the same mode used for the dialysis with guanidinium.
  • the rest of the dialyzed substance is purified on an ion-exchange column.
  • Cys Lys Gly Arg lie G n Arg G n Leu Gl u lie Th Gly Arg Thr Thr

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Abstract

La présente invention concerne un système de peptides comprenant au moins un peptide bloquant la libération présynaptique du glutamate, ledit peptide possédant la séquence SEQ ID NO. 5 : GRKKRRQRRRPPIEQSIEQEEGLNRS ; et/ou la séquence SEQ ID NO. 8 : GRKKRRQRRRPPMSEYNATQSDYRER étant destiné à être utilisé dans le traitement de pathologies associées à l'excitotoxicité du glutamate.
PCT/IB2015/051640 2014-04-04 2015-03-06 Système de peptides à perméabilité cellulaire permettant de traiter des maladies provoquées par l'excitotoxicité du glutamate WO2015150934A1 (fr)

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WO2014057484A1 (fr) * 2012-10-09 2014-04-17 Ramot At Tel-Aviv University Ltd. Peptides pour le traitement de maladies neurodégénératives

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