WO2015138960A3 - Ré-ingénierie moléculaire de l'équilibre excitation-inhibition dans des circuits mémoire - Google Patents

Ré-ingénierie moléculaire de l'équilibre excitation-inhibition dans des circuits mémoire Download PDF

Info

Publication number
WO2015138960A3
WO2015138960A3 PCT/US2015/020540 US2015020540W WO2015138960A3 WO 2015138960 A3 WO2015138960 A3 WO 2015138960A3 US 2015020540 W US2015020540 W US 2015020540W WO 2015138960 A3 WO2015138960 A3 WO 2015138960A3
Authority
WO
WIPO (PCT)
Prior art keywords
ablim3
memory
excitation
engineering
molecular
Prior art date
Application number
PCT/US2015/020540
Other languages
English (en)
Other versions
WO2015138960A2 (fr
Inventor
Amar SAHAY
Original Assignee
The General Hospital Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The General Hospital Corporation filed Critical The General Hospital Corporation
Priority to US15/125,796 priority Critical patent/US10287580B2/en
Publication of WO2015138960A2 publication Critical patent/WO2015138960A2/fr
Publication of WO2015138960A3 publication Critical patent/WO2015138960A3/fr
Priority to US16/389,467 priority patent/US20190241894A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/322—2'-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3233—Morpholino-type ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/352—Nature of the modification linked to the nucleic acid via a carbon atom
    • C12N2310/3521—Methyl
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/352—Nature of the modification linked to the nucleic acid via a carbon atom
    • C12N2310/3525—MOE, methoxyethoxy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/353—Nature of the modification linked to the nucleic acid via an atom other than carbon
    • C12N2310/3533—Halogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Rheumatology (AREA)
  • Public Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Veterinary Medicine (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des agents de régulation de la mémoire et des méthodes qui permettent de cibler l'élément 3 de la famille des protéines LIM de liaison à l'actine (ABLIM3).
PCT/US2015/020540 2014-03-14 2015-03-13 Ré-ingénierie moléculaire de l'équilibre excitation-inhibition dans des circuits mémoire WO2015138960A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US15/125,796 US10287580B2 (en) 2014-03-14 2015-03-13 Molecular re-engineering of excitation-inhibition balance in memory circuits
US16/389,467 US20190241894A1 (en) 2014-03-14 2019-04-19 Molecular re-engineering of excitation-inhibition balance in memory circuits

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461952934P 2014-03-14 2014-03-14
US61/952,934 2014-03-14

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US15/125,796 A-371-Of-International US10287580B2 (en) 2014-03-14 2015-03-13 Molecular re-engineering of excitation-inhibition balance in memory circuits
US16/389,467 Division US20190241894A1 (en) 2014-03-14 2019-04-19 Molecular re-engineering of excitation-inhibition balance in memory circuits

Publications (2)

Publication Number Publication Date
WO2015138960A2 WO2015138960A2 (fr) 2015-09-17
WO2015138960A3 true WO2015138960A3 (fr) 2015-11-19

Family

ID=54072601

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/020540 WO2015138960A2 (fr) 2014-03-14 2015-03-13 Ré-ingénierie moléculaire de l'équilibre excitation-inhibition dans des circuits mémoire

Country Status (2)

Country Link
US (2) US10287580B2 (fr)
WO (1) WO2015138960A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105861740B (zh) * 2016-06-24 2019-07-05 河北医科大学第四医院 Ablim3基因作为食管癌诊治标志物的用途
WO2023215805A1 (fr) * 2022-05-05 2023-11-09 Alnylam Pharmaceuticals, Inc. Compositions d'agent d'arni de protéine 3 lim se liant à l'actine (ablim3) et leurs procédés d'utilisation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110023143A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of neurodevelopmental genes in animals
WO2013185108A1 (fr) * 2012-06-07 2013-12-12 Massachusetts Institute Of Technology Méthodes et produits liés au cancer du poumon

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006113679A2 (fr) * 2005-04-15 2006-10-26 Board Of Regents, The University Of Texas System Administration d'arnsi par compositions lipidiques neutres
WO2011127222A1 (fr) * 2010-04-06 2011-10-13 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Diagnostic et traitement de mélanome par évaluation et modulation de nuak2

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110023143A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of neurodevelopmental genes in animals
WO2013185108A1 (fr) * 2012-06-07 2013-12-12 Massachusetts Institute Of Technology Méthodes et produits liés au cancer du poumon

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ALLY ET AL.: "Pattern Separation and Pattern Completion in Alzheimer's Disease: Evidence of Rapid Forgetting in Amnestic Mild Cognitive Impairment.", HIPPOCAMPUS., vol. 23, no. 12, 2013, pages 1246 - 1258, XP055237424, ISSN: 1050-9631 *
BARRIENTOS ET AL.: "Two Novel Members of the ABLIM Protein Family, ABLIM-2 and -3, Associate with STARS and Directly Bind F-actin.", J BIOL CHEM., vol. 282, no. 11, 2007, pages 8393 - 8403, XP055237422 *
CHOW ET AL.: "Serum response factor and myocardin mediate arterial hypercontractility and cerebral blood flow dysregulation in Alzheimer's phenotype.", PROC NATL ACAD SCI U S A., vol. 104, no. 3, 2007, pages 823 - 828, XP002592369, ISSN: 0027-8424 *
SAHAY ET AL.: "Pattem Separation: A Common Function for New Neurons in Hippocampus and Olfactory Bulb.", NEURON., vol. 70, no. 4, 2011, pages 582 - 588, XP028231885, ISSN: 0896-6273 *
WEINER ET AL.: "Military risk factors for Alzheimer's disease.", ALZHEIMERS DEMENT., vol. 9, no. 4, 2013, pages 445 - 451, XP055237425, ISSN: 1552-5260 *
YAMAMOTO ET AL.: "Therapeutic potential of inhibition of the NF-kB pathway in the treatment of inflammation and cancer.", J CLIN INVEST., vol. 107, no. 2, 2001, pages 135 - 142, XP002428353, ISSN: 0021-9738 *

Also Published As

Publication number Publication date
US20190241894A1 (en) 2019-08-08
US20160376588A1 (en) 2016-12-29
WO2015138960A2 (fr) 2015-09-17
US10287580B2 (en) 2019-05-14

Similar Documents

Publication Publication Date Title
WO2017040930A3 (fr) Biomarqueurs prédictifs du syndrome de libération de cytokines
WO2016130704A3 (fr) Procédés et compositions pour analyser des composants cellulaires
WO2015196128A3 (fr) Molécules d'acide nucléique alternatives et leurs utilisations
WO2015196130A3 (fr) Molécules d'acide nucléique alternatives et leurs utilisations
EA201892489A1 (ru) Число мишеней для мультиплексной оптимизированной амплификации с несоответствиями (moma)
WO2015191911A3 (fr) Microvésicules enrichies en protéines et leurs procédés de fabrication et d'utilisation
MY196882A (en) Recombinant binding proteins and their use
WO2015089511A3 (fr) Molécules d'acides nucléiques modifiés et leurs utilisations
EP3252172A3 (fr) Hybridation rapide pour l'enrichissement de cible de séquençage de nouvelle génération
WO2016065349A8 (fr) Arn non codants courts régulateurs de protéines (sprrna) et procédés d'utilisation
EP4219702A3 (fr) Variants d'enzymes et polynucléotides codant pour ces variants
WO2015149034A3 (fr) Fusions de gènes et variants de gènes associés au cancer
EP3964575A3 (fr) Variants d'alpha-amylase et polynucléotides codant pour ces derniers
WO2016109799A3 (fr) Procédé permettant d'isoler de l'arn à partir d'échantillons cliniques
WO2017053629A3 (fr) Insertion de gènes régulée par la lumière à l'aide de vecteurs viraux par l'intermédiaire de l'incorporation de protéines optogénétiques et l'insertion génétique de peptides contraints de manière non conformationnelle
EP3643706A4 (fr) Composé monomère d'acide nucléique modifié et analogue d'acide oligonucléique
EP3959342A4 (fr) Procédés et kits pour l'enrichissement et la détection de modifications d'adn et d'arn et de motifs fonctionnels
WO2015138960A3 (fr) Ré-ingénierie moléculaire de l'équilibre excitation-inhibition dans des circuits mémoire
WO2016154040A3 (fr) Étiquetage biosynthétique et séparation d'arn
WO2015150482A8 (fr) Produit de combinaison comprenant une structure d'acide nucléique et une molécule d'acide nucléique simple brin
WO2018231955A3 (fr) Isolement d'acides nucléiques cibles
WO2016145362A3 (fr) Inhibiteurs de protéine dek et procédés associés
EP3480309A4 (fr) Ensemble d'amorces, trousse et procédé de détection d'au moins deux acides nucléiques cibles
WO2014136124A3 (fr) Trousse d'essai de pcr en temps réel multiplexe pour la détection simultanée du virus de l'hépatite
WO2015170187A3 (fr) Procédés d'enrichissement perfectionnés

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15761521

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 15125796

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 15761521

Country of ref document: EP

Kind code of ref document: A2