WO2015129965A1 - Centrifugation kit - Google Patents

Centrifugation kit Download PDF

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Publication number
WO2015129965A1
WO2015129965A1 PCT/KR2014/005156 KR2014005156W WO2015129965A1 WO 2015129965 A1 WO2015129965 A1 WO 2015129965A1 KR 2014005156 W KR2014005156 W KR 2014005156W WO 2015129965 A1 WO2015129965 A1 WO 2015129965A1
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WO
WIPO (PCT)
Prior art keywords
mixed solution
centrifugation
container
outlet
chamber
Prior art date
Application number
PCT/KR2014/005156
Other languages
French (fr)
Korean (ko)
Inventor
장기영
Original Assignee
(주)어핀텍
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Publication date
Application filed by (주)어핀텍 filed Critical (주)어핀텍
Publication of WO2015129965A1 publication Critical patent/WO2015129965A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B04B7/12—Inserts, e.g. armouring plates
    • B04B7/16—Sieves or filters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/02—Centrifuges consisting of a plurality of separate bowls rotating round an axis situated between the bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes

Definitions

  • the present invention relates to a centrifugation kit, and more particularly, to a centrifugation kit that can easily extract or collect a desired final material through centrifugation by mixing a sample having a biological or chemical substance such as nucleic acid with a buffer. will be.
  • a centrifugation kit in general, includes a mixture containing a sample and a buffer for centrifugation in a state in which a spin column and a collection tube are combined, and the spin column is integrated with the spin column.
  • the vessel-coupled centrifuge kit is loaded into a rotor in the centrifuge so that the desired material in the mixed solution contained in the spin column by the centrifugal force of the centrifuge remains in the filter below the spin column and the remaining material and buffers are out of the spin column. Spilled and collected in an integrated container.
  • centrifugation kits of this kind require pretreatment before mixed materials such as samples and buffers are injected into the spin column.
  • lysis buffer and proteinase K are released to release DNA or RNA trapped in the cell wall.
  • lysis buffer and proteinase K are released to release DNA or RNA trapped in the cell wall.
  • the rotor used generally has an inlet of a 45 ° spin column assembly so that the liquid in the spin column or the centrifugal force after the centrifugal force does not flow down after the centrifugal force is applied, and the angle is used to prevent the liquid from flowing down.
  • Swing bucket rotor with angle rotor or swing bucket with spin column and integrated container to position the spin column and integrated container in the horizontal direction when centrifugal force is applied and to the vertical direction when there is no centrifugal force. (swing bucket rotor) is used.
  • the present inventors have developed a centrifugal kit having a pre-processing container which is directly connected to a spin column and directly inserted into a rotor of a centrifuge or a filter unit inserted with a pre-processing container. Reached.
  • the present invention has been made to solve the above problems, by providing a pre-treatment container or a pre-treatment container is inserted into the filter column is inserted into the rotor of the centrifuge directly connected to the spin column, the biological or chemical material for centrifugation It is an object of the present invention to provide a centrifugal separation kit that facilitates injection of a sample and a buffer, improves workability, and facilitates automation.
  • the present invention provides a chamber containing a mixed solution containing a sample and a buffer having a biological or chemical substance for centrifugation, an injection unit into which the sample and the buffer are injected into the chamber, and a centrifugation.
  • a pretreatment container having an outlet portion through which the mixed solution flows out of the chamber by a centrifugal force generated at the time;
  • An inlet part through which the mixed solution flowing out through the outlet part flows in, a mixed solution accommodating part accommodating the mixed solution introduced from the inlet part, and a discharge part discharging the centrifuged solution centrifuged from the mixed solution; It is provided between the inlet and the outlet provides a centrifugation kit comprising a spin column having a filter unit for filtering the mixed solution is centrifuged.
  • the apparatus may further include an integrated container having a centrifugal liquid accommodating part configured to receive the centrifugal liquid discharged through the filter part by being centrifuged from the spin column.
  • the outlet of the pretreatment container is characterized in that it is screwed, or fitted, or loosely inserted into the inlet of the spin column.
  • the accumulation container is located adjacent to the discharge portion, characterized in that provided inclined with respect to the axis of the discharge portion.
  • the object is a chamber containing a mixed solution containing a sample and a buffer having a biological or chemical substance for centrifugation, an injection unit into which the sample and the buffer is injected into the chamber, and the inlet and 45 °
  • a pretreatment container positioned at an angle of ⁇ 135 ° and having an outlet portion through which the mixed solution in the chamber is centrifuged by centrifugal force generated during centrifugation;
  • a filter unit provided at an outlet of the pretreatment container and filtering the mixed solution to be centrifuged; It is also achieved by a centrifugation kit comprising an integrated container formed with a centrifugation liquid receiving portion for receiving the centrifugal liquid discharged through the filter portion.
  • the integrated container is located adjacent to the outlet portion, characterized in that provided inclined with respect to the axis of the outlet portion.
  • the pretreatment container has four cylindrical side wall portions, an upper plate portion and a lower plate portion for blocking openings at both ends formed by the four side wall portions, and the injection portion does not leak the mixed solution in the chamber by centrifugal force generated during centrifugation. It is provided on the upper plate portion, and the outlet portion is characterized in that it is provided on the upper side of any one of the four side wall portion so that the mixed solution in the chamber smoothly flows out by the centrifugal force generated during centrifugation.
  • the injection portion and the outlet portion is characterized in that the angle of 45 ° to 135 °.
  • the width of the pretreatment container is increased from the injection portion toward the outlet portion.
  • the filter unit may be formed of a membrane or a media filter.
  • the pretreatment container is characterized in that it comprises a jaw to prevent the mixed solution contained in the chamber to flow out to the injection portion.
  • the present invention it is easy to inject the sample and the buffer by injecting the sample and the buffer having the biological or chemical substance for centrifugation through the pretreatment container provided at the front end of the spin column without directly injecting the sample into the spin column. It can improve the performance and facilitate the automation.
  • the filter unit may be embedded inside the outlet of the pretreatment container to simultaneously perform the roles of the pretreatment container and the spin column.
  • FIG. 1 is a perspective view of a centrifugal separation kit according to a first embodiment of the present invention
  • FIG. 2 is a cross-sectional view of FIG.
  • FIG. 3 is a perspective view of the pretreatment container of FIG.
  • FIG. 4 is a cross-sectional view of a centrifugal separation kit according to a second embodiment of the present invention.
  • FIG. 5 is a perspective view of the pretreatment container of FIG. 4, FIG.
  • FIG. 6 is a plan view of the rotor of the centrifuge in which the centrifugation kit according to the first and second embodiments of the present invention is used;
  • FIG. 7 is a longitudinal sectional view of FIG. 6;
  • FIG. 8 is a plan view showing a state in which the centrifuge kit according to the first and second embodiments of the present invention is mounted on the rotor of the centrifuge of FIG. 6;
  • FIG. 9 is a longitudinal sectional view of FIG. 8;
  • FIG. 10 is a cross-sectional view showing the centrifugation kit mounted on the rotor of the centrifuge according to the first and second embodiments of the present invention and centrifuged;
  • FIG. 11 is a perspective view of a centrifugal separation kit according to a third embodiment of the present invention.
  • FIG. 12 is a cross-sectional view of FIG. 11,
  • Figure 13 is a longitudinal cross-sectional view showing a state in which the centrifuge kit according to the third embodiment of the present invention is mounted on the rotor of the centrifuge,
  • FIG. 14 is a perspective view of a centrifugation kit according to a fourth embodiment of the present invention.
  • FIG. 15 is a perspective view of a centrifugal separation kit according to a fifth embodiment of the present invention.
  • FIG. 16 is a cross-sectional view of FIG. 15 schematically illustrating an outflow state of a mixed solution in a pretreatment container during centrifugation;
  • FIG. 17 is a perspective view of the pretreatment container of FIG. 15;
  • FIG. 18 is a front view of the pretreatment container of FIG. 15.
  • FIG. 1 and 2 illustrate a centrifugation kit according to a first embodiment of the present invention.
  • the centrifugation kit 10a includes a pretreatment container 11a and a spin column 31a.
  • the pre-processing container 11a is formed by four cylindrical side wall portions 13a, 13b, 13c, and 13d and four side wall portions 13a, 13b, 13c, and 13d.
  • a rectangular cylindrical shape having an upper plate portion 15 and a lower plate portion 17 for blocking openings formed at both ends is formed, and includes a chamber 21, an injection portion 23, and an outlet portion 25.
  • the chamber 21 is provided inside the pretreatment container 11a to accommodate a sample having a biological or chemical substance for centrifugation and a mixed solution 3 containing a buffer, and the mixture contained in the chamber 21 during centrifugation.
  • the solution 3 is centrifuged by centrifugal force.
  • the injection part 23 is provided in the upper plate part 15, and the sample and the buffer are injected into the chamber 21 through the injection part 23.
  • the injection part 23 is provided in the upper plate part 15 so that the mixed solution 3 in the chamber 21 does not flow out by the centrifugal force generated during centrifugation.
  • the outlet portion 25 is provided in the side wall portion 13a of any one of the four side wall portions 13a, 13b, 13c, and 13d, and the mixed solution 3 in the chamber 21 is caused by centrifugal force generated during centrifugation. Through the outlet part 25, it flows out of the pretreatment container 11a, ie toward the spin column 31a.
  • the outlet part 25 is provided above the side wall part 13a so that the mixed solution 3 in the chamber 21 does not flow out through the outlet part 25 when it is not centrifuged.
  • the outlet portion 25 has a stepped outer circumference of the outlet portion 25 so that the spin column 31a is fitted. Then, the outlet portion 25 forms a right angle with the injection portion 23.
  • the outlet portion 25 and the injection portion 23 may be provided at an angle of 45 ° to 135 °.
  • the pretreatment container 11a has a shape in which the width of the pretreatment container 11a increases from the injection portion 23 toward the outlet 25. This makes it possible to mount as many pretreatment containers 11a as possible in the rotor 101 (see FIG. 6) of the centrifuge.
  • the spin column 31a has a hollow circular tube shape of which both sides are open, and includes a mixed solution containing part 33, an inlet part 35, an outlet part 43, and a filter part 45.
  • the mixed solution accommodating part 33 is formed inside the spin column 31a and accommodates the mixed solution 3 introduced from the inlet part 35.
  • the mixed solution 3 contained in the mixed solution containing portion 33 is centrifuged by the centrifugal force generated during centrifugation.
  • the inlet part 35 is formed at an opening at one end of the spin column 31a to be fitted to the outlet part 25 of the pretreatment container 11a and to be mixed through the outlet part 25 of the pretreatment container 11a.
  • the solution 3 flows into the mixed solution containing portion 33 through the inlet portion 35.
  • the inlet portion 35 is equipped with a cap 39, the cap 39 is connected to the spin column 31a by a connecting string 41.
  • the cap 39 and the connecting strap 41 may be selectively interposed.
  • the discharge part 43 is formed at the other end of the spin column 31a, and the centrifugal liquid 5 (see FIG. 10) centrifuged from the mixed solution 3 is passed through the spin part 31a. ) Is discharged outward, that is, toward the accumulation vessel 51.
  • the discharge part 43 forms a step with the mixed solution accommodating part 33 and has a diameter relatively smaller than that of the injection part 23.
  • a filter portion 45 is provided at the lower end of the mixed solution containing portion 33 of the spin column 31a, that is, between the inlet portion 35 and the discharge portion 43 to filter the mixed solution 3 to be centrifuged. .
  • the filter part 45 selectively filters the mixed solution 3 discharged through the discharge part 43, that is, filters or passes a desired substance from the mixed solution 3 containing the sample and the buffer. do.
  • the filter part 45 may be formed of a membrane or a media filter. Common membrane components include silica, cellulose, nylon, and PTFE (polytetrafluorethylene). Exchange-exchange resins.
  • the centrifugation kit 10a according to the present invention further includes an integration container 51.
  • the accumulation container 51 has a predetermined length and has a hollow circular tube shape with one side opened. Inside the integrated container 51, a centrifugal liquid accommodating part 53 is formed which receives the centrifugal liquid 5 which is centrifuged from the spin column 31a and discharged through the filter part 45.
  • the accumulation vessel 51 is located adjacent to the discharge portion 43 of the spin column 31a and is provided to be inclined downward with respect to the axis of the discharge portion 43.
  • the integrated container 51 may be optionally interposed.
  • Figure 6 and Figure 7 shows the rotor of the centrifuge is centrifuged with a centrifuge kit according to the present invention.
  • the rotor 101 of the centrifuge has a disk shape, and a central hole 103 that can be coupled to a rotating shaft (not shown) of the motor is formed in the center, and the central hole 103 is formed.
  • the plurality of container seating portions 105, the plurality of column seating portions 107, and the plurality of container seating portions 111 are provided at equal intervals along the circumference of the central hole 103.
  • the pretreatment container 11a is seated on the container seating portion 105 so that the injection portion 23 of the pretreatment container 11a is positioned adjacent to the central hole 103.
  • a column support 109 on which the spin column 31a is supported is provided on the column seat 107 of the rotor 101 so that the spin column 31a connected to the pretreatment container 11a is horizontal.
  • the container support 113 is provided in the container mounting part 111 of the rotor 101 so that the integration container 51 may be inclined with respect to the axis of the spin column 31a and the discharge part 43. As shown in FIG.
  • a pair of pretreatment containers 11a, a pair of spin columns 31a, and a pair of integration vessels 51 are symmetrical and centrifuge The method of centrifuging in the state mounted on the rotor 101 is demonstrated.
  • the pretreatment container 11a, the spin column 31a, and the integrated container 51 are seated on the container seating portion 105, the column seating portion 107, and the container seating portion 111 of the rotor 101.
  • the spin column 31a is fitted to the outlet portion 25 of the pretreatment container 11a to connect the pretreatment container 11a and the spin column 31a.
  • a sample and a buffer having a biological or chemical substance such as nucleic acid for centrifugation are injected into the chamber 21 through the injection section 23 of the pretreatment container 11a, respectively.
  • the injection portion 23 of the pretreatment container 11a faces upward of the rotor 101, so that the sample and the buffer can be easily injected into the pretreatment container 11a using a pipette.
  • the mixed solution 3 including the sample and the buffer injected into the chamber 21 does not flow out through the outlet 25.
  • the rotor 101 of the centrifuge is rotated in one direction.
  • the mixed solution 3 contained in the pretreatment container 11a flows out through the outlet 25 by centrifugal force, and spins through the inlet 35 of the spin column 31a. It is accommodated in the mixed solution container 33 of the column 31a.
  • the mixed solution 3 contained in the mixed solution containing part 33 of the spin column 31a is centrifuged while being filtered through the filter part 45 by centrifugal force, followed by centrifuged centrifugal solution 5. Is discharged toward the integrated container 51 through the discharge portion 43 of the spin column 31a.
  • the male screw 27 is formed on the outer circumference of the outlet portion 25 of the pretreatment container 11b, the inlet portion of the spin column 31b ( A centrifugal separation kit 10b according to the second embodiment of the present invention is shown in which an internal thread 37 is formed at an inner circumference of 35 so that the pretreatment container 11b and the spin column 31b are screwed together.
  • the spin columns 31a and 31b are not directly injected into the spin columns 31a and 31b without the sample and the buffer having the biological or chemical substance for centrifugation.
  • the sample and the buffer can be easily injected, workability can be improved, and automation can be facilitated.
  • the outlet portion 25 of the pretreatment containers 11a and 11b and the inlet portion 35 of the spin columns 31a and 31b are screwed or fitted to the pretreatment containers 11a and 11b.
  • the spin columns 31a and 31b are described as being connected, although not shown, the outlet of the pretreatment container is loosely inserted into the inlet of the spin column, so that the mixed solution in the pretreatment container may flow into the spin column.
  • FIGS. 11 and 12 illustrate a centrifugal separation kit according to a third embodiment of the present invention.
  • the centrifugation kit 10c according to the third embodiment of the present invention unlike the above-described embodiments, does not have a spin column interposed between the pretreatment container 11c and the integration vessel 51. not.
  • the centrifugal separation kit 10c includes a pretreatment container 11c, a filter part 45, and an integration container 51.
  • the pretreatment container 11c of the centrifugal separation kit 10c according to the third embodiment of the present invention has the same shape and structure as the pretreatment container 11a according to the first embodiment described above. Omit the description. Meanwhile, the pretreatment container 11c of the centrifuge kit 10c according to the third embodiment of the present invention serves to centrifuge the mixed solution 3 in the chamber 21 by centrifugal force generated during centrifugation.
  • the filter part 45 is provided in the outflow part 25 of the pretreatment container 11c, and filters the mixed solution centrifuged.
  • the integrated container 51 has a hollow circular tube shape with one side opened, and the centrifugal liquid 5 centrifuged from the pretreatment container 11a inside the integrated container 51 and discharged through the filter part 45.
  • the centrifugal liquid accommodating part 53 which accommodates () is formed.
  • the accumulation container 51 is located adjacent to the outlet 25 of the pretreatment container 11a and is provided to be inclined with respect to the axis of the outlet 25.
  • the integrated container 51 has the same shape and structure as the integrated container according to the first embodiment described above, and a description thereof will be omitted below.
  • FIG. 13 is a longitudinal sectional view showing the centrifuge kit 10c according to the third embodiment of the present invention mounted on the rotor 101b of the centrifuge.
  • the rotor 101b of the centrifuge has a disk shape, and has a central hole 103 formed at the center thereof, which can be combined with a rotational axis (not shown) of the motor, and pretreatment in the outer radial direction about the central hole 103.
  • the container seating portion 105 and the container seating portion 111 in which the container 11c, the integrated container 51 are sequentially seated and disposed are formed.
  • the plurality of container seating portions 105 and the plurality of container seating portions 111 are provided at equal intervals along the circumference of the central hole 103.
  • the pretreatment container 11c is seated on the container seating portion 105 such that the injection portion 23 of the pretreatment container 11c is positioned adjacent to the central hole 103.
  • the container support 113 is provided in the container mounting part 111 of the rotor 101b so that the integration container 51 may be inclined with respect to the axis line of the outflow part 25 of the pretreatment container 11c.
  • the pretreatment container 11c and the integrated container 51 are seated on the container seating portion 105 and the container seating portion 111 of the rotor 101b, respectively, and then biological or chemical substances such as nucleic acids for centrifugation are removed.
  • the rotor 101b of the centrifuge is rotated in one direction, and the mixed solution contained in the pretreatment container 11c is carried out.
  • (3) is centrifuged while being filtered through the filter unit 45 by centrifugal force, and then the centrifuged centrifugal liquid 5 passes through the outlet 25 of the pretreatment container 11c. Is discharged towards.
  • the rotation of the rotor 101b of the centrifuge is stopped.
  • the desired centrifugal liquid 5 contained in the centrifugal liquid container 53 of the integrated container 51 can be obtained.
  • the sample and the buffer having the biological or chemical substance for centrifugation are injected through the injection unit 15 formed in the upper plate of the pretreatment container 11c, and the integrated container 51 is disposed at the rear end of the pretreatment container 11c.
  • the sample and the buffer is easy to inject, improve the workability, facilitate the automation as well as to reduce the overall length of the centrifugation kit (10c) compared to the above-described embodiments.
  • FIG. 14 illustrates a centrifugal separation kit 10d having a circular cross-sectional shape of a pretreatment container 11d as a centrifugal separation kit according to a fourth embodiment of the present invention.
  • centrifugal separation kit 10d differs only in shape from the pretreatment container 11c of the third embodiment, other configurations are the same, the description thereof will be omitted below.
  • the pretreatment container may have an elliptical cross-sectional shape in addition to the rectangular and circular cross-sectional shapes described above.
  • the mixed solution 3 contained in the chamber 21 is injected into the injection unit 23 unlike the first embodiment described above. It further comprises a stopper 29 for preventing the leakage.
  • the barrier rib 29 is recessed in a cross-sectional shape of a 'V' shape on the side wall portion 13c, for example, on the side wall portion 13c positioned opposite to the side wall portion 13a on which the outlet portion 25 is formed. It is.
  • the jaw 29 has the inlet 23 and the outlet 25 so that the mixed solution 3 in the chamber 21 flows smoothly toward the outlet 25 by the centrifugal force generated during centrifugation. Located in between.
  • the jaw 29 is described as being recessed in one side wall portion 13c in the present embodiment, the present invention is not limited thereto, and the jaw 29 protrudes in a cross-sectional shape of a 'V' shape inside the pretreatment container 11e. Or it may be formed to protrude in a band shape having a predetermined length and width.
  • the pretreatment container (11e) of the centrifugal separation kit (10e) is mutually with the fixing jaw 115 located below the rotor 101a (see Fig. 7) of the centrifuge during centrifugation. It further includes a protruding rib 27 protruding from the pretreatment container 11e so as to swing the pretreatment container 11e by the action.
  • the protruding ribs 27 in the present embodiment protrude from one side wall portion 13a of the pretreatment container 11e positioned in the direction opposite to the injection portion 23, but the present invention is not limited thereto. It may be formed to protrude from the lower plate portion 17.
  • the pretreatment container 11e is mounted on the rotor 101a of the centrifuge and the pretreatment container 11e rotates during centrifugation, so that the protruding ribs 27 rotate within the rotation radius of the pretreatment container 11e. It passes over a plurality of fixing jaw 115 of the centrifuge located protruded at intervals toward the pretreatment container (11e) at the lower side of the), the pretreatment container (11e) is rocked, the chamber 21 of the pretreatment container (11e) The mixed solution 3 accommodated in is stirred smoothly.
  • the pretreatment container 11e of the centrifugal separation kit 10e further includes a protruding rib 27 and a jaw 29 so that the centrifuge kit As the pretreatment container 11e rotates during separation, the protruding rib 27 passes over the fixing jaw 27 and the pretreatment container 11e swings, so that the mixed solution 3 contained in the chamber 21 of the pretreatment container 11e. This is stirred smoothly.
  • the mixed solution 3 in the chamber 21 does not flow out into the inlet 23 by the jaw 29, but smoothly flows out of the outlet 25.

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  • Centrifugal Separators (AREA)

Abstract

The present invention relates to a centrifugation kit that comprises a pre-treatment container and a spin column, wherein the pre-treatment container includes: a chamber for accommodating a mixed solution that contains a specimen having biological or chemical material for centrifugation and a buffer; an injection part through which the specimen and the buffer are injected into the chamber; and a discharge part through which the mixed solution in the chamber is discharged by a centrifugal force generated during the centrifugation, and the spin column includes: an introduction part through which the mixed solution discharged through the discharge part is introduced; a mixed-solution accommodating part for accommodating the mixed solution introduced from the introduction part; a drain part through which a centrifugal separation solution centrifugally separated from the mixed solution is drained; and a filter part disposed between the introduction part and the drain part to filter the mixed solution to be centrifugally separated. Accordingly, it is possible to facilitate the injection of a specimen and a buffer for centrifugation, enhance workability, and easily achieve automation.

Description

원심분리 키트Centrifuge Kit
본 발명은 원심분리 키트에 관한 것으로서, 보다 상세하게는, 핵산 등의 생물학적 또는 화학적 물질을 갖는 시료를 버퍼와 혼합하여 원심분리를 통해 원하는 최종 물질을 간편하게 추출하거나 채집할 수 있는 원심분리 키트에 관한 것이다.The present invention relates to a centrifugation kit, and more particularly, to a centrifugation kit that can easily extract or collect a desired final material through centrifugation by mixing a sample having a biological or chemical substance such as nucleic acid with a buffer. will be.
통상, 원심분리 키트는 스핀 컬럼(spin column)과 집적 용기(collection tube)가 결합된 상태에서 원심분리하기 위한 시료와 버퍼(buffer)가 함유된 혼합용액이 스핀 컬럼에 주입하고, 스핀 컬럼과 집적 용기가 결합된 원심분리 키트를 원심분리기 안의 로터(rotor) 안에 적재하여, 원심분리기의 원심력에 의해 스핀 컬럼에 수용된 혼합용액 내의 원하는 물질이 스핀 컬럼 하부의 필터에 남고 나머지 물질 및 버퍼들은 스핀 컬럼 밖으로 유출되어 집적 용기에 모이게 된다.In general, a centrifugation kit includes a mixture containing a sample and a buffer for centrifugation in a state in which a spin column and a collection tube are combined, and the spin column is integrated with the spin column. The vessel-coupled centrifuge kit is loaded into a rotor in the centrifuge so that the desired material in the mixed solution contained in the spin column by the centrifugal force of the centrifuge remains in the filter below the spin column and the remaining material and buffers are out of the spin column. Spilled and collected in an integrated container.
이러한 원리를 이용하여 여러 종류의 스핀 컬럼들이 공지되어 있고(U.S. Pat. No. 4,270,921(1981), U.S. Pat. No. 4,787,971(1988), U.S. Pat. No. 6,103,195(2000), U.S. Pat. No. 4,871,675(1989), U.S. Pat. No. 8,062,533(2011)),여러 제품들이 상업화(QIAamp DNA mini kits(Qiagen.com), Reliaprep™ Blood gDNA Mini prep System(Promega.com)) 되었다.Various types of spin columns are known using this principle (US Pat. No. 4,270,921 (1981), US Pat. No. 4,787,971 (1988), US Pat. No. 6,103,195 (2000), US Pat. 4,871,675 (1989), US Pat. No. 8,062,533 (2011), several products have been commercialized (QIAamp DNA mini kits (Qiagen.com), Reliaprep ™ Blood gDNA Mini prep System (Promega.com)).
이러한 종류의 상업화된 원심분리 키트는 시료와 버퍼 등의 혼합 물질이 스핀 컬럼에 주입되기 전에 전처리 과정이 필요하다.Commercialized centrifugation kits of this kind require pretreatment before mixed materials such as samples and buffers are injected into the spin column.
전처리 과정의 한 예로써 전혈(blood) 속의 DNA 또는 RNA 추출 시 세포벽에 갇혀 있는 DNA 또는 RNA 등을 방출하기 위해 라이시스 버퍼(lysis buffer), 그리고 프로틴에이스 K(proteinase K) 등을 시료와 외부 튜브에서 혼합한 후, 시료에 맞는 적절한 온도(37～80℃)에서 적절한 시간(5분에서 24시간)동안 히터 또는 인큐베이터에서 세포벽을 용해하는 라이시스(lysis) 작업을 시행하고 나서, 전처리 작업이 된 샘플을 스핀 컬럼 속에 페이펫으로 주입시킨 후, 스핀 컬럼을 집적 용기와 조립하고 나서, 원심분리기의 로터에 넣어서 스핀 컬럼을 사용한 추출 작업을 시작한다.As an example of the pretreatment process, when extracting DNA or RNA from blood, lysis buffer and proteinase K are released to release DNA or RNA trapped in the cell wall. After mixing at the appropriate temperature (37 ~ 80 ℃) for a suitable time (5 minutes to 24 hours) for lysing (lysis) to dissolve the cell wall in a heater or incubator, and then pre-treatment After the sample is injected into the spin column with a tapette, the spin column is assembled with the integration vessel and then placed in the rotor of the centrifuge to start the extraction operation using the spin column.
이 때, 사용되는 로터는 일반적으로 45°각도의 스핀 컬럼 조립체(spin column assembly)의 주입구가 있어 원심력이 작용하기 이전 스핀 컬럼 속의 액체 또는 원심력 작용한 후, 집적 용기 속의 액체가 흘러내리지 않게 하는 앵글 로터(angle rotor) 또는 스핀 컬럼과 집적 용기를 삽입한 스윙 버켓(swing bucket)을 사용하여 원심력이 작용할 때는 스핀 컬럼과 집적 용기가 수평방향으로 위치하고, 원심력이 없을 때는 수직방향으로 향하게 하는 스윙 버켓 로터(swing bucket rotor)를 사용한다.At this time, the rotor used generally has an inlet of a 45 ° spin column assembly so that the liquid in the spin column or the centrifugal force after the centrifugal force does not flow down after the centrifugal force is applied, and the angle is used to prevent the liquid from flowing down. Swing bucket rotor with angle rotor or swing bucket with spin column and integrated container to position the spin column and integrated container in the horizontal direction when centrifugal force is applied and to the vertical direction when there is no centrifugal force. (swing bucket rotor) is used.
이러한 외부 튜브에서 시행되는 전처리 작업의 불편성과, 전처리 후 샘플이 이동하면서 발생할 수 있는 오염성, 그리고 앵글 로터에 주입된 스핀 컬럼 속에 자동 피펫(pipett)을 사용하여 각도를 갖고 있는 스핀 컬럼의 내부에 액체를 주입하기 어렵고, 또한 스윙 버켓 로터의 회전시 버켓과 공기 마찰에 의해 고속 회전의 어려움으로 인해 자동화가 곤란한 문제점이 있다.Inconvenience of the pretreatment work performed on these outer tubes, contaminants that can occur as the sample moves after pretreatment, and liquid inside the spin column angled using an automatic pipett in the spin column injected into the angle rotor. It is difficult to inject, and also difficult to automate due to the difficulty of high-speed rotation by the bucket and air friction during rotation of the swing bucket rotor.
이에, 본 출원인은 이러한 문제점을 해결하기 위해, 스핀 컬럼과 바로 연결되어서 원심 분리기의 로터에 바로 삽입되는 전처리 컨테이너(pre-processing container) 또는 필터부가 삽입된 전처리 컨테이너를 갖는 원심분리 키트를 개발하기에 이르렀다.Therefore, in order to solve this problem, the present inventors have developed a centrifugal kit having a pre-processing container which is directly connected to a spin column and directly inserted into a rotor of a centrifuge or a filter unit inserted with a pre-processing container. Reached.
[선행기술문헌][Preceding technical literature]
[특허문헌][Patent Documents]
미국특허등록 US4,270,921US patent registration US4,270,921
미국특허등록 US6,103,195US patent registration US6,103,195
미국특허등록 US4,871,675US patent registration US4,871,675
미국특허등록 US8,062,533US patent registration US8,062,533
본 발명은 상기와 같은 문제점을 해결하기 위해 안출된 것으로, 스핀 컬럼과 연결되어서 원심 분리기의 로터에 바로 삽입되는 전처리 컨테이너 또는 필터부가 삽입된 전처리 컨테이너를 마련함으로써, 원심분리하기 위한 생물학적 또는 화학적 물질을 갖는 시료와 버퍼의 주입이 용이하고, 작업성을 향상시키며, 자동화를 용이하게 하는 원심분리 키트를 제공하는 것을 목적으로 한다.The present invention has been made to solve the above problems, by providing a pre-treatment container or a pre-treatment container is inserted into the filter column is inserted into the rotor of the centrifuge directly connected to the spin column, the biological or chemical material for centrifugation It is an object of the present invention to provide a centrifugal separation kit that facilitates injection of a sample and a buffer, improves workability, and facilitates automation.
본 발명은 상기 목적을 달성하기 위해, 원심분리하기 위한 생물학적 또는 화학적 물질을 갖는 시료와 버퍼가 함유된 혼합용액을 수용하는 챔버와, 상기 챔버로 상기 시료와 버퍼가 주입되는 주입부와, 원심분리시 발생하는 원심력에 의해 상기 챔버 내의 상기 혼합용액이 유출되는 유출부를 갖는 전처리 컨테이너와; 상기 유출부를 통해 유출되는 상기 혼합용액이 유입되는 유입부와, 상기 유입부로부터 유입된 상기 혼합용액을 수용하는 혼합용액 수용부와, 상기 혼합용액으로부터 원심분리된 원심분리액이 배출되는 배출부와, 상기 유입부와 상기 배출부 사이에 마련되어 원심분리되는 상기 혼합용액을 여과하는 필터부를 갖는 스핀 컬럼을 포함하는 것을 특징으로 하는 원심분리 키트를 제공한다.In order to achieve the above object, the present invention provides a chamber containing a mixed solution containing a sample and a buffer having a biological or chemical substance for centrifugation, an injection unit into which the sample and the buffer are injected into the chamber, and a centrifugation. A pretreatment container having an outlet portion through which the mixed solution flows out of the chamber by a centrifugal force generated at the time; An inlet part through which the mixed solution flowing out through the outlet part flows in, a mixed solution accommodating part accommodating the mixed solution introduced from the inlet part, and a discharge part discharging the centrifuged solution centrifuged from the mixed solution; It is provided between the inlet and the outlet provides a centrifugation kit comprising a spin column having a filter unit for filtering the mixed solution is centrifuged.
여기서, 상기 스핀 컬럼으로부터 원심분리되어 상기 필터부를 거쳐 배출된 원심분리액을 수용하는 원심분리액 수용부가 형성된 집적 용기를 더 포함할 수 있다.The apparatus may further include an integrated container having a centrifugal liquid accommodating part configured to receive the centrifugal liquid discharged through the filter part by being centrifuged from the spin column.
상기 전처리 컨테이너의 유출부는 상기 스핀 컬럼의 유입부에 나사 결합, 또는 끼움 결합, 또는 느슨하게 삽입되는 것을 특징으로 한다.The outlet of the pretreatment container is characterized in that it is screwed, or fitted, or loosely inserted into the inlet of the spin column.
상기 집적 용기는 상기 배출부에 인접하게 위치하며, 상기 배출부의 축선에 대해 경사지게 마련되는 것을 특징으로 한다.The accumulation container is located adjacent to the discharge portion, characterized in that provided inclined with respect to the axis of the discharge portion.
또한, 상기 목적은, 원심분리하기 위한 생물학적 또는 화학적 물질을 갖는 시료와 버퍼가 함유된 혼합용액을 수용하는 챔버와, 상기 챔버로 상기 시료와 버퍼가 주입되는 주입부와, 상기 주입부와 45°～135°의 각도를 이루면서 위치하여 원심분리시 발생하는 원심력에 의해 원심분리되는 상기 챔버 내의 상기 혼합용액이 유출되는 유출부를 갖는 전처리 컨테이너와; 상기 전처리 컨테이너의 유출부에 마련되어, 원심분리되는 상기 혼합용액을 여과하는 필터부와; 상기 필터부를 거쳐 배출된 원심분리액을 수용하는 원심분리액 수용부가 형성된 집적 용기를 포함하는 것을 특징으로 하는 원심분리 키트에 의해서도 달성된다.In addition, the object is a chamber containing a mixed solution containing a sample and a buffer having a biological or chemical substance for centrifugation, an injection unit into which the sample and the buffer is injected into the chamber, and the inlet and 45 ° A pretreatment container positioned at an angle of ˜135 ° and having an outlet portion through which the mixed solution in the chamber is centrifuged by centrifugal force generated during centrifugation; A filter unit provided at an outlet of the pretreatment container and filtering the mixed solution to be centrifuged; It is also achieved by a centrifugation kit comprising an integrated container formed with a centrifugation liquid receiving portion for receiving the centrifugal liquid discharged through the filter portion.
여기서, 상기 집적 용기는 상기 유출부에 인접하게 위치하며, 상기 유출부의 축선에 대해 경사지게 마련되는 것을 특징으로 한다.Here, the integrated container is located adjacent to the outlet portion, characterized in that provided inclined with respect to the axis of the outlet portion.
상기 전처리 컨테이너는 통형상의 네 측벽부와, 상기 네 측벽부에 의해 형성된 양단 개구를 차단하는 상판부 및 하판부를 가지며, 상기 주입부는 원심분리시 발생하는 원심력에 의해 상기 챔버 내의 상기 혼합용액이 유출되지 않도록 상기 상판부에 마련되고, 상기 유출부는 원심분리시 발생하는 원심력에 의해 상기 챔버 내의 상기 혼합용액이 원활하게 유출되도록 상기 네 측벽부 중 어느 하나의 측벽부의 상측에 마련되는 것을 특징으로 한다.The pretreatment container has four cylindrical side wall portions, an upper plate portion and a lower plate portion for blocking openings at both ends formed by the four side wall portions, and the injection portion does not leak the mixed solution in the chamber by centrifugal force generated during centrifugation. It is provided on the upper plate portion, and the outlet portion is characterized in that it is provided on the upper side of any one of the four side wall portion so that the mixed solution in the chamber smoothly flows out by the centrifugal force generated during centrifugation.
상기 주입부와 상기 유출부는 45°∼135°의 각도를 이루는 것을 특징으로 한다.The injection portion and the outlet portion is characterized in that the angle of 45 ° to 135 °.
상기 주입부로부터 상기 유출부를 향할수록 상기 전처리 컨테이너의 폭은 증대하는 것을 특징으로 한다.The width of the pretreatment container is increased from the injection portion toward the outlet portion.
상기 필터부는 분리막(membrane) 또는 여과제(media filter)로 이루어지는 것을 특징으로 한다.The filter unit may be formed of a membrane or a media filter.
상기 전처리 컨테이너는 상기 챔버에 수용된 혼합용액이 상기 주입부로 유출되는 것을 방지하는 방지턱을 포함하는 것을 특징으로 한다.The pretreatment container is characterized in that it comprises a jaw to prevent the mixed solution contained in the chamber to flow out to the injection portion.
원심분리시 원심분리기의 로터의 하측에 위치한 고정턱과의 상호 작용에 의해 상기 전처리 컨테이너가 요동하도록 상기 전처리 컨테이너에 돌출 형성된 돌출 리브를 더 포함하는 것을 특징으로 한다.It characterized in that it further comprises a protruding rib protruding in the pre-treatment container to swing the pre-treatment container by interaction with the fixing jaw located below the rotor of the centrifuge during centrifugation.
본 발명에 따르면, 원심분리하기 위한 생물학적 또는 화학적 물질을 갖는 시료와 버퍼를 스핀 컬럼에 직접 주입하지 않고, 스핀 컬럼의 전단에 마련된 전처리 컨테이너를 통해 주입함으로써, 시료와 버퍼의 주입이 용이하고, 작업성을 향상시키며, 자동화를 용이하게 할 수 있다.According to the present invention, it is easy to inject the sample and the buffer by injecting the sample and the buffer having the biological or chemical substance for centrifugation through the pretreatment container provided at the front end of the spin column without directly injecting the sample into the spin column. It can improve the performance and facilitate the automation.
또한 전처리 컨테이너의 배출부 안쪽에 필터부를 내장시켜 전처리 컨테이너와 스핀 컬럼의 역할을 동시에 수행하게 할 수 있다.In addition, the filter unit may be embedded inside the outlet of the pretreatment container to simultaneously perform the roles of the pretreatment container and the spin column.
도 1은 본 발명의 제1실시예에 따른 원심분리 키트의 사시도,1 is a perspective view of a centrifugal separation kit according to a first embodiment of the present invention;
도 2는 도 1의 단면도,2 is a cross-sectional view of FIG.
도 3은 도 1의 전처리 컨테이너의 사시도,3 is a perspective view of the pretreatment container of FIG.
도 4는 본 발명의 제2실시예에 따른 원심분리 키트의 단면도,4 is a cross-sectional view of a centrifugal separation kit according to a second embodiment of the present invention;
도 5는 도 4의 전처리 컨테이너의 사시도,5 is a perspective view of the pretreatment container of FIG. 4, FIG.
도 6은 본 발명의 제1 및 제2실시예에 따른 원심분리 키트가 사용되는 원심분리기의 로터의 평면도,6 is a plan view of the rotor of the centrifuge in which the centrifugation kit according to the first and second embodiments of the present invention is used;
도 7은 도 6의 종단면도,7 is a longitudinal sectional view of FIG. 6;
도 8은 도 6의 원심분리기의 로터에 본 발명의 제1 및 제2실시예에 따른 원심분리 키트가 장착된 모습을 도시한 평면도,8 is a plan view showing a state in which the centrifuge kit according to the first and second embodiments of the present invention is mounted on the rotor of the centrifuge of FIG. 6;
도 9는 도 8의 종단면도,9 is a longitudinal sectional view of FIG. 8;
도 10은 본 발명의 제1 및 제2실시예에 따른 원심분리 키트가 원심분리기의 로터에 장착되어 원심분리되는 모습을 도시한 단면도,10 is a cross-sectional view showing the centrifugation kit mounted on the rotor of the centrifuge according to the first and second embodiments of the present invention and centrifuged;
도 11은 본 발명의 제3실시예에 따른 원심분리 키트의 사시도,11 is a perspective view of a centrifugal separation kit according to a third embodiment of the present invention;
도 12는 도 11의 단면도,12 is a cross-sectional view of FIG. 11,
도 13은 본 발명의 제3실시예에 따른 원심분리 키트가 원심분리기의 로터에 장착된 모습을 도시한 종단면도,Figure 13 is a longitudinal cross-sectional view showing a state in which the centrifuge kit according to the third embodiment of the present invention is mounted on the rotor of the centrifuge,
도 14는 본 발명의 제4실시예에 따른 원심분리 키트의 사시도,14 is a perspective view of a centrifugation kit according to a fourth embodiment of the present invention;
도 15는 본 발명의 제5실시예에 따른 원심분리 키트의 사시도,15 is a perspective view of a centrifugal separation kit according to a fifth embodiment of the present invention;
도 16은 원심분리시 전처리 컨테이너에서의 혼합용액의 유출 상태를 개략적으로 도시한 도 15의 단면도,FIG. 16 is a cross-sectional view of FIG. 15 schematically illustrating an outflow state of a mixed solution in a pretreatment container during centrifugation;
도 17은 도 15의 전처리 컨테이너의 사시도,17 is a perspective view of the pretreatment container of FIG. 15;
도 18은 도 15의 전처리 컨테이너의 정면도이다.18 is a front view of the pretreatment container of FIG. 15.
이하, 첨부도면을 참조하여 본 발명에 대해 상세히 설명한다.Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.
설명에 앞서, 여러 실시예에 있어서, 동일한 구성을 가지는 구성요소에 대해서는 동일 부호를 사용하여 대표적으로 제1실시예에서 설명하고, 그 외의 실시예에서는 제1실시예와 다른 구성에 대해서만 설명하기로 한다.Prior to the description, in various embodiments, components having the same configuration will be representatively described in the first embodiment using the same reference numerals, and in other embodiments, only the configuration different from the first embodiment will be described. do.
도 1 및 도 2에는 본 발명의 제1실시예에 따른 원심분리 키트가 도시되어 있다.1 and 2 illustrate a centrifugation kit according to a first embodiment of the present invention.
이들 도면에 도시된 바와 같이, 본 발명의 제1실시예에 따른 원심분리 키트(10a)는 전처리 컨테이너(11a)와 스핀 컬럼(31a, spin column)을 포함한다.As shown in these figures, the centrifugation kit 10a according to the first embodiment of the present invention includes a pretreatment container 11a and a spin column 31a.
전처리 컨테이너(11a, pre-processing container)는 도 3에 도시된 바와 같이, 통형상의 네 측벽부(13a,13b,13c,13d)와, 네 측벽부(13a,13b,13c,13d)에 의해 형성된 양단 개구를 차단하는 상판부(15) 및 하판부(17)를 갖는 사각통형상을 이루며, 챔버(21)와 주입부(23)와 유출부(25)를 포함한다.As shown in FIG. 3, the pre-processing container 11a is formed by four cylindrical side wall portions 13a, 13b, 13c, and 13d and four side wall portions 13a, 13b, 13c, and 13d. A rectangular cylindrical shape having an upper plate portion 15 and a lower plate portion 17 for blocking openings formed at both ends is formed, and includes a chamber 21, an injection portion 23, and an outlet portion 25.
챔버(21)는 전처리 컨테이너(11a)의 내측에 마련되어, 원심분리하기 위한 생물학적 또는 화학적 물질을 갖는 시료와 버퍼가 함유된 혼합용액(3)을 수용하며, 원심분리시 챔버(21)에 수용된 혼합용액(3)은 원심력에 의해 원심분리된다.The chamber 21 is provided inside the pretreatment container 11a to accommodate a sample having a biological or chemical substance for centrifugation and a mixed solution 3 containing a buffer, and the mixture contained in the chamber 21 during centrifugation. The solution 3 is centrifuged by centrifugal force.
주입부(23)는 상판부(15)에 마련되며, 시료와 버퍼는 주입부(23)를 통해 챔버(21)로 주입된다. 주입부(23)는 원심분리시 발생하는 원심력에 의해 챔버(21) 내의 혼합용액(3)이 유출되지 않도록 상판부(15)에 마련된다.The injection part 23 is provided in the upper plate part 15, and the sample and the buffer are injected into the chamber 21 through the injection part 23. The injection part 23 is provided in the upper plate part 15 so that the mixed solution 3 in the chamber 21 does not flow out by the centrifugal force generated during centrifugation.
유출부(25)는 네 측벽부(13a,13b,13c,13d) 중 어느 하나의 측벽부(13a)에 마련되며, 챔버(21) 내의 혼합용액(3)은 원심분리시 발생하는 원심력에 의해 유출부(25)를 통해 전처리 컨테이너(11a)의 외측으로, 즉 스핀 컬럼(31a)을 향해 유출된다. 또한, 유출부(25)는 원심분리하지 않을 때 챔버(21) 내의 혼합용액(3)이 유출부(25)를 통해 유출하지 않도록 측벽부(13a)의 상측에 마련된다. 유출부(25)는 스핀 컬럼(31a)이 끼움 결합되도록 유출부(25)의 외주는 단차가 형성되어 있다. 그리고, 유출부(25)는 주입부(23)와 직각을 이룬다. 여기서, 유출부(25)와 주입부(23)는 45°∼135°의 각도를 이루며 마련될 수 있다.The outlet portion 25 is provided in the side wall portion 13a of any one of the four side wall portions 13a, 13b, 13c, and 13d, and the mixed solution 3 in the chamber 21 is caused by centrifugal force generated during centrifugation. Through the outlet part 25, it flows out of the pretreatment container 11a, ie toward the spin column 31a. In addition, the outlet part 25 is provided above the side wall part 13a so that the mixed solution 3 in the chamber 21 does not flow out through the outlet part 25 when it is not centrifuged. The outlet portion 25 has a stepped outer circumference of the outlet portion 25 so that the spin column 31a is fitted. Then, the outlet portion 25 forms a right angle with the injection portion 23. Here, the outlet portion 25 and the injection portion 23 may be provided at an angle of 45 ° to 135 °.
한편, 전처리 컨테이너(11a)는 주입부(23)로부터 유출부(25)를 향할수록 전처리 컨테이너(11a)의 폭이 증대하는 형상을 가진다. 이로써, 원심분리기의 로터(101, 도 6참조)에 가능한 많은 전처리 컨테이너(11a)를 안착할 수 있게 된다.On the other hand, the pretreatment container 11a has a shape in which the width of the pretreatment container 11a increases from the injection portion 23 toward the outlet 25. This makes it possible to mount as many pretreatment containers 11a as possible in the rotor 101 (see FIG. 6) of the centrifuge.
스핀 컬럼(31a)은 양측이 개구된 속이 빈 원형의 튜브 형상을 가지며, 혼합용액 수용부(33)와 유입부(35)와 배출부(43)와 필터부(45)를 포함한다.The spin column 31a has a hollow circular tube shape of which both sides are open, and includes a mixed solution containing part 33, an inlet part 35, an outlet part 43, and a filter part 45.
혼합용액 수용부(33)는 스핀 컬럼(31a)의 내측에 형성되며, 유입부(35)로부터 유입된 혼합용액(3)을 수용한다. 혼합용액 수용부(33)에 수용된 혼합용액(3)은 원심분리시 발생하는 원심력에 의해 원심분리된다.The mixed solution accommodating part 33 is formed inside the spin column 31a and accommodates the mixed solution 3 introduced from the inlet part 35. The mixed solution 3 contained in the mixed solution containing portion 33 is centrifuged by the centrifugal force generated during centrifugation.
유입부(35)는 스핀 컬럼(31a)의 일측 단부에 개구 형성되어 전처리 컨테이너(11a)의 유출부(25)에 끼움 결합되며, 전처리 컨테이너(11a)의 유출부(25)를 거쳐 유출되는 혼합용액(3)은 유입부(35)를 통해 혼합용액 수용부(33)로 유입된다. 한편, 유입부(35)에는 캡(39)이 장착되며, 캡(39)은 연결끈(41)에 의해 스핀 컬럼(31a)에 연결되어 있다. 여기서, 캡(39)과 연결끈(41)은 선택적으로 개재될 수 있다.The inlet part 35 is formed at an opening at one end of the spin column 31a to be fitted to the outlet part 25 of the pretreatment container 11a and to be mixed through the outlet part 25 of the pretreatment container 11a. The solution 3 flows into the mixed solution containing portion 33 through the inlet portion 35. On the other hand, the inlet portion 35 is equipped with a cap 39, the cap 39 is connected to the spin column 31a by a connecting string 41. Here, the cap 39 and the connecting strap 41 may be selectively interposed.
배출부(43)는 스핀 컬럼(31a)의 타측 단부에 개구 형성되며, 혼합용액(3)으로부터 원심분리된 원심분리액(5, 도 10참조)은 배출부(43)를 통해 스핀 컬럼(31a)의 외측으로, 즉 집적 용기(51)를 향해 배출된다. 배출부(43)는 혼합용액 수용부(33)와 단차를 형성하며, 주입부(23)보다 상대적으로 작은 직경을 가진다.The discharge part 43 is formed at the other end of the spin column 31a, and the centrifugal liquid 5 (see FIG. 10) centrifuged from the mixed solution 3 is passed through the spin part 31a. ) Is discharged outward, that is, toward the accumulation vessel 51. The discharge part 43 forms a step with the mixed solution accommodating part 33 and has a diameter relatively smaller than that of the injection part 23.
스핀 컬럼(31a)의 혼합용액 수용부(33)의 하단에는, 즉 유입부(35)와 배출부(43) 사이에는 필터부(45)가 마련되어, 원심분리되는 혼합용액(3)을 여과한다. 필터부(45)는 배출부(43)를 통해 배출되는 혼합용액(3)을 선택적으로 여과하는, 즉 시료와 버퍼가 함유된 혼합용액(3)으로부터 원하는 물질을 걸러 내거나, 또는 통과시키는 역할을 한다. 필터부(45)는 분리막(Membrane) 또는 여과제(Media filter)로 이루어 질 수 있다. 일반적인 분리막의 구성물질은 실리카(Silica), 셀루로스(Cellulose), 나이론(Nylon), 그리고 PTFE(Polytetrafluorethylene) 등이며, 여과제의 구성 물질은 덱스트린(Dextrans), 폴리아키릴아미드(Polyacryamides), 그리고 이온 익스체인지 레진(ion-exchange resin) 등이다.A filter portion 45 is provided at the lower end of the mixed solution containing portion 33 of the spin column 31a, that is, between the inlet portion 35 and the discharge portion 43 to filter the mixed solution 3 to be centrifuged. . The filter part 45 selectively filters the mixed solution 3 discharged through the discharge part 43, that is, filters or passes a desired substance from the mixed solution 3 containing the sample and the buffer. do. The filter part 45 may be formed of a membrane or a media filter. Common membrane components include silica, cellulose, nylon, and PTFE (polytetrafluorethylene). Exchange-exchange resins.
한편, 본 발명에 따른 원심분리 키트(10a)는 집적 용기(51, collection container)를 더 포함한다.On the other hand, the centrifugation kit 10a according to the present invention further includes an integration container 51.
집적 용기(51)는 일정 길이를 가지며, 일측이 개구된 속이 빈 원형의 튜브 형상을 가진다. 집적 용기(51)의 내측에는 스핀 컬럼(31a)으로부터 원심분리되어 필터부(45)를 거쳐 배출된 원심분리액(5)을 수용하는 원심분리액 수용부(53)가 형성되어 있다. 집적 용기(51)는 스핀 컬럼(31a)의 배출부(43)에 인접하게 위치하며, 배출부(43)의 축선에 대해 하향 경사지게 마련된다. 여기서, 집적 용기(51)는 선택적으로 개재될 수 있다.The accumulation container 51 has a predetermined length and has a hollow circular tube shape with one side opened. Inside the integrated container 51, a centrifugal liquid accommodating part 53 is formed which receives the centrifugal liquid 5 which is centrifuged from the spin column 31a and discharged through the filter part 45. The accumulation vessel 51 is located adjacent to the discharge portion 43 of the spin column 31a and is provided to be inclined downward with respect to the axis of the discharge portion 43. Here, the integrated container 51 may be optionally interposed.
한편, 도 6 및 도 7에는 본 발명에 따른 원심분리 키트가 장착되어 원심분리되는 원심분리기의 로터가 도시되어 있다.On the other hand, Figure 6 and Figure 7 shows the rotor of the centrifuge is centrifuged with a centrifuge kit according to the present invention.
이들 도면에 도시된 바와 같이, 원심분리기의 로터(101)는 원반 형상을 가지며, 중앙에 모터의 회전축(미도시)과 결합될 수 있는 중앙 홀(103)이 형성되어 있고, 중앙 홀(103)을 중심으로 외측 반경방향으로 전처리 컨테이너(11a), 스핀 컬럼(31a), 집적 용기(51)가 순차적으로 안착 배치되는 컨테이너 안착부(105), 컬럼 안착부(107), 용기 안착부(111)가 형성되어 있다. 그리고, 복수의 컨테이너 안착부(105), 복수의 컬럼 안착부(107), 복수의 용기 안착부(111)는 중앙 홀(103)의 둘레를 따라 등간격으로 마련되어 있다.As shown in these figures, the rotor 101 of the centrifuge has a disk shape, and a central hole 103 that can be coupled to a rotating shaft (not shown) of the motor is formed in the center, and the central hole 103 is formed. The container seating portion 105, the column seating portion 107, and the container seating portion 111 in which the pretreatment container 11a, the spin column 31a, and the integrated container 51 are sequentially seated and disposed in the outer radial direction. Is formed. The plurality of container seating portions 105, the plurality of column seating portions 107, and the plurality of container seating portions 111 are provided at equal intervals along the circumference of the central hole 103.
그리고, 전처리 컨테이너(11a)의 주입부(23)는 중앙 홀(103)에 인접하게 위치하도록 전처리 컨테이너(11a)는 컨테이너 안착부(105)에 안착된다. 또한, 전처리 컨테이너(11a)와 연결되는 스핀 컬럼(31a)이 수평을 유지하도록 스핀 컬럼(31a)이 지지되는 컬럼 지지대(109)가 로터(101)의 컬럼 안착부(107)에 마련되어 있다. 그리고, 집적 용기(51)가 스핀 컬럼(31a)과 배출부(43)의 축선에 대해 경사지게 마련되도록 용기 지지대(113)가 로터(101)의 용기 안착부(111)에 마련되어 있다.Then, the pretreatment container 11a is seated on the container seating portion 105 so that the injection portion 23 of the pretreatment container 11a is positioned adjacent to the central hole 103. In addition, a column support 109 on which the spin column 31a is supported is provided on the column seat 107 of the rotor 101 so that the spin column 31a connected to the pretreatment container 11a is horizontal. And the container support 113 is provided in the container mounting part 111 of the rotor 101 so that the integration container 51 may be inclined with respect to the axis of the spin column 31a and the discharge part 43. As shown in FIG.
이러한 구성에 의하여, 일 예로 도 8 내지 도 10에 도시된 바와 같이, 한 쌍의 전처리 컨테이너(11a), 한 쌍의 스핀 컬럼(31a), 한 쌍의 집적 용기(51)가 대칭을 이루며 원심분리기의 로터(101)에 장착된 상태에서 원심분리하는 방법에 대해 설명한다.With this configuration, as shown in FIGS. 8 to 10, for example, a pair of pretreatment containers 11a, a pair of spin columns 31a, and a pair of integration vessels 51 are symmetrical and centrifuge The method of centrifuging in the state mounted on the rotor 101 is demonstrated.
먼저, 전처리 컨테이너(11a)와 스핀 컬럼(31a)과 집적 용기(51)를 로터(101)의 컨테이너 안착부(105), 컬럼 안착부(107), 용기 안착부(111)에 안착시킨다. 이 때, 전처리 컨테이너(11a)의 유출부(25)에 스핀 컬럼(31a)을 끼움 결합하여, 전처리 컨테이너(11a)와 스핀 컬럼(31a)을 연결한다.First, the pretreatment container 11a, the spin column 31a, and the integrated container 51 are seated on the container seating portion 105, the column seating portion 107, and the container seating portion 111 of the rotor 101. At this time, the spin column 31a is fitted to the outlet portion 25 of the pretreatment container 11a to connect the pretreatment container 11a and the spin column 31a.
다음, 원심분리하기 위한 핵산 등의 생물학적 또는 화학적 물질을 갖는 시료와 버퍼를 각각 전처리 컨테이너(11a)의 주입부(23)를 통해 챔버(21)에 주입한다. 이 때, 전처리 컨테이너(11a)의 주입부(23)가 로터(101)의 상방을 향하고 있어, 피펫(pipett)을 사용하여 시료 및 버퍼를 전처리 컨테이너(11a)에 간편하게 주입할 수 있게 된다. 한편, 챔버(21)에 시료 및 버퍼를 주입할 때, 챔버(21)에 주입되는 시료와 버퍼로 이루어진 혼합용액(3)이 유출부(25)를 통해 유출되지 않도록 한다.Next, a sample and a buffer having a biological or chemical substance such as nucleic acid for centrifugation are injected into the chamber 21 through the injection section 23 of the pretreatment container 11a, respectively. At this time, the injection portion 23 of the pretreatment container 11a faces upward of the rotor 101, so that the sample and the buffer can be easily injected into the pretreatment container 11a using a pipette. Meanwhile, when the sample and the buffer are injected into the chamber 21, the mixed solution 3 including the sample and the buffer injected into the chamber 21 does not flow out through the outlet 25.
이어서, 원심분리기의 로터(101)를 일방향으로 회전시킨다.Subsequently, the rotor 101 of the centrifuge is rotated in one direction.
이 때, 도 10에 도시된 바와 같이 전처리 컨테이너(11a)에 수용된 혼합용액(3)은 원심력에 의해 유출부(25)를 통해 유출되어, 스핀 컬럼(31a)의 유입부(35)를 통해 스핀 컬럼(31a)의 혼합용액 수용부(33)에 수용된다.At this time, as shown in FIG. 10, the mixed solution 3 contained in the pretreatment container 11a flows out through the outlet 25 by centrifugal force, and spins through the inlet 35 of the spin column 31a. It is accommodated in the mixed solution container 33 of the column 31a.
그리고, 스핀 컬럼(31a)의 혼합용액 수용부(33)에 수용된 혼합용액(3)은 원심력에 의해 필터부(45)를 통과하여 여과되면서 원심분리된 후, 원심분리된 원심분리액(5)은 스핀 컬럼(31a)의 배출부(43)를 통해 집적 용기(51)를 향해 배출된다.The mixed solution 3 contained in the mixed solution containing part 33 of the spin column 31a is centrifuged while being filtered through the filter part 45 by centrifugal force, followed by centrifuged centrifugal solution 5. Is discharged toward the integrated container 51 through the discharge portion 43 of the spin column 31a.
집적 용기(51)의 원심분리액 수용부(53)에 원심분리액(5)의 채집이 완료되면, 원심분리기의 로터(101)의 회전을 정지한 후, 집적 용기(51)의 원심분리액 수용부(53)에 수용된 원하는 원심분리액(5)을 얻을 수 있게 된다.When the collection of the centrifugal liquid 5 is completed in the centrifugal liquid container 53 of the integrated container 51, the rotation of the rotor 101 of the centrifuge is stopped, and then the centrifugal liquid of the integrated container 51 is stopped. It is possible to obtain the desired centrifugal liquid 5 contained in the accommodating portion 53.
한편, 도 4 및 도 5에는, 전술한 제1실시예와는 달리, 전처리 컨테이너(11b)의 유출부(25)의 외주에 수나사(27)가 형성되고, 스핀 컬럼(31b)의 유입부(35)의 내주에 암나사(37)가 형성되어, 전처리 컨테이너(11b)와 스핀 컬럼(31b)이 나사 결합되는 본 발명의 제2실시예에 따른 원심분리 키트(10b)가 도시되어 있다.On the other hand, in Figs. 4 and 5, unlike the first embodiment described above, the male screw 27 is formed on the outer circumference of the outlet portion 25 of the pretreatment container 11b, the inlet portion of the spin column 31b ( A centrifugal separation kit 10b according to the second embodiment of the present invention is shown in which an internal thread 37 is formed at an inner circumference of 35 so that the pretreatment container 11b and the spin column 31b are screwed together.
이와 같이, 본 발명의 제1 및 제2실시예에 따르면, 원심분리하기 위한 생물학적 또는 화학적 물질을 갖는 시료와 버퍼를 스핀 컬럼(31a,31b)에 직접 주입하지 않고, 스핀 컬럼(31a,31b)의 전단에 마련된 전처리 컨테이너(11a,11b)를 통해 주입함으로써, 시료와 버퍼의 주입이 용이하고, 작업성을 향상시키며, 자동화를 용이하게 할 수 있게 된다.As described above, according to the first and second embodiments of the present invention, the spin columns 31a and 31b are not directly injected into the spin columns 31a and 31b without the sample and the buffer having the biological or chemical substance for centrifugation. By injecting through the pretreatment containers 11a and 11b provided at the front end of the sample, the sample and the buffer can be easily injected, workability can be improved, and automation can be facilitated.
한편, 전술한 실시예들에서는 전처리 컨테이너(11a,11b)의 유출부(25)와 스핀 컬럼(31a,31b)의 유입부(35)가 나사 결합 또는 끼움 결합되어 전처리 컨테이너(11a,11b)와 스핀 컬럼(31a,31b)이 연결되는 것으로 설명하고 있지만, 도시되어 있지 않지만 전처리 컨테이너의 유출부는 스핀 컬럼의 유입부에 느슨하게 삽입되어, 전처리 컨테이너 내의 혼합용액은 스핀 컬럼으로 유입될 수 있다.Meanwhile, in the above-described embodiments, the outlet portion 25 of the pretreatment containers 11a and 11b and the inlet portion 35 of the spin columns 31a and 31b are screwed or fitted to the pretreatment containers 11a and 11b. Although the spin columns 31a and 31b are described as being connected, although not shown, the outlet of the pretreatment container is loosely inserted into the inlet of the spin column, so that the mixed solution in the pretreatment container may flow into the spin column.
한편, 도 11 및 도 12에는 본 발명의 제3실시예에 따른 원심분리 키트가 도시되어 있다. 이들 도면에 도시된 바와 같이, 본 발명의 제3실시예에 따른 원심분리 키트(10c)는 전술한 실시예들과는 달리, 전처리 컨테이너(11c)와 집적 용기(51) 사이에 스핀 컬럼이 개재되어 있지 않다.Meanwhile, FIGS. 11 and 12 illustrate a centrifugal separation kit according to a third embodiment of the present invention. As shown in these figures, the centrifugation kit 10c according to the third embodiment of the present invention, unlike the above-described embodiments, does not have a spin column interposed between the pretreatment container 11c and the integration vessel 51. not.
즉, 본 발명의 제3실시예에 따른 원심분리 키트(10c)는 전처리 컨테이너(11c)와, 필터부(45)와, 집적 용기(51)를 포함한다.That is, the centrifugal separation kit 10c according to the third embodiment of the present invention includes a pretreatment container 11c, a filter part 45, and an integration container 51.
본 발명의 제3실시예에 따른 원심분리 키트(10c)의 전처리 컨테이너(11c)는 전술한 제1실시예에 따른 전처리 컨테이너(11a)와 동일 형상과 구조를 가지며, 이에 이하에서는 그 구체적인 구조에 대한 설명을 생략한다. 한편, 본 발명의 제3실시예에 따른 원심분리 키트(10c)의 전처리 컨테이너(11c)는 챔버(21) 내의 혼합용액(3)을 원심분리시 발생하는 원심력에 의해 원심분리하는 역할을 한다.The pretreatment container 11c of the centrifugal separation kit 10c according to the third embodiment of the present invention has the same shape and structure as the pretreatment container 11a according to the first embodiment described above. Omit the description. Meanwhile, the pretreatment container 11c of the centrifuge kit 10c according to the third embodiment of the present invention serves to centrifuge the mixed solution 3 in the chamber 21 by centrifugal force generated during centrifugation.
필터부(45)는 전처리 컨테이너(11c)의 유출부(25)에 마련되어, 원심분리되는 혼합용액을 여과한다.The filter part 45 is provided in the outflow part 25 of the pretreatment container 11c, and filters the mixed solution centrifuged.
집적 용기(51)는 일측이 개구된 속이 빈 원형의 튜브 형상을 가지며, 집적 용기(51)의 내측에는 전처리 컨테이너(11a)로부터 원심분리되어 필터부(45)를 거쳐 배출된 원심분리액(5)을 수용하는 원심분리액 수용부(53)가 형성되어 있다. 집적 용기(51)는 전처리 컨테이너(11a)의 유출부(25)에 인접하게 위치하며, 유출부(25)의 축선에 대해 경사지게 마련된다. 집적 용기(51)는 전술한 제1실시예에 따른 집적 용기와 동일 형상과 구조를 가지며, 이에 이하에서는 그 구조에 대한 설명을 생략한다.The integrated container 51 has a hollow circular tube shape with one side opened, and the centrifugal liquid 5 centrifuged from the pretreatment container 11a inside the integrated container 51 and discharged through the filter part 45. The centrifugal liquid accommodating part 53 which accommodates () is formed. The accumulation container 51 is located adjacent to the outlet 25 of the pretreatment container 11a and is provided to be inclined with respect to the axis of the outlet 25. The integrated container 51 has the same shape and structure as the integrated container according to the first embodiment described above, and a description thereof will be omitted below.
도 13에는 본 발명의 제3실시예에 따른 원심분리 키트(10c)가 원심분리기의 로터(101b)에 장착된 모습을 도시한 종단면도가 도시되어 있다. 원심분리기의 로터(101b)는 원반 형상을 가지며, 중앙에 모터의 회전축(미도시)과 결합될 수 있는 중앙 홀(103)이 형성되어 있고, 중앙 홀(103)을 중심으로 외측 반경방향으로 전처리 컨테이너(11c), 집적 용기(51)가 순차적으로 안착 배치되는 컨테이너 안착부(105), 용기 안착부(111)가 형성되어 있다. 그리고, 복수의 컨테이너 안착부(105), 복수의 용기 안착부(111)는 중앙 홀(103)의 둘레를 따라 등간격으로 마련되어 있다. FIG. 13 is a longitudinal sectional view showing the centrifuge kit 10c according to the third embodiment of the present invention mounted on the rotor 101b of the centrifuge. The rotor 101b of the centrifuge has a disk shape, and has a central hole 103 formed at the center thereof, which can be combined with a rotational axis (not shown) of the motor, and pretreatment in the outer radial direction about the central hole 103. The container seating portion 105 and the container seating portion 111 in which the container 11c, the integrated container 51 are sequentially seated and disposed are formed. The plurality of container seating portions 105 and the plurality of container seating portions 111 are provided at equal intervals along the circumference of the central hole 103.
그리고, 전처리 컨테이너(11c)의 주입부(23)는 중앙 홀(103)에 인접하게 위치하도록 전처리 컨테이너(11c)는 컨테이너 안착부(105)에 안착된다. 또한, 집적 용기(51)가 전처리 컨테이너(11c)의 유출부(25)의 축선에 대해 경사지게 마련되도록 용기 지지대(113)가 로터(101b)의 용기 안착부(111)에 마련되어 있다.The pretreatment container 11c is seated on the container seating portion 105 such that the injection portion 23 of the pretreatment container 11c is positioned adjacent to the central hole 103. Moreover, the container support 113 is provided in the container mounting part 111 of the rotor 101b so that the integration container 51 may be inclined with respect to the axis line of the outflow part 25 of the pretreatment container 11c.
이에, 전처리 컨테이너(11c)와 집적 용기(51)를 로터(101b)의 컨테이너 안착부(105)와 용기 안착부(111)에 각각 안착시킨 후, 원심분리하기 위한 핵산 등의 생물학적 또는 화학적 물질을 갖는 시료와 버퍼를 각각 전처리 컨테이너(11c)의 주입부(23)를 통해 챔버(21)에 주입한 후, 원심분리기의 로터(101b)를 일방향으로 회전시키면, 전처리 컨테이너(11c)에 수용된 혼합용액(3)은 원심력에 의해 필터부(45)를 통과하여 여과되면서 원심분리된 후, 원심분리된 원심분리액(5)은 전처리 컨테이너(11c)의 유출부(25)를 통해 집적 용기(51)를 향해 배출된다.Thus, the pretreatment container 11c and the integrated container 51 are seated on the container seating portion 105 and the container seating portion 111 of the rotor 101b, respectively, and then biological or chemical substances such as nucleic acids for centrifugation are removed. After each sample and buffer have been injected into the chamber 21 through the injection part 23 of the pretreatment container 11c, the rotor 101b of the centrifuge is rotated in one direction, and the mixed solution contained in the pretreatment container 11c is carried out. (3) is centrifuged while being filtered through the filter unit 45 by centrifugal force, and then the centrifuged centrifugal liquid 5 passes through the outlet 25 of the pretreatment container 11c. Is discharged towards.
여러 버퍼들의 워싱(Washing) 단계를 거쳐 집적 용기(51)의 원심분리액 수용부(53)에 원심분리액(5)의 채집이 완료되면, 원심분리기의 로터(101b)의 회전을 정지한 후, 집적 용기(51)의 원심분리액 수용부(53)에 수용된 원하는 원심분리액(5)을 얻을 수 있게 된다.After the collection of the centrifugation liquid 5 to the centrifugation liquid receiving portion 53 of the integrated container 51 through the washing steps of the various buffers, the rotation of the rotor 101b of the centrifuge is stopped. The desired centrifugal liquid 5 contained in the centrifugal liquid container 53 of the integrated container 51 can be obtained.
이와 같이, 원심분리하기 위한 생물학적 또는 화학적 물질을 갖는 시료와 버퍼를 전처리 컨테이너(11c)의 상판부에 형성된 주입부(15)를 통해 주입하고, 전처리 컨테이너(11c)의 후단에 집적 용기(51)를 구비함으로써, 시료와 버퍼의 주입이 용이하고, 작업성을 향상시키며, 자동화를 용이하게 할 수 있을 뿐만 아니라 전술한 실시예들에 비해 원심분리 키트(10c)의 전체 길이를 줄일 수 있게 된다.As such, the sample and the buffer having the biological or chemical substance for centrifugation are injected through the injection unit 15 formed in the upper plate of the pretreatment container 11c, and the integrated container 51 is disposed at the rear end of the pretreatment container 11c. By providing, the sample and the buffer is easy to inject, improve the workability, facilitate the automation as well as to reduce the overall length of the centrifugation kit (10c) compared to the above-described embodiments.
한편, 도 14에는 본 발명의 제4실시예에 따른 원심분리 키트로서 전처리 컨테이너(11d)가 원형의 단면형상을 갖는 원심분리 키트(10d)가 도시되어 있다.Meanwhile, FIG. 14 illustrates a centrifugal separation kit 10d having a circular cross-sectional shape of a pretreatment container 11d as a centrifugal separation kit according to a fourth embodiment of the present invention.
본 발명의 제4실시예에 따른 원심분리 키트(10d)는 전술한 제3실시예의 전처리 컨테이너(11c)와 형상만 차이가 있을 뿐 기타 구성은 동일하므로, 이하에서는 그 설명을 생략한다.Since the centrifugal separation kit 10d according to the fourth embodiment of the present invention differs only in shape from the pretreatment container 11c of the third embodiment, other configurations are the same, the description thereof will be omitted below.
한편, 전처리 컨테이너는 전술한 사각형, 원형의 단면형상 이외에 타원형의 단면형상을 가질 수도 있다.Meanwhile, the pretreatment container may have an elliptical cross-sectional shape in addition to the rectangular and circular cross-sectional shapes described above.
도 15 내지 도 18에는 본 발명의 제5실시예에 따른 원심분리 키트가 도시되어 있다. 본 발명의 제5실시예에 따른 원심분리 키트(10e)의 전처리 컨테이너(11e)는 전술한 제1실시예와는 달리, 챔버(21)에 수용된 혼합용액(3)이 주입부(23)로 유출되는 것을 방지하는 방지턱(29)을 더 포함한다.15 to 18 show a centrifugal separation kit according to a fifth embodiment of the present invention. In the pretreatment container 11e of the centrifugal separation kit 10e according to the fifth embodiment of the present invention, the mixed solution 3 contained in the chamber 21 is injected into the injection unit 23 unlike the first embodiment described above. It further comprises a stopper 29 for preventing the leakage.
본 실시예에서의 방지턱(29)은 일 측벽부(13c)에 예컨대, 유출부(25)가 형성된 측벽부(13a)의 반대편에 위치한 측벽부(13c)에 ′V′자 단면형상으로 함몰 형성되어 있다. 또한, 방지턱(29)은, 챔버(21) 내의 혼합용액(3)이 원심분리시 발생하는 원심력에 의해 유출부(25)를 향해 원활하게 유출되도록, 주입부(23)와 유출부(25) 사이에 위치한다.In the present embodiment, the barrier rib 29 is recessed in a cross-sectional shape of a 'V' shape on the side wall portion 13c, for example, on the side wall portion 13c positioned opposite to the side wall portion 13a on which the outlet portion 25 is formed. It is. In addition, the jaw 29 has the inlet 23 and the outlet 25 so that the mixed solution 3 in the chamber 21 flows smoothly toward the outlet 25 by the centrifugal force generated during centrifugation. Located in between.
본 실시예에서는 방지턱(29)이 일 측벽부(13c)에 함몰 형성되는 것으로 설명하고 있지만 이에 한정되지 않으며, 방지턱(29)은 전처리 컨테이너(11e)의 내측에 ′V′자 단면형상으로 돌출 형성되거나, 일정 길이와 폭을 갖는 띠 형상으로 돌출 형성될 수도 있다.Although the jaw 29 is described as being recessed in one side wall portion 13c in the present embodiment, the present invention is not limited thereto, and the jaw 29 protrudes in a cross-sectional shape of a 'V' shape inside the pretreatment container 11e. Or it may be formed to protrude in a band shape having a predetermined length and width.
또한, 본 발명의 제5실시예에 따른 원심분리 키트(10e)의 전처리 컨테이너(11e)는 원심분리시 원심분리기의 로터(101a, 도 7참조)의 하측에 위치한 고정턱(115)과의 상호 작용에 의해 전처리 컨테이너(11e)가 요동하도록 전처리 컨테이너(11e)에 돌출 형성된 돌출 리브(27)를 더 포함한다.In addition, the pretreatment container (11e) of the centrifugal separation kit (10e) according to the fifth embodiment of the present invention is mutually with the fixing jaw 115 located below the rotor 101a (see Fig. 7) of the centrifuge during centrifugation. It further includes a protruding rib 27 protruding from the pretreatment container 11e so as to swing the pretreatment container 11e by the action.
본 실시예에서의 돌출 리브(27)는 주입부(23)와 대각 방향에 위치하는 전처리 컨테이너(11e)의 일 측벽부(13a)로부터 돌출 형성되어 있지만 이에 한정되지 않으며, 돌출 리브(27)는 하판부(17)로부터 돌출 형성될 수 있다.The protruding ribs 27 in the present embodiment protrude from one side wall portion 13a of the pretreatment container 11e positioned in the direction opposite to the injection portion 23, but the present invention is not limited thereto. It may be formed to protrude from the lower plate portion 17.
이에 의해, 전처리 컨테이너(11e)를 원심분리기의 로터(101a)에 장착하여 원심분리시 전처리 컨테이너(11e)가 회전함에 따라, 돌출 리브(27)는 전처리 컨테이너(11e)의 회전 반경 내에 로터(101a)의 하측에 전처리 컨테이너(11e)를 향해 간격을 두고 돌출되게 위치한 원심분리기의 복수의 고정턱(115)을 넘나들며, 이에 전처리 컨테이너(11e)가 요동하게 되어 전처리 컨테이너(11e)의 챔버(21)에 수용된 혼합용액(3)은 원활하게 교반된다.As a result, the pretreatment container 11e is mounted on the rotor 101a of the centrifuge and the pretreatment container 11e rotates during centrifugation, so that the protruding ribs 27 rotate within the rotation radius of the pretreatment container 11e. It passes over a plurality of fixing jaw 115 of the centrifuge located protruded at intervals toward the pretreatment container (11e) at the lower side of the), the pretreatment container (11e) is rocked, the chamber 21 of the pretreatment container (11e) The mixed solution 3 accommodated in is stirred smoothly.
이와 같이, 본 발명의 제5실시예에 따른 원심분리 키트(10e)의 전처리 컨테이너(11e)는 전술한 제1실시예와는 달리 돌출 리브(27)와 방지턱(29)을 더 포함함으로써, 원심분리시 전처리 컨테이너(11e)가 회전하면서 돌출 리브(27)가 고정턱(27)을 넘나들며 전처리 컨테이너(11e)가 요동함에 따라, 전처리 컨테이너(11e)의 챔버(21) 내에 수용된 혼합용액(3)이 원활하게 교반된다. 또한, 전처리 컨테이너(11e)의 요동시 챔버(21) 내의 혼합용액(3)은 방지턱(29)에 의해 주입부(23)로 유출되지 않고 유출부(25)로 원활하게 유출된다.Thus, unlike the first embodiment described above, the pretreatment container 11e of the centrifugal separation kit 10e according to the fifth embodiment of the present invention further includes a protruding rib 27 and a jaw 29 so that the centrifuge kit As the pretreatment container 11e rotates during separation, the protruding rib 27 passes over the fixing jaw 27 and the pretreatment container 11e swings, so that the mixed solution 3 contained in the chamber 21 of the pretreatment container 11e. This is stirred smoothly. In addition, when the pretreatment container 11e swings, the mixed solution 3 in the chamber 21 does not flow out into the inlet 23 by the jaw 29, but smoothly flows out of the outlet 25.
본 발명은 기재된 실시예에 한정되는 것이 아니고, 본 발명의 사상 및 범위를 벗어나지 않고 다양하게 수정 및 변형할 수 있음은 이 기술의 분야에서 통상의 지식을 가진 자에게 자명하다. 따라서 그러한 수정 예 또는 변형 예들은 본 발명의 특허청구범위에 속한다 하여야 할 것이다.It is apparent to those skilled in the art that the present invention is not limited to the described embodiments, and that various modifications and changes can be made without departing from the spirit and scope of the present invention. Therefore, such modifications or variations will have to be belong to the claims of the present invention.

Claims (12)

  1. 원심분리하기 위한 생물학적 또는 화학적 물질을 갖는 시료와 버퍼가 함유된 혼합용액을 수용하는 챔버와, 상기 챔버로 상기 시료와 버퍼가 주입되는 주입부와, 원심분리시 발생하는 원심력에 의해 상기 챔버 내의 상기 혼합용액이 유출되는 유출부를 갖는 전처리 컨테이너와;A chamber containing a mixed solution containing a sample and a buffer having a biological or chemical substance for centrifugation, an injection portion into which the sample and the buffer are injected into the chamber, and the centrifugal force generated during centrifugation A pretreatment container having an outlet portion through which the mixed solution flows out;
    상기 유출부를 통해 유출되는 상기 혼합용액이 유입되는 유입부와, 상기 유입부로부터 유입된 상기 혼합용액을 수용하는 혼합용액 수용부와, 상기 혼합용액으로부터 원심분리된 원심분리액이 배출되는 배출부와, 상기 유입부와 상기 배출부 사이에 마련되어 원심분리되는 상기 혼합용액을 여과하는 필터부를 갖는 스핀 컬럼을 포함하는 것을 특징으로 하는 원심분리 키트.An inlet part through which the mixed solution flowing out through the outlet part flows in, a mixed solution accommodating part accommodating the mixed solution introduced from the inlet part, and a discharge part discharging the centrifuged solution centrifuged from the mixed solution; And a spin column provided between the inlet and the outlet to filter the mixed solution to be centrifuged.
  2. 제1항에 있어서,The method of claim 1,
    상기 스핀 컬럼으로부터 원심분리되어 상기 필터부를 거쳐 배출된 원심분리액을 수용하는 원심분리액 수용부가 형성된 집적 용기를 더 포함하는 것을 특징으로 하는 원심분리 키트.Centrifugal separation kit further comprises an integrated container formed with a centrifugation solution receiving portion for receiving the centrifugation liquid discharged through the filter portion by centrifugation from the spin column.
  3. 제1항에 있어서,The method of claim 1,
    상기 전처리 컨테이너의 유출부는 상기 스핀 컬럼의 유입부에 나사 결합, 또는 끼움 결합, 또는 느슨하게 삽입되는 것을 특징으로 하는 원심분리 키트.Centrifugation kit, characterized in that the outlet of the pretreatment container is screwed, or fitted, or loosely inserted into the inlet of the spin column.
  4. 제2항에 있어서,The method of claim 2,
    상기 집적 용기는 상기 배출부에 인접하게 위치하며, 상기 배출부의 축선에 대해 경사지게 마련되는 것을 특징으로 하는 원심분리 키트.The integrated container is located adjacent to the discharge portion, the centrifugal kit, characterized in that provided inclined with respect to the axis of the discharge portion.
  5. 원심분리하기 위한 생물학적 또는 화학적 물질을 갖는 시료와 버퍼가 함유된 혼합용액을 수용하는 챔버와, 상기 챔버로 상기 시료와 버퍼가 주입되는 주입부와, 상기 주입부와 45°～135°의 각도를 이루면서 위치하여 원심분리시 발생하는 원심력에 의해 원심분리되는 상기 챔버 내의 상기 혼합용액이 유출되는 유출부를 갖는 전처리 컨테이너와;A chamber containing a sample having a biological or chemical substance for centrifugation and a mixed solution containing a buffer, an injection portion into which the sample and buffer are injected into the chamber, and an angle of 45 ° to 135 ° with the injection portion. A pretreatment container positioned to have an outlet portion through which the mixed solution in the chamber is centrifuged by centrifugal force generated during centrifugation;
    상기 전처리 컨테이너의 유출부에 마련되어, 원심분리되는 상기 혼합용액을 여과하는 필터부와;A filter unit provided at an outlet of the pretreatment container and filtering the mixed solution to be centrifuged;
    상기 필터부를 거쳐 배출된 원심분리액을 수용하는 원심분리액 수용부가 형성된 집적 용기를 포함하는 것을 특징으로 하는 원심분리 키트.Centrifugal separation kit comprising an integrated container formed with a centrifugation solution receiving portion for receiving the centrifugation liquid discharged through the filter.
  6. 제5항에 있어서,The method of claim 5,
    상기 집적 용기는 상기 유출부에 인접하게 위치하며, 상기 유출부의 축선에 대해 경사지게 마련되는 것을 특징으로 하는 원심분리 키트.And the accumulator is positioned adjacent to the outlet and is inclined with respect to the axis of the outlet.
  7. 제5항에 있어서,The method of claim 5,
    상기 전처리 컨테이너는 통형상의 네 측벽부와, 상기 네 측벽부에 의해 형성된 양단 개구를 차단하는 상판부 및 하판부를 가지며, 상기 주입부는 원심분리시 발생하는 원심력에 의해 상기 챔버 내의 상기 혼합용액이 유출되지 않도록 상기 상판부에 마련되고, 상기 유출부는 원심분리시 발생하는 원심력에 의해 상기 챔버 내의 상기 혼합용액이 원활하게 유출되도록 상기 네 측벽부 중 어느 하나의 측벽부의 상측에 마련되는 것을 특징으로 하는 원심분리 키트.The pretreatment container has four cylindrical side wall portions, an upper plate portion and a lower plate portion for blocking openings at both ends formed by the four side wall portions, and the injection portion does not leak the mixed solution in the chamber by centrifugal force generated during centrifugation. Is provided in the upper plate portion, the outlet portion is a centrifugal separation kit is provided on the side of any one side wall portion of the four side wall portion so that the mixed solution in the chamber smoothly flows out by the centrifugal force generated during centrifugation .
  8. 제1항 있어서,The method of claim 1,
    상기 주입부와 상기 유출부는 45°∼135°의 각도를 이루는 것을 특징으로 하는 원심분리 키트.Centrifugal separation kit, characterized in that the inlet and the outlet makes an angle of 45 ° to 135 °.
  9. 제5항에 있어서,The method of claim 5,
    상기 주입부로부터 상기 유출부를 향할수록 상기 전처리 컨테이너의 폭은 증대하는 것을 특징으로 하는 원심분리 키트.Centrifugal separation kit, characterized in that the width of the pretreatment container increases from the inlet to the outlet.
  10. 제5항에 있어서,The method of claim 5,
    상기 필터부는 분리막(membrane) 또는 여과제(media filter)로 이루어지는 것을 특징으로 하는 원심분리 키트.The filter unit centrifugal separation kit, characterized in that consisting of a membrane (membrane) or a filter (media filter).
  11. 제5항에 있어서,The method of claim 5,
    상기 전처리 컨테이너는 상기 챔버에 수용된 혼합용액이 상기 주입부로 유출되는 것을 방지하는 방지턱을 포함하는 것을 특징으로 하는 원심분리 키트.The pretreatment container is a centrifugation kit, characterized in that it comprises a jaw to prevent the mixed solution contained in the chamber to flow out of the injection portion.
  12. 제11항에 있어서,The method of claim 11,
    원심분리시 원심분리기의 로터의 하측에 위치한 고정턱과의 상호 작용에 의해 상기 전처리 컨테이너가 요동하도록 상기 전처리 컨테이너에 돌출 형성된 돌출 리브를 더 포함하는 것을 특징으로 하는 원심분리 키트.And a protruding rib protruding from the pretreatment container to swing the pretreatment container by interaction with a fixed jaw positioned at the lower side of the rotor of the centrifuge during centrifugation.
PCT/KR2014/005156 2014-02-25 2014-06-12 Centrifugation kit WO2015129965A1 (en)

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KR20180041384A (en) * 2016-10-14 2018-04-24 (주)어핀텍 Contrifugal method using sputum pre-processing container for isolation of tubercle bacillus

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4787971A (en) * 1987-01-23 1988-11-29 Alan Donald Miniaturized column chromatography separation apparatus and method of assaying biomolecules employing the same
JPH0628749B2 (en) * 1989-10-06 1994-04-20 ベックマン インスツルメンツ インコーポレーテッド Centrifuge rotor, manufacturing method thereof and density gradient centrifugation method
US6103195A (en) * 1997-08-08 2000-08-15 Shukla; Ashok K. Micro-volume spin columns for sample preparation
KR101197974B1 (en) * 2011-11-01 2012-11-05 박현정 A container for centrifugal separator capable of rapid centrifugal separation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4787971A (en) * 1987-01-23 1988-11-29 Alan Donald Miniaturized column chromatography separation apparatus and method of assaying biomolecules employing the same
JPH0628749B2 (en) * 1989-10-06 1994-04-20 ベックマン インスツルメンツ インコーポレーテッド Centrifuge rotor, manufacturing method thereof and density gradient centrifugation method
US6103195A (en) * 1997-08-08 2000-08-15 Shukla; Ashok K. Micro-volume spin columns for sample preparation
KR101197974B1 (en) * 2011-11-01 2012-11-05 박현정 A container for centrifugal separator capable of rapid centrifugal separation

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