WO2015126340A1 - Équipement de laboratoire utilisé pour isoler manuellement des acides nucléiques par l'intermédiaire de particules magnétiques et pour les préparer à des analyses de génétique moléculaire - Google Patents

Équipement de laboratoire utilisé pour isoler manuellement des acides nucléiques par l'intermédiaire de particules magnétiques et pour les préparer à des analyses de génétique moléculaire Download PDF

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Publication number
WO2015126340A1
WO2015126340A1 PCT/TR2015/000020 TR2015000020W WO2015126340A1 WO 2015126340 A1 WO2015126340 A1 WO 2015126340A1 TR 2015000020 W TR2015000020 W TR 2015000020W WO 2015126340 A1 WO2015126340 A1 WO 2015126340A1
Authority
WO
WIPO (PCT)
Prior art keywords
magnetic
tubes
magnetic particles
nucleic acids
magnets
Prior art date
Application number
PCT/TR2015/000020
Other languages
English (en)
Inventor
Alper AKYÜZ
Original Assignee
Anatoli̇a Tani Ve Bi̇yoteknoloji̇ Ürünleri̇ Araştirma Geli̇şti̇rme Sanayi̇ Ve Ti̇caret Anoni̇m Şi̇rketi̇
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anatoli̇a Tani Ve Bi̇yoteknoloji̇ Ürünleri̇ Araştirma Geli̇şti̇rme Sanayi̇ Ve Ti̇caret Anoni̇m Şi̇rketi̇ filed Critical Anatoli̇a Tani Ve Bi̇yoteknoloji̇ Ürünleri̇ Araştirma Geli̇şti̇rme Sanayi̇ Ve Ti̇caret Anoni̇m Şi̇rketi̇
Publication of WO2015126340A1 publication Critical patent/WO2015126340A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces

Definitions

  • the invention is related to one laboratory equipment that is utilized to isolate nucleic acids manually via magnetic particles and to prepare them for molecular genetic analyses.
  • the invention especially is relevant to one laboratory equipment; that contains a magnetic slide, a revolving frame, a unit base, a lower rack, an upper rack, a magnetic block, magnetic stoppers; to enable isolation of nucleic acids manually via magnetic particles to be ready for molecular genetic analyses, and to allow extraction of DNA and RNA from 24 biological samples simultaneously.
  • the magnetic stand models used in current technique are; far from being practical, prone to errors, with long operation durations, with the requirement of the user to change the positions of the tubes continuously, ineffective equipments with low capacity. Additionally, due to the inability to process high sample volumes, they do not enable to extract DNA/RNA in high concentrations. Therefore, using them especially for diagnostic purposes and for high sensitivity viral quantification processes, must be avoided. Nucleic acid extraction is the starting point for all genetic research and molecular tests. Many widespread molecular genetic tests and experiments, can only be performed after the DNA or RNA of target organism is purified. DNA/RNA extraction in general includes; cell membrane and wall degradation, inactivation of nuclease activity, removal of proteins, concentration of nucleic acids and proper storage of the isolated DNA/RNA.
  • Extraction is composed of four steps named as; lysis (exposing of DNA by breaking down of the ceils and membrane bound structures, denaturation and removal of the proteins from DNA/RNA), binding (binding of DNA/RNA on a surface), washing (removal of undesired contaminants in the medium such as salt, protein, etc.) and elution (recovery of DNA/RNA from the bound surface).
  • Magnetic extraction technology is based on the separation of magnetic particles and the DNA/RNA bound to them, via a magnet.
  • the separation procedure in which the magnetic particles are used as a solid support, is much faster, simpler and an effective method than the other methods, with the adaptability to automated systems, and enables DNA/RNA extraction in large scale, easily.
  • the sample After being treated with the lysis solutions and the proteinase enzyme, the sample forms a magnetic complex by binding to the magnetic particles which act as a suitable carrier for free DNA/RNA. The undesired bound contaminants are removed by the washing of the magnetic complex.
  • the DNA/RNA is eiuted by removal from the magnetic complex, and stored for further applications.
  • the present invention is related to the laboratory equipment that; meets the requirements mentioned above, eliminates all the disadvantages while bringing some additional advantages, is utilized to isolate nucleic acids manually via magnetic particles and to prepare them for molecular genetic analyses.
  • Primary purpose of the invention is to establish one laboratory equipment used to isolate nucleic acids manually via magnetic particles and to prepare them for molecular genetic analyses. Other purposes of the invention, is to provide an important technical advantage for the manual use, and also to present the laboratory equipment so practical that can compete with fully automated robots, with the capability of producing repeatable results and has a reasonable cost. Another purpose of the invention is to render the capacity of processing 24 samples in a very limited space.
  • Another purpose of the invention is the ability to provide magnetization and demagnetization by just one movement of the magnetic block, eliminating the necessity of changing the tubes' positions.
  • Another purpose of the invention is the ability to complete the extraction protocol very swiftly, faster than the current methods, as the tubes remain at their positions, thanks to the revolving frame.
  • a similar purpose of the invention is to enable the rotation of the sample tubes easily to the correct position, and processing of samples in 4 groups of 6, their successive magnetization and demagnetization, thanks to its magnetic stoppers. Moreover, another purpose is to minimize user errors by its alphetically marked (A,B,C,D) four edges.
  • Another purpose of the invention is to allow avoiding of the disturbance of magnetized and collected magnetic particles during pipetting and prevention against related errors, thanks to the holes with special angles (tube holes with axes designed to have 60-80 degrees angles with respect to the ground).
  • Another purpose of the invention is to control the rotating movement of the stand in angles of ninety degrees, and allowing to stop the back and forth motion of the magnets in a controlled manner, thanks to the magnetic stoppers. With this ease of use, user errors are avoided.
  • a similar purpose of the invention is, its convenience to use high starting volume material. This enables DNA/RNA recovery in high concentration and sensitivity, from high volume body fluids.
  • Another purpose of the invention is to position the centers of the holes where all tubes are placed with a distance of 18 mm, in groups of six. This condition allows practical processing of many samples in tubes that will be placed in the holes, simultaneously by using a multichannel pipette.
  • Another purpose of the invention is to enable using DNA/RNA extraction kits based on binding nucleic acids to silica coated magnetic particles. Similar purposes of the invention are, to reduce the workload of the laboratories, to avoid the errors that occur during the manual processing by the laboratory technicians, and the false positive and false negative test results that could result from mistakes in application.
  • the invention is the laboratory equipment which is utilized to isolate nucleic acids manually via magnetic particles and to prepare them for molecular genetic analyses, and is characterized to contain the following elements, in order to accomplish the purposes described above and to enable simultaneous DNA and RNA extraction from 24 biological samples; ⁇ The magnetic slide, that allows simultaneous magnetization of the magnetic particles in the tubes analyzed, at the neccessary steps, and their collection around the magnets; whereas enabling the termination of the magnetization to purify the liquid from magnetic particles in the neccessary steps,
  • the unit base which; via its embedded magnets, traps the magnets on the revolving frame by the magnetic attraction force, and allows the fixing of the revolving frame at angles of 90 degrees, and also enables the fixation of the magnetic slide at the correct positions; and functions as the linear bearing and support for the body and the other parts so that they can revolve around a fixed axis,
  • the lower rack which has holes suitable to carry the 1.5 ml tubes in order to allow DNA/RNA elution in low volume and to obtain nucleic acids in high concentration from biological samples,
  • the upper rack which has holes suitable to carry the 5 ml tubes in order to enable DNA/RNA extraction from high volume biological samples and to obtain nucleic acids in high concentration
  • the magnetic stoppers that are composed of magnets with opposite poles facing eachother, that are fixed in the magnetic slide, the unit base and the revolving frame; in order to allow biological samples to be revolved at angles of 90 degrees and to be processed in 4 groups of 6 successively and enables their synchronized magnetization or demagnetization.
  • Figure 1 Is the isometric view of the laboratory equipment which is the subject of the invention.
  • Figure 2 ls the view of the magnetic stoppers on the unit base of the mentioned laboratory equipment.
  • Figure 3 Is the view of the magnetic slide and the magnetic stoppers on this slide, that are included within the mentioned laboratory equipment.
  • Figure 4 Is the view of the magnetic stoppers on the revolving frame of the mentioned laboratory equipment.
  • Figure 5 Is the general view of the unit base that holds the jut.
  • Figure 6 Is the side view of the unit base that holds the jut. Scaling of the drawings is not neccessary and details that are not needed to comprehend the existing invention may have been neglected. Moreover, the elements which are at least highly identical or with at least highly identical functions are shown with the same number. Explanation for the Part References
  • the invention is the laboratory equipment (a magnetic stand) (1) that is utilized to isolate nucleic acids from biological samples manually via magnetic particles and to prepare them for molecular genetic analyses.
  • the magnetic stand (1) mentioned allows use of DNA/RNA extraction kits based on the method of binding nucleic acids to silica coated magnetic particles.
  • the laboratory equipment (1) that is the subject of the invention consists of; the magnetic slide (10), the unit base (11), the revolving frame (12), the lower rack (13), the upper rack (14), the magnetic block (15) and the magnetic stoppers (100, 110, 120). Magnetic slide (10);
  • This block which can easily be placed in and taken out of the equipment with only one movement, due to the neodymium magnets embedded at its sides, it enables magnetization and demagnetization of alt samples in a synchronized manner, allows collection of all the magnetic particles in the liquid in the tubes by the magnets thus allowing neccessary processing of the liquid.
  • Magnetic stopper 100, 110, 120
  • Magnetic stoppers (100, 110, 120) composed of magnets with opposite poles facing eachother, that are fixed in the magnetic slide (10), the unit base (11) and the revolving frame (12); allows the magnetic slide (10) and the revolving frame (12), to be fixed at the targeted positions with respect to the unit base (11).
  • These magnetic stoppers (100, 110, 120); allows samples to be revolved easily at angles of 90 degrees and to be processed in 4 groups of 6 successively and enables their synchronized magnetization or demagnetization. This state, provides ease of use and also avoids user errors .
  • Magnetic slide (10) is placed into the magnetic slide housing (111) in the station base (11).
  • the upper rack (14) is placed inside the lower rack (13) and then both of them are placed into the revolving frame (12).
  • These three pieces(12, 13, 14) are placed on the station base (11 ), by centering the jut (112) on the station base.
  • 5 ml tubes are placed into the upper rack (14) and 1.5 ml tubes are placedinto the lower rack (13).
  • Biologic samples, magnetic particles and necessary solutions are pipetted into 5 ml tubes and mixed with a micropipette. After incubation magnetic block (15) is placed on the station base (11). Required magnetization occurs after short period of time and magnetic particules are collected on one side of the tubes. Used solution is discarded via pipetting.
  • Magnetization is terminated by taking out the magnetic block (15). Needed solution for the next step is pipetted into the tubes on the upper rack (14). After processing of the 6 tube group on the row marked with "A" at the cornerof the magnetic stand (1) is completed.the revolving frame (12) is revolved 90 degrees with every piece attached to it, in order to process the next 6 tube group on the row marked with "B" at the corner.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un équipement de laboratoire qui contient une glissière magnétique (10), un cadre tournant (12), un socle unitaire (11), un râtelier inférieur (13), un râtelier supérieur (14), un bloc magnétique (15), des bouchons magnétiques (100, 110, 120) ; pour permettre l'isolement manuel d'acides nucléiques par l'intermédiaire de particules magnétiques, de façon à les préparer à des analyses de génétique moléculaire, et pour permettre l'extraction d'ADN et d'ARN simultanément à partir de 24 échantillons biologiques.
PCT/TR2015/000020 2014-02-18 2015-01-20 Équipement de laboratoire utilisé pour isoler manuellement des acides nucléiques par l'intermédiaire de particules magnétiques et pour les préparer à des analyses de génétique moléculaire WO2015126340A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2014/01897 2014-02-18
TR201401897 2014-02-18

Publications (1)

Publication Number Publication Date
WO2015126340A1 true WO2015126340A1 (fr) 2015-08-27

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/TR2015/000020 WO2015126340A1 (fr) 2014-02-18 2015-01-20 Équipement de laboratoire utilisé pour isoler manuellement des acides nucléiques par l'intermédiaire de particules magnétiques et pour les préparer à des analyses de génétique moléculaire

Country Status (1)

Country Link
WO (1) WO2015126340A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108220160A (zh) * 2016-12-21 2018-06-29 广州康昕瑞基因健康科技有限公司 磁力架
CN112742489A (zh) * 2019-10-30 2021-05-04 重庆好德译信息技术有限公司 检验科用防污染储存设备
WO2022103510A1 (fr) * 2020-11-10 2022-05-19 Dh Technologies Development Pte. Ltd. Analyseurs de plaques à puits utilisant des aimants amovibles

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0030086A1 (fr) * 1979-11-13 1981-06-10 TECHNICON INSTRUMENTS CORPORATION (a New York corporation) Ensemble de tubes à essais, kit pour sa fabrication et procédé d'essais immunologiques manuels
EP0644425A1 (fr) * 1993-09-17 1995-03-22 F. Hoffmann-La Roche Ag Analyseur, comprenant un dispositif pour la séparation de microparticules magnétiques
US6884357B2 (en) * 1995-02-21 2005-04-26 Iqbal Waheed Siddiqi Apparatus and method for processing magnetic particles
EP1621890A1 (fr) * 2004-07-26 2006-02-01 bioMerieux B.V. Dispositif et procédé de séparation, de mélange et de concentration des particules magnétiques avec liquides et leurs utilisations dans des méthodes de purification
US20080056957A1 (en) * 2006-09-01 2008-03-06 Chemglass, Inc. Segmented reaction blocks for supporting vials of different sizes for chemical synthesis on a hot plate stirrer

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0030086A1 (fr) * 1979-11-13 1981-06-10 TECHNICON INSTRUMENTS CORPORATION (a New York corporation) Ensemble de tubes à essais, kit pour sa fabrication et procédé d'essais immunologiques manuels
EP0644425A1 (fr) * 1993-09-17 1995-03-22 F. Hoffmann-La Roche Ag Analyseur, comprenant un dispositif pour la séparation de microparticules magnétiques
US6884357B2 (en) * 1995-02-21 2005-04-26 Iqbal Waheed Siddiqi Apparatus and method for processing magnetic particles
EP1621890A1 (fr) * 2004-07-26 2006-02-01 bioMerieux B.V. Dispositif et procédé de séparation, de mélange et de concentration des particules magnétiques avec liquides et leurs utilisations dans des méthodes de purification
US20080056957A1 (en) * 2006-09-01 2008-03-06 Chemglass, Inc. Segmented reaction blocks for supporting vials of different sizes for chemical synthesis on a hot plate stirrer

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108220160A (zh) * 2016-12-21 2018-06-29 广州康昕瑞基因健康科技有限公司 磁力架
CN112742489A (zh) * 2019-10-30 2021-05-04 重庆好德译信息技术有限公司 检验科用防污染储存设备
WO2022103510A1 (fr) * 2020-11-10 2022-05-19 Dh Technologies Development Pte. Ltd. Analyseurs de plaques à puits utilisant des aimants amovibles

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