WO2015120431A1 - Nouvelles variantes de terpènes cyclases et procédés d'utilisation de celles-ci - Google Patents

Nouvelles variantes de terpènes cyclases et procédés d'utilisation de celles-ci Download PDF

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Publication number
WO2015120431A1
WO2015120431A1 PCT/US2015/015153 US2015015153W WO2015120431A1 WO 2015120431 A1 WO2015120431 A1 WO 2015120431A1 US 2015015153 W US2015015153 W US 2015015153W WO 2015120431 A1 WO2015120431 A1 WO 2015120431A1
Authority
WO
WIPO (PCT)
Prior art keywords
streptomyces
variant
terpene cyclase
terpene
active site
Prior art date
Application number
PCT/US2015/015153
Other languages
English (en)
Inventor
David W. Christianson
Ruiqiong LI
Kevin LITWIN
David E. Cane
Wayne CHOU
Golda HARRIS
Julie Anne HIMMELBERGER
Original Assignee
The Trustees Of The University Of Pennsylvania
Brown University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Trustees Of The University Of Pennsylvania, Brown University filed Critical The Trustees Of The University Of Pennsylvania
Publication of WO2015120431A1 publication Critical patent/WO2015120431A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03022—Germacradienol synthase (4.2.3.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)

Abstract

La présente invention concerne des procédés pour produire des variantes de terpènes cyclases et pour modifier l'activité et/ou le profil de produit de terpènes cyclases de type sauvage et variantes. La présente invention concerne en outre des compositions comprenant les variantes de terpènes cyclases, des composés préparés au moyen des variantes de terpènes cyclases et des utilisations des variantes de terpènes cyclases.
PCT/US2015/015153 2014-02-10 2015-02-10 Nouvelles variantes de terpènes cyclases et procédés d'utilisation de celles-ci WO2015120431A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461937897P 2014-02-10 2014-02-10
US61/937,897 2014-02-10

Publications (1)

Publication Number Publication Date
WO2015120431A1 true WO2015120431A1 (fr) 2015-08-13

Family

ID=53778534

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/015153 WO2015120431A1 (fr) 2014-02-10 2015-02-10 Nouvelles variantes de terpènes cyclases et procédés d'utilisation de celles-ci

Country Status (1)

Country Link
WO (1) WO2015120431A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999037139A1 (fr) * 1998-01-22 1999-07-29 Washington State University Research Foundation Sesquiterene synthases tirees du sapin grandissime abies grandis et procedes d'utilisation
US20130298861A1 (en) * 2010-11-08 2013-11-14 The Regents Of The University Of California Isoprenoid based alternative diesel fuel

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999037139A1 (fr) * 1998-01-22 1999-07-29 Washington State University Research Foundation Sesquiterene synthases tirees du sapin grandissime abies grandis et procedes d'utilisation
US20130298861A1 (en) * 2010-11-08 2013-11-14 The Regents Of The University Of California Isoprenoid based alternative diesel fuel

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AARON, J.: "Structure, Function, And Applications Of MEtal-Requiring Enzymes: Carbonic Anhydrase And Epi-Isozizaene Synthase.", PUBLICALY ACCESSIBLE PENN DISSERTATIONS., 20 April 2015 (2015-04-20), Retrieved from the Internet <URL:http://repository.upenn.edu/edissertations/142> *
GARMS, S.: "A Single Amino Acid Determines The Site Of Deprotonation In The Active Center Of Sesquiterpene Synthase SbTPS1 And SbTPS2 From Sorghum Bicolor.", PHYTOCHEMISTRY, vol. 75, no. 6-13, March 2012 (2012-03-01) *

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