WO2015110250A3 - Method for creating an image of an object, and optical apparatus - Google Patents

Method for creating an image of an object, and optical apparatus Download PDF

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Publication number
WO2015110250A3
WO2015110250A3 PCT/EP2015/000034 EP2015000034W WO2015110250A3 WO 2015110250 A3 WO2015110250 A3 WO 2015110250A3 EP 2015000034 W EP2015000034 W EP 2015000034W WO 2015110250 A3 WO2015110250 A3 WO 2015110250A3
Authority
WO
WIPO (PCT)
Prior art keywords
point
optical
distance position
image
sample region
Prior art date
Application number
PCT/EP2015/000034
Other languages
German (de)
French (fr)
Other versions
WO2015110250A2 (en
Inventor
Markus Morrin
Hendrik Spahr
Gereon Hüttmann
Original Assignee
Euroimmun Medizinische Labordiagnostika Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Euroimmun Medizinische Labordiagnostika Ag filed Critical Euroimmun Medizinische Labordiagnostika Ag
Publication of WO2015110250A2 publication Critical patent/WO2015110250A2/en
Publication of WO2015110250A3 publication Critical patent/WO2015110250A3/en

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0056—Optical details of the image generation based on optical coherence, e.g. phase-contrast arrangements, interference arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B11/00—Measuring arrangements characterised by the use of optical techniques
    • G01B11/02—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness
    • G01B11/06—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness for measuring thickness ; e.g. of sheet material
    • G01B11/0616—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness for measuring thickness ; e.g. of sheet material of coating
    • G01B11/0675—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness for measuring thickness ; e.g. of sheet material of coating using interferometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B9/00—Measuring instruments characterised by the use of optical techniques
    • G01B9/02—Interferometers
    • G01B9/02041—Interferometers characterised by particular imaging or detection techniques
    • G01B9/02044—Imaging in the frequency domain, e.g. by using a spectrometer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B9/00—Measuring instruments characterised by the use of optical techniques
    • G01B9/02—Interferometers
    • G01B9/0209—Low-coherence interferometers

Abstract

Described is a method and an optical apparatus for creating an image of an object (103) located within a three-dimensional sample region (101), said method comprising the following steps: a) determining a depth profile (1375) of the sample region (101) at least at one lateral point (119) by means of optical low-coherence interferometry; b) identifying a peak (1353) in the depth profile resulting from the reflection off a point (120) on the object; c) determining an optical distance position (1354) of the peak (1353) representing the optical distance of the point (120) on the object from a reference point (136); d) calculating a geometrical distance position of the point on the object on the basis of the optical distance position (1354) and at least one previously known optical property of the sample region (101), said geometrical distance position representing a geometrical distance (gWI) of the point (120) on the object from the reference point (136); e) focusing a microscope (111) onto the point (120) on the object on the basis of the geometrical distance position (gWI) in order to create an image of the object (103).
PCT/EP2015/000034 2014-01-23 2015-01-12 Method for creating an image of an object, and optical apparatus WO2015110250A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP14000231.2 2014-01-23
EP14000231 2014-01-23
DE102014002584.5 2014-02-26
DE102014002584.5A DE102014002584A1 (en) 2014-01-23 2014-02-26 Method of imaging an obiect and optics device

Publications (2)

Publication Number Publication Date
WO2015110250A2 WO2015110250A2 (en) 2015-07-30
WO2015110250A3 true WO2015110250A3 (en) 2015-09-11

Family

ID=50033314

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2015/000034 WO2015110250A2 (en) 2014-01-23 2015-01-12 Method for creating an image of an object, and optical apparatus

Country Status (2)

Country Link
DE (1) DE102014002584A1 (en)
WO (1) WO2015110250A2 (en)

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DE102014217328A1 (en) * 2014-08-29 2016-03-03 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method and device for imaging in microscopy
DE102015117824B4 (en) * 2015-10-20 2017-06-29 Carl Zeiss Meditec Ag Apparatus and method for adjusting a focal distance in an optical observation device
DE102017105928A1 (en) 2017-03-20 2018-09-20 Carl Zeiss Microscopy Gmbh Microscope and method for imaging an object
EP3396430B1 (en) * 2017-04-27 2023-08-16 Euroimmun Medizinische Labordiagnostika AG Optical scanning arrangement and method
EP3418789A1 (en) * 2017-06-20 2018-12-26 Euroimmun Medizinische Labordiagnostika AG Method and microscope system for recording an image
DE102017223014A1 (en) 2017-12-18 2019-06-19 Carl Zeiss Microscopy Gmbh Method for determining the thickness of a sample holder in the beam path of a microscope

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CN102589463B (en) * 2012-01-10 2014-01-15 合肥工业大学 Two-dimensional and three-dimensional integrated imaging measurement system

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DE10009217A1 (en) * 1999-03-16 2000-09-21 Zeiss Carl Fa Methods for optical determination of characteristic values of lens systems such as radius, thickness air gaps etc. by determining number of strip cycles that occur with changing wavelengths
GB2355310A (en) * 1999-09-28 2001-04-18 Ocular Sciences Ltd White light interferometer
DE10319182B4 (en) * 2003-04-29 2008-06-12 Carl Zeiss Jena Gmbh Method and arrangement for determining the focus position when imaging a sample
US7342641B2 (en) * 2005-02-22 2008-03-11 Nikon Corporation Autofocus methods and devices for lithography
DE102006027836B4 (en) 2006-06-16 2020-02-20 Carl Zeiss Microscopy Gmbh Microscope with auto focus device
DE102008041284B4 (en) * 2008-05-07 2010-05-27 Carl Zeiss Surgical Gmbh Ophthalmic surgical microscope system with OCT measuring device
DE102008029479A1 (en) * 2008-06-20 2009-12-24 Carl Zeiss Meditec Ag Short-coherence interferometry for distance measurement
ES2728379T3 (en) 2008-11-19 2019-10-24 Euroimmun Medizinische Labordiagnostika Ag Analysis procedures and devices for biological reactions between a liquid phase and a solid phase
DE102010033249A1 (en) 2010-08-03 2012-02-09 Carl Zeiss Microlmaging Gmbh Autofocus System
DE102010035104A1 (en) 2010-08-23 2012-04-05 Euroimmun Medizinische Labordiagnostika Ag Automatic focusing apparatus and method for low luminance microscopy microscopy
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Non-Patent Citations (2)

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Also Published As

Publication number Publication date
WO2015110250A2 (en) 2015-07-30
DE102014002584A1 (en) 2015-07-23

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