WO2015042950A1 - Ensemble de guidage de bulles, dispositif de culture de micro-organismes à haute densité et leur utilisation - Google Patents

Ensemble de guidage de bulles, dispositif de culture de micro-organismes à haute densité et leur utilisation Download PDF

Info

Publication number
WO2015042950A1
WO2015042950A1 PCT/CN2013/084707 CN2013084707W WO2015042950A1 WO 2015042950 A1 WO2015042950 A1 WO 2015042950A1 CN 2013084707 W CN2013084707 W CN 2013084707W WO 2015042950 A1 WO2015042950 A1 WO 2015042950A1
Authority
WO
WIPO (PCT)
Prior art keywords
bubble
guide
meandering
culture
plate
Prior art date
Application number
PCT/CN2013/084707
Other languages
English (en)
Chinese (zh)
Inventor
钟琦
Original Assignee
钟琦
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 钟琦 filed Critical 钟琦
Priority to PCT/CN2013/084707 priority Critical patent/WO2015042950A1/fr
Publication of WO2015042950A1 publication Critical patent/WO2015042950A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/18—Gas cleaning, e.g. scrubbers; Separation of different gases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/46—Removing components of defined structure
    • B01D53/54—Nitrogen compounds
    • B01D53/56—Nitrogen oxides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/46—Removing components of defined structure
    • B01D53/62—Carbon oxides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84—Biological processes
    • B01D53/85—Biological processes with gas-solid contact
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Definitions

  • Bubble guiding assembly high-density microbial culture device and application thereof
  • the present invention relates to the field of microbial culture techniques, and more particularly to a bubble guiding assembly, a high density microbial culture device, and the use of the device in microbial culture and waste gas treatment.
  • the main causes of modern air pollution are industrial exhaust gases from industrial operations of coal-fired oil-fired gas and excessive emissions of exhaust gases from a variety of oil-burning vehicles.
  • the main components include harmful carbon dioxide, nitrogen oxides, sulfur oxides and Carbon monoxide and harmless nitrogen and water vapor.
  • Excessive carbon dioxide in the atmosphere can cause a greenhouse effect and can make the seawater sour, which is the main cause of the deterioration of the world environment.
  • Nitrogen oxides and sulphur oxides do not cause greenhouse effects, but these gases can turn into acids when they are in contact with water. Acid rain is the formation of acid when these gases become water.
  • the microbial environmental protection system replaces the chemical reaction with the growth of microorganisms, and allows the microorganisms to effectively use the artificial environment provided for growth.
  • the sulfur oxides, nitrogen oxides and carbon dioxide in the exhaust gas are naturally soluble in water for the growth of microorganisms, and the microbial environmental protection system is used for both gas absorption and microbial culture.
  • the center of the microbial environmental protection system is biological carbon sequestration. Most of the microorganisms selected are various microalgae.
  • the cultivation methods can be divided into open and closed. Open cultivation mainly uses tracks and ponds, which has the advantages of low construction and use costs and ease of work.
  • gaseous nutrients as bubbles to the culture medium of the microorganisms, especially photosynthetic microorganisms such as algae, in the presence of solar radiation and carbon-rich gases such as carbon dioxide.
  • Growth and photosynthesis are carried out in a suitable nutrient culture medium, thereby increasing the supply of gaseous nutrients in the microbial culture solution to facilitate the growth of microorganisms.
  • the third is to increase the height of the microbial culture solution experienced by the bubbles in the incubator.
  • the disadvantages of these three methods are: 1 will increase the energy consumption of the microbial culture process, thereby increasing the production cost; 2 can not enlarge the mode of the microbial incubator to the scale of industrial production; 3 can not use the microbial culture with only a small amount of microbial culture solution The culture is limited, and the application of the microbial incubator is limited. 4 For microalgae cultivation, the prior art can only provide carbon dioxide gas and light energy to a small part of microalgae cells for photosynthesis, thereby reducing the culture efficiency.
  • the object of the present invention is to provide a bubble guiding assembly and a high-density micro-culture device and application thereof which are low in energy consumption, high in efficiency, and modularly assembled, in view of the problems and deficiencies of the prior art.
  • a bubble guiding assembly comprising at least two meandering guiding members arranged in a horizontal direction; the meandering guiding member comprising at least two guiding plates which are sequentially connected up and down in a vertical direction, The guide plate is inclined relative to the horizontal plane And any adjacent guide plates of the same meandering guide member are opposite to the horizontal plane, and any adjacent curved guide members form a meandering passage; any guide plate of the meandering guide member and adjacent meandering The closest guide plates of the guide members overlap in the projected portion of the horizontal plane.
  • the bubble guiding assembly further includes a fixing member for fixing the meandering guiding member.
  • the bubble guiding assembly of the present invention greatly increases the area of bubble guiding per unit volume, and realizes modularization, can be easily assembled as needed, and can be applied to large-scale cultivation of microorganisms.
  • the guide plate is a flat plate or a curved plate or a wavy plate.
  • its angle with the horizontal plane is preferably 10-30.
  • the interval between the adjacent meandering guide members is 1 5 - 5 0 mm.
  • the present invention also provides a high-density microorganism culture apparatus comprising a container, a bubble generating device, at least one of the bubble guiding assemblies, and the bubble generating device and the bubble guiding assembly are disposed in the container The bubble generating device is disposed at the bottom of the bubble guiding assembly.
  • a light source such as an LED lamp, may also be disposed on the meandering guide member or on the inner wall of the container.
  • the present invention also provides the use of the high-density microbial culture apparatus in exhaust gas treatment, the method of exhaust gas treatment comprising: adding a culture liquid in a container of a high-density microbial culture apparatus and introducing a microorganism including carbon dioxide as a carbon source, a microorganism capable of metabolizing sulfur oxides, a microorganism capable of metabolizing nitrogen oxides, and a microorganism to be cultured, and introducing the exhaust gas into the bubble generating device to form bubbles in the culture liquid, the bubbles from the bubble guiding assembly The bottom enters the channel and climbs along the lower surface of the deflector to provide gas nutrition to the microorganisms.
  • the invention also provides the application of the high-density microbial culture device in microalgae cultivation, comprising adding a culture liquid in a container of a high-density microbial culture device and introducing the microalgae into the container.
  • the special structure of the bubble guiding assembly greatly improves the area of the bubble guiding and realizes modular assembly.
  • the guide plate in the bubble guiding assembly provides a tortuous upward traveling path for the bubble carrying the nutrient gas required for the microbial culture process, so that the bubble rises vertically during the process of ascending along the lower surface of the guide plate.
  • the slower speed increases the horizontal movement, multiplies the contact area between the bubbles and the microbial culture medium per unit volume, and prolongs the gas-liquid contact time, so that the nutrient gas required for microbial growth can be sufficiently transmitted from the gas phase to Microbial culture solution is used for microbial growth, which greatly improves the material and energy utilization efficiency in the microbial culture process, thereby improving the culture efficiency and reducing the cultivation cost.
  • the tortuous upward movement process of the bubble increases the gas-liquid agitation frequency, which drives
  • the movement of the microbial culture solution uniformly transfers the nutrients required for the growth of the microorganisms to the entire microbial culture solution, which is advantageous for improving the cultivation efficiency of the microorganisms, and the design and assembly method of the bubble guiding assembly is flexible, and the modularization and the adaptability are strong.
  • the high-density microbial culture device is applied to waste gas treatment and microalgae cultivation, and the production capacity can reach the level of industrial production.
  • Figure 1 is a schematic view showing the structure of a plurality of bubble guiding members when the guide plates of the meandering guide members are a plurality of;
  • Figure 2 is an exploded perspective view of the assembly of the bubble guiding assembly when the guiding plate is a square plate;
  • Figure 3 is a schematic structural view of the assembly of the bubble guiding assembly when the guiding plate is a square plate;
  • Figure 4 is a view of the bubble guiding assembly when the guide plate of the meandering guide member is two flat plates
  • Figure 5 is a schematic view showing the structure of the bubble guiding member when the guide plates of the meandering guide member are two flat plates;
  • Figure 6 is a schematic structural view of the assembly of the bubble guiding assembly when the guiding plate is a curved plate;
  • Figure 8 is a schematic view showing the structure of the bubble guiding assembly adopting another assembling manner
  • Figure 9 is a partial enlarged view A of Figure 8;
  • Figure 10 is a schematic structural view of a meandering guide member in the bubble guiding assembly of Figure 8;
  • Figure 11 is a schematic cross-sectional view of a high-density microbial culture device according to an embodiment of the present invention;
  • Figure 12 is a structural decomposition of a high-density microbial culture device according to an embodiment of the present invention;
  • BRIEF DESCRIPTION OF THE DRAWINGS Fig. 13 is a perspective view of a high-density microorganism culture apparatus according to an embodiment of the present invention.
  • Figures 1 - 5 show a preferred embodiment of the mass production of the convenient guide plates and the assembly of the bubble guide assembly.
  • the bubble guiding assembly 1 includes a plurality of meandering guiding members 10 arranged at equal intervals in a horizontal direction, and the meandering guiding members 10 include a plurality of guiding guides which are sequentially connected up and down in the vertical direction.
  • the plate 101 and the guide plate 101 are inclined with respect to the horizontal plane.
  • any adjacent guide plates 101 in the same meandering guide member 10 is opposite to the horizontal plane, and any adjacent zigzag guide members 10 form a meandering passage between the adjacent zigzag guide members 10, and the meandering Any of the guide plates 101 of the guide member 10 overlaps with the projected portion of the nearest guide plate 101 of the adjacent meander guide member 10 at the horizontal plane, and the air bubbles enter the passage from the bottom of the bubble guide assembly and climb along the lower surface of the guide plate 101.
  • the assembly method of the bubble guiding assembly 1 is to fix a plurality of guiding plates 101 arranged in parallel in parallel in the horizontal direction to obtain an assembly, and then superimpose the bubble guiding assembly 1 in the vertical direction, specifically flipping the assembly 180. ° , get another assembly, will The assembly before and after the flipping is superimposed, so that the guide plates 101 are in contact with each other and are inclined opposite to the horizontal plane, and the bubble guiding assembly 1 is obtained, which can be further fixed after being superposed to prevent the guiding plate from moving.
  • the fitting includes a guide plate 101 and a fixing member, and the fixing member includes a horizontal strip 111 and a wedge-shaped socket 112 having an oblique slot 1110 at an acute angle to the horizontal direction, and further includes a fastener 113 having a wedge-shaped socket 112 thereon
  • the groove 111 corresponds to the groove 1120.
  • the thickness of the wedge-shaped cap 112 is the same as the vertical height of the guide plate 101, and the surface adjacent to the guide plate 101 is parallel to the guide plate 101.
  • FIG 3 is a schematic structural view of the assembly of the bubble guiding assembly when the guiding plate is a square flat plate, and the guiding plate 101 is fixed by the horizontal strip 111 at the front and the rear of the guiding plate 101 which are equally spaced in a plurality of horizontal directions, at the end
  • the wedge-shaped platform 112 is disposed adjacent to the guide plates 101 at both ends, so that a channel is formed between the inclined surface of the wedge-shaped platform 112 and the guide plate 101 at the end, and the two ends of the horizontal strip 111 are respectively inserted into the 1120 on the wedge-shaped platform 112.
  • the fastener 113 is screwed to obtain an assembly.
  • Fig. 4 is a schematic view showing the decomposition of the bubble guiding assembly when the guide plates of the meandering guide member are two flat plates
  • Fig. 5 is a structural schematic view of the corresponding bubble guiding assembly.
  • the bubble guiding assembly is obtained by superimposing two assembly parts. The exploded view and structure of the assembly are shown in Fig. 2 and Fig. 3, and one assembly is flipped 180. Superimposed on another assembly to obtain the bubble guiding assembly 1 of Fig. 5.
  • the bubble guiding assembly of Fig. 1 can be assembled by a plurality of bubble guiding assemblies of Fig. 5.
  • the meandering guiding member 10 is composed of two guiding plates 101.
  • any adjacent zigzag guiding members 10 form a meandering passage 2, which is meandering.
  • Any of the guide plates 101a of the guide members 10 partially overlaps the projection of the closest guide plates 101b and the guide plates 101c of the adjacent meander guide members 10 in the horizontal plane.
  • the projection of the guide plate 101d of the meandering guide member 10 and the closest guide plate 101e of the adjacent meandering guide member 10 and the projection of the guide plate 101f in the horizontal plane also partially overlap.
  • the bubble 3 is in the channel 2, and climbs to the end along the lower surface of the guide plate 101d.
  • FIG. 6 is a schematic structural view of the assembly of the bubble guiding assembly when the guiding plate is a curved plate, the assembly includes the guiding plate 101 and the fixing member, and the structure of the fixing member and the assembling manner of the assembly are referred to FIG. 2 and FIG.
  • the guide plate 101 adopts a curved plate to further lengthen the upward path length of the bubble.
  • Fig. 7 is a schematic view showing the structure of the bubble guiding assembly when the guide plate is a curved plate, and the assembly of Fig. 6 is turned over 180.
  • the guiding plate 101 is brought into contact to obtain the bubble guiding assembly 1, including the meandering guiding member 10 and the meandering channel 2, and the air bubbles alternately along the guiding plates of the adjacent two zigzag guiding members 10
  • the lower surface of the 101 zigzag climbs up.
  • the guiding plate 101 on the meandering guiding member 10 is connected by contact, that is, the guiding plate 101 is independently detachable, so that the guiding plates 101 can be arranged in the horizontal direction.
  • the assembled and fixed assembly method is adopted to realize modular assembly, and the assembly method is flexible and convenient.
  • the guide plates 101 are arranged at equal intervals in the horizontal direction, and are parallel to each other. While increasing the density of the guide plates 101 and the gas-liquid contact area per unit volume, the spacing between the adjacent guide plates on the adjacent zigzag guide members is smaller. The larger the area of the overlapping portion of the projection on the horizontal surface, the longer the upward path of the bubble on the lower surface of the guide plate, and the larger the gas-liquid contact area.
  • Fig. 8 is a structural schematic view of a bubble guiding assembly adopting another assembling manner
  • Fig. 9 is a partial enlarged view A of Fig. 8.
  • the guiding plate 101 is inclined with respect to a horizontal plane, and the plurality of guiding plates 101 are formed in a vertical direction to form a meandering guiding member 10 in an integrated manner, and any adjacent guiding of the same zigzag guiding member 10
  • the plate 101 is oppositely inclined with respect to the horizontal plane, and a plurality of meandering guide members 10 are disposed at equal intervals in the horizontal direction, and are fixed by the fixing members.
  • the fixing member includes a card strip 1 14 disposed at both ends of the zigzag guiding member 10 and has a card
  • the strips 114 of the slot 114 of the slot 114 are tightly fitted, and the plurality of meandering guide members 10 are fixed by the rails 115 to obtain the bubble guiding assembly 1.
  • Figure 10 is a schematic view showing the structure of the meandering guide member 10 in the bubble guiding assembly 1 of Figure 8, which shows the positional relationship of the adjacent meandering guiding members 10, that is, the zigzag rise between any adjacent zigzag guiding members 10
  • any one of the guide plates 101 of the meandering guide member 10 overlaps with the projected portion of the nearest guide plate 101 of the adjacent meandering guide member 10 in the horizontal plane, so that the air bubbles alternately follow the guide plates 101 of the adjacent two meandering guide members 10.
  • the surface of the bubble guiding member 10 is climbed upward, and the upper and lower ends of the bubble guiding member 10 are respectively provided with the strips 1 14 for convenient fixing.
  • the bubble guiding assembly 1 in Fig. 8 adopts a processing method in which the guiding plates 101 are first fixedly connected in series or a plurality of guiding plates 101 are integrally molded at a time, that is, the guiding plates are self-contained, and after the meandering guiding members 10 are obtained, The plurality of meandering guide members 10 are combined and fixedly assembled, which facilitates the production of the meandering guide members 10 and is flexible in assembly.
  • the guide plates 101 are equally spaced in the horizontal direction and parallel to each other, which can increase the density of the guide plates 101 per unit volume and the gas-liquid contact area, and the smaller the pitch of the adjacent zigzag guide members, the overlapping projection of the guide plates on the horizontal plane. The larger the partial area, the longer the upward path of the bubble on the lower surface of the guide plate, and the larger the gas-liquid contact area.
  • the guiding plate and the meandering guiding member are fixed by the fixing member, and the fixing member and the fixing manner are various, and the guiding plate and the meandering guiding member are fixed, and the traveling path and the cultivation of the bubble zigzag upward are not affected.
  • the flow of liquid can be.
  • the guide plate in the bubble guiding assembly is a thin plate which cannot be penetrated by bubbles, and is made of plastic, metal or glass or other substances insoluble in the culture liquid.
  • it is preferably a light-transmitting plate.
  • the bubble is subjected to a vertical upward force or a bubble traveling speed in the culture solution, and when the impulse is large, the bubble can be pushed along the guide plate. Therefore, the specific shape of the guide plate and the inclination angle with respect to the horizontal plane are not strictly limited, and the air bubbles can be lifted up without being blocked, resulting in complete suspension, such as a wave shape, an arc shape or a plane, and a mixture of several shapes. Feasible, using curved surfaces such as curved plates or waves The wave plate can further increase the length of the bubble travel path.
  • the angle with the horizontal plane is preferably 10-30°, more preferably 20°.
  • the specific operation of the microbial culture may further include pretreatment such as preparation of the culture medium and conventional sterilization of the culture solution, and separation and purification after the completion of the culture.
  • microbial culture method comprises the following steps:
  • the microorganism to be cultured is the microalgae Synechococcus sp. MAI 9, and the medium BG11 is prepared by adding 1.5 g of sodium nitrate, 0.04 g of dipotassium hydrogen phosphate, and magnesium sulfate (MgS0 4 ⁇ 7 ⁇ 2 0 ).
  • the process of introducing the microalgae into the culture solution of the incubator is first pre-cultured, and the pre-culture includes the following steps:
  • the microalgae culture solution 3 in the three conical flasks was combined into a conical flask, and the medium BG11 to 2L was added to continue the culture.
  • the culture condition was the same as that of S01 to obtain a green turbid microalgae preculture solution. .
  • the pre-cultured microalgae preculture solution is added to the container of the high-density microbial culture device, and 18 L of the medium BG11 is added to open the LED lamp in the high-density microbial culture device, and the gas is continuously supplied and cultured.
  • the pre-culture and culture process of the microalgae is air containing 15% (by volume) of carbon dioxide, OOppm of nitrogen dioxide, and 250 ppm of sulfur dioxide.
  • the amount of intake air during the cultivation is 1 cubic meter per minute.
  • the microorganism culture method of the present invention can also be used for cultivating bacteria.
  • the preparation method of the culture medium LB is: tryptone l Og, yeast extract (yeas t extract) 5 g, chlorine Sodium (NaCl) 10g, agar powder 15_20g, dissolved in l OOOmL double-distilled water, using a 5mol / L NaOH solution about 0. 2ml to adjust the pH to 7.2, then sterilized at 121 °C for 30min after use.
  • the bacteria to be cultured E. col i was introduced into the medium LB in the culture vessel, and the gas was introduced and cultured.
  • FIG. 11 is a schematic cross-sectional view of a high-density microbial culture device
  • FIG. 12 is a schematic exploded view of a high-density microbial culture device
  • FIG. 13 is a perspective view of a high-density microbial culture device including a container and a container disposed therein.
  • the container is specifically an incubator, and includes a box body 41, a box cover 42 and an air outlet hole 43.
  • the bottom surface of the box body 41 is about 1 X lm 2 and the height is about lm.
  • the liquid level of the culture liquid in the incubator is higher than the bubble guide assembly 1 and the box.
  • the body 41 and the cover 42 are respectively made of plastic or metal or glass or other materials, and are not particularly limited.
  • the gas introduction device includes a gas distributor 51 and an intake duct 52 through which the gas passes through the intake duct 52. After being introduced into the air chamber at the bottom of the container, bubbles having a smaller diameter are generated in the culture liquid through the bubble generating device 6, and are entered from the bottom of the bubble guiding unit 1, and the bubbles 3 are overflowed after being exchanged with the culture liquid containing the microorganism to be cultured. The liquid is discharged from the air outlet 43.
  • the bubble generating device 6 is a microplate, and a gasket 7 for sealing is provided between the gas chamber and the bubble generating device.
  • the bubble generating device may also be a microporous tube or a microporous aerator or other device that can form a gas bubble in the culture solution.
  • the guide plate is a square transparent plate with a length of 1 m, a width of 10.6 cm and a thickness of l mm, and the angle with the horizontal plane is 20 degrees, and 28 (only a part of the figure is shown) are sequentially contacted vertically in the vertical direction.
  • the connected guide plates constitute a meandering guide member, and 45 (two parts are shown in the figure) are arranged at equal intervals (20 ⁇ ) in the horizontal direction.
  • the zigzag guide members are fixed to obtain a bubble guide assembly, and the bubbles alternate along two adjacent turns.
  • the lower surface of the guide plate of the guide member is bent up and up until the culture liquid containing the microorganism to be cultured escapes.
  • the bubble guiding assembly 1 After the bubble guiding assembly 1 is disposed in the casing 41, a gap 8 is left between the bubble guiding assembly 1 and the inner wall of the casing 41, and the culture liquid containing the microorganism to be cultured by the bubble is flowed to promote the substance in the culture system.
  • the equilibrium distribution increases the culture efficiency. Specifically, during the cultivation process, the bubbles travel along the meandering path in the channel of the bubble guiding assembly 1, and the culture liquid containing the microorganism to be cultured also moves upward, and then proceeds with the bubble.
  • the bubble guiding assembly 1 is returned to the bottom of the casing 41 along the gap 8 between the casing 41 and the bubble guiding assembly 1.
  • an LED lamp is mounted on the guide plate of the bubble guiding assembly 1 to project light around the bubble, so that the microorganism to be cultured can simultaneously obtain carbon dioxide and light energy necessary for photosynthesis, so that the microorganism to be cultured can effectively perform photosynthesis. Function and rapid growth.
  • the bubbles are cultured with microorganisms to be cultured.
  • the length of the travel path in the liquid is 2.99m
  • the relative vertical travel distance (lm) is increased by nearly 200%
  • the contact area of the bubble 3 with the microalgae-containing culture solution is 1 31 ⁇ 2 2 , which is more than when it is directly in the vertical direction.
  • the area lm 2 is increased by 34 times, which significantly increases the gas-liquid contact area per unit volume, and promotes gas-liquid material exchange and microbial culture.
  • the bubble drives the culture liquid containing the microalgae in the process of the zigzag upward movement, increases the gas-liquid agitation frequency, promotes the material balance, and improves the microbial culture efficiency.
  • the microorganisms to be cultured continue to divide and grow, the light transmittance of the culture liquid is drastically reduced, and the microorganisms are mainly grown only in a region where light can be received, and the superiority of such a device is fully manifested.
  • the growth of the microalgae is measured by observing the green change and detecting the change of the optical density of the culture liquid.
  • the test results show that the device can be used to culture the microalgae, and can process 297 g of carbon dioxide per minute, 0. 26 g. Nitrogen dioxide, 0.66 grams of sulfur dioxide, and 162 grams of microalgal biomass, significantly improved culture efficiency.

Abstract

La présente invention concerne un ensemble de guidage de bulles, comprenant au moins deux éléments de guidage en zigzag agencés dans la direction horizontale, dans lequel chaque élément de guidage en zigzag comprend au moins deux plaques de guidage séquentiellement raccordées au sommet et à la base dans la direction verticale; chaque plaque de guidage étant disposée obliquement par rapport au plan horizontal; les directions obliques de plaques de guidage adjacentes quelconques par rapport au plan horizontal dans le même élément de guidage en zigzag sont opposées; un canal d'élévation en zigzag est formé entre des éléments de guidage en zigzag adjacents quelconques; et l'une quelconque des plaques de guidage dans un élément de guidage en zigzag chevauche la partie saillante de la plaque de guidage la plus proche d'un élément de guidage en zigzag adjacent dans le plan horizontal. L'ensemble de guidage de bulles de la présente invention augmente fortement la surface de guidage de bulles par unité de volume et constitue un ensemble modulaire avec une grande adaptabilité. La présente invention concerne en outre un dispositif pour cultiver des micro-organismes à haute densité qui est capable de permettre une production à grande échelle et son utilisation dans le traitement de gaz d'échappement et les cultures de micro-algues.
PCT/CN2013/084707 2013-09-30 2013-09-30 Ensemble de guidage de bulles, dispositif de culture de micro-organismes à haute densité et leur utilisation WO2015042950A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2013/084707 WO2015042950A1 (fr) 2013-09-30 2013-09-30 Ensemble de guidage de bulles, dispositif de culture de micro-organismes à haute densité et leur utilisation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2013/084707 WO2015042950A1 (fr) 2013-09-30 2013-09-30 Ensemble de guidage de bulles, dispositif de culture de micro-organismes à haute densité et leur utilisation

Publications (1)

Publication Number Publication Date
WO2015042950A1 true WO2015042950A1 (fr) 2015-04-02

Family

ID=52741877

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2013/084707 WO2015042950A1 (fr) 2013-09-30 2013-09-30 Ensemble de guidage de bulles, dispositif de culture de micro-organismes à haute densité et leur utilisation

Country Status (1)

Country Link
WO (1) WO2015042950A1 (fr)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5417938A (en) * 1988-09-02 1995-05-23 Sulzer Brothers Limited Device for carrying out catalyzed reactions
US20070289206A1 (en) * 2006-06-14 2007-12-20 Malcolm Glen Kertz Method and apparatus for co2 sequestration
CN101171074A (zh) * 2005-05-05 2008-04-30 伊士曼化工公司 用于垂直分级的聚合反应器的导流板组件模块
US20110318804A1 (en) * 2010-05-21 2011-12-29 Karlsruher Institut Fuer Technologie Photobioreactor
CN102492609A (zh) * 2011-12-13 2012-06-13 天津大学 导流板和使用该导流板的平板式生物反应器及设置方法
CN103224875A (zh) * 2013-04-28 2013-07-31 钟琦 一种微生物培养装置
CN103525688A (zh) * 2013-09-30 2014-01-22 钟琦 一种气泡导向组件、高密度微生物培养装置及其应用
CN203613188U (zh) * 2013-09-30 2014-05-28 钟琦 一种气泡导向组件及其高密度微生物培养装置

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5417938A (en) * 1988-09-02 1995-05-23 Sulzer Brothers Limited Device for carrying out catalyzed reactions
CN101171074A (zh) * 2005-05-05 2008-04-30 伊士曼化工公司 用于垂直分级的聚合反应器的导流板组件模块
US20070289206A1 (en) * 2006-06-14 2007-12-20 Malcolm Glen Kertz Method and apparatus for co2 sequestration
US20110318804A1 (en) * 2010-05-21 2011-12-29 Karlsruher Institut Fuer Technologie Photobioreactor
CN102492609A (zh) * 2011-12-13 2012-06-13 天津大学 导流板和使用该导流板的平板式生物反应器及设置方法
CN103224875A (zh) * 2013-04-28 2013-07-31 钟琦 一种微生物培养装置
CN103525688A (zh) * 2013-09-30 2014-01-22 钟琦 一种气泡导向组件、高密度微生物培养装置及其应用
CN203613188U (zh) * 2013-09-30 2014-05-28 钟琦 一种气泡导向组件及其高密度微生物培养装置

Similar Documents

Publication Publication Date Title
Assunção et al. Enclosed “non-conventional” photobioreactors for microalga production: A review
Li et al. Efficiency of CO2 fixation by microalgae in a closed raceway pond
Hsueh et al. A batch study on the bio-fixation of carbon dioxide in the absorbed solution from a chemical wet scrubber by hot spring and marine algae
US8877486B2 (en) Photobioreactor for carbon dioxide mitigation in wastewater treatment
KR101148194B1 (ko) 투명 필름으로 이루어진 광합성 생물 반응기
ES2326296B1 (es) Fotobiorreactor vertical sumergible para la obtencion de biocombustibles.
CN102206570B (zh) 一种用于微藻规模培养的装置及培养方法
Chai et al. Enhanced removal of carbon dioxide and alleviation of dissolved oxygen accumulation in photobioreactor with bubble tank
CN111704990B (zh) 管池结合式闪光生物反应器系统及其微藻生长固碳方法
Lee et al. Enhanced carbon dioxide fixation of Haematococcus pluvialis using sequential operating system in tubular photobioreactors
CA2773794A1 (fr) Procede et systeme de traitement d'emissions gazeuses et d'effluents, et production de biomasse algale
CN103289888A (zh) 一种插板式微藻半干固态贴壁培养装置
Nayak et al. Improvement of carbon dioxide biofixation in a photobioreactor using Anabaena sp. PCC 7120
Mohapatra et al. Bio-inspired CO2 capture and utilization by microalgae for bioenergy feedstock production: A greener approach for environmental protection
Kumar et al. CO 2 sequestration through algal biomass production
Rinanti Biotechnology carbon capture and storage by microalgae to enhance CO2 removal efficiency in closed-system photobioreactor
Aslam et al. A review on microalgae to achieve maximal carbon dioxide (CO2) mitigation from industrial flue gases
CN109879409A (zh) 一种沼气提纯的膜生物反应器及其沼气提纯工艺系统
CN112961771A (zh) 用于空气净化的光生物反应器系统
AU2012379588B2 (en) Horizontal immersion cover type carbon supplement device and carbon supplement method used for microalgae culture in open pond
CN102676371B (zh) 一种固碳除氧光生物反应器及其应用
CN104560703A (zh) 用于空气净化的光生物反应器系统
CN101275117B (zh) 一种利用co2使藻类快速增殖直接用于生物制氢的方法
WO2015042950A1 (fr) Ensemble de guidage de bulles, dispositif de culture de micro-organismes à haute densité et leur utilisation
CN103525688A (zh) 一种气泡导向组件、高密度微生物培养装置及其应用

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13894508

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 13894508

Country of ref document: EP

Kind code of ref document: A1