WO2015011327A1 - Procédé d'inhibition de la croissance microbienne - Google Patents

Procédé d'inhibition de la croissance microbienne Download PDF

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Publication number
WO2015011327A1
WO2015011327A1 PCT/ES2014/070595 ES2014070595W WO2015011327A1 WO 2015011327 A1 WO2015011327 A1 WO 2015011327A1 ES 2014070595 W ES2014070595 W ES 2014070595W WO 2015011327 A1 WO2015011327 A1 WO 2015011327A1
Authority
WO
WIPO (PCT)
Prior art keywords
glass
microbial growth
inhibiting microbial
weight
biocidal
Prior art date
Application number
PCT/ES2014/070595
Other languages
English (en)
Spanish (es)
Inventor
José Serafín MOYA CORRAL
Ramón TORRECILLAS SAN MILLÁN
María Belén CABAL ÁLVAREZ
Eduardo ÁLVAREZ DÍAZ
Original Assignee
Consejo Superior De Investigaciones Científicas (Csic)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior De Investigaciones Científicas (Csic) filed Critical Consejo Superior De Investigaciones Científicas (Csic)
Publication of WO2015011327A1 publication Critical patent/WO2015011327A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/14—Boron; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/02—Inorganic materials
    • A61L27/10—Ceramics or glasses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • C—CHEMISTRY; METALLURGY
    • C03—GLASS; MINERAL OR SLAG WOOL
    • C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
    • C03C3/00—Glass compositions
    • C03C3/04—Glass compositions containing silica
    • C03C3/062—Glass compositions containing silica with less than 40% silica by weight
    • C03C3/064—Glass compositions containing silica with less than 40% silica by weight containing boron
    • C03C3/068—Glass compositions containing silica with less than 40% silica by weight containing boron containing rare earths
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
    • A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents

Definitions

  • the present invention is related to a process for preventing the growth of microorganisms through the use of inorganic materials, and more specifically of compounds that include boron.
  • the invention is related to the use of said method in the manufacture of packages, drugs, medical devices, surgical implants, tissues, means of transport, air installations or water installations.
  • the antimicrobial capacity of these glasses is mainly due to the increase in pH and osmotic pressure of the medium caused by the leaching of sodium, calcium, phosphate and silicate ions, although the disturbing effect caused by the high concentration of ions is also known. alkalis leached in the membrane potential and therefore may have consequences on the antimicrobial capacity of biovidrios.
  • sodoccalcic glasses have been described in which the biocidal activity is associated with the presence in calcium oxide (CaO) glass [ES2361695 of 04/27/2012].
  • CaO calcium oxide
  • the inventors establish a minimum content of 10% by weight of CaO as a requirement for the glass to exhibit biocidal activity.
  • biocidal activity of this sodoccalcic glass is attributed to the direct interaction between the glass particle and the cell membrane of the bacteria or fungus that would produce a partial or total interference with the metabolism of said bacterium or fungus.
  • glasses with antimicrobial properties characterized by containing metals such as copper or silver in the form of salts [patent ES2190732 of 06/16/2004], [patent US6921546 of 07/26/2005], [ patent application WO2005030665 of 07/04/2005] or as nanoparticles [Esteban-Tejeda L, Malpartida F, Esteban-Cubillo A, Pecharromán C, Moya JS Nanotechnology 25; 20 (8): 085103 (2009)], [Esteban-Tejeda L, Malpartida F, Esteban-Cubillo A, Pecharromán C, Moya JS, Nanotechnology 16; 20 (50): 505701 (2009)].
  • the activity in this type of glass is attributed to the presence of these metallic elements. It is known to use these ions or metal nanoparticles as bactericides, fungicides and algaecides.
  • a method has been developed that allows the inhibition of the growth of microorganisms in a lasting way by using a glass with a particular composition that includes a zinc oxide content greater than 15%.
  • the glass used in the invention does not require the presence of metals such as silver or copper and does not belong to the family of phosphate or sodoccalc glass.
  • glass is understood as an inorganic amorphous material and therefore has no crystalline phases.
  • Biocide means any synthetic or natural substance intended to destroy, counteract, neutralize, prevent the action or exert control of any other type on any microbial organism considered harmful to man, including bacteria, yeasts, fungi and viruses.
  • Bactericidal means any substance of natural or synthesized origin that is capable of destroying bacteria.
  • material is meant a substance with some useful property, whether mechanical, electrical, optical, thermal or magnetic, such as, for example, the material can be or be part of a metallic, ceramic, agglomerated, textile, plastic or plastic fibers. polymeric materials or composite materials, among others.
  • the object of the invention consists in this way in a method for the inhibition of microbial growth characterized by the use of a glass added to a material, hereinafter glass of the invention, which does not contain any of the following elements: phosphorus, silver, copper and which includes in its composition at least silicon oxide (Si0 2 ), boron oxide (B 2 0 3 ), zinc oxide (ZnO) with a zinc oxide content exceeding 15% by weight and calcium oxide with a content less than 10% by weight.
  • Si0 2 silicon oxide
  • B 2 0 3 boron oxide
  • ZnO zinc oxide
  • a preferred aspect of the invention is the presence in the glass used for the inhibition of microbial growth of the invention of a percentage of zinc oxide less than 55% by weight.
  • the glass of the invention has a silicon oxide content of between 16% and 30% by weight. In an even more preferred embodiment, the glass of the invention does not contain calcium oxide or contains traces (less than 0.1% by weight).
  • a preferred aspect of the invention consists in the use of the glass of the invention in particulate, fiber or sphere form.
  • a particular embodiment consists in incorporating the glass of the invention as a filler material in polymeric materials.
  • the biocidal effect of coatings made with the glass of the invention has been verified. Therefore, another particular embodiment is the use for biocidal purposes of coatings formed with the glass of the invention.
  • the method object of the invention can be used in various applications.
  • this method for the inhibition of microbial growth can be applied to packages, drugs, medical devices, surgical implants, tissues, means of transport (for example for devices or structural elements), air installations (for example for devices or structural elements , such as air purification filters) or water installations (for example for devices or structural elements, such as water purification filters).
  • Example 1 Test of biocidal activity of the glass of the invention in particulate form
  • a glass of chemical composition (% weight): 4.97% AI2O3 was prepared; 5.38% Na 2 0; 18.7% of Si02; 0, 10% of K 2 0; 0.17% Ti0 2 ; 34.7% ZnO; 0.0024% SrO; 33.2% of B 2 0 3 ; 0.45% of Ce0 2 ; 2.25% of Zr0 2 and 0.056% of others.
  • the bacteria were seeded in solid medium, Petri dishes, from Luria Bertani (LB) whose composition is (% weight): 1% tryptone, 0.5% yeast extract, 1% NaCl and 1.5% agar. The plates were incubated 18 hours at 37 ° C.
  • Yeasts in solid medium, Petri dishes of (YEPD) whose composition is (% weight): Bacto-pectone 1%, yeast extract 1%, glucose 2% and agar 2%. The plates were incubated 18 hours at 37 ° C.
  • colonies isolated from the above plates were inoculated in 1 mL of LB or liquid YEPD and cultured at 37 ° C for 6 hours to obtain precultures at a density of between 10 7 to 10 8 viable colonies per milliliter of culture.
  • a 200 mg / mL suspension in glass powder water will be prepared and sterilized for 30 minutes at 125 ° C.
  • 10 ⁇ _ of the bacterial preculture was inoculated in 1 ml_ of LB
  • 10 ⁇ _ of the yeast preculture was inoculated in 1 ml_ of YEPD.
  • 75 ⁇ _ of the glass suspension to be tested was added to this culture.
  • the same medium in which the amount of glass was replaced by an equivalent volume of water was prepared as a control.
  • the different cultures were incubated in horizontal agitation, making extractions every 24 hours in order to proceed to title the surviving microorganisms by plating in solid LB medium by serial dilutions from 10 ⁇ 1 to 10 ⁇ 8 .
  • Example 2 Biocidal activity test of the glass of the invention applied as a coating on titanium alloys
  • the coatings were carried out with the particulate biocide glass described in example 1, on plates of the titanium alloy Ti- 6AI-4V, of dimensions 12.5 x 8.3 x 1 mm and with 99.0 % purity
  • antimicrobial efficacy was determined following the procedure described in ISO 22196. This standard has been adopted internationally as a reference standard to evaluate the antimicrobial efficacy of non-porous, ceramic, plastic surfaces, etc. According to this standard, antimicrobial activity is determined by comparing the results obtained between the treated surface and a control surface, without biocidal agent (blank), after an incubation period of 48 hours at 37 ° C, optimum temperature for the growth of the selected microorganism.
  • ISO 22196 defines the antimicrobial activity of a surface as the difference between the number of bacteria in the control sample (blank, A) and the number of bacteria in the treated sample (B), according to the equation:
  • Example 3 Biocidal activity test of a polymer which contains the glass of the invention in particulate form as filler material
  • thermoplastic polymer polyurethane was selected as the polymer.
  • filler material a particulate glass similar to that described in example 1 was selected.
  • a weight load of 50% biocidal glass was added. Homogeneous mixing of all the members of the formulation was performed hot. Using an extruder, the formulation was obtained in the form of threads, which were subsequently processed in the form of sheets by using a hot plate hydraulic press. Similarly, controls were obtained containing only the polymer without biocide additive.
  • bactericidal tests were performed against the Gram-negative Escherichia coli DH10B bacteria.
  • the value of antimicrobial efficacy was determined following the procedure described in ISO 22196. The standard indicates that the bactericidal efficacy index must be greater than 2 to be considered as such. In all In the cases studied, the value obtained was greater than 5. This means a decrease in the number of bacteria greater than 99.999% compared to the initial population.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Dermatology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Geochemistry & Mineralogy (AREA)
  • Materials Engineering (AREA)
  • Organic Chemistry (AREA)
  • Ceramic Engineering (AREA)
  • Glass Compositions (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne un procédé d'inhibition de la croissance microbienne, comprenant l'utilisation d'un verre dont la composition inclut divers oxydes d'éléments tels que calcium, zinc, silice et bore. L'invention concerne également l'utilisation dudit procédé pour la fabrication d'emballages, de médicaments, de dispositifs médicaux, d'implants chirurgicaux, de tissus, de moyens de transport, d'installations à circulation d'air ou d'installations à circulation d'eau.
PCT/ES2014/070595 2013-07-23 2014-07-23 Procédé d'inhibition de la croissance microbienne WO2015011327A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP201331112 2013-07-23
ES201331112A ES2529557B1 (es) 2013-07-23 2013-07-23 Procedimiento para la inhibición del crecimiento microbiano

Publications (1)

Publication Number Publication Date
WO2015011327A1 true WO2015011327A1 (fr) 2015-01-29

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PCT/ES2014/070595 WO2015011327A1 (fr) 2013-07-23 2014-07-23 Procédé d'inhibition de la croissance microbienne

Country Status (2)

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ES (1) ES2529557B1 (fr)
WO (1) WO2015011327A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4098610A (en) * 1977-05-23 1978-07-04 Corning Glass Works Biocidal glass additive for marine paints
JPH1129343A (ja) * 1997-07-07 1999-02-02 Nippon Electric Glass Co Ltd 抗菌性ガラス粉末及び樹脂成形体
US6410633B1 (en) * 1997-08-20 2002-06-25 Nippon Electric Glass Co., Ltd. Antibacterial glass and resin composite comprising the same
US20060142413A1 (en) * 2003-02-25 2006-06-29 Jose Zimmer Antimicrobial active borosilicate glass

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4098610A (en) * 1977-05-23 1978-07-04 Corning Glass Works Biocidal glass additive for marine paints
JPH1129343A (ja) * 1997-07-07 1999-02-02 Nippon Electric Glass Co Ltd 抗菌性ガラス粉末及び樹脂成形体
US6410633B1 (en) * 1997-08-20 2002-06-25 Nippon Electric Glass Co., Ltd. Antibacterial glass and resin composite comprising the same
US20060142413A1 (en) * 2003-02-25 2006-06-29 Jose Zimmer Antimicrobial active borosilicate glass

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 199916, 6 October 2014 Derwent World Patents Index; Class D22, AN 1999-183564 *

Also Published As

Publication number Publication date
ES2529557A1 (es) 2015-02-23
ES2529557B1 (es) 2015-12-02

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