WO2015006843A3 - Bifunctional enzyme used to degrade biomass and produce xylose in a single operation - Google Patents

Bifunctional enzyme used to degrade biomass and produce xylose in a single operation Download PDF

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Publication number
WO2015006843A3
WO2015006843A3 PCT/BR2014/000218 BR2014000218W WO2015006843A3 WO 2015006843 A3 WO2015006843 A3 WO 2015006843A3 BR 2014000218 W BR2014000218 W BR 2014000218W WO 2015006843 A3 WO2015006843 A3 WO 2015006843A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
enzyme used
relates
single operation
bifunctional enzyme
Prior art date
Application number
PCT/BR2014/000218
Other languages
French (fr)
Portuguese (pt)
Other versions
WO2015006843A2 (en
Inventor
Roberto RULLER
José Alberto DIOGO
Zaira Bruna HOFFMAM
Junio Cota SILVA
Fabio Marcio SQUINA
José Geraldo da Cruz PRADELLA
Original Assignee
Centro Nacional De Pesquisa Em Energia E Materiais - Cnpem
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro Nacional De Pesquisa Em Energia E Materiais - Cnpem filed Critical Centro Nacional De Pesquisa Em Energia E Materiais - Cnpem
Publication of WO2015006843A2 publication Critical patent/WO2015006843A2/en
Publication of WO2015006843A3 publication Critical patent/WO2015006843A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a new bifunctional enzyme with xylanase and xylosidase activity that is produced from Bacillus subtilis and catalyses hemicellulose degradation. The invention is also directed to a sequence of nucleic acids that codes for the enzyme, and to a xylose preparation method by hydrolysis of biomass, preferably sugar cane bagasse or straw. The present invention further relates to a method for treating a liquor rich in hemicellulose from sugar cane bagasse preferably treated with hot water or by steam-explosion. In addition, the invention also relates to an enzyme used to supplement other enzyme cocktails, for example commercially available cocktails, in order to improve the performance thereof.
PCT/BR2014/000218 2013-07-15 2014-07-02 Bifunctional enzyme used to degrade biomass and produce xylose in a single operation WO2015006843A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRBR102013018051-3A BR102013018051A2 (en) 2013-07-15 2013-07-15 Bifunctional enzyme used in biomass degradation for xylose production in a single operation
BR102013018051-3 2013-07-15

Publications (2)

Publication Number Publication Date
WO2015006843A2 WO2015006843A2 (en) 2015-01-22
WO2015006843A3 true WO2015006843A3 (en) 2015-04-02

Family

ID=52346784

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2014/000218 WO2015006843A2 (en) 2013-07-15 2014-07-02 Bifunctional enzyme used to degrade biomass and produce xylose in a single operation

Country Status (2)

Country Link
BR (1) BR102013018051A2 (en)
WO (1) WO2015006843A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101870966A (en) * 2009-04-27 2010-10-27 复旦大学 Cellulose degrading enzyme with glucosidase/xylosidase dual functions and preparation method and application thereof
WO2011153516A2 (en) * 2010-06-03 2011-12-08 Mascoma Corporation Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
WO2012088467A2 (en) * 2010-12-22 2012-06-28 Mascoma Corporation Genetically modified clostridium thermocellum engineered to ferment xylose

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101870966A (en) * 2009-04-27 2010-10-27 复旦大学 Cellulose degrading enzyme with glucosidase/xylosidase dual functions and preparation method and application thereof
WO2011153516A2 (en) * 2010-06-03 2011-12-08 Mascoma Corporation Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
WO2012088467A2 (en) * 2010-12-22 2012-06-28 Mascoma Corporation Genetically modified clostridium thermocellum engineered to ferment xylose

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
LU P ET AL.: "Bifunctional enhancement of a beta-glucanase- xylanase fusion enzyme by optimization of peptide linkers.", APPL MICROBIOL BIOTECHNOL., vol. 79, no. 4, 2008, pages 579 - 87, XP019623621 *
PANBANGRED, W. ET AL.: "Expression of a xylanase gene of Bacillus pumilus in Escherichia coli and Bacillus subtilis.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 22, no. 4, 1985, pages 259 - 264 *
RIBEIRO, LF. ET AL.: "Engineering bifunctional laccase-xylanase chimeras for improved catalytic performance.", J BIOL CHEM., vol. 286, no. 50, 2011, pages 43026 - 43038 *
ZHANMIN, F. ET AL.: "Engineering of a multifunctional hemicellulase.", BIOTECHNOL LETT., vol. 31, no. 5, 2009, pages 751 - 7, XP019670307 *
ZHANMIN, F. ET AL.: "Multimeric hemicellulases facilitate biomass conversion.", APPL ENVIRON MICROBIOL., vol. 75, no. 6, 2009, pages 1754 - 1757 *
ZHANMIN, F. ET AL.: "The construction and characterization of two xylan-degrading chimeric enzymes.", BIOTECHNOL BIOENG., vol. 102, no. 3, 2008, pages 684 - 92 *

Also Published As

Publication number Publication date
BR102013018051A2 (en) 2015-09-01
WO2015006843A2 (en) 2015-01-22

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