WO2015003171A3 - Compositions, kits, and related methods for detecting and/or monitoring shiga toxin producing escherichia coli - Google Patents

Compositions, kits, and related methods for detecting and/or monitoring shiga toxin producing escherichia coli Download PDF

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Publication number
WO2015003171A3
WO2015003171A3 PCT/US2014/045485 US2014045485W WO2015003171A3 WO 2015003171 A3 WO2015003171 A3 WO 2015003171A3 US 2014045485 W US2014045485 W US 2014045485W WO 2015003171 A3 WO2015003171 A3 WO 2015003171A3
Authority
WO
WIPO (PCT)
Prior art keywords
escherichia coli
kits
detecting
compositions
monitoring
Prior art date
Application number
PCT/US2014/045485
Other languages
French (fr)
Other versions
WO2015003171A2 (en
Inventor
Michael Mcclellan Becker
Bettina GROSCHEL
Kristin Livezey
Original Assignee
Roka Bioscience, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roka Bioscience, Inc. filed Critical Roka Bioscience, Inc.
Priority to AU2014285024A priority Critical patent/AU2014285024A1/en
Priority to CA2916518A priority patent/CA2916518A1/en
Publication of WO2015003171A2 publication Critical patent/WO2015003171A2/en
Publication of WO2015003171A3 publication Critical patent/WO2015003171A3/en
Priority to US14/984,750 priority patent/US20160115527A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

The invention relates to a method for testing a sample for the presence of a pathogenic Escherichia coli, the method including detecting the presence of (i) ecf and (ii) wzx and/or stx in the sample, wherein detection of (i) ecf and (ii) wzx and/or stx in the sample is taken as an indication that the sample includes pathogenic E. coli.
PCT/US2014/045485 2011-12-30 2014-07-03 Compositions, kits, and related methods for detecting and/or monitoring shiga toxin producing escherichia coli WO2015003171A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2014285024A AU2014285024A1 (en) 2013-07-03 2014-07-03 Compositions, kits, and related methods for detecting and/or monitoring Shiga toxin producing Escherichia coli
CA2916518A CA2916518A1 (en) 2013-07-03 2014-07-03 Compositions, kits, and related methods for detecting and/or monitoring shiga toxin producing escherichia coli
US14/984,750 US20160115527A1 (en) 2011-12-30 2015-12-30 Compositions, kits, and related methods for detecting and/or monitoring shiga toxin producing escherichia coli

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361842924P 2013-07-03 2013-07-03
US61/842,924 2013-07-03

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
US14/369,888 Continuation-In-Part US20140342362A1 (en) 2011-12-30 2012-12-31 Compositions, kits, and related methods for detecting and/or monitoring shiga toxin producing escherichia coli
PCT/US2012/072329 Continuation-In-Part WO2013102218A1 (en) 2011-12-30 2012-12-31 Compositions, kits, and related methods for detecting and/or monitoring shiga toxin producing escherichia coli

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US14/984,750 Continuation US20160115527A1 (en) 2011-12-30 2015-12-30 Compositions, kits, and related methods for detecting and/or monitoring shiga toxin producing escherichia coli

Publications (2)

Publication Number Publication Date
WO2015003171A2 WO2015003171A2 (en) 2015-01-08
WO2015003171A3 true WO2015003171A3 (en) 2015-03-12

Family

ID=52144298

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/045485 WO2015003171A2 (en) 2011-12-30 2014-07-03 Compositions, kits, and related methods for detecting and/or monitoring shiga toxin producing escherichia coli

Country Status (3)

Country Link
AU (1) AU2014285024A1 (en)
CA (1) CA2916518A1 (en)
WO (1) WO2015003171A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111500596B (en) * 2020-04-30 2020-12-25 中国科学院华南植物园 Ephedra sinica gene CeSC20 and application thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113215290B (en) * 2020-02-05 2022-11-11 厦门大学 Method and kit for detecting serotype of shigella

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090170076A1 (en) * 2004-05-20 2009-07-02 Gregory Taylor Polynucleotides for the detection of escherichia coli 0157:h7 and escherichia coli 0157:nm verotoxin producers
US20120202204A1 (en) * 2009-08-11 2012-08-09 Patrick Fach Assay for Determining a Molecular Risk Assessment of a Complex Polymicrobial Sample Suspected to Contain an EHEC
US20130101591A1 (en) * 2006-04-20 2013-04-25 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Methods and compositions based on shiga toxin type 1 protein

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090170076A1 (en) * 2004-05-20 2009-07-02 Gregory Taylor Polynucleotides for the detection of escherichia coli 0157:h7 and escherichia coli 0157:nm verotoxin producers
US20130101591A1 (en) * 2006-04-20 2013-04-25 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Methods and compositions based on shiga toxin type 1 protein
US20120202204A1 (en) * 2009-08-11 2012-08-09 Patrick Fach Assay for Determining a Molecular Risk Assessment of a Complex Polymicrobial Sample Suspected to Contain an EHEC

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LIM ET AL.: "Phenotypic Diversity of Escherichia coli 0157:H7 Strains Associated With the Plasmid 0157", J MICROBIOL, vol. 48, no. 3, June 2010 (2010-06-01), pages 347 - 357 *
YOON ET AL.: "Involvement of the Escherichia coli O157:H7(pO157) ecf Operon and Lipid A Myristoyl Transferase Activity in Bacterial Survival in the Bovine Gastrointestinal Tract and Bacterial Persistence in Farm Water Troughs", INFECTION AND IMMUNITY, vol. 73, no. 4;, April 2005 (2005-04-01), pages 2367 - 2378 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111500596B (en) * 2020-04-30 2020-12-25 中国科学院华南植物园 Ephedra sinica gene CeSC20 and application thereof

Also Published As

Publication number Publication date
CA2916518A1 (en) 2015-01-08
AU2014285024A1 (en) 2016-01-21
WO2015003171A2 (en) 2015-01-08

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