WO2014210564A1

WO2014210564A1 - Glycan conjugates and use thereof

Info

Publication number: WO2014210564A1
Application number: PCT/US2014/044740
Authority: WO
Inventors: Chi-Huey Wong; Chung-Yi Wu; Chia-Hung Wang; Shiou-Ting LI
Original assignee: Academia Sinica
Priority date: 2013-06-27
Filing date: 2014-06-27
Publication date: 2014-12-31
Also published as: EP3013347A4; EP3013347A1; EP3013347B1; US9981030B2; US20160175421A1

Abstract

Described herein are synthetic glycan conjugates comprising a carrier and a glycan moiety derived from Neisseria meningitidis, wherein the glycan moiety is covalently linked to the carrier through a linker. Also provided herein are a mixture of the glycan conjugates thereof, immmunogenic compositions thereof, and kits thereof. The invention further provides methods of using the synthetic glycan conjugates and immunogenic compositions thereof to treat and/or reduce the risk of infectious diseases such as bacterial infections.

Description

GLYCAN CONJUGATES AND USE THEREOF

INVENTORS:

Chi-Huey WONG, Chung-Yi WU, Chia-Hung WANG and Shiou-Ting LI

CROSS-REFERENCE TO RELATED APPLICATION

[0001] This application claims priority of U.S. Provisional Patent Application Serial No. 61/840,324, Filed June 27, 2013 and titled "GLYCAN CONJUGATES AND USE THEREOF," the contents of which are incorporated herein in their entirety by reference.

FIELD OF INVENTION

[0002] The invention relates to meningococcal antigens and immunogenic compositions comprising such. In particular, the application relates to glycan conjugates comprising oligosaccharide antigens derived from Neisseria meningitidis and their application in therapy.

BACKGROUND OF THE INVENTION

[0003] Neisseria meningitidis (meningococcus) is a Gram negative human pathogen that causes meningococcal disease, including meningitis and bacterial septicemia induced shock that affect children and young adults ((a) Y. L. Tzeng, D. S. Stephens, Microbes Infect 2000, 2, 687- 700; b) M. Virji, Nat Rev Microbiol 2009, 7, 274-286). Vaccines for protecting against meningococcal disease include polysaccharide based vaccines, protein vaccines, and meningococcal outer membrane vesicle (OMV) vaccines.

[0004] Based on the surface capsular oligosaccharides of the organism, 13 serogroups of N. meningitidis have been identified, among which A, B, C, Y, and W135 are the major pathogenic strains (Ν. E. Rosenstein, B. A. Perkins, D. S. Stephens, T. Popovic, J. M. Hughes, N Engl J Med 2001, 344, 1378-1388; b) D. S. Stephens, FEMS Microbiol Rev 2007, 31, 3-14). Group A is the pathogen most often implicated in seasonal epidemic disease in developing countries of Asia and sub-Saharan Africa (M. Achtman, Trends Microbiol 1995, 3, 186-192). Serogroups B and C cause majority of the cases in industrial countries, such as United States of America and other developed countries (D. S. Stephens, B. Greenwood, P. Brandtzaeg, Lancet 2007, 369, 2196- 2210). Serogroups W135 and Y are responsible for the remaining cases in the developed countries.

[0005] The capsular polysaccharide plays an important role in the bacterial pathogenesis; its antiphagocytic properties help the bacteria to escape from antibody and complement deposition (a) M. R. Spinosa, C. Progida, A. Tala, L. Cogli, P. Alifano, C. Bucci, Infect Immun 2007, 75, 3594-3603; b) M. C. Schneider, R. M. Exley, S. Ram, R. B. Sim, C. M. Tang, Trends Microbiol 2007, 15, 233-240). On the other hand, the unique structures of the capsular polysaccharide also make a good target for vaccine design. Currently, the major source of polysaccharide for vaccine preparation is from acidic lysis of bacteria and column chromatography purification (A. Bardotti, G. Averani, F. Berti, S. Berti, C. Galli, S. Giannini, B. Fabbri, D. Proietti, N. Ravenscroft, S. Ricci, Vaccine 2005, 23, 1887-1899). Due to the limit of purification, the obtained

polysaccharide is heterogeneous; therefore, the vaccine quality is inconsistent. Therefore, there remains a need for developing capsular polysaccharides for homogeneous vaccines.

SUMMARY OF THE INVENTION

[0006] Neisseria meningitidis (meningococcus) has serogroups: A, B, C, Y and W135, according to the nature of the capsule polysaccharides. Because many bacterial polysaccharides are poor immunogens, development of vaccines based on bacterial capsule polysaccharides is often challenging.

[0007] The present invention is based on the unexpected discoveries that glycan conjugates comprising an oligosaccharide antigen derived from N. meningitidis and a carrier such as a toxin protein, successfully elicited immune responses specific to the oligosaccharide antigens.

[0008] Accordingly, the present invention provides synthetic glycan conjugates,

immmunogenic compositions comprising such, and kits thereof. The present invention further provides methods of using the synthetic glycan conjugates and immunogenic compositions thereof to treat or reduce the risk for infectious diseases (e.g., bacterial infections), such as infections caused by Neisseria meningitides (e.g., Neisseria meningitides serogroups W135).

[0009] In one aspect, the present invention provides a compound of Formula (F-l)

(F-l)

or a salt thereof, wherein each instance of R , R , R , R , R , R , and R is independently hydrogen, optionally substituted Ci_6 alkyl, or an oxygen protecting group; or optionally R¹ and R² are taken with the intervening atoms to form a heterocyclic ring; or optionally R² and R³ are taken with the intervening atoms to form a heterocyclic ring; or optionally R⁵ and R⁶ are taken with the intervening atoms to form a heterocyclic ring; or R^N1 and R⁰¹ are taken together with the intervening atoms to form a heterocyclic ring; each instance of R^N1 and R ² is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group; each instance of R⁰¹ is independently hydrogen, optionally substituted Ci_6 alkyl, or an oxygen protecting group; B is -N₃ or N(R^BN)₂; each instance of R^BN is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group; n is an integer of 1 to 100, inclusive; and p is an integer of 1 to 10, inclusive.

[0010] In one aspect, the present invention provides a compound of Formula (F-2)

(F-2)

or a salt thereof.

[0011] In one aspect, the present invention provides a glycan conjugate or a pharmaceutically acceptable salt thereof, a (I)

(I)

wherein: the glycan moiety is covalently linked to the carrier through a linker -L-; each instance of R¹, R², R³, R⁴, R⁵, R⁶, and R⁷ is independently hydrogen, optionally substituted Ci_6 alkyl, or an oxygen protecting group; or optionally R¹ and R² are taken with the intervening atoms to form a heterocyclic ring; or optionally R² and R³ are taken with the intervening atoms to form a heterocyclic ring; or optionally R⁵ and R⁶ are taken with the intervening atoms to form a heterocyclic ring; or optionally R⁶ and R⁷ are taken with the intervening atoms to form a heterocyclic ring; or R^N1 and R⁰¹ are taken together with the intervening atoms to form a heterocyclic ring; each instance of R^N1, R^N2, and R^BN is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group; each instance of R⁰¹ is independently hydrogen, optionally substituted Ci_6 alkyl, or an oxygen protecting group; each instance of L is a bond, -C(=0)-, -C(=0)NR^La- -C(=0)S- -C(=0)0- - C(=S)NR^La-, irara-CR^Lb=CR^Lb-, czs-CR^Lb=CR^Lb-, -C≡C-, C(R^Lb)₂0 , -C(R^Lb)₂NR^La-, - C(R^L )₂S-, -S(=0)₂0-, -S(=0)₂NR^La-, or an optionally substituted Ci_₃o hydrocarbon chain, optionally wherein one or more carbon units of the hydrocarbon chain is replaced with -0-, -S- , -NR^- -C(=0)-, NR^LaC(=0)-, -NR^LaC(=0)0- -C(=0)NR^La-, -OC(=0)NR^La-, -SC(=0)-, -C(=0)S-, -OC(=0)-, -C(=0)0-, -NR^LaC(=S)-, -C(=S)NR^La- trans-CR^Lb=CR^Lh- cis- CR^Lb=CR^Lb-, -C≡C-, -S(=0)₂0- -OS(=0)₂-, -S(=0)₂NR^La-, or -NR^LaS(=0)₂-, wherein R^La is hydrogen, optionally substituted Ci-is alkyl, or a nitrogen protecting group, or R^La is joined with the adjacent carbon atom to form an optionally substituted heterocyclic ring, and wherein each occurrence of R^Lb is independently selected from the group consisting of hydrogen, halogen, optionally substituted Ci-is alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl, or R^Lb is joined with the adjacent carbon or nitrogen or oxygen atom to form an optionally substituted carbocyclic or heterocyclic ring, or two R^Lb groups are joined to form an optionally substituted carbocyclic or optionally substituted heterocyclic ring; p is an integer of 1 to 10, inclusive; and n is an integer of 1 to 100, inclusive.

[0012]

(I-a)

wherein each instance of L¹ is a bond, -0-, -S-, -NR^Lla- -C(=0)-, -NR^LlaC(=0)- - NR^LlaC(=0)0- -C(=0)NR^Lla- -OC(=0)NR^Lla-, -SC(=0)-, -C(=0)S- -OC(=0)-, - C(=0)0-, -NR^LlaC(=S)-, -C(=S)NR^Lla-, ira«s-CR^Llb=CR^Llb-, c«-CR^Llb=CR^Llb-, -C≡C-, - OC(R^Llb)₂- -C(R^Llb)₂0- -NR^LlaC(R^Llb)₂-,— C(R^Llb)₂NR^Lla— , -SC(R^Llb)₂-, -C(R^Llb)₂S-, - S(=0)₂0-, -OS(=0)₂-, -S(=0)₂NR^Lla- -NR^LlaS(=0)₂-, or an optionally substituted Ci_₂o hydrocarbon chain, optionally wherein one or more carbon units of the hydrocarbon chain is replaced with -0-, -S-, -NR^Lla- -C(=0)-, NR^LlaC(=0)-, -NR^LlaC(=0)0-, -C(=0)NR^Lla-, - OC(=0)NR -, -SC(=0)-, -C(=0)S- -OC(=0)-, -C(=0)0-, -NR^LlaC(=S)-, -C(=S)NR^Lla- trans-CR^Llh=CR^Llh-, ds-CR^Llb=CR^Llb-, -C≡C-, -S(=0)₂0-, -OS(=0)₂-, -S(=0)₂NR^L1 -, or - NR^LlaS(=0)2-, wherein R^Ll is hydrogen, optionally substituted Ci_6 alkyl, or a nitrogen protecting group, or R^Lla is joined with the adjacent carbon atom to form an optionally substituted heterocyclic ring, and wherein each occurrence of R^Llb is independently selected from the group consisting of hydrogen, halogen, optionally substituted Ci_io alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl, or R^Llb is joined with the adjacent carbon or nitrogen or oxygen atom to form an optionally substituted carbocyclic or heterocyclic ring, or two R^Llb groups are joined to form an optionally substituted carbocyclic or optionally substituted heterocyclic ring; each instance of L² is a moiety derived from a crosslinkmg reagent capable of crosslinking the carrier and L¹; each

3C 1 instance of L is a crosslinking reagent capable of crosslinking the carrier and L ; each instance of R^CN is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group; w is a integer of 1 to 100, inclusive; and y is 0 or an integer of 1 to 100, inclusive.

[0

(I-b)

or a pharmaceutically acceptable salt thereof, wherein: each instance of p is an integer of 1 to 8, inclusive; and each instance of q is an integer of 1 to 8, inclusive.

[0014] In certain embodiments, the invention provides a glycan conjugate of Formula (I-c)

(I-c)

wherein each instance of R^8a, R^8b, and R⁹ is independently hydrogen, halogen, or optionally substituted Ci-e alkyl; and t is an integer of 1 to 8, inclusive.

[0015] In certain embodiments, the invention provides a glycan conjugate of Formula (I-d)

(I-d)

wherein each instance of R^Na is independently hydrogen, optionally substituted Ci_₆ alkyl, optionally substituted acyl, or a nitrogen protecting group; and i is an integer of 1 to 8, inclusive.

[0016] In certain embodiments, the invention provides a glycan conjugate of Formula (I-e)

(I-e) wherein each instance of R ^a is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group; each instance of q is an integer of 1 to 8, inclusive; and i is an integer of 1 to 8, inclusive.

[00

(I-f)

wherein each instance of q is an integer of 1 to 8, inclusive.

[0018] As generally defined herein, n is an integer of 1 to 100, inclusive. In certain

embodiments, n is an integer of 1 to 80, inclusive. In certain embodiments, n is an integer of 1 to 60, inclusive. In certain embodiments, n is an integer of 1 to 40, inclusive. In certain

embodiments, n is an integer of 1 to 20, inclusive. In certain embodiments, n is an integer of 1 to 10, inclusive. In certain embodiments, n is an integer of 1 to 8, inclusive. As generally defined herein, n is an integer of 1 to 8 inclusive. In certain embodiments, n is 1. In certain embodiments, n is 2. In certain embodiments, n is 3. In certain embodiments, n is 4. In certain embodiments, n is 5. In certain embodiments, n is 6. In certain embodiments, n is 7. In certain embodiments, n is 8.

[0019] In certain embodiments, w is an integer of 1 to 10, inclusive.

[0020] In certain embodiments, y is 0 or an integer of 1 to 10, inclusive.

[0021] In certain embodiments, p is 5.

[0022] In certain embodiments, R¹, R², R⁴, R⁵, R⁶, and R⁷ are hydrogen.

[0023] In certain embodiments, R^N1 is acetyl.

[0024] In certain embodiments, R^N2 is hydrogen.

[0025] In certain embodiments, R^BN is hydrogen.

[0026] In certain embodiments, R^CN is hydrogen.

[0027] In certain embodiments, the carrier is a protein, a lipid, a lipolized protein, a virus, a peptide comprising a T cell epitope, or a dendrimer of glycopeptides. In certain embodiments, the carrier is a toxin protein selected from the group consisting of diphtheria toxin cross-reacting material 197 (DT-CRM197), diphtheria toxoid, tetanus toxoid, and outer-membrane protein (OMP). In certain embodiments, the toxin protein is DT-CRM197.

[0029] In another aspect, the present invention provides a glycan conjugate mixture comprising at least two of the glycan conjugates as described herein. In certain embodiments, the average value of w in the glycan mixture is from about 1.0 to about 100.0. In certain embodiments, the average value of w in the glycan mixture is from about 1.0 to 10.0. In certain embodiments, the average value of w in the glycan mixture is about 5.7, 4.9, 2.9, 2.8, or 3.1. In certain embodiments, the average value of w in the glycan mixture is about 4.9, 2.9, 2.8, or 3.1.

[0030] In another aspect, the present invention provides methods of synthesizing the glycan conjugates as described herein.

[0031] In another aspect, the present invention provides immmunogenic compositions comprising a glycan conjugate or a glycan conjugate mixture as provided herein and a pharmaceutically acceptable excipient. In certain embodiments, the immmunogenic

compositions further comprise an adjuvant. In certain embodiments, the adjuvant is C34, 7DW8- 5, C17, C23, C30, a-galactoceramide, Aluminum salt, Squalene, MF59, or QS-21. In certain embodiments, the immmunogenic compositions described herein include an immmunogenically effective amount of an inventive glycan conjugate. In certain embodiments, the immmunogenic compositions described herein include an pharmaceutically effective amount of an inventive glycan conjugate. The invetive glycan conjugates described herein are useful for inducing an immune response against an infectious disease in a subject. In certain embodiments, the infectious disease is a bacterial infection. In certain embodiments, the infectious disease is caused by Gram-positive bacteria. In certain embodiments, the infectious disease is caused by Gram-negative bacteria. In certain embodiments, the infectious disease is caused by N.

meningitidis. In certain embodiments, the immmunogenic compositions provided herein induce IgG and IgM antibodies and provided an immunogenicity against the bacteria. In certaine embodiments, the immmunogenic compositions provided herein induce more IgG antibodies and IgM antibodies. In certaine embodiments, the immmunogenic compositions provided herein mainly induce IgGl and IgG3.

[0032] In another aspect, the present invention provides kits comprising the inventive glycan conjugates, or glycan conjugate mixture thereof, or immmunogenic compositions thereof. The kits of the invention may include a single dose or multiple doses of the inventive glycan conjugates, a glycan conjugate mixture thereof, or immmunogenic compositions thereof. The provided kits may be useful for treating or reducing the risk of infectious diseases such bacterial infections. The kits may also include packaging information describing the use or prescribing information for the subject or a health care professional. Such information may be required by a regulatory agency such as the U.S. Food and Drug Administration (FDA). The kit may also optionally include a device for administration of the compound or composition, for example, a syringe for parenteral administration.

[0033] In another aspect, the present invention provides methods for treating and/or reducing the risk for infectious diseases in a subject comprising administering to the subject

therapeutically effective amount of a glycan conjugate, or a glycan conjugate thereof, or an immunogenic composition as described herein.

[0034] In another aspect, the present invention provides methods for inhibiting bacterial growth in a subject comprising administering to a subject a therapeutically effective amount of a glycan conjugate, or a glycan conjugate mixture, or an immunogenic composition as described herein. In another aspect, the present invention provides methods for inhibiting bacterial infections comprising administering to a subject a therapeutically effective amount of a glycan conjugate, or a glycan conjugate mixture, or an immunogenic composition as described herein.

[0035] In certain embodiments, the bacterial infection is an infection with a Gram-positive bacterium. In certain embodiments, the bacterium is selected from the group consisting of Staphylococcus, Streptococcus, Micrococcus, Peptococcus, Peptostreptococcus, Enterococcus, Bacillus, Clostridium, Lactobacillus, Listeria, Erysipelothrix, Propionibacterium, Eubacterium, and Corynebacterium. In certain embodiments, the bacterial infection is an infection with a Gram-negative bacterium. In certain embodiments, the Gram-negative bacterium is selected from the group consisting of Escherchia coli, Caulobacter crescentus, Pseudomonas aeruginosa, Agrobacterium tumefaciens, Branhamella catarrhalis, Citrobacter diversus, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, Salmonella typhimurium, Neisseria meningitidis, Serratia marcescens, Shigella sonnei, Neisseria gonorrhoeae, Acinetobacter baumannii, Salmonella enteriditis, Fusobacterium nucleatum, Veillonella parvula, Bacteroides forsythus, Actinobacillus actinomycetemcomitans, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Helicobacter pylori, Francisella tularensis, Yersinia pestis, Morganella morganii, Edwardsiella tarda, Acinetobacter baumannii and Haemophilus influenzae. In certain embodiments, the bacterium is N. meningitides . In certain embodiments, the bacterium is N. meningitides serogroups W135.

[0036] In another aspect, the present invention provides methods for inhibiting bacterial cell growth comprising contacting the bacterial cell with a glycan conjugate, or a glycan conjugate mixture thereof, or an immunogenic composition thereof. In certain embodiments, the contacting step is performed in in vitro. In certain embodiments, the contacting step is performed by administering the glycan conjugate, the glycan conjugate mixture, or the immunogenic composition as described herein, to a subject in need thereof.

[0037] Also described herein are pharmaceutical compositions for use in eliciting immune responses against bacterial antigens and/or in treating bacterial infections, wherein the pharmaceutical compositions comprising a glycan-conjugate or a glycan conjugate mixture as described herein and a pharmaceutically acceptable expicient. In certain embodiments, the provided pharmaceutical compositions further comprise an adjuvant. In certain embodiments, provided herein is a pharmaceutical compositions for use in manufacturing medicant for use in bacterial immunotherapy.

[0038] In another aspect, the present invention provides methods of preparing the inventive glycan conjugates, the glycan conjugate mixtures thereof, or immunogenic compositions thereof.

[0039] In certain embodiments, the invention provides a method of preparing a glycan conjugate as described herein, comprising coupling a compound of Formula (C-l)

(C-l)

or a salt thereof,

with a compound of the Formula (C-2)

(C-2) wherein L^2C is a crosslinking reagent capable of crosslinking an amino group and L¹-!!.

[0040] In certain embodiments, L is a crosslinking reagent capable of crosslinking an group and -SH. In cert rmulae:

or a salt thereof,

wherein each instance of R^P1 and R^P2are each independently hydrogen, halogen, or optionally substituted Ci_6 alkyl; each instance of R^Na is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group; each instance of R^2Ca is a leaving group selected from selected from -Br, -CI, -I, -OS(=0)₂R^2CO, or -OS(=0)R^2CO, wherein R^2CO is selected from the group consisting of optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl; and each of t and i is independently an integer of 1 to 8, inclusive.

[0041] In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 1 to about 100. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 1 to about 80. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 1 to about 60. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 1 to about 40. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 1 to about 20. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 1 to about 10. In certain embodiments, the coupling is carried out in the presence of phosphate buffered saline (PBS).

[0042] In certain embodiments, the method further comprises glycosylating a compound of Formula (C-3)

with a compound of Formula (C-4)

(C-5)

wherein v is an integer of 1 to 99, inclusive; u is 0, 1, 2, 3, 4, or 5; each occurrence of ^E is independently halogen, -CN, -NO₂, -N3, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted phenyl, optionally substituted heterocyclyl, optionally substituted heteroaryl, -OR^CE, -N(R^CE)₂, - SR^CE, -C(=0)R^CE -C(=0)OR^CE, or -C(=0)N(R^CE)₂, wherein each R^CE is independently selected from the group consisting of hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl; or an oxygen protecting group when attached to an oxygen, or a nitrogen protecting group when attached to nitrogen, or a sulfur protecting group when attached to sulfur; and R^N3 is independently hydrogen, optionally substituted alkyl, or a nitrogen protecting group.

[0043] In certain embodiments, the method further comprises reacting the compound of Formula (C-5) in the presence of a base to give a compound of Formula (C-6)

(C-6)

[0044] In certain embodiments, the base is NaOCH₃.

[0045] In certain embodiments, the method further comprises reacting the compound of Formula (C-6) with a r -7)

(C-7)

[0046] In certain embodiments, the method further comprises: (a) activating a compound of Formula (C-7) to give a compound of Formula (C-l); and (b) activating a carrier to give a compound of Formula (C-2).

[0047] The details of certain embodiments of the invention are set forth herein. Other features, objects, and advantages of the invention will be apparent from the Detailed Description, the Figures, the Examples, and the Claims.

BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 shows structure of N. meningitidis serogroup W135 capsular oligosaccharide. The oligosaccahride is composed of a repeating unit of^→6)- a -D-Galp-(1^→4)- a -D- Neup5Ac(90Ac)-(2→.

[0049] Figure 2 shows immunogenicity comparison by microarray. Antibodies elicited by DT- 4 or longer-oligosaccharide conjugates had higher binding affinity with longer oligosaccharide, and DT-8 recruited most abundant quantity of antibody.

[0050] Figure 3 shows exemplary synthesis of a compound of Formula (F-l).

[0051] Figure 4 shows synthesis of disaccharide to decasaccharide. [0052] Figure 5 shows oligosaccharide conjugated to the carrier.

[0053] Figure 6 shows immunogenicity comparison by microarray. (a) Antibody elicited by DT-2 bound to disaccharide compound 13 and other similar structure disaccharides on the chip, (b) Antibody elicited by DT-4 bound to compounds 16, 19, 22, and 25, and antibodies elicited by DT-6, DT-8, and DT-10 also performed the same pattern on the microarray. The antibody had high specificity against N. meningitidis serogroup W135 capsular oligosaccharide.

[0054] Figure 7 shows the immunogenicity of various conjugates with alum adjuvant. With alum as adjuvant, the conjugates also performed the same pattern as with C34 adjuvant. DT-2 only recognized disaccharide compound, and DT-4 to DT-10 all recognized all oligosaccharide

[0055] Figure 8 shows IgG subclasses expression. As a TD-antigen, IgGl was the major IgG subclasses group in the serum. In particular, there was a high level of IgG3 in the serum, which is the anti-carbohydrate response.

[0056] Figure 9 shows the exemplified crosslinking reactions of an glycan conjugate with a carrier.

[0057] Figure 10 shows an exemplified hybrid glycan conjugate.

DETAILED DESCRIPTION OF THE INVENTION

Chemical definitions

[0058] Definitions of specific functional groups and chemical terms are described in more detail below. The chemical elements are identified in accordance with the Periodic Table of the Elements, CAS version, Handbook of Chemistry and Physics, 75^th Ed., inside cover, and specific functional groups are generally defined as described therein. Additionally, general principles of organic chemistry, as well as specific functional moieties and reactivity, are described in Thomas Sorrell, Organic Chemistry, University Science Books, Sausalito, 1999; Smith and March, March 's Advanced Organic Chemistry, 5^th Edition, John Wiley & Sons, Inc., New York, 2001; Larock, Comprehensive Organic Transformations, VCH Publishers, Inc., New York, 1989; and Carruthers, Some Modern Methods of Organic Synthesis, 3^rd Edition, Cambridge University Press, Cambridge, 1987.

[0059] Compounds described herein can comprise one or more asymmetric centers, and thus can exist in various isomeric forms, e.g., enantiomers and/or diastereomers. For example, the compounds described herein can be in the form of an individual enantiomer, diastereomer or geometric isomer, or can be in the form of a mixture of stereoisomers, including racemic mixtures and mixtures enriched in one or more stereoisomer. Isomers can be isolated from mixtures by methods known to those skilled in the art, including chiral high pressure liquid chromatography (HPLC) and the formation and crystallization of chiral salts; or preferred isomers can be prepared by asymmetric syntheses. See, for example, Jacques et al., Enantiomers, Racemates and Resolutions (Wiley Interscience, New York, 1981); Wilen et al., Tetrahedron 33 :2725 (1977); Eliel, Stereochemistry of Carbon Compounds (McGraw-Hill, NY, 1962); and Wilen, Tables of Resolving Agents and Optical Resolutions p. 268 (E.L. Eliel, Ed., Univ. of Notre Dame Press, Notre Dame, IN 1972). The invention additionally encompasses compounds described herein as individual isomers substantially free of other isomers, and alternatively, as mixtures of various isomers.

[0060] When a range of values is listed, it is intended to encompass each value and sub-range within the range. For example "Ci_6" is intended to encompass Ci, C2, C3, C₄, C5, Ce, Ci_6, G_s,

Ci_4, Ci_3, Ci_2, C2-6, C2-5, C2-4, C2-3, C3_6, C3_s, C3_4, C4_6, C₄_5, a d C5_6.

[0061] "Alkyl" refers to a radical of a straight-chain or branched saturated hydrocarbon group having from 1 to 20 carbon atoms ("Ci_₂o alkyl"). In some embodiments, an alkyl group has 1 to 10 carbon atoms ("Ci_io alkyl"). In some embodiments, an alkyl group has 1 to 9 carbon atoms ("Ci_9 alkyl"). In some embodiments, an alkyl group has 1 to 8 carbon atoms ("Ci_8 alkyl"). In some embodiments, an alkyl group has 1 to 7 carbon atoms ("Ci_₇ alkyl"). In some embodiments, an alkyl group has 1 to 6 carbon atoms ("Ci_₆ alkyl"). In some embodiments, an alkyl group has 1 to 5 carbon atoms ("Ci_5 alkyl"). In some embodiments, an alkyl group has 1 to 4 carbon atoms ("Ci_4 alkyl"). In some embodiments, an alkyl group has 1 to 3 carbon atoms ("Ci_3 alkyl"). In some embodiments, an alkyl group has 1 to 2 carbon atoms ("Ci_₂ alkyl"). In some embodiments, an alkyl group has 1 carbon atom ("Ci alkyl"). In some embodiments, an alkyl group has 2 to 6 carbon atoms ("C₂-6 alkyl"). Examples of Ci_6 alkyl groups include methyl (Ci), ethyl (C₂), «-propyl (C₃), wo-propyl (C₃), H-butyl (C₄), tert-but l (C₄), sec-butyl (C₄), z^'so-butyl (C₄), «-pentyl (C₅), 3-pentanyl (C₅), amyl (C₅), neopentyl (C₅), 3-methyl-2-butanyl (C₅), tertiary amyl (C5), and κ-hexyl (Ce). Additional examples of alkyl groups include «-heptyl (C₇), K-octyl (Cg) and the like. Unless otherwise specified, each instance of an alkyl group is independently optionally substituted, i.e., unsubstituted (an "unsubstituted alkyl") or substituted (a "substituted alkyl") with one or more substituents. In certain embodiments, the alkyl group is unsubstituted Cuo alkyl {e.g., -CH₃). In certain embodiments, the alkyl group is substituted G_ ₁0 alkyl.

[0062] "Alkenyl" refers to a radical of a straight-chain or branched hydrocarbon group having from 2 to 20 carbon atoms, one or more carbon-carbon double bonds, and no triple bonds ("C₂- ₂₀ alkenyl"). In some embodiments, an alkenyl group has 2 to 10 carbon atoms ("C₂_io alkenyl"). In some embodiments, an alkenyl group has 2 to 9 carbon atoms ("C₂-9 alkenyl"). In some embodiments, an alkenyl group has 2 to 8 carbon atoms ("C₂_8 alkenyl"). In some embodiments, an alkenyl group has 2 to 7 carbon atoms ("C₂-7 alkenyl"). In some embodiments, an alkenyl group has 2 to 6 carbon atoms ("C2-6 alkenyl"). In some embodiments, an alkenyl group has 2 to 5 carbon atoms ("C2-5 alkenyl"). In some embodiments, an alkenyl group has 2 to 4 carbon atoms ("C2-4 alkenyl"). In some embodiments, an alkenyl group has 2 to 3 carbon atoms ("C2-3 alkenyl"). In some embodiments, an alkenyl group has 2 carbon atoms ("C2 alkenyl"). The one or more carbon-carbon double bonds can be internal (such as in 2-butenyl) or terminal (such as in 1-butenyl). Examples of

alkenyl groups include ethenyl (C2), 1-propenyl (C₃), 2- propenyl (C₃), 1-butenyl (C₄), 2-butenyl (C₄), butadienyl (C₄), and the like. Examples of 2-6 alkenyl groups include the aforementioned C2-4 alkenyl groups as well as pentenyl (C5), pentadienyl (C5), hexenyl (Ce), and the like. Additional examples of alkenyl include heptenyl (C₇), octenyl (Cg), octatrienyl (Cg), and the like. Unless otherwise specified, each instance of an alkenyl group is independently optionally substituted, i.e. , unsubstituted (an "unsubstituted alkenyl") or substituted (a "substituted alkenyl") with one or more substituents. In certain embodiments, the alkenyl group is unsubstituted C2-10 alkenyl. In certain embodiments, the alkenyl group is substituted C2-10 alkenyl.

[0063] "Alkynyl" refers to a radical of a straight-chain or branched hydrocarbon group having from 2 to 20 carbon atoms, one or more carbon-carbon triple bonds, and optionally one or more double bonds ("C2-20 alkynyl"). In some embodiments, an alkynyl group has 2 to 10 carbon atoms ("C2-10 alkynyl"). In some embodiments, an alkynyl group has 2 to 9 carbon atoms ("C2-9 alkynyl"). In some embodiments, an alkynyl group has 2 to 8 carbon atoms ("C2-8 alkynyl"). In some embodiments, an alkynyl group has 2 to 7 carbon atoms ("C2-7 alkynyl"). In some embodiments, an alkynyl group has 2 to 6 carbon atoms ("C2-6 alkynyl"). In some embodiments, an alkynyl group has 2 to 5 carbon atoms ("C2-5 alkynyl"). In some embodiments, an alkynyl group has 2 to 4 carbon atoms ("C₂-₄ alkynyl"). In some embodiments, an alkynyl group has 2 to 3 carbon atoms ("C2-3 alkynyl"). In some embodiments, an alkynyl group has 2 carbon atoms ("C2 alkynyl"). The one or more carbon-carbon triple bonds can be internal (such as in 2- butynyl) or terminal (such as in 1-butynyl). Examples of C2-4 alkynyl groups include, without limitation, ethynyl (C2), 1-propynyl (C3), 2-propynyl (C3), 1-butynyl (C4), 2-butynyl (C₄), and the like. Examples of C2-6 alkenyl groups include the aforementioned C2-4 alkynyl groups as well as pentynyl (C₅), hexynyl (Ce), and the like. Additional examples of alkynyl include heptynyl (C₇), octynyl (Cg), and the like. Unless otherwise specified, each instance of an alkynyl group is independently optionally substituted, i.e. , unsubstituted (an "unsubstituted alkynyl") or substituted (a "substituted alkynyl") with one or more substituents. In certain embodiments, the alkynyl group is unsubstituted C2-10 alkynyl. In certain embodiments, the alkynyl group is substituted C₂_io alkynyl. [0064] "Carbocyclyl" or "carbocyclic" refers to a radical of a non-aromatic cyclic hydrocarbon group having from 3 to 10 ring carbon atoms ("C3_io carbocyclyl") and zero heteroatoms in the non-aromatic ring system. In some embodiments, a carbocyclyl group has 3 to 8 ring carbon atoms ("C3_8 carbocyclyl"). In some embodiments, a carbocyclyl group has 3 to 6 ring carbon atoms ("C₃__s carbocyclyl"). In some embodiments, a carbocyclyl group has 3 to 6 ring carbon atoms ("C3_6 carbocyclyl"). In some embodiments, a carbocyclyl group has 5 to 10 ring carbon atoms ("Cs-io carbocyclyl"). Exemplary C -6 carbocyclyl groups include, without limitation, cyclopropyl (C3), cyclopropenyl (C3), cyclobutyl (C₄), cyclobutenyl (C₄), cyclopentyl (Cs), cyclopentenyl (Cs), cyclohexyl (Ce), cyclohexenyl (Ce), cyclohexadienyl (Ce), and the like. Exemplary C _g carbocyclyl groups include, without limitation, the aforementioned C _6 carbocyclyl groups as well as cycloheptyl (C₇), cycloheptenyl (C₇), cycloheptadienyl (C₇), cycloheptatrienyl (C7), cyclooctyl (Cs), cyclooctenyl (Cs), 17ydroxy[2.2.1]heptanyl (C7), 17ydroxy[2.2.2]octanyl (Cg), and the like. Exemplary C3_io carbocyclyl groups include, without limitation, the aforementioned C _s carbocyclyl groups as well as cyclononyl (C₉), cyclononenyl (C9), cyclodecyl (C10), cyclodecenyl (C10), octahydro-lH-indenyl (Cg), decahydronaphthalenyl (Cio), spiro[4.5]decanyl (Ci₀), and the like. As the foregoing examples illustrate, in certain embodiments, the carbocyclyl group is either monocyclic ("monocyclic carbocyclyl") or contain a fused, bridged or spiro ring system such as a bicyclic system ("bicyclic carbocyclyl") and can be saturated or can be partially unsaturated. "Carbocyclyl" also includes ring systems wherein the carbocyclic ring, as defined above, is fused to one or more aryl or heteroaryl groups wherein the point of attachment is on the carbocyclic ring, and in such instances, the number of carbons continue to designate the number of carbons in the carbocyclic ring system. Unless otherwise specified, each instance of a carbocyclyl group is independently optionally substituted, i. e. , unsubstituted (an "unsubstituted carbocyclyl") or substituted (a "substituted carbocyclyl") with one or more substituents. In certain embodiments, the carbocyclyl group is unsubstituted C3_io carbocyclyl. In certain embodiments, the carbocyclyl group is a substituted C3_io carbocyclyl.

[0065] In some embodiments, "carbocyclyl" is a monocyclic, saturated carbocyclyl group having from 3 to 10 ring carbon atoms ("C3_io cycloalkyl"). In some embodiments, a cycloalkyl group has 3 to 8 ring carbon atoms ("C3_g cycloalkyl"). In some embodiments, a cycloalkyl group has 3 to 6 ring carbon atoms ("C3_6 cycloalkyl"). In some embodiments, a cycloalkyl group has 5 to 6 ring carbon atoms ("Cs_6 cycloalkyl"). In some embodiments, a cycloalkyl group has 5 to 10 ring carbon atoms ("Cs_io cycloalkyl"). Examples of Cs_6 cycloalkyl groups include cyclopentyl (C₅) and cyclohexyl (C₅). Examples of C3_6 cycloalkyl groups include the aforementioned C₅_₆ cycloalkyl groups as well as cyclopropyl (C₃) and cyclobutyl (C₄).

Examples of C3_g cycloalkyl groups include the aforementioned C3_e cycloalkyl groups as well as cycloheptyl (C₇) and cyclooctyl (Cg). Unless otherwise specified, each instance of a cycloalkyl group is independently unsubstituted (an "unsubstituted cycloalkyl") or substituted (a "substituted cycloalkyl") with one or more substituents. In certain embodiments, the cycloalkyl group is unsubstituted C3_io cycloalkyl. In certain embodiments, the cycloalkyl group is substituted C₃_i₀ cycloalkyl.

[0066] "Heterocyclyl" or "heterocyclic" refers to a radical of a 3- to 10-membered non- aromatic ring system having ring carbon atoms and 1 to 4 ring heteroatoms, wherein each heteroatom is independently selected from nitrogen, oxygen, sulfur, boron, phosphorus, and silicon ("3-10 membered heterocyclyl"). In certain embodiments, the heteroatom is

independently selected from nitrogen, sulfur, and oxygen. In heterocyclyl groups that contain one or more nitrogen atoms, the point of attachment can be a carbon or nitrogen atom, as valency permits. A heterocyclyl group can either be monocyclic ("monocyclic heterocyclyl") or a fused, bridged or spiro ring system such as a bicyclic system ("bicyclic heterocyclyl"), and can be saturated or partially unsaturated. Heterocyclyl bicyclic ring systems can include one or more heteroatoms in one or both rings. "Heterocyclyl" also includes ring systems wherein the heterocyclic ring, as defined above, is fused with one or more carbocyclyl groups wherein the point of attachment is either on the carbocyclyl or heterocyclic ring, or ring systems wherein the heterocyclic ring, as defined above, is fused with one or more aryl or heteroaryl groups, wherein the point of attachment is on the heterocyclic ring, and in such instances, the number of ring members continue to designate the number of ring members in the heterocyclic ring system. Unless otherwise specified, each instance of heterocyclyl is independently optionally substituted, i.e., unsubstituted (an "unsubstituted heterocyclyl") or substituted (a "substituted heterocyclyl") with one or more substituents. In certain embodiments, the heterocyclyl group is unsubstituted 3-10 membered heterocyclyl. In certain embodiments, the heterocyclyl group is substituted 3- 10 membered heterocyclyl.

[0067] In some embodiments, a heterocyclyl group is a 5-10 membered non-aromatic ring system having ring carbon atoms and 1-4 ring heteroatoms, wherein each heteroatom is independently selected from nitrogen, oxygen, sulfur, boron, phosphorus, and silicon ("5-10 membered heterocyclyl"). In some embodiments, a heterocyclyl group is a 5-8 membered non- aromatic ring system having ring carbon atoms and 1-4 ring heteroatoms, wherein each heteroatom is independently selected from nitrogen, oxygen, and sulfur ("5-8 membered heterocyclyl"). In some embodiments, a heterocyclyl group is a 5-6 membered non-aromatic ring system having ring carbon atoms and 1-4 ring heteroatoms, wherein each heteroatom is independently selected from nitrogen, oxygen, and sulfur ("5-6 membered heterocyclyl"). In some embodiments, the 5-6 membered heterocyclyl has 1-3 ring heteroatoms selected from nitrogen, oxygen, and sulfur. In some embodiments, the 5-6 membered heterocyclyl has 1-2 ring heteroatoms selected from nitrogen, oxygen, and sulfur. In some embodiments, the 5-6 membered heterocyclyl has one ring heteroatom selected from nitrogen, oxygen, and sulfur.

[0068] Exemplary 3-membered heterocyclyl groups containing one heteroatom include, without limitation, azirdinyl, oxiranyl, and thiorenyl. Exemplary 4-membered heterocyclyl groups containing one heteroatom include, without limitation, azetidinyl, oxetanyl, and thietanyl. Exemplary 5-membered heterocyclyl groups containing one heteroatom include, without limitation, tetrahydrofuranyl, dihydrofuranyl, tetrahydrothiophenyl, dihydrothiophenyl, pyrrolidinyl, dihydropyrrolyl, and pyrrolyl-2,5-dione. Exemplary 5-membered heterocyclyl groups containing two heteroatoms include, without limitation, dioxolanyl, oxasulfuranyl, disulfuranyl, and oxazolidin-2-one. Exemplary 5-membered heterocyclyl groups containing three heteroatoms include, without limitation, triazolinyl, oxadiazolinyl, and thiadiazolinyl. Exemplary 6-membered heterocyclyl groups containing one heteroatom include, without limitation, piperidinyl, tetrahydropyranyl, dihydropyridinyl, and thianyl. Exemplary 6- membered heterocyclyl groups containing two heteroatoms include, without limitation, piperazinyl, morpholinyl, dithianyl, and dioxanyl. Exemplary 6-membered heterocyclyl groups containing two heteroatoms include, without limitation, triazinanyl. Exemplary 7-membered heterocyclyl groups containing one heteroatom include, without limitation, azepanyl, oxepanyl, and thiepanyl. Exemplary 8-membered heterocyclyl groups containing one heteroatom include, without limitation, azocanyl, oxecanyl, and thiocanyl. Exemplary 5-membered heterocyclyl groups fused to a Ce aryl ring (also referred to herein as a 5,6-bicyclic heterocyclic ring) include, without limitation, indolinyl, isoindolinyl, dihydrobenzofuranyl, dihydrobenzothienyl, benzoxazolinonyl, and the like. Exemplary 6-membered heterocyclyl groups fused to an aryl ring (also referred to herein as a 6,6-bicyclic heterocyclic ring) include, without limitation, tetrahydroquinolinyl, tetrahydroisoquinolinyl, and the like.

[0069] "Aryl" refers to a radical of a monocyclic or polycyclic (e.g., bicyclic or tricyclic) 4n+2 aromatic ring system (e.g., having 6, 10, or 14 π electrons shared in a cyclic array) having 6-14 ring carbon atoms and zero heteroatoms in the aromatic ring system ("C6-1 aryl"). In some embodiments, an aryl group has six ring carbon atoms ("Ce aryl"; e.g., phenyl). In some embodiments, an aryl group has ten ring carbon atoms ("C₁₀ aryl"; e.g., naphthyl such as 1- naphthyl and 2-naphthyl). In some embodiments, an aryl group has fourteen ring carbon atoms ("Ci₄ aryl"; e.g., anthracyl). "Aryl" also includes ring systems wherein the aryl ring, as defined above, is fused with one or more carbocyclyl or heterocyclyl groups wherein the radical or point of attachment is on the aryl ring, and in such instances, the number of carbon atoms continue to designate the number of carbon atoms in the aryl ring system. Unless otherwise specified, each instance of an aryl group is independently optionally substituted, i. e. , unsubstituted (an

"unsubstituted aryl") or substituted (a "substituted aryl") with one or more substituents. In certain embodiments, the aryl group is unsubstituted Ce- aryl. In certain embodiments, the aryl group is substituted Ce-i aryl.

[0070] "Arylalkyl" is a subset of alkyl and aryl, as defined herein, and refers to an optionally substituted alkyl group substituted by an optionally substituted aryl group. In certain embodiments, the aralkyl is optionally substituted benzyl. In certain embodiments, the aralkyl is benzyl. In certain embodiments, the aralkyl is optionally substituted phenethyl. In certain embodiments, the aralkyl is phenethyl.

[0071] "Heteroaryl" refers to a radical of a 5-10 membered monocyclic or bicyclic 4n+2 aromatic ring system (e.g., having 6 or 10 π electrons shared in a cyclic array) having ring carbon atoms and 1-4 ring heteroatoms provided in the aromatic ring system, wherein each heteroatom is independently selected from nitrogen, oxygen, and sulfur ("5-10 membered heteroaryl"). In heteroaryl groups that contain one or more nitrogen atoms, the point of attachment can be a carbon or nitrogen atom, as valency permits. Heteroaryl bicyclic ring systems can include one or more heteroatoms in one or both rings. "Heteroaryl" includes ring systems wherein the heteroaryl ring, as defined above, is fused with one or more carbocyclyl or heterocyclyl groups wherein the point of attachment is on the heteroaryl ring, and in such instances, the number of ring members continue to designate the number of ring members in the heteroaryl ring system. "Heteroaryl" also includes ring systems wherein the heteroaryl ring, as defined above, is fused with one or more aryl groups wherein the point of attachment is either on the aryl or heteroaryl ring, and in such instances, the number of ring members designates the number of ring members in the fused (aryl/heteroaryl) ring system. Bicyclic heteroaryl groups wherein one ring does not contain a heteroatom (e.g., indolyl, quinolinyl, carbazolyl, and the like) the point of attachment can be on either ring, i.e., either the ring bearing a heteroatom (e.g., 2-indolyl) or the ring that does not contain a heteroatom (e.g., 5-indolyl).

[0072] In some embodiments, a heteroaryl group is a 5-10 membered aromatic ring system having ring carbon atoms and 1-4 ring heteroatoms provided in the aromatic ring system, wherein each heteroatom is independently selected from nitrogen, oxygen, and sulfur ("5-10 membered heteroaryl"). In some embodiments, a heteroaryl group is a 5-8 membered aromatic ring system having ring carbon atoms and 1-4 ring heteroatoms provided in the aromatic ring system, wherein each heteroatom is independently selected from nitrogen, oxygen, and sulfur ("5-8 membered heteroaryl"). In some embodiments, a heteroaryl group is a 5-6 membered aromatic ring system having ring carbon atoms and 1-4 ring heteroatoms provided in the aromatic ring system, wherein each heteroatom is independently selected from nitrogen, oxygen, and sulfur ("5-6 membered heteroaryl"). In some embodiments, the 5-6 membered heteroaryl has 1-3 ring heteroatoms selected from nitrogen, oxygen, and sulfur. In some embodiments, the 5-6 membered heteroaryl has 1-2 ring heteroatoms selected from nitrogen, oxygen, and sulfur. In some embodiments, the 5-6 membered heteroaryl has 1 ring heteroatom selected from nitrogen, oxygen, and sulfur. Unless otherwise specified, each instance of a heteroaryl group is independently optionally substituted, i.e., unsubstituted (an "unsubstituted heteroaryl") or substituted (a "substituted heteroaryl") with one or more substituents. In certain embodiments, the heteroaryl group is unsubstituted 5-14 membered heteroaryl. In certain embodiments, the heteroaryl group is substituted 5-14 membered heteroaryl.

[0073] Exemplary 5-membered heteroaryl groups containing one heteroatom include, without limitation, pyrrolyl, furanyl and thiophenyl. Exemplary 5-membered heteroaryl groups containing two heteroatoms include, without limitation, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, and isothiazolyl. Exemplary 5-membered heteroaryl groups containing three heteroatoms include, without limitation, triazolyl, oxadiazolyl, and thiadiazolyl.

Exemplary 5-membered heteroaryl groups containing four heteroatoms include, without limitation, tetrazolyl. Exemplary 6-membered heteroaryl groups containing one heteroatom include, without limitation, pyridinyl. Exemplary 6-membered heteroaryl groups containing two heteroatoms include, without limitation, pyridazinyl, pyrimidinyl, and pyrazinyl. Exemplary 6- membered heteroaryl groups containing three or four heteroatoms include, without limitation, triazinyl and tetrazinyl, respectively. Exemplary 7-membered heteroaryl groups containing one heteroatom include, without limitation, azepinyl, oxepinyl, and thiepinyl. Exemplary 5,6- bicyclic heteroaryl groups include, without limitation, indolyl, isoindolyl, indazolyl, benzotriazolyl, benzothiophenyl, isobenzothiophenyl, benzofuranyl, benzoisofuranyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzoxadiazolyl, benzthiazolyl, benzisothiazolyl, benzthiadiazolyl, indolizinyl, and purinyl. Exemplary 6,6-bicyclic heteroaryl groups include, without limitation, naphthyridinyl, pteridinyl, quinolinyl, isoquinolinyl, cinnolinyl, quinoxalinyl, phthalazinyl, and quinazolinyl.

[0074] "Heteroaralkyl" is a subset of alkyl and heteroaryl, as defined herein, and refers to an optionally substituted alkyl group substituted by an optionally substituted heteroaryl group.

[0075] "Partially unsaturated" refers to a group that includes at least one double or triple bond. A "partially unsaturated" ring system is further intended to encompass rings having multiple sites of unsaturation, but is not intended to include aromatic groups (e.g., aryl or heteroaryl groups) as herein defined. Likewise, "saturated" refers to a group that does not contain a double or triple bond, i.e., it contains all single bonds. [0076] Alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl groups, as defined herein, which are divalent bridging groups are further referred to using the suffix -ene, e.g., alkylene, alkenylene, alkynylene, carbocyclylene, heterocyclylene, arylene, and heteroarylene.

[0077] As used herein, the term "optionally substituted" refers to a substituted or unsubstituted moiety.

[0078] Alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl groups, as defined herein, are optionally substituted {e.g., "substituted" or "unsubstituted" alkyl,

"substituted" or "unsubstituted" alkenyl, "substituted" or "unsubstituted" alkynyl, "substituted" or "unsubstituted" carbocyclyl, "substituted" or "unsubstituted" heterocyclyl, "substituted" or "unsubstituted" aryl or "substituted" or "unsubstituted" heteroaryl group). In general, the term "substituted", whether preceded by the term "optionally" or not, means that at least one hydrogen present on a group {e.g., a carbon or nitrogen atom) is replaced with a permissible substituent, e.g., a substituent which upon substitution results in a stable compound, e.g., a compound which does not spontaneously undergo transformation such as by rearrangement, cyclization, elimination, or other reaction. Unless otherwise indicated, a "substituted" group has a substituent at one or more substitutable positions of the group, and when more than one position in any given structure is substituted, the substituent is either the same or different at each position. The term "substituted" is contemplated to include substitution with all permissible substituents of organic compounds, any of the substituents described herein that results in the formation of a stable compound. The present invention contemplates any and all such combinations in order to arrive at a stable compound. For purposes of this invention, heteroatoms such as nitrogen may have hydrogen substituents and/or any suitable substituent as described herein which satisfy the valencies of the heteroatoms and results in the formation of a stable moiety.

[0079] Exemplary carbon atom substituents include, but are not limited to, halogen, -CN, - N0₂, -N₃, -S0₂H, -SO3H, -OH, -OIT, -ON(R^bb)₂, -N(R^bb)₂, -N(R^bb)₃ ⁺X ^" -N(OR^cc)R^bb, -SH, -SR^aa, -SSR^CC, -C(=0)R^aa, -C0₂H, -CHO, -C(OR^cc)₂, -C0₂R^aa, -OC(=0)R^aa, -OC0₂R^aa, - C(=0)N(R^bb)₂, -OC(=0)N(R^bb)₂, - R^bbC(=0)R^aa, -NR^bbC0₂R^aa, -NR^bbC(=0)N(R^bb)₂, - C(=NR^bb)R^aa, -C(=NR^bb)OR^aa, -OC(=NR^bb)R^aa, -OC(=NR^bb)OR^aa, -C(=NR^bb)N(R^bb)₂, - OC(=NR^bb)N(R^bb)₂, -NR^bbC(=NR^bb)N(R^bb)₂, -C(=0)NR^bbS0₂R^aa, -NR^bbS0₂ "^a, -S0₂N(R^bb)₂, -SO₂R", -S0₂OR^aa, -OS0₂R^aa, -S(=0)R^aa, -OS(=0)R^aa, -Si(R^aa)₃, -Osi^s -C(=S)N(R^bb)₂, - C(=0)SR^aa, -C(=S)SR^aa, -SC(=S)SR^aa, -SC(=0)SR^aa, -OC(=0)SR^aa, -SC(=0)OR^aa, - SC(=0)R^aa, -P(=0)₂R^aa, -OP(=0)₂R^aa, -P(=0)(R^aa)₂, -OP(=0)(R^aa)₂, -OP(=0)(OR^cc)₂, - P(=0)₂N(R^bb)₂, -OP(=0)₂N(R^bb)₂, -P(=0)(NR^bb)₂, -OP(=0)(NR^bb)₂, -NR^bbP(=0)(OR^cc)₂, - NR^bbP(=0)(NR^bb)₂, -P(R^CC)₂, -P(R^CC)₃, -OP(R^cc)₂, -OP(R^cc)₃, -B(R^aa)₂, -B(OR^cc)₂, -BR^aa(OR^cc), Ci_io alkyl, CHO perhaloalkyl, C₂_io alkenyl, C₂-₁0 alkynyl, C₃_io carbocyclyl, 3-14 membered heterocyclyl, Ce-14 aryl, and 5-14 membered heteroaryl, wherein each alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1 , 2, 3, 4, or 5 R^dd groups; or two hydrogens on a carbon atom are replaced with the group =0, =S, = (R^bb)₂, =NNR^bbC(=0)R^aa, =NNR^bbC(=0)OR^aa, =N R^bbS(=0)₂R^aa, =NR^bb, or =NOR^cc; each instance of R^aa is, independently, selected from Ci_io alkyl, Cno perhaloalkyl, C₂-10 alkenyl, C₂-₁₀ alkynyl, C3-10 carbocyclyl, 3-14 membered heterocyclyl, -u aryl, and 5-14 membered heteroaryl, or two R^aa groups are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1 , 2, 3, 4, or 5 R^dd groups; each instance of R^bb is,

independently, selected from hydrogen, -OH, -OR^aa, -N(R^CC)₂, -CN, -C(=0)R^aa, - C(=0)N(R^cc)₂, -COzR³³, -S0₂R^aa, -C(=NR^cc)OR^aa, -C(=NR^CC)N(R^CC)₂, -S0₂N(R^cc)₂, -SOzR", - S0₂OR^cc, -SOR", -C(=S)N(R^CC)₂, -C(=0)SR^cc, -C(=S)SR^∞, -P(=0)₂R^aa, -P(=0)(R^aa)₂, - P(=0)₂N(R^cc)₂, -P(=0)( R^cc)₂, CHO alkyl, Cno perhaloalkyl, C₂-w alkenyl, C2-10 alkynyl, C3-10 carbocyclyl, 3-14 membered heterocyclyl, C₆_H aryl, and 5-14 membered heteroaryl, or two R^bb groups are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1 , 2, 3, 4, or 5 R^dd groups; each instance of R^cc is,

independently, selected from hydrogen, CHO alkyl, Ci-10 perhaloalkyl, C₂_io alkenyl, C₂_io alkynyl, C3-₁0 carbocyclyl, 3-14 membered heterocyclyl, Ce-14 aryl, and 5-14 membered heteroaryl, or two R^cc groups are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1 , 2, 3 , 4, or 5 R^dd groups; each instance of R^dd is, independently, selected from halogen, -CN, -N0₂, -N₃, -S0₂H, -SO₃H, -OH, -OR^ee, - ON(R^ff)₂, -N(R^ff)₂, -N(R^ff)₃ ⁺X^~, -NCOR^R^ -SH, -SR^ee, -SSR^ee, -C(=0)R^ee, -C0₂H, - C0₂R^ee, -OC(=0)R^ee, -OC0₂R^ee, -C(=0)N(R^ff)₂, -OC(=0)N(R^ff)₂, -NR^ffC(=0)R^ee, - NR^FFC0₂R^ee, -NR^ffC(=0)N(R^ff)₂, -C(=NR^ff)OR^ee, -OC(=NR^ff)R^ee, -OC(=NR^ff)OR^ee, - C(=NR^ff)N(R^ff)₂, -OC(=NR^ff)N(R^ff)₂, -NR^ffC(=NR^ff)N(R^ff)₂,-NR^FFS0₂R^ee, -S0₂N(R^ff)₂, - S0₂R^ee, -S0₂OR^ee, -OS0₂R^ee, -S(=0)R^ee, -Si(R^ee)₃, -Osi(R^ee)₃, -C(=S)N(R^ff)₂, -C(=0)SR^ee, - C(=S)SR^ee, -SC(=S)SR^ee, -P(=0)₂R^ee, -P(=0)(R^ee)₂, -OP(=0)(R^ee)₂, -OP(=0)(OR^ee)₂, d_₆ alkyl, Ci_e perhaloalkyl, C₂_6 alkenyl, C₂_e alkynyl, C₃_io carbocyclyl, 3-10 membered heterocyclyl, Ce-io aryl, 5-10 membered heteroaryl, wherein each alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1 , 2, 3, 4, or 5 R^sg groups, or two R^dd substituents can be joined to form =0 or =S; each instance of R^ee is, independently, selected from Ci_6 alkyl, Ci_6 perhaloalkyl, C₂_6 alkenyl, C₂_6 alkynyl, C3_io carbocyclyl, Ce-ιο aryl, 3-10 membered heterocyclyl, and 3-10 membered heteroaryl, wherein each alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1 , 2, 3, 4, or 5 R⁸⁸ groups; each instance of R^ff is, independently, selected from hydrogen, Ci_e alkyl, d_6 perhaloalkyl, C₂-6 alkenyl, C₂_₆ alkynyl, C₃_i₀ carbocyclyl, 3-10 membered heterocyclyl, Ce-io aryl and 5-10 membered heteroaryl, or two R^ff groups are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, I , 2, 3, 4, or 5 R⁸⁸ groups; and each instance of R^gg is, independently, halogen, -CN, -NO2, - N₃, -SO2H, -SO3H, -OH, -OCi_6 alkyl, -ON(Ci_₆ alkyl)₂, -N(Ci_6 alkyl)₂, -N(Ci_₆ alkyl)₃ ⁺X^~, -NH(Ci_6 alkyl)₂ ⁺X ^" -NH₂(Ci_₅ alkyl) ⁺X ^" -NH₃ ⁺X , - (OCi^ alkyl)(Ci_6 alkyl), -N(OH)(Ci_ 6 alkyl), -NH(OH), -SH, -SCue alkyl, -SS(Ci_6 alkyl),

alkyl), -C0₂H, -C0₂(Ci_₆ alkyl),

₆ alkyl)₂, -

alkyl), -NHC(=0)( CMS alkyl), -N(Ci_₆ alkyl)C(=0)( Ci_e alkyl), - NHC0₂(Ci_6 alkyl),

alkyl), -NHC(=0)NH₂, -

alkyl)₂, -

alkyl), -C(=NH)NH₂, -OC(=NH)N(C_w alkyl)₂, -OC(NH)NH(Ci_₆ alkyl), - OC(NH)NH₂,

alkyl)₂, -NHC(=NH)NH₂ , alkyl), -S0₂N(Ci_₆ alkyl)₂, -S0₂NH(Ci_6 alkyl), -S0₂NH₂,-S0₂Ci_₆ alkyl, -S0₂OCi ₆ alkyl, -OS0₂Ci ₅ alkyl, - SOCi-6 alkyl, -Si(Ci_₆ alkyl)₃, -Osi(Ci_₆ alkyl)₃ -C(=S)N(C_M alkyl)₂, C(=S)NH(Ci_₆ alkyl), C(=S)NH₂,

alkyl), -C(=S)SCi__s alkyl, alkyl),

alkyl)₂, -OP(=0)(OCi ₆ alkyl)₂, alkyl, perhaloalkyl, C₂-6 alkenyl, C₂_₆ alkynyl, C₃_i₀ carbocyclyl, C₆_i₀ aryl, 3-10 membered heterocyclyl, 5-10 membered heteroaryl; or two R⁸⁸ substituents can be joined to form =0 or =S; wherein X^~ is a counterion.

[0080] "Halo" or "halogen" refers to fluorine (fluoro, -F), chlorine (chloro, -CI), bromine (bromo, -Br), or iodine (iodo, -I).

[0081] "Acyl" as used herein refers to a moiety selected from the group consisting of - C(=0)R^aa,-CHO, -COzR^, -C(=0)N(R^bb)₂, -C(=NR^bb)R^aa, -C(=NR^bb)OR^aa, -C(=NR^bb)N(R^bb)₂, -C(=0)NR^bbS0₂R^aa, -C(=S)N(R^bb)₂, -C(=0)SR^aa, and -C(=S)SR^aa, wherein R^aa and R^bb are as defined herein.

[0082] Nitrogen atoms can be substituted or unsubstituted as valency permits, and include primary, secondary, tertiary, and quartemary nitrogen atoms. Exemplary nitrogen atom substituents include, but are not limited to, hydrogen, -OH, -OR^aa, -N(R^CC)₂, -CN, -C(=0)R^aa, - C(=0)N(R^cc)₂, -COz ³³, -S0₂R^aa, -C(=NR^bb)R^aa, -C(=NR^cc)OR^aa, -C(=NR^CC)N(R^CC)₂, - S0₂N(R^cc)₂, -S0₂R^cc, -S0₂OR^∞, -SOR³*, -C(=S)N(R^CC)₂, -C(=0)SR^cc, -C(=S)SR^CC, -

P(=0)₂R^aa, -P(=0)(R^aa)₂, -P(=0)₂N(R^cc)₂, -P(=0)(NR^cc)₂, d_₁₀ alkyl, d_₁₀ perhaloalkyl, C2-10 alkenyl, C₂_io alkynyl, C3_io carbocyclyl, 3-14 membered heterocyclyl, Ce-u aryl, and 5-14 membered heteroaryl, or two R^cc groups attached to a nitrogen atom are joined to form a 3-14 membered heterocyclyl or 5-14 membered heteroaryl ring, wherein each alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 _Rdd

groups, and wherein R^aa, R^bb, R^cc, and R^dd are as defined above.

[0083] In certain embodiments, the substituent present on a nitrogen atom is a nitrogen protecting group (also referred to as an amino protecting group). Nitrogen protecting groups include, but are not limited to, -OH, -OR^aa, -N(R^CC)₂, -C(=0)R^aa, -C(=0)N(R^cc)₂, -COzR^, - S0₂R^aa, -C(=NR^cc)R^aa, -C(=NR^cc)OR^aa, -C(=NR^CC)N(R^CC)₂, -S0₂N(R^cc)₂, -S0₂R^cc, -S0₂OR^cc, - SOR^a\ -C(=S)N(R^CC)₂, -C(=0)SR^cc, -C(=S)SR^CC, Ci-io alkyl (e.g., aralkyl, heteroaralkyl), C2-10 alkenyl, C₂_io alkynyl, C3_io carbocyclyl, 3-14 membered heterocyclyl, C<s-i4 aryl, and 5-14 membered heteroaryl groups, wherein each alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aralkyl, aryl, and heteroaryl is independently substituted with 0, 1 , 2, 3, 4, or 5 R^dd groups, and wherein R^aa, R^bb, R^cc and R^dd are as defined herein. Nitrogen protecting groups are well known in the art and include those described in Protecting Groups in Organic Synthesis, T. W. Greene and P. G. M. Wuts, 3^rd edition, John Wiley & Sons, 1999, incorporated herein by reference.

[0084] For example, nitrogen protecting groups such as amide groups (e.g., -C(=0)R^aa) include, but are not limited to, formamide, acetamide, chloroacetamide, trichloroacetamide, trifluoroacetamide, phenylacetamide, 3-phenylpropanamide, picolinamide, 3- pyridylcarboxamide, N-benzoylphenylalanyl derivative, benzamide, /J-phenylbenzamide, o— nitophenylacetamide, o-nitrophenoxyacetamide, acetoacetamide, (N- dithiobenzyloxyacylamino)acetamide, 3-(/?-hydroxyphenyl)propanamide, 3-(o- nitrophenyl)propanamide, 2-methyl-2-(o-nitrophenoxy)propanamide, 2-methyl-2-(o- phenylazophenoxy)propanamide, 4-chlorobutanamide, 3-methyl-3-nitrobutanamide, o- nitrocinnamide, N-acetylmethionine derivative, o-nitrobenzamide, and o—

(benzoyloxymethyl)benzamide.

[0085] Nitrogen protecting groups such as carbamate groups (e.g., -C(=0)OR^aa) include, but are not limited to, methyl carbamate, ethyl carbamante, 9-fluorenylmethyl carbamate (Fmoc), 9-(2-sulfo)fluorenylmethyl carbamate, 9-(2,7-dibromo)fluoroenylmethyl carbamate, 2,7— di— i— butyl-[9-( 10,10-dioxo- 10, 10, 10, 10-tetrahydrothioxanthyl)]methyl carbamate (DBD-Tmoc), 4-methoxyphenacyl carbamate (Phenoc), 2,2,2-trichloroethyl carbamate (Troc), 2- trimethylsilylethyl carbamate (Teoc), 2-phenylethyl carbamate (hZ), 1-(1-Adamantyl)-1- methylethyl (Adpoc), l ,l-dimethyl-2-halo ethyl carbamate, l,l-dimethyl-2,2-dibromoethyl carbamate (DB-r-BOC), l , l-dimethyl-2,2,2-trichloroethyl carbamate (TCBOC), 1-methyl-l- (4-biphenylyl)ethyl carbamate (Bpoc), l-(3,5-di-i-butylphenyl)-l-methylethyl carbamate (t- Bumeoc), 2-(2'- and 4'-pyridyl)ethyl carbamate (Pyoc), 2-{N,N- dicyclohexylcarboxamido)ethyl carbamate, i-butyl carbamate (BOC), 1-adamantyl carbamate (Adoc), vinyl carbamate (Voc), allyl carbamate (Alloc), 1-isopropylallyl carbamate (Ipaoc), cinnamyl carbamate (Coc), 4-nitrocinnamyl carbamate (Noc), 8-quinolyl carbamate, N- hydroxypiperidinyl carbamate, alkyldithio carbamate, benzyl carbamate (Cbz), p- methoxybenzyl carbamate (Moz), /)-nitobenzyl carbamate, p-bromobenzyl carbamate, p- chlorobenzyl carbamate, 2,4-dichlorobenzyl carbamate, 4-methylsulfinylbenzyl carbamate (Msz), 9-anthrylmethyl carbamate, diphenylmethyl carbamate, 2-methylthioethyl carbamate, 2- methylsulfonylethyl carbamate, 2-(/?-toluenesulfonyl)ethyl carbamate, [2— (1 ,3—

dithianyl)]methyl carbamate (Dmoc), 4-methylthiophenyl carbamate (Mtpc), 2,4- dimethylthiophenyl carbamate (Bmpc), 2-phosphonioethyl carbamate (Peoc), 2- triphenylphosphonioisopropyl carbamate (Ppoc), l , l-dimethyl-2-cyanoethyl carbamate, m- chloro-p-acyloxybenzyl carbamate, /?-(dihydroxyboryl)benzyl carbamate, 5- benzisoxazolylmethyl carbamate, 2-(trifluoromethyl)-6-chromonylmethyl carbamate (Tcroc), m-nitrophenyl carbamate, 3,5-dimethoxybenzyl carbamate, o-nitrobenzyl carbamate, 3,4- dimethoxy-6-nitrobenzyl carbamate, phenyl(o-nitrophenyl)methyl carbamate, f-amyl carbamate, S-benzyl thiocarbamate, 7-cyanobenzyl carbamate, cyclobutyl carbamate, cyclohexyl carbamate, cyclopentyl carbamate, cyclopropylmethyl carbamate, /?-decyloxybenzyl carbamate, 2,2-dimethoxyacylvinyl carbamate, o-(NN-dimethylcarboxamido)benzyl carbamate, 1 , l-dimethyl-3-(N,N-dimethylcarboxamido)propyl carbamate, 1 , 1-dimethylpropynyl carbamate, di(2-pyridyl)methyl carbamate, 2-furanylmethyl carbamate, 2-iodoethyl carbamate, isoborynl carbamate, isobutyl carbamate, isonicotinyl carbamate, p-(p'- methoxyphenylazo)benzyl carbamate, 1-methylcyclobutyl carbamate, 1-methylcyclohexyl carbamate, 1 -methyl- 1 -cyclopropylmethyl carbamate, l-methyl-l-(3,5- dimethoxyphenyl)ethyl carbamate, 1 -methyl- l-( ?-phenylazophenyl)ethyl carbamate, 1- methyl-l-phenylethyl carbamate, 1 -methyl- l-(4-pyridyl)ethyl carbamate, phenyl carbamate,?-(phenylazo)benzyl carbamate, 2,4,6-tri-i-butylphenyl carbamate, 4- (trimethylammonium)benzyl carbamate, and 2,4,6-trimethylbenzyl carbamate.

[0086] Nitrogen protecting groups such as sulfonamide groups (e.g., -S(=0)₂R^aa) include, but are not limited to, 7-toluenesulfonamide (Ts), benzenesulfonamide, 2,3,6,-trimethyl-4- methoxybenzenesulfonamide (Mtr), 2,4,6-trimethoxybenzenesulfonamide (Mtb), 2,6-dimethyl- 4-methoxybenzenesulfonamide (Pme), 2,3,5, 6-tetramethyl-4-methoxybenzenesulfonamide (Mte), 4-methoxybenzenesulfonamide (Mbs), 2,4,6-trimethylbenzenesulfonamide (Mts), 2,6- dimethoxy- -methylbenzenesulfonamide (iMds), 2,2,5, 7,8-pentamethylchroman-6- sulfonamide (Pmc), methanesulfonamide (Ms), β-trimethylsilylethanesulfonamide (SES), 9- anthracenesulfonamide, 4-(4',8'-dimethoxynaphthylmethyl)benzenesulfonamide (DNMBS), benzylsulfonamide, trifluoromethylsulfonamide, and phenacylsulfonamide.

[0087] Other nitrogen protecting groups include, but are not limited to, phenothiazinyl-(lO)- acyl derivative, V- 5-toluenesulfonylaminoacyl derivative, N-phenylaminothioacyl derivative, N-benzoylphenylalanyl derivative, N-acetylmethionine derivative, 4,5-diphenyl-3-oxazolin-2- one, N-phthalimide, N-dithiasuccinimide (Dts), N-2,3-diphenylmaleimide, N-2,5- dimethylpyrrole, N-l ,l ,4,4-tetramethyldisilylazacyclopentane adduct (STABASE), 5- substituted l ,3-dimethyl-l,3,5-triazacyclohexan-2-one, 5-substituted l,3-dibenzyl-l,3,5- triazacyclohexan-2-one, 1 -substituted 3,5-dinitro-4-hydroxyl, N-methylamine, N-allylamine, N-[2-(trimethylsilyl)ethoxy]methylamine (SEM), N-3-acetoxypropylamine, N-(l-isopropyl- 4-nitro-2-oxo-3-pyroolin-3-yl)amine, quaternary ammonium salts, N-benzylamine, N-di(4- methoxyphenyl)methylamine, N-5-dibenzosuberylamine, N-triphenylmethylamine (Tr), N— [(4— methoxyphenyl)diphenylmethyl]amine (MMTr), N-9-phenylfluorenylamine (PhF), N— 2,7— dichloro-9-fluorenylmethyleneamine, N-ferrocenylmethylamino (Fcm), N-2-picolylamino N- oxide, N-l,l-dimethylthiomethyleneamine, N-benzylideneamine, N-p- methoxybenzylideneamine, N-diphenylmethyleneamine, N-[(2- pyridyl)mesityl]methyleneamine, N-(N,N-dimethylaminomethylene)amine, Ν,Ϊ - isopropylidenediamine, N-p-nitrobenzylideneamine, N-salicylideneamine, N-5- chlorosalicylideneamine, N-(5-chloro-2-hydroxyphenyl)phenylmethyleneamine, N- cyclohexylideneamine, N-(5,5-dimethyl-3-oxo-l-cyclohexenyl)amine, N-borane derivative, N-diphenylborinic acid derivative, N-[phenyl(pentaacylchromium- or tungsten)acyl] amine, N- copper chelate, N-zinc chelate, N-nitroamine, N-nitrosoamine, amine N-oxide,

diphenylphosphmamide (Dpp), dimethylthiophosphinamide (Mpt), diphenylthiophosphmamide (Ppt), dialkyl phosphoramidates, dibenzyl phosphoramidate, diphenyl phosphoramidate, benzenesulfenamide, o-nitrobenzenesulfenamide (Nps), 2,4-dinitrobenzenesulfenamide, pentachlorobenzenesulfenamide, 2-nitro^-methoxybenzenesulfenamide,

triphenylmethylsulfenamide, and 3-nitropyridinesulfenamide ( pys).

[0088] In certain embodiments, the substituent present on an oxygen atom is an oxygen protecting group (also referred to as a hydroxyl protecting group). Oxygen protecting groups include, but are not limited to, -R^aa, -N(R^bb)₂, -C(=0)SR^aa, -C(=0)R^aa, -C0₂R^aa, - C(=0)N(R^bb)₂, -C(=NR^bb)R^aa, -C(=NR^bb)OR^aa, -C(=NR^bb)N(R^bb)₂, -S(=0)R^aa, -S0₂R^aa, - Si(R^aa)₃ -P(R^CC)₂, -P(R^CC)₃, -P(=0)₂R^aa, -P(=0)(R^aa)₂, -P(=0)(OR^cc)₂, -P(=0)₂N(R^bb)₂, and - P(=0)(NR^bb)₂, wherein R^aa, R^bb, and R^cc are as defined herein. Oxygen protecting groups are well known in the art and include those described in Protecting Groups in Organic Synthesis, T. W. Greene and P. G. M. Wuts, 3^rd edition, John Wiley & Sons, 1999, incorporated herein by reference.

[0089] Exemplary oxygen protecting groups include, but are not limited to, methyl, methoxylmethyl (MOM), methylthiomethyl (MTM), i-butylthiomethyl,

(phenyldimethylsilyl)methoxymethyl (SMOM), benzyloxymethyl (BOM), /?- methoxybenzyloxymethyl (PMBM), (4-methoxyphenoxy)methyl (p-AOM), guaiacolmethyl (GUM), i-butoxymethyl, 4-pentenyloxymethyl (POM), siloxymethyl, 2-methoxyethoxymethyl (MEM), 2,2,2-trichloroethoxymethyl, bis(2-chloroethoxy)methyl, 2-

(trimethylsilyl)ethoxymethyl (SEMOR), tetrahydropyranyl (THP), 3-bromotetrahydropyranyl, tetrahydrothiopyranyl, 1-methoxycyclohexyl, 4-methoxytetrahydropyranyl (MTHP), 4- methoxytetrahydrothiopyranyl, 4-methoxytetrahydrothiopyranyl S,S-dioxide, l-[(2-chloro-4- methyl)phenyl]-4-methoxypiperidin-4-yl (CTMP), l,4-dioxan-2-yl, tetrahydrofuranyl, tetrahydrothiofuranyl, 2,3,3a,4,5,6,7,7a-octahydro-7,8,8-trimethyl-4,7-methanobenzofuran-2- yl, 1-ethoxy ethyl, l-(2-chloroethoxy)ethyl, 1-methyl-l-methoxyethyl, 1-methyl-l- benzyloxy ethyl, 1 -methyl- l-benzyloxy-2-fluoroethyl, 2,2,2-trichloroethyl, 2- trimethylsilylethyl, 2-(phenylselenyl)ethyl, i-butyl, allyl, >-chlorophenyl, jo-methoxyphenyl, 2,4-dinitrophenyl, benzyl (Bn), /?-methoxybenzyl, 3,4-dimethoxybenzyl, o-nitrobenzyl, p- nitrobenzyl, /j-halobenzyl, 2,6-dichlorobenzyl, p-cyanobenzyl, /j-phenylbenzyl, 2-picolyl, 4- picolyl, 3-methyl-2-picolyl N-oxido, diphenylmethyl, p,p -dinitrobenzhydryl, 5- dibenzosuberyl, triphenylmethyl, a-naphthyldiphenylmethyl, /?-methoxyphenyldiphenylmethyl, di(p-methoxyphenyl)phenylmethyl, tri(p-methoxyphenyl)methyl, 4-(4'- bromophenacyloxyphenyl)diphenylmethyl, 4,4',4"-tris(4,5-dichlorophthalimidophenyl)methyl, 4,4^,,4"-tris(levulinoyloxyphenyl)methyl, 4,4^r,4"-tris(benzoyloxyphenyl)methyl, 3-(imidazol-l- yl)bis(4',4"-dimethoxyphenyl)methyl, 1 , l-bis(4-methoxyphenyl)-l '-pyrenylmethyl, 9-anthryl, 9-(9-phenyl)xanthenyl, 9-(9-phenyl-l 0-oxo)anthryl, 1 ,3-benzodithiolan-2-yl,

benzisothiazolyl S,S-dioxido, trimethylsilyl (TMS), triethylsilyl (TES), triisopropylsilyl (TIPS), dimethylisopropylsilyl (IPDMS), diethylisopropylsilyl (DEIPS), dimethylthexylsilyl, t- butyldimethylsilyl (TBDMS), i-butyldiphenylsilyl (TBDPS), tribenzylsilyl, tri-p-xylylsilyl, triphenylsilyl, diphenylmethylsilyl (DPMS), i-butylmethoxyphenylsilyl (TBMPS), formate, benzoylformate, acetate, chloroacetate, dichloroacetate, trichloroacetate, trifluoroacetate, methoxyacetate, triphenylmethoxyacetate, phenoxyacetate, /?-chlorophenoxyacetate, 3- phenylpropionate, 4-oxopentanoate (levulinate), 4,4-(ethylenedithio)pentanoate

(levulinoyldithioacetal), pivaloate, adamantoate, crotonate, 4-methoxycrotonate, benzoate, >- phenylbenzoate, 2,4,6-trimethylbenzoate (mesitoate), methyl carbonate, 9-fluorenylmethyl carbonate (Fmoc), ethyl carbonate, 2,2,2-trichloroethyl carbonate (Troc), 2-(trimethylsilyl)ethyl carbonate (TMSEC), 2-(phenylsulfonyl) ethyl carbonate (Psec), 2-(triphenylphosphonio) ethyl carbonate (Peoc), isobutyl carbonate, vinyl carbonate, allyl carbonate, i-butyl carbonate (BOC), p-nitrophenyl carbonate, benzyl carbonate, /p-methoxybenzyl carbonate, 3,4-dimethoxybenzyl carbonate, o-nitrobenzyl carbonate, jo-nitrobenzyl carbonate, S-benzyl thiocarbonate, 4- ethoxy-l-napththyl carbonate, methyl dithiocarbonate, 2-iodobenzoate, 4-azidobutyrate, 4- nitro-4-methylpentanoate, o-(dibromomethyl)benzoate, 2-formylbenzenesulfonate, 2- (methylthiomethoxy)ethyl, 4-(methylthiomethoxy)butyrate, 2-

(methylthiomethoxymethyl)benzoate, 2,6-dichloro-4-methylphenoxyacetate, 2,6-dichloro-4- (l ,l,3,3-tetramethylbutyl)phenoxyacetate, 2,4-bis(l , l-dimethylpropyl)phenoxyacetate, chlorodiphenylacetate, isobutyrate, monosuccinoate, (£)-2-methyl-2-butenoate, o- (methoxyacyl)benzoate, a-naphthoate, nitrate, alkyl N,NN',N'-tetramethylphosphorodiamidate, alkyl N-phenylcarbamate, borate, dimethylphosphinothioyl, alkyl 2,4-dinitrophenylsulfenate, sulfate, methanesulfonate (mesylate), benzylsulfonate, and tosylate (Ts).

[0090] In certain embodiments, the substituent present on a sulfur atom is an sulfur protecting group (also referred to as a thiol protecting group). Sulfur protecting groups include, but are not limited to, -R^aa, -N(R^bb)₂, -C(=0)SR^aa, -C(=0)R^aa, -C0₂R^aa, -C(=0)N(R^bb)₂, -C(=NR^bb)R^aa, - C(=NR^bb)OR^aa, -C(=NR^bb)N(R^bb)₂, -S(=0)R^aa, -S0₂R^aa, -SiC . -P(R^CC)₂, -P(R^CC)₃, - P(=0)₂R^aa, -P(=0)(R^aa)₂, -P(=0)(OR^cc)₂, -P(=0)₂N(R^bb)₂, and -P(=0)( R^bb)₂, wherein R^aa, R^bb, and R^cc are as defined herein. Sulfur protecting groups are well known in the art and include those described in Protecting Groups in Organic Synthesis, T. W. Greene and P. G. M. Wuts, 3^rd edition, John Wiley & Sons, 1999, incorporated herein by reference.

[0091] As used herein, the term "leaving group" is given its ordinary meaning in the art of synthetic organic chemistry and refers to an atom or a group capable of being displaced by a nucleophile. Examples of suitable leaving groups include, but are not limited to, halogen (such as F, CI, Br, or I (iodine)), alkoxycarbonyloxy, aryloxycarbonyloxy, alkanesulfonyloxy, arenesulfonyloxy, alkyl-carbonyloxy (e.g., acetoxy), arylcarbonyloxy, aryloxy, methoxy, Ν,Ο- dimethylhydroxylamino, pixyl, and haloformates. In some cases, the leaving group is a sulfonic acid ester, such as toluenesulfonate (tosylate, -OTs), methanesulfonate (mesylate, -OMs), p- bromobenzenesulfonyloxy (brosylate, -OBs), or trifluoromethanesulfonate (triflate, -OTf). In some cases, the leaving group is a brosylate, such as p-bromobenzenesulfonyloxy. In some cases, the leaving group is a nosylate, such as 2-nitrobenzenesulfonyloxy. In some embodiments, the leaving group is a sulfonate-containing group. In some embodiments, the leaving group is a tosylate group. The leaving group may also be a phosphineoxide (e.g. , formed during a

Mitsunobu reaction) or an internal leaving group such as an epoxide or cyclic sulfate. Other non- limiting examples of leaving groups are water, ammonia, alcohols, ether moieties, thioether moieties, zinc halides, magnesium moieties, diazonium salts, and copper moieties.

General Definitions

[0092] The following definitions are more general terms used throughout the present application:

[0093] As used herein, a"carbohydrate group" or a "carbohydrate" refers to a monosaccharide or a polysaccharide (e.g., a disaccharide or oligosaccharide). Exemplary monosaccharides include, but are not limited to, natural sugars, such as allose, altrose, glucose, mannose, gulose, idose, galactose, talose, ribose, arabinose, xylose, and lyxose. Disaccharides are two joined monosaccharides. Exemplary disaccharides include, but are not limited to, sucrose, maltose, cellobiose, and lactose. Typically, an oligosaccharide includes between three and ten monosaccharide units (e.g. , raffmose, stachyose). The carbohydrate group may be a natural sugar or a modified sugar. Exemplary modified sugars include, but are not limited to, sugars where the hydroxyl group is replaced with an amino group and/or alkyl group {e.g., such as desosamine), 2 -deoxyribose wherein a hydroxyl group is removed, 2 -fluororibose wherein a hydroxyl group is replace with a fluorine, or N-acetylglucosamine, or a nitrogen-containing form of glucose (e.g., 2 -fluororibose, deoxyribose, and hexose), and the like. Various carbohydrates are further described below and herein. Carbohydrates may exist in many different forms, for example, conformers, cyclic forms, acyclic forms, stereoisomers, tautomers, anomers, and isomers.

[0094] As used herein, the term "conjugated" or "conjugation" refers to an association of two molecules, for example, a glycan moiety and a protein carrier, with one another in a way that they are linked by a direct or indirect covalent or non-covalent interaction. In certain embodiments, the association is covalent, and the entities are said to be "conjugated" to one another.

[0095] As used herein, the term "pharmaceutically acceptable salt" refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, Berge et a!., describe pharmaceutically acceptable salts in detail in g. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference.

Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid, and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid, or malo ic acid or by using other methods known in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate,

benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxyl-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, /j-toluenesulfonate, undecanoate, valerate salts, and the like. Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N (Ci_4 alkyl)4 salts. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, non-toxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate, and aryl sulfonate.

[0096] A "subject" to which administration is contemplated includes, but is not limited to, humans (i.e., a male or female of any age group, e.g. , a pediatric subject (e.g., infant, child, adolescent) or adult subject (e.g., young adult, middle-aged adult, or senior adult)) and/or other non-human animals, for example, mammals (e.g. , primates (e.g. , cynomolgus monkeys, rhesus monkeys); commercially relevant mammals such as cattle, pigs, horses, sheep, goats, cats, and/or dogs) and birds (e.g. , commercially relevant birds such as chickens, ducks, geese, and/or turkeys). In certain embodiments, the animal is a mammal. The animal may be a male or female and at any stage of development. A non-human animal may be a transgenic animal.

[0097] The terms "administer," "administering," or "administration," as used herein refers to implanting, absorbing, ingesting, injecting, inhaling, or otherwise introducing an inventive compound, or a pharmaceutical composition thereof.

[0098] As used herein, the terms "treatment," "treat," and "treating" refer to reversing, alleviating, delaying the onset of, or inhibiting the progress of a "pathological condition" (e.g., a disease, disorder, or condition, or one or more signs or symptoms thereof) described herein. In some embodiments, treatment may be administered after one or more signs or symptoms have developed or have been observed. In other embodiments, treatment may be administered in the absence of signs or symptoms of the disease or condition. For example, treatment may be administered to a susceptible individual (e.g., an individual at risk for the disease) prior to the onset of symptoms (e.g., in light of a history of symptoms and/or in light of genetic or other susceptibility factors). Treatment may also be continued after symptoms have resolved, for example, to delay or prevent recurrence.

[0099] As used herein, the terms "condition," "disease," and "disorder" are used

interchangeably.

[0100] An "effective amount" of a compound refers to an amount sufficient to elicit a desired biological response, i.e., treating the condition. As will be appreciated by those of ordinary skill in this art, the effective amount of a compound may vary depending on such factors as the desired biological endpoint, the pharmacokinetics of the compound, the condition being treated, the mode of administration, and the age and health of the subject. An effective amount encompasses therapeutic and prophylactic treatment. In certain embodiments, the effective amount encompasses an amount effective in eliciting an immune responses specific to a bacterial infectious disease. In certain embodiments, the effective amount encompasses an amount effective in eliciting an immune responses specific to an infectious disease caused by a gram-negative bacterium. In certain embodiments, the effective amount encompasses an amount effective in eliciting an immune responses specific to an infectious disease caused by Neisseria meningitides. In certain embodiments, the effective amount encompasses an amount effective in eliciting an immune responses specific to an infectious disease caused by Neisseria meningitides serogroup W135.

[0101] A "therapeutically effective amount" of a compound is an amount sufficient to provide a therapeutic benefit in the treatment of a condition or to delay or minimize one or more symptoms associated with the condition. A therapeutically effective amount of a compound means an amount of therapeutic agent, alone or in combination with other therapies, which provides a therapeutic benefit in the treatment of the condition. The term "therapeutically effective amount" can encompass an amount that improves overall therapy, reduces or avoids symptoms or causes of the condition, or enhances the therapeutic efficacy of another therapeutic agent.

[0102] A "prophylactically effective amount" of a compound is an amount sufficient to prevent a condition, or one or more symptoms associated with the condition or prevent its recurrence. A prophylactically effective amount of a compound means an amount of a therapeutic agent, alone or in combination with other agents, which provides a prophylactic benefit in the prevention of the condition. The term "prophylactically effective amount" can encompass an amount that improves overall prophylaxis or enhances the prophylactic efficacy of another prophylactic agent.

[0103] In certain embodiments, a compound of the present invention is provided as a salt. Salts are well known in the art. For example, Berge et al., describe pharmaceutically acceptable salts in detail in J Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference. Salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. Examples include salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malo ic acid or by using other methods used in the art such as ion exchange. Other salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxyl-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2- naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N (Ci_ ₄alkyl)₄ salts. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further salts include, when appropriate, ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate.

[0104] As used herein "inhibition," "inhibiting," and "inhibit", refer to the ability of a compound to reduce, slow, halt or prevent activity of a particular biological process relative to a control vehicle or absence of the compound (e.g. , by at least 20%, 50%, 80%, 90%, 95%, or 100%). In certain embodiments, the biological process is in vitro (e.g., cellular assay). In certain embodiments, the biological process is in vivo. In certain embodiments, a probe compound of the present invention inhibits a glycosyltransferase protein.

[0105] The term "independently" is used herein to indicate that the groups can be identical or different.

[0106] As used herein, the term "antigen" is defined as any substance capable of eliciting an immune response: e.g., a B-cell response or a T-cell response.

[0107] As used herein, the term "immunogenicity" refers to the ability of an immunogen, antigen, or vaccine to stimulate an immune response.

[0108] As used herein, the term "immunotherapy" refers to an array of treatment strategies based upon the concept of modulating the immune system to achieve a prophylactic and/or therapeutic goal. [0109] As used herein, the term "epitope" is defined as the parts of an antigen molecule which contact the antigen binding site of an antibody or a T cell receptor.

[0110] As used herein, the term "immunogenic composition" refers to a preparation that contains an antigen, such as proteins, peptides, lipid, polysaccharides, or whole disease-causing organisms (killed or weakened), and capable of elicit an immune response specific to the antigen. An immunogenic composition (e.g., a vaccine) may be used to confer immunity against a disease associated with the antigen, e.g. , against an infection caused by an microorganism from which the antigen is derived. An immunogenic composition or the antigen contained therein can be natural, synthetic, or prepared by recombinant DNA technology.

[0111] As used herein, the term "adjuvant" refers to a substance capable of modifying (e.g., enhancing) the immune response to an immunogen when it is used in conjunction with the immunogen. An adjuvant may not elicit immune responses specific to itself. In some examples, the a-GalCer analogs described herein (e.g., C34) are used as adjuvants to modify or augment the effects of an immunogen by stimulating the immune system of a patient who is administered the immunogen to respond to the immunogen more vigorously. In other examples, the adjuvant can be an aluminum salt.

[0112] As used herein, the term "antigen specific" or "immunogen specific" refers to a property of a cell population such that supply of a particular antigen or immunogen, or a fragment thereof, results in specific cell proliferation.

[0113] The present invention provides synthetic glycan conjugates comprising oligosaccharide antigens derived from Neisseria meningitidis (e.g., serogroup W135), immmunogenic compositions thereof, and kits thereof, as well as methods of synthesizing the oligosaccharide antigens and glycan conjugates and methods of using such for eliciting immune responses specific to the oligosaccharide antigens and treating or reducing the risk for infectious diseases (e.g., bacterial infections such as those caused by Neisseria meningitidis).

Compounds

[0114] The present invention provides carbohydrate compounds of Formula (F-l):

(F-l) or a salt thereof,

1 2 3 4 5 6 V

wherein each instance o R , R , R , R , R , R , and R is independently hydrogen, optionally substituted Ci_6 alkyl, or an oxygen protecting group; or optionally R¹ and R² are taken with the intervening atoms to form a heterocyclic ring; or optionally R² and R³ are taken with the intervening atoms to form a heterocyclic ring; or optionally R⁵ and R⁶ are taken with the intervening atoms to form a heterocyclic ring; or R^N1 and R⁰¹ are taken together with the intervening atoms to form a heterocyclic ring; each instance of R^N1 and R^N2 is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group; each instance of R⁰¹ is independently hydrogen, optionally substituted Ci_6 alkyl, or an oxygen protecting group; B is -N3 or N(R^BN)2; each instance of R^BN is independently hydrogen, optionally substituted Ci_₆ alkyl, optionally substituted acyl, or a nitrogen protecting group; n is an integer of 1 to 100, inclusive; and p is an integer of 1 to 10, inclusive.

[0115] As generally defined herein, B is -N3 or N(R^B )2, wherein each instance of R^BN is independently hydrogen, optionally substituted Ci_e alkyl, or a nitrogen protecting group. In some embodiments, B is -N3. In some embodiments, B is N¾. In some embodiments, B is NHR^BN, wherein R^BN is optionally substituted Ci_₆ alkyl. In some embodiments, B is NHR^BN, wherein R^BN is methyl, ethyl, or propyl. In some embodiments, B is NHR^BN, wherein R^BNis a nitrogen protecting group. In some embodiments, B is NHR^BN, wherein R^BN is Ac, Bn, BOC, Cbz, Troc, Fmoc, trifluoroacetyl, triphenylmethyl, or Ts. In some embodiments, B is N(R^BN)2, wherein R^BN are the same. In some embodiments, B is N(R^BN)2, wherein R^BN are different. In some embodiments, B is N(R^B )2, wherein each instance of R^BN is Ac, Bn, BOC, Cbz, Troc, Fmoc, trifluoroacetyl, triphenylmethyl, or Ts. In some embodiments, B is NBnR^BN, wherein R^BN is Ac, Bn, BOC, Cbz, Troc, Fmoc, trifluoroacetyl, triphenylmethyl, or Ts. In some

embodiments, B is NBnCbz.

[0116] As generally defined herein, p is an integer of 1 to 10, inclusive. In certain embodiments, p is 1. In certain embodiments, p is 2. In certain embodiments, p is 3. In certain embodiments, p is 4. In certain embodiments, p is 5. In certain embodiments, p is 6. In certain embodiments, p is 7. In certain embodiments, p is 8. In certain embodiments, p is 9. In certain embodiments, p is 10.

[0117] As used herein, R¹, R², R³, R⁴, R⁵, R⁶, and R⁷ are as defined herein.

[0118] In certain embodiments, the carbohydrate compounds provided herein are of Formula (F-

2):

(F-2)

or a salt thereof.

[0119] In other embodiments, ths carbohydrate compounds provided herein are of Formula (F- 3):

(F-3)

or a salt thereof, wherein each instance of R² and R^2b is independently optionally substituted Cl-6 alkyl. In certain embodiments, R^2a is unsubstituted Cl-6 alkyl. In certain embodiments, R^' is methyl, ethyl, or propyl. In certain embodiments, R^2b is unsubstituted Cl-6 alkyl. In certain embodiments, R^2b is methyl, ethyl, or propyl.

[0120] Alternatively, the carbohydrate compounds described herein can have the structure of Formula (F-4):

(F-4)

or a salt thereof. [0121] As used herein, n is an integer of 1 to 100, inclusive. In certain embodiments, n is an integer of 1 to 80, inclusive. In certain embodiments, n is an integer of 1 to 60, inclusive. In certain embodiments, n is an integer of 1 to 40, inclusive. In certain embodiments, n is an integer of 1 to 20, inclusive. In certain embodiments, n is an integer of 1 to 10, inclusive. In certain embodiments, n is an integer of 1 to 8, inclusive. In certain embodiments, n is 1. In certain embodiments, n is 2. In certain embodiments, n is 3. In certain embodiments, n is 4. In certain embodiments, n is 5. In certain embodiments, n is 6. In certain embodiments, n is 7. In certain embodiments, n is 8.

[0122] Alternatively, the carbohydrate compounds described herein can have the structure of Formula (F-5):

(F-5)

or a salt thereof, wherein v is an integer from 1 to 99, inclusive.

Glycan conjugates

[0123] The present invention provides a glycan conjugate or a pharmaceutically acceptable salt thereof, comprising a carrier and a glycan moiety of Formula (I):

(I)

wherein: the glycan moiety is covalently linked to the carrier through a linker -L-; each instance of R¹, R², R³, R⁴, R⁵, R⁶, and R⁷ is independently hydrogen, optionally substituted Ci_6 alkyl, or an oxygen protecting group; or optionally R¹ and R² are taken with the intervening atoms to form a heterocyclic ring; or optionally R² and R³ are taken with the intervening atoms to form a heterocyclic ring; or optionally R⁵ and R⁶ are taken with the intervening atoms to form a heterocyclic ring; or optionally R⁶ and R⁷ are taken with the intervening atoms to form a heterocyclic ring; or R^N1 and R⁰¹ are taken together with the intervening atoms to form a heterocyclic ring; each instance of R^N1, R^N2, and R^BN is independently hydrogen, optionally substituted Ci_₆ alkyl, optionally substituted acyl, or a nitrogen protecting group; each instance of R⁰¹ is independently hydrogen, optionally substituted Ci_₆ alkyl, or an oxygen protecting group; each instance of L is independently a bond, -C(=0)-, -C(=0)NR^La-, -C(=0)S-, -

-C≡C- -C(R^LB)₂0- - C(R^Lb)₂NR^L -, -C(R^Lb)₂S-, -S(=0)₂0- -S(=0)₂NR^La- or an optionally substituted Ci-₃₀ hydrocarbon chain, optionally wherein one or more carbon units of the hydrocarbon chain is replaced with -0-, -S-, -NR^La- -C(=0)-, NR^LaC(=0)-, -NR^LaC(=0)0- -C(=0)NR^La-, - OC(=0)NR^La-, -SC(=0)-, -C(=0)S-, -OC(=0)-, -C(=0)0-, -NR^LaC(=S)-, -C(=S)NR^La- trans-CR^Lb=CR^Lb- c«-CR^Lb=CR^Lb- -C≡C-, -S(=0)₂0-, -OS(=0)₂- -S(=0)₂NR^La- or - NR^LaS(=0)₂-, wherein each instance of R^La is hydrogen, optionally substituted CM_S alkyl, or a nitrogen protecting group, or R^La is joined with the adjacent carbon atom to form an optionally substituted heterocyclic ring, and wherein each occurrence of R^Lb is independently selected from the group consisting of hydrogen, halogen, optionally substituted Ci-₁₅ alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl, or R^Lb is joined with the adjacent carbon or nitrogen or oxygen atom to form an optionally substituted carbocyclic or heterocyclic ring, or two R^Lb groups are joined to form an optionally substituted carbocyclic or optionally substituted heterocyclic ring; p is an integer of 1 to 10, inclusive; and n is an integer of 1 to 100, inclusive.

[012

(I-a)

wherein each instance of L¹ is independently a bond, -0-, -S-, -NR^Lla-, -C(=0)-, -

-SC(=0)-, -C(=0)S- - OC(=0)-, -C(=0)0- -NR^LlaC(=S)-, -C(=S)NR^Lla-, ira¾s-CR^Llb=CR^Llb- cw-CR^Llb=CR^Llb- -C≡C-, -OC(R^Llb)₂-, -C(R^Llb)₂0-, -NR^LlaC(R^Llb)₂-, -C(R^Llb)₂NR^Lla-, -SC(R^Llb)₂- - C(R^Llb)₂S-, S(=0)₂0 , OS(=0)₂ , -S(=0)₂NR^Lla-, -NR^LlaS(=0)₂-, or an optionally substituted Ci_₂o hydrocarbon chain, optionally wherein one or more carbon units of the hydrocarbon chain is replaced with -0-, -S-, -NR^L1 -C(=0)-, NR^LlaC(=0)-, - NR^LlaC(=0)0- -C(=0)NR^Lla- -OC(=0)NR^Lla-, -SC(=0)-, -C(=0)S-, -OC(=0)-, - C(=0)0- -NR^LlaC(=S)-, -C(=S)NR^Lla-, fra«s-CR^Llb=CR^Llb- czs-CR^Llb=CR^Llb- -C≡C-, - S(=0)₂0- -OS(=0)₂- -S(=0)₂NR^Lla- or -NR^LlaS(=0)₂- wherein each instance of R^Lla is hydrogen, optionally substituted Ci^ alkyl, or a nitrogen protecting group, or R^Lla is joined with the adjacent carbon atom to form an optionally substituted heterocyclic ring, and wherem each occurrence of R^Llb is independently selected from the group consisting of hydrogen, halogen, optionally substituted Ci-io alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl, or R^Llb is joined with the adjacent carbon or nitrogen or oxygen atom to form an optionally substituted carbocyclic or heterocyclic ring, or two R^Llb groups are joined to form an optionally substituted carbocyclic or optionally substituted heterocyclic ring; each instance of L² is independently a moiety derived from a crosslinking

1 3

reagent capable of crosslinking the carrier and L -H; each instance of L is independently a crosslinking reagent capable of crosslinking the carrier and ΐ -Η; each instance of R^CN is independently hydrogen, optionally substituted Ci_₆ alkyl, optionally substituted acyl, or a nitrogen protecting group; w is a integer of 1 to 100, inclusive; and y is 0 or an integer of 1 to 100, inclusive.

[0125] In some embodiments, R may be hydrogen, one of R^N1 and R^N2 may be hydrogen, and/or one or more of R², R³' R⁵, and R⁶ are hydrogen. In some examples, R^N1 and R^N2 may be hydrogen and acetyl, respectively, or vise versa. In other examples, R⁷ is hydrogen. Examples of the glycan conjugates described herein include, but are not limited to Formular (II) and Formula (III) described herein, or pharmaceutically acceptable salts thereof.

[0126] As generally defined herein, R¹ is independently hydrogen, optionally substituted Ci_e alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R¹ is hydrogen. In certain embodiments, R¹ is optionally substituted Ci_6 alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R¹ is optionally substituted Ci_6 alkyl. In certain embodiments, R¹ is unsubstituted Ci_6 alkyl. In certain embodiments, R¹ is methyl, ethyl, or propyl. In certain embodiments, R¹ is optionally substituted carbohydrate as defined herein. In certain embodiments, R¹ is an oxygen protecting group. In certain embodiments, R¹ is acyl. In certain embodiments, R¹ is acetyl or Bz. In certain embodiments, R¹ is Bn, PMB. In certain embodiments, R¹ is substituted silyl. In certain embodiments, R¹ is TBDPS.

[0127] As generally defined herein, R² is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R² is hydrogen. In certain embodiments, R² is optionally substituted Ci__e alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R² is optionally substituted Ci_6 alkyl. In certain embodiments, R² is unsubstituted Ci_6 alkyl. In certain embodiments, R² is methyl, ethyl, or propyl. In certain embodiments, R² is optionally substituted carbohydrate as defined herein. In certain embodiments, R² is an oxygen protecting group. In certain embodiments, R² is acyl. In certain embodiments, R² is acetyl or Bz. In certain embodiments, R² is Bn, PMB. In certain embodiments, R² is substituted silyl. In certain embodiments, R² is TBDPS.

[0128] As generally defined herein, R³ is independently hydrogen, optionally substituted Ci-g alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R³ is hydrogen. In certain embodiments, R³ is optionally substituted Ci_6 alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R³ is optionally substituted Ci_6 alkyl. In certain embodiments, R³ is unsubstituted Ci_6 alkyl. In certain embodiments, R³ is methyl, ethyl, or propyl. In certain embodiments, R³ is optionally substituted carbohydrate as defined herein. In certain embodiments, R³ is an oxygen protecting group. In certain embodiments, R³ is acyl. In certain embodiments, R³ is acetyl or Bz. In certain embodiments, R³ is Bn, PMB. In certain embodiments, R³ is substituted silyl. In certain embodiments, R³ is TBDPS.

[0129] As generally defined herein, R⁴ is independently hydrogen, optionally substituted Ci_₆ alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R⁴ is hydrogen. In certain embodiments, R⁴ is optionally substituted Ci_6 alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R⁴ is optionally substituted Ci_6 alkyl. In certain embodiments, R⁴ is unsubstituted Ci_6 alkyl. In certain embodiments, R⁴ is methyl, ethyl, or propyl. In certain embodiments, R⁴ is optionally substituted carbohydrate as defined herein. In certain embodiments, R⁴ is an oxygen protecting group. In certain embodiments, R⁴ is acyl. In certain embodiments, R⁴ is acetyl or Bz. In certain embodiments, R⁴ is Bn, PMB. In certain embodiments, R⁴ is substituted silyl. In certain embodiments, R⁴ is TBDPS.

[0130] As generally defined herein, R⁵ is independently hydrogen, optionally substituted Ci_e alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R⁵ is hydrogen. In certain embodiments, R⁵ is optionally substituted Ci_6 alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R⁵ is optionally substituted Ci_6 alkyl. In certain embodiments, R⁵ is unsubstituted Ci_6 alkyl. In certain embodiments, R⁵ is methyl, ethyl, or propyl. In certain embodiments, R⁵ is optionally substituted carbohydrate as defined herein. In certain embodiments, R⁵ is an oxygen protecting group. In certain embodiments, R⁵ is acyl. In certain embodiments, R⁵ is acetyl or Bz. In certain embodiments, R⁵ is Bn, PMB. In certain embodiments, R⁵ is substituted silyl. In certain embodiments, R⁵ is TBDPS.

[0131] As generally defined herein, R⁶ is independently hydrogen, optionally substituted Ci_s alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain

embodiments, R⁶ is hydrogen. In certain embodiments, R⁶ is optionally substituted Ci_6 alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R⁶ is optionally substituted Ci_6 alkyl. In certain embodiments, R⁶ is unsubstituted Ci_6 alkyl. In certain embodiments, R⁶ is methyl, ethyl, or propyl. In certain embodiments, R⁶ is optionally substituted carbohydrate as defined herein. In certain embodiments, R⁶ is an oxygen protecting group. In certain embodiments, R⁶ is acyl. In certain embodiments, R⁶ is acetyl or Bz. In certain embodiments, R⁶ is Bn, PMB. In certain embodiments, R⁶ is substituted silyl. In certain embodiments, R⁶ is TBDPS.

[0132] As generally defined herein, R⁷ is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain

embodiments, R⁷ is hydrogen. In certain embodiments, R⁷ is optionally substituted Ci_e alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R⁷ is optionally substituted Ci_₆ alkyl. In certain embodiments, R⁷ is unsubstituted Ci_₆ alkyl. In certain embodiments, R⁷ is methyl, ethyl, or propyl. In certain embodiments, R⁷ is optionally substituted carbohydrate as defined herein. In certain embodiments, R⁷ is an oxygen protecting group. In certain embodiments, R⁷ is acyl. In certain embodiments, R⁷ is acetyl or Bz. In certain embodiments, R⁷ is Bn, PMB. In certain embodiments, R⁷ is substituted silyl. In certain embodiments, R⁷ is TBDPS.

[0133] As generally defined herein, R⁰¹ is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain

embodiments, R⁰¹ is hydrogen. In certain embodiments, R⁰¹ is optionally substituted Ci_6 alkyl, optionally substituted carbohydrate, or an oxygen protecting group. In certain embodiments, R⁰¹ is optionally substituted Ci_e alkyl. In certain embodiments, R⁰¹ is unsubstituted Ci_s alkyl. In certain embodiments, R⁰¹ is methyl, ethyl, or propyl. In certain embodiments, R⁰¹ is optionally substituted carbohydrate as defined herein. In certain embodiments, R⁰¹ is an oxygen protecting group. In certain embodiments, R⁰¹ is acyl. In certain embodiments, R⁰¹ is acetyl or Bz. In certain embodiments, R is Bn, PMB. In certain embodiments, R is substituted silyl. In certain embodiments, R⁰¹ is TBDPS.

[0134] In certain embodiments, R¹ and R² are taken with the intervening atoms to form an optionally substituted heterocyclic ring. In certain embodiments, R¹ and R² are taken with the intervening atoms to form an optionally substituted 5-membered heterocyclic ring. In certain embodiments, R¹ and R² are taken with the intervening atoms to form an optionally substituted 5-membered heterocyclic ring with two oxygen. In certain embodiments, R¹ and R² are taken with the intervening atoms to form a di-methylated 5-membered heterocyclic ring with two oxygen. In certain embodiments, R¹ and R² are taken with the intervening atoms to form a 5-

membered heterocyclic ring of the formula , wherein R^ra and R^r are each independently optionally substituted Ci_6 alkyl. In certain embodiments, R¹ and R² are taken

with the intervening atoms to form a 5-membered heterocyclic ring of the formula

wherein R^ra and R^rb are each independently unsubstituted Ci_e alkyl. In certain embodiments, R¹ and R² are taken with the intervening atoms to form a 5-membered heterocyclic ring of the

formula

, wherein R^ra and R^r are each independently methyl, ethyl, or propyl.

[0135] In certain embodiments, R² and R³ are taken with the intervening atoms to form an optionally substituted heterocyclic ring. In certain embodiments, R² and R³ are taken with the intervening atoms to form an optionally substituted 5-membered heterocyclic ring. In certain embodiments, R² and R³ are taken with the intervening atoms to form an optionally substituted 5-membered heterocyclic ring with two oxygen. In certain embodiments, R² and R³ are taken with the intervening atoms to form a di-methylated 5-membered heterocyclic ring with two oxygen. In certain embodiments, R³ and R⁴ are taken with the intervening atoms to form a 5-

membered heterocyclic ring of the formula

, wherein R^ra and R^r are each independently optionally substituted Ci-s alkyl. In certain embodiments, R² and R³ are taken R^ra _Rrb with the intervening atoms to form a 5-membered heterocyclic ring of the formula

wherein R^ra and R^rb are each independently unsubstituted Ci_₆ alkyl. In certain embodiments, R² and R³ are taken with the intervening atoms to form a 5-membered heterocyclic ring of the

formula

, wherein R^ra and R^r are each independently methyl, ethyl, or propyl.

[0136] In certain embodiments, R⁵ and R⁶ are taken with the intervening atoms to form an optionally substituted heterocyclic ring. In certain embodiments, R⁵ and R⁶ are taken with the intervening atoms to form an optionally substituted 5-membered heterocyclic ring. In certain embodiments, R⁵ and R⁶ are taken with the intervening atoms to form an optionally substituted 5-membered heterocyclic ring with two oxygen. In certain embodiments, R⁵ and R⁶ are taken with the intervening atoms to form a di-methylated 5-membered heterocyclic ring with two oxygen. In certain embodiments, R⁵ and R⁶ are taken with the intervening atoms to form a 5-

membered heterocyclic ring of the formula

, wherein R^ra and R^r are each independently optionally substituted Ci_6 alkyl. In certain embodiments, R⁵ and R⁶ are taken

, wherein R^ra and R^rb are each independently unsubstituted Ci_6 alkyl. In certain embodiments, R and R⁶ are taken with the intervening atoms to form a 5-membered heterocyclic ring of the

formula

, wherein R^ra and R^r are each independently methyl, ethyl, or propyl.

[0137] In certain embodiments, R⁶ and R⁷ are taken with the intervening atoms to form an optionally substituted heterocyclic ring. In certain embodiments, R⁵ and R⁷ are taken with the intervening atoms to form an optionally substituted 5-membered heterocyclic ring. In certain embodiments, R⁶ and R⁷ are taken with the intervening atoms to form an optionally substituted 5-membered heterocyclic ring with two oxygen. In certain embodiments, R⁶ and R⁷ are taken with the intervening atoms to form a di-methylated 5-membered heterocyclic ring with two oxygen. In certain embodiments, R⁶ and R⁷ are taken with the intervening atoms to form a 5-

membered heterocyclic ring of the formula

, wherein R^ra and R^r are each

independently optionally substituted Ci_6 alkyl. In certain embodiments, R⁶ and R⁷ are taken

, wherein R^ra and R^rb are each independently unsubstituted Ci_6 alkyl. In certain embodiments, R and R⁷ are taken with the intervening atoms to form a 5-membered heterocyclic ring of the

formula

, wherein R^ra and R^lb are each independently methyl, ethyl, or propyl.

[0138] As generally defined herein, R is hydrogen, optionally substituted d_6 alkyl, or a nitrogen protecting group. In some embodiments, R^N1 is hydrogen. In some embodiments, R^N1 is optionally substituted Ci_6 alkyl. In certain embodiments, R^N1 is methyl. In certain embodiments, R^N1 is ethyl. In certain embodiments, R^N1 is propyl. In certain embodiments, R^N1 is a nitrogen protecting group. In certain embodiments, R ¹ is acyl. In certain embodiments, R^N1 is acetyl. In certain embodiments, R^N1 is Bn, BOC, Cbz, Troc, Fmoc, trifluoroacetyl, triphenylmethyl, or Ts.

[0139] As generally defined herein, R^N2 is hydrogen, optionally substituted Ci_6 alkyl, or a nitrogen protecting group. In some embodiments, R^N2 is hydrogen. In some embodiments, R^N2 is optionally substituted Ci_6 alkyl. In certain embodiments, R^N2 is methyl. In certain embodiments, R^N2 is ethyl. In certain embodiments, R^N2 is propyl. In certain embodiments, R^N2 is a nitrogen protecting group. In certain embodiments, R ² is acyl. In certain embodiments, R^N2 is acetyl. In certain embodiments, R^N2 is Bn, BOC, Cbz, Troc, Fmoc, trifluoroacetyl, triphenylmethyl, or Ts.

[0140] In certain embodiments, R^N1 and R⁰¹ are taken with the intervening atoms to form an optionally substituted heterocyclic ring. In certain embodiments, R^N1 and R⁰¹ are taken with the intervening atoms to form an optionally substituted 5-membered heterocyclic ring. In certain embodiments, R^N1 and R⁰¹ are taken with the intervening atoms to form an optionally

substituted 5-membered heterocyclic ring of the formula

₅ wherein R^N2 is defiend herein. In certain embodiments, R and R are taken with the intervening atoms to form an

optionally substituted 5-membered heterocyclic ring of the formula

.

[0141] As generally defined herein, p is an integer of 1 to 8 inclusive. In certain embodiments, p is 1. In certain embodiments, p is 2. In certain embodiments, p is 3. In certain embodiments, p is 4. In certain embodiments, p is 5. In certain embodiments, p is 6. In certain embodiments, p is 7. In certain embodiments, p is 8.

[0142] In certain embodiments, the glycan conjugate is of Formula (I-b):

(I-b)

or a pharmaceutically acceptable salt thereof, wherein: each instance of q is an integer of 1 to 8, inclusive.

[0143] In certain embodiments, the glycan conjugate is of Formula (I-c)

(I-c)

wherein each instance of R^8a, R^8b, and R⁹ is independently hydrogen, halogen, or optionally substituted Ci_6 alkyl; and t is an integer of 1 to 8, inclusive.

[0144] In certain embodiments, the glycan conjugate is of Formula (I-d)

(I-d)

wherein each instance of R ^a is independently hydrogen, optionally substituted C1-5 alkyl, optionally substituted acyl, or a nitrogen protecting group; and i is an integer of 1 to 8, inclusive.

[0145] In certain embodiments, the glycan conjugate is of Formula (I-e)

wherein each instance of R^Na is independently hydrogen, optionally substituted C1-5 alkyl, optionally substituted acyl, or a nitrogen protecting group; each instance of q is an integer of 1 to 8, inclusive; and i is an integer of 1 to 8, inclusive.

[0146] In certain embodiments, the glycan conjugate is of Formula (I-f)

(I-f) wherein each instance of q is an integer of 1 to 8, inclusive.

[0147] As generally defined herein, R^8a is independently hydrogen, halogen, or optionally substituted Ci_6 alkyl. In certain embodiments, R^8a is hydrogen. In certain embodiments, R^8a is halogen. In certain embodiments, R^8a is F. In certain embodiments, R^8a is CI. In certain embodiments, R^8a is Br. In certain embodiments, R^8a is I. In certain embodiments, R^8a is optionally substituted Ci_6 alkyl. In certain embodiments, R^8a is optionally substituted Ci_6 alkyl. In certain embodiments, R^8a is unsubstituted Ci_6 alkyl. In certain embodiments, R^8a is methyl, ethyl, or propyl.

[0148] As generally defined herein, R⁸ is independently hydrogen, halogen, or optionally substituted Ci_6 alkyl. In certain embodiments, R^8b is hydrogen. In certain embodiments, R^8b is halogen. In certain embodiments, R^8b is F. In certain embodiments, R^8b is CI. In certain embodiments, R^8b is Br. In certain embodiments, R^8b is I. In certain embodiments, R^8b is optionally substituted Ci_6 alkyl. In certain embodiments, R^8b is optionally substituted Ci-6 alkyl. In certain embodiments, R^8b is unsubstituted Q-e alkyl. In certain embodiments, R⁸ is methyl, ethyl, or propyl.

[0149] As generally defined herein, R⁹ is independently hydrogen, halogen, or optionally substituted Ci_6 alkyl. In certain embodiments, R⁹ is hydrogen. In certain embodiments, R⁹ is halogen. In certain embodiments, R⁹ is F. In certain embodiments, R⁹ is CI. In certain embodiments, R⁹ is Br. In certain embodiments, R⁹ is I. In certain embodiments, R⁹ is optionally substituted Ci_6 alkyl. In certain embodiments, R⁹ is optionally substituted Ci_6 alkyl. In certain embodiments, R⁹ is unsubstituted Ci_6 alkyl. In certain embodiments, R⁹ is methyl, ethyl, or propyl.

[0150] As generally defined herein, q is an integer of 1 to 8 inclusive. In certain embodiments, q is 1. In certain embodiments, q is 2. In certain embodiments, q is 3. In certain embodiments, q is 4. In certain embodiments, q is 5. In certain embodiments, q is 6. In certain embodiments, q is 7. In certain embodiments, q is 8.

[0151] As generally defined herein, t is an integer of 1 to 8 inclusive. In certain embodiments, t is 1. In certain embodiments, t is 2. In certain embodiments, t is 3. In certain embodiments, t is 4. In certain embodiments, t is 5. In certain embodiments, t is 6. In certain embodiments, t is 7. In certain embodiments, t is 8.

[0152] In some embodiments, p is 1 , q is an integer of 1 to 8 inclusive, and t is an integer of 1 to 8 inclusive.In some embodiments, p is 2, q is an integer of 1 to 8 inclusive, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 3, q is an integer of 1 to 8 inclusive, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is an integer of 1 to 8 inclusive, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 5, q is an integer of 1 to 8 inclusive, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 6, q is an integer of 1 to 8 inclusive, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 7, q is an integer of 1 to 8 inclusive, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 8, q is an integer of 1 to 8 inclusive, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is 1, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is 2, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is 3, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is 4, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is 5, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is 6, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is 7, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is 8, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is 2, and t is an integer of 1 to 8 inclusive. In some embodiments, p is 4, q is 2, and t is 1. In some embodiments, p is 4, q is 2, and t is 3. In some embodiments, p is 4, q is 2, and t is 4. In some embodiments, p is 4, q is 2, and t is 5. In some embodiments, p is 4, q is 2, and t is 6. In some embodiments, p is 4, q is 2, and t is 7. In some embodiments, p is 4, q is 2, and t is 8.

[0153] As generally defined herein, w is an integer from 1 to 100. In certain embodiments, w is an integer from 1 to 90. In certain embodiments, w is an integer from 1 to 80. In certain embodiments, w is an integer from 1 to 70. In certain embodiments, w is an integer from 1 to 60. In certain embodiments, w is an integer from 1 to 50. In certain embodiments, w is an integer from 1 to 40. In certain embodiments, w is an integer from 1 to 30. In certain embodiments, w is an integer from 1 to 20. In certain embodiments, w is an integer from 1 to 10. In certain embodiments, w is an integer from 1 to 5. In certain embodiments, w is an integer from 10 to 100. In certain embodiments, w is an integer from 10 to 20. In certain embodiments, w is an integer from 10 to 30. In certain embodiments, w is an integer from 20 to 30. In certain embodiments, w is an integer from 20 to 40. In certain embodiments, w is an integer from 30 to 50. In certain embodiments, w is an integer from 40 to 60. In certain embodiments, w is an integer from 50 to 70. In certain embodiments, w is an integer from 60 to 80. In certain embodiments, w is an integer from 70 to 90. In certain embodiments, w is an integer from 80 to 100.

[0154] As generally defined herein, y is 0 or an integer from 1 to 100. . In certain embodiments, y is 0. In certain embodiments, y is an integer from 1 to 100. In certain embodiments, y is an integer from 1 to 90. In certain embodiments, y is an integer from 1 to 80. In certain

embodiments, y is an integer from 1 to 70. In certain embodiments, y is an integer from 1 to 60. In certain embodiments, y is an integer from 1 to 50. In certain embodiments, y is an integer from 1 to 40. In certain embodiments, y is an integer from 1 to 30. In certain embodiments, y is an integer from 1 to 20. In certain embodiments, y is an integer from 1 to 10. In certain embodiments, y is an integer from 10 to 100. In certain embodiments, y is an integer from 10 to 20. In certain embodiments, y is an integer from 10 to 30. In certain embodiments, y is an integer from 20 to 30. In certain embodiments, y is an integer from 20 to 40. In certain embodiments, y is an integer from 30 to 50. In certain embodiments, y is an integer from 40 to 60. In certain embodiments, y is an integer from 50 to 70. In certain embodiments, y is an integer from 60 to 80. In certain embodiments, y is an integer from 70 to 90. In certain embodiments, y is an integer from 80 to 100.

[0155] In certain embodiments, n is an integer of 1 to 100, inclusive. In certain embodiments, n is an integer of 1 to 80, inclusive. In certain embodiments, n is an integer of 1 to 60, inclusive. In certain embodiments, n is an integer of 1 to 40, inclusive. In certain embodiments, n is an integer of 1 to 20, inclusive. In certain embodiments, n is an integer of 1 to 10, inclusive. In certain embodiments, n is an integer of 1 to 8, inclusive. As generally defined herein, n is an integer of 1 to 8 inclusive. In certain embodiments, n is 1. In certain embodiments, n is 2. In certain embodiments, n is 3. In certain embodiments, n is 4. In certain embodiments, n is 5. In certain embodiments, n is 6. In certain embodiments, n is 7. In certain embodiments, n is 8.

1 2 3 4 5 7

[0156] In certain embodiments, each of R , R , R , R , R , R , and R is indepently a carbohydrate of Formula (s-1):

wherein: each of R , R^Wa, R^¾Z , R^hJa, R^h3b, R , R , R^h5a, and R^¾5b is independently hydrogen, optionally substituted alkyl, -OR^so, or -N(R^SN)2; each instance of R^so is independently hydrogen, optionally substituted alkyl, optionally substituted heterocyclyl, or an oxygen protecting group; and each instance of R^SNis independently hydrogen, optionally substituted alkyl, or a nitrogen protecting group; or optionally two R^SN are taken with the intervening atoms to form a heterocyclic ring.

[0157] As generally defined herein, each instance of R^S1 is independently hydrogen, optionally substituted alkyl, -OR^so, or -N(R^SN)2. In certain embodiments, R^S1 is hydrogen. In certain embodiments, R^S1 is optionally substituted alkyl. In certain embodiments, R^S1 is substituted Ci_6 alkyl. In certain embodiments, R is unsubstituted Ci_₆ alkyl. In certain embodiments, R is methyl, ethyl, propyl, butyl, pentyl, or hexyl. [0158] In certain embodiments, R is -OR , wherein R is independently hydrogen, optionally substituted alkyl, optionally substituted alkyl, optionally substituted heterocyclyl, or an oxygen protecting group. In certain embodiments, R^S1 is -OH. In certain embodiments, R^S1 is -OR , wherein R is optionally substituted alkyl. In certain embodiments, R is -O-methyl, -

01

O-ethyl, or -O-propyl. In certain embodiments, R is optionally substituted -O-alkyl-aryl. In certain embodiments, R is -O-Bn. In certain embodiments, R is -OR , wherein R is an oxygen protecting group. In certain embodiments, R is -OR , wherein R is acyl. In certain embodiments, R is -O-acetyl or-O-Bz. In certain embodiments, R is -OR , wherein R is

* * * * S 1 * so ■ so * optionally substituted heterocyclyl. In certain embodiments, R is -OR , wherein R is an oxygen protecting group. In certain embodiments, R is -OR , wherein R is PMB, Bn, TBS, or TMS.

[0159] In certain embodiments, R^S1 is -N(R^SN)₂. In some embodiments, R^S1 is -N(R^SN)₂, wherein each R^SN is the same. In some embodiments, R ¹ is -N(R^SN)2, wherein each R^SN is different. In certain embodiments, R is -NH₂. In certain embodiments, R is -NHR . In certain embodiments, R is -NHR , wherein R is optionally substituted Ci_6 alkyl. In certain

S 1 SN SN

embodiments, R is -NHR , wherein R is unsubstituted Ci_₆ alkyl. In certain embodiment, R^S1 is -NHR^SN, wherein R^SN is substituted Ci_₆ alkyl. In certain embodiments, R^S1 is -NH- benzyl. In certain embodiment, R^S1 is -NHR^SN, wherein R^SN is a nitrogen protecting group. In certain embodiment, R^S1 is -NHAc, -NHBoc, Troc, Bn, or Cbz. In certain embodiments, R^S1 is -N(R ^N)₂, wherein each R^SN is independently optionally substituted Ci_e alkyl. In certain embodiments, R is -N(R )₂, wherein each R is independently unsubstituted Ci_6 alkyl. In certain embodiments, R is -N(CH₃)R , wherein each R is independently optionally substituted Ci_₆ alkyl. In certain embodiments, R^S1 is -N(CH₃)R ^N, wherein each R^SN is independently unsubstituted Ci_6 alkyl. In certain embodiments, R^S1 is -N(CH₂CH₃)R^SN, wherein each R^SN is independently optionally substituted Ci_6 alkyl. In certain embodiments, R^S1 is -N(CH₂CH₃)R , wherein each R is independently unsubstituted Ci_6 alkyl. In certain embodiments, R is -N(R )₂, wherein each R is independently selected from the group consisting of methyl, ethyl, isopropyl, isobutyl, isoamyl, and benzyl. In some embodiments, R^S1 is -N(R^SN)₂, wherein two R^SN groups are taken together with the intervening atoms to form an optionally substituted heterocyclic ring. For example, in certain embodiments, R ¹ is of the formula:

wherein R^sq is as defined herein, and sn is 0, 1, 2, or 3. [0160] As generally defined above, each instance of R ^a and R is independently hydrogen, optionally substituted alkyl, -OR^so, or -N(R^SN)2. In certain embodiments, at least one instance of R and R is hydrogen. In certain embodiments, at least one instance of R and R is optionally substituted alkyl. In certain embodiments, at least one instance of R^S2a and R^S2b is substituted Ci_₆ alkyl. In certain embodiments, at least one instance of R ^2a and R^S2 is unsubstituted Ci_e alkyl. In certain embodiments, at least one instance of R^S2a and R^S2b is methyl, ethyl, propyl, butyl, pentyl, or hexyl. In certain embodiments, at least one instance of R^S2a and R^S2b is isopropyl, isobutyl, or isoamyl. In certain embodiments, at least one instance of R^S2a and R^S2b is isobutyl. In certain embodiments, at least one instance of R^S2a and R^S2b is ierf-butyl. In certain embodiments, at least one instance of R^S2a and R^S2b is -OR^so, wherein R^so is

independently hydrogen, optionally substituted alkyl, optionally substituted alkyl, optionally substituted heterocyclyl, or an oxygen protecting group. In certain embodiments, at least one instance of R and R is -OH. In certain embodiments, at least one instance of R and R is -OR , wherein R is optionally substituted alkyl. In certain embodiments, at least one instance of R^S2a and R^S2b is -O-methyl, -O-ethyl, or -O-propyl. In certain embodiments, at least one instance of R^S2a and R^S2b is optionally substituted -O-alkyl-aryl. In certain

embodiments, at least one instance of R^S2a and R^S2b is -O-Bz. In certain embodiments, at least one instance of R and R is -OR , wherein R is an oxygen protecting group. In certain embodiments, at least one instance of R^S2a and R^S2b is -OR^so, wherein R^so is carbonyl. In certain embodiments, at least one instance of R and R is -OR , wherein R is acetyl. In

S2a S2b SO SO

certain embodiments, at least one instance of R and R is -OR , wherein R is optionally substituted heterocyclyl.

[0161] In certain embodiments, at least one instance of R ^2a and R^S2b is -N(R^SN)₂. In some embodiments, at least one instance of R^S2a and R^S2b is -N(R^SN)2, wherein each R^SN is the same. In some embodiments, at least one instance of R^S2a and R^S2b is -N(R^SN)2, wherein each R^SN is different. In certain embodiments, at least one instance of R^S2a and R^S2b is -NH₂. In certain embodiments, at least one instance of R^S2a and R^S2b is -NHR^SN. In certain embodiments, at least one instance of R^S2a and R^S2b is -NHR^SN, wherein R^SN is optionally substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S2a and R^S2b is -NHR^SN, wherein R^SN is unsubstituted Ci_6 alkyl. In certain embodiment, at least one instance of R^S2a and R ^2b is - NHR , wherein R is substituted Ci_6 alkyl. In certain embodiments, at least one instance of

is -

S ^" SN

NHR , wherein R is a nitrogen protecting group. In certain embodiment, at least one instance of R and R is -NHFmoc. In certain embodiment, at least one instance of R and R is - NHBoc. In certain embodiments, at least one instance of R^S2a and R^S2b is -N(R^SN)2, wherein each R is independently optionally substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S2a and R^S2b is -N(R^SN)2, wherein each R^SN is independently unsubstituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S2a and R^S2b is -N(CI¾)R^SN, wherein each R^SN is independently optionally substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S2a and R^S2b is N(CH₃)R^SN, wherein each R^SN is independently unsubstituted Ci_₆ alkyl. In certain embodiments, at least one instance of R^S2a and R^S2b is -N(CH₂CH )R^SN, wherein each R^SN is independently optionally substituted Ci_e alkyl. In certain embodiments, at least one instance of R^S2a and R^S2b is N(CH₂CH₃)R^SN, wherein each R^SN is independently unsubstituted Ci_6 alkyl. In certain embodiments, at least one instance of R^{S a} and R^S2b is - N(R )₂, wherein each R is independently selected from the group consisting of methyl, ethyl, isopropyl, isobutyl, isoamyl, and benzyl. In some embodiments, at least one instance of R ^2a and R is -N(R )₂, wherein two R groups are taken together with the intervening atoms to form an optionally substituted heterocyclic ring. For example, in certain embodiments, at least one instance of R^S2a and R^S2b is of the formula:

wherein R^sq is as defined herein, and sn is 0, 1, 2, or 3.

[0162] As generally defined above, each instance of RS3a and RS3b is independently hydrogen, optionally substituted alkyl, -ORSO, or -N(RSN)2. In certain embodiments, at least one instance of RS3a and RS3b is hydrogen. In certain embodiments, at least one instance of RS3a and RS3b is optionally substituted alkyl. In certain embodiments, at least one instance of RS3a and RS3b is substituted CI— 6 alkyl. In certain embodiments, at least one instance of RS3a and RS3b is unsubstituted Cl-6 alkyl. In certain embodiments, at least one instance of RS3a and RS3b is methyl, ethyl, propyl, butyl, pentyl, or hexyl. In certain embodiments, at least one instance of RS3a and RS3b is isopropyl, isobutyl, or isoamyl. In certain embodiments, at least one instance of RS3a and RS3b is isobutyl. In certain embodiments, at least one instance of RS3a and RS3b is tert-butyl. In certain embodiments, at least one instance of RS3a and RS3b is -ORSO, wherein RSO is independently hydrogen, optionally substituted alkyl, optionally substituted alkyl, optionally substituted heterocyclyl, or an oxygen protecting group. In certain embodiments, at least one instance of RS3a and RS3b is -OH. In certain embodiments, at least one instance of RS3a and RS3b is -ORSO, wherein RSO is optionally substituted alkyl. In certain embodiments, at least one instance of RS3a and RS3b is—O— methyl, -O-ethyl, or -O— propyl. In certain embodiments, at least one instance of RS3a and RS3b is optionally substituted -O-alkyl-aryl. In certain embodiments, at least one instance of RS3a and RS3b -O-Bz. In certain embodiments, at least one instance of RS3a and RS3b is -ORSO, wherein RSO is an oxygen protecting group. In certain embodiments, at least one instance of RS3a and RS3b is - ORSO, wherein RSO is carbonyl. In certain embodiments, at least one instance of RS3a and RS3b is -ORSO, wherein RSO is acetyl. In certain embodiments, at least one instance of RS3a and RS3b is -ORSO, wherein RSO is optionally substituted heterocyclyl. In certain

embodiments, at least one instance of RS3a and RS3b is -N(RSN)2. In some embodiments, at least one instance of RS3a and RS3b is -N(RSN)2, wherein each RSN is the same. In some embodiments, at least one instance of RS3a and RS3b is -N(RSN)2, wherein each RSN is different. In certain embodiments, at least one instance of RS3a and RS3b is -NH2. In certain embodiments, at least one instance of RS3a and RS3b is -NHRSN. In certain embodiments, at least one instance of RS3a and RS3b is -NHRSN, wherein RSN is optionally substituted Cl-6 alkyl. In certain embodiments, at least one instance of RS3a and RS3b is -NHRSN, wherein RSN is unsubstituted Cl-6 alkyl. In certain embodiment, at least one instance of RS3a and RS3b is -NHRSN, wherein RSN is substituted Cl-6 alkyl. In certain embodiments, at least one instance of RS3a and RS3b is -NH-benzyl. In certain embodiment, at least one instance of RS3a and RS3b is -NHRSN, wherein RSN is a nitrogen protecting group. In certain embodiment, at least one instance of RS3a and RS3b is -NHFmoc. In certain embodiment, at least one instance of RS3a and RS3b is -NHBoc. In certain embodiments, at least one instance of RS3a and RS3b is -N(RSN)2, wherein each RSN is independently optionally substituted Cl-6 alkyl. In certain embodiments, at least one instance of RS3a and RS3b is -N(RSN)2, wherein each RSN is independently unsubstituted Cl-6 alkyl. In certain embodiments, at least one instance of RS4a and RS4b is -N(CH3)RSN, wherein each RSN is independently optionally substituted Cl-6 alkyl. In certain embodiments, at least one instance of RS3a and RS3b is -N(CH3)RSN, wherein each RSN is independently unsubstituted Cl-6 alkyl. In certain embodiments, at least one instance of RS3a and RS3b is -N(CH2CH3)RSN, wherein each RSN is independently optionally substituted Cl-6 alkyl. In certain embodiments, at least one instance of RS3a and RS3b is -N(CH2CH3)RSN, wherein each RSN is independently unsubstituted Cl-6 alkyl. In certain embodiments, at least one instance of RS3a and RS3b is— N(RSN)2, wherein each RSN is independently selected from the group consisting of methyl, ethyl, isopropyl, isobutyl, isoamyl, and benzyl. In some embodiments, at least one instance of RS3a and RS3b is - N(RSN)2, wherein two RSN groups are taken together with the intervening atoms to form an optionally substituted heterocyclic ring. For example, in certain embodiments, at least one instance of R^{S a} and R^S3b is of the formula:

wherein R^sq is as defined herein, and sn is 0, 1, 2, or 3.

[0163] As generally defined above, each instance of R^S4a and R^{S b} is independently hydrogen, optionally substituted alkyl, -OR^so, or -N(R^SN)2. In certain embodiments, at least one instance of R^S4a and R^S4b is hydrogen. In certain embodiments, at least one instance of R^S4a and R^S4b is optionally substituted alkyl. In certain embodiments, at least one instance of R^S4a and R^S4b is substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S4a and R^S4 is unsubstituted Ci_e alkyl. In certain embodiments, at least one instance of R^{S a} and R^S4b is methyl, ethyl, propyl, butyl, pentyl, or hexyl. In certain embodiments, at least one instance of R^{S a} and R^S4b is isopropyl, isobutyl, or isoamyl. In certain embodiments, at least one instance of R^S4a and R^S4b is isobutyl. In certain embodiments, at least one instance of R^S4a and R^S4b is ierf-butyl.

[0164] In certain embodiments, at least one instance of R^S4a and R^S4b is -OR^so, wherein R^so is independently hydrogen, optionally substituted alkyl, optionally substituted alkyl, optionally substituted heterocyclyl, or an oxygen protecting group. In certain embodiments, at least one instance of R^S4a and R^{S b} is -OH. In certain embodiments, at least one instance of R^S4a and R^{S b} is -OR , wherein R is optionally substituted alkyl. In certain embodiments, at least one instance of R^S4a and R^S4b is -O-methyl, -O-ethyl, or -O-propyl. In certain embodiments, at least one instance of R^S4a and R^S4b is optionally substituted -O-alkyl-aryl. In certain

embodiments, at least one instance of R^S4a and R^S4b is -O-Bz. In certain embodiments, at least one instance of R^S4a and R^{S b} is -OR^so, wherein R^so is an oxygen protecting group. In certain embodiments, at least one instance of R^S4a and R^S4b is -OR^so, wherein R ^o is carbonyl. In certain embodiments, at least one instance of R^S4a and R^S4b is -OR^so, wherein R^so is acetyl. In certain embodiments, at least one instance of R^S4a and R^S4b is -OR^so, wherein R^so is optionally substituted heterocyclyl.

[0165] In certain embodiments, at least one instance of R ^4a and R ^4b is -N(R^SN)₂. In some embodiments, at least one instance of R^S4a and R^S4b is -N(R^SN)2, wherein each R^SN is the same. In some embodiments, at least one instance of R^S4a and R^S4b is -N(R^SN)2, wherein each R^SN is different. In certain embodiments, at least one instance of R^S4a and R^S4b is - ¾. In certain embodiments, at least one instance of R^S4a and R^S4b is -NHR^SN. In certain embodiments, at least one instance of R^S4a and R^{S b} is -NHR^SN, wherein R^SN is optionally substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S4a and R^S4b is -NHR^SN, wherein R^SN is unsubstituted Ci_6 alkyl. In certain embodiment, at least one instance of R^S4a and R ^4b is - NHR , wherein R is substituted Ci_6 alkyl. In certain embodiments, at least one instance of R ^a and R is -NH-benzyl. In certain embodiment, at least one instance of R ^a and R is - NHR , wherein R is a nitrogen protecting group. In certain embodiment, at least one instance of R^S4a and R^S4b is -NHFmoc. In certain embodiment, at least one instance of R^S4a and R^S4b is - NHBoc. In certain embodiments, at least one instance of R^S4a and R^S4b is -N(R^SN)2, wherein each R^SN is independently optionally substituted Ci_₆ alkyl. In certain embodiments, at least one instance of R^S4a and R^S4b is -N(R^SN)2, wherein each R^SN is independently unsubstituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S4a and R^S4b is -N(CH₃)R^S , wherein each R^SN is independently optionally substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S4a and R^S4b is -N(CH₃)R^SN, wherein each R^SN is independently unsubstituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S4a and R^S4b is -N(CH₂CH₃)R^SN, wherein each R^SN is independently optionally substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S4a and R^{S b} is -N(CH₂CH )R^SN, wherein each R^SN is independently unsubstituted Ci_e alkyl. In certain embodiments, at least one instance of R^S4a and R^S4b is - N(R^SN)2, wherein each R ^N is independently selected from the group consisting of methyl, ethyl, isopropyl, isobutyl, isoamyl, and benzyl.

[0166] In some embodiments, at least one instance of R^{S a} and R^S4b is -N(R^SN)₂, wherein two

_RSN groups are taken together with the intervening atoms to form an optionally substituted heterocyclic ring. For example, in certain embodiments, at least one instance of R^S4a and R^S4b is of the formula:

wherein R^sq is as defined herein, and sn is 0, 1, 2, or 3.

[0167] As generally defined above, each instance of R^S5a and R^S5b is independently hydrogen, optionally substituted alkyl, -OR^so, or -N(R^SN)2. In certain embodiments, at least one instance of R^S5a and R^S5b is hydrogen. In certain embodiments, at least one instance of R^S5a and R^S5b is optionally substituted alkyl. In certain embodiments, at least one instance of R^S5a and R^S5b is substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S5a and R^S5b is unsubstituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S5a and R^S5b is methyl, ethyl, propyl, butyl, pentyl, or hexyl. In certain embodiments, at least one instance of R^S5a and R^S5b is isopropyl, isobutyl, or isoamyl. In certain embodiments, at least one instance of R^S5a and R^S5b is isobutyl. In certain embodiments, at least one instance of R^S3a and R^S5b is ieri-butyl.

[0168] In certain embodiments, at least one instance of R^S5a and R^S5b is -OR^so, wherein R^so is independently hydrogen, optionally substituted alkyl, optionally substituted alkyl, optionally substituted heterocyclyl, or an oxygen protecting group. In certain embodiments, at least one instance of R ^a and R is -OH. In certain embodiments, at least one instance of R ^a and R is -OR , wherein R is optionally substituted alkyl. In certain embodiments, at least one instance of R^S5a and R^S5b is -O-methyl, -O-ethyl, or -O-propyl. In certain embodiments, at least one instance of R^S5a and R^S5b is optionally substituted -O-alkyl-aryl. In certain embodiments, at least one instance of R^S5a and R^S5b is -O-Bz. In certain embodiments, at least one instance of R^S5a and R^S5b is -OR^so, wherein R^so is an oxygen protecting group. In certain embodiments, at least one instance of R^S5a and R^S5b is -OR^so, wherein R^so is carbonyl. In certain embodiments, at least one instance of R^S5a and R^S5b is -OR^so, wherein R^so is acetyl. In certain embodiments, at least one instance of R^S5a and R^S5b is -OR^so, wherein R^so is optionally substituted heterocyclyl.

[0169] In certain embodiments, at least one instance of R^S5a and R^S5b is -N(R^SN)₂. In some embodiments, at least one instance of R^S5a and R^S5b is -N(R^SN)2, wherein each R^SN is the same. In some embodiments, at least one instance of R^S5a and R^S5b is -N(R^S )2, wherein each R^SN is different. In certain embodiments, at least one instance of R^S5a and R^S5b is -NH₂. In certain embodiments, at least one instance of R^S5a and R^S5b is -NHR^SN. In certain embodiments, at least one instance of R^S5a and R^{S b} is -NHR^S , wherein R^SN is optionally substituted alkyl. In certain embodiments, at least one instance of R^S5a and R^S5b is -NHR^SN, wherein R^SN is unsubstituted Ci_6 alkyl. In certain embodiment, at least one instance R^S5a and R^S5b is -NHR^SN, wherein R^SN is substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S5a and R^S5b is -NH-benzyl. In certain embodiment, at least one instance of R^S5a and R^S5b is -NHR^SN, wherein R^SN is a nitrogen protecting group. In certain embodiment, at least one instance of R^S4a and R^S4b is -NHFmoc. In certain embodiment, at least one instance of R^S5a and R^S5b is -NHBoc.

[0170] In certain embodiments, at least one instance of R ^5a and R^S5b is -N(R^SN)₂, wherein each R^SN is independently optionally substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S5a and R^S5b is -N(R^SN)2, wherein each R^SN is independently unsubstituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S5a and R^S5b is -N(CH₃)R^SN, wherein each R^SN is independently optionally substituted Ci_6 alkyl. In certain embodiments, at least one instance of R^S5a and R^S3b is -N(CH₃)R^SN, wherein each R^SN is independently unsubstituted Ci_₆ alkyl. In certain embodiments, at least one instance of R^S5a and R^S5b is -N(CH₂CH₃)R^SN, wherein each R^SN is independently optionally substituted Ci_6 alkyl. In certain embodiments, at least one instance of R ^5a and R^S5b is -N(CH₂CH₃)R^SN, wherein each R^SN is independently unsubstituted Ci_e alkyl. In certain embodiments, at least one instance of R^S5a and R^S5b is - N(R^8N)₂, wherein each R^SN is independently selected from the group consisting of methyl, ethyl, isopropyl, isobutyl, isoamyl, and benzyl. [0171] In some embodiments, at least one instance of R ^a and R is -N(R )₂, wherein two

R groups are taken together with the intervening atoms to form an optionally substituted heterocyclic ring. For example, in certain embodiments, at least one instance of R^S5 and R^S5b is of the formula:

wherein R^sq is as defined herein, and sn is 0, 1, 2, or 3.

[0172] As used herein, each instance R^sq is independently halogen, optionally substituted alkyl, -OR^S01, or -N(R^SN1)₂, wherein R^S01 is independently hydrogen, optionally substituted alkyl, or an oxygen protecting group; and R^SN1 is independently hydrogen, optionally substituted alkyl, or a nitrogen protecting group; or optionally two R^SN1 are taken together with the intervening atoms to form an optionally substituted heterocyclic ring.

[0173] As generally defined herein, each instance of R^so is independently hydrogen, optionally substituted alkyl, carbonyl, optionally substituted heterocyclyl, or an oxygen protecting group.

[0174] In certain embodiments, R^so is hydrogen. In certain embodiments, R^so is optionally substituted alkyl. In certain embodiments, R^so is optionally substituted Ci_6 alkyl. In certain embodiments, R is methyl, ethyl, or propyl. In certain embodiments, R is optionally substituted aralkyl, e.g., optionally substituted benzyl (Bn). In certain embodiments, R^so is optionally substituted heterocyclyl. In certain embodiments, R^so is carbonyl. In certain embodiments, R^so is -C(=0)CH₃ (acetyl, Ac). In certain embodiments, R^so is -C(=0)Ph (benzoyl, Bz). In certain embodiments, R^so is an oxygen protecting group.

[0175] As generally defined herein, each instance of R^SN is independently hydrogen, optionally substituted alkyl, optionally substituted heterocyclyl, or a nitrogen protecting group; or optionally two R^SN are taken together with the intervening atoms to form an optionally substituted heterocyclic ring. In certain embodiments, R^SN is hydrogen. In certain embodiments, R^SN is optionally substituted alkyl. In certain embodiments, R^SN is optionally substituted alkyl. In certain embodiments, R^SN is methyl, ethyl, or propyl. In certain embodiments, R^SN is substituted aralkyl, e.g., optionally substituted benzyl (Bn). In certain embodiments, R^SN is optionally substituted heterocyclyl. In certain embodiments, R^SN is carbonyl. In certain embodiments, R^SNis carbonyl. In certain embodiments, R^SN is -C(=0)CH3 (acetyl, Ac). In certain embodiments, R^SN is -C(=0)Ph (benzoyl, Bz). In certain embodiments, R^S is a nitrogen protecting group.

[0176] As generally defined herein, each instance of L¹ is independently a bond, -0-, -S-, - NR^Lla— , -C(=0)-, -NR^LlaC(=0)-, -NR^LlaC(=0)0- -C(=0)NR^Lla- -OC(=0)NR^Lla- - SC(=0)-, -C(=0)S- -OC(=0)-, -C(=0)0-, -NR^LlaC(=S)-, -C(=S)NR^Lla- trans- CR^Llb=CR^Llb-, czs-CR^Llb=CR^Llb- -C≡C-, -OC(R^Llb)₂-, -C(R^Llb)₂0-, -NR^LlaC(R^Llb)₂-, - C(R^Llb)₂NR^Lla-, -SC(R^L1V, -C(R^Llb)₂S-, S(=0)₂0 , OS(=0)₂ , -S(=0)₂NR^Lla-, - NR^LlaS(=0)₂-, or an optionally substituted Ci_₂o hydrocarbon chain, optionally wherein one or more carbon units of the hydrocarbon chain is repL laced with -0-, -S-, -NR^Lla-, -C(=0)-, NR^LlaC(=0)_

-OC(=0)NR^Lla-, -SC(=0)-, -C(=0)S-, - OC(=0)-, -C(=0)0-, -NR^LlaC(=S)-, -C(=S)NR^Lla-, irara-CR^Llb=CR^Llb-, ds-CR^Llb=CR^Llb-, -C≡C-, -S(=0)₂0- -OS(=0)₂- -S(=0)₂NR^Lla- or -NR^LlaS(=0)₂- wherein R^Lla is hydrogen, optionally substituted Ci__e alkyl, or a nitrogen protecting group, or R^Lla is joined with the adjacent carbon atom to form an optionally substituted heterocyclic ring, and wherein each occurrence of R^Llb is independently selected from the group consisting of hydrogen, halogen, optionally substituted Ci-io alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl, or R^Llb is joined with the adjacent carbon or nitrogen or oxygen atom to form an optionally substituted carbocyclic or heterocyclic ring, or two R^Llb groups are joined to form an optionally substituted carbocyclic or optionally substituted heterocyclic ring. In certain embodiments, L¹ is a bond. In certain embodiments, L¹ is an optionally substituted Ci_₂o hydrocarbon chain, optionally wherein one or more carbon units of the hydrocarbon chain is repLlaced with -0-, -S-, -NR^L1 -, -C(=0)-, NR^LlaC(=0)-, - NR^LlaC(=0)0- -C(=0)NR^L1 -OC(=0)NR^Lla- -SC(=0)-, -C(=0)S- -OC(=0)-, - C(=0)0- -NR^LlaC(=S)-, -C(=S)NR^Lla- ira«s-CR^Llb=CR^Llb-, czs-CR^Llb=CR^Llb- -OC-, - S(=0)₂0- -OS(=0)₂- -S(=0)₂NR^Lla- or -NR^LlaS(=0)₂-. In certain embodiments, L¹ is of the formula -C(=0)-(CH₂)_q-S- or -C(=0)-(CH₂)_q-C(=0)-, wherein q is an integer of 1 to 8, inclusive.

[0177] In certain embodiments, q is 1. In certain embodiments, q is 2. In certain embodiments, q is 3. In certain embodiments, q is 4. In certain embodiments, q is 5. In certain embodiments, q is 6. In certain embodiments, q is 7. In certain embodiments, q is 8.

[0178] In certain embodiments, R^Lla is hydrogen or optionally substituted Ci_6 alkyl. In certain embodiments, R^Lla is hydrogen. In certain embodiments, R^Lla is methyl, ethyl, or propyl.

[0179] In certain embodiments, R^Llb is hydrogen or optionally substituted Ci_6 alkyl. In certain embodiments, R^LIb is hydrogen. In certain embodiments, R^Llb is methyl, ethyl, or propyl.

[0180] As generally defined herein, each instance of L² is a moiety derived from a crosslinking reagent capable of crosslinking the carrier and L^!-H. Crosslinking reagents suited to the invention are widely known in the art (see, for example, 1994 Pierce Technical Handbook: cross-linking available at http://www.piercenet.com/resources/browse.cfm?fldID=184), including bromoacetic NHS ester, 6-(iodoacetamido)caproic acid NHS ester, maleimidoacetic acid NHS ester, maleimidobenzoic acide NHS ester, etc. In certain embodiments, each instance of L² is a moiety derived from a crosslinking reagent capable of crosslinking the amino group on embodiments, L² is of the formula

, or wherein R^8a, R^8b and R⁹ are each independently hydrogen, halogen, or optionally substituted Ci_6 alkyl; R^Na is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group; i is an integer from 1 to 8, inclusive; and j is an integer from 1 to 8, inclusive, end a is linked to L^L-H; and end b is linked carrier. In certain

embodiments, L is of the formula

diments, L is of the a, R^8b and R⁹ are hydrogen. In certain embodiments,

wherein R^8a, R^8b and R⁹ are hydrogen and t is 5.

[0181] As generally defined herein, each instance of R^CN is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group. In some embodiments, R^CN is hydrogen. In some embodiments, R^CN is optionally substituted Ci_6 alkyl. In certain embodiments, R is methyl. In certain embodiments, R is ethyl. In certain embodiments, R is propyl. In certain embodiments, R is optionally substituted acyl. In certain embodiments, R^CN is acetyl. In certain embodiments, R^CN is a nitrogen protecting group. In certain embodiments, R^N2 is Bn, BOC, Cbz, Troc, Fmoc, trifluoroacetyl, triphenylmethyl, or Ts.

[0182] As generally defined herein, each instance of R^BN is independently hydrogen, optionally substituted Ci_₆ alkyl, optionally substituted acyl, or a nitrogen protecting group. In some embodiments, R^BN is hydrogen. In some embodiments, R^BN is optionally substituted Ci_6 alkyl. In certain embodiments, R^BN is methyl. In certain embodiments, R^BN is ethyl. In certain embodiments, R^BN is propyl. In certain embodiments, R^BN is optionally substituted acyl. In certain embodiments, is acetyl. In certain embodiments, R is a nitrogen protecting group. In certain embodiments, R^N2 is Bn, BOC, Cbz, Troc, Fmoc, trifluoroacetyl, triphenylmethyl, or Ts.

[0183] As generally defined herein, L^3C is independently a crosslinking reagent or a crosslining reagent moiety whereint the crosslinking reagent is capable of crosslinking the carrier and Ι Η. In certain

wherein R , R , and R ^ca are each independently hydrogen, halogen, or optionally substituted Ci_₆ alkyl; each of R^P3 and R^P4 independently optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted 3- to 6- membered heterocyclyl, optionally substituted aryl, optionally substituted heteroaryl, - OR^3CO, -SR ^CS, or -N(R^3CN)₂; R ^C0 is independently hydrogen, substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted 3- to 6- membered heterocyclyl, optionally substituted aryl, optionally substituted heteroaryl, or an oxygen protecting group; R^2CS is independently hydrogen, substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted 3- to 6- membered heterocyclyl, optionally substituted aryl, optionally substituted heteroaryl, or a sulfur protecting group; each instance of R^3CN is independently hydrogen, substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted 3- to 6- membered heterocyclyl, optionally substituted aryl, optionally substituted heteroaryl, or a nitrogen protecting group.

[0184] In certain embodiments, R^P1 is hydrogen. In certain embodiments, R^P1 is halogen. In certain embodiments, R^P1 is optionally substituted Ci_6 alkyl. In certain embodiments, R^P1 is methyl, ethyl, or propyl.

[0185] In certain embodiments, R^P2 is hydrogen. In certain embodiments, R^P2 is halogen. In certain embodiments, R^P2 is optionally substituted Ci_6 alkyl. In certain embodiments, R^P2 is methyl, ethyl, or propyl.

[0186] In certain embodiments, R^P3 is hydrogen. In certain embodiments, R^P3 is halogen. In certain embodiments, R^P3 is optionally substituted Ci_6 alkyl. In certain embodiments, R^P3 is methyl, ethyl, or propyl. In certai ^■n embodi *ments, R P3 i *s -SR 3CS , wherei *n R 30S i *s opti *onally substituted alkyl. [0187] In certain embodiments, R^P4 is hydrogen. In certain embodiments, R^P4 is halogen. In certain embodiments, R^P4 is optionally substituted Ci_6 alkyl. In certain embodiments, R^P4 is

P4 3 S 3CS

methyl, ethyl, or propyl. In certain embodiments, R is -SR , wherein R is optionally substituted alkyl.

[0188] In certain embodiments, R^3Ca is hydrogen. In certain embodiments, R^3Ca is halogen. In certain embodiments, R ^C is optionally substituted Ci_6 alkyl. In certain embodiments, R^3Ca is methyl, ethyl, or propyl.

[0189] In c , ^3C is one of the following formulae:

diments, L^3C is a crosslinking reagent of the formula

In certain embodiments, L is a crosslinking reagent moiety of the

formula

prepared from a nucleophilic reaction with the crosslinking

reagent of the formula is a crosslinking

reagent moiety of the fo

rmula wherein R^P3 is hydrogen and R^P4 is -SR^3CS; or R^P3 is -SR^3CS and R^P4 is hydrogen.

[0191] In some embodiments, the glycan moiety is of Formula (II)

(II)

or a pharmaceutically acceptable salt thereof.

[0192] In

(in)

or a pharmaceutically acceptable salt thereof.

[0193] In certain embodiments, R¹ and R² are hydrogen. In certain embodiments, R¹, R², and R⁶ are all hydrogen. In certain embodiments, R¹, R², and R³ are all hydrogen. In certain embodiments, R¹ and R² are acetyl. In certain embodiments, R¹, R², and R³ are acetyl. In certain embodiments, R¹, R², and R⁶ are acetyl. In certain embodiments, R¹ and R² are Bn. In certain embodiments, R¹ is TBDPS and R² and R³ are taken with the intervening atoms to form a 5-

membered heterocyclic ring of the formula

, wherein R^ra and R^rb are each independently methyl, ethyl, or propyl.

[0194] In certain embodiments, R⁵ and R⁶ are hydrogen. In certain embodiments, R⁵, R⁶ and R⁷ are hydrogen. In certain embodiments, R⁵, R⁶ and R⁷ are acetyl. In certain embodiments, R⁵, R⁶ and R⁷ are acetyl. In certain embodiments, R⁵, R⁶ and R⁷ are Bn.

[0195] In certain embodiments, R^N1 is acetyl and R N¹ 2 i^■s hydrogen. In certain embodiments, R jN^J l and R are hydrog [0196] Any of the glycan moieties described herein may be conjugated with a carrier to enhance the immunogenicity of the glycan moieties. Such carriers include, but are not limited to, a protein, a lipid, a lipolized protein, a virus, a peptide comprising a T cell epitope, or a dendrimer of glycopeptides. In some embodiments, the carrier is a toxin protein selected from the group consisting of diphtheria toxin cross-reacting material 1 7 (DT-CRM 1 7), diphtheria toxoid, tetanus toxoid, and outer-membrane protein (OMP). In other examples, the carrier is the toxin protein is DT-CRM 197.

[0198] In another aspect, the present invention provides a glycan conjugate mixture comprising at least two of the glycan conjugates described herein. In some embodiments, the average value of w (average molar ratio of the glycan moiety to the carrier) in the glycan conjugate mixture provided herein is from about 1.0 to about 100.0, e.g., about 1.0 to about 90.0; about 1.0 to about 80.0; about 1.0 to about 70.0; about 1.0 to about 60.0; about 1.0 to about 50.0; about 1.0 to about 40.0; about l .O to about 30.; about l .O to about 20.0; ; or about l .O to about 5.0. In certain embodiments, the glycan conjugate mixture has an average value of w of 5.7, 4.9, 2.9, 2.8, or 3.1 , In certain embodiments, the glycan conjugate mixture has an average value of w of 4.9, 2.9, 2.8, or 3.1.

[0199] In some embodiments, for all the compounds and glycan conjugates described herein, all the variants in each unit of the n bracket can be the same, completely different, or partially different. In some embodiments, all the variants in each unit of the n bracket are the same. In some embodiments, all the variants in each unit of the n bracket are completely different. In some embodiments, some of the variants in each unit of the n bracket are partially different. For example, when n is 3, the compound of Formula (F-l) is of the formula

unit C

wherein unit A, unit B, unit C can be the same, completely different, or partially different (i.e. R¹, R², R³, R⁴, R⁵, R⁶, R⁷, R^N1, and R^N2 can be the same, completely different, or partially different in unit A, unit B, or unit C).

[0200] In some embodiments, the glycan conjugates described herein encompass hybrid conjugates to the carrier. In some embodiments, for all glycan conjugates described herein, all the variants in each unit of the w bracket can be the same, completely different, or partially different. In some embodiments, all the variants in each unit of the w bracket are the same. In some embodiments, all the variants in each unit of the w bracket are different. In some embodiments, some of the variants in each unit of the w bracket are partially different. For example, when w is 3, the compound of Formula (I-a) is of the structure in Figure 10, wherein branch D, branch E, and branch F can be the same, completely different, or partially different (i.e. R¹, R², R³, R⁴, R⁵, R⁶, R⁷, R^N1, R^N2, n, p, R^BN, R^CN, L¹, and L² can be the same, completely different, or partially different in branch D, branch E, branch F).

Method of Synthesizing Glycan Moieties and Glycan Conjugates

[0201] The present invention provides methods of preparing glycan moiety of Formula (I-a). The method of preparing the glycan conjugates may comprise coupling a compound of Formula

(C-l)

(C-l) or a salt thereof,

with a compound of the Formula (C-2)

(C-2)

wherein L^2C is a crosslinking reagent capable of crosslinking an amino group and ^l-H.

[0202] As generally defined herein, L^2C is independently a crosslinking reagent capable of crosslinking the carrier and L 1 -H. In certain embodiments, L 20 is a crosslinking reagent capable of crosslinking an amine group and -SH. In certain embodiments, L^2C is one of the following formulae:

or a salt thereof,

wherein each instance of R^P1 and R^P2are each independently hydrogen, halogen, or optionally substituted CI -6 alkyl; each instance of R^2Ca is a leaving group selected from selected from -Br, -CI, -I, -OS(=0)₂R ^CO, or -OS(=0)R ^CO, wherein R^2C0 is selected from the group consisting of optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl; and each of t and i is independently an integer of 1 to 8, inclusive.

[0203] As generally used herein, R^P1 and R^P2 are each independently hydrogen or optionally substituted Ci_6 alkyl. In certain embodiments, R^P1 is hydrogen. In certain embodiments, R^P1 is optionally substituted Ci_6 alkyl. In certain embodiments, R^P1 is unsubstituted Ci_6 alkyl. In certain embodiments, R^P1 is methyl, ethyl, or propyl. In certain embodiments, R^P2 is hydrogen. In certain embodiments, R^P2 is optionally substituted Ci_₆ alkyl. In certain embodiments, R^P2 is unsubstituted Ci_e alkyl. In certain embodiments, R^P2 is methyl, ethyl, or propyl. In certain embodiments, both R^P1 and R^P2 are hydrogen. In certain embodiments, R^P1 and R^P2 are each independently optionally substituted Ci_6 alkyl.

[0204] As generally used herein, R^2Ca is a leaving group. In certain embodiments, R^2Ca is a leaving group selected from selected from the group consisting of -Br, -CI, -I, -OS(=0)2R^2CO, or -OS(=0)R , wherein R is selected from the group consisting of optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl. In certain embodiments, R ^a is -Br, -CI, or -I. In certain embodiments, R^2Ca is -OS(=0)₂R^2CO, or -OS(=0)R^2CO, wherein R^2C0 is optionally substituted alkyl such as methyl, ethyl, or propyl.

[0205] In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 1 to about 100.

[0206] In certain embodiments, the coupling is carried out in the presence of phosphate buffered saline (PBS).

[0207] The synthesis of the glycan moiety of Formula (I) generally involves coupling a compound of Formula (C-1) with a compound of Formula (C-2). In certain embodiments, the coupling reaction is carried out in the presence of a buffer. In certain embodiments, the coupling reaction is carried out in pH from about 6.0 to 9.0. In certain embodiments, the coupling reaction is carried out under pH from about 6.0 to 8.0. In certain embodiments, the coupling reaction is carried out under pH from about 7.0 to 9.0. In certain embodiments, the coupling reaction is carried out under pH from about 7.5 to 8.0. In certain embodiments, the coupling reaction is carried out under pH from about 7.0 to 7.5. In certain embodiments, the coupling reaction is carried out in the presence of phosphate buffered saline (PBS).

[0208] In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 1 to about 100. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 1 to about 20. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 10 to about 30. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 20 to about 40. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 30 to about 50. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 40 to about 60. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 50 to about 70. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 60 to about 80. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 70 to about 90. In certain embodiments, the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 80 to about 100.

[0209] In certain embodiments, the method of preparing a glycan conjugate as decribed herein further comprising glycosylating a compound of Formula (C-3)

a compound of Formula (C-4)

(C-5)

wherein v is an integer of 1 to 99 inclusive; u is 0, 1 , 2, 3, 4, or 5; each occurrence of R^E is independently halogen, -CN, -NO₂, -N3, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted phenyl, optionally substituted heterocyclyl, optionally substituted heteroaryl, -OR^CE, -N(R^CE)₂, - SR^CE, -C(=0)R^CE, -C(=0)OR^CE, or -C(=0)N(R^CE)₂, wherein each R^CE is independently selected from the group consisting of hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl; or an oxygen protecting group when attached to an oxygen, or a nitrogen protecting group when attached to nitrogen, or a sulfur protecting group when attached to sulfur; and R ³ is independently hydrogen, optionally substituted alkyl, or a nitrogen protecting group.

[0210] As generally defined herein, v is an integer from 1 to 99 inclusive. In certain

embodiments, v is an integer from 1 to 90. In certain embodiments, v is an integer from 1 to 80. In certain embodiments, v is an integer from 1 to 70. In certain embodiments, v is an integer from 1 to 60. In certain embodiments, v is an integer from 1 to 50. In certain embodiments, v is an integer from 1 to 40. In certain embodiments, v is an integer from 1 to 30. In certain embodiments, v is an integer from 1 to 20. In certain embodiments, v is an integer from 1 to 10. In certain embodiments, v is an integer from 10 to 99. In certain embodiments, v is an integer from 10 to 20. In certain embodiments, v is an integer from 10 to 30. In certain embodiments, v is an integer from 20 to 30. In certain embodiments, v is an integer from 20 to 40. In certain embodiments, v is an integer from 30 to 50. In certain embodiments, v is an integer from 40 to 60. In certain embodiments, v is an integer from 50 to 70. In certain embodiments, v is an integer from 60 to 80. In certain embodiments, v is an integer from 70 to 90. In certain embodiments, v is an integer from 80 to 99.

[0211] As generally defined herein, u is 0, 1 , 2, 3, 4, or 5. In certain embodiments, u is 0. In certain embodiments, u is 1. In certain embodiments, u is 2. In certain embodiments, u is 3. In certain embodiments, u is 4. In certain embodiments, u is 5.

[0212] As generally defined herein, each occurrence of R^E is independently halogen, -CN, -N0₂, -N3, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted phenyl, optionally substituted heterocyclyl, optionally substituted heteroaryl, -OR^CE, -N(R^CE)₂, -SR^CE, -C(=0)R^CE, - C(=0)OR , or -C(=0)N(R )₂, wherein each R is independently selected from the group consisting of hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl; or an oxygen protecting group when attached to an oxygen, or a nitrogen protecting group when attached to nitrogen, or a sulfur protecting group when attached to sulfur. In certain embodiments, R^E is halogen. In certain embodiments, R^Eis F. In certain embodiments, R^Eis CI. In certain embodiments, R^Eis Br. In certain embodiments, R^Eis I. In certain embodiments, R^E is an optionally substituted alkyl. In certain embodiments, R^Eis an optionally substituted Ci_e alkyl. In certain embodiments, R^Eis an unsubstituted Ci_6 alkyl. In certain embodiments, R^E is methyl, ethyl, or propyl. [0213] In certain embodiments, u is 1 and R^E is an optionally substituted Ci_6 alkyl. In certain embodiments, u is 1 and R^Eis unsubstituted Ci_6 alkyl. In certain embodiments, u is 1 and R^Eis methyl, ethyl, or propyl.

[0214] As generally defined herein, R ³ is independently hydrogen, optionally substituted alkyl, acyl, or a nitrogen protecting group. In some embodiments, R^N3 is hydrogen. In some embodiments, R^N3 is optionally substituted Ci_6 alkyl. In certain embodiments, R^N3 is methyl. In certain embodiments, R^N3 is ethyl. In certain embodiments, R^N3 is propyl. In certain

embodiments, R^N3 is acetyl. In certain embodiments, R^N3 is a nitrogen protecting group. In certain embodiments, R^N3 is acyl. In certain embodiments, R^N3 is Bn, BOC, Cbz, Troc, Fmoc, trifluoroacetyl, triphenylmethyl, or Ts.

[0215] The glycosylation reaction is carried out in the present of MS/ TfOH, MS/AgOTf, NIS/TMSOTf, or MS/BF3-OEt₂. In certain embodiments, the glycosylation reaction is carried out in the present of MS/ TfOH.

[0216] In certain embodiments, the method of preparing a glycan conjugate described herein further comprises reacting the compound of Formula (C-5) in the presence of a base to give a compound of Formula (C-6)

(C-6)

[0217] In some embodiments, the base used in the reaction may be an inorganic base such as sodium hydroxide, potassium hydroxide, sodium carbonate, sodium hydrogencarbonate, potassium carbonate, potassium hydrogencarbonate and the like. In some embodiments, the base is NaOMe. In certain embodiemtns, the Zemplen condition is adopted including NaOMe in the presence of MeOH. It is to be understood the condition can be modified to other suitable base and organic solvent.

[0218] In certain embodiments, the method of preparing a glycan conjugate described herein further comprises reacting the compound of Formula (C-6) with a reducing agent to give a compound of Formula (C-7)

(C-7)

[0219] In certain embodiments, the reducing agent is any agent providing hydrogen to the compound in the reaction. In certain embodiments, the reducing agent is BF₃.OEt₂, H₂, organo silane with Si-H bond (e.g. (TMS^SiH or Et₃SiH). In certain embodiments, the reducing agent is BF₃.OEt₂.

[0220] A method of preparing a glycan conjugate described herein may comprise reacting (a) activating a compound of Formula (C-7) to give a compound of Formula (C-l); and (b) activating a carrier to give a compound of Formula (C-2).

[0221] In certain embodiments, the activating agent for the compound of Formula (C-7) is a

compound of Formula (C-8)

each of ql and q2 is indepdentnly an integer of 1 to 5, inclusive; and L is -S-. In certain embodiments, ql and q2 are 1. In certain embodiments, ql and q2 are 2. In certain embodiments, the activating reaction is carried out under pH from about 6.0 to 9.0. In certain embodiments, the activating reaction is carried out under pH from about 6.0 to 8.0. In certain embodiments, the activating reaction is carried out under pH from about 7.0 to 9.0. In certain embodiments, the activating reaction is carried out under pH from about 7.5 to 8.0. In certain embodiments, the activating reaction is carried out under pH from about 7.0 to 7.5. In certain embodiments, the activating reaction is carried out in the presence of a buffer. In certain embodiments, the activating reaction is carried out in the presence of phosphate buffered saline (PBS).

[0222] In certain embodiments, the activating agent for the carrier is a compound of Formula

(C-9)

(C-9), wherein t is as defined herein. In certain embodiments, t is 3. In certain embodiments, t is 4. In certain embodiments, t is 5. In certain embodiments, t is 6. In certain embodiments, t is 7. In certain embodiments, the activating reaction is carried out under pH from about 6.0 to 9.0. In certain embodiments, the activating reaction is carried out under pH from about 6.0 to 8.0. In certain embodiments, the activating reaction is carried out under pH from about 7.0 to 9.0. In certain embodiments, the activating reaction is carried out under pH from about 7.5 to 8.0. In certain embodiments, the activating reaction is carried out under pH from about 7.0 to 7.5. In certain embodiments, the activating reaction is carried out in the presence of a buffer. In certain embodiments, the activating reaction is carried out in the presence of phosphate buffered saline (PBS).

[0223] As used herein, an activating reagent denotes a reagent which can react with one of the starting materials of a chemical reaction to form one or more active intermediate which subsequently facilitates the completion of the reaction. The active intermediate may not be stable enough to be separated and characterized. Examples of the activating reagent include, but are not limited to the coupling reagents used in amide/peptide synthesis, such as carbodiimide compound (EDC, DCC, DIC, and the like) and benzotriazole compounds (such as HOBt and HOAt); certain oxides and chloride (such as P₂O₅ and POCI₃); a reagent which react with a molecule to form a leaving group (such as MsCl, TfzO, and reagents for Mitsunobu reaction); and etc. In certain embodiments, the activating reagent is 3,3'-Dithiobis(sulfosuccinimidyl propionate) (DTSSP) and/or N-fe-maleimidocaproyloxyjsulfosuccinimide ester) (sulfo-EMCS).

[0224] It is to be understood that a compound of Formula (F-l) can also be prepared following the general procedure of preparing a compound of Formulae (C-5), (C-6), and (C-3) as described above.

Immunogenic compositions

[0225] The present invention provides immunogenic compositions comprising a glycan conjugate drescribed herein and a pharmaceutically acceptable excipient. In certain

embodiments, the provided immunogenic composition further comprise an adjuvant. Such immunogenic compositions can be used to elicit desired immune responses, such as immune responses specific to the glycan conjugate, particularly the glycan moiety in the conjugate. In certain embodiments, a provided composition comprises two or more glycan conjugates described herein.

[0226] The immunogenic compositionsdescribed herein can be prepared by any method known in the art of pharmacology, for example, as described in U.S. Pat. Nos. 4,601,903; 4,599,231 ; 4,599,230; and 4,596,792, all of which are incorporated by reference herein. In general, such preparatory methods include the steps of bringing an the glycan conjugate described herein into association with an adjuvant and/or a pharmaceutically acceptable excipient and/or one or more other accessory ingredients, and then, if necessary and/or desirable, shaping and/or packaging the product into a desired single- or multi-dose unit. [0227] The immunogenic compositions disclosed herein can be included in a pharmaceutical or nutraceutical composition together with additional active agents, carriers, vehicles, excipients, or auxiliary agents identifiable by a person skilled in the art upon reading of the present disclosure.

[0228] The immunogenic compositions described herein may further comprise an adjuvant. An adjuvant is an agent that modifies the immunogenicity of the glycan conjugate in the composition. Adjuvant typically does not elicit immune responses specific to itself but modulates (e.g. , enhances) immune responses specific to a given immunogenic agent (an antigen). Adjuvant can be inorganic or organic chemical, macromolecule or whole cells of certain killed bacteria which enhance the immune response to a given antigen.

[0229] The immunogenic compositions described herein may further comprises an adjuvant. An adjuvant is an agent that modifies the immunogenicity of the glycan conjugate in the composition. Adjuvant typically does not elicit immune responses specific to it but enhances immune responses specific to a given immunogenic agent (an antigen). Adjuvant can be inorganic or organic chemical, macromolecule or whole cells of certain killed bacteria which enhance the immune response to a given antigen. In certain embodiments, the adjuvant is a mineral salt/gel, e.g., aluminium hydroxide and aluminium or calcium phosphate gels. In certain embodiments, the adjuvant is an oil-in water and water-in-oil emulsion, amphiphilic molecule and surfactant based formulation, e.g., MF59 (microfluidised detergent stabilised oil-in-water emulsion), QS- 21 (purified saponin, which is plant-derived), AS03 (consisting of an oil-in- water emulsion plus alpha-tocopherol), Montanide ISA-51 , and Montanide ISA-720. In certain embodiments, the adjuvant is liposome, virosome (unilamellar liposomal vehicles incorporating influenza haemagglutinin), ISCOMS (structured complex of saponins and lipids), and polylactide co-glycolide (PLG), PLG -Dimethylaminoethane-carbamoyl- Cholesterol

(PLGA DC-cholesterol) particles, and Iscomatrix. In certain embodiments, the adjuvant is aicrobial derivative (natural and synthetic), e.g., monophosphoryl lipid A (MPL), Detox (MPL + M. Phlei cell wall skeleton), AGP [RC-529] (synthetic acylated monosaccharide), DC Chol (lipoidal immunostimulators able to self-organise into liposomes), OM- 174 (lipid A derivative), CpG motifs (synthetic oligodeoxynucleotides containing immunostimulatory CpG motifs), modified heat labile enterotoxin (LT) and cholera toxin (CT) (genetically modified bacterial toxins that have been genetically modified to provide non-toxic adjuvant effects); synthetic dsRNA, Poly IC:LC (Hiltonol) and Poly I: Poly C12U (Ampligen®). In certain embodiments, the adjuvant is an endogenous human immunostimulator, e.g., hGM-CSF or hIL-12 (cytokines that can be administered either as protein or plasmid encoded), Immudaptin (C3d tandem array). In certain embodiments, the adjuvant is an inert vehicles, e.g., gold particle. In certain embodiments, the adjuvant is an inert polysaccharides, e.g., Advax (delta-inulin), derived from plants (dahlias). In certain embodiments, combination adjuvants or adjuvant systems can used in the immunogenic compositions described herein, for example, combinations of vaccine delivery systems and immunostimulatory agents. Combination adjuvants or adjuvant systems may result in more effective delivery of the immunostimulatory adjuvant as well as the antigen, e.g., AS01 consisting of liposomes, MPL, and QS-21; AS02 consisting of an oil-in-water emulsion plus MPL and QS-21; AS03 consisting of an oil-in-water emulsion plus alpha-tocopherol; AS04 consisting of MPL and aluminum hydroxide; AS15 consisting of liposomes, MPL, QS-21 and a CpG oligodeoxynucleotide; and GLA-SE consisting of a synthetic acylated monosaccharide in a stable oil in-water emulsion.

[0230] In some embodiments, the adjuvant used in the immunogenic compositions described herein is C34, 7DW8-5, C 17, C23, C30, a-galactoceramide, Aluminum salt, Squalene, MF59, or QS-21 (see U.S. Patent No 8268969 and U.S. Publication No. 2008-0260774, both of which are incorporated into reference in the present application).

[0231] Pharmaceutically acceptable excipients include any and all solvents, diluents, or other liquid vehicles, dispersions, suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants, and the like, as suited to the particular dosage form desired. General considerations in formulation and/or manufacture of pharmaceutical compositions agents can be found, for example, in Remington 's Pharmaceutical Sciences, Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa., 1980), and Remington: The Science and Practice of Pharmacy, 21st Edition (Lippincott Williams & Wilkins, 2005).

[0232] In certain embodiments, the immunogenic compositionsdescribed herein can be prepared, packaged, and/or sold in bulk, as a single unit dose, and/or as a plurality of single unit doses. As used herein, a "unit dose" is discrete amount of the immunogenic

compositionsdescribed herein comprising a predetermined amount of the glycan conjugate described herein.

[0233] Relative amounts of the glycan conjugate, the pharmaceutically acceptable excipient, and/or any additional ingredients in a immunogenic compositionsdescribed herein will vary, depending upon the identity, size, and/or condition of the subject treated and further depending upon the route by which the composition is to be administered. By way of example, the composition may comprise between 0.1% and 100% (w/w) active ingredient.

[0234] Pharmaceutically acceptable excipients used in the manufacture of provided

immunogenic compositionsdescribed herein include inert diluents, dispersing and/or granulating agents, surface active agents and/or emulsifiers, disintegrating agents, binding agents, preservatives, buffering agents, lubricating agents, and/or oils. Excipients such as cocoa butter and suppository waxes, coloring agents, coating agents, sweetening, flavoring, and perfuming agents may also be present in the composition.

[0235] Exemplary diluents include calcium carbonate, sodium carbonate, calcium phosphate, dicalcium phosphate, calcium sulfate, calcium hydrogen phosphate, sodium phosphate lactose, sucrose, cellulose, microcrystalline cellulose, kaolin, mannitol, sorbitol, inositol, sodium chloride, dry starch, cornstarch, powdered sugar, and mixtures thereof.

[0236] Exemplary granulating and/or dispersing agents include potato starch, corn starch, tapioca starch, sodium starch glycolate, clays, alginic acid, guar gum, citrus pulp, agar, bentonite, cellulose and wood products, natural sponge, cation-exchange resins, calcium carbonate, silicates, sodium carbonate, cross-linked poly(vinyl-pyrrolidone) (crospovidone), sodium carboxymethyl starch (sodium starch glycolate), carboxymethyl cellulose, cross-linked sodium carboxymethyl cellulose (croscarmellose), methylcellulose, pregelatinized starch (starch 1500), microcrystalline starch, water insoluble starch, calcium carboxymethyl cellulose, magnesium aluminum silicate (Veegum), sodium lauryl sulfate, quaternary ammonium compounds, and mixtures thereof.

[0237] Exemplary surface active agents and/or emulsifiers include natural emulsifiers (e.g., acacia, agar, alginic acid, sodium alginate, tragacanth, chondrux, cholesterol, xanthan, pectin, gelatin, egg yolk, casein, wool fat, cholesterol, wax, and lecithin), colloidal clays (e.g., bentonite (aluminum silicate) and Veegum (magnesium aluminum silicate)), long chain amino acid derivatives, high molecular weight alcohols (e.g., stearyl alcohol, cetyl alcohol, oleyl alcohol, triacetin monostearate, ethylene glycol distearate, glyceryl monostearate, and propylene glycol monostearate, polyvinyl alcohol), carbomers (e.g., carboxy polymethylene, polyacrylic acid, acrylic acid polymer, and carboxyvinyl polymer), carrageenan, cellulosic derivatives (e.g., carboxymethylcellulose sodium, powdered cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, methylcellulose), sorbitan fatty acid esters (e.g., polyoxyethylene sorbitan monolaurate (Tween 20), polyoxyethylene sorbitan (Tween 60), polyoxyethylene sorbitan monooleate (Tween 80), sorbitan monopalmitate (Span 40), sorbitan monostearate (Span 60], sorbitan tristearate (Span 65), glyceryl monooleate, sorbitan monooleate (Span 80)), polyoxyethylene esters (e.g., polyoxyethylene monostearate (Myrj 45), polyoxyethylene hydrogenated castor oil, polyethoxylated castor oil, polyoxymethylene stearate, and Solutol), sucrose fatty acid esters, polyethylene glycol fatty acid esters (e.g., Cremophor™), polyoxyethylene ethers, (e.g., polyoxyethylene lauryl ether (Brij 30)), poly(vinyl-pyrrolidone), diethylene glycol monolaurate, triethanolamine oleate, sodium oleate, potassium oleate, ethyl oleate, oleic acid, ethyl laurate, sodium lauryl sulfate, Pluronic F68, Poloxamer 188, cetrimonium bromide, cetylpyridinium chloride, benzalkonium chloride, docusate sodium, and/or mixtures thereof.

[0238] Exemplary binding agents include starch (e.g., cornstarch and starch paste), gelatin, sugars (e.g., sucrose, glucose, dextrose, dextrin, molasses, lactose, lactitol, mannitol, etc.), natural and synthetic gums (e.g., acacia, sodium alginate, extract of Irish moss, panwar gum, ghatti gum, mucilage of isapol husks, carboxymethylcellulose, methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose,

microcrystalline cellulose, cellulose acetate, poly(vinyl-pyrrolidone), magnesium aluminum silicate (Veegum), and larch arabogalactan), alginates, polyethylene oxide, polyethylene glycol, inorganic calcium salts, silicic acid, polymethacrylates, waxes, water, alcohol, and/or mixtures thereof.

[0239] Exemplary preservatives include antioxidants, chelating agents, antimicrobial preservatives, antifungal preservatives, alcohol preservatives, acidic preservatives, and other preservatives.

[0240] Exemplary antioxidants include alpha tocopherol, ascorbic acid, acorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, monothioglycerol, potassium metabisulfite, propionic acid, propyl gallate, sodium ascorbate, sodium bisulfite, sodium metabisulfite, and sodium sulfite.

[0241] Exemplary chelating agents include ethylenediaminetetraacetic acid (EDTA) and salts and hydrates thereof (e.g. , sodium edetate, disodium edetate, trisodium edetate, calcium disodium edetate, dipotassium edetate, and the like), citric acid and salts and hydrates thereof (e.g. , citric acid monohydrate), fumaric acid and salts and hydrates thereof, malic acid and salts and hydrates thereof, phosphoric acid and salts and hydrates thereof, and tartaric acid and salts and hydrates thereof. Exemplary antimicrobial preservatives include benzalkonium chloride, benzethonium chloride, benzyl alcohol, bronopol, cetrimide, cetylpyridinium chloride, chlorhexidine, chlorobutanol, chlorocresol, chloroxylenol, cresol, ethyl alcohol, glycerin, hexetidine, imidurea, phenol, phenoxyethanol, phenylethyl alcohol, phenylmercuric nitrate, propylene glycol, and thimerosal.

[0242] Exemplary antifungal preservatives include butyl paraben, methyl paraben, ethyl paraben, propyl paraben, benzoic acid, hydroxybenzoic acid, potassium benzoate, potassium sorbate, sodium benzoate, sodium propionate, and sorbic acid.

[0243] Exemplary alcohol preservatives include ethanol, polyethylene glycol, phenol, phenolic compounds, bisphenol, chlorobutanol, hydroxybenzoate, and phenylethyl alcohol.

[0244] Exemplary acidic preservatives include vitamin A, vitamin C, vitamin E, beta-carotene, citric acid, acetic acid, dehydroacetic acid, ascorbic acid, sorbic acid, and phytic acid. [0245] Other preservatives mclude tocopherol, tocopherol acetate, deteroxime mesylate, cetrimide, butylated hydroxyanisol (BHA), butylated hydroxytoluened (BHT), ethylenediamine, sodium lauryl sulfate (SLS), sodium lauryl ether sulfate (SLES), sodium bisulfite, sodium metabisulfite, potassium sulfite, potassium metabisulfite, Glydant Plus, Phenonip,

methylparaben, Germall 115, Germaben II, Neolone, Kathon, and Euxyl. In certain

embodiments, the preservative is an anti-oxidant. In other embodiments, the preservative is a chelating agent.

[0246] Exemplary buffering agents include citrate buffer solutions, acetate buffer solutions, phosphate buffer solutions, ammonium chloride, calcium carbonate, calcium chloride, calcium citrate, calcium glubionate, calcium gluceptate, calcium gluconate, D-gluconic acid, calcium glycerophosphate, calcium lactate, propanoic acid, calcium levulinate, pentanoic acid, dibasic calcium phosphate, phosphoric acid, tribasic calcium phosphate, calcium hydroxide phosphate, potassium acetate, potassium chloride, potassium gluconate, potassium mixtures, dibasic potassium phosphate, monobasic potassium phosphate, potassium phosphate mixtures, sodium acetate, sodium bicarbonate, sodium chloride, sodium citrate, sodium lactate, dibasic sodium phosphate, monobasic sodium phosphate, sodium phosphate mixtures, tromethamine, magnesium hydroxide, aluminum hydroxide, alginic acid, pyrogen-free water, isotonic saline, Ringer's solution, ethyl alcohol, and mixtures thereof.

[0247] Exemplary lubricating agents include magnesium stearate, calcium stearate, stearic acid, silica, talc, malt, glyceryl behanate, hydrogenated vegetable oils, polyethylene glycol, sodium benzoate, sodium acetate, sodium chloride, leucine, magnesium lauryl sulfate, sodium lauryl sulfate, and mixtures thereof.

[0248] Exemplary natural oils include almond, apricot kernel, avocado, babassu, bergamot, black current seed, borage, cade, camomile, canola, caraway, carnauba, castor, cinnamon, cocoa butter, coconut, cod liver, coffee, corn, cotton seed, emu, eucalyptus, evening primrose, fish, flaxseed, geraniol, gourd, grape seed, hazel nut, hyssop, isopropyl myristate, jojoba, kukui nut, lavandin, lavender, lemon, litsea cubeba, macademia nut, mallow, mango seed, meadowfoam seed, mink, nutmeg, olive, orange, orange roughy, palm, palm kernel, peach kernel, peanut, poppy seed, pumpkin seed, rapeseed, rice bran, rosemary, safflower, sandalwood, sasquana, savoury, sea buckthorn, sesame, shea butter, silicone, soybean, sunflower, tea tree, thistle, tsubaki, vetiver, walnut, and wheat germ oils. Exemplary synthetic oils include, but are not limited to, butyl stearate, caprylic triglyceride, capric triglyceride, cyclomethicone, diethyl sebacate, dimethicone 360, isopropyl myristate, mineral oil, octyldodecanol, oleyl alcohol, silicone oil, and mixtures thereof. [0249] Liquid dosage forms for oral and parenteral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active ingredients, the liquid dosage forms may comprise inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylene glycol, dimethylformamide, oils (e.g., cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents. In certain embodiments for parenteral administration, the glycan conjugates of the invention are mixed with solubilizing agents such as Cremophor™, alcohols, oils, modified oils, glycols, polysorbates, cyclodextrins, polymers, and mixtures thereof.

[0250] Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions can be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation can be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that can be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables.

[0251] The injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.

[0252] Compositions for rectal or vaginal administration are typically suppositories which can be prepared by mixing the glycan conjugates of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active ingredient.

[0253] Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active ingredient is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin,

polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may comprise buffering agents.

[0254] Solid compositions of a similar type can be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally comprise opacifying agents and can be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes. Solid compositions of a similar type can be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.

[0255] The glycan conjugates can be in micro-encapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the active ingredient can be admixed with at least one inert diluent such as sucrose, lactose, or starch. Such dosage forms may comprise, as is normal practice, additional substances other than inert diluents, e.g. , tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose. In the case of capsules, tablets, and pills, the dosage forms may comprise buffering agents. They may optionally comprise opacifying agents and can be of a composition that they release the glycan conjugates only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes.

[0256] Dosage forms for topical and/or transdermal administration of immunogenic compositionsdescribed herein may include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants and/or patches. Generally, the active ingredient is admixed under sterile conditions with a pharmaceutically acceptable carrier and/or any needed preservatives and/or buffers as can be required.

[0257] Suitable devices for use in delivering intradermal immunogenic compositionsdescribed herein include short needle devices such as those described in U.S. Patents 4,886,499;

5,190,521; 5,328,483; 5,527,288; 4,270,537; 5,015,235; 5,141,496; and 5,417,662. Intradermal compositions can be administered by devices which limit the effective penetration length of a needle into the skin, such as those described in PCT publication WO 99/34850 and functional equivalents thereof. Jet injection devices which deliver liquid vaccines to the dermis via a liquid jet injector and/or via a needle which pierces the stratum corneum and produces a jet which reaches the dermis are suitable. Jet injection devices are described, for example, in U.S. Patents 5,480,381; 5,599,302; 5,334,144; 5,993,412; 5,649,912; 5,569,189; 5,704,911; 5,383,851; 5,893,397; 5,466,220; 5,339,163; 5,312,335; 5,503,627; 5,064,413; 5,520,639; 4,596,556; 4,790,824; 4,941,880; 4,940,460; and PCT publications WO 97/37705 and WO 97/13537. Ballistic powder/particle delivery devices which use compressed gas to accelerate vaccine in powder form through the outer layers of the skin to the dermis are suitable. Alternatively or additionally, conventional syringes can be used in the classical mantoux method of intradermal administration.

[0258] Formulations suitable for topical administration include, but are not limited to, liquid and/or semi liquid preparations such as liniments, lotions, oil in water and/or water in oil emulsions such as creams, ointments and/or pastes, and/or solutions and/or suspensions.

Topically-administrable formulations may, for example, comprise from about 1% to about 10% (w/w) of the glycan conjugates described herein, although the concentration of the glycan conjugates can be as high as the solubility limit of the active ingredient in the solvent.

Formulations for topical administration may further comprise one or more of the additional ingredients described herein.

[0259] Immunogenic compositionsdescribed herein of the invention can be prepared, packaged, and/or sold in a formulation suitable for pulmonary administration via the buccal cavity. Such a formulation may comprise dry particles which comprise the glycan conjugates and which have a diameter in the range from about 0.5 to about 7 nanometers or from about 1 to about 6 nanometers. Such compositions are conveniently in the form of dry powders for administration using a device comprising a dry powder reservoir to which a stream of propellant can be directed to disperse the powder and/or using a self propelling solvent/powder dispensing container such as a device comprising the active ingredient dissolved and/or suspended in a low-boiling propellant in a sealed container. Such powders comprise particles wherein at least 98% of the particles by weight have a diameter greater than 0.5 nanometers and at least 95% of the particles by number have a diameter less than 7 nanometers. Alternatively, at least 95% of the particles by weight have a diameter greater than 1 nanometer and at least 90% of the particles by number have a diameter less than 6 nanometers. Dry powder compositions may include a solid fine powder diluent such as sugar and are conveniently provided in a unit dose form.

[0260] Low boiling propellants generally include liquid propellants having a boiling point of below 65 °F at atmospheric pressure. Generally the propellant may constitute 50 to 99.9% (w/w) of the composition, and the glycan conjugates may constitute 0.1 to 20% (w/w) of the composition. The propellant may further comprise additional ingredients such as a liquid non- ionic and/or solid anionic surfactant and/or a solid diluent (which may have a particle size of the same order as particles comprising the active ingredient).

[0261] Immunogenic compositionsdescribed herein of the invention formulated for pulmonary delivery may provide the glycan conjugates in the form of droplets of a solution and/or suspension. Such formulations can be prepared, packaged, and/or sold as aqueous and/or dilute alcoholic solutions and/or suspensions, optionally sterile, comprising the active ingredient, and may conveniently be administered using any nebulization and/or atomization device. Such formulations may further comprise one or more additional ingredients including, but not limited to, a flavoring agent such as saccharin sodium, a volatile oil, a buffering agent, a surface active agent, and/or a preservative such as methylhydroxybenzoate. The droplets provided by this route of administration may have an average diameter in the range from about 0.1 to about 200 nanometers.

[0262] Immunogenic compositionsdescribed herein can be useful for pulmonary delivery are useful for intranasal delivery of a immunogenic compositionsdescribed herein. Another formulation suitable for intranasal administration is a coarse powder comprising the active ingredient and having an average particle from about 0.2 to 500 micrometers. Such a formulation is administered by rapid inhalation through the nasal passage from a container of the powder held close to the nares.

[0263] Formulations for nasal administration may, for example, comprise from about as little as 0.1% (w/w) and as much as 100% (w/w) of the glycan conjugates provided herein, and may comprise one or more of the additional ingredients described herein. An immunogenic compositionsdescribed herein can be prepared, packaged, and or sold in a formulation for buccal administration. Such formulations may, for example, be in the form of tablets and/or lozenges made using conventional methods, and may contain, for example, 0.1 to 20% (w/w) active ingredient, the balance comprising an orally dissolvable and/or degradable composition and, optionally, one or more of the additional ingredients described herein. Alternately, formulations for buccal administration may comprise a powder and/or an aerosolized and/or atomized solution and/or suspension comprising the active ingredient. Such powdered, aerosolized, and/or aerosolized formulations, when dispersed, may have an average particle and/or droplet size in the range from about 0.1 to about 200 nanometers, and may further comprise one or more of the additional ingredients described herein.

[0264] An immunogenic compositionsdescribed herein can be prepared, packaged, and/or sold in a formulation for ophthalmic administration. Such formulations may, for example, be in the form of eye drops including, for example, a 0.1/1.0% (w/w) solution and/or suspension of the glycan conjugate in an aqueous or oily liquid carrier. Such drops may further comprise buffering agents, salts, and/or one or more other of the additional ingredients described herein.

[0265] Although the descriptions of immunogenic compositions described herein are principally suitable for administration to humans, it will be understood by the skilled artisan that such compositions are generally suitable for administration to animals of all sorts. Modification of immunogenic compositionsdescribed herein suitable for administration to humans in order to render the compositions suitable for administration to various animals is well understood, and the ordinarily skilled veterinary pharmacologist can design and/or perform such modification with ordinary experimentation.

[0266] Immunogenic compositions described herein provided herein are typically formulated in dosage unit form for ease of administration and uniformity of dosage. It will be understood, however, that the total daily usage of the immunogenic compositionsdescribed herein will be decided by the attending physician within the scope of sound medical judgment. The quantity to be administered also depends on the subject to be treated, including, for example, the capacity of the individual's immune system to synthesize antibodies, and if needed, to produce a cell- mediated immune response. Suitable regimes for initial administration and booster doses are also variable, but may include an initial administration followed by subsequent administrations. The specific therapeutically effective dose level for any particular subject or organism will depend upon a variety of factors including the disease, disorder, or condition being treated and the severity of the disorder; the activity of the specific active ingredient employed; the specific composition employed; the age, body weight, general health, sex and diet of the subject; the time of administration, route of administration, and rate of excretion of the specific active ingredient employed; the duration of the treatment; drugs used in combination or coincidental with the specific active ingredient employed; and like factors well known in the medical arts.

[0267] The compounds and compositions provided herein can be administered by any route, including enteral (e.g., oral), parenteral, intravenous, intramuscular, intra-arterial,

intramedullary, intrathecal, subcutaneous, intraventricular, transdermal, interdermal, rectal, intravaginal, intraperitoneal, topical (as by powders, ointments, creams, and/or drops), mucosal, nasal, bucal, sublingual; by intratracheal instillation, bronchial instillation, and/or inhalation; and/or as an oral spray, nasal spray, and/or aerosol. Specifically contemplated routes are oral administration, intravenous administration (e.g., systemic intravenous injection), regional administration via blood and/or lymph supply, and/or direct administration to an affected site. In general the most appropriate route of administration will depend upon a variety of factors including the nature of the agent (e.g., its stability in the environment of the gastrointestinal tract), and/or the condition of the subject (e.g., whether the subject is able to tolerate oral administration).

[0268] It will be appreciated that dose ranges as described herein provide guidance for the administration of provided pharmaceutical compositions to an adult. The amount to be administered to, for example, a child or an adolescent can be determined by a medical practitioner or person skilled in the art and can be lower or the same as that administered to an adult.

[0269] Also encompassed by the invention are kits (e.g. , pharmaceutical packs) to treat or reduce the risk of bacterial infections. The kits provided may comprise an inventive

pharmaceutical composition or compound and a container (e.g. , a vial, ampule, bottle, syringe, and/or dispenser package, or other suitable container). The kits provided may comprise an additional therapeutically active agents include, but are not limited to, antibiotics, anti-viral agents, anesthetics, anti-coagulants, inhibitors of an enzyme, steroidal agents, steroidal or nonsteroidal anti-inflammatory agents, antihistamine, immunosuppressant agents, antigens, vaccines, antibodies, decongestant, sedatives, opioids, pain-relieving agents, analgesics, antipyretics, hormones, and prostaglandins, etc. In some embodiments, provided kits may optionally further include a second container comprising a pharmaceutical excipient for dilution or suspension of an inventive pharmaceutical composition or compound. In some embodiments, the inventive pharmaceutical composition or compound provided in the container and the second container are combined to form one unit dosage form.

Uses of Glycan Conjugates Described Herein

[0270] The present invention provides glycan conjugates and immunogenic compositions comprising such for use in treating or reducing the risk of an infectious disease such as bacterial infection in a subject. In certain embodiments, a glycan conjugate population as described herein, either homogenous or heterogenous, is provided in an effective amount in the immunogenic composition. In certain embodiments, the effective amount is a therapeutically effective amount. In certain embodiments, the effective amount is a prophylactically effective amount. The glycan conjugates or immunogenic compositions described herein may be effective in treating the infection, delaying onset of the infection, or reducing the risk for the infection. In certain embodiments, the effective amount is an amount effective in eliciting immune responses specific to a bacterium, e.g., a bacterium from which the oligosaccharide antigen in the immunogenic composition is derived. In certain embodiments, the effective amount is therapeutically effective amount, for example, the amount is sufficient for inhibiting bacterial growth or alleviating a condition caused by the infection. In certain embodiments, the bacterium which is the causative agent of the infection is a Gram-negative bacterium. In certain embodiments, the bacterium is a Gram-positive bacterium. In certain embodiments, the bacterium is N. meningitides . In certain embodiments, the bacterium is N. meningitides serogroup W135.

[0271] The immunogenic compositionsdescribed herein can also be used to generate antibodies in human or animals for production of antibodies, which can be used in both treatment and diagnosis of the infectious disease. In some embodiments, the immunogenic

compositionsdescribed herein can also be used to generate antibodies for production of N. meningitidis antibodies. In some embodiments, the immunogenic compositionsdescribed herein can also be used to generate antibodies for production of N. meningitidis serogroup W135 antibodies. Methods of making monoclonal and polyclonal antibodies and fragments thereof in human and/or animals (e.g., mouse, rabbit, goat, sheep, or horse) are well known in the art. See, for example, Harlow and Lane, (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, New York. The term "antibody" includes intact immunoglobulin molecules as well as fragments thereof, such as Fab, F(ab').sub.2, Fv, scFv (single chain antibody), and dAb (domain antibody; Ward, et. al. (1989) Nature, 341, 544).

[0272] In some embodiments, the provided glycan conjugates, immunogenic compositionsare useful in treating and/or reducing the risk of an infectious disease. In some embodiments, the provided glycan conjugates, immunogenic compositionsthereof are useful to delay the onset of, slow the progression of, or ameliorate the symptoms of the infectious disease. In some embodiments, the provided glycan conjugates, immunogenic compositionsare administered in combination with other compounds, drugs, or therapeutics to treat or reduce the risk of the infectious disease.

[0273] In yet another aspect, provided here is a method of treating or reducing the risk of a bacterial infection caused by bacteria that are resistant to other treatments. In certain embodiments, provided is a method of treating or reducing the risk of a bacterial infection caused by bacteria that are multi-drug tolerant. In certain embodiments, provided is a method of treating or preducing the risk of a bacterial infection caused by bacteria that are multi-drug resistant. In certain embodiments, provided is a method of treating or preducing the risk of a bacterial infection caused by bacteria that neither grow nor die in the presence other treatments. In certain embodiments, provided is a method of treating or preducing the risk of a bacterial infection caused by bacteria that neither grow nor die as a result of other treatments. In certain embodiments, provided methods can be conducted in vivo (i.e., by administration to a subject). For example, in certain embodiments, provided is a method of treating and/or reducing the risk of a bacterial infection. The method may comprise administering an effective amount of a glycan conjugate, immunogenic composition thereof as described herein to a subject with a bacterial infection or at risk of developing a bacterial infection.

[0274] In another aspect, the present invention provides a method of killing bacteria in a subject comprising administering an effective amount of glycan conjugate, immunogenic composition as described herein.

[0275] In certain embodiments, the bacterial infection being treated is an infection with a Gram positive bacterium. In certain embodiments, the Gram positive bacterium is a bacterium of the phylum Firmicutes. In certain embodiments, the bacteria is a member of the phylum Firmicutes and the genus Enterococcus, i.e., the bacterial infection is an Enterococcus infection. Exemplary Enterococci bacteria include, but are not limited to, E. avium, E. durans, E.faecalis, E.faecium, E. gallinarum, E. solitarius, E. casseliflavus, a.nd E. raffinosus. In certain embodiments, the Enterococcus infection is an E.faecalis infection. In certain embodiments, the Enterococcus infection is an E. faecium infection.

[0276] In certain embodiments, the bacterial infection being treated is an infection with a Gram negative bacterium. In certain embodiments, the Gram negative bacteria species is selected from the group consisting of Staphylococcus sp., Enterococcus sp., Escherichia coli, Bacillus sp., Salmonella sp., Mycobacterium sp., and meningococcus. In certain embodiments, the Gram negative bacteria species is N. meningitides. In certain embodiments, the Gram negative bacteria species is N. meningitides serogroup W135.

[0277] In certain embodiments, the bacterial infection is resistant to other antibiotic therapy. For example, in certain embodiments, the bacterial infection is vancomycin resistant (VR). In certain embodiments, the bacterial infection is a vancomycin-resistant E. faecalis infection. In certain embodiments, the bacterial infection is a vancomycin-resistant E. faecium infection. In certain embodiments, the bacterial infection is vancomycin-resistant Staphylococcus aureus (VRSA). In certain embodiments, the bacterial infection is vancomycin-resistant Enterococci (VRE). In certain embodiments, the bacterial infection is a methicillin-resistant (MR). In certain embodiments, the bacterial infection is a methicillin-resistant S. aureus (MRSA) infection. In certain embodiments, the bacterial infection is methicillin-resistant Staphylococcus epidermidis (MRSE). In certain embodiments, the bacterial infection is penicillin-resistant Streptococcus pneumonia. In certain embodiments, the bacterial infection is quinolone-resistant Staphylococcus aureus (QRSA). In certain embodiments, the bacterial infection is multi-drug resistant Mycobacterium tuberculosis as.

[0278] To perform the treatment methods described herein, an effective amount of any of the glycan conjugates or immunogenic compositions described herein may be administered to a subject in need of the treatment via a suitable route, as described above . The subject, such as a human subject, can be a patient having an infectious disease (e.g., bacterial infection), suspected of having an infectious disease, or susceptible to an infectious disease. The amount of the glycan conjugate or immunogenic composition admintered to the subject may be effective in eliciting immune responses specific to the glycan moiety in the conjugate or composition. In some embodiments, the amount of the glycan conjugate or immunogenic composition is sufficient to elicit immune responses leading to the inhibition of bacterial growth and/or reduction of bacteria population. In other embodiments, the amount of the glycan conjugate or immunogenic composition may be effective in delaying the onset of the infectious disease or reducing the risk for developing an infectious disease.

[0279] The exact amount of the provided glycan conjugates, immunogenic

compositionsrequired to achieve an effective amount will vary from subject to subject, depending, for example, on species, age, and general condition of a subject, severity of the side effects or disorder, identity of the particular compound(s), mode of administration, and the like. The desired dosage can be delivered three times a day, two times a day, once a day, every other day, every third day, every week, every two weeks, every three weeks, or every four weeks. In certain embodiments, the desired dosage can be delivered using multiple administrations (e.g. , two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, or more administrations) .

[0280] In certain embodiments, an effective amount, protective amount, or immunogenic amount, of the provided glycan conjugates, immunogenic compositionsfor administration one or more times a day to a 70 kg adult human may comprise about 0.0001 mg to about 3000 mg, about 0.0001 mg to about 2000 mg, about 0.0001 mg to about 1000 mg, about 0.001 mg to about 1000 mg, about 0.01 mg to about 1000 mg, about 0.1 mg to about 1000 mg, about 1 mg to about 1000 mg, about 1 mg to about 100 mg, about 10 mg to about 1000 mg, or about 100 mg to about 1000 mg, of a compound per unit dosage form.

[0281] In certain embodiments, the provided glycan conjugates, immunogenic compositionsmay be administered orally or parenterally at dosage levels sufficient to deliver from about 0.001 mg/kg to about 100 mg/kg, from about 0.01 mg/kg to about 50 mg/kg, preferably from about 0.1 mg/kg to about 40 mg/kg, preferably from about 0.5 mg/kg to about 30 mg kg, from about 0.01 mg/kg to about 10 mg/kg, from about 0.1 mg/kg to about 10 mg/kg, and more preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.

[0282] It will be appreciated that dose ranges as described herein provide guidance for the administration of the provided glycan conjugates, immunogenic compositionsto an adult. The amount to be administered to, for example, a child or an adolescent can be determined by a medical practitioner or person skilled in the art and can be lower or the same as that

administered to an adult.

[0283] It will be also appreciated that the provided glycan conjugates, immunogenic compositionscan be administered in combination with one or more additional therapeutically active agents. The the provided glycan conjugates, immunogenic compositionscan be administered in combination with additional therapeutically active agents that improve their bioavailability, reduce and/or modify their metabolism, inhibit their excretion, and/or modify their distribution within the body. It will also be appreciated that the therapy employed may achieve a desired effect for the same disorder, and/or it may achieve different effects.

[0284] The the provided glycan conjugates, immunogenic compositionscan be administered concurrently with, prior to, or subsequent to, one or more additional therapeutically active agents. In general, each agent will be administered at a dose and/or on a time schedule determined for that agent. In will further be appreciated that the additional therapeutically active agent utilized in this combination can be administered together in a single composition or administered separately in different compositions. The particular combination to employ in a regimen will take into account compatibility of the inventive compound with the additional therapeutically active agent and/or the desired therapeutic effect to be achieved. In general, it is expected that additional therapeutically active agents utilized in combination be utilized at levels that do not exceed the levels at which they are utilized individually. In some embodiments, the levels utilized in combination will be lower than those utilized individually.

[0285] In certain embodiments, the provided glycan conjugate or immunogenic composition is administered in combination with one or more additional therapeutically active agents described herein.

[0286] Exemplary additional therapeutically active agents include, but are not limited to, antibiotics, anti-viral agents, anesthetics, anti-coagulants, inhibitors of an enzyme, steroidal agents, steroidal or non-steroidal anti-inflammatory agents, antihistamine, immunosuppressant agents, antigens, vaccines, antibodies, decongestant, sedatives, opioids, pain-relieving agents, analgesics, anti-pyretics, hormones, and prostaglandins, etc. Therapeutically active agents include small organic molecules such as drug compounds (e.g., compounds approved by the US Food and Drug Administration as provided in the Code of Federal Regulations (CFR)), peptides, proteins, carbohydrates, monosaccharides, oligosaccharides, polysaccharides, nucleoproteins, mucoproteins, lipoproteins, synthetic polypeptides or proteins, small molecules linked to proteins, glycoproteins, steroids, nucleic acids, DNAs, RNAs, nucleotides, nucleosides, oligonucleotides, antisense oligonucleotides, lipids, hormones, vitamins and cells.

[0287] In certain embodiments, the additional therapeutically agent is an antibiotic. Exemplary antibiotics include, but are not limited to, penicillins (e.g. , penicillin, amoxicillin),

cephalosporins (e.g. , cephalexin), macrolides (e.g. , erythromycin, clarithormycin, azithromycin, troleandomycin), fluoroquinolones (e.g. , ciprofloxacin, levofloxacin, ofloxacin), sulfonamides (e.g. , co-trimoxazole, trimethoprim), tetracyclines (e.g. , tetracycline, chlortetracycline, oxytetracycline, demeclocycline, methacycline, sancycline, doxycline, aureomycin, terramycin, minocycline, 6-deoxytetracycline, lymecycline, meclocycline, methacycline, rolitetracycline, and glycylcycline antibiotics (e.g. , tigecycline)), aminoglycosides (e.g. , gentamicin, tobramycin, paromomycin), aminocyclitol (e.g. , spectinomycin), chloramphenicol, sparsomycin, quinupristin/dalfoprisin (Syndercid™). In certain embodiments, the additional additional therapeutically agent is third-generation cephalosporin antibiotics (i. e. cefotaxime, ceftriaxone) or rifampicin.

[0288] In certain embodiments, the subject being treated is a mammal. In certain embodiments, the subject is a human. In certain embodiments, the subject is a domesticated animal, such as a dog, cat, cow, pig, horse, sheep, or goat. In certain embodiments, the subject is a companion animal such as a dog or cat. In certain embodiments, the subject is a livestock animal such as a cow, pig, horse, sheep, or goat. In certain embodiments, the subject is a zoo animal. In another embodiment, the subject is a research animal such as a rodent, dog, or non-human primate. In certain embodiments, the subject is a non-human transgenic animal such as a transgenic mouse or transgenic pig.

[0289] Without further elaboration, it is believed that one skilled in the art can, based on the above description, utilize the present invention to its fullest extent. The following specific embodiments are, therefore, to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever. All publications cited herein are incorporated by reference for the purposes or subject matter referenced herein.

EXAMPLES

[0290] In order that the invention described herein may be more fully understood, the following examples are set forth. It should be understood that these examples are for illustrative purposes only and are not to be construed as limiting this invention in any manner. [0291] All chemicals were from commercial sources in reagent grade and used without further purification. Ethyl acetate and hexane were purified by distillation, and water was Mill-Q- purified. Anhydrous dichloromethane (CH₂CI₂) was also used without purification or distillation. All reactions were performed under argon atmosphere. Pulverized molecular sieves MS-4A (Aldrich) for glycosylation was activated by heating at 350°C for 3~6 hours. Reactions were monitored by thin-layer chromatography (TLC) analysis, which was performed on glass plates pre-coated with silica gel 60 F254 (0.25 mm, Merck). The TLC was detected by UV light (254 nm), p-anisaldehyde A, or eerie ammonium molybdate. The products were purified by flash chromatography with silica gel (Merck, 40-63 μηι size). ¾ NMR and ¹³C NMR sprecta were recorded on Bruker AVANCE 600 (600 MHz) spectrometer at 25°C . Chemical shift on ^!H NMR was assigned according to TMS (δ= 0 ppm, in CDC1₃), CD₃OD (δ = 3.3 ppm), and D₂0 (δ = 4.8 ppm). Chemical shifts measurements are reported in delta (δ) units, and splitting patterns are described as singlet (s), doublet (d), triplet (t), quartet (q), or multiplet (m). Coupling constants (J) are reported in Hertz (Hz). High resolution ESI mass spectra were recorded on a Bruker Daltonics or Brucker Bio-TOF III spectrameter. MALDI specta were recorded on Bruker Ultraflex II sepctrameter. To fabricating the microarray, amine-contained oligosaccharide were prepared by dissolving in the printing buffer (300 mM phosphate buffer, 0.005% Tween 20, pH 8.5) in 10 mM concentration. Microarrays were printed (BioDot; Cartesian Technologies) by robotic pin (SMP3; TeleChem International) deposition of ~0.6 nL of various solutions from 96-well plate onto NHS-coated glass slide (NexterionHslide; SCHOTTNorthAmerica). Alexa Fluor 647-conjugated goat anti-mouse IgG antibody, DyLight 649-conjugated goat anti-mouse IgM antibody, Alexa Fluor 488- conjugated goat anti-mouse IgGl antibody, Alexa Flour 594- conjugated goat anti-mouse IgG2a antibody, Cy3- conjugated goat anti-mouse IgG2b antibody, Alexa Flour 488-conjugated anti-mouse IgG2c antibody, and Alexa Fluor 647- conjugated goat anti-mouse IgG3 antibody were purchased from Jackson ImmunoResearch. The micoarray slides were scanned at 635 nm, 594 nm, 532 nm, or 488 nm wavelength with a microarray

fluorescence chip reader (Array Worx microarray reader). The fluorescence data were analyzed by GenePix Pro software (Axon Instruments).

Synthesis of glycan conjugates and related compounds

[0292] Synthesis of sialic acid building block. The N-acetylated thioglycoside compound 2 was synthesized by the reported three steps method using the known thio sialoside compound 1 as a starting material. In order to increase -stereoselectivity and yield of the following sialylation reaction, dibutyl phosphate group was introduced to replace thiophenyl group in the anomeric center of thioglycoside 2 at 0 °C under N-iodosuccinimide (NIS) and

trifluoromethanesulfonic acid (TfOH) activation ovenight. Both a- and β- phosphate sialoside isomers were obtained in a 1/2 mixture in 82% yield (Scheme 1). The configuration of a and β sialyl phosphate (compound 3 and 4) was determined by NM spectometer examination. ³J(Ci- Ebax) = 5.7 Hz of compound 3 for a isomer, while ³J(Ci-H3_ax) coupling constant of the β phosphate sialoside (compound 4) was too small to be detected.

Scheme 1. Synthesis of sialic acid building block 3 and 4. Reagent and conditions: a) TBDPSC1, Imidazole, DMF, r.t.; (b) DMP, CSA, CH₂C1₂, r.t, 92%, two steps; (c) AcCl, DMAP, CH₂C1₂, 0 °C, 85%; (d) dibutyl phosphate, NIS, TfOH, CH₂C1₂, 0 °C, 82%, α : β = 1 :2.

[0293] Synthesis of galactose building block. The 2,3,4-O-tribenzyl thiol galactoside compound 5 was synthesized by the reported method with a minor modification (H. Dohi, R. Perion, M. Durka, M. Bosco, Y. Roue, F. Moreau, S. Grizot, A. Ducruix, S. Escaich, S. P.

Vincent, Chem-Eur J 2008, 14, 9530-9539). For the purpose of conjugation with carrier protein and immobilization on the microarray, a 5-azido-pentanol linker was installed on the galactose anomeric position with a linkage by adopting thiol galactoside 6, which was reported to direct high -selectivity as the result of steric effect by the 4,6-di-tert-butylsilylene (DTBS) protection group. Glycosylaion of compound 6 and 5-azido-l-pentanol under NIS/TfOH activation at - 40°C in CH₂C1₂ for 1 hour gave galactoside 7 in 86% yield and only a form. The DTBS group was removed in the presence of TBAF in THF overnight to obtain compound 8 in 81% yield. Compound 9 was synthesized by sequentially 4,6-benzylidene protection and selective ring

Scheme 2. Synthesis of galactose building block 9. Reagent and conditions: a) 5-azidopentan- l-ol, NIS, TfOH, CH₂C1₂, -40 °C, 86%; b) TBAF, THF, r.t, 81%; c) BDA, CSA, DMF, r.t, quant.; d) BF₃ · THF, TMSOTf, CH₂C1₂, 0 °C, 72%. [0294] Synthesis of disaccharide building block. The reactive condition of a- and β- phosphate sialoside with galactoside was explored. The a-phosphate sialoside (compound 3) reacted with compound 5 under the activation of TMSOTf in CH₂CI₂ at -78 °C for 10 minutes to give a Neu5Ac-a-(2→6)-Gal disaccharide 10 as a single isomer in 92% yield (Scheme 3). The configuration of the disaccharide was examined by NMR spectrometer, and the new formed - glycosidic bond was confirmed by V(Ci-H_3ax) = 5.7 Hz coupling constant. Although a previous report indicated that β-phosphate sialoside was less reactive than a-phosphate sialoside and required higher temperature for activation (C. H. Hsu, K. C. Chu, Y. S. Lin, J. L. Han, Y. S. Peng, C. T. Ren, C. Y. Wu, C. H. Wong, Chem-Eur J 2008, 16, 1754-1760), it was found that using galactoside 5 as an acceptor, the β-phosphate sialoside 4 was fully activated even with temperature as low as -70 °C. The a-isomer was also obtained as product 11 in 86% yield by

3, a - form; 4, β -form 5, R= β - STol 10, R= β -STol

9, R= a - 5-azido-pentanol 11 , R= α-5-azido-pentnol

Scheme 3. Synthesis of disaccharide building block 10 and 11. Reagent and conditions: a) TMSOTf, CH₂C1₂, -70 °C, about 90%.

[0295] Oligosaccharide elongation. The oligosaccharide elongation was done via an iterative glycosylation and deprotectoin strategy by using the synthesize disaccharide 10 as a common donor for [2+n] glycosylation reaction to construct oligosaccharides up to decasaccharide. Ccompound 11 was used as a reducing end building block and selectively removed its oxazolidione ring under the Zemplen condition to obtain product 12 in 78% yield. Glycosylation of the disaccharide donor 10 and alcohol acceptor 12 in the presence of NIS/TfOH activation in CH₂CI₂ at -40°C for 1 hour gave fully protected tetrasaccharide 14 in 64% yield. Compound 14 was further undergone the Zemplen condition to open the oxazolidinoe ring to give

tetrasaccharide accepotor 1 in 75% yield. The fully protected hexasaccharide 17 was synthesized by the [2+4] glycosylation using 10 and 15 with 52% yield and single steroisomer. Repeating the oxazolidione ring opening and the same [2+n] glycosyaltion startegy, octasaccharide 20 and decasaccharide 23 were synthesized. But with the increasing length of the oligosaccharide, the yields decreased to 46% and 35%, respectively. These products are also obtained in single a isomer. The alcohol product of hexasaccharide 18, octasaccharide 21, and decasacchride 24 were obtained from the fully protected oligosacchride under the Zemplen condition in 70~80% yield (Figure 4).

[0296] Finally, the total deprotected compounds can be obaind by global deprotection methods from the alcohol compounds of 12, 15, 18, 21 and 24. First, the TBDPS group and

isopropylidene group of the alcohol compounds were removed in the presence of excess BF3 · OEt₂ at 0°C for 3 hours owing to the fluoride and acidic property of BF3. Then, the methyl ester group was removed under strong base NaOH in MeOH. Finally, all benzyl groups were removed through hydrogenation reaction under catalyst Pd(OH)₂ and H₂ in MeOH/H₂0. The final deprotected products 13, 16, 19, 22, and 25 can be obtained in 45%~60% yield over three steps (Scheme 4). Notably, the estimated coupling constant value of galactose anomeric proton was about 3~4 Hz. The small coupling constant confirmed that a-linked Gal-(l→4)-Neu5Ac oligosaccharide was obtained.

[0297] Carbohydrate-protein conjugation. Many chemical approaches have been developed to crosslink carbohydrate and protein: (1) the Staudinger ligation employes a substituted phosphite to react with the azide-modified protein to form the carbohydrate-protein conjugation via the amide bond formation (C. Grandjean, A. Boutonnier, C. Guerreiro, J. M. Fournier, L. A. Mulard, J Org Chem 2005, 70, 7123-7132); (2) oxime conjugation introduces an aminooxy group on the protein to react with oligosaccharide containing aldehyde or keto group (J. Kubler- Kielb, V. Pozsgay, J Org Chem 2005, 70, 6987-6990); (3) frequently used Michael addition often uses thiol group addition to maleimide to form a stable thioester linkage (T. Masuko, A. Minami, N. Iwasaki, T. Majima, S. Nishimura, Y. C. Lee, Biomacromolecules 2005, 6, 880-884); and (4) recently, the method of copper (I)-catalyzed cycloaddition of azide to alkyens (click chemistry) provides efficient glycoconjugation (a) H. C. olb, M. G. Finn, K. B. Sharpless, Angew Chem Int Ed Engl 2001 , 40, 2004-2021 ; b) S. Hotha, S. Kashyap, J Org Chem 2006, 71, 364-367). However, the triazole ring produces undesired immunogenicity. Thus, the thiol- maleimide coupling method was adopted for carbohydrate-protein conjugation for its high efficiency in sialic acid-rich compounds. 3,3-dithiobis sulfosuccinimidylpropionate (DTSSP) was allowed to react with the deprotected amino compounds 13, 16, 19, 22, and 25 in pH 7.4 PBS buffer overnight. The disulfide bond was cleaved in the present of dithiothreitol (DTT) at 40 °C for 1 hour to obtain the free thio products 32, 33, 34, 35, and 36 as Michael donors in 70-75% yield. To generate maleimide reactive group on the protein, CRM 197 was reacted with sulfo-EMCS in pH 8.0 PBS buffer for 1 hour. The number of maleimide-linker on the protein was determined by MALDI-TOF mass spectrometer. In average, 20 maleimide linkers were coupled on diphtheria toxin mutant CRM 197. Oligosaccharides were incorporated into carrier protein CRM197 by conjugating thiol modified oligosaccharide products 32, 33, 34, 35, and 36 and maleimide modified CRM 197 in pH 7.4 PBS buffer for 1 hour (Figure 5) to obatain the disaccharide- to decasaccharide-glycoconjugates DT-2, DT-4, DT-6, DT-8, and DT- 10 with various carbohydrate epitope on DT. Again, the number of oligosaccharides conjugating to protein DT was determined by MALDI-TOF mass spectrometer (Table 1).

Table 1. The number of oligosaccharide conjugating to protein

Mean number of oligosaccharide coupled onto the carrier protein. The longer oligosaccharides have lower coupling number due to the structure effect.

Synthetic procedures

Methyl-(5-acetamino-9-0-¾i"i-butyldipheny]silyl-7,8-0-isopropylidene-5-^V,4-0-carbonyl- 3,5-dideoxy-2-thio-D-glycero-a-D-galacto-non-2-ulopyranoside)onate (2)

[0298] A mixture of imidazole (1.36 g, 20.03 mmol) and compound 1 (4 g, 10.01 mmol) was dissolved in dry DMF (40 mL). The solution was cooled to 0 °C, followed by adding tert- butylchlorodiphenylsilane (3.3 mL, 12.01 mmol). The reaction was stirred overnight at room temperature. After reaction, the solvent was removed under reduced pressure. The residue was diluted with EtOAc and washed with IN HCl_(aq), saturated aHC0_3(aq), and brine. The organic layer was dried over MgS0₄ and concentrated. Then, camphorsulfonic acid (2.32 g, 10.01 mmol) and 2,3-dimethoxypropane (40 mL) was added to the residue. The reaction was stirred at room temperature for 3 hours. After reaction, the solution was quenched with Et₃N (2 mL, 15 mmol), and the solvent was removed under reduced pressure. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/3). Purified compound (5 g, 7.37 mmol) was dissolved in CH₂C1₂ (50 ml) and cooled to 0 °C. NN-Diisopropylethylamine (12.18 mL, 73.7 mmol) and acetyl chloride (4.21 mL, 59 mmol ) was added sequentially and slowly at 0 °C. Overnight, the reaction was stirred from 0 °C to room temperature. After reaction, the solution was washed with saturated aHC0_3(aq), and brine. The organic layer was dried over MgSC and concentrated. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/5) to give compound 2 (4.51 g, 85%): *H NMR (600 MHz, CDC1₃) δ 7.69 (dd, J= 8.2, 1.2 Hz, 2H), 7.62 - 7.55 (m, 4H), 7.43 - 7.36 (m, 2H), 7.35 - 7.27 (m, 5H), 7.27 - 7.21 (m, 2H), 4.98 (d, J= 7.1 Hz, 1H), 4.36 (dd, J= 12.9, 6.9 Hz, 1H), 4.18 (dd, J= 10.8, 7.2 Hz, 1H), 4.10 - 3.96 (m, 3H), 3.83 (ddd, J= 12.7, 1 1.2, 3.4 Hz, 1H), 3.27 (s, 3H), 3.16 (dd, J= 11.8, 3.4 Hz, 1H), 2.49 (s, 3H), 2.14 (t, 1H), 1.77 (s, 3H), 1.43 (s, 3H), 1.00 (s, 9H). ¹³C NMR (150 MHz, CDC1₃) δ 172.43, 167.94, 153.85, 136.82, 135.59, 133.82, 133.76, 130.14, 129.65, 129.52, 128.68, 128.61, 127.63, 127.55, 109.76, 88.76, 78.32, 77.77, 76.93, 75.50, 61.96, 59.84, 52.44, 36.68, 26.78, 26.50, 25.86, 25.21 , 19.18. HRMS (ESI-TOF) calcd. for CasHis OsSSiNa [M+Na]⁺: 742.2477, found: 742.2476.

Methyl-(5-acetamino-9-0-teri-butyldiphenylsilyl-7,8-0-isopropylidene-5-/V,4-i?-carbony]-2- dibutylphosphoryl-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate (3, a form)

[0299] A mixture of compound 2 (3 g, 4.17 mmol), dibutyl phosphate (2.63 g, 12.5 mmol), and activated pulverized MS-4A in dry CH₂CI₂ (30 mL) was stirred at room temperature for 3 hours. The solution was cooled to 0 °C and sequentially added N-iodosuccinimide (1.8 g, 8.32 mmol) and 0.5M trifiuoromethanesulfonic acid in ether (1.67 mL, 0.834 mmol). The reaction was stirred at 0 °C overnight. After reaction, the solution was filtered through celite. The filtrate was then quenched with 20% Na2S203(_aq) and wash with saturated NaHC03(_aq) and brine. The organic layer was dried over MgSC>4 and concentrated. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/5 to 1/3) to give compound 3 and 4 (2.80 g, 82%, α/β=1/2).

[0300] ^JH NMR (600 MHz, CDC1₃) δ 7.76 - 7.62 (m, 4H), 7.48 - 7.30 (m, 6H), 4.93 (dd, J= 7.1, 1.3 Hz, 1H), 4.42 (dd, J = 9.2, 1.4 Hz, 1H), 4.32 (q, J= 6.4 Hz, 1H), 4.18 (dd, J = 10.9, 6.4 Hz, 1H), 4.16 - 4.09 (m, 2H), 4.09 - 3.96 (m, 5H), 3.64 (s, 3H), 3.10 (dd, J = 11.9, 3.5 Hz, 1H), 2.49 (s, 3H), 2.35 - 2.26 (m, 1H), 1.68 - 1.58 (m, 4H), 1.50 (s, 3H), 1.42 - 1.32 (m, 7H), 1.04 (s, 9H), 0.91 (dt, J= 15.9, 7.4 Hz, 6H). ¹³C NMR (150 MHz, CDCI₃) δ 172.49, 167.44, 154.00, 135.65, 135.62, 133.76, 133.61, 129.73, 129.66, 127.72, 127.68, 109.25, 98.63, 98.60, 77.58, 77.56, 76.39, 74.23, 68.35, 68.31, 67.94, 67.90, 62.57, 59.54, 53.13, 36.86, 32.07, 32.04, 26.85, 26.27, 25.48, 25.10, 19.23, 18.61, 18.58, 13.56. HRMS (ESI-TOF) calcd. for

[M+Na]⁺: 842.3307, found: 842.3351.

Methyl-(5-acetamino-9-0-teri-butyldiphenylsilyl-7,8-0-isopropylidene-5-A^r,4-i>-carbony]-2- dibutylphosphoryl-3,5-dideoxy-D-glycero-P-D-galacto-non-2-ulopyranoside)onate (4, β form)

[0301] *H NMR (600 MHz, CDC1₃) δ 7.73 - 7.59 (m, 4H), 7.47 - 7.34 (m, 6H), 4.96 (d, J= 7.6 Hz, 1H), 4.48 (ddd, J= 12.9. 11.4, 3.6 Hz, 1H), 4.36 (d, J= 9.4 Hz, 1H), 4.29 (dd, J= 12.9, 6.9 Hz, 1H), 4.14 - 4.08 (m, 2H), 4.08 - 3.98 (m, 3H), 3.96 - 3.87 (m, 2H), 3.75 (s, 3H), 3.01 (dd, J = 13.2, 3.6 Hz, 1H), 2.59 (t, J= 13.1 Hz, 1H), 2.53 (s, 3H), 1.65 - 1.58 (m, 2H), 1.57 - 1.50 (m, 2H), 1.40 - 1.29 (m, 10H), 1.05 (d, J= 5.6 Hz, 9H), 0.90 (dt, J= 17.9, 7.4 Hz, 6H). "C NMR (150 MHz, CDC1₃) δ 172.57, 165.38, 153.93, 135.54, 135.50, 133.57, 133.53, 129.80, 129.73, 127.78, 127.75, 109.48, 99.97, 99.95, 77.32, 76.38, 76.20, 74.52, 68.30, 68.26, 68.14, 68.10, 62.44, 59.49, 53.24, 35.02, 32.17, 32.12, 32.07, 26.86, 25.91, 25.66, 25.02, 19.27, 18.61, 18.58, 13.57, 13.52. HRMS (ESI-TOF) calcd. for

[M+Na : 842.3307, found:

842.3351.

4,6-0-di-fert-butylsilanediyl-2,3-6>-di-benzy]-l-t>-(5-azidopenty)-a-D-galactopyranoside (7)

[0302] Compound 6 was synthesized as described. A mixture of compound 6 (5 g, 8.23 mmol), 5-azidopentan-l-ol (2.3 g, 16.46 mmol), and activated pulverized MS-4A in dry CH₂CI₂ (50 ml) was stirred at room temperature for 3 hours. The solution was cooled to 0°C, and N- iodosuccinimide (1.8 g, 16.46 mmol) was added. After that, the solution was cooled to -40°C, followed by adding 0.5 M trifluoromethanesulfonic acid in ether (4.9 mL, 2.47 mmol). The reaction was stirred at -40°C for 1 hour. After reaction, Et₃N was added into the solution to quench the acid. The solution was warmed to room temperature and filtered through celite. The filtrate was then quenched with 20% Na2S203(_aq) and washed with saturated NaHC03(_aq) ^and brine. The organic layer was dried over MgSC>4 and concentrated. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/10) to give compound 7 (86%, 4.33 g)

[0303] *H NMR (600 MHz, CDC1₃) δ 7.43 (d, J= 7.5 Hz, 2H), 7.39 - 7.24 (m, 8H), 4.87 (d, J = 11.9 Hz, 1H), 4.77 - 4.69 (m, 3H), 4.66 (d, J = 11.9 Hz, 1H), 4.52 (d, J= 2.8 Hz, 1H), 4.22 (dd, J= 12.5, 2.0 Hz, 1H), 4.09 (dd, J = 12.5, 1.6 Hz, 1H), 3.98 (dd, J = 10.0, 3.7 Hz, 1H), 3.82 (dd, J= 10.0, 3.0 Hz, 1H), 3.64 - 3.56 (m, 2H), 3.43 (dt, J= 10.0, 6.4 Hz, 1H), 3.23 (t, J = 6.9 Hz, 2H), 1.68 - 1.55 (m, 4H), 1.47 - 1.36 (m, 2H), 1.06 (s, 9H), 1.00 (s, 9H). ¹³C NMR (150 MHz, CDC1₃) δ 139.04, 138.73, 128.31, 128.17, 127.64, 127.60, 127.43, 98.04, 77.70, 74.37, 73.62, 71.17, 71.00, 67.86, 67.26, 51.33, 28.94, 28.67, 27.66, 27.31, 23.46, 23.43, 20.66. HRMS (ESI- TOF) calcd. for C₃₃H_{49 3}0₆Si a [M+Na]⁺: 634.3283, found: 634.3245.

2,3-0-di-benzyl-l-0-(5-azidopenty)-a-D-galactopyranoside (8)

[0304] Compound 7 (4 g, 6.53 mmol) was dissolved in THF (40 mL), followed by adding 1M tetra-H-butylammonium fluoride (19.59 mL, 19,59 mmol). The reaction was stirred at room temperature overnight. The solvent was then removed under reduced pressure. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/1) to give compound 8 (2.46 g, 81%): ^XH NMR (600 MHz, CDCI3) δ 7.39 - 7.27 (m, 10H), 4.84 - 4.78 (m, 3H), 4.70 (d, J= 11.5 Hz, 1H), 4.64 (d, J = 12.1 Hz, 1H), 4.08 (d, J = 2.6 Hz, 1H), 3.93 - 3.87 (m, 2H), 3.85 (dd, J= 9.8, 3.5 Hz, 1H), 3.82 - 3.74 (m, 2H), 3.66 (dt, J= 9.9, 6.7 Hz, 1H), 3.42 (dt, = 9.9, 6.4 Hz, 1H), 3.25 (t, J= 6.9 Hz, 2H), 1.71 - 1.58 (m, 4H), 1.50 - 1.39 (m, 2H). ¹³C NMR (150 MHz, CDCI₃) δ 138.44, 138.07, 128.54, 128.41, 127.96, 127.88, 127.82, 97.41, 77.38, 75.80, 73.31, 72.89, 69.10, 69.06, 67.94, 63.08, 51.33, 28.93, 28.66, 23.47. HRMS (ESI-TOF) calcd. for C₃₃H₄₉N₃0₆SiNa [M+Naf: 494.2262, found: 494.2273.

2,3,4-0-tri-benzyl-l-0-(5-azidopenty)-a-D-galactopyranoside (9)

[0305] A mixture of compound 8 (3 g, 6.36 mmol) and camphorsulfonic acid (1.47 g, 6.36 mmol) was dissolved in DMF (30 mL), followed by adding benzaldehyde dimethyl acetal (1.93 mL, 12.72 mmol). The reaction was stirred at room temperature for 3 hours. After reaction, the solution was diluted with EtOAc and washed with saturated NaHC0_3(aq) twice and brine. The organic layer was dried over MgSC>4 and concentrated. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/4). Purified compound (0.5 g, 0.89 mmol) was dissolved in CH₂C1₂ (0.5 mL) and cooled to 0°C, followed by sequentially adding 1M borane^■ THF (3.57 mL, 3.57 mmol) and trimethylsilyl trifluoromethanesulfonate (80 \\L, 0.36 mmol). The reaction was stirred at 0°C for 3 hours. Et₃N and MeOH were then added into the solution to quench the reaction. (Notice: To avoid the explosive bubble, MeOH must be added slowly into the solution until the bubble no longer produced.) The solvent was removed under reduced pressure. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/4) to give compound 9 (0.36 g, 72%): ^JH NMR (600 MHz, CDC1₃) δ 7.44 - 7.26 (m, 15H), 4.97 (d, J= 11.6 Hz, lH), 4.89 (d, J= 11.7 Hz, 1H), 4.83 (dd, J= 7.9, 4.1 Hz, 2H), 4.76 (d, J = 11.7 Hz, 1H), 4.67 (d, J = 12.0 Hz, 1H), 4.64 (d, J = 11.6 Hz, 1H), 4.05 (dd, J= 10.1, 3.7 Hz, 2H), 3.95 (dd, J= 10.1, 2.9 Hz, 1H), 3.90 (d, J= 1.8 Hz, 1H), 3.75 (t, J= 6.0 Hz, 1H), 3.70 (dd, J= 11.2, 6.5 Hz, 1H), 3.63 (dt, J= 9.8, 6.8 Hz, 1H), 3.48 (dd, J= 1 1.2, 5.1 Hz, 1H), 3.42 (dt, J = 9.9, 6.4 Hz, 1H), 3.23 (t, J= 6.9 Hz, 2H), 1.69 - 1.57 (m, 4H), 1.47 - 1.37 (m, 2H). ¹³C NMR (150 MHz, CDCI3) δ 138.78, 138.64, 138.20, 128.64, 128.51, 128.45, 128.37, 128.03, 127.93, 127.72, 127.61, 127.55, 97.63, 79.11, 76.69, 74.98, 74.47, 73.53, 73.38, 70.39, 67.92, 62.46, 51.35, 28.95, 28.66, 23.49. HRMS (ESI-TOF) calcd. for

[M+Na : 584.2731 , found: 584.2741.

Methyl-(5-acetamino-9-0-feri-butyldipheny]silyl-7,8-0-isopropylidene-5-/V,4-i?-carbony]- 3,5-dideoxy-2-0-(p-methy]phenyl-2,3,4-tri-i?-benzy]-l-deoxy-l-thio-p-D-glucopyranoside)- D-glycero-a-D-galacto-non-2-ulopyranoside)onate (10)

[0306] A mixture of compound 3 and 4 (1 g, 1.27 mmol, α/β mixture), compound 5 (0.47 g, 0.85 mmol), and activated pulverized MS-4A in dry CH₂CI₂ (10 mL) was stirred at room temperature for 3 hours. The solution was cooled to -70°C and followed by adding trimethylsilyl trifluoromethanesulfonate (336 μί, 1.52 mmol). The reaction was stirred at -70 °C for 1 hour. Et₃N was added into the solution to quench reaction. The solution was filtered through celite. The filtrate was washed with saturated aHC0_3(aq) and brine. The organic layer was then dried over MgSC>4 and concentrated. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/8) to give compound 10 (898 mg, 91%, a form): ^XH NMR (600 MHz, CDC1₃) δ 7.75 - 7.64 (m, 4H), 7.47 - 7.22 (m, 23H), 6.99 (d, J= 8.0 Hz, 2H), 4.98 (d, J = 11.4 Hz, 1H), 4.94 (d, J= 7.0 Hz, 1H), 4.80 (d, J = 10.2 Hz, 1H), 4.75 - 4.68 (m, 3H), 4.63 (d, J = 11.4 Hz, 1H), 4.54 (d, J = 9.7 Hz, 1H), 4.41 (q, J = 6.3 Hz, 1H), 4.24 - 4.14 (m, 3H), 4.11 - 4.04 (m, 1H), 3.95 - 3.82 (m, 4H), 3.57 (dd, J = 9.2, 2.8 Hz, 1H), 3.50 - 3.44 (m, 2H), 3.30 (s, 3H), 2.84 (dd, J= 12.0, 3.2 Hz, 1H), 2.50 (s, 3H), 2.27 (s, 3H), 1.97 (t, J= 12.5 Hz, 1H), 1.37 (s, 3H), 1.36 (s, 3H), 1.02 (s, 9H). ¹³C NMR (1 0 MHz, CDCb) δ 172.52, 168.56, 154.11, 139.05, 138.37, 138.30, 137.00, 135.65, 133.74, 133.37, 131.83, 130.66, 129.74, 129.59, 128.39, 128.31, 128.12, 127.84, 127.68, 127.62, 127.43, 127.23, 109.40, 98.88, 88.55, 83.99, 78.04, 77.44, 77.1 1 , 76.56, 76.16, 75.61, 74.57, 74.18, 73.04, 72.63, 62.32, 62.13, 59.69, 52.55, 36.86, 26.80, 26.07, 25.74, 25.12, 21.10, 19.16. HRMS (ESI-TOF) calcd. for QeHvsNOwSSiNa [M+Na : 1188.4570, found: 1 188.4586.

Methyl-(5-acetamino-9-0-½ri-butyldipheny]silyl-7,8-0-isopropylidene-5-/V,4-i>-carbony]- 3,5-dideoxy-2-0-(2,3,4-0-tri-benzyl-l-0-(5-azidopenty)-a-D-galactopyranoside)-D-glycero- a-D-galacto-non-2-ulopyranoside)onate (11)

[0307] A mixture of compound 3 and 4 (1 g, 1.26 mmol, α/β mixture), compound 9 (0.47 g, 0.84 mmol), and activated pulverized MS-4A in dry CH2CI2 (10 mL) was stirred at room temperature for 3 hours. The solution was cooled to -70°C, followed by adding trimethylsilyl

trifluoromethanesulfonate (335 μί, 1.51mmol). The reaction was stirred at -70°C for 1 hour. Et₃N was added into the solution to quench reaction. The solution was filtered through celite. The filtrate was washed with saturated NaHC0_3(aq) and brine. The organic layer was then dried over MgSC>4 and concentrated. The residue was purified by silica gel column chromatography

(EtOAc/hexane = 1/8) to give compound 11 (872 mg, 91%): ^JH NMR (600 MHz, CDC1₃) δ 7.77 - 7.57 (m, 4H), 7.43 - 7.16 (m, 21H), 5.00 - 4.93 (m, 2H), 4.85 - 4.76 (m, 1H), 4.74 (d, J= 11.7 Hz, 1H), 4.65 (d, J = 12.0 Hz, 1H), 4.57 (d, J = 11.2 Hz, 1H), 4.37 (q, J= 6.3 Hz, 1H), 4.29 - 4.19 (m, 2H), 4.13 (dd, J = 10.8, 6.2 Hz, 1H), 4.02 (dd, J= 9.6, 3.7 Hz, 1H), 3.95 - 3.90 (m, 3H), 3.89 - 3.81 (m, 3H), 3.61 (dt, J= 9.9, 6.7 Hz, 1H), 3.41 (t, J= 8.3 Hz, 1H), 3.37 - 3.29 (m, 4H), 3.20 (t, J= 6.9 Hz, 2H), 2.87 (dd, J= 1 1.9, 3.3 Hz, 1H), 2.50 (s, 3H), 1.97 (dd, J= 13.3, 12.0 Hz, 1H), 1.64 - 1.54 (m, 6H), 1.44 (s, 3H), 1.43 - 1.35 (m, 5H), 1.03 (s, 9H). ^1SC NMR (150 MHz, CDCI₃) δ 172.46, 168.52, 154.1 1 , 139.07, 138.88, 138.70, 135.63, 133.73, 133.53, 129.73, 129.64, 128.31, 128.15, 127.87, 127.76, 127.67, 127.63, 127.54, 127.41, 127.35, 127.25, 109.32, 99.18, 97.49, 78.89, 77.96, 76.72, 76.55, 74.67, 74.47, 74.42, 73.25, 73.04, 68.32, 67.84, 62.65, 62.38, 59.71 , 52.59, 51.30, 36.81, 28.86, 28.70, 26.83, 26.20, 25.84, 25.14, 23.39, 19.24. HRMS (ESI-TOF) calcd. for

[M+Na]⁺: 1193.5125, found: 1193.5151.

Methyl-(5-acetamino-9-0-¾i"i-butyldipheny]silyl-7,8-0-isopropylidene-3,5-dideoxy-2-0- (2,3,4-0-tri-benzy]-l-0-(5-azidopenty)-a-D-galactopyranoside)-D-g]ycero-a-D-galacto-non- 2-ulopyranoside)onate (12)

[0308] Compound 11 (1 g, 0.85 mmol) was dissolved in CH₂Cl₂/MeOH (10 mL, 1 :2), followed by adding 0.5M NaOMe in MeOH (1.7 mL, 0.85 mmol). The reaction was stirred at room temperature for 1 hour. IR-120 resin was then added into the solution to quench the reaction. The solvent was removed under reduced pressure, and the residue was purified by silica gel column chromatography (EtOAc/hexane = 2/1) to give compound 12 (760 mg, 78%): *H NMR

(600 MHz, CDCI₃) 87.73 - 7.63 (m, 4H), 7.46 - 7.19 (m, 21H), 5.34 (d, J= 8.0 Hz, 1H), 4.93 (d, J= 11.3 Hz, 1H), 4.84 - 4.77 (m, 2H), 4.76 (d, J= 3.6 Hz, 1H), 4.72 (d, J= 11.7 Hz, 1H), 4.64 (d, J= 12.0 Hz, 1H), 4.58 (d, J= 11.3 Hz, 1H), 4.34 - 4.25 (m, 2H), 4.14 - 4.08 (m, 2H), 4.00 (dd, J= 10.0, 3.6 Hz, 1H), 3.95 - 3.89 (m, 3H), 3.87 - 3.78 (m, 3H), 3.61 - 3.54 (m, 2H), 3.44 - 3.37 (m, 4H), 3.36 - 3.29 (m, 2H), 3.19 (t, J= 7.0 Hz, 2H), 2.62 (dd, J= 12.8, 4.4 Hz, 1H), 1.97 (s, 3H), 1.69 (t, J = 12.4 Hz, 1H), 1.63 - 1.52 (m, 4H), 1.43 - 1.34 (m, 5H), 1.30 (s, 3H), 1.06 (s, 9H). ¹³C NMR (150 MHz, CDCl₃) δ 172.10, 168.65, 139.03, 138.91, 138.74, 135.62, 135.58, 133.53, 133.47, 129.88, 129.79, 128.30, 128.15, 127.88, 127.82, 127.76, 127.70, 127.59, 127.37, 127.26, 109.57, 98.79, 97.49, 78.83, 77.47, 76.55, 75.12, 74.59, 74.47, 73.24, 72.97, 72.58, 69.42, 68.64, 67.78, 62.62, 62.10, 53.90, 52.20, 51.30, 40.51, 28.85, 28.67, 26.94, 26.26, 25.83, 23.42, 23.38, 19.33. HRMS (ESI-TOF) calcd. for CssH^OnSiNa [M+Na]⁺: 1167.5333, found: 1 167.5366.

5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-azidopenty)-a-D-galactopyranoside)-D-glycero-a- D-galacto-non-2-ulopyranosylonic acid (13)

[0309] Compound 12 (100 mg, 0.087 mmol) was dissolved in acetonitrile (2 ml) and cooled to 0°C, followed by adding 48% BF₃ · OEt₂ (336 μΐ,, 1.305 mmol). The reaction was stirred at 0°C for 3 hours. The solution was washed with saturated NaHC(¾(aq) and brine. The organic layer was then dried over M S0 and concentrated. The residue was dissolved in MeOH, and 2-3 drops of concentrated NaOH in MeOH were added. The reaction was stirred at room temperature overnight. The solvent was removed under reduced pressure. The residue was purified by reverse phase column (MeOH/H₂0 =1/1 to 4/1). The purified compound then proceeded through hydrogenation with PdOH as catalysis in MeOH/H₂0 under hi-pressure hydrogen at room temperature overnight. After reaction, the solution was filtered through celite and concentrated under reduced pressure. The residue was purified by Sephadex LH-20 to obtain compound 13 (31.4 mg, 65%, over three steps): *H NMR (600 MHz, D₂0) δ 4.94 (d, J =

3.5 Hz, 1H), 4.03 (dd, J = 8.2, 3.9 Hz, 1H), 4.01 - 3.98 (m, 1H), 3.94 - 3.87 (m, 3H), 3.87 - 3.80 (m, 3H), 3.78 - 3.59 (m, 6H), 3.56 (dt, J= 10.0, 6.2 Hz, 1H), 3.04 (t, J= 7.5 Hz, 2H), 2.74 (dd, J = 12.4, 4.7 Hz, 1H), 2.06 (s, 3H), 1.77 - 1.66 (m, 5H), 1.50 (dd, J = 15.3, 7.7 Hz, 2H). ¹³C NMR (150 MHz, D₂0) δ 175.00, 173.38, 100.28, 98.32, 72.51, 71.74, 69.47, 69.35, 69.26, 68.20, 68.18, 68.14, 68.00, 63.75, 62.58, 51.83, 40.12, 39.41, 28.02, 26.56, 22.36, 21.99. HRMS (ESI-TOF) calcd. for C₂₂H₄iN₂0i₄ [M+H]⁺: 557.2558, found: 557.2567.

Methyl-(5-acetamino-9-(5-½ri-butyldipheny]silyl-7,8-0-isopropylidene-5- V,4-i)-carbony]-2- (2,3,4-0-tri-benzyl-l-i)-(methy]-(5-acetamino-9-0-tert-butyldiphenylsilyl-7,8-0- isopropylidene-3,5-dideoxy-2-0-(2,3,4-0-tri-benzyl-l-0-(5-azidopenty)- -D- galactopyranoside)-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-D- galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate (14)

[0310] A mixture of compound 10 (458 mg, 0.39 mmol), compound 12 (300 mg, 0.26 mmol), and activated pulverized MS-4A in dry CH₂CI₂ (0.5 mL) was stirred at room temperature for 3 hours. The solution was cooled to 0 °C, and N-iodosuccinimide (105 mg, 0.47 mmol) was added. After that, the solution was cooled to -40 °C, followed by adding 0.5M trifluoromethanesulfonic acid in ether (0.52 mlL, 0.26 mmol). The reaction was stirred at -40 °C for 1 hour. After reaction, Et₃ was added into the solution to quench the reaction. The solution was filtered through celite. The filtrate was then quenched with 20% a₂S₂0_3(a(1) and wash with saturated NaHC(¾(aq) and brine. The organic layer was dried over MgSC>4 and concentrated. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/5 to 1/3) to give compound 14 (364 mg, 64%): *H NMR (600 MHz, CDC1₃) δ 7.70 - 7.62 (m, 8H), 7.43 - 7.15 (m, 42H), 5.91 (d, J = 7.5 Hz, 1H), 4.98 (d, J = 7.0 Hz, 1H), 4.90 (d, J = 11.1 Hz, 2H), 4.84 - 4.76 (m, 5H), 4.73 (d, J= 1 1.7 Hz, 1H), 4.70 - 4.60 (m, 4H), 4.52 (dd, J= 11.1 , 6.9 Hz, 2H), 4.39 (dd, J= 12.9, 6.6 Hz, 1H), 4.33 - 4.25 (m, 3H), 4.21 - 4.15 (m, 4H), 4.12 (d, J= 6.6 Hz, lH), 4.06 (dd, J= 10.6, 5.9 Hz, 1H), 4.01 (dd, J = 10.0, 3.6 Hz, 1H), 3.97 - 3.76 (m, 1 1H), 3.58 (dd, J = 16.6, 6.7 Hz, 1H), 3.46 (s, 3H), 3.46 - 3.40 (m, 1H), 3.40 - 3.36 (m, 1H), 3.34 - 3.26 (m, 4H), 3.17 (t, J= 7.0 Hz, 2H), 2.85 (d, J= 9.0 Hz, 1H), 2.77 (dd, J= 12.6, 4.5 Hz, 1H), 2.51 (s, 3H), 2.00 (t, J= 12.3 Hz, 1H), 1.68 (s, 3H), 1.58 - 1.52 (m, 5H), 1.44 (s, 3H), 1.41 (s, 3H), 1.39 (s, 3H), 1.39 - 1.34 (m, 2H), 1.32 - 1.25 (m, 3H), 1.02 (s, 18H). ¹³C NMR (150 MHz, CDC1₃) δ 172.50, 171.03, 168.60, 168.34, 153.99, 139.05, 138.97, 138.79, 138.77, 138.56, 138.50, 135.66, 135.62, 135.58, 133.83, 133.74, 133.47, 133.40, 129.86, 129.80, 129.66, 129.60, 128.33, 128.30, 128.24, 128.13, 127.91 , 127.87, 127.83, 127.76, 127.74, 127.71, 127.68, 127.58, 127.54, 127.48, 127.45, 127.40, 127.35, 127.32, 127.28, 109.48, 109.09, 99.11, 98.65, 97.45, 78.87, 78.72, 78.15, 77.90, 74.85, 74.77, 74.72, 74.67, 74.52, 74.10, 73.29, 73.20, 73.00, 72.85, 71.47, 69.35, 68.65, 67.75, 63.74, 62.65, 62.47, 61.61 , 59.65, 54.37, 52.81, 52.05, 51.30, 39.19,

36.19, 28.86, 28.68, 26.86, 26.82, 26.34, 25.98, 25.60, 25.12, 23.93, 23.54, 23.39, 21.07, 19.27,

19.20, HRMS (MALDI-TOF) calcd. for CmH^ sOzsSizNa [M+Na]⁺: 2208.9663, found: 2208.9660.

Methyl-(5-acetamino-9-0-½ri-butyldipheny]silyl-7,8-0-isopropylidene-2-(2,3,4-(?-tri- benzyl-l-0-(methyl-(5-acetamino-9-0-½rr-butyldiphenylsilyl-7,8-0-isopropy]idene-3,5- dideoxy-2-0-(2,3,4-i>-tri-benzyl-l-t>-(5-azidopenty)- -D-galactopyranoside)-D-glycero-a- D-galacto-non-2-ulopyranoside)onate)-a-D-galactopyranoside)-3,5-dideoxy-D-glycero-a-D- galacto-non-2-ulopyranoside)onate (15)

[0311] Compound 14 (100 mg, 46 μιηοΐ) was dissolved in CH₂Cl₂ MeOH (0.2 mL, 1 :2), followed by adding 0.5M NaOMe in MeOH (92 ocL, 46 μmol). The reaction was stirred at room temperature for 1 hour. IR- 120 resin was then added into the solution to quench the reaction. The solvent was removed under reduced pressure, and the residue was purified by silica gel column chromatography (EtOAc/hexane = 2/1) to give compound 15 (74.5 mg, 75%): 'H NMR (600 MHz, CDC1₃) δ 7.71 - 7.61 (m, 8H), 7.44 - 7.16 (m, 42H), 5.96 (d, J= 8.2 Hz, 1H), 5.72 (d, J= 7.8 Hz, 1H), 4.89 (dd, J= 22.6, 1 1.2 Hz, 2H), 4.84 - 4.70 (m, 7H), 4.69 - 4.62 (m, 2H), 4.52 (dd, J= 19.9, 1 1.2 Hz, 2H), 4.30 (dt, J= 12.5, 6.5 Hz, 2H), 4.22 - 4.1 1 (m, 5H), 4.06 - 3.90 (m, 9H), 3.88 - 3.69 (m, 8H), 3.66 - 3.56 (m, 2H), 3.47 (s, 3H), 3.45 - 3.39 (m, 2H), 3.35 (s, 3H), 3.33 - 3.27 (m, 3H), 3.17 (t, J= 7.0 Hz, 2H), 2.69 (dd, J= 12.7, 4.5 Hz, 1H), 2.51 (dd, J = 13.2, 4.5 Hz, 1H), 1.91 (s, 3H), 1.81 (s, 3H), 1.60 - 1.54 (m, 5H), 1.42 (s, 3H), 1.40 - 1.36 (m, 2H), 1.35 (s, 3H), 1.32 - 1.24 (m, 8H), 1.06 (s, 9H), 1.03 (s, 9H). ¹³C NMR (150 MHz, CDC1₃) δ 171.79, 170.96, 168.72, 168.58, 139.09, 138.95, 138.80, 138.77, 138.57, 138.44, 135.62, 135.59, 135.52, 133.82, 133.65, 133.28, 133.14, 130.00, 129.96, 129.71 , 129.62, 128.34, 128.30, 128.28, 128.16, 128.13, 127.87, 127.84, 127.78, 127.74, 127.70, 127.57, 127.50, 127.45, 127.42, 127.36, 127.32, 127.26, 109.27, 109.19, 98.77, 98.62, 97.45, 97.34, 78.84, 78.78, 77.87, 77.41 , 76.58, 76.36, 75.28, 75.09, 74.87, 74.70, 74.39, 73.51 , 73.44, 73.19, 73.05, 72.87, 72.34, 71.88, 69.81 , 69.19, 68.59, 67.74, 63.61 , 62.85, 62.68, 61.60, 54.62, 52.37, 52.12, 51.30, 39.16, 38.54, 28.85, 28.68, 26.97, 26.94, 26.86, 26.64, 26.29, 26.02, 25.41 , 23.57, 23.38, 23.34, 19.31, 19.24. HRMS (MALDI-TOF) calcd. for CmH^NsC^^Na [M+Na]⁺: 2182.9817, found: 2182.9830.

5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-i>-acetamino-3,5-dideoxy-2-i>-(l-i>-(5- aminopenty)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2-ulopyranosylonate)-a- D-galactopyranoside)-D-glycero-a-D-galacto-non-2-ulopyranosylonic acid (16)

[0312] Compound 15 (100 mg, 0.046 mmol) was dissolved in acetonitrile (2 mL) cooled to 0°C, followed by adding 48% BF3 · OEt₂ (178 μΐ., 0.69 mmol). The reaction was stirred at 0°C for 3 hours. The solution was washed with saturated aHC03(_aq) and brine. The organic layer was then dried over MgSC^ and concentrated. The residue was dissolved in MeOH, and 2-3 drops of concentrated NaOH in MeOH were added. The reaction was stirred at room temperature overnight. The solvent was removed under reduced pressure. The residue was purified by reverse phase column (MeOH/H₂0 =1/1 to 4/1). The purified compound then proceeded through hydrogenation with PdOH as catalysis in MeOH/H₂0 under hi-pressure hydrogen at room temperature overnight. After reaction, the solution was filtered through celite and concentrated under reduced pressure. The residue was purified by Sephadex LH-20 to obtain compound 16

(23.2 mg, 50%, over three steps): ^JH NMR (600 MHz, D₂0) δ 5.09 (d, J= 3.8 Hz, 1H), 4.93 (d, J= 3.5 Hz, 1H), 4.09 - 3.96 (m, 4H), 3.94 - 3.61 (m, 21H), 3.60 - 3.54 (m, 2H), 3.03 (t, J = 7.5 Hz, 2H), 2.90 (dd, J= 12.4, 4.6 Hz, 1H), 2.74 (dd, J= 12.4, 4.7 Hz, 1H), 2.09 (s, 3H), 2.04 (s, 3H), 1.77 - 1.60 (m, 6H), 1.51 - 1.40 (m, 2H). ¹³C NMR (150 MHz, D₂0) δ 174.97, 174.48, 173.45, 172.96, 100.29, 100.08, 98.37, 94.26, 72.45, 72.34, 72.07, 71.88, 71.86, 69.58, 69.55, 69.37, 69.35, 69.23, 68.89, 68.35, 68.27, 68.15, 68.07, 67.87, 63.92, 62.68, 62.61, 51.79, 49.43, 40.09, 39.43, 36.36, 28.06, 26.41, 22.40, 22.37, 22.01. HRMS (ESI-TOF) calcd. for

C₃9H₆₆N₃027 [M-H] : 1008.3884, found: 1008.3878.

5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-i?-acetamino-3,5-dideoxy-2-t>-(l-6⁾-(5-(3- mercapatopropanamido)penty)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonate)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonic acid (33)

[0313] Compound 16 (5 mg, 0.005 mmol) and 3,3 ^'-Dithiobis(sulfosuccinimidylpropionate) (DTSSP) (11 mg, 0.0018 mmol) were dissolved in pH 7.4 PBS buffer (1 mL) at r.t. overnight. At the beginning of the reaction, the solution was adjusted by IN NaOH(aq) and IN HCl to keep the pH value around 7 every 20 mins for three times. After reaction, dithiothreitol (DTT) (6.9 mg, 0.045 mol) was added into the solution and the solution was stirred at 40°C for another two hours. Compound 33 was purified through Sephadex LH-20 column chromatography: H NMR (600 MHz, D₂0) δ 5.08 (d, J= 3.7 Hz, 1H), 4.92 (d, J= 3.6 Hz, 1H), 4.09 - 3.50 (m, 27H), 3.23 (dd, J= 12.0, 6.5 Hz, 2H), 2.98 (t, J= 6.8 Hz, 1H), 2.91 (d, J= 8.7 Hz, 1H), 2.75 (dd, J= 12.3, 4.5 Hz, 1H), 2.68 (t, J= 6.8 Hz, 1H), 2.09 (s, 3H), 2.05 (s, 3H), 1.75 - 1.54 (m, 8H), 1.43 (dd, J = 15.1 , 7.5 Hz, 2H). °C NMR (150 MHz, D_zO) δ 174.98, 174.50, 174.13, 173.49, 172.97, 100.30, 100.11, 98.30, 94.47, 72.51, 72.47, 72.07, 71.89, 71.85, 69.54, 69.44, 69.35, 69.31 , 69.24, 68.90, 68.39, 68.29, 68.21, 68.14, 67.87, 63.82, 62.62, 51.80, 49.49, 40.09, 39.41, 39.27, 36.62, 28.22, 28.00, 22.79, 22.43, 22.01, 20.03.

Methyl-(5-acetamino-9-0-^-butyldiphenylsilyl-7,8-0-isopropylidene-5-^V,4-0-carbony]-2- (2,3,4-0-tri-benzy]-l-0-(methy]-(5-acetamino-9-0-½rt-butyldiphenylsilyl-7,8-0- isopropylidene-5-iV,4-0-carbonyl-2-(2,3,4-i?-tri-benzyl-l-i>-(Methyl-(5-acetamino-9-0-teri- butyldiphenylsilyl-7,8-0-isopropylidene-3,5-dideoxy-2-0-(2,3,4-6>-tri-benzyl-l-0-(5- azidopenty)-a-D-galactopyranoside)-D-g]ycero-a-D-galacto-non-2-ulopyranoside)onate)- - D-galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-D- galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate (17)

[0314] A mixture of compound 10 (80 mg, 69 μπιοΐ), compound 15 (100 mg, 46 μιηοΐ), and activated pulverized MS-4A in dry CH₂CI₂ (0.2 mL) was stirred at room temperature for 3 hours. The solution was cooled to 0°C, and N-iodosuccinimide (19 mg, 82.8 μηιοΐ) was added. Then, the solution was cooled to -40°C, followed by adding 0.5M trifluoromethanesulfonic acid in ether (92 μί, 46 μηιοΐ). The reaction was stirred at -40°C for 1 hour. Et₃N was added into the solution to quench the reaction. The solution was filter through celite. The filtrate was then quenched with 20% Na₂S₂0_3(aq and wash with saturated aHC0_3(aq) and brine. The organic layer was dried over MgS04 and concentrated. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/5 to 2/5) to give compound 17 (76 mg , 52%): *H NMR (600 MHz, CDC1₃) δ 7.70 - 7.60 (m, 15H), 7.42 - 7.18 (m, 60H), 5.90 (d, J= 7.5 Hz, 1H), 5.73 (d, J= 7.7 Hz, 1H), 4.98 (d, J= 7.1 Hz, 1H), 4.93 - 4.87 (m, 3H), 4.84 - 4.72 (m, 10H), 4.71 - 4.61 (m, 8H), 4.54 - 4.47 (m, 3H), 4.39 (dd, J = 12.9, 6.5 Hz, 1H), 4.33 - 4.26 (m, 4H), 4.24 - 4.13 (m, 7H), 4.12 - 4.04 (m, 6H), 4.01 (dd, 7 = 10.0, 3.6 Hz, 1H), 3.97 - 3.77 (m, 19H), 3.58 (dt, J= 9.9, 6.7 Hz, 1H), 3.45 (s, 3H), 3.43 - 3.40 (m, 1H), 3.40 - 3.35 (m, 4H), 3.35 - 3.29 (m, 2H), 3.28 (s, 3H), 3.17 (t, J = 7.0 Hz, 2H), 2.85 (dd, J= 11.9, 2.9 Hz, 1H), 2.78 - 2.74 (m, 1H), 2.71 (dd, J= 12.6, 4.5 Hz, 1H), 2.51 (s, 3H), 2.00 (t, J = 12.4 Hz, 1H), 1.70 (s, 3H), 1.68 (s, 3H), 1.61 - 1.54 (m, 5H), 1.45 (s, 3H), 1.42 - 1.34 (m, 1 1H), 1.31 - 1.25 (m, 6H), 1.04 - 0.99 (m, 27H). ¹³C NMR (150 MHz, CDC1₃) δ 172.48, 171.10, 171.02, 168.56, 168.30, 168.21, 153.92, 139.04, 138.93, 138.85, 138.82, 138.75, 138.70, 138.56, 138.49, 138.41, 135.62, 135.58, 135.56, 135.53, 133.80, 133.70, 133.53, 133.41 , 133.35, 129.83, 129.75, 129.60, 129.55, 128.31, 128.26, 128.24, 128.21, 128.15, 128.08, 127.86, 127.83, 127.80, 127.73, 127.71, 127.69, 127.67, 127.66, 127.60, 127.55, 127.53, 127.44, 127.33, 127.29, 127.28, 127.22, 109.40, 109.11, 109.06, 99.08, 98.93, 98.81, 98.63, 98.57, 97.40, 78.84, 78.70, 78.62, 78.22, 78.00, 77.89, 76.55, 76.48, 76.43, 74.80, 74.74, 74.68, 74.61, 74.57, 74.48, 74.20, 74.12, 73.24, 73.15, 73.01, 72.94, 72.78, 71.59, 71.32, 69.40, 68.57, 67.70, 63.73, 62.68, 62.49, 62.41 , 61.71, 61.48, 59.60, 54.30, 54.08, 52.78, 52.26, 52.00, 51.27, 39.31, 38.42, 36.21, 28.82, 28.65, 26.83, 26.77, 26.51, 26.33, 26.29, 26.01, 25.64, 25.60, 25.09, 23.56, 23.48, 23.35, 19.22, 19.16. HRMS (MALDI-TOF) calcd. for Ci₈oH₂i₆N₆0₄iSi Na [M+Na]⁺: 3224.4202, found: 3224.4131.

Methyl-(5-acetamino-9-0-feri-butyldipheny]silyl-7,8-0-isopropylidene-2-(2,3,4-0-tri- benzyl-l-0-(methyl-(5-acetamino-9-0-teri-butyldiphenylsilyl-7,8-0-isopropy]idene-5-7V,4-

0-carbonyl-2-(2,3,4-0-tri-benzyl-l-0-(Methyl-(5-acetamino-9-0-teri-butyldiphenylsilyl-

7,8-0-isopropylidene-3,5-dideoxy-2-0-(2,3,4-0-tri-benzyl-l-0-(5-azidopenty)-a-D- galactopyranoside)-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-D- galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-D- galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate (18)

[0315] Compound 17 (200 mg, 62.4 μηαοΐ) was dissolved in CH₂Cl₂/MeOH (2 mL, 1 :2), followed by adding 0.5M NaOMe in MeOH (124.8 μΐ,, 62.4 μηιοΐ). The reaction was stirred at room temperature for 1 hour. IR-120 resin was then added into the solution to quench the reaction. The solvent was removed under reduced pressure, and the residue was purified by silica gel column chromatography (EtOAc/hexane = 2/1) to give compound 18 (154 mg, 78%): *H NMR (600 MHz, CDC1₃) δ 7.72 - 7.61 (m, 14H), 7.43 - 7.18 (m, 61H), 5.95 (d, J= 8.2 Hz, 1H), 5.73 (t, J= 7.4 Hz, 2H), 4.94 - 4.86 (m, 3H), 4.85 - 4.75 (m, 7H), 4.75 - 4.61 (m, 9H), 4.54 - 4.48 (m, 3H), 4.35 - 4.24 (m, 3H), 4.22 - 3.77 (m, 33H), 3.76 - 3.68 (m, 2H), 3.65 - 3.55 (m, 2H), 3.48 - 3.39 (m, 9H), 3.35 - 3.26 (m, 7H), 3.20 - 3.14 (m, 2H), 2.76 (dd, J= 12.6, 4.3 Hz, 1H), 2.64 (dd, J= 12.6, 4.4 Hz, 1H), 2.52 (dd, J= 13.2, 4.5 Hz, 1H), 1.91 (s, 3H), 1.82 (s, 3H), 1.68 (s, 3H), 1.62 - 1.51 (m, 5H), 1.40 (s, 6H), 1.39 - 1.36 (m, 2H), 1.35 (s, 3H), 1.27 (3s, 12H), 1.05 (s, 9H), 1.02 (2s, J = 3.9 Hz, 18H). ¹³C NMR (150 MHz, CDC1₃) δ 171.79, 171.12, 170.98, 168.70, 168.45, 168.35, 139.07, 138.96, 138.89, 138.85, 138.79, 138.75, 138.60, 138.49, 138.41, 135.62, 135.59, 135.55, 135.52, 133.84, 133.74, 133.49, 133.45, 133.27, 133.12, 130.01, 129.97, 129.83, 129.64, 129.59, 128.36, 128.28, 128.21, 128.15, 128.1 1, 127.84, 127.74, 127.71, 127.69, 127.56, 127.48, 127.35, 127.32, 127.25, 109.25, 109.23, 109.10, 98.93, 98.78, 98.67, 98.56, 97.44, 78.87, 78.83, 78.63, 78.25, 77.81, 77.42, 76.58, 76.53, 76.27, 75.33, 75.04, 74.89, 74.81 , 74.77, 74.71 , 74.67, 74.60, 74.39, 74.22, 73.57, 73.43, 73.19, 73.14, 73.09, 72.96, 72.81, 72.23, 71.87, 71.63, 69.92, 69.42, 69.24, 68.60, 67.73, 63.71, 62.85, 62.71, 62.59, 61.84, 61.50, 54.66, 54.09, 52.35, 52.03, 51.30, 39.32, 39.15, 37.78, 30.95, 28.86, 28.69, 26.94, 26.86, 26.81, 26.68, 26.51, 26.32, 26.04, 25.71, 25.42, 23.59, 23.55, 23.38, 23.33, 19.32, 19.26, 19.18. HRMS (MALDI-TOF) calcd. for

[M+Na]⁺: 3198.4409, found: 3198.4446.

5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-i?-acetamino-3,5-dideoxy-2-t>-(l-6⁾-(5-0- acetamino-3,5-dideoxy-2-0-(l-0-(5-aminopenty)-a-D-galactopyranoside)-D-glycero-a-D- galacto-non-2-ulopyranosylonate)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonate)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonic acid (19)

[0316] Compound 18 (100 mg, 0.031 mmol) was dissolved in acetonitnle (2 mL) and cooled to 0°C, followed by adding 48% BF₃ · OEt₂ (230 μί, 0.4720 mmol). The reaction was stirred at 0°C for 3 hours. The solution was washed with saturated NaHC03(_aq) and brine. The organic layer was then dried over MgSC and concentrated. The residue was dissolved in MeOH, and 2-3 drops of concentrated NaOH in MeOH were added. The reaction was stirred at room temperature overnight. The solvent was removed under reduced pressure. The residue was purified by reverse phase column (MeOH/H₂0 =1/1 to 4/1). The purified compound then proceeded through hydrogenation with PdOH as catalysis in MeOH/H₂0 under hi-pressure hydrogen at room temperature overnight. After reaction, the solution was filtered through celite and concentrated under reduced pressure. The residue was purified by Sephadex LH-20 to obtain compound 19 (20 mg, 45%, over three steps): ^XH NMR (600 MHz, D₂0) δ 5.10 (d, J = 3.8 Hz, 1H), 5.08 (d, J= 3.5 Hz, 1H), 4.93 (d, J= 3.4 Hz, 1H), 4.09 - 3.51 (m, 39H), 3.03 (t, J= 7.5 Hz, 2H), 2.93 - 2.87 (m, 2H), 2.74 (dd, J= 12.5, 4.4 Hz, 1H), 2.10 (2s, J= 11.8 Hz, 6H), 2.05 (s, 3H), 1.76 - 1.59 (m, 7H), 1.52 - 1.42 (m, 2H). ¹³C NMR (150 MHz, D₂0) δ 174.98, 174.51, 173.46, 173.01, 100.36, 100.18, 98.39, 98.26, 94.87, 94.26, 73.08, 72.45, 72.33, 72.09, 72.01 , 71.86, 71.82, 69.62, 69.55, 69.39, 69.36, 69.25, 69.07, 68.91 , 68.38, 68.29, 68.16, 68.10, 68.04, 67.89, 67.77, 63.91, 62.88, 62.61, 51.80, 49.47, 40.03, 39.43, 36.70, 36.63, 36.27, 28.08, 26.39, 22.46, 22.42, 22.38, 22.00. HRMS (ESI-TOF) calcd. for C56H92N4O40 [M-2H]^{2 "}:

730.2644, found: 730.2645

Methyl-(5-acetamino-9-0-teri-butyldipheny]silyl-7,8-0-isopropylidene-5-^V,4-0-carbony]-2-

(2,3,4-0-tri-benzy]-l-0-(methy]-(5-acetamino-9-0-tei'i-butyldiphenylsilyl-7,8-0- isopropylidene-5-A^r,4-0-carbonyl-2-(2,3,4-i?-tri-benzyl-l-i?-(methyl-(5-acetainino-9-i>-tei*i- butyldiphenylsilyl-7,8-0-isopropylidene-5- V,4-0-carbony]-2-(2,3,4-i>-tri-benzyl-l-0-

(Methyl-(5-acetamino-9-<?-tert-butyldiphenylsilyl-7,8-6>-isopropylidene-3,5-dideoxy-2-0-

(2,3,4-0-tri-benzy]-l-6>-(5-azidopenty)-a-D-galactopyranoside)-D-g]ycero-a-D-galacto-non-

2-ulopyranoside)onate)-a-D-galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2- ulopyranoside)onate)-a-D-galactopyranoside)-3,5-dideoxy-D-g]ycero-a-D-galacto-non-2- ulopyranoside)onate)-oi_^D-galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2- ulopyranoside)onate (20)

[0317] A mixture of compound 10 (65 mg, 56 μιτιοι), compound 18 (100 mg, 31 μσιοΐ), and activated pulverized MS-4A in dry CH₂CI₂ (0.2 mL) was stirred at room temperature for 3 hours. The solution was cooled to 0°C, and N-iodosuccinimide (15.5 mg, 67 μιηοΐ) was added. After that, the solution was cooled to -40 °C, followed by adding 0.5M trifluoromethanesulfonic acid in ether (62 μΐ,, 31 μηιοΐ). The reaction was stirred at -40°C for 1 hour. The solution was filter through celite. The filtrate was then quenched with 20% Na₂S₂C>3(aq) and wash with saturated aHC0_3(aq) and brine. The organic layer was dried over MgSC>4 and concentrated. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/5 to 1/3) to give compound 20 (60 mg, 46%): ^XH NMR (600 MHz, CDC1₃) δ 7.70 - 7.60 (m, 18H), 7.41 - 7.19 (m, 82H), 5.89 (d, J = 7.5 Hz, 1H), 5.73 (t, J= 7.8 Hz, 2H), 4.98 (d, J= 7.1 Hz, 1H), 4.94 - 4.86 (m, 4H), 4.85 - 4.75 (m, 10H), 4.75 - 4.61 (m, 12H), 4.54 - 4.46 (m, 4H), 4.39 (dd, J= 13.0, 6.5 Hz, 1H), 4.33 - 4.04 (m, 24H), 4.01 (dd, J= 10.0, 3.5 Hz, 1H), 3.97 - 3.77 (m, 23H), 3.59 (dt, J = 10.1 , 6.6 Hz, 1H), 3.47 - 3.25 (m, 22H), 3.17 (t, J= 7.0 Hz, 2H), 2.84 (dd, J= 11.7, 2.7 Hz, 1H), 2.78 - 2.69 (m, 3H), 2.51 (s, 3H), 1.99 (t, J = 12.4 Hz, 1H), 1.69 (3s, 12H), 1.60 - 1.54 (m, 7H), 1.44 (s, 3H), 1.42 - 1.38 (m, 14H), 1.29 - 1.25 (m, 9H), 1.01 (4s, 36H). ¹³C NMR (150 MHz, CDCI₃) δ 207.00, 172.51, 171.14, 171.07, 171.04, 168.59, 168.33, 168.24, 168.18, 153.94, 139.07, 138.95, 138.89, 138.85, 138.82, 138.79, 138.71, 138.60, 138.53, 138.51, 138.44, 135.64, 135.61, 135.59, 135.56, 135.53, 133.83, 133.73, 133.55, 133.44, 133.42, 133.38, 129.86, 129.78, 129.63, 129.57, 128.34, 128.28, 128.25, 128.18, 128.17, 128.10, 127.87, 127.85, 127.83, 127.76, 127.72, 127.66, 127.58, 127.55, 127.51 , 127.48, 127.44, 127.36, 127.34, 127.31, 127.29, 127.24, 109.41, 109.16, 109.08, 99.11, 98.98, 98.80, 98.66, 98.62, 97.43, 78.87, 78.73, 78.68, 78.65, 78.25, 78.09, 78.06, 77.93, 76.57, 76.50, 76.43, 76.37, 74.75, 74.70, 74.64, 74.61, 74.59, 74.50, 74.28, 74.23, 74.11 , 73.26, 73.18, 73.07, 73.03, 72.96, 72.80, 71.61 , 71.53, 71.43, 69.53, 69.42, 68.59, 67.71, 63.75, 62.71, 62.55, 62.44, 61.73, 61.67, 61.49, 60.41 , 59.62, 54.20, 54.12, 53.94, 52.80, 52.28, 52.26, 52.02, 51.29, 39.35, 38.57, 38.49, 36.24, 31.59, 30.94, 29.71, 28.84, 28.68, 26.95, 26.86, 26.80, 26.58, 26.52, 26.36, 26.32, 26.05, 25.72, 25.68, 25.63, 25.11, 23.61, 23.57, 23.51 , 23.37, 22.66, 21.06, 19.25, 19.18, 19.17, 14.21 , 14.13. HRMS (ESI-TOF) calcd. for C238H287 7O54S14 [M+2H]²⁺: 2110.9538, found: 2110.9494.

Methyl-(5-acetamino-9-0-tert-butyldiphenylsilyl-7,8-0-isopropylidene-2-(2,3,4-0-tri- benzyl-l-6>-(methyl-(5-acetamino-9-0-teri-butyldiphenylsilyl-7,8-0-isopropylidene-5-JV,4- i?-carbony]-2-(2,3,4-i?-tri-benzyl-l-i?-(methyl-(5-acetamino-9-(?-teri-butyldiphenylsilyl-7,8-

0-isopropylidene-5-A^r,4-0-carbonyl-2-(2,3,4-0-tri-benzyl-l-0-(Methyl-(5-acetamino-9-0- teri-butyldiphenylsilyl-7,8-0-isopropylidene-3,5-dideoxy-2-0-(2,3,4-0-tri-benzy]-l-0-(5- azidopenty)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-

D-galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-D- galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-D- galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate (21)

[0318] Compound 20 (100 mg, 23 μηιοΐ) was dissolved in CH₂Cl₂/MeOH (0.2 mL, 1 :2), followed by adding 0.5M NaOMe in MeOH (46 μί, 23 μηιοΐ). The reaction was stirred at room temperature for 1 hour. IR-120 resin was then added into the solution to quench the reaction. The solvent was removed under reduced pressure, and the residue was purified by silica gel column chromatography (EtOAc/hexane = 2/1) to give compound 21 (67 mg, 70%): ¹H NMR (600 MHz, CDCI₃) δ 7.71 - 7.60 (m, 23H), 7.44 - 7.18 (m, 77H), 5.96 (d, J= 8.3 Hz, 1H), 5.77 - 5.70 (m, 3H), 4.94 - 4.86 (m, 5H), 4.85 - 4.76 (m, 12H), 4.74 - 4.61 (m, 15H), 4.54 - 4.46 (m, 5H), 4.34 - 4.23 (m, 6H), 4.22 - 3.76 (m, 54H), 3.74 - 3.64 (m, 5H), 3.59 (dt, J= 9.8, 6.7 Hz, 2H), 3.48 - 3.26 (m, 26H), 3.17 (t, J= 7.0 Hz, 2H), 2.76 (dd, J= 12.6, 4.2 Hz, 1H), 2.71 (dd, J = 12.3, 4.2 Hz, 1H), 2.65 (dd, J= 12.6, 4.2 Hz, 1H), 2.51 (dd, J= 13.2, 4.4 Hz, 1H), 1.90 (s, 3H), 1.83 (s, 3H), 1.69 (s, 3H), 1.68 (s, 3H), 1.64 - 1.54 (m, 5H), 1.42 - 1.39 (m, 9H), 1.38 - 1.36 (m, 2H), 1.35 (s, 3H), 1.29 - 1.25 (m, 12H), 1.06 (s, 9H), 1.02 (3s, 27H). ¹³C NMR (150 MHz, CDCI₃) δ 171.78, 171.13, 171.08, 170.97, 168.69, 168.40, 168.34, 168.25, 139.06, 138.94, 138.88, 138.84, 138.82, 138.78, 138.71 , 138.60, 138.54, 138.48, 138.38, 135.60, 135.58, 135.52, 133.83, 133.72, 133.54, 133.46, 133.42, 133.25, 133.11, 130.00, 129.96, 129.82, 129.78, 129.62, 129.57, 128.35, 128.34, 128.28, 128.21 , 128.17, 128.14, 128.10, 127.83, 127.76, 127.73, 127.71, 127.66, 127.64, 127.58, 127.54, 127.49, 127.46, 127.44, 127.38, 127.32, 127.31, 127.24, 109.19, 109.15, 109.09, 99.04, 98.85, 98.77, 98.66, 98.64, 98.60, 97.42, 97.34, 78.85, 78.64, 78.24, 78.06, 77.85, 77.42, 76.56, 76.48, 76.38, 76.22, 75.32, 75.01, 74.77, 74.70, 74.63, 74.58, 74.37, 74.22, 74.14, 73.51 , 73.42, 73.18, 73.06, 73.03, 72.96, 72.80, 72.32, 71.84, 71.66, 71.53, 69.92, 69.50, 69.41, 69.21 , 68.59, 67.71, 63.73, 62.84, 62.71 , 62.55, 61.80, 61.69, 61.49, 54.66, 53.99, 53.92, 52.34, 52.27, 52.19, 52.02, 51.29, 39.27, 39.13, 38.54, 37.79, 30.94, 29.70, 29.06, 28.84, 28.67, 26.93, 26.86, 26.80, 26.67, 26.53, 26.51, 26.31 , 26.04, 25.71 , 25.39, 23.60, 23.54, 23.37, 23.31 , 19.30, 19.24, 19.16. HRMS (ESI-TOF) calcd. for C237H289N7O53S14 [M+2H]²⁺:

2097.9742, found: 2097.9598.

5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-0- acetamino-3,5-dideoxy-2-0-(l-i?-(5-i>-acetamino-3,5-dideoxy-2-i)-(l-i?-(5-aminopenty)-a-

D-galactopyranoside)-D-glycero-a-D-galacto-non-2-ulopyranosylonate)-a-D- galactopyranoside)-D-glycero-a-D-galacto-non-2-ulopyranosylonate)-a-D- galactopyranoside)-D-glycero-a-D-galacto-non-2-ulopyranosylonate)-a-D- galactopyranoside)-D-glycero-a-D-galacto-non-2-ulopyranosylonic acid (22)

[0319] Compound 21 (100 mg, 0.024 mmol) was dissolved in acetonitrile (2 mL) and cooled to 0 °C, followed by adding 48% BF₃ ^■ OEt₂ (174 μί, 0.358 mmol). The reaction was stirred at 0 °C for 3 hours. The solution was washed with saturated N HC03(_aq) and brine. The organic layer was then dried over MgS0₄ and concentrated. The residue was dissolved in MeOH, and 2~3 drops of concentrated NaOH in MeOH were added. The reaction was stirred at room

temperature overnight. The solvent was removed under reduced pressure. The residue was purified by reverse phase column (MeOH/H₂0 = 1/1 to 4/1). The purified compound then proceeded through hydrogenation with PdOH as catalysis in MeOH/H₂0 under hi-pressure hydrogen at room temperature overnight. After reaction, the solution was filtered through celite and concentrated under reduced pressure. The residue was purified by Sephadex LH-20 to obtain compound 22 (18 mg, 40%, over three steps): ^XH NMR (600 MHz, D₂0) δ 5.10 (d, J = 3.8 Hz, 1H), 5.07 (d, J= 3.4 Hz, 2H), 4.93 (d, J= 3.6 Hz, 1H), 4.08 - 3.53 (m, 54H), 3.03 (t, J= 7.5 Hz, 2H), 2.93 - 2.86 (m, 3H), 2.74 (dd, J= 12.4, 4.7 Hz, 1H), 2.09 (3s, J= 1.8 Hz, 9H), 2.04 (s, 3H), 1.76 - 1.62 (m, 8H), 1.48 (p, J= 7.6 Hz, 2H). "C NMR (150 MHz, D₂0) δ 174.96, 174.50, 174.46, 173.47, 173.04, 173.02, 100.33, 100.22, 100.18, 100.13, 98.38, 98.25, 95.01, 94.87, 94.25, 73.25, 73.10, 72.44, 72.32, 72.09, 71.96, 71.87, 71.81 , 69.61, 69.54, 69.38, 69.34, 69.24, 69.08, 69.03, 68.90, 68.38, 68.28, 68.16, 68.09, 68.03, 67.88, 67.79, 67.75, 63.89, 62.90, 62.80, 62.60, 51.79, 49.47, 40.03, 39.44, 36.80, 36.73, 36.64, 36.27, 28.08, 26.44, 22.49, 22.46, 22.41 , 22.38, 22.00. HRMS (ESI-TOF) calcd. for C₇3Hii_{9 5}053 [M-2H]^2": 956.8385, found: 956.8393.

5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-0- acetamino-3,5-dideoxy-2-0-(l-0-(5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-(3- mercapatopropanamido)penty)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonate)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonate)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonate)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonic acid (35)

[0320] Compound 22 (5 mg, 0.002 mmol) and 3,3 ^'-Dithiobis(sulfosuccinimidylpropionate) (DTSSP) (6.0 mg, 0.010 mmol) were dissolved in pH 7.4 PBS buffer (1 mL) at r.t. overnight. At the beginning of the reaction, the solution was adjusted by IN NaOH(aq) and IN HC1 to keep the pH value around 7 every 20mins for three times. After reaction, dithiothreitol (DTT) (3.8 mg, 0.025 mol) was added into the solution, and the solution was stirred at 40 °C for another two hours. Compound 35 was purified through Sephadex LH-20 column chromatography: H NMR (600 MHz, D₂0) 5 5.10 - 5.04 (m, 3H), 4.92 (d, J= 4.0 Hz, 1H), 4.08 - 3.52 (m, 54H), 3.27 - 3.22 (m, 2H), 2.93 - 2.86 (m, 3H), 2.80 (t, J = 6.6 Hz, 1H), 2.74 (dd, J= 12 A, 4.6 Hz, 1H), 2.56 (t, J = 6.9 Hz, 1H), 2.09 (2s, J = 1.9 Hz, 9H), 2.04 (s, 3H), 1.75 - 1.54 (m, 10H), 1.47 - 1.40 (m, 2H). ¹³C NMR (150 MHz, D₂0) δ 174.97, 174.51, 174.13, 173.48, 173.07, 100.24, 100.14, 98.30, 95.07, 94.89, 94.59, 73.12, 72.61, 72.45, 72.08, 71.96, 71.86, 71.82, 69.61, 69.45, 69.30, 69.21 , 69.08, 68.91 , 68.39, 68.21, 68.08, 67.77, 63.82, 62.79, 62.61 , 51.80, 49.48, 40.04, 39.40, 39.26, 36.64, 28.22, 27.99, 22.79, 22.47, 22.01, 20.02.

Methyl-(5-acetamino-9-0-fert-butyldiphenylsilyl-7,8-0-isopropylidene-5-A^r,4-i?-carbony]-2-

(2,3,4-0-tri-benzy]-l-i)-(methy]-(5-acetamino-9-0-tert-butyldiphenylsilyl-7,8-0- isopropylidene-5-iV,4-0-carbonyl-2-(2,3,4-i?-tri-benzyl-l-i?-(methyl-(5-acetamino-9-0-tei"i- butyldiphenylsilyl-7,8-0-isopropylidene-5- V,4-0-carbony]-2-(2,3,4-0-tri-benzyl-l-0-

(methyl-(5-acetamino-9-0-tefi-butyldiphenylsilyl-7,8-i?-isopropylidene-5-iV,4-0-carbonyl-

2-(2,3,4-0-tri-benzy]-l-0-(Methyl-(5-acetamino-9-6>-fei-i-butyldiphenylsilyl-7,8-0- isopropylidene-3,5-dideoxy-2-0-(2,3,4-0-tri-benzyl-l-C>-(5-azidopenty)-a-D- galactopyranoside)-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-D- galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-D- galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-D- galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate)-a-D- galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2-ulopyranoside)onate (23)

[0321] A mixture of compound 10 (56 mg, 48 μηιοΐ), compound 21 (100 mg, 24 μιηοΐ), and activated pulverized MS-4A in dry CH₂CI₂ (0.2 mL) was stirred at room temperature for 3 hours. The solution was cooled to 0 °C, and N-iodosuccinimide (13 mg, 60 μηιοΐ) was added. Then, the solution was cooled to -40 °C, followed by adding 0.5 M trifluoromethanesulfonic acid in ether (48 μί, 24 μιηοΐ). The reaction was stirred at -40 °C for 1 hour. After reaction, the solution was filter through celite. The filtrate was then quenched with 20% Na₂S₂0_3(aq) and wash with saturated NaHCC>3(_aq) and brine. The organic layer was dried over MgSC>4 and

concentrated. The residue was purified by silica gel column chromatography (EtOAc/hexane = 1/5 to 1/3) to give compound 23 (43 mg, 35%): ^XH NMR (600 MHz, CDC1₃) δ 7.70 - 7.58 (m, 22H), 7.40 - 7.14 (m, 103H), 5.88 (d, J= 6.6 Hz, 1H), 5.77 - 5.68 (m, 3H), 5.00 - 4.86 (m, 7H), 4.85 - 4.75 (m, 12H), 4.75 - 4.60 (m, 15H), 4.55 - 4.45 (m, 5H), 4.38 (d, J= 6.5 Hz, 1H), 4.32 - 4.23 (m, 7H), 4.21 - 4.03 (m, 22H), 3.98 - 3.77 (m, 29H), 3.68 - 3.56 (m, 3H), 3.48 - 3.25 (m, 27H), 3.17 (t, J = 7.0 Hz, 2H), 2.83 (d, J= 9.3 Hz, 1H), 2.78 - 2.67 (m, 4H), 2. 1 (s, 3H), 2.03 - 1.96 (m, 1H), 1.70 (2s, J= 14.6 Hz, 12H), 1.45 - 1.35 (m, 22H), 1.31 - 1.23 (m, 18H), 1.04 - 0.99 (m, 45H). ¹³C NMR (150 MHz, CDCI3) δ 172.53, 171.14, 171.07, 171.03, 168.61, 168.36, 168.28, 168.23, 153.94, 139.08, 138.95, 138.89, 138.85, 138.82, 138.80, 138.70, 138.61, 138.54, 138.53, 138.50, 138.44, 135.62, 135.57, 135.53, 133.84, 133.73, 133.53, 133.44, 133.38, 129.87, 129.79, 129.64, 129.58, 128.36, 128.34, 128.31 , 128.29, 128.27, 128.26, 128.18, 128.11, 127.86, 127.83, 127.74, 127.69, 127.60, 127.55, 127.52, 127.45, 127.38, 127.34, 127.32, 127.25, 109.39, 109.17, 109.11, 109.09, 109.03, 99.14, 98.74, 98.67, 97.43, 78.86, 78.74, 78.70, 78.65, 78.42, 78.25, 78.10, 78.04, 77.93, 76.58, 76.48, 76.39, 76.31 , 76.27, 74.85, 74.75, 74.71, 74.60, 74.56, 74.48, 74.12, 74.03, 73.29, 73.18, 73.09, 73.07, 72.97, 72.81, 71.78, 71.70, 71.55, 69.56, 69.47, 69.44, 68.60, 67.72, 63.85, 62.73, 62.56, 62.46, 61.90, 61.82, 61.51 , 59.63, 53.91, 53.63, 53.45, 52.81 , 52.29, 52.27, 52.03, 51.30, 39.24, 38.34, 36.23, 29.71, 28.85, 28.68, 26.98, 26.95, 26.87, 26.81 , 26.61, 26.58, 26.53, 26.37, 26.32, 26.05, 25.71 , 25.66, 25.61 , 25.12, 23.61, 23.58, 23.51, 23.38, 19.26, 19.19, 19.17. HRMS (ESI-TOF) calcd. For C₂96H35eN₈0₅₇Si₅ [M+2H]²⁺:

2617.1766, found: 2617.1804.

Methyl-(5-acetamino-9-0-teri-butyldipheny]silyl-7,8-0-isopropylidene-2-(2,3,4-(?-tri- benzyl-l-0-(methyl-(5-acetamino-9-0-teri-butyldiphenylsilyl-7,8-0-isopropy]idene-5- V,4-

0-carbony]-2-(2,3,4-0-tri-benzyl-l-t>-(methyl-(5-acetamino-9-0-½i'/-butyldiphenylsilyl-7,8-

0-isopropylidene-5-A^r,4-0-carbonyl-2-(2,3,4-0-tri-benzyl-l-i)-(methyl-(5-acetamino-9-i>- teri-butyldiphenylsilyl-7,8-0-isopropylidene-5-jV,4-0-carbonyl-2-(2,3,4-0-tri-benzyl-l-0-

(2,3,4-0-tri-benzyl-l-i)-(5-azidopenty)-a-D-galactopyranoside)-D-g]ycero-a-D-galacto-non-

2-ulopyranoside)onate)-a-D-galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2- ulopyranoside)onate)-a-D-galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2- ulopyranoside)onate)-o^D-galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2- ulopyranoside)onate)-a-D-galactopyranoside)-3,5-dideoxy-D-glycero-a-D-galacto-non-2- ulopyranoside)onate (24)

[0322] Compound 23 (100 mg, 19 μηιοΐ) was dissolved in CH₂Cl₂ MeOH (0.2 mL, 1 :2), followed by adding 0.5M NaOMe in MeOH (38 μί, 19 μηιοΐ). The reaction was stirred at room temperature for 1 hour. IR-120 resin was then added into the solution to quench the reaction. The solvent was removed under reduced pressure, and the residue was purified by silica gel column chromatography (EtOAc/hexane = 2/1) to give compound 24 (71 mg, 72%): ¹H NMR

(600 MHz, CDC1₃) δ 7.71 - 7.58 (m, 30H), 7.42 - 7.18 (m, 95H), 5.96 (d, J= 8.3 Hz, 1H), 5.78 - 5.69 (m, 4H), 4.94 - 4.86 (m, 7H), 4.84 - 4.76 (m, 15H), 4.75 - 4.61 (m, 20H), 4.53 - 4.45 (m, 6H), 4.33 - 4.24 (m, 7H), 4.21 - 3.99 (m, 34H), 3.98 - 3.90 (m, 13H), 3.89 - 3.77 (m, 24H), 3.74 - 3.65 (m, 5H), 3.58 (dt, J = 10.1, 6.7 Hz, 2H), 3.46 - 3.26 (m, 34H), 3.17 (t, J= 7.0 Hz, 2H), 2.75 (dd, J= 11.9, 4.6 Hz, 1H), 2.73 - 2.68 (m, 2H), 2.64 (dd, J = 12.2, 4.1 Hz, 1H), 2.50 (dd, J= 13.3, 4.3 Hz, 1H), 1.90 (s, 3H), 1.84 (s, 3H), 1.70 (s, 6H), 1.68 (s, 3H), 1.61 - 1.54 (m, 5H), 1.40 (2s, J = 5.9 Hz, 12H), 1.38 - 1.36 (m, 2H), 1.34 (s, 3H), 1.28 - 1.24 (m, 15H), 1.06 (s, 9H), 1.01 (2s, J= 3.4 Hz, 36H). ¹³C NMR (150 MHz, CDC1₃) δ 171.74, 171.13, 171.07, 170.94, 168.69, 168.41, 168.34, 168.26, 168.22, 139.07, 138.95, 138.85, 138.80, 138.70, 138.61, 138.54,

138.49, 138.37, 135.60, 135.52, 133.83, 133.73, 133.53, 133.43, 133.25, 133.11, 130.00, 129.96, 129.78, 129.62, 129.57, 128.34, 128.28, 128.21 , 128.17, 128.14, 128.10, 127.83, 127.73, 127.54,

127.50, 127.47, 127.43, 127.37, 127.31 , 109.16, 109.09, 98.92, 98.77, 98.70, 98.66, 97.42, 97.26, 78.85, 78.65, 78.24, 78.13, 78.04, 77.85, 76.56, 76.49, 76.33, 76.20, 75.32, 75.02, 74.73, 74.59, 74.36, 74.18, 73.43, 73.17, 73.04, 72.95, 72.80, 72.41, 71.82, 71.65, 69.93, 69.53, 69.42, 69.21 , 68.59, 67.71 , 63.77, 62.85, 62.72, 62.60, 61.85, 61.75, 61.50, 54.71, 54.02, 53.74, 52.34, 52.28, 52.02, 51.29, 39.29, 39.08, 38.44, 37.72, 29.71 , 28.84, 28.67, 26.93, 26.86, 26.81, 26.68, 26.55, 26.52, 26.31 , 26.04, 25.71, 25.39, 23.60, 23.57, 23.37, 23.31 , 19.31, 19.25, 19.16. HRMS (ESI-TOF) calcd. for C295H358N8O66S15 [M+2H]²⁺: 2604.1869, found: 2604.1754.

5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-0-acetamino-3,5-dideoxy-2-0-(l-0-(5-0- acetamino-3,5-dideoxy-2-i?-(l-i?-(5-i?-acetamino-3,5-dideoxy-2-i?-(l-0-(5-0-acetamino-

3,5-dideoxy-2-0-(l-t>-(5-aminopenty)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-

2-ulopyranosylonate)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonate)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonate)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonate)-a-D-galactopyranoside)-D-glycero-a-D-galacto-non-2- ulopyranosylonic acid (25)

[0323] Compound 24 (100 mg, 0.019 mmol) was dissolved in acetonitrile (2 mL) and cooled to 0 °C, followed by adding 48% BF₃ ^■ OEt₂ (130 ocL, 0.288 mmol). The reaction was stirred at 0 °C for 3 hours. The solution was washed with saturated NaHC0₃(_aq) and brine. The organic layer was then dried over MgS0₄ and concentrated. The residue was dissolved in MeOH, and 2-3 drops of concentrated NaOH in MeOH was added. The reaction was stirred at room temperature overnight. The solvent was removed under reduced pressure. The residue was purified by reverse phase column (MeOH/H₂0 =1/1 to 4/1). The purified compound then proceeded through hydrogenation with PdOH as catalysis in MeOH/H₂0 under hi-pressure hydrogen at room temperature overnight. After reaction, the solution was filtered through celite and concentrated under reduced pressure. The residue was purified by Sephadex LH-20 to obtain compound 25 (17 mg, 38%, over three steps): *H NMR (600 MHz, D₂0) δ 5.10 (d, J= 3.8 Hz, 1H), 5.08 (d, J = 2.5 Hz, 3H), 4.93 (d, J= 3.5 Hz, 1H), 4.09 - 3.54 (m, 76H), 3.03 (t, J= 7.5 Hz, 2H), 2.93 - 2.85 (m, 4H), 2.74 (dd, J = 12.3, 4.3 Hz, 1H), 2.09 (s, 14H), 2.05 (s, 3H), 1.77 - 1.63 (m, 9H), 1.52 - 1.45 (m, 1H). ¹³C NMR (150 MHz, D₂0) δ 175.00, 174.52, 173.48, 172.99, 100.32, 100.12, 98.48, 98.33, 94.39, 94.18, 72.56, 72.39, 72.17, 72.00, 71.75, 69.82, 69.56, 69.46, 69.19, 69.10, 68.74, 68.41 , 68.21, 68.04, 67.90, 62.85, 62.67, 62.41, 51.88, 49.65, 49.26, 40.08, 39.64, 39.45, 39.26, 28.08, 26.40, 22.51, 22.38, 22.25, 22.10, 21.93. HRMS (ESI-TOF) calcd. for C90H146N6O66 [M-2H]^2": 1 183.4126, found: 1 183.4122.

Biological assays

[0324] Mice immunity: The immunogenicity of the synthetic glycan conjugates were tested by mice serum assay. In the experimental group, female 6- to 8-week-old BALB/c mice (n = 5) were intramuscular administered conjugates containing 2 μg oligosaccharide in 100 μΐ_^ PBS buffer at two-week intervals. In addition, all antigens were formulated with 2 μg C34 or alum adjuvant. Blank PBS buffer was injected into mice as the control group. After seven days of the third boost, blood samples of each mice were collected for serological immune analysis by glycan microarray.

[0325] Glycan microarray for immunogenicity comparison. N. meningitidis W135 capsular disaccharide to decasaccharide compounds 13, 16, 19, 22, 25, and 70 other amine-contained oligosaccharides were printed on the NHS-coated glass slide. Detail of the printing procedure was described in supporting infomation. The microarray was designed 16 grids in one slide, and 20 columns x 8 rows in one grid. Printed slides were allowed reacting in an atmosphere of 80% humidity for an hour followed by desiccation overnight. Before serum antibody binding, the glycan microarrays were blocked with Superblock blocking buffer (Pierce) at 4 °C for one hour, followed by washing with PBST (PBS + 0.05% tween 20) buffer twice.

[0326] To investigate the immunogenicity of oligosaccharides having different lengthes, all collected sera were diluted 200 fold in PBST buffer containing 3% BSA. The diluents were incubated with the microarray at 4 °C for one hour to allow the induced antibodies binding to the oligosaccharide. Excess serum antibodies were washed out and the microarrays were incubated with goat anti-mouse IgG antibody labeled with fluorescence as the 2nd antibody at 4 °C for 1 hour. The slides were washed thoroughly and scanned at 635 nm wavelength with a microarray fluorescence chip reader.

[0327] In the group employed C34 as an adjuvant, mice immunized with DT-2 elicited antibody against N. meningitidis serogroup W135 capsular disaccharide compound 13, but cannot cross react with tetrasaccharide or longer oligosaccharide. The antibody also recognized other similar disaccharide compounds on the chip, including Neu5Gc-ct-(l→6)-Gal-a-(2→ and Neu5Ac-a- (1→6)-Gal-P"(2— >. In contrast, the DT-4 induced antibodies can bind to N. meningitidis serogroup W135 capsular oligosaccharide compounds 16, 19, 22, and 25, but cannot recognize disaccharide compound 13 or other oligosaccharides on the slide (Figure 6). Antibodies elicited from DT-6, DT-8, and DT-10 also performed the same pattern as DT-4 induced antibodies on the microarray. Therefore, it indicated that antibodies induced by DT-4, DT-6, DT-8, and DT-10 were very similar but different from antibodies induced by DT-2. Moreover, based on the fluorescence intensity analysis of glycan array, the common phenomenon is that DT-4, DT-6, DT-8 and DT-10 induced antibodies bound to longer oligosaccharide with higher affinity and DT-8 induced most abundent antibody titers (Figure 2).

[0328] In the group that employed alum as adjuvant, the antibodies induced by DT-4, DT-6, DT-8, and DT-10 also differed from DT-2 (Figure 7), and the patterns were very similar with the vaccines that used C34 as an adjuvant. However, the induced antibodies titer of alum adjuvant was lower than C34.

[0329] When carbohydrate itself is used as an antigen, the immune system processes thymus- independent (TI) response, and IgM is the predominant antibody. On the other hand, the carbohydrate-protein conjugates elicit thymus-dependent (TD) response (a) K. E. Stein, D. A. Zopf, B. M. Johnson, C. B. Miller, W. E. Paul, J Immunol 1982, 128, 1350-1354; b) . E. Stein, J Infect Dis 1992, 165 Suppl 1 , S49-52), and the ratio of IgG and IgM changes. Here, DT-8/C34 induced serum antibodies were used for further analysis. DT-8 induced IgG and IgM antibody titers were determined by microarray. Then, the antibody titer was defined to be the most dilution fold when the fluorescence s/N ratio higher than 3. The results showed the anti-DT-8 IgG antibody titer was greater than 5* 106. However, the titer of IgM antibody was only 200 (Table 2 and Table 3), indicating the use of TD-antigen resulted in switching antibody isotype.

Table 2. Mice serum IgG antibody titer.

Glycan microarray was used to detect the production of IgG in the serum. The fluorescence signal to noise ratio (S/N) higher than 3 was detectable. The IgG antibody titer was higher than 500000.

Table 3. Mice serum IgM antibody titer.

Glycan microarray was used to detect the production of IgM in the serum. The fluorescence signal to noise ratio (S/N) higher than 3 was detectable. The IgM antibody titer was only 200. Together with the IgG antibody titer result, the oligosaccharide-protein conjugate is

predominantly elicited IgG antibody.

[0330] The IgG subclasses distribution was tested by incubating the oligosaccharide-coated microarray with 2^nd anti-mouse IgGl, IgG2a, IgG2b, IgG2c, and IgG3 antibodies after the serum antibody binding. The anti-IgG antibody in serum contained IgGl, IgG2b, IgG2c, and IgG3 but no significant IgG2a (Figure 8). For the TD-antigen, the IgGl subclass was highest in the serum. Particularly, IgG3, a typical anti-carbohydrate antibody (R. M. Perlmutter, D. Hansburg, D. E. Briles, R. A. Nicolotti, J. M. Davie, Journal of Immunology 1978, 121 , 566-572), displayed a high level in the serum.

[0331] Serum bactericidal assay (SBA): The -80 °C stock culture of N. meningitidis serogroup W135 strain was streaked and incubated overnight at 37 °C with 5% CO₂. The strain was sub- cultured by spreading ~20 cfu (colony forming unit) over an entire BHI-HS (brain heart infusion-1% horse serum) agar plate and incubated for 4 hours at 37 °C with 5% CO₂. After 4 hours, the bacteria were suspended in normal saline and adjusted to a concentration of 10⁵ cfu/ml. The antisera were two-fold serial diluted (1/2 to 1/32) with normal saline. The 10 μΐ of diluted antisera and 20 μΐ of bacteria suspension (2000 cfu) were incubated at 37 °C with 5% CO₂ for 15 minutes. After incubation, 25 μΐ of new born rabbit complement (Pel-Freez, USA) and 25 μΐ of normal saline were added and incubated for 1 hour at 37 °C with 5% CO₂. Then 2 μΐ of the reaction mixture was placed on the BHI-HS plate. After overnight culture, the numbers of surviving bacteria were counted. SBA titers were defined as the reciprocal of the serum dilution that resulted in > 50% killing of the bacteria that achieved with the bacteria- complement-buffer controls.

[0332] The results showed that the bactericidal ability was roughly correlated with the antibody level in microarray. The SBA titer in mice immunized with DT-4 and DT-8 were 1/8 and 1/16 (Table 4). Note that the SBA titer of DT-10 is only 1/4.

Table 4. The serum bactericidal titers

W

:OT-€C3

PT 8< 3 1/16

PT-K C34

Bactericidal tilter from sera of mice immunized with different length oligosaccahride conjugating with DT. Serum from mice immunized with DT-2 displayed no bactericidal activity. DT-4 or longer length oligsaccahride conjugates induced antibody with bactericidal ability.

[0333] All publications and patent applications cited in this specification are herein incorporated by reference as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

[0334] Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the appended claims.

Claims

What is claimed is:

1. A compound of Formula (F- 1 )

(F-l)

or a salt thereof,

wherein

1 2 3 4 5 6 1

each instance of R , R , R , R , R , R , and R is independently hydrogen, optionally substituted Ci_6 alkyl, or an oxygen protecting group; or optionally R¹ and R² are taken with the intervening atoms to form a heterocyclic ring; or optionally R² and R³ are taken with the intervening atoms to form a heterocyclic ring; or optionally R⁵ and R⁶ are taken with the intervening atoms to form a heterocyclic ring; or R^N1 and R⁰¹ are taken together with the intervening atoms to form a heterocyclic ring;

each instance of R^N1 and R^N2 is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group;

each instance of R⁰¹ is independently hydrogen, optionally substituted Ci_₆ alkyl, or an oxygen protecting group;

B is -N₃ or (R^BN)₂;

each instance of R^BN is independently hydrogen, optionally substituted Ci- alkyl, optionally substituted acyl, or a nitrogen protecting group;

n is an integer of 1 to 100, inclusive; and

p is an integer of 1 to 10, inclusive.

2. The compound of claim 1 , wherein the compound is of Formula (F-2)

3. A glycan conjugate or a pharmaceutically acceptable salt thereof, comprising a carrier and a glycan moiety of Formula (I)

(I)

wherein:

the glycan moiety is covalently linked to the carrier through a linker -L-;

each instance of R 1, R2, R 3, R 4, R 5, R 6, and R 7 is independently hydrogen, optionally substituted Ci_6 alkyl, or an oxygen protecting group; or optionally R¹ and R² are taken with the intervening atoms to form a heterocyclic ring; or optionally R² and R³ are taken with the intervening atoms to form a heterocyclic ring; or optionally R⁵ and R⁶ are taken with the intervening atoms to form a heterocyclic ring; or optionally R⁶ and R⁷ are taken with the intervening atoms to form a heterocyclic ring; or R^N1 and R⁰¹ are taken together with the intervening atoms to form a heterocyclic ring;

each instance of R^N1, R^N2, and R^BN is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group;

each instance of L is independently a bond, -C(=0)-, -C(=0)NR^La- -C(=0)S- - C(=0)0- -C(=S)NR^L

-C≡C-, -C(R^Lb)₂0-, - C(R^Lb)₂NR^L -, -C(R^Lb)₂S- -S(=0)₂0-, -S(=0)₂NR -, or an optionally substituted Ci-30 hydrocarbon chain, optionally wherein one or more carbon units of the hydrocarbon chain is replaced with -0-, -S-, -NR^La- -C(=0)-, NR^LaC(=0)-, -NR^LaC(=0)0-, -C(=0)NR^La-, - OC(=0)NR^La-, -SC(=0)-, -C(=0)S-, -OC(=0)-, -C(=0)0-, -NR^LaC(=S)-, -C(=S)NR^La- trans-CR^Lh=CR^Lh- c«-CR^Lb=CR^Lb- -C≡C-, -S(=0)₂0-, -OS(=0)₂- -S(=0)₂NR^La- or - NR^LaS(=0)₂-, wherein each instance of R^La is hydrogen, optionally substituted C MS alkyl, or a nitrogen protecting group, or R^La is joined with the adjacent carbon atom to form an optionally substituted heterocyclic ring, and wherein each occurrence of R^Lb is independently selected from the group consisting of hydrogen, halogen, optionally substituted CMS alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl, or R^Lb is joined with the adjacent carbon or nitrogen or oxygen atom to form an optionally substituted carbocyclic or heterocyclic ring, or two R^Lb groups are joined to form an optionally substituted carbocyclic or optionally substituted heterocyclic ring;

p is an integer of 1 to 10, inclusive; and

n is an integer of 1 to 100, inclusive.

(I-a)

wherein

each instance of L¹ is independently a bond, -0-, -S-, -NR^Lla-, -C(=0)-, -

OC(=0)-, -C(=0)0- -NR^LlaC(=S)-, -C(=S)NR^Lla- irans-CR^Llb=CR^Llb- cw-CR^Llb=CR^Llb- -C≡C-, -OC(R^Llb)₂- -C(R^Llb)₂0-, -NR^LlaC(R^Llb)₂- -C(R^Llb)₂NR^Lla- -SC(R^Llb)₂- - C(R^Llb)₂S-, -S(=0)₂0-, -OS(=0)₂-, -S(=0)₂NR^Lla-, -NR^LlaS(=0)₂-, or an optionally substituted Ci_₂o hydrocarbon chain, optionally wherein one or more carbon units of the hydrocarbon chain is replaced with -0-, -S-, -NR^Lla- -C(=0)-, NR^LlaC(=0)-, - NR^LlaC(=0)0- -C(=0)NR^Lla- -OC(=0)NR^Lla-, -SC(=0)-, -C(=0)S-, -OC(=0)-, - C(=0)0- -NR^LlaC(=S)-, -C(=S)NR^Lla-, trans-CR^Llb=CR^Llb-, czs-CR^Llb=CR^Llb- -C≡C-, - S(=0)₂0-, -OS(=0)₂-, -S(=0)₂NR^Lla-, or -NR^LlaS(=0)₂-, wherein each instance of R^Lla is hydrogen, optionally substituted Ci_6 alkyl, or a nitrogen protecting group, or R^Lla is joined with the adjacent carbon atom to form an optionally substituted heterocyclic ring, and wherein each occurrence of R^Llb is independently selected from the group consisting of hydrogen, halogen, optionally substituted Ο_1-10 alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl, or R^Llb is joined with the adjacent carbon or nitrogen or oxygen atom to form an optionally substituted carbocyclic or heterocyclic ring, or two R^Llb groups are joined to form an optionally substituted carbocyclic or optionally substituted heterocyclic ring;

each instance of L² is independently a moiety derived from a crosslinking reagent capable of crosslinking the carrier and L'-H;

each instance of L^3C is independently a crosslinking reagent capable of crosslinking the carrier and L¹-!!;

each instance of R^CN is independently hydrogen, optionally substituted Ci_6 alkyl, optionally substituted acyl, or a nitrogen protecting group;

w is a integer of 1 to 100, inclusive; and

y is 0 or an integer of 1 to 100, inclusive.

5. The glycan conjugate of claim 4, wherein w is an integer of 1 to 10, inclusive.

6. The glycan conjugate of any one of claims 4-5, wherein y is 0 or an integer of 1 to 10, inclusive.

7. The glycan conjugate of any one of claims 4-6, wherein p is 5.

8. The glycan conjugate of any one of claims 4-7, wherein R¹, R², R⁴, R⁵, R⁶, and R⁷ are hydrogen.

9. The glycan conjugate of any one of claims 4-8, wherein R^N1 is acetyl.

10. The glycan conjugate of any one of claims 4-9, wherein R^N2 is hydrogen.

11. The glycan conjugate of any one of claims 4-10, wherein R is hydrogen.

12. The glycan conjugate of any one of claims 4-11, wherein R^CN is hydrogen.

13. The glycan conjugate of any one of claims 3-12, wherein the carrier is a protein, a lipid, a lipolized protein, a virus, a peptide comprising a T cell epitope, or a dendrimer of glycopeptides.

14. The glycan conjugate of claim 13, wherein the carrier is a toxin protein selected from the group consisting of diphtheria toxin cross-reacting material 197 (DT-CRM197), diphtheria toxoid, tetanus toxoid, and outer-membrane protein (OMP).

15. The glycan conjugate of claim 14, wherein the toxin protein is DT-CRM197.

17. A glycan conjugate mixture comprising at least two of the glycan conjugates of any one of claims 3-16.

18. The glycan conjugate mixture of claim 17, wherein the average value of w in the mixture is from about 1.0 to about 100.0.

19. The glycan conjugate mixture of claim 18, wherein the average value of w in the mixture is from about 1.0 to 10.0.

20. The glycan conjugate mixture of claim 19, wherein the average value of w is about 5.7, 4.9, 2.9, 2.8, or 3.1.

21. An immunogenic composition, comprising

(i) a glycan conjugate of any one of claims 3-16 or a glycan conjugate mixture of any one of claims 17-20; and

(ii) a pharmaceutically acceptable excipient.

22. The immunogenic composition of claim 21 , further comprising an adjuvant.

23. The immunogenic composition of claim 36, wherein the adjuvant is C34, 7DW8-5, C17, C23, C30, a-galactoceramide, Aluminum salt, Squalene, MF59, or QS-21.

24. A method of treating or reducing the risk for an infectious disease in a subject, the method comprising administering to the subject an effective amount of a glycan conjugate according to any one of claims 3-16, a glycan conjugate mixture of any one of claims 17-20, or an immunogenic composition according to any one of claims 21-23.

25. The method of claim 24, wherein the infectious disease is a bacterial infection.

26. The method of claim 25, wherein the bacterial infection is an infection with a Gram- positive bacterium.

27. The method of claim 26, wherein the Gram-positive bacterium is selected from the group consisting of Staphylococcus, Streptococcus, Micrococcus, Peptococcus, Peptostreptococcus, Enterococcus, Bacillus, Clostridium, Lactobacillus, Listeria, Erysipelothrix, Propionibacterium, Eubacterium, and Corynebacterium.

28. The method of claim 25, wherein the bacterial infection is an infection with a Gram- negative bacterium.

29. The method of claim 28, wherein the Gram-negative bacterium is selected from the group consisting of Escherchia coli, Caulobacter crescentus, Pseudomonas aeruginosa, Agrobacterium tumefaciens, Branhamella catarrhalis, Citrobacter diversus, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, Salmonella typhimurium, Neisseria meningitidis, Serratia marcescens, Shigella sonnei, Neisseria gonorrhoeae, Acinetobacter baumannii, Salmonella enteriditis, Fusobacterium nucleatum, Veillonella parvula, Bacteroides forsythus, Actino bacillus actinomycetemcomitans , Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Helicobacter pylori, Francisella tularensis, Yersinia pestis,

Morganella morganii, Edwardsiella tarda, Acinetobacter baumannii and Haemophilus influenzae.

30. The method of claim 28, wherein the Gram-negative bacterium is Neisseria meningitides .

31. The method of claim 30, wherein the Gram-negative bacterium is Neisseria meningitides serogroup W135.

32. A method for inhibiting bacterial cell growth comprising contacting the bacterial cell with a glycan conjugate according to any one of claims 3-16, a glycan conjugate mixture of any one of claims 17-20, or an immunogenic composition according to any one of claims 21-23.

33. The method of claim 32, wherein the contacting step is performed in in vitro.

34. The method of claim 33, wherein the contacting step is performed by administering the glycan conjugate according to any one of claims 3-16, the glycan conjugate mixture of any one of claims 17-20, or the immunogenic composition according to any one of claims 21-23, to a subject in need thereof.

35. A kit comprising a glycan conjugate according to any one of claims 3-16, a glycan conjugate mixture of any one of claims 17-20, or an immunogenic composition according to any one of claims 21-23, and instructions for use thereof.

36. A method of preparing a glycan conjugate of claim 4, comprising coupling a compound of Formula (C-l)

(C-l)

or a salt thereof,

with a compound of the Formula (C-2)

(C-2)

wherein

L^2C is a crosslinking reagent capable of crosslinking an amino group and L'-H.

37. The method of claim 30, wherein L is a crosslinking reagent capable of crosslinking an amine group and -SH.

38. The method of cl lae:

or a salt thereof,

wherein

each instance of R^P1 and R^P2are each independently hydrogen, halogen, or optionally substituted Ci_6 alkyl;

each instance of R^2Ca is a leaving group selected from selected from -Br, -CI, -I, - OS(=0)₂R^2CO, or -OS(=0)R^2CO, wherein R^2C0 is selected from the group consisting of optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl; and

each of t and i is independently an integer of 1 to 8, inclusive.

39. The method of any one of claim 36-38, wherein the molar ratio of the compound of Formula (C-1) to the compound of Formula (C-2) is from about 1 to about 100.

40. The method of any one of claims 36-39, wherein the coupling is carried out in the presence of phosphate buffered saline (PBS).

41. The method of any one of claims 36-40, further comprising glycosylating a compound of Formula (C-3)

(C-3)

ith a compound of Formula (C-4)

(C-5) wherein

v is an integer of 1 to 99, inclusive;

u is 0, 1 , 2, 3, 4, or 5;

each occurrence of R^E is independently halogen, -CN, -NO₂, -N3, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted phenyl, optionally substituted heterocyclyl, optionally substituted heteroaryl, -OR^CE, -N(R^CE)₂, -SR^CE, -C(=0)R^CE, -C(=0)OR^CE or -C(=0)N(R^CE)₂, wherein each R^CE is independently selected from the group consisting of hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted heterocyclyl, optionally substituted aryl, and optionally substituted heteroaryl; or an oxygen protecting group when attached to an oxygen, or a nitrogen protecting group when attached to nitrogen, or a sulfur protecting group when attached to sulfur; and

R^N3 is independently hydrogen, optionally substituted alkyl, or a nitrogen protecting group.

42. The method of claim 41 , further comprising reacting the compound of Formula (C-5) in the presence of a base to give a compound of Formula (C-6)

(C-6)

43. The method of claim 42, wherein the base is NaOCH3.

44. The method of claim 42, further comprising reacting the compound of Formula (C-6) with a reducing agent to give a compound of Formula (C-7)

(C-7)

45. The method of any one of claim 44, further comprising

(a) activating a compound of Formula (C-7) to give a compound of Formula (C-l); and

(b) activating a carrier to give a compound of Formula (C-2).