WO2014196033A1

WO2014196033A1 - Method for producing tobacco material

Info

Publication number: WO2014196033A1
Application number: PCT/JP2013/065589
Authority: WO
Inventors: 貴純香島
Original assignee: 日本たばこ産業株式会社
Priority date: 2013-06-05
Filing date: 2013-06-05
Publication date: 2014-12-11

Abstract

Provided is a method for producing a tobacco material that is reduced in the TSNA content and the ammonia content. A method for producing a tobacco material, which comprises an extraction step wherein tobacco leaves are subjected to extraction using an aqueous solution that contains a salt dissolved and satisfies at least one of the conditions (1) and (2) described below, and wherein the extraction residue obtained in the extraction step is used as a tobacco material. (1) The salt is a salt of a polybasic acid; the concentration of the cation species of the salt when the aqueous solution is prepared is 0.16 mol/g or more; and the pH of the aqueous solution when the tobacco leaves are subjected to extraction is 7.98 or more. (2) The concentration of the cation species of the salt when the aqueous solution is prepared is 0.23 mol/g or more; and the pH of the aqueous solution when the tobacco leaves are subjected to extraction is 8.60 or more.

Description

Tobacco raw material manufacturing method

The present invention relates to a method for producing a tobacco raw material. More specifically, the present invention relates to a method for producing a tobacco raw material that can be used for an oral tobacco product.

Conventional attempts have been made to selectively remove specific components from tobacco leaves. For example, in Patent Document 1, in order to selectively remove nitrosamine (TSNA) contained in tobacco leaves, an operation of extracting tobacco leaves using water as a solvent is first performed, and an aqueous extract obtained by the operation is extracted. On the other hand, a method is described that comprises contacting TSNA with an adsorbent that selectively adsorbs TSNA. In the method described in Patent Document 1, it is described that filtered water, distilled water, deionized water, common plug water, or an aqueous solution is used as water used to obtain an aqueous extract.

Patent Document 2 describes a method for modifying the characteristics of tobacco materials. After obtaining a tobacco material extract extracted with an aqueous solvent, the pH of the solvent of the extract is set to 9 or more. It is described that a tobacco material extract (aqueous denicotine tobacco material extract) with reduced nicotine can be obtained through a treatment including a step of bringing the product into contact with another solvent.

Patent Document 3 describes a method for treating tobacco, which includes a step of extracting tobacco with an aqueous or organic solvent and a step of filtering the extracted product, and these steps are performed on a horizontal belt filter.

Special table 2010-516272 Japanese Patent Laid-Open No. 5-7485 Special table 2011-522558 gazette

In the method described in Patent Document 1, it is described that TSNA is removed using an adsorbent, so that alkaloids are not reduced so much while TSNA is removed.
In the method described in Patent Document 2, it is described that the pH of water, which is a solvent for obtaining an aqueous denicotine tobacco material extract, may be adjusted, but the effect obtained thereby is described. Absent.
The method described in Patent Document 3 is premised on performing an extraction operation and filtration on a horizontal belt.
In the methods described in

Patent Documents

1 and 2, a multi-stage process is disclosed as a process for treating tobacco leaves. In Patent Document 1, it is assumed that a special adsorbent is used. In the method described in Patent Document 3, it is assumed that a special apparatus is used.
For these reasons, there is a demand for a technique that does not change the content of nicotine in tobacco leaves while selectively removing components such as TSNA and ammonia from tobacco leaves by a simple method.

Therefore, in the present invention, a tobacco raw material in which the content of TSNA and ammonia in tobacco leaves is reduced while the change in the content of nicotine in tobacco leaves is suppressed is easily produced. Provide a method.

As a result of intensive studies by the inventor, the extraction residue obtained by extracting tobacco leaves by a specific extraction process is reduced in terms of TSNA and ammonia content, while the change in nicotine content is suppressed. The present invention was reached.

That is, the present invention is as follows.
[1] Tobacco including an extraction step of extracting tobacco leaves with an aqueous solution satisfying at least one of the following requirements (1) and (2) in which a salt is dissolved, and using the extraction residue obtained in the extraction step as a tobacco raw material Raw material manufacturing method.
(1) The salt is a polybasic acid salt, the concentration of the cation species of the salt at the time of preparation of the aqueous solution is 0.16 mol / g or more, and the pH of the aqueous solution at the time of extraction of the tobacco leaves is 7. 98 or more.
(2) The concentration of the cation species of the salt at the time of preparation of the aqueous solution is 0.23 mol / g or more, and the pH of the aqueous solution at the time of extraction of the tobacco leaf is 8.60 or more.
[2] The aqueous solution is an aqueous solution satisfying the requirement (1), and the salt of the polybasic acid is sodium carbonate, potassium carbonate, tripotassium phosphate, tripotassium citrate, sodium sulfate, ammonium sulfate, dipotassium hydrogen phosphate The production method according to [1], which is at least one selected from disodium hydrogen phosphate, sodium hydrogen carbonate, and trisodium phosphate.
[3] The production method according to [2], wherein the concentration of the cationic species of the salt during preparation of the aqueous solution is 0.28 mol / g or more, and the pH during extraction of the tobacco leaf is 7.98 or more.
[4] The production method according to [2], wherein the concentration of the cationic species of the salt at the time of preparing the aqueous solution is 0.49 mol / g or more, and the pH at the time of extraction of the tobacco leaves is 7.98 or more.
[5] The production method according to [2], wherein the concentration of the cationic species of the salt at the time of preparing the aqueous solution is 0.57 mol / g or more, and the pH at the time of extraction of the tobacco leaves is 7.98 or more. 6] The aqueous solution is an aqueous solution satisfying the requirement (2), the salt to be dissolved is a salt of a monobasic acid or a mixture of a polybasic acid and a salt of a monobasic acid, and the cation species of the salt at the time of preparing the aqueous solution The production method according to [1], in which the concentration of is at least 0.23 mol / g and the pH at the time of extraction of the tobacco leaves is 8.60 or more.
[7] The production method according to [6], wherein the concentration of the cationic species of the salt at the time of preparing the aqueous solution is 0.34 mol / g or more, and the pH at the time of extraction of the tobacco leaves is 8.60 or more.
[8] The production method according to [6], wherein the concentration of the cationic species of the salt during preparation of the aqueous solution is 0.38 mol / g or more, and the pH during extraction of the tobacco leaves is 8.60 or more.
[9] The production method according to [6], wherein the concentration of the cation species of the salt during preparation of the aqueous solution is 0.42 mol / g or more, and the pH during extraction of the tobacco leaf is 10.12 or more.
[10] The production method according to [6], wherein the concentration of the cation species of the salt during preparation of the aqueous solution is 0.57 mol / g or more, and the pH during extraction of the tobacco leaf is 10.12 or more.
[11] The production method according to any one of [6] to [10], wherein the salt to be dissolved is a salt of a monobasic acid, and the salt of the monobasic acid is sodium chloride or potassium chloride.
[12] The salt to be dissolved is a mixture of a polybasic acid salt and a monobasic acid salt, the monobasic acid salt is sodium chloride or potassium chloride, and the polybasic acid salt is sodium carbonate or potassium carbonate. [6] to [6]-[one or more selected from tripotassium phosphate, tripotassium citrate, sodium sulfate, ammonium sulfate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium hydrogen carbonate and trisodium phosphate. [10] The production method according to any one of [10].

According to the present invention, there is provided a method for producing a tobacco raw material by a simple method in which the content of TSNA and ammonia is reduced while the change in the content of nicotine is suppressed.

It is a figure which shows the relationship between the density | concentration of the cation seed | species in the aqueous solution for every salt, and the extraction suppression rate of nicotine. It is a figure which shows the relationship between the pH of aqueous solution at the time of tobacco leaf extraction, and the density | concentration of a cation seed | species for every extraction suppression rate. In FIG. 2, an example using only a salt of a polybasic acid is extracted, and the range of the concentration and pH of the cation species capable of obtaining the extraction suppression rate in a specific range are illustrated. FIG. 2 illustrates the concentration and pH range of a cation species that can obtain an extraction suppression rate in a specific range.

Hereinafter, the present invention will be described in detail with reference to embodiments, examples, etc., but the present invention is not limited to the following embodiments, examples, etc., and can be arbitrarily set within the scope of the present invention. Can be changed and implemented.

The present invention includes an extraction step of extracting tobacco leaves with an aqueous solution satisfying at least one of the following requirements (1) and (2) in which a salt is dissolved.
(1) The salt is a polybasic acid salt, the concentration of the cation species of the salt at the time of preparation of the aqueous solution is 0.16 mol / g or more, and the pH of the aqueous solution at the time of extraction of the tobacco leaves is 7. 98 or more.
(2) The concentration of the cation species of the salt at the time of preparation of the aqueous solution is 0.23 mol / g or more, and the pH of the aqueous solution at the time of extraction of the tobacco leaf is 8.60 or more.

In the present invention, the concentration of the cation species in the aqueous solution is the concentration at which the aqueous solution used for the extraction treatment is prepared. In the present invention, the pH at the time of extraction of the aqueous solution is a value obtained when the pH of the aqueous solution after the extraction treatment is measured, as will be described later.

In the requirement (1) above, the concentration of the cationic species of the salt when preparing the aqueous solution is 0.16 mol / g or more, and the pH at the time of extraction of tobacco leaves using this is 7.98 or more. Thus, the content of TSNA and ammonia in the tobacco leaf can be effectively reduced, while maintaining the nicotine content. At this time, the amount of nicotine extracted and reduced from the tobacco leaf (hereinafter also referred to as nicotine extraction rate) can be suppressed by at least 10% (FIG. 3 (1)).
The concentration of the cationic species of the salt when preparing the aqueous solution is 0.28 mol / g or more, and the pH at the time of extraction of tobacco leaves using the salt is 7.98 or more. While reducing the content of TSNA and ammonia, the extraction rate of nicotine can be suppressed by at least 30% (FIG. 3 (2)).
Further, when the concentration of the cationic species of the salt when preparing the aqueous solution is 0.49 mol / g or more, and the pH at the time of extraction of the tobacco leaf using the salt is 7.98 or more, the tobacco leaf While reducing the content of TSNA and ammonia therein, the extraction rate of nicotine can be suppressed by at least 40% (FIG. 3 (3)).
Furthermore, when the concentration of the cationic species of the salt when preparing the aqueous solution is 0.57 mol / g or more, and the pH at the time of extraction of the tobacco leaf using the salt is 7.98 or more, the tobacco leaf While reducing the content of TSNA and ammonia therein, the extraction rate of nicotine can be suppressed by at least 70% (FIG. 3 (4)).

In the present invention, at least the X% extraction rate is suppressed, as will be shown below, the extraction of nicotine originally contained in tobacco leaves is suppressed by at least X% compared to the case of extraction with pure water. It means that
For nicotine contained in tobacco leaves, the total amount of nicotine in tobacco leaves was determined by combining the amount in the extract and the amount in the residue. The extraction rate and extraction inhibition rate of nicotine were defined as follows.
Extraction rate of nicotine (%) = the amount of nicotine in the extract of each sample / the amount of total nicotine × 100
Nicotine extraction inhibition rate (%) = (1−nicotine extraction rate of each sample / nicotine extraction rate with pure water) × 100

The salt of the polybasic acid used to satisfy the requirement (1) is not particularly limited as long as it is an acid salt having a basicity of 2 or more, but sodium carbonate, potassium carbonate, tripotassium phosphate, citric acid, and the like. One or more kinds selected from tripotassium acid, sodium sulfate, ammonium sulfate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium hydrogen carbonate and trisodium phosphate can be mentioned. Even when any of these salts is used, the effect of the present invention can be obtained if the requirement (1) is satisfied.
Among these, it is preferable to use a metal salt.

In the requirement (2) above, the concentration of the cationic species of the salt when preparing the aqueous solution is 0.23 mol / g or more, and the pH at the time of extraction of tobacco leaves using this is 8.60 or more. Thus, the content of TSNA and ammonia in the tobacco leaf can be effectively reduced, while maintaining the nicotine content. At this time, the amount of nicotine extracted and reduced from the tobacco leaf (hereinafter also referred to as nicotine extraction rate) can be suppressed by at least 10% (FIG. 4 (5)).
The concentration of the cationic species of the salt when preparing the aqueous solution is 0.34 mol / g or more, and the pH at the time of extraction of tobacco leaves using the salt is 8.60 or more. While reducing the content of TSNA and ammonia, the extraction rate of nicotine can be suppressed by at least 20% (FIG. 4 (6)).
The concentration of the cationic species of the salt when preparing the aqueous solution is 0.38 mol / g or more, and the pH at the time of extraction of tobacco leaves using the salt is 8.60 or more. While reducing the content of TSNA and ammonia, the extraction rate of nicotine can be suppressed by at least 25% (FIG. 4 (7)).
When the concentration of the cationic species of the salt when preparing the aqueous solution is 0.42 mol / g or more, and the pH at the time of extraction of tobacco leaves using this is 10.12 or more, While reducing the content of TSNA and ammonia, the extraction rate of nicotine can be suppressed by at least 30% (FIG. 4 (8)).
When the concentration of the cationic species of the salt in preparing the aqueous solution is 0.57 mol / g or more, and the pH at the time of extraction of tobacco leaves using this is 10.12 or more, While reducing the content of TSNA and ammonia, the extraction rate of nicotine can be suppressed by at least 70% (FIG. 4 (9)).

Examples of the salt used to satisfy the requirement (2) above include an embodiment using only a monobasic acid salt and an embodiment combining a monobasic acid salt and a polybasic acid salt.
Examples of monobasic acid salts include sodium chloride and potassium chloride. Polybasic acid salts include sodium carbonate, potassium carbonate, tripotassium phosphate, tripotassium citrate, sodium sulfate, ammonium sulfate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium bicarbonate and trisodium phosphate. One or more selected may be mentioned.
Examples of the salt of the polybasic acid when used in combination with the salt of the monobasic acid include combinations with the salts of the polybasic acid mentioned above.
When a monobasic acid salt is used without being combined with a polybasic acid salt, the aqueous solution is dissolved in water such as sodium hydroxide or potassium hydroxide, which will be described later, in order to adjust the pH. The pH can be adjusted to basic by dissolving a basic substance in an aqueous solution.
About the upper limit of pH at the time of satisfy | filling the requirements of said (1) and (2), it can be set to 14 in any case.

The upper limit of the concentration of the cationic species of the salt when satisfying the above requirements (1) and (2) is determined according to the solubility of various salts, and the upper limit is the concentration until the salt to be dissolved is dissolved.
The concentration of the cation species of the salt is calculated based on the total amount of the cation species of the salt to be dissolved.
The upper limit concentration of the salt to be dissolved can be determined, for example, with reference to the solubility in water described in the following table.

The pH of the aqueous salt solution used in the production method of the present invention may be adjusted by adjusting the addition amount of the above-mentioned salt alone, and sodium hydroxide, potassium hydroxide or the like may be used to adjust the pH. It may be carried out by adjusting the amount of the substance that makes the aqueous solution basic when dissolved in pure water alone.
These addition amounts can be appropriately adjusted so that the pH of the aqueous solution falls within a predetermined range defined in the present invention.

In the production method of the present invention, a tobacco leaf is extracted using a specific aqueous solution. However, the weight ratio of the tobacco leaf to the aqueous solution during the extraction treatment is not particularly limited, and the effects of the present invention are achieved. It can adjust suitably in the range which is not impaired.
The weight ratio between the tobacco leaf and the aqueous solution can be set in the range of about 1: 3 to 1:50. The water content of tobacco leaves extracted with an aqueous solution is about 8% by weight.
The temperature at the time of performing the extraction step is not particularly limited and can be appropriately adjusted and set. One embodiment includes setting the temperature in the range of 0 to 40 ° C. As another embodiment, it can be performed at about room temperature (10 to 30 ° C.).

Tobacco species used as a tobacco raw material in the present invention is not particularly limited, and examples thereof include the genus Nicotiana, and examples include Nicotiana tabacum yellow and Burley species and Nicotiana rustica brasilia species. These may be used alone or in combination with different types.
About the leaf of these tobacco seeds, after harvesting, after carrying out a normal process, the aspect which is cut | judged to a suitable magnitude | size for performing extraction and can use for a later extraction process can be mentioned.
The tobacco raw material that has undergone the extraction step can be provided as a tobacco product through normal processing such as filtration and drying.

<Tobacco products>
When the tobacco raw material of the present invention is an oral tobacco product, for example, snus can be cited as an example. In this case, the tobacco raw material produced by the above-described production method is obtained by filling a packaging material using a raw material such as a nonwoven fabric by a known method. For example, the amount of tobacco material is adjusted and filled, and sealed by means such as heat sealing to obtain snus.
The packaging material can be used without any particular limitation, but a cellulose-based nonwoven fabric is preferably used.
For example, when the gum is used as the oral tobacco product, it is obtained by mixing the tobacco raw material obtained using the production method of the present invention with a known gum base and a known method. A chewing tobacco, a hooked tobacco, and a compressed tobacco can also be obtained using a known method except that the tobacco raw material obtained using the production method of the present invention is used. Moreover, an edible film can also be obtained using a known material or method except that the tobacco raw material obtained using the production method of the present invention is used.

The production method of the present invention makes it possible to supply a tobacco raw material whose content component is modified to a desired one very easily. Specifically, since water-soluble components other than nicotine can be removed, it is possible to create a tobacco raw material that leaves a flavor component (nicotine) while removing miscellaneous taste caused by them. Therefore, it is possible to supply high-value-added raw materials for the oral tobacco products described above.

The present invention will be described more specifically with reference to examples. However, the present invention is not limited to the description of the following examples unless it exceeds the gist.

For each level shown in Table 2 below, an aqueous salt solution was prepared so as to have the composition described in the table, and tobacco leaves were extracted using it. For each level, the extraction rate and the extraction suppression rate were calculated. The amount of nicotine in the extract was measured by the following method, and the extraction rate and the extraction inhibition rate shown in Table 2 were calculated by the method described above using the values. In addition, Table 2 shows the pH of each level after extraction measured by the following method.

<Measurement of pH>
The ground tobacco leaf (Burley: Rustica: medium bone = 40: 20: 40 blend) and an aqueous solution were placed in a screw tube at a solid-liquid ratio of 1.0 mg / 10 mL, and extraction was performed by shaking for 30 minutes. After extraction, the pH was measured under the following conditions to obtain the pH value at the time of extraction.

<PH measurement conditions>
Measuring equipment: LAQUA F-72 flat ISFET pH electrode (Horiba)
Equipment calibration: 3 using phthalic acid pH standard solution (pH 4.01), neutral phosphate pH standard solution (pH 6.86), borate pH standard solution (pH 9.18) (all Wako Pure Chemical Industries) Point calibration

<Measurement of nicotine content in the extract>
Then, it separated into the extract and residue by filtration. The amount of nicotine in the extract was quantified by GC / NPD after phase transition to n-Hexane. The procedure is shown below. After sampling 0.5 mL of the extract into a screw tube and adding 2.0 mL of pure water and 2.5 mL of 22% aqueous sodium hydroxide, 5.0 mL of n-Hexane containing Quinoline at a concentration of 0.10 mg / mL was added. Liquid-liquid distribution was performed by shaking for 10 minutes. Quinoline contained in n-Hexane is an internal standard substance for quantification of nicotine by GC / NPD. After shaking, the organic layer was transferred to a GC measurement vial, and nicotine was quantified under the following GC conditions.

<GC measurement conditions>
Measuring instrument: Agilent 7890A GC
Carrier gas: He, 1.3 mL / min flow
Injector: ALS Sampler, Injection volume 1.0 μL
Inlet: Split-Splitless Inlet, Split Mode (Split Ratio 10: 1), Temp. 250 ℃
Column: DB-35MS, 30m, 0.25mm id, 0.25mm ft
Oven temperature: 100 ° C. (0.5 min) → (10 ° C./min) → 200 ° C. (0 min)
Detector: NPD, Temp. 325 ° C, H ₂ flow 3.0 mL / min, Air flow 120 mL / min, N ₂ flow 20 mL / min
The quantitative value is calculated by the amount of nicotine contained in the dry matter weight excluding the moisture of tobacco leaves subjected to extraction. The moisture content of the tobacco material was measured under the following conditions.

<Moisture measurement conditions>
Measuring equipment: Heat-drying moisture meter MX-50 (A & D)
Setting sample weight: 1.0 g
Heating temperature: 80 ° C

The results obtained at each level are described below.
Level 2 is a strong basic but a low salt concentration. Level 3 is a comparative example in which the salt concentration is high but the pH is not basic. From these comparisons, it can be seen that the effects of the present invention are not manifested under strong basic conditions alone or under conditions of high salt concentrations alone.
On the other hand, levels 4 to 8 are levels obtained by testing with extraction water adjusted to different pH depending on the amount of NaOH added after securing a certain high salt concentration condition with NaCl. From this result, it was found that under the condition where a high salt concentration was ensured, significant nicotine extraction suppression was expressed at a certain pH (8-9).

Next, levels 9 to 12 and 13 to 16 are levels obtained by extraction with aqueous solutions prepared to have different salt concentrations depending on the amount of NaCl or KCl added under the condition that certain basicity is secured with NaOH or KOH. From this result, it was found that the nicotine extraction inhibition rate increased in a salt concentration-dependent manner under a condition where a certain basicity was ensured. Moreover, it can be said that there is no difference in sodium and potassium about the cationic species of the salt to be used.
Furthermore, levels 17 to 21 (Na ₂ CO ₃ ), 22 to 27 (K ₂ CO ₃ ), 28 to 32 (K ₃ PO ₄ ), and 59 (Na ₃ PO ₄ ) are strongly basic in a single aqueous solution. It is the result of having tested using what is shown. Each of these levels guarantees a certain basic condition, an increase in the inhibition rate of nicotine extraction depending on the salt concentration is observed, the degree of effect on the salt concentration is almost the same, and NaOH and NaCl The effect on salt concentration was higher than levels 9-12, and 13-16, which consisted of combinations or combinations of KOH and KCl.
This result suggests that there is a difference in the effect on the concentration depending on the type of salt used under conditions where a certain basicity is ensured.

Next, levels 33-35 (trisodium citrate), 36-38 (potassium acetate), 51-53 (K ₂ HPO ₄ ), 57 (NaHCO ₃ ), 58 (Na ₂ HPO ₄ ) are used as a single aqueous solution. Then, it is tested using what shows weak basicity. Levels 33 to 34 and 36 to 37 are not effective because the pH is not sufficiently basic, but at levels 35 and 38, which are the highest concentration levels, the pH is around 8 and is notable. The effect was expressed. Compared with the levels 35 and 38, trisodium citrate was more effective, although the salt concentration and pH were almost equal. Judging comprehensively from these results and the results of levels 17 to 32, it was found that the use of a salt of a polybasic acid is more effective.
FIG. 1 shows the relationship between the type, the salt concentration, and the extraction inhibition rate of the salt used in which the salt concentration-dependent extraction inhibition effect was observed. First, it can be seen that the salt of polybasic acid has a higher extraction suppressing effect on the salt concentration. On the other hand, at levels 54 to 56 in which Na ₂ CO _3, which is a polybasic acid salt, and NaCl, which is a monovalent salt, are mixed, the nicotine extraction inhibitory effect on the concentration takes a value between Na ₂ CO ₃ and NaCl. Therefore, it is considered that the additivity of the effect of each salt is established when different types of salts are mixed.
Further, it can be said that the Na ₂ SO _₄ (NH ₄₎ than the difference of the effect on the salt concentration of ₂ SO _4, in the case of using a salt of a polybasic acid and it is more preferable to use a metal salt.
Finally, FIG. 2 shows a plot of all examples plotted with extraction pH and concentration of cationic species in the aqueous solution used for extraction. FIG. 3 and FIG. 4 show the results of analyzing this result.

<Analysis of TSNA>
The analysis of TSNA performed before and after the tobacco leaf extraction step followed the following procedure.
An internal standard was added to the tobacco sample, and the mixture was extracted by shaking with a 0.1 M aqueous ammonium acetate solution. The tobacco extract was diluted 10-fold with a 0.1M aqueous ammonium acetate solution and then filtered (pore size: 0.2 μm) to obtain a sample solution. TSNA in the sample solution was measured with an ultrahigh performance liquid chromatograph mass spectrometer (UPLC / MS / MS). N'-Nitrosonornicotine (NNN), (R, S) -N-Nitrosoanatabine (NAT), (R, S) -N-Nitrosoanabasine (NAB), 4- (Methylnitrosamino) -1- (3-Pyridyl ) -1-butanone (NNK) TSNA4 component was measured, and the total value of the four components was defined as the total TSNA amount.
Internal standard substances (IS) include DL-N'-nitrosonornicotine-2,4,5,6-d4 (dNNN) and 4- (N-methyl-N-nitrosoamino) -1- (3-pyridyl-d4) -1-butanone (dNNK) was used.

<Analysis of ammonia>
The analysis of ammonia performed before and after the tobacco leaf extraction process followed the following procedure.
Using a continuous flow analyzer (autoanalyzer), the tobacco or sample water extract was colored in an alkaline buffer solution using salicylic acid, hypochlorite, and sodium nitroprusside as a catalyst, and then the absorbance (wavelength 660 nm) was measured. Measured and quantified as ammonia ion (NH ₄ ⁺ ).
Measurements were made for

levels

0, 1, 7, and 20 shown in Table 2. Table 3 shows the measurement results.

From the results of Table 3 above, it can be seen that the total content of TSNA and the content of ammonia are effectively reduced after the extraction process that satisfies the conditions of the present invention.

<Experimental example>
An experiment was conducted in which the extraction treatment was performed by changing the ratio of the weight of the tobacco leaf to the weight of the aqueous solution. The tobacco leaf used was a mixture of different types, and extraction was performed with 5 mL, 10 mL, and 20 mL aqueous solution (20 wt% sodium carbonate aqueous solution) per 1 g of tobacco leaf. The results are shown in Table 4.
As shown in Table 4, it was found that even if the weight of the aqueous solution was changed with respect to the weight of the tobacco leaf, there was no significant difference in the nicotine extraction inhibition rate.

By providing tobacco raw materials with reduced TSNA and ammonia contents, it is possible to provide tobacco products that take advantage of these characteristics.
In the production method of the present invention, it is not necessary to perform complicated operations such as fractionation and chemical reaction, and time and cost can be reduced.

Claims

Production of tobacco raw materials including an extraction step of extracting tobacco leaves with an aqueous solution satisfying at least one of the following requirements (1) and (2) in which salt is dissolved, and using the extraction residue obtained in the extraction step as a tobacco raw material Method.
(1) The salt is a salt of a polybasic acid, the concentration of the cationic species of the salt at the time of preparation of the aqueous solution is 0.16 mol / g or more, and the pH of the aqueous solution at the time of extraction of the tobacco leaves is 7. 98 or more.
(2) The concentration of the cation species of the salt at the time of preparation of the aqueous solution is 0.23 mol / g or more, and the pH of the aqueous solution at the time of extraction of the tobacco leaf is 8.60 or more.
The aqueous solution is an aqueous solution satisfying the requirement (1), and the salt of the polybasic acid is sodium carbonate, potassium carbonate, tripotassium phosphate, tripotassium citrate, sodium sulfate, ammonium sulfate, dipotassium hydrogen phosphate, phosphoric acid The manufacturing method of Claim 1 which is 1 or more types chosen from disodium hydrogen, sodium hydrogencarbonate, and trisodium phosphate.
The production method according to claim 2, wherein the concentration of the cation species of the salt during preparation of the aqueous solution is 0.28 mol / g or more, and the pH during extraction of the tobacco leaf is 7.98 or more.
The production method according to claim 2, wherein the concentration of the cationic species of the salt at the time of preparation of the aqueous solution is 0.49 mol / g or more, and the pH at the time of extraction of the tobacco leaf is 7.98 or more.
The production method according to claim 2, wherein the concentration of the cationic species of the salt during preparation of the aqueous solution is 0.57 mol / g or more, and the pH during extraction of the tobacco leaf is 7.98 or more.
The aqueous solution is an aqueous solution satisfying the requirement (2), the salt to be dissolved is a salt of a monobasic acid or a mixture of a polybasic acid and a salt of a monobasic acid, and the concentration of the cation species of the salt at the time of preparation of the aqueous solution The production method according to claim 1, wherein the pH is 0.23 mol / g or more and the pH at the time of extraction of the tobacco leaves is 8.60 or more.
The production method according to claim 6, wherein the concentration of the cationic species of the salt during preparation of the aqueous solution is 0.34 mol / g or more, and the pH during extraction of the tobacco leaves is 8.60 or more.
The production method according to claim 6, wherein the concentration of the cationic species of the salt during preparation of the aqueous solution is 0.38 mol / g or more, and the pH during extraction of the tobacco leaves is 8.60 or more.
The production method according to claim 6, wherein the concentration of the cationic species of the salt during preparation of the aqueous solution is 0.42 mol / g or more, and the pH during extraction of the tobacco leaves is 10.12 or more.
The production method according to claim 6, wherein the concentration of the cationic species of the salt during preparation of the aqueous solution is 0.57 mol / g or more, and the pH during extraction of the tobacco leaf is 10.12 or more.
The production method according to any one of claims 6 to 10, wherein the salt to be dissolved is a salt of a monobasic acid, and the salt of the monobasic acid is sodium chloride or potassium chloride.
The salt to be dissolved is a mixture of a polybasic acid salt and a monobasic acid salt, the monobasic acid salt is sodium chloride or potassium chloride, and the polybasic acid salt is sodium carbonate, potassium carbonate, phosphoric acid. The composition according to any one of claims 6 to 10, which is at least one selected from tripotassium, tripotassium citrate, sodium sulfate, ammonium sulfate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium hydrogen carbonate and trisodium phosphate. The manufacturing method according to one item.