WO2014170345A2 - Method for improved utilization of the production potential of transgenic plants - Google Patents

Abstract

The invention relates to a method for improving the utilization of the production potential of transgenic plants by treating the plant with an effective amount of at least one compound of the formula (I) as described herein.

Description

Method for improved utilization of the production potential of transgenic plants

[0001] The invention relates to a method for improving the utilization of the production potential of transgenic plants and for controlling pests such as insects and/or nematodes.

[0002] In recent years, there has been a marked increase in the proportion of transgenic plants in agriculture.

[0003] Transgenic plants are employed mainly to utilize the production potential of respective plant varieties in the most favourable manner, at the lowest possible input of production means. The aim of the genetic modification of the plants is in particular the generation of resistance in the plants to certain pests or harmful organisms or else herbicides and also to abiotic stress (for example drought, heat or elevated salt levels). It is also possible to modify a plant genetically to increase certain quality or product features, such as, for example, the content of selected vitamins or oils, or to improve certain fibre properties.

[0004] Herbicide resistance or tolerance can be achieved, for example, by incorporating genes into the useful plant for expressing enzymes to detoxify certain herbicides, so that a relatively unimpeded growth of these plants is possible even in the presence of these herbicides for controlling broad-leaved weeds and weed grasses. Examples which may be mentioned are cotton varieties or maize varieties which tolerate the herbicidally active compound glyphosate (Roundup®), (Roundup Ready®, Monsanto) or the herbicides glufosinate or oxynil.

[0005] There has also been the development of useful plants comprising two or more genetic modifications ("stacked transgenic plants" or multiply transgenic crops). Thus, for example, Monsanto has developed multiply transgenic maize varieties which are resistant to the European corn borer

(Ostrinia nubilalis) and the Western corn rootworm (Diabrotica virgifera). Also known are maize and cotton crops which are both resistant to the Western corn rootworm and the cotton bollworm and tolerant to the herbicide Roundup®.

[0006] It has now been found that the utilization of the production potential of transgenic useful plants can be improved even more by treating the plants with one or more compounds of the formula (I) defined below. Here, the term "treatment" includes all measures resulting in a contact between these active compounds and at least one plant part. "Plant parts" are to be understood as meaning all above-ground and below-ground parts and organs of plants, such as shoot, leaf, flower and root, by way of example leaves, needles, stalks, stems, flowers, fruit bodies, fruits and seed, and also roots, tubers and rhizomes. The plant parts also include harvested material and also vegetative and generative propagation material, for example cuttings, tubers, rhizomes, slips and seed. Summary of the invention

[0007] One aspect refers to a method for improving the utilization of the production potential of a transgenic plant and/or for controlling/combating/treating pests, characterized in that the plant is treated with an effective amount of at least one compound of the formula (I)

wherein

A represents individually halogen, cyano, nitro, hydroxyl, amino, Ci-Cs alkyl group, substituted Ci-Cs alkyl group having at least one substituent elected from the group consisting of halogen, hydroxy, cyano, nitro, amino, halo C1-C3 alkyl group, C1-C3 alkoxy group, halo C1-C3 alkoxy group, C1-C3 alkylthio group, halo C1-C3 alkylthio group, C1-C3 alkylsulfinyl group, halo C1-C3 alkylsulfinyl group, C1-C3 alkylsulfonyl group, halo C1-C3 alkylsulfonyl group and C1-C3 alkylthio, C1-C3 alkyl group; further, an arbitrary saturated carbon atom in said optionally substituted Ci-Cs alkyl group; n represents 0, 1 , 2, 3 or 4, preferably 0, 1 or 2;

Ri represents hydrogen, halogen, cyano Ci-Cs alkyl or Ci-Cs haloalkyl;

R2 represents hydrogen, halogen, cyano Ci-Cs alkyl or Ci-Cs haloalkyl;

R3 represents O or S;

R represents O or S;

Y represents individually hydrogen, halogen, cyano, nitro, Ci-Ce alkyl group, halo Ci-Ce alkyl group, C2-C6 alkenyl group, halo C2-C6 alkenyl group, C2-C6 alkynyl group, halo C2-C6 alkynyl group, C3-C6 cycloalkyl group, halo C3-C6 cycloalkyl group, Ci-Ce alkoxy group, halo Ci-Ce alkoxy group, Ci-Ce alkylthio group, halo Ci-Ce alkylthio group, Ci-Ce alkylsulfinyl group, halo Ci-Ce alkylsulfinyl group, Ci-Ce alkylsulfonyl group, or halo Ci-Ce alkylsulfonyl group;

m represents 0, 1, 2, 3, or 4;

X represents a Ci-Cs alkyl group or a substituted Ci-Cs alkyl group having at least one substituent selected from the group consisting of halogen, hydroxy, cyano, nitro, amino, halo C1-C3 alkyl group, C1-C3 alkoxy group, halo C1-C3 alkoxy group [0008] One preferred embodiment refers to the method described above, characterized in that the compound of the formula (I) is formula (1-1):

wherein

Hal represents F, CI, I or Br; and

X' represents Ci-Ce alkyl or substituted Ci-Ce alkyl having at least one substituent selected from the group consisting of halogen, hydroxy, cyano, nitro, amino, halo C1-C3 alkyl group, preferably a Ci-C6 cyanoalkyl;

A' represents C1-C3 alkyl, C1-C3 haloalkyl, halogen, preferably methyl, halomethyl, ethyl or haloethyl, more preferably methyl or ethyl; n represents 0, 1 , 2, 3 or 4, preferably 0, 1 or 2, more preferably 1.

[0009] One preferred embodiment refers to the method described above, characterized in that the compound of the formula (I) is selected from the group consisting of compound (1-2), (1-3), (1-4) or

[0010] One preferred embodiment refers to the method described above, characterized in that the compound of the formula (I) is compound (1-5).

[0011] Further preferred embodiments refer to the method described above, characterized in that the plant has at least one genetically modified structure or a tolerance according to Table A or Table B or Table C.

[0012] Further preferred embodiments refer to the method described above, characterized in that the transgenic plant contains at least one cry-gene or a cry-gene fragment coding for a Bt toxin.

[0013] One preferred embodiment refers to the method described above, characterized in that the transgenic plant is a vegetable plant, maize plant, soya bean plant, cotton plant, tobacco plant, rice plant, sugar beet plant, oilseed rape plant or potato plant.

[0014] One preferred embodiment refers to the method described above, characterized in that the use form of the compound of the formula (I) is present in a mixture with at least one mixing partner.

[0015] One preferred embodiment refers to the method described above, characterized in that the Bt toxin of a Bt-plant is encoded by a bt-gene or fragment thereof comprising event MON87701.

[0016] Another aspect refers to a synergistic composition comprising a Bt toxin and a compound of formula (I) as described above. [0017] One preferred embodiment refers to said synergistic composition, characterized in that the Bt toxin is encoded by a cry gene or a cry-gene fragment selected from the group consisting of cryl , cry2, cry3, cry 5 and cry 9.

[0018] One preferred embodiment refers to said synergistic composition, characterized in that the Bt toxin is encoded by a cry gene or a cry-gene fragment selected from the group consisting of especially preferred are crylAb, crylAc, cry3A, cry3B and cry9C.

[0019] One preferred embodiment refers to said synergistic composition, characterized in that the Bt toxin is encoded by a cry gene or a cry-gene fragment selected from the subgroup cryl A, preferably cryl Aa, cryl Ab, cryl Ac or a hybrid thereof (e.g., a hybrif of cryl Ac and cryl Ab).

[0020] One preferred embodiment refers to said synergistic composition, characterized in that the Bt toxin is encoded by a bt-gene or fragment thereof comprising event MON87701.

[0021] A Bt plant, preferably a Bt-soybean plant comprising event MON87701 or a Bt-soybean plant comprising event MON87701 and MON89788, charcterized in that at least 0.00001 g of a compound of formula (I) is attached to it.

[0022] The preferred embodiments may be combined as long as such a combination would not contravene existing natural laws.

Detailed description

[0023] Compounds of the formula (I)

wherein

A represents individually halogen, cyano, nitro, hydroxyl, amino, Ci-Cs alkyl group, substituted Ci- C8 alkyl group having at least one substituent elected from the group consisting of halogen, hydroxy, cyano, nitro, amino, halo C1-C3 alkyl group, C1-C3 alkoxy group, halo C1-C3 alkoxy group, C1-C3 alkylthio group, halo C1-C3 alkylthio group, C1-C3 alkylsulfinyl group, halo C1-C3 alkylsulfinyl group, Ci- C3 alkylsulfonyl group, halo C1-C3 alkylsulfonyl group and C1-C3 alkylthio, C1-C3 alkyl group; further, an arbitrary saturated carbon atom in said optionally substituted Ci-Cs alkyl group; n represents 0, 1 , 2, 3 or 4, preferably 0, 1 or 2;

Ri represents hydrogen, halogen, cyano Ci-Cs alkyl or Ci-Cs haloalkyl;

R2 represents hydrogen, halogen, cyano Ci-Cs alkyl or Ci-Cs haloalkyl;

R3 represents O or S; R represents O or S;

Y represents individually hydrogen, halogen, cyano, nitro, Ci-Ce alkyl group, halo Ci-Ce alkyl group, C2-C6 alkenyl group, halo C2-C6 alkenyl group, C2-C6 alkynyl group, halo C2-C6 alkynyl group, C3-C6 cycloalkyl group, halo C3-C6 cycloalkyl group, Ci-Ce alkoxy group, halo Ci-Ce alkoxy group, Ci- e alkylthio group, halo Ci-Ce alkylthio group, Ci-Ce alkylsulfinyl group, halo Ci-Ce alkylsulfinyl group, Ci-Ce alkylsulfonyl group, or halo Ci-Ce alkylsulfonyl group; m represents 0, 1 , 2, 3, or 4;

X represents a Ci-Cs alkyl group or a substituted Ci-Cs alkyl group having at least one substituent selected from the group consisting of halogen, hydroxy, cyano, nitro, amino, halo C1-C3 alkyl group, Ci- C3 alkoxy group, halo C1-C3 alkoxy group and their insecticidal action are known from the prior art (see, e.g., EP 0 919 542, W0 2004/018410, W0 2010/012442 or WO 2012/034472).

[0024] From these documents, the person skilled in the art will be familiar with processes for preparing and methods for using compounds of the formula (I) and with the action of compounds of the formula (I).

[0025] Preferred sub-groups and compounds of formula (I) mentioned above are listed below. [0026] In a preferred embodiment of the present invention, the compounds of the general formula (I) is represented by compounds of formula (1-1):

wherein

Hal represents F, CI, I or Br; and X' represents Ci-Ce alkyl or substituted Ci-Ce alkyl having at least one substituent selected from the group consisting of halogen, hydroxy, cyano, nitro, amino, halo C1-C3 alkyl group, preferably a Ci-Ce cyanoalkyl;

A' represents C1-C3 alkyl, C1-C3 haloalkyl, halogen, preferably methyl, halomethyl, ethyl or haloethyl, more preferably methyl or ethyl; n represents 0, 1 , 2, 3 or 4, preferably 0, 1 or 2, more preferably 1.

[0027] In a more preferred embodiment of the present invention, a composition comprises at least one compound of the general formula (I) selected from the group consisting of compound (1-2), (1-3), (1-4) or (1-5):

[0028] Even more preferably, a compound of formula (I) is selected from the group consisting of compound (1-2) or compound (1-5).

[0029] In one preferred embodiment, the compound of formula (I) is compound (1-5). [0030] According to the invention, "alkyl" represents straight-chain or branched aliphatic hydrocarbons having 1 to 8, preferably 1 to 6, more preferably 1 to 3, carbon atoms. Suitable alkyl groups are, for example, methyl, ethyl, ^-propyl, z^'-propyl, n-, iso-, sec- or teri-butyl, pentyl or hexyl. The alkyl group may be unsubstituted or is substituted by at least one of the substituents mentioned here.

[0031 ] According to the invention, "halogen" or "Hal" represents fluorine, chlorine, bromine or iodine, preferably fluorine, chlorine or bromine.

[0032] According to the invention, "haloalkyl" represents alkyl groups having up to 8 carbon atoms in which at least one hydrogen atom has been replaced by a halogen. Suitable haloalkyl groups are, for example, CH₂F, CHF₂, CF₃, CF₂C1, CFC1₂, CC1₃, CF₂Br, CF₂CF₃, CFHCF₃, CH₂CF₃, CH₂CH₂F, CH₂CHF₂, CFC1CF₃, CC1₂CF₃, CF₂CH₃, CF₂CH₂F, CF₂CHF₂, CF₂CF₂C1, CF₂CF₂Br, CFHCH₃, CFHCHF₂, CHFCF₃, CHFCF₂C1, CHFCF₂Br, CFC1CF₃, CC1₂CF₃, CF₂CF₂CF₃, CH₂CH₂CH₂F,

CH₂CHFCH₃, CH₂CF₂CF₃, CF₂CH₂CF₃, CF₂CF₂CH₃, CHFCF₂CF₃, CF₂CHFCF₃, CF₂CF₂CHF₂, CF₂CF₂CH₂F, CF₂CF₂CF₂C1, CF₂CF₂CF₂Br, l,2,2,2-tetrafluoro-l-(trifluoromethyl)ethyl, 2,2,2-trifluoro- 1 -(trifluoromethyl)ethyl, pentafluoroethyl, 1 -(difluoromethyl)- 1 ,2,2,2-tetrafluoroethyl, 2-bromo- 1,2,2- trifluoro-l-(trifluoromethyl)ethyl, l-(difluoromethyl)-2,2,2-trifluoroethyl. The haloalkyl group may be unsubstituted or is substituted by at least one of the substituents mentioned here.

[0033] "Production potential" as used herein refers to the yield of a transgenic plant under specific conditions. "Improving the utilization of the production potential of transgenic plants" thus refers to an increase of yield under unfavorable environmental conditions such as use of herbicides, drought stress, cold stress, stress induced by insects, nematodes, or fungis etc. compared to the yoeld of such plants under the same conditions without the use of the compounds of formula (I) as described herein.

[0034] The method can also be used for an increased controll/an increased treatment of pests such as insects and/or nematodes. Thus, the combination of a transgenic plant such as a Bt-plant and a compound of formula (I) can show better treatment/control/combating of insects and/or nematodes compared to the expected effect.

[0035] According to the method proposed according to the invention, transgenic plants, in particular useful plants, are treated with compounds of the formula (I) to increase agricultural productivity and/or to control and/or to combat pests, especially nematodes and insects. Preferably, the invention refers to a method for combating pests by treating transgenic plants, preferably insect-resistant transgenic plant such as Bt-plants or Vip-plants with a compound of formula (I), preferably with a compound of formula (1-5).

[0036] For the purpose of the invention, genetically modified organisms (GMOs), e.g. plants or seeds, are genetically modified plants (or transgenic plants) are plants of which a heterologous gene has been stably integrated into genome. The expression "heterologous gene" essentially means a gene which is provided or assembled outside the plant and when introduced in the nuclear, chloroplastic or mitochondrial genome gives the transformed plant new or improved agronomic or other properties by expressing a protein or polypeptide of interest or by downregulating or silencing other gene(s) which are present in the plant (using for example, antisense technology, cosuppression technology, RNA interference - RNAi - technology or microRNA - miRNA - technology). A heterologous gene that is located in the genome is also called a transgene. A transgene that is defined by its particular location in the plant genome is called a transformation or transgenic event.

[0037] Depending on the plant species or plant cultivars, their location and growth conditions (soils, climate, vegetation period, diet), the treatment according to the invention may also result in superadditive ("synergistic") effects. Thus, for example, reduced application rates and/or a widening of the activity spectrum and/or an increase in the activity of the active compounds and compositions which can be used according to the invention, better plant growth, increased tolerance to high or low temperatures, increased tolerance to drought or to water or soil salt content, increased flowering performance, easier harvesting, accelerated maturation, higher harvest yields, bigger fruits, larger plant height, greener leaf color, earlier flowering, higher quality and/or a higher nutritional value of the harvested products, higher sugar concentration within the fruits, better storage stability, increased combating of pests, especially nematodes and insects and/or processability of the harvested products are possible, which exceed the effects which were actually to be expected.

[0038] At certain application rates, the active compound combinations according to the invention may also have a strengthening effect in plants. Accordingly, they are also suitable for mobilizing the defense system of the plant against attack by unwanted microorganisms. This may, if appropriate, be one of the reasons of the enhanced activity of the combinations according to the invention, for example against fungi. Plant-strengthening (resistance- inducing) substances are to be understood as meaning, in the present context, those substances or combinations of substances which are capable of stimulating the defense system of plants in such a way that, when subsequently inoculated with unwanted microorganisms, the treated plants display a substantial degree of resistance to these microorganisms. In the present case, unwanted microorganisms are to be understood as meaning phytopathogenic fungi, bacteria and viruses. Thus, the substances according to the invention can be employed for protecting plants against attack by the abovementioned pathogens within a certain period of time after the treatment. The period of time within which protection is effected generally extends from 1 to 10 days, preferably 1 to 7 days, after the treatment of the plants with the active compounds.

[0039] Plants and plant cultivars which are preferably to be treated according to the invention include all plants which have genetic modified material which impart particularly advantageous, useful traits to these plants (whether obtained by breeding and/or biotechnological means). [0040] Plants and plant cultivars which are also preferably to be treated according to the invention are resistant against one or more biotic stresses, i.e. said plants show a better defense against animal and microbial pests, such as against nematodes, insects, mites, phytopathogenic fungi, bacteria, viruses and/or viroids.

[0041] Examples of nematode or insect resistant plants are described in e.g. U.S. Patent Applications 11/765,491, 11/765,494, 10/926,819, 10/782,020, 12/032,479, 10/783,417, 10/782,096, 11/657,964, 12/192,904, 11/396,808, 12/166,253, 12/166,239, 12/166,124, 12/166,209, 11/762,886, 12/364,335, 11/763,947, 12/252,453, 12/209,354, 12/491,396, 12/497,221, 12/644,632, 12/646,004, 12/701,058, 12/718,059, 12/721,595, 12/638,591, and in WO 11/002992, WO 11/014749, WO 11/103247, WO 11/103248, WO 12/135436, WO 12/135501. [0042] Examples of plants resistant to other types of pathogens are described in e.g. WO13/050410.

[0043] Plants and plant cultivars which may also be treated according to the invention are those plants which are resistant to one or more abiotic stresses. Abiotic stress conditions may include, for example, drought, cold temperature exposure, heat exposure, osmotic stress, flooding, increased soil salinity, increased mineral exposure, ozone exposure, high light exposure, limited availability of nitrogen nutrients, limited availability of phosphorus nutrients, shade avoidance.

[0044] Plants and plant cultivars which may also be treated according to the invention, are those plants characterized by enhanced yield characteristics. Increased yield in said plants can be the result of, for example, improved plant physiology, growth and development, such as water use efficiency, water retention efficiency, improved nitrogen use, enhanced carbon assimilation, improved photosynthesis, increased germination efficiency, inproved combating of insects and accelerated maturation. Yield can furthermore be affected by improved plant architecture (under stress and non-stress conditions), including but not limited to, early flowering, flowering control for hybrid seed production, seedling vigor, plant size, internode number and distance, root growth, seed size, fruit size, pod size, pod or ear number, seed number per pod or ear, seed mass, enhanced seed filling, reduced seed dispersal, reduced pod dehiscence and lodging resistance. Further yield traits include seed composition, such as carbohydrate content, protein content, oil content and composition, nutritional value, reduction in anti-nutritional compounds, improved processability and better storage stability.

[0045] Examples of plants with the above-mentioned traits are non-exhaustively listed in Table A. Table A:

Event Company Description Crop Patent Ref

Glyphosate tolerance derived by inserting a

Agrostis

modified 5-enolpyruvylshikimate-3-

ASR36 Scotts stolonifera US 2006- phosphate synthase (EPSPS) encoding gene

8 Seeds Creeping 162007 from Agrobacterium tumefaciens, parent

Bentgrass

line B99061

Glyphosate herbicide tolerant canola

produced by inserting genes encoding the

enzymes 5-enolypyruvylshikimate-3- Brassica

Monsanto

GT200 phosphate synthase (EPSPS) from the CP4 napus (Argent

Company

strain of Agrobacterium tumefaciens and ine Canola)

glyphosate oxidase from Ochrobactrum

anthropi.

Delayed softening tomatoes produced by

inserting a truncated version of the

Lycopersicon

B, Da, Zeneca polygalacturonase (PG) encoding gene in the

esculentum (T

F Seeds sense or anti-sense orientation in order to

omato)

reduce expression of the endogenous PG

gene, and thus reduce pectin degradation.

Delayed softening tomatoes produced by

inserting an additional copy of the

Lycopersicon

FLAVR Calgene polygalacturonase (PG) encoding gene in the

esculentum (T

SAVR Inc. anti-sense orientation in order to reduce

omato)

expression of the endogenous PG gene and

thus reduce pectin degradation.

Monsanto Glyphosate herbicide tolerant alfalfa

Company (lucerne) produced by inserting a gene

Medicago

J101, and Forage encoding the enzyme 5- sativa (Alfalfa

J163 Genetics enolypyruvylshikimate-3 -phosphate

Internation synthase (EPSPS) from the CP4 strain of )

al Agrobacterium tumefaciens.

Societe

National

Tolerance to the herbicides bromoxynil and Nicotiana

C/F/93/ d'Exploitat

ioxynil by incorporation of the nitrilase gene tabacum

08-02 ion des

from Klebsiella pneumoniae. L. (Tobacco)

Tabacs et

Allumettes

Reduced nicotine content through

introduction of a second copy of the tobacco

Vector quinolinic acid phosphoribosyltransferase Nicotiana

Vector

Tobacco (QTPase) in the antisense orientation. The tabacum

21-41

Inc. NPTII encoding gene from E. coli was L. (Tobacco)

introduced as a selectable marker to identify

transformants.

United

States

Departmen

t of Plum pox virus (PPV) resistant plum tree

Prunus Agricultur produced through Agrobacterium-mediated

C5 domestica e - transformation with a coat protein (CP) gene

(Plum)

Agricultur from the virus.

al

Research

Service

ATBTO

4-6,

ATBTO

4-27,

ATBTO

4-30,

Colorado potato beetle resistant potatoes Solanum ATBTO Monsanto

produced by inserting the cry3A gene from tuberosum

4-31, Company

Bacillus thuringiensis (subsp. Tenebrionis). L. (Potato) ATBTO

4-36,

SPBTO

2-5,

SPBTO

2-7

BT6,

BTIO,

BT12, Colorado potato beetle resistant potatoes Solanum

Monsanto

BT16, produced by inserting the cry3A gene from tuberosum Company

BT17, Bacillus thuringiensis (subsp. Tenebrionis). L. (Potato) BT18,

BT23

RBMT

Colorado potato beetle and potato virus Y

15-101,

(PVY) resistant potatoes produced by Solanum SEMT1 Monsanto

inserting the cry3A gene from Bacillus tuberosum

5-02, Company

thuringiensis (subsp. Tenebrionis) and the L. (Potato) SEMT1

coat protein encoding gene from PVY.

5-15

RBMT

Colorado potato beetle and potato leafroll

21-129,

virus (PLRV) resistant potatoes produced by Solanum RBMT Monsanto

inserting the cry3A gene from Bacillus tuberosum

21- 350, Company

thuringiensis (subsp. Tenebrionis) and the L. (Potato) RBMT

replicase encoding gene from PLRV.

22- 082

Introduction of the PPT-acetyltransferase

(PAT) encoding gene from Streptomyces

Aventis Brassica viridochromogenes, an aerobic soil bacteria.

HCN10 CropScien napus (Argent

PPT normally acts to inhibit glutamine

ce ine Canola) synthetase, causing a fatal accumulation of

ammonia. Acetylated PPT is inactive.

Selection for a mutagenized version of the

Triticum

AP205 enzyme acetohydroxyacid synthase (AHAS),

BASF Inc. aestivum (Wh CL also known as acetolactate synthase (ALS)

eat) or acetolactate pyruvate- lyase.

Stacked insect resistant and herbicide

tolerant maize produced by conventional

cross breeding of parental lines BT11

(OECD unique identifier: SYN-BT011-1),

MIR604 (OECD unique identifier: SYN-

IR605-5) and GA21 (OECD unique

identifier: MON-00021-9). Resistance to

the European Corn Borer and tolerance to

BT11 x the herbicide glufosinate ammonium

MIR60 Syngenta (Liberty) is derived from BT11, which Zea mays

4 x Seeds, Inc. contains the crylAb gene from Bacillus L. (Maize)

GA21 thuringiensis subsp. kurstaki, and the

phosphinothricin N-acetyltransferase (PAT)

encoding gene from S. viridochromogenes.

Corn rootworm-resistance is derived from

MIR604 which contains the mcry3A gene

from Bacillus thuringiensis. Tolerance to

glyphosate herbcicide is derived from GA21

which contains a a modified EPSPS gene

from maize.

Insect-resistant and glufosinate ammonium

herbicide tolerant maize developed by

Aventis

CBH- inserting genes encoding Cry9C protein Zea mays

CropScien

351 from Bacillus thuringiensis subsp tolworthi L. (Maize)

ce

and phosphinothricin acetyltransferase

(PAT) from Streptomyces hygroscopicus.

Lepidopteran insect resistant and glufosinate

ammonium herbicide-tolerant maize variety

DAS- DOW

produced by inserting the cry IF gene from Zea mays

06275- AgroScien

Bacillus thuringiensis var aizawai and the L. (Maize)

8 ces LLC

phosphinothricin acetyltransferase (PAT)

from Streptomyces hygroscopicus.

DOW

AgroScien Corn rootworm-resistant maize produced by

ces LLC inserting the cry34Abl and cry35Abl genes

DAS- US 2006- and from Bacillus thuringiensis strain PS149B1. Zea mays

59122- 070139, US

Pioneer The PAT encoding gene from Streptomyces L. (Maize)

7 2011030086 Hi-Bred viridochromogenes was introduced as a

Internation selectable marker; US 2006-070139

al Inc.

Stacked insect resistant and herbicide

tolerant maize produced by conventional

DOW

cross breeding of parental lines DAS-59122- AgroScien

7 (OECD unique identifier: DAS-59122-7)

DAS- ces LLC

with NK603 (OECD unique identifier:

59122- and Zea mays

MON-00603-6). Corn rootworm-resistance

7 x Pioneer L. (Maize)

is derived from DAS-59122-7 which

NK603 Hi-Bred

contains the cry34Abl and cry35Abl genes

Internation

from Bacillus thuringiensis strain PS149B1.

al Inc.

Tolerance to glyphosate herbcicide is

derived from NK603.

Corn rootworm-resistant maize produced by

inserting the cry3Bbl gene from Bacillus

thuringiensis subspecies kumamotoensis

MON8 Monsanto strain EG4691. Glyphosate tolerance derived Zea mays WO 8017 Company by inserting a 5-enolpyruvylshikimate-3- L. (Maize) 2009111263 phosphate synthase (EPSPS) encoding gene

from Agrobacterium tumefaciens strain

CP4; WO2005059103

Maize event expressing two different

insecticidal proteins from Bacillus

MON8 Monsanto thuringiensis providing resistance to number Zea mays WO 9034 Company of lepidopteran pests; nsect resistance L. (Maize) 2005/059103

(Lepidoptera -CrylA.105- Cry2Ab); WO

2007140256

Stacked insect resistant and glyphosate

tolerant maize derived from conventional

cross-breeding of the parental lines

MON89034 (OECD identifier: MON- 89034-3) and MON88017 (OECD

MON8 identifier:MON-88017-3). Resistance to

9034 x Monsanto Lepiopteran insects is derived from two Zea mays WO MON8 Company crygenes present in MON89043. Corn L. (Maize) 2007140256 8017 rootworm resistance is derived from a single

cry genes and glyphosate tolerance is

derived from the 5-enolpyruvylshikimate-3- phosphate synthase (EPSPS) encoding gene

from Agrobacterium tumefaciens present in

MON88017.

MS- AVENTIS Brassica

BN1/R CROPSCI Male sterility/restoration; WO 01/41558 napus (Argent

F-BN1 ENCE NV ine Canola)

Stacked insect resistant and herbicide

tolerant maize produced by conventional

cross breeding of parental lines MON89034

MON8 (OECD identifier: MON-89034-3) with

Monsanto Zea mays

9034 x NK603 (OECD unique identifier: MON- WO 01/41558

Company L. (Maize)

NK603 00603-6). Resistance to Lepiopteran

insects is derived from two crygenes present

in MON89043. Tolerance to glyphosate

herbcicide is derived from NK603.

MON8

Stacked insect resistant and herbicide

9034 x

tolerant maize produced by conventional

TC1507

cross breeding of parental lines:

X

Monsanto MON89034, TC1507, MON88017, and Zea mays

MON8

Company DAS-59122. Resistance to the above-ground L. (Maize)

8017 x

and below-ground insect pests and tolerance

DAS- to glyphosate and glufosinate-ammonium

59122- containing herbicides.

7

MON- Stacked insect resistant and herbicide

00603 tolerant corn hybrid derived from

-6 x Monsanto conventional cross-breeding of the parental Zea mays

MON- Company lines NK603 (OECD identifier: MON- L. (Maize)

00810 00603-6) and MON810 (OECD identifier:

-6 MON-00810-6).

Male sterility was via insertion of the

RM3-3, barnase ribonuclease gene from Bacillus Cichorium

Bejo WO 2004- RM3-4, amyloliquefaciens; PPT resistance was via intybus (Chic

Zaden BV 074492 RM3-6 the bar gene from S. hygroscopicus, which ory)

encodes the PAT enzyme.

PIONEER

HI-BRED

INTERNA

TIONAL

DP- INC, E.I

Glyphosate tolerance / ALS inhibitor Zea mays

098140 DU PONT

tolerance L. (Maize)

-6 DE

NEMOUR S AND COMPAN Y

Reduced accumulation of S- adenosylmethionine (SAM), and WO

Agritope Cucumis

A, B consequently reduced ethylene synthesis, by 2008/112019,

Inc. melo (Melon)

introduction of the gene encoding S- US2010240059 adenosylmethionine hydrolase.

Cucumber mosiac virus (CMV), zucchini

As grow

yellows mosaic (ZYMV) and watermelon

(USA);

mosaic virus (WMV) 2 resistant squash (

Seminis Cucurbita

CZW-3 Curcurbita pepo) produced by inserting the

Vegetable pepo (Squash)

coat protein (CP) encoding sequences from

Inc.

each of these plant viruses into the host

(Canada)

genome.

Upjohn Zucchini yellows mosaic (ZYMV) and

(USA); watermelon mosaic virus (WMV) 2 resistant

Seminis squash ( Curcurbita pepo) produced by Cucurbita

ZW20

Vegetable inserting the coat protein (CP) encoding pepo (Squash)

Inc. sequences from each of these plant

(Canada) potyviruses into the host genome.

Delayed senescence and sulfonylurea

herbicide tolerant carnations produced by

inserting a truncated copy of the carnation

aminocyclopropane cyclase (ACC) synthase

encoding gene in order to suppress

Dianthus

Florigene expression of the endogenous unmodified

66 caryophyllus (

Pty Ltd. gene, which is required for normal ethylene

Carnation)

biosynthesis. Tolerance to sulfonyl urea

herbicides was via the introduction of a

chlorsulfuron tolerant version of the

acetolactate synthase (ALS) encoding gene

from tobacco.

Modified colour and sulfonylurea herbicide

tolerant carnations produced by inserting

two anthocyanin biosynthetic genes whose

expression results in a violet/mauve Dianthus

4, 11, Florigene

colouration.Tolerance to sulfonyl urea caryophyllus (

15, 16 Pty Ltd.

herbicides was via the introduction of a Carnation)

chlorsulfuron tolerant version of the

acetolactate synthase (ALS) encoding gene

from tobacco.

DuPont

Gossypium

Canada Introduction of a variant form of acetolactate WO

19-51A hirsutum

Agricultur synthase (ALS). 2006/128570

L. (Cotton)

al Products

Insect-resistant cotton produced by inserting

the cry IF gene from Bacillus

DOW Gossypium

281-24- thuringiensisvar. aizawai. The PAT

AgroScien hirsutum

236 encoding gene from Streptomyces

ces LLC L. (Cotton)

viridochromogenes was introduced as a

selectable marker.

SES

Beta vulgaris

T227-1 EUROPE Glyphosate tolerance; US 2004-117870

(sugar beet)

N.V./S.A

Insect-resistant cotton produced by inserting

the cry 1 Ac gene from Bacillus

DOW Gossypium

3006- thuringiensissubsp. kurstaki. The PAT US 2004- AgroScien hirsutum

210-23 encoding gene from Streptomyces 117870 ces LLC L. (Cotton)

viridochromogenes was introduced as a

selectable marker.

Insect-resistant and bromoxynil herbicide

tolerant cotton produced by inserting the Gossypium

31807/3 Calgene

crylAc gene from Bacillus thuringiensis and hirsutum 1808 Inc.

a nitrilase encoding gene from Klebsiella L. (Cotton)

pneumoniae.

Bromoxynil herbicide tolerant cotton Gossypium

Calgene

BXN produced by inserting a nitrilase encoding hirsutum

Inc.

gene from Klebsiella pneumoniae. L. (Cotton)

Syngenta Gossypium

CE43- Insect resistance (Cryl Ab); WO

Participati hirsutum

67B 2006/128573

ons AG L. (Cotton)

WO

Syngenta Gossypium

CE44- Insect resistance (Cryl Ab); WO 2006/128573, Participati hirsutum

69D 2006/128571 US ons AG L. (Cotton)

2011020828

Syngenta Gossypium

CE46- Insect resistance (Cryl Ab); WO WO Participati hirsutum

02A 2006/128572 2006/128571 ons AG L. (Cotton)

Insect-resistant cotton produced by inserting

the vip3A(a) gene from Bacillus Gossypium

Syngenta WO

Cotl02 thuringiensisAB88. The APH4 encoding hirsutum

Seeds, Inc. 2006/128572 gene from E. coli was introduced as a L. (Cotton)

selectable marker.; US 2006-130175

US 2006-

Gossypium 130175,

COT20 Syngenta

Insect resistance (VIP3A); US2009181399 hirsutum WO200403998 2 Seeds, Inc.

L. (Cotton) 6, US

2010298553

Insect-resistant cotton produced by inserting

a full-length crylAb gene from Bacillus Gossypium

Syngenta

Cot67B thuringiensis. The APH4 encoding gene hirsutum - Seeds, Inc.

from E. coli was introduced as a selectable L. (Cotton)

marker. Monsanto High laurate (12:0) and myristate (14:0)

23-18- Brassica

Company canola produced by inserting a thioesterase

17, 23- napus (Argent

(formerly encoding gene from the California bay laurel

198 ine Canola)

Calgene) (Umbellularia californica).

DAS-

WideStrike™, a stacked insect-resistant

21023-

DOW cotton derived from conventional crossGossypium

5 x

AgroScien breeding of parental lines 3006-210-23 hirsutum

DAS- ces LLC (OECD identifier: DAS-21023-5) and 281- L. (Cotton)

24236- 24-236 (OECD identifier: DAS-24236-5).

5

DAS- DOW

21023- AgroScien Stacked insect-resistant and glyphosate-

5 x ces LLC tolerant cotton derived from conventional

Gossypium

DAS- and cross-breeding of WideStrike cotton (OECD

hirsutum

24236- Pioneer identifier: DAS-21023-5 x DAS-24236-5)

L. (Cotton)

5 x Hi-Bred with MON88913, known as RoundupReady

MON8 Internation Flex (OECD identifier: MON-88913-8).

8913 al Inc.

DAS- 21023- WideStrike™/Roundup Ready® cotton, a

5 x stacked insect-resistant and glyphosate- DAS- DOW tolerant cotton derived from conventional Gossypium

24236- AgroScien cross-breeding of WideStrike cotton (OECD hirsutum

5 x ces LLC identifier: DAS-21023-5 x DAS-24236-5) L. (Cotton)

MON- with MON1445 (OECD identifier: MON- 01445- 01445-2).

2

BAYER Gossypium

EE- BIOSCIE Glyphosate tolerance; WO 2007/017186 hirsutum

GH3

NCE NV L. (Cotton)

BAYER Gossypium

EE- Insect resistance (Cryl Ab); WO WO BIOSCIE hirsutum

GH5 2008/122406 2007/017186 NCE NV L. (Cotton)

BAYER Gossypium

EE- WO BIOSCIE Insect resistance (cry2Ae); WO2008151780 hirsutum

GH6 2008/122406 NCE NV L. (Cotton)

event DOW Gossypium WO200815178 281-24- AgroScien Insect resistance (CrylF); WO 2005/103266 hirsutum o, 236 ces LLC L. (Cotton) US2010218281

JK Agri Insect-resistant cotton produced by inserting Gossypium

WO

Event- 1 Genetics the cryl Ac gene from Bacillus thuringiensis hirsutum

2005/103266 Ltd (India) subsp. kurstaki HD-73 (B.t.k.). L. (Cotton)

event30 DOW Gossypium

Insect resistance (Cryl Ac); WO

06-210- AgroScien hirsutum

2005/103266

23 ces LLC L. (Cotton)

Bayer

CropScien

Glyphosate herbicide tolerant cotton

ce Gossypium

GBH61 produced by inserting 2mepsps gene into WO

(Aventis hirsutum

4 variety Coker312 by Agrobacterium under 2005/103266 CropScien L. (Cotton)

the control of Ph4a748At and TPotpC

ce(AgrEvo

))

Introduction of a gene sequence encoding

Lycopersicon

Agritope the enzyme S-adenosylmethionine hydrolase

35 1 N esculentum (T

Inc. that metabolizes the precursor of the fruit

omato)

ripening hormone ethylene

BASF AGROCH

Glycine max

127 EMICAL ALS/AHAS inhibitor-tolerance

L. (Soybean)

PRODUC TS B.V.

Syngenta

Zea mays WO201008082

5307 Participati Insect (corn rootworm) resistance (FR8a)

L. (Maize) 9 ons AG

MONSAN

TO

Oryza WO201007781

17053 TECHNO Glyphosate tolerance

sativa (Rice) 6 LOGY LLC

BAYER

Oryza WO201011773

17314 BIOSCIE Glyphosate tolerance

sativa (Rice) 7 NCE NV

Pioneer

3560.4. Hi-Bred Glycine max WO201011773

Glyphosate/ALS inhibitor-tolerance

3.5 Internation L. (Soybean) 5

al Inc.

BAYER WO

A2704- Glycine max

BIOSCIE Glufosinate tolerance 2008002872, 12 L. (Soybean)

NCE NV US2010184079

BAYER

A5547- Glycine max WO

BIOSCIE Glufosinate tolerance

35 L. (Soybean) 2006/108674 NCE NV

Syngenta

GM Beet Necrotic Yellow Vein Virus (BNYW) Beta vulgaris WO Participati

RZ13 resistance (sugar beet) 2006/108675 ons AG

Syngenta

JOPLI disease (fungal) resistance (trichothecene 3- WO201007621 Participati Wheat

Nl O-acetyltransferase) 2

ons AG

BAYER Gossypium

LLcotto US

BIOSCIE Glufosinate resistance hirsutum

n25 2008064032 NCE NV L. (Cotton)

AVENTIS CROPSCl Brassica (A WO

MS-B2 Male sterility

ENCE genome) 2003013224 N.V.

AVENTIS

MS- CROPSCl Brassica

BN1/R Male sterility/restoration WO 01/31042

ENCE (napus)

F-BN1

N.V.

MONSAN

TO

Brassica

RT73 TECHNO Glyphosate resistance WO 01/41558

(napus)

LOGY LLC CHINA Transgenic rice Kefeng 6 is a transformation

Kefeng NAT event containing two insect-resistant genes, Oryza

WO 02/36831 No. 6 RICE RES cry 1 Ac and SCK (modified CpTI gene) in sativa (Rice)

INST China.

1) MS45: anther-specific 5126 (Zea mays)

promoter > fertility restoration Ms45 (Zea

mays) coding sequence > fertility restoration

Ms45 (Zea mays) 3 '-untranslated region 2)

ZM-AAl : polygalacturonase 47 (Zea mays)

E6611.

promoter > brittle- 1 (Zea mays) chloroplast

32.1.38 Pioneer

transit peptide > alpha-amylase-1 (Zea

/ DP- Hi-Bred zea mays

mays) truncated coding sequence > >In2-l CN 101824411 32138- Internation L. (Maize)

(Zea mays) 3 '-untranslated region 3)

1 / al Inc.

DSRED2: 35S (Cauliflower Mosaic Virus)

32138

enhancer > lipid transfer protein-2

(Hordeum vulgare) promoter > red

fluorescent protein (Dicosoma sp.) variant

coding sequence > protein inhibitor II

(Solanum tuberosum) 3 '-untranslated region

RB7 MARv3>zmUbiquitin 1

promoter>aadl>zmPER5 3'UTR>RB 7

WO

DAS- DOW MARv4. The aad-1 gene confers tolerance

Zea mays 2009103049,

40278- AgroScien to 2,4- dichlorophenoxyacetic acid and

L. (Maize) MX

9 ces LLC aryloxyphenoxypropionate (commonly

2010008977 referred to as "fop" herbicides such as

quizalofop) herbicides

1) CRY3A: metallotionin-like gene (Zea

mays) promoter > delta-endotoxin cry3a

(Bacillus thuringiensis subsp. tenebrionis )

coding sequence, modified to include a

cathepsin-G protease recognition site and

Syngenta maize codon optimized > nopaline synthase

MIR60 Zea mays WO 201102246 Participati (Agrobacterium tumefaciens) 3 '-untranslated

4 L. (Maize) 9

ons AG region 2) PMI: polyubiquitin (Zea mays)

promoter (incl. first intron) > mannose-6- phosphate isomerase (Escherichia coli)

coding sequence > nopaline synthase

(Agrobacterium tumefaciens) 3 '-untranslated

region

Dicamba herbicide tolerance, transformation

vector PV- GMHT4355 1) DMO: full length

transcript (Peanut Chlorotic Streak Virus)

promoter > tobacco Etch Virus leader >

ribulose 1,5-biphosphate carboxylase small US

MONSAN

subunit (Pisum sativum) chloroplast transit 2005216970, TO

MON peptide > dicamba mono-oxygenase Glycine max US

TECHNO

87708 (Stenotrophomonas maltophilia) coding L. (Soybean) 2008167456,

LOGY

sequence > ribulose- 1,5-bisphosphate US

LLC

carboxylase small subunit E9 (Pisum 2011111420 sativum) 3 '-untranslated region. A CP4

epsps chimeric gene contained within a

second T-DNA on the transformation vector

used was segregated away. The transgene insert and expression cassette

of MON 87427 comprises the promoter and

leader from the cauliflower mosaic virus

(CaMV) 35 S containing a duplicated

enhancer region (P-e35S); operably linked to

a DNA leader derived from the first intron

from the maize heat shock protein 70 gene

MONSAN

(I- HSP70); operably linked to a DNA

TO

MON molecule encoding an N-terminal Zea mays WO 201103470

TECHNO

87427 chloroplast transit peptide from the shkG L. (Maize) 4

LOGY

gene from Arabidopsis thaliana EPSPS (Ts-

LLC CTP2); operably linked to a DNA molecule

derived from the aroA gene from the

Agrobacterium sp. strain CP4 and encoding

the CP4 EPSPS protein; operably linked to a

3' UTR DNA molecule derived from the

nopaline synthase (T-NOS) gene from

Agrobacterium tumefaciens .

1) Ph4a748 ABBC: sequence including the

promoter region of the histone H4 gene of

Arabidopsis thaliana, containing an internal

duplication>5'tev: sequence including the

leader sequence of the tobacco etch

virus>TPotp Y: coding sequence of an

optimized transit peptide derivative (position

55 changed into Tyrosine), containing

sequence of the RuBisCO small subunit

genes of Zea mays (corn) and Helianthus

annuus (sunflower)>hppdPf W336: the

coding sequence of the 4- hydroxyphenylpyruvate dioxygenase of

Pseudomonas fluorescens strain A32

BAYER

modified by the replacement of the amino

BIOSCIE

acid Glycine 336 with a Tryptophane^ 'nos:

EE- NCE NV

sequence including the 3' untranslated Glycine max WO

GM3 / [BE]; MS

region of the nopaline synthase gene from L. (Soybean) 2011062904 FG72 TECHNO

the T-DNA of pTiT37 of Agrobacterium

LOGIES

tumefaciens. 2) Ph4a748: sequence

LLC [US]

including the promoter region of the histone

H4 gene of Arabidopsis thaliana>intronl

h3At: first intron of gene II of the histone

H3.III variant of Arabidopsis thaliana

>TPotp C: coding sequence of the optimized

transit peptide, containing sequence of the

RuBisCO small subunit genes of Zea mays

(corn) and Helianthus annuus

(sunflower)>2mepsps: the coding sequence

of the double-mutant 5-enol- pyruvylshikimate-3 -phosphate synthase

gene of Zea mays>3 'histonAt: sequence

including the 3 ' untranslated region of the

histone H4 gene of Arabidopsis thaliana A novel aad-12 transformation event for

herbicide tolerance in soybean plants - referred to herein as pDAB4468-0416. The

aad-12 gene (originally from Delftia

416 / DOW acidovorans) encodes the aryloxyalkanoate

pDAB4 AGROSCI dioxygenase (AAD-12) protein. The trait Glycine max WO 201106341 468- ENCES confers tolerance to 2,4- L. (Soybean) 1

0416 LLC dichlorophenoxyacetic acid, for example,

and to pyridyloxyacetate herbicides. The

aad-12 gene, itself, for herbicide tolerance in

plants was first disclosed in WO

2007/053482.

Pioneer crylF, cry34Abl, cry35Abl, and pat:

DP- Hi-Bred resistance to certain lepidopteran and Zea mays WO

004114

Internation coleopteran pests, as well as tolerance to L. (Maize) 2011066384

-3

al Inc. phosphinothricin.

Pioneer CrylF, cry34Abl, cry35Abl, pat: resistance

DP- Hi-Bred to certain lepidopteran and coleopteran Zea mays US 201115452 032316

Internation pests, as well as tolerance to L. (Maize) 3

al Inc. phosphinothricin

Pioneer CrylF, cry34Abl, cry35Abl, pat: resistance

DP- Hi-Bred to certain lepidopteran and coleopteran Zea mays US 201115452 040416

Internation pests, as well as tolerance to L. (Maize) 4

-8 a

al Inc. phosphinothricin

Pioneer CrylF, cry34Abl, cry35Abl, pat: resistance US2011015452

DP- Hi-Bred to certain lepidopteran and coleopteran Zea mays 5

043A47

Internation pests, as well as tolerance to L. (Maize) US2011015452

-3

al Inc. phosphinothricin 6

PIONEER

The invention provides DNA compositions

HI-BRED

that relate to transgenic insect resistant

INTERNA

maize plants. Also provided are assays for

TIONAL,

detecting the presence of the maize DP-

DP- INC. / E.I.

004114-3 event based on the DNA sequence WO2011/08462

004114 DU PONT maize

of the recombinant construct inserted into 1A1

-3 DE

the maize genome and the DNA sequences

NEMOUR

flanking the insertion site. Kits and

S AND

conditions useful in conducting the assays

COMPAN

are provided.

Y

PIONEER

The invention provides DNA compositions

HI-BRED

that relate to transgenic insect resistant

INTERNA

maize plants. Also provided are assays for

TIONAL,

detecting the presence of the maize DP-

DP- INC. / E.I.

032316-8 event based on the DNA sequence WO2011/08463 032316 DU PONT maize

of the recombinant construct inserted into 2

DE

the maize genome and the DNA sequences

NEMOUR

flanking the insertion site. Kits and

S AND

conditions useful in conducting the assays

COMPAN

are provided.

Y The invention provides plants comprising

transgenic event MON 88302 that exhibit

tolerance to glyphosate herbicide. The

MONSAN

invention also provides seeds, plant parts,

MON- TO

cells, commodity products, and methods WO2011/15318 88302- TECHNO brassica

related to the event. The invention also 6

9 LOGY

provides DNA molecules that are unique to

LLC

the event and were created by the insertion

of transgenic DNA into the genome of a

Brassica napus plant.

SYNGEN Soybean plants comprising event

SYN- TA SYHT0H2, methods of detecting and using

WO2012/08254

000H2- PARTICIP the same, and soybean plants comprising a soybean

8A2

5 ATIONS heterologous insert at the same site as

AG SYHT0H2.

This invention relates to soybean event

pDAB8291.45.36.2, which includes a novel

expression cassette comprising multiple

traits conferring resistance to glyphosate,

aryloxyalkanoate, and glufosinate

herbicides. This invention also relates in part

to methods of controlling resistant weeds,

plant breeding, and herbicide tolerant plants.

In some embodiments, the event sequence

DOW

can be "stacked" with other traits, including,

AGROSCl

for example, other herbicide tolerance

DAS- ENCES

gene(s) and/or insect-inhibitory proteins. WO2012/07542

14536- LLC; MS soybean

This invention further relates in part to 9A1

7 TECHNO

detection methods, including endpoint

LOGIES

TaqMan PCR assays, for the detection of

LLC

Event pDAB8291.45.36.2 in soybeans and

related plant material. Some embodiments

can perform high throughput zygosity

analysis of plant material and other

embodiments can be used to uniquely

identify the zygosity of and breed soybean

lines comprising the event of the subject

invention. Kits and conditions useful in

conducting these assays are also provided.

This invention relates in part to soybean

event pDAB8264.44.06.1 and includes a

novel expression cassettes and transgenic

inserts comprising multiple traits conferring

resistance to glyphosate, aryloxyalkanoate,

and glufosinate herbicides. This invention

also relates in part to methods of controlling

resistant weeds, plant breeding and herbicide

tolerant plants. In some embodiments, the

DOW

event sequence can be "stacked" with other

AGROSCl

traits, including, for example, other

DAS- ENCES

herbicide tolerance gene(s) and/or insect- WO2012/07542

44406- LLC; MS soybean

inhibitory proteins. This invention further 6A1

6 TECHNO

relates in part to endpoint TaqMan PCR

LOGIES

assays for the detection of Event

LLC

pDAB8264.44.06.1 in soybeans and related

plant material. Some embodiments can

perform high throughput zygosity analysis

of plant material and other embodiments can

be used to uniquely identify the zygosity of

and breed soybean lines comprising the

event of the subject invention. Kits and

conditions useful in conducting these assays

are also provided.

The present invention provides a transgenic

soybean comprising event MON87712 that

exhibits increased yield. The invention also

provides cells, plant parts, seeds, plants,

commodity products related to the event,

MONSAN and DNA molecules that are unique to the

MON- TO event and were created by the insertion of

WO2012/05119 87712- TECHNO transgenic DNA into the genome of a soybean

9A2

4 LOGY soybean plant. The invention further

LLC provides methods for detecting the presence

of said soybean event nucleotide sequences

in a sample, probes and primers for use in

detecting nucleotide sequences that are

diagnostic for the presence of said soybean

event.

This invention relates to soybean event

pDAB4472-1606 (Event 1606). This

invention includes a novel aad-12

transformation event in soybean plants

comprising a polynucleotide sequence, as

DOW described herein, inserted into a specific site

DAS- AGROSCl within the genome of a soybean cell. This WO2012/03379

21606- soybean

ENCES invention also relates in part to plant 4A2

3

LLC breeding and herbicide tolerant plants. In

some embodiments, said event /

polynucleotide sequence can be "stacked"

with other traits, including, for example,

other herbicide tolerance gene(s) and/or

insect-inhibitory proteins. Compositions and methods related to

transgenic glyphosate tolerant Brassica

plants are provided. Specifically, the present

invention provides Brassica plants having a

DP-061061-7 event which imparts tolerance

to glyphosate. The Brassica plant harboring

the DP-061061-7 event at the recited

chromosomal location comprises

PIONEER

genomic/transgene junctions within SEQ ID

DP- HI-BRED

NO: 2 or with genomic/transgene junctions WO201204926

061061 INTERNA Brassica

as set forth in SEQ ID NO: 12 and/or 13. 8A1

-7 TIONAL

The characterization of the genomic

INC.

insertion site of events provides for an

enhanced breeding efficiency and enables

the use of molecular markers to track the

transgene insert in the breeding populations

and progeny thereof. Various methods and

compositions for the identification,

detection, and use of the events are

provided.

Compositions and methods related to

transgenic glyphosate tolerant Brassica

plants are provided. Specifically, the present

invention provides Brassica plants having a

DP-073496-4 event which imparts tolerance

to glyphosate. The Brassica plant harboring

the DP-073496-4 event at the recited

PIONEER chromosomal location comprises

DP- HI-BRED genomic/transgene junctions within SEQ ID

WO201204966

073496 INTERNA NO: 2 or with genomic/transgene junctions Brassica

1A1

-4 TIONAL as set forth in SEQ ID NO: 12 and/or 13.

INC. The characterization of the genomic

insertion site of the event provides for an

enhanced breeding efficiency and enables

the use of molecular markers to track the

transgene insert in the breeding populations

and progeny thereof. Various methods and

compositions for the identification,

detection, and use of the event are provided.

This invention relates in part to soybean

event pDAB8264.44.06.1 and includes a

novel expression cassettes and transgenic

inserts comprising multiple traits conferring

resistance to glyphosate, aryloxyalkanoate,

and glufosinate herbicides. This invention

also relates in part to methods of controlling

resistant weeds, plant breeding and herbicide

tolerant plants. In some embodiments, the

DOW

event sequence can be "stacked" with other

AGROSCl

traits, including, for example, other

ENCES

8264.44 herbicide tolerance gene(s) and/or insect- WO201205246

LLC; MS Soybean

.06.1 inhibitory proteins. This invention further 8A2

TECHNO

relates in part to endpoint TaqMan PCR

LOGIES

assays for the detection of Event

LLC

pDAB8264.44.06.1 in soybeans and related

plant material. Some embodiments can

perform high throughput zygosity analysis

of plant material and other embodiments can

be used to uniquely identify the zygosity of

and breed soybean lines comprising the

event of the subject invention. Kits and

conditions useful in conducting these assays

are also provided.

This invention relates to soybean event

pDAB8291.45.36.2, which includes a novel

expression cassette comprising multiple

traits conferring resistance to glyphosate,

aryloxyalkanoate, and glufosinate

herbicides. This invention also relates in part

to methods of controlling resistant weeds,

plant breeding, and herbicide tolerant plants.

In some embodiments, the event sequence

DOW

can be "stacked" with other traits, including,

AGROSCl

for example, other herbicide tolerance

ENCES

8291.45 gene(s) and/or insect-inhibitory proteins. WO201205598

LLC; MS Soybean

.36.2 This invention further relates in part to 2A2

TECHNO

detection methods, including endpoint

LOGIES

TaqMan PCR assays, for the detection of

LLC

Event pDAB8291.45.36.2 in soybeans and

related plant material. Some embodiments

can perform high throughput zygosity

analysis of plant material and other

embodiments can be used to uniquely

identify the zygosity of and breed soybean

lines comprising the event of the subject

invention. Kits and conditions useful in

conducting these assays are also provided.

SYHTO SYNGEN Soybean plants comprising event soybean WO2012/08254 H2 TA SYHT0H2, methods of detecting and using 8A2

PARTICIP the same, and soybean plants comprising a

ATIONS heterologous insert at the same site as

AG SYHT0H2. MON8 MONSAN The invention provides cotton event MON cotton WO2012/13480 8701 TO 88701, and plants, plant cells, seeds, plant 8A1

TECHNO parts, and commodity products comprising

LOGY event MON 88701. The invention also

LLC provides polynucleotides specific for event

MON 88701 and plants, plant cells, seeds,

plant parts, and commodity products

comprising polynucleotides specific for

event MON 88701. The invention also

provides methods related to event MON

88701.

KK179- MONSAN The present invention provides a transgenic alfalfa WO201300355 2 TO alfalfa event KK179-2. The invention also 8A1

TECHNO provides cells, plant parts, seeds, plants,

LOGY commodity products related to the event,

LLC ; and DNA molecules that are unique to the

FORAGE event and were created by the insertion of

GENETIC transgenic DNA into the genome of a alfalfa

S plant. The invention further provides

INTERNA methods for detecting the presence of said

TIONAL alfalfa event nucleotide sequences in a

LLC sample, probes and primers for use in

detecting nucleotide sequences that are

diagnostic for the presence of said alfalfa

event.

pDAB8 DOW This invention relates to soybean event soybean WO201301009 264.42. AGROSCI pDAB8264.42.32.1 and includes novel 4A1

32.1 ENCES expression cassettes and transgenic inserts

LLC ; MS comprising multiple traits conferring

TECHNO resistance to glyphosate, aryloxyalkanoate,

LOGIES and glufosinate herbicides. This invention

LLC also relates in part to methods of controlling

resistant weeds, plant breeding and herbicide

tolerant plants. In some embodiments, the

event sequence can be "stacked" with other

traits, including, for example, other

herbicide tolerance gene(s) and/or insect- inhibitory proteins. This invention further

relates in part to endpoint TAQMAN PCR

assays for the detection of Event

pDAB8264.42.32.1 in soybeans and related

plant material. Some embodiments can

perform high throughput zygosity analysis

of plant material and other embodiments can

be used to uniquely identify the zygosity of

and breed soybean lines comprising the

event of the subject invention. Kits and

conditions useful in conducting these assays

are also provided. MZDT SYNGNE A transgenic corn event designated maize WO201301277 09Y TA MZDT09Y is disclosed. The invention 5A1

PARTICIP relates to nucleic acids that are unique to

ATIONS event MZDT09Y and to methods of

AG detecting the presence of event MZDT09Y

based on DNA sequences of the recombinant

constructs inserted into the corn genome that

resulted in the MZDT09Y event and of

genomic sequences flanking the insertion

site. The invention further relates to corn

plants comprising the transgenic genotype of

event MZDT09Y and to methods for

producing a corn plant by cross-' ing a corn

plant comprising the MZDT09Y genotype

with itself or another corn variety. Seeds of

corn plants comprising the MZDT09Y

genotype are also objects of the invention.

[0046] Plants that may be treated according to the invention are hybrid plants that already express the characteristic of heterosis or hybrid vigor which results in generally higher yield, vigor, health and resistance towards biotic and abiotic stresses). Such plants are typically made by crossing an inbred male- sterile parent line (the female parent) with another inbred male-fertile parent line (the male parent).

Hybrid seed is typically harvested from the male sterile plants and sold to growers. Male sterile plants can sometimes (e.g. in corn) be produced by detasseling, i.e. the mechanical removal of the male reproductive organs (or males flowers) but, more typically, male sterility is the result of genetic determinants in the plant genome. In that case, and especially when seed is the desired product to be harvested from the hybrid plants it is typically useful to ensure that male fertility in the hybrid plants is fully restored. This can be accomplished by ensuring that the male parents have appropriate fertility restorer genes which are capable of restoring the male fertility in hybrid plants that contain the genetic determinants responsible for male-sterility. Genetic determinants for male sterility may be located in the cytoplasm. Examples of cytoplasmic male sterility (CMS) were for instance described in Brassica species (WO 92/05251, WO 95/09910, WO 98/27806, WO 05/002324, WO 06/021972 and US 6,229,072). However, genetic determinants for male sterility can also be located in the nuclear genome. Male sterile plants can also be obtained by plant biotechnology methods such as genetic engineering. A particularly useful means of obtaining male-sterile plants is described in WO 89/10396 in which, for example, a ribonuclease such as barnase is selectively expressed in the tapetum cells in the stamens. Fertility can then be restored by expression in the tapetum cells of a ribonuclease inhibitor such as barstar (e.g. WO 91/02069).

[0047] Plants or plant cultivars (obtained by plant biotechnology methods such as genetic engineering) which may be treated according to the invention are herbicide-tolerant plants, i.e. plants made tolerant to one or more given herbicides. Such plants can be obtained either by genetic transformation, or by selection of plants containing a mutation imparting such herbicide tolerance. _{Λ Λ}

- 44 -

[0048] Herbicide-resistant plants are for example glyphosate-tolerant plants, i.e. plants made tolerant to the herbicide glyphosate or salts thereof. Plants can be made tolerant to glyphosate through different means. For example, glyphosate-tolerant plants can be obtained by transforming the plant with a gene encoding the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Examples of such EPSPS genes are the AroA gene (mutant CT7) of the bacterium Salmonella typhimurium {Science 1983, 221, 370-371), the CP4 gene of the bacterium Agrobacterium sp. (Curr. Topics Plant Physiol. 1992, 7, 139- 145), the genes encoding a Petunia EPSPS (Science 1986, 233, 478-481), a Tomato EPSPS (J. Biol. Chem. 1988, 263, 4280-4289), or an Eleusine EPSPS (WO 01/66704). It can also be a mutated EPSPS as described in for example EP 0837944, WO 00/66746, WO 00/66747 or WO 02/26995, WO 11/000498. Glyphosate-tolerant plants can also be obtained by expressing a gene that encodes a glyphosate oxido- reductase enzyme as described in US 5,776,760 and US 5,463,175. Glyphosate-tolerant plants can also be obtained by expressing a gene that encodes a glyphosate acetyl transferase enzyme as described in for example WO 02/036782, WO 03/092360, WO 05/012515 and WO 07/024782. Glyphosate-tolerant plants can also be obtained by selecting plants containing naturally- occurring mutations of the above-mentioned genes, as described in for example WO 01/024615 or WO 03/013226. Plants expressing EPSPS genes that confer glyphosate tolerance are described in e.g. U.S. Patent Applications 11/517,991, 10/739,610, 12/139,408, 12/352,532, 11/312,866, 11/315,678, 12/421,292, 11/400,598, 11/651,752, 11/681,285, 11/605,824, 12/468,205, 11/760,570, 11/762,526, 11/769,327, 11/769,255, 11/943801 or 12/362,774. Plants comprising other genes that confer glyphosate tolerance, such as decarboxylase genes, are described in e.g. U.S. Patent Applications 11/588,811, 11/185,342, 12/364,724, 11/185,560 or

12/423,926.

[0049] Other herbicide resistant plants are for example plants that are made tolerant to herbicides inhibiting the enzyme glutamine synthase, such as bialaphos, phosphinothricin or glufosinate. Such plants can be obtained by expressing an enzyme detoxifying the herbicide or a mutant glutamine synthase enzyme that is resistant to inhibition, e.g. described in U.S. Patent Application 11/760,602. One such efficient detoxifying enzyme is an enzyme encoding a phosphinothricin acetyltransferase (such as the bar or pat protein from Streptomyces species). Plants expressing an exogenous phosphinothricin

acetyltransferase are for example described in U.S. Patents 5,561,236; 5,648,477; 5,646,024; 5,273,894; 5,637,489; 5,276,268; 5,739,082; 5,908,810 and 7,112,665. [0050] Further herbicide-tolerant plants are also plants that are made tolerant to the herbicides inhibiting the enzyme hydroxyphenylpyruvatedioxygenase (HPPD). HPPD is an enzyme that catalyze the reaction in which para-hydroxyphenylpyruvate (HPP) is transformed into homogentisate. Plants tolerant to HPPD- inhibitors can be transformed with a gene encoding a naturally- occurring resistant HPPD enzyme, or a gene encoding a mutated or chimeric HPPD enzyme as described in WO 96/38567, WO 99/24585, WO 99/24586, WO 09/144079, WO 02/046387, US 6,768,044, WO 11/076877, WO 11/076882, WO 11/076885, WO 11/076889. WO 11/076892. WO13/026740, WO13/092552, WO13/092551 or _Λ

- 45 -

WO12/092555. Tolerance to HPPD-inhibitors can also be obtained by transforming plants with genes encoding certain enzymes enabling the formation of homogentisate despite the inhibition of the native HPPD enzyme by the HPPD-inhibitor. Such plants and genes are described in WO 99/34008 and WO 02/36787. Tolerance of plants to HPPD inhibitors can also be improved by transforming plants with a gene encoding an enzyme having prephenate deshydrogenase (PDH) activity in addition to a gene encoding an HPPD-tolerant enzyme, as described in WO 04/024928. Further, plants can be made more tolerant to HPPD-inhibitor herbicides by adding into their genome a gene encoding an enzyme capable of metabolizing or degrading HPPD inhibitors, such as the CYP450 enzymes shown in WO 07/103567 and WO 08/150473. [0051 ] Still further herbicide resistant plants are plants that are made tolerant to acetolactate synthase (ALS) inhibitors. Known ALS-inhibitors include, for example, sulfonylurea, imidazolinone, triazolo- pyrimidines, pyrimidinyoxy(thio)benzoates, and/or sulfonylaminocarbonyltriazolinone herbicides.

Different mutations in the ALS enzyme (also known as acetohydroxyacid synthase, AHAS) are known to confer tolerance to different herbicides and groups of herbicides, as described for example in Tranel and Wright (Weed Science 2002, 50, 700-712), but also, in U.S. Patents 5,605,011, 5,378,824, 5,141,870, and 5,013,659. The production of sulfonylurea-tolerant plants and imidazolinone-tolerant plants is described in U.S. Patents 5,605,011 ; 5,013,659; 5,141,870; 5,767,361 ; 5,731,180; 5,304,732; 4,761,373; 5,331,107; 5,928,937; and 5,378,824; and WO 96/33270. Other imidazolinone-tolerant plants are also described in for example WO 04/040012, WO 04/106529, WO 05/020673, WO 05/093093, WO 06/007373, WO 06/015376, WO 06/024351, and WO 06/060634. Further sulfonylurea- and imidazolinone-tolerant plants are also described in for example WO 07/024782, WO 2011/076345, WO 2012058223, WO 2012150335 and U.S. Patent Application 61/288958.

[0052] Other plants tolerant to imidazolinone and/or sulfonylurea can be obtained by induced mutagenesis, selection in cell cultures in the presence of the herbicide or mutation breeding as described for example for soybeans in US 5,084,082, for rice in WO 97/41218, for sugar beet in US 5,773,702 and WO 99/057965, for lettuce in US 5,198,599, or for sunflower in WO 01/065922.

[0053] Plants tolerant to 2,4 D or dicamba are for example described in US6153401.

[0054] Plants or plant cultivars (obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are insect-resistant transgenic plants, i.e. plants made resistant to attack by certain target insects. Such plants can be obtained by genetic transformation, or by selection of plants containing a mutation imparting such insect resistance.

[0055] An "insect-resistant transgenic plant", as used herein, includes any plant containing at least one transgene comprising a coding sequence encoding: 1) an insecticidal crystal protein from Bacillus thuringiensis or an insecticidal portion thereof, such as the insecticidal crystal proteins listed by Crickmore et al. (Microbiology and Molecular Biology Reviews 1998, 62, 807-813), updated by Crickmore et al. (2005) at the Bacillus thuringiensis toxin nomenclature, online at: http://www.lifesci.sussex.ac.ul^Home/Neil_Crickmore/Bt/), or insecticidal portions thereof, e.g., proteins of the Cry protein classes CrylAb, CrylAc, CrylB, CrylC, CrylD,

CrylF, Cry2Ab, Cry3Aa, or Cry3Bb or insecticidal portions thereof (e.g. EP-A 1 999 141 and WO 07/107302), or such proteins encoded by synthetic genes as e.g. described in and U.S. Patent Application 12/249,016 ; or

2) a crystal protein from Bacillus thuringiensis or a portion thereof which is insecticidal in the presence of a second other crystal protein from Bacillus thuringiensis or a portion thereof, such as the binary toxin made up of the Cry34 and Cry35 crystal proteins {Nat. Biotechnol. 2001, 19, 668-72; Applied Environm. Microbiol. 2006, 71, 1765-1774) or the binary toxin made up of the CrylA or CrylF proteins and the Cry2Aa or Cry2Ab or Cry2Ae proteins (U.S. Patent Application 12/214,022 and EP- A 2 300 618); or 3) a hybrid insecticidal protein comprising parts of different insecticidal crystal proteins from Bacillus thuringiensis, such as a hybrid of the proteins of 1) above or a hybrid of the proteins of 2) above, e.g., the CrylA.105 protein produced by corn event MON89034 (WO 07/027777); or

4) a protein of any one of 1) to 3) above wherein some, particularly 1 to 10, amino acids have been replaced by another amino acid to obtain a higher insecticidal activity to a target insect species, and/or to expand the range of target insect species affected, and/or because of changes introduced into the encoding DNA during cloning or transformation, such as the Cry3Bbl protein in corn events MON863 or MON88017, or the Cry3A protein in corn event MIR604; or

5) an insecticidal secreted protein from Bacillus thuringiensis or Bacillus cereus, or an insecticidal portion thereof, such as the vegetative insecticidal (VIP) proteins listed at: http://www.lifesci.sussex.ac.ul^ome/Neil_Crickniore/Bt/vip.html, e.g., proteins from the VIP3Aa protein class; or

6) a secreted protein from Bacillus thuringiensis or Bacillus cereus which is insecticidal in the presence of a second secreted protein from Bacillus thuringiensis or B. cereus, such as the binary toxin made up of the VIP1A and VIP2A proteins (WO 94/21795); or 7) a hybrid insecticidal protein comprising parts from different secreted proteins from Bacillus thuringiensis or Bacillus cereus, such as a hybrid of the proteins in 1) above or a hybrid of the proteins in 2) above; or a protein of any one of 5) to 7) above wherein some, particularly 1 to 10, amino acids have been replaced by another amino acid to obtain a higher insecticidal activity to a target insect species, and/or to expand the range of target insect species affected, and/or because of changes introduced into the encoding DNA during cloning or transformation (while still encoding an insecticidal protein), such as the VIP3Aa protein in cotton event COT102; or a secreted protein from Bacillus thuringiensis or Bacillus cereus which is insecticidal in the presence of a crystal protein from Bacillus thuringiensis, such as the binary toxin made up of VIP3 and Cryl A or CrylF (U.S. Patent Applications 61/126083 and 61/195019), or the binary toxin made up of the VIP3 protein and the Cry2Aa or Cry2Ab or Cry2Ae proteins (U.S. Patent Application 12/214,022 and EP-A 2 300 618).

10) a protein of 9) above wherein some, particularly 1 to 10, amino acids have been replaced by another amino acid to obtain a higher insecticidal activity to a target insect species, and/or to expand the range of target insect species affected, and/or because of changes introduced into the encoding DNA during cloning or transformation (while still encoding an insecticidal protein)

[0056] Of course, an insect-resistant transgenic plant, as used herein, also includes any plant comprising a combination of genes encoding the proteins of any one of the above classes 1 to 10. In one embodiment, an insect-resistant plant contains more than one transgene encoding a protein of any one of the above classes 1 to 10, to expand the range of target insect species affected when using different proteins directed at different target insect species, or to delay insect resistance development to the plants by using different proteins insecticidal to the same target insect species but having a different mode of action, such as binding to different receptor binding sites in the insect.

[0057] An "insect-resistant transgenic plant", as used herein, further includes any plant containing at least one transgene comprising a sequence producing upon expression a double-stranded RNA which upon ingestion by a plant insect pest inhibits the growth of this insect pest, as described e.g. in WO 07/080126, WO 06/129204, WO 07/074405, WO 07/080127 and WO 07/035650.

[0058] Plants or plant cultivars (obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are tolerant to abiotic stresses. Such plants can be obtained by genetic transformation, or by selection of plants containing a mutation imparting such stress resistance. Particularly useful stress tolerance plants include: plants which contain a transgene capable of reducing the expression and/or the activity of poly(ADP- ribose) polymerase (PARP) gene in the plant cells or plants as described in WO 00/04173, WO 06/045633, EP-A 1 807 519, or EP-A 2 018 431. _

- 48 -

2) plants which contain a stress tolerance enhancing transgene capable of reducing the expression and/or the activity of the PARG encoding genes of the plants or plants cells, as described e.g. in WO 04/090140.

3) plants which contain a stress tolerance enhancing transgene coding for a plant-functional enzyme of the nicotineamide adenine dinucleotide salvage synthesis pathway including nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase, nicotinamide adenine dinucleotide synthetase or nicotine amide phosphorybosyltransferase as described e.g. in EP- A 1 794 306, WO 06/133827, WO 07/107326, EP-A 1 999 263, or WO 07/107326.

[0059] Plants or plant cultivars (obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention show altered quantity, quality and/or storage- stability of the harvested product and/or altered properties of specific ingredients of the harvested product such as:

1) transgenic plants which synthesize a modified starch, which in its physical-chemical characteristics, in particular the amylose content or the amylose/amylopectin ratio, the degree of branching, the average chain length, the side chain distribution, the viscosity behaviour, the gelling strength, the starch grain size and/or the starch grain morphology, is changed in comparison with the synthesised starch in wild type plant cells or plants, so that this is better suited for special applications. Said transgenic plants synthesizing a modified starch are disclosed, for example, in EP-A 0 571 427, WO 95/04826, EP-A 0 719 338, WO 96/15248, WO 96/19581, WO 96/27674, WO 97/11188, WO 97/26362, WO 97/32985, WO 97/42328, WO 97/44472, WO 97/45545, WO 98/27212, WO

98/40503, WO 99/58688, WO 99/58690, WO 99/58654, WO 00/08184, WO 00/08185, WO 00/08175, WO 00/28052, WO 00/77229, WO 01/12782, WO 01/12826, WO 02/101059, WO 03/071860, WO 04/056999, WO 05/030942, WO 05/030941, WO 05/095632, WO 05/095617, WO 05/095619, WO 2005/095618, WO 05/123927, WO 06/018319, WO 06/103107, WO 06/108702, WO 07/009823, WO 00/22140, WO 06/063862, WO 06/072603, WO 02/034923, WO 08/017518,

WO 08/080630, WO 08/080631, WO 08/090008, WO 01/14569, WO 02/79410, WO 03/33540, WO 04/078983, WO 01/19975, WO 95/26407, WO 96/34968, WO 98/20145, WO 99/12950, WO 99/66050, WO 99/53072, US 6,734,341, WO 00/11192, WO 98/22604, WO 98/32326, WO 01/98509, WO 01/98509, WO 05/002359, US 5,824,790, US 6,013,861, WO 94/04693, WO 94/09144, WO 94/11520, WO 95/35026, WO 97/20936, WO 10/012796, WO 10/003701, WO

13/053729, WO 13/053730,

2) transgenic plants which synthesize non starch carbohydrate polymers or which synthesize non starch carbohydrate polymers with altered properties in comparison to wild type plants without genetic modification. Examples are plants producing polyfructose, especially of the inulin and levan-type, as disclosed in EP-A 0 663 956, WO 96/01904, WO 96/21023, WO 98/39460, and WO 99/24593, _

- 49 - plants producing alpha- 1,4-glucans as disclosed in WO 95/31553, US 2002031826, US 6,284,479, US 5,712,107, WO 97/47806, WO 97/47807, WO 97/47808 and WO 00/14249, plants producing alpha-1,6 branched alpha- 1,4-glucans, as disclosed in WO 00/73422, plants producing alternan, as disclosed in e.g. WO 00/47727, WO 00/73422, US 5,908,975 and EP-A 0 728 213, 3) transgenic plants which produce hyaluronan, as for example disclosed in WO 06/032538, WO 07/039314, WO 07/039315, WO 07/039316, JP-A 2006-304779, and WO 05/012529.

4) transgenic plants or hybrid plants, such as onions with characteristics such as 'high soluble solids content', 'low pungency' (LP) and/or 'long storage' (LS), as described in U.S. Patent Applications 12/020,360. 5) Transgenic plants displaying an increase yield as for example disclosed in WO 11/095528

[0060] Plants or plant cultivars (that can be obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are plants, such as cotton plants, with altered fiber characteristics. Such plants can be obtained by genetic transformation, or by selection of plants contain a mutation imparting such altered fiber characteristics and include: a) Plants, such as cotton plants, containing an altered form of cellulose synthase genes as described in WO 98/00549. b) Plants, such as cotton plants, containing an altered form of rsw2 or rsw3 homologous nucleic acids as described in WO 04/053219. c) Plants, such as cotton plants, with increased expression of sucrose phosphate synthase as described in WO 01/17333. d) Plants, such as cotton plants, with increased expression of sucrose synthase as described in WO 02/45485. e) Plants, such as cotton plants, wherein the timing of the plasmodesmatal gating at the basis of the fiber cell is altered, e.g. through downregulation of fiber-selective -l,3-glucanase as described in WO

05/017157, or as described in WO 09/143995. f) Plants, such as cotton plants, having fibers with altered reactivity, e.g. through the expression of N- acetylglucosaminetransferase gene including nodC and chitin synthase genes as described in WO 06/136351, WO 11/089021, WO 11/089021, WO 12/074868. _5Q

[0061] Plants or plant cultivars (that can be obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are plants, such as oilseed rape or related Brassica plants, with altered oil profile characteristics. Such plants can be obtained by genetic transformation, or by selection of plants contain a mutation imparting such altered oil profile characteristics and include: a) Plants, such as oilseed rape plants, producing oil having a high oleic acid content as described e.g. in US 5,969,169, US 5,840,946 or US 6,323,392 or US 6,063,947 b) Plants such as oilseed rape plants, producing oil having a low linolenic acid content as described in US 6,270,828, US 6,169,190, US 5,965,755 or WO 11/060946 c) Plant such as oilseed rape plants, producing oil having a low level of saturated fatty acids as described e.g. in US 5,434,283 or U.S. Patent Application 12/668303 d) Plants such as oilseed rape plants, producing oil having an alter glucosinolate content as described in WO 2012075426.

[0062] Plants or plant cultivars (that can be obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are plants, such as oilseed rape or related Brassica plants, with altered seed shattering characteristics. Such plants can be obtained by genetic transformation, or by selection of plants contain a mutation imparting such altered seed shattering characteristics and include plants such as oilseed rape plants with delayed or reduced seed shattering as described in WO 2009/068313 and WO 2010/006732, WO 2012090499. [0063] Plants or plant cultivars (that can be obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are plants, such as Tobacco plants, with altered post-translational protein modification patterns, for example as described in WO 10/121818 and WO 10/145846.

[0064] Particularly useful transgenic plants which may be treated according to the invention are plants containing transformation events, or combination of transformation events, that are the subject of petitions for non-regulated status, in the United States of America, to the Animal and Plant Health Inspection Service (APHIS) of the United States Department of Agriculture (USDA) whether such petitions are granted or are still pending. At any time this information is readily available from APHIS (4700 River Road, Riverdale, MD 20737, USA), for instance on its internet site (URL

http://www.aphis.usda.gov/brs/not_reg.html). On the filing date of this application the petitions for nonregulated status that were pending with APHIS or granted by APHIS were those listed in Table B which contains the following information: Petition: the identification number of the petition. Technical descriptions of the transformation events can be found in the individual petition documents which are obtainable from APHIS, for example on the APHIS website, by reference to this petition number. These descriptions are herein incorporated by reference.

Extension of Petition: reference to a previous petition for which an extension is requested. Institution: the name of the entity submitting the petition. Regulated article: the plant species concerned.

Transgenic phenotype: the trait conferred to the plants by the transformation event.

Transformation event or line: the name of the event or events (sometimes also designated as lines or lines) for which nonregulated status is requested.

APHIS documents: various documents published by APHIS in relation to the Petition and which can be requested with APHIS.

Table B

Petition No. Applicant Crop Phenotype/Event

11-342-Olp Genective Corn Glyphosate Tolerant/ VCO-01981-5

11-234-Olp Dow Soybean 2, 4-D, Glyphosate and Glufosinate Tolerant/ DAS- 44406-6

11-202-Olp Monsanto Soybean Increased Yield/ MON 87712

11-188-Olp Monsanto Canola Glyphosate Tolerant/ MON 88302

11-063-Olp Pioneer Canola Glyphosate Tolerant/73496

10-281-Olp Monsanto Corn Male Sterile/ MON 87427

10-188-Olp Monsanto Soybean Dicamba Tolerant/ MON 87708

10-161-Olp Okanagan Apple Non-Browning/ GD743, GS784

09-015-01p BASF Soybean Imadazolinone Tolerant/ BPS-CV127-9

The following pending petitions will proceed with the previous process for soliciting public input (simultaneous notice of availability of the petition and decisionmaking documents). Petition No. Applicant Crop Phenotype/Event

12-033-01p Bayer Cotton Glufosinate Tolerant, Lepidopteran Resistant/

Extension of T303-3

08-340-01p

11-244-Olp Pioneer Corn Insect Resistant and Glufosinate Tolerant/ DP-004114-3

10-336-Olp Syngenta Corn Rootworm Resistant/

5307

09-349-01p Dow Soybean 2,4-D and Glufosinate Tolerant/ DAS-68416-4

09-328-01p Bayer Soybean Glyphosate and Isoxaflutole Tolerant/ FG72

09-233-01p Dow Corn 2,4-D and ACCase-Inhibitor Tolerant/ DAS-40278-9

03-104-01p Scotts Creeping Glyphosate Tolerant/

Bentgrass ASR368

Determinations of Nonregulated

Status

Petition No. Applicant Crop Phenotype/Event

09-201-01p Monsanto Soybean Improved Fatty Acid Profile/

MON 87705

09-183-01p Monsanto Soybean Stearidonic Acid Produced/

MON 87769

09-082-01p Monsanto Soybean Insect Resistant/

MON 87701

09-055-01p Monsanto Corn Drought Tolerant/

MON 87460

08-340-01p Bayer Cotton Glufosinate Tolerant, Lepidopteran Resistant/ T304-40 x GHB119 08-338-01p Pioneer Corn Male Sterile, Fertility Restored, Visual

Marker/

DP-32138-1

08-315-01p Florigene Rose Altered Flower Color/

IFD-52401-4,

IFD-52901-9

07-253-01p Syngenta Corn Lepidopteran Resistant/

MIR 162

07-152-01p Pioneer Corn Glyphosate & Imidazolinone Tolerant/ 98140

07-108-01p Syngenta Cotton Lepidopteran Resistant/

COT67B

06-354-01p Pioneer Soybean High Oleic Acid/

Event 305423

06-332-01p Bayer Crop Science Cotton Glyphosate Tolerant/

GHB614

06-298-01p Monsanto Corn European Corn Borer Resistant/ MON 89034

06-271-01p Pioneer Soybean Glyphosate & Acetolactate Synthase Tolerant/ DP-356043-5

06-234-01p Bayer Crop Science Rice Phosphinothricin Tolerant/

LLRICE601

Extension of

98-329-01p

06-178-01p Monsanto Soybean Glyphosate Tolerant/

MON 89788

05-280-01p Syngenta Corn Thermostable Alpha-amylase/ 3272

04-362-01p Syngenta Corn Corn Rootworm Protected/

MIR604

04-337-01p University of Papaya Papaya Ringspot Virus Resistant/

Florida

XI 7-2

04-264-01p ARS Plum Plum Pox Virus Resistant/

C5

04-229-01p Monsanto Corn High Lysine/

LY038

04-125-01p Monsanto Corn Corn Rootworm Resistant/ MON 88017

04-110- Monsanto & Forage Alfalfa Glyphosate Tolerant/

Olp al Genetics

J101, J103

04-110-Olp

04-086-01p Monsanto Cotton Glyphosate Tolerant/

MON 88913

03-353-01p Dow Corn Corn Rootworm Resistant/

59122

03-323- Monsanto and Sugar Beet Glyphosate Tolerant/

Olp al KWS SAAT AG

H7-1

03-323-01p

03-181-01p Dow Corn Lepidopteran Resistant & Phosphinothricin

Tolerant/

6275

Extension of

00-136-Olp

03-155-01p Syngenta Cotton Lepidopteran Resistant/

COT102

03-036-02p Mycogen/Dow Cotton Lepidopteran Resistant/

3006-210-23

03-036-01p Mycogen/Dow Cotton Lepidopteran Resistant/

281-24-236

02-042-01p Aventis Cotton Phosphinothericin Tolerant/

LLCotton25

01-324-Olp Monsanto Rapeseed Glyphosate tolerant/

GT200

Extension of

98-216-01p

01-206-02p Aventis Rapeseed Phosphinothricin Tolerant & Pollination

Control/

Topas 19/2

Extension of

97-205-01p

01-206-Olp Aventis Rapeseed Phosphinothricin Tolerant/

MSI

Extension of

98-278-01p

01-137-Olp Monsanto Corn Corn Rootworm Resistant/

MON 863

01-121-Olp Vector Tobacco Reduced Nicotine/

Vector 21-41 00-342-Olp Monsanto Cotton Lepidopteran Resistant/

15985

00-136-Olp Mycogen c/o Dow Corn Lepidopteran Resistant Phosphinothricin

& Pioneer Tolerant/

1507

00-011-Olp Monsanto Corn Glyphosate Tolerant/

NK603

Extension of

97-099-01p

99-173-01p Monsanto Potato Potato Leafroll Virus & Colorado Potato

Beetle Resistant/

RBMT22-82

Extension of

97-204-01p

98-349-01p AgrEvo Corn Phosphinothricin Tolerant and Male Sterile/ MS6

Extension of

95-228-01p

98-335-01p U. of Saskatchewan Flax Tolerant to Soil Residues of Sulfonylurea

Herbicide/

CDC Triffid

98-329-01p AgrEvo Rice Phosphinothricin Tolerant/

LLRICE06, LLRICE62

98-278-01p AgrEvo Rapeseed Phosphinothricin Tolerant and Pollination

Control/

MS8, RF3

98-238-01p AgrEvo Soybean Phosphinothricin Tolerant/

GU262

98-216-01p Monsanto Rapeseed Glyphosate Tolerant/

RT73

98-173-01p Novartis Seeds & Beet Glyphosate Tolerant/

Monsanto GTSB77

98-014-01p AgrEvo Soybean Phosphinothricin Tolerant/

A5547-127

Extension of

96-068-01p

97-342-01p Pioneer Corn Male Sterile and Phosphinothricin Tolerant/ 676, 678, 680 _r

- 56 -

97-339-01p Monsanto Potato Colorado Potato Beetle and Potato Virus Y

Resistant/

RBMT15-101, SEMT15-02, SEMT15-15

97-336-01p AgrEvo Beet Phosphinothricin Tolerant/

T120-7

97-287-01p Monsanto Tomato Lepidopteran Resistant/

5345

97-265-01p AgrEvo Corn Phosphinothricin Tolerant and Lepidopteran

Resistant/

CBH-351

97-205-01p AgrEvo Rapeseed Phosphinothricin Tolerant/

T45

97-204-01p Monsanto Potato Potato Leafroll Virus & Colorado Potato

Beetle Resistant/

RBMT21-129, RBMT21-152, RBMT21-350, RBMT22-82, RBMT22-186, RBMT22-238,

RBMT22-262

97-148-01p Bejo Cichorium Male Sterile/

intybus RM3-3, RM3-4, RM3-6

97-099-01p Monsanto Corn Glyphosate Tolerant/

GA21

97-013-01p Calgene Cotton Bromoxynil Tolerant and Lepidopteran

Resistant/

31807, 31808

97-008-01p Du Pont Soybean High Oleic Acid Oil/

G94-1, G94-19, G-168

96-317-01p Monsanto Corn Glyphosate Tolerant and European Corn Borer

Resistant/

MON 802

96-291-01p DeKalb Corn European Corn Borer Resistant/ DBT418

96-248-01p Calgene Tomato Fruit Ripening Altered/

532A 4109a 5166

Extension of

92-196-01p

96-068-01p AgrEvo Soybean Glufosinate Tolerant/ „

W62, W98, A2704-12, A2704-21, A5547-35

96-051-01p Cornell U Papaya Papaya Ringspot Virus Resistant/ 55-1, 63-1

96-017-01p Monsanto Corn European Corn Borer Resistant/ MON 809, MON 810

Extension of

95-093-01p

95-352-01p As grow Squash Cucumber Mosaic Virus, Watermelon Mosaic

Virus 2, and Zucchini Yellow Mosaic Virus

Resistant/

CZW-3

95-338-01p Monsanto Potato Colorado Potato Beetle Resistant/

SPBT02-5, SPBT02-7, ATBT04-6, ATBT04- 27, ATBT04-30, ATBT04-31, ATBT04-36

95-324-01p Agritope Tomato Fruit Ripening Altered/

35-1-N

95-256-01p Du Pont Cotton Sulfonylurea Tolerant/

19-51A

95-228-01p Plant Genetic Corn Male Sterile/MS3

Systems

95-195-01p Northrup King Corn European Corn Borer Resistant/ Btl l

95-179-01p Calgene Tomato Fruit Ripening Altered/

519a 4109a-4645,

Extension of 540a 4109a-1823

92-196-01p

95-145-01p DeKalb Corn Glufosinate Tolerant/

B16

95-093-01p Monsanto Corn Lepidopteran Resistant/

MON 80100

95-053-01p Monsanto Tomato Fruit Ripening Altered/

8338

95-045-01p Monsanto Cotton Glyphosate Tolerant/ 1445, 1698

95-030-01p Calgene Tomato Fruit Ripening Altered/

105F 1436 2018, 105F 1436 2035, 105F 1436 2049, 35F 4109a 3023, 84F 4109a 148, 88F 4109a 2797, 121F 4109a 333, 121F 4109a 1071, 121F 4109a 1120, 137F 4109a 71, 138F 4109a 164, 519A 4109a 4527, 519A 4109a 4621, 519A 4109a 4676, 531A 4109a 2105, 531A 4109a 2270, 532A 4109a 5097, 540A 4109a 1739, 585A 4109a 3604, 585A 4109a

3530

Extension of

92-196-01p

94-357-01p AgrEvo Corn Glufosinate Tolerant/

T14, T25

94-319-01p Ciba Seeds Corn Lepidopteran Resistant/

176

94-308-01p Monsanto Cotton Lepidopteran Resistant/

531, 757, 1076

94-290-01p Zeneca & Petoseed Tomato Fruit Polygalacturonase Level Decreased/ B, Da, F

94-257-01p Monsanto Potato Coleopteran Resistant/

BT6, BT10, BT12, BT16, BT17, BT18, BT23

94-230-01p Calgene Tomato Fruit Ripening Altered/

114F 4109a 26,

Extension of 114F 4109a 81

92-196-01p

94-228-01p DNA Plant Tech Tomato Fruit Ripening Altered/

1345-4

94-227-01p Calgene Tomato Fruit Ripening Altered/

pCGN1436, pCGN4109

Extension of

92-196-01p

94-090-01p Calgene Rapeseed Oil Profile Altered/

pCGN3828-212/86- 18, pCGN3828-212/86-23

93-258-01p Monsanto Soybean Glyphosate Tolerant/

4-30-2

93-196-01p Calgene Cotton Bromoxynil Tolerant/

BXN

92-204-01p Upjohn Squash Watermelon Mosaic Virus and Zucchini

Yellow Mosaic Virus Resistant/

ZW-20

92-196-01p Calgene Tomato Fruit Ripening Altered/

pCGN1547, pCGN1548, pCGN1557, pCGN1559, pCGN1578

[0065] Additional particularly useful plants containing single transformation events or combinations of transformation events are listed for example in the databases from various national or regional regulatory agencies (see for example http://gmoinfo.jrc.it/gmp_browse.aspx and http://www.cera- gmc.org/?action=gm_crop_database ).

[0066] Further particularly transgenic plants include plants containing a transgene in an agronomically neutral or beneficial position as described in any of the patent publications listed in Table C.

Table C

Trait Reference Remarks

WO 2000/073475

WO2009/150541

Water use efficiency WO2009/150541

WO2012075429

WO2012077020

WO2012158594

Nitrogen use efficiency WO 1995/009911

WO 1997/030163

WO 2007/092704

WO 2007/076115

WO 2005/103270

WO 2002/002776

WO2008/051608

WO2008/112613

WO2009/015096

WO2009/061776 WO2009/105612

WO2009/117853

WO2010/006010

WO2009/117853

WO2009/061776

WO2009/015096

WO2009/105492

WO2009/105612

WO 2010/053621

WO 2010/053867

WO2010/077890

WO 2010/086220

WO 2010/111568

WO 2010/140388

WO2010/007496

WO2011/022597

WO2011/022608

WO2012087140

Improved photosynthesis WO 2008/056915

WO 2004/101751

Nematode resistance WO 1995/020669

WO 2001/051627

WO 2008/139334

WO 2008/095972

WO 2006/085966

WO 2003/033651

WO 1999/060141

WO 1998/012335

WO 1996/030517

WO 1993/018170

WO2008/095886

WO2008/095887

WO2008/095888

WO2008/095889

WO2008/095910

WO2008/095911

WO2008/095916

WO2008/095919

WO2008/095969

WO2008/095970 WO2008/110522

WO2008/139334

WO2008/152008 -

W02010/077858

WO 2010/091230 -

WO 2010/102172 -

WO 2010/106163

WO2011/003783

WO2011/082217

WO2011/104153

WO2012007916

WO2012007919

WO2012009551

WO2012011034

WO2012012403

WO2012153274

WO2012156902

WO 2006/009649

Reduced pod dehiscence WO 2004/113542

WO 1999/015680

WO 1999/000502

WO 1997/013865

WO 1996/030529

WO 1994/023043

WO 2006/125065

Aphid resistance

WO 1997/046080

WO 2008/067043

WO 2004/072109

WO2009/091860

WO2010036764

WO 2006/135717

Sclerotinia resistance

WO 2006/055851

WO 2005/090578

WO 2005/000007

WO 2002/099385

WO 2002/061043

Botrytis resistance WO 2006/046861

WO 2002/085105

Bremia resistance US 20070022496

WO 2000/063432 WO2009/111627

WO2009/111627

Erwinia resistance WO 2004/049786

Closterovirus resistance WO 2007/073167

WO 2007/053015

WO 2002/022836

Stress tolerance (including

WO 2010/019838 drought tolerance)

WO 2009/049110

WO2008/002480

WO2005/033318

WO2008/002480

WO2008/005210

WO2008/006033

WO2008/008779

WO2008/022486

WO2008/025097

WO2008/027534

WO2008/027540

WO2008/037902

WO2008/046069

WO2008/053487

WO2008/057642

WO2008/061240

WO2008/064222

WO2008/064341

WO2008/073617

WO2008/074025

WO2008/076844

WO2008/096138

WO2008/110848

WO2008/116829

WO2008/117537

WO2008/121320

WO2008/125245

WO2008/142034

WO2008/142036

WO2008/150165

WO2008/092935

WO2008/145675 WO2009/016240

WO2009/031664

WO2009/038581

WO2009/049110

WO2009/053511

WO2009/054735

WO2009/067580

WO2009/073605

WO2009/077611

WO2009/079508 Also yield

WO2009/079529

WO2009/083958

Also yield

WO2009/086229 Also yield

WO2009/092009

WO2009/094401

WO2009/094527

WO2009/102965 Also biomass/starch/oil

WO2009/114733

WO2009/117448

WO2009/126359

WO2009/126462 Also grain yield

WO2009/129162

WO2009/132057

WO2009/141824

WO2009/148330

WO 2010/055024

WO 2010/058428

WO 2010/064934

WO2010/076756

WO 2010/083178

WO 2010/086221

WO 2010/086277

WO 2010/101818

WO 2010/104848

WO 2010/118338

WO 2010/120017

WO 2010/120054

WO 2010/121316

WO 2010/127579

WO 2010/134654 WO2010/039750

WO2011/034968

WO2011/001286

WO2011/017492

WO2011/018662

WO2011/024065

WO2011/038389

WO2011/46772

WO2011/053897

WO2011/052169

WO2011/063706

WO2011/067745

WO2011/079277

WO2011/080674

WO2011/083290

WO2011/083298

WO2011/091764

WO2011/052169

WO2011/053897

WO2011/056769

WO2011/063706

WO2011/067745

WO2011/083290

WO2011/083298

WO2011/091764

WO2011/096609

WO2011/122761

WO2012176167

WO2012139532

WO2012159196

WO2012162193

WO2012167023

WO2012172556

WO2012116396

Tobamovirus resistance WO 2006/038794

WO2009086850

WO 2010/046221 NUE

WO 2010/046471

Yield

WO 2010/049897

WO 2010/055837 WO2010/069847

WO2010/075143

WO2010/075243

WO 2010/100595

WO 2010/102220 NUE WO 2010/104092

WO 2010/108836

WO 2010/120862 ABST

WO 2010/123667

WO 2010/124953

WO 2010/125036

WO 2010/127969

WO 2010/129501

WO 2010/140388

WO 2010/140672

WO2011/011273

WO2011/000466

WO2011/003800

WO2011/006717

WO2011/008510

WO2011/009801

WO2011/011412

WO2011/015985

WO2011/020746

WO2011/021190

WO2011/025514

WO2011/025515

WO2011/025516

WO2011/025840

WO2011/031680

WO2011/036160

WO2011/036232

WO2011/041796

WO2011/044254

WO2011/048009

WO2011/053898

WO2011/051120

WO2011/058029

WO2011/061656

WO2011/085062 WO2011/053898 WO2011/058029 WO2011/061656 WO2011/085062 WO2011/088065 WO2011/095958 WO2011/097215 WO2011/099006 WO2011/104128 WO2011/104141 WO2011/104143 WO2011/104155 WO2011/106734 WO2011/106794 WO2011/109661 WO2011/114279 WO2011/114305 WO2011/114312 WO2011/114313 WO2011/117800 WO2011/135527 WO2011/136909 WO2011/139431 WO2011/140329 WO2011/146754 WO2011/147826 WO2011/157976 WO2011/161617 WO2011/161620 WO2011/109618 WO2011/159452 WO2012078949 WO2012083219 WO2012084742 WO2012084756 WO2012087903 WO2012087940 WO2012090500 WO2012091939 WO2012092106 WO2012092573

WO2012092580

WO2012092596

WO2012093032

WO2012093833

WO2012097720

WO2012098517

WO2012102999

WO2012106321

WO2012158630

WO2012165678

WO2012112518

WO2012117324

WO2012117330

WO2012117368

WO2012119152

WO2012142106

WO2012142116

WO2012143830

WO2012143865

WO2012145269

WO2012148121

WO2012148122

WO2012148835

WO2012150598

WO2012153267

WO2012153277

WO2012156865

WO2012158926

Oil content/composition WO 2010/045324

WO 2010/053541

WO 2010/130725

WO 2010/140682

WO2011/006948

WO2011/049627

WO2011/060946

W02011/062748

WO2011/064181

WO2011/064183

WO2011/075716 WO2011/049627

W02011/062748

WO2011/064181

WO2011/064183

WO2011/079005

WO2011/146524

WO2011/161093

WO2011/163557

WO2011/163632

WO2011/163632

WO2012074385

WO2012074386

WO2012103452

WO2012117256

Biopharmaceutical WO 2010/121818

production

WO2011/119115

Improved recombination WO2010/071418

WO 2010/133616

plant appearance WO 2010/069004

WO2011/060552

Disease control (other) WO 2010/059558 fungi

WO2010/075352 Insects/non-Bt

WO2010/075498 insects/Bt

WO 2010/085289 insects/Bt

WO 2010/085295 insects/Bt

WO 2010/085373 insects/Bt

WO2009/000736 fungi

WO2009/065863 fungi

WO2009/112505 fungi

WO 2010/089374 bacteria

WO 2010/120452 insects/Bt

WO 2010/123904 virus

WO 2010/135782 fungi

WO2011/025860 fungi

WO2011/041256 Insects

WO2011/031006 Insects / Bt

WO2011/031922 Insects / Bt

WO2011/075584 Insects / Bt

WO2011/075585 Insects / Bt

WO2011/075586 Insects / Bt WO2011/075587 Insects / Bt

WO2011/075588 Insects / Bt

WO2011/084622 Insects / Bt

WO2011/084626 Insects / Bt

WO2011/084627 Insects / Bt

WO2011/084629 Insects / Bt

WO2011/084630 Insects / Bt

WO2011/084631 Insects / Bt

WO2011/084314 Insects / Bt

WO2011/084324 Insects / Bt

WO2011/023571 Insects / Bt

WO2011/040880

WO2011/082304

WO2011/003783

WO2011/020797

WO2011/069953 fungi

WO2011/075584 Insects/Bt

WO2011/075585 Insects/Bt

WO2011/075586 Insects/Bt

WO2011/075587 Insects/Bt

WO2011/075588 Insects/Bt

WO2011/084314 Insects/Bt

WO2011/084324 Insects/Bt

WO2011/084622 Insects/Bt

WO2011/084626 Insects/Bt

WO2011/084627 Insects/Bt

WO2011/084629 Insects/Bt

WO2011/084630 Insects/Bt

WO2011/084631 Insects/Bt

WO2011/133892 Insects/Bt

WO2011/133895 Insects/Bt

WO2011/133896 Insects/Bt

WO2011/082304

WO2011/100650

WO2011/158242

WO2012003207 Bacteria

WO2012004013 Fungi

WO2012004401 Fungi

WO2012006271 Fungi

WO2012006426 Fungi

WO2012006439 Fungi WO2012006622 General

WO2012015039

WO2012058266 Insects/ Coleoptera

WO2012058458 Insects/ Coleoptera

WO2012058528 Insects/Lepidoptera

WO2012058730 Insects/Lepidoptera

WO2012061513 Insects/Lepidoptera

WO2012063200 Insects/Lepidoptera

WO2012065166 Insects/Lepidoptera

WO2012065219 Insects/Lepidoptera

WO2012066008 Insects/non-Bt

WO2012067127 Insects/non-Bt

WO2012068966 Insects/non-Bt

WO2012071039 Insects/non-Bt

WO2012071040 Insects/non-Bt

WO2012117406 Bacteria

WO2012116938 Fungi

WO2012147635 Fungi

WO2012160528 Fungi

WO2012172498 Fung

WO2012178154 Fungi

WO2012149316 Fungi

WO2012175420 -

WO2012109515A1 Insects/ Coleoptera

WO2012109430A2 Insects and nematodes

WO2012122369A1 Insects/Lepidoptera

WO2012131619A1 Insects/Lepidoptera

WO2012139004A2 Insects/Lepidoptera

WO2012143542A1 Insects/Non-Bt

WO2012165961A1 Insects/Non-Bt

Herbicide tolerance US 4761373 imidazolinone

US 5304732 Imidazolinone

US 5331107 Imidazolinone

US 5718079 Imidazolinone

US 6211438 Imidazolinone

US 6211439 Imidazolinone

US 6222100 Imidazolinone

US 2003/0217381 Imidazolinone

US 2003/0217381 Imidazolinone

WO2004/106529 Imidazolinone WO2000/27182 Imidazolinone

WO2005/20673 imidazolinone

WO 2001/85970 Imidazolinone

US 5545822 Imidazolinone

US 5736629 Imidazolinone

US 5773703, Imidazolinone

US 5773704 Imidazolinone

US 5952553 Imidazolinone

US 6274796 Imidazolinone

WO 2004/106529 Imidazolinone

WO2004/16073 Imidazolinone

WO 2003/14357 Imidazolinone

WO 2003/13225 imidazolinone

WO 2003/14356 imidazolinone

US 5188642 glyphosate

US 4940835 glyphosate

US 5633435 glyphosate

US 5804425 glyphosate

US 5627061. glyphosate

US 5646024 glufosinate

US 5561236 glufosinate

US 6333449 glufosinate

US 6933111 glufosinate

US 6468747. glufosinate

US 6376754 glufosinate

US 7105724 dicamba

US 7105724 dicamba

WO 2008/051633 dicamba

US 7105724 dicamba

US 5670454 dicamba

US 7105724 dicamba

US 7105724 dicamba

US 7105724 dicamba

US 7105724 dicamba

US 5670454 dicamba

US 7105724 dicamba

US 7105724 dicamba

US 7105724 dicamba

US 5670454 dicamba

US 7105724 dicamba

US 7105724 dicamba US 7105724 dicamba

US 7105724 dicamba

US 6153401 2,4-D

US 6100446 2,4-D

WO 2005/107437 2,4-D

US 5670454 2,4-D

US 5608147 2,4-D

US 5670454 2,4-D

WO 2004/055191 HPPD-inhibitor

WO 199638567 HPPD-inhibitor

US 6791014 HPPD-inhibitor

US 2002/0073443, Protox-inhibitor

US 20080052798 Protox-inhibitor

WO2011/022470

WO2011/034936

WO2011/028832

WO2011/028833

WO2011/028836

WO2011/068567 HPPD-inhibitor

WO2011/076345 HPPD-inhibitor

WO2011/085221 HPPD-inhibitor

WO2011/094199

WO2011/094205 HPPD-inhibitor

WO2011/068567 HPPD-inhibitor

WO2011/085221 saflufenacil

WO2011/094199 HPPD-inhibitor

WO2011/094205 HPPD-inhibitor

WO2011/145015 HPPD-inhibitor

WO2012047595 2,4-D

WO2012048124 ACCase-inhibotor

WO2012048136 Glyphosate

WO2012048807 Glyphosate

WO2012049663 Glyphosate

WO2012050962 Glyphosate

WO2012056401 HPPD-inhibitor

WO2012057466 PPX

WO2012057465 Protox-inhibitor

WO2012058223 ALS/SU

WO2012115968 ,4-D

WO2012148818 2,4-D

WO2012148820 2,4-D WO2012124808 Dicamba

WO2012148275 Glyphosate

plant metabolism WO2011/060920

WO2011/119115

WO2011/102394

reproduction/pollination WO2011/113839

control

WO2012142311

WO2012163389

Biofuels WO2012073493

Fruit ripening WO2012073494

Fiber quality WO2012074386

WO2012115697

Carbohydrates

WO2012132348

WO2012134906

WO2012174462

[0067] Additional particularly useful plants containing single transformation events or combinations of transformation events are listed for example in the databases from various national or regional regulatory agencies (see for example http://gmoinfo.jrc.it/gmp_browse.aspx and http://www.cera- gmc.org/?action=gm_crop_database ).

[0068] Particularly useful transgenic plants which may be treated according to the invention are plants containing transformation events, or a combination of transformation events, and that are listed for example in the databases for various national or regional regulatory agencies including Event 531/ PV- GHBK04 (cotton, insect control, described in WO 2002/040677), Event 1143-14A (cotton, insect control, not deposited, described in WO 06/128569); Event 1143-51B (cotton, insect control, not deposited, described in WO 06/128570); Event 1445 (cotton, herbicide tolerance, not deposited, described in US-A 2002-120964 or WO 02/034946Event 17053 (rice, herbicide tolerance, deposited as PTA-9843, described in WO 10/117737); Event 17314 (rice, herbicide tolerance, deposited as PTA-9844, described in WO 10/117735); Event 281-24-236 (cotton, insect control - herbicide tolerance, deposited as PTA-6233, described in WO 05/103266 or US-A 2005-216969); Event 3006-210-23 (cotton, insect control - herbicide tolerance, deposited as PTA-6233, described in US-A 2007-143876 or WO 05/103266); Event 3272 (corn, quality trait, deposited as PTA-9972, described in WO 06/098952 or US-A 2006-230473); Event 33391 (wheat, herbicide tolerance, deposited as PTA-2347, described in WO 2002/027004), Event 40416 (corn, insect control - herbicide tolerance, deposited as ATCC PTA-11508, described in WO 11/075593); Event 43A47 (corn, insect control - herbicide tolerance, deposited as ATCC PTA-11509, described in WO 11/075595); Event 5307 (corn, insect control, deposited as ATCC PTA-9561, described in WO 10/077816); Event ASR-368 (bent grass, herbicide tolerance, deposited as ATCC PTA-4816, _Λ

- 74 - described in US-A 2006-162007 or WO 04/053062); Event B16 (corn, herbicide tolerance, not deposited, described in US-A 2003-126634); Event BPS-CV127-9 (soybean, herbicide tolerance, deposited as NCIMB No. 41603, described in WO 10/080829); Event BLRl (oilseed rape, restoration of male sterility, deposited as NCIMB 41193, described in WO 2005/074671), Event CE43-67B (cotton, insect control, deposited as DSM ACC2724, described in US-A 2009-217423 or WO 06/128573); Event CE44-69D

(cotton, insect control, not deposited, described in US-A 2010-0024077); Event CE44-69D (cotton, insect control, not deposited, described in WO 06/128571); Event CE46-02A (cotton, insect control, not deposited, described in WO 06/128572); Event COT102 (cotton, insect control, not deposited, described in US-A 2006-130175 or WO 04/039986); Event COT202 (cotton, insect control, not deposited, described in US-A 2007-067868 or WO 05/054479); Event COT203 (cotton, insect control, not deposited, described in WO 05/054480); ); Event DAS21606-3 / 1606 (soybean, herbicide tolerance, deposited as PTA-11028, described in WO 012/033794), Event DAS40278 (corn, herbicide tolerance, deposited as ATCC PTA-10244, described in WO 11/022469); Event DAS-44406-6 / pDAB8264.44.06.1 (soybean, herbicide tolerance, deposited as PTA-11336, described in WO 2012/075426), Event DAS- 14536-7 /pDAB8291.45.36.2 (soybean, herbicide tolerance, deposited as PTA-11335, described in WO 2012/075429), Event DAS-59122-7 (corn, insect control - herbicide tolerance, deposited as ATCC PTA 11384 , described in US-A 2006-070139); Event DAS-59132 (corn, insect control - herbicide tolerance, not deposited, described in WO 09/100188); Event DAS68416 (soybean, herbicide tolerance, deposited as ATCC PTA-10442, described in WO 11/066384 or WO 11/066360); Event DP-098140-6 (corn, herbicide tolerance, deposited as ATCC PTA-8296, described in US-A 2009-137395 or WO 08/112019); Event

DP-305423-1 (soybean, quality trait, not deposited, described in US-A 2008-312082 or WO 08/054747); Event DP-32138-1 (corn, hybridization system, deposited as ATCC PTA-9158, described in US-A 2009- 0210970 or WO 09/103049); Event DP-356043-5 (soybean, herbicide tolerance, deposited as ATCC PTA-8287, described in US-A 2010-0184079 or WO 08/002872); Event EE-1 (brinjal, insect control, not deposited, described in WO 07/091277); Event FI117 (corn, herbicide tolerance, deposited as ATCC

209031, described in US-A 2006-059581 or WO 98/044140); Event FG72 (soybean, herbicide tolerance, deposited as PTA-11041, described in WO 2011/063413), Event GA21 (corn, herbicide tolerance, deposited as ATCC 209033, described in US-A 2005-086719 or WO 98/044140); Event GG25 (corn, herbicide tolerance, deposited as ATCC 209032, described in US-A 2005-188434 or WO 98/044140); Event GHB119 (cotton, insect control - herbicide tolerance, deposited as ATCC PTA-8398, described in WO 08/151780); Event GHB614 (cotton, herbicide tolerance, deposited as ATCC PTA-6878, described in US-A 2010-050282 or WO 07/017186); Event GJ11 (corn, herbicide tolerance, deposited as ATCC 209030, described in US-A 2005-188434 or WO 98/044140); Event GM RZ13 (sugar beet, virus resistance , deposited as NCIMB-41601, described in WO 10/076212); Event H7-1 (sugar beet, herbicide tolerance, deposited as NCIMB 41158 or NCIMB 41159, described in US-A 2004-172669 or WO

04/074492); Event JOPLINl (wheat, disease tolerance, not deposited, described in US-A 2008-064032); Event LL27 (soybean, herbicide tolerance, deposited as NCIMB41658, described in WO 06/108674 or US-A 2008-320616); Event LL55 (soybean, herbicide tolerance, deposited as NCIMB 41660, described in WO 06/108675 or US-A 2008-196127); Event LLcotton25 (cotton, herbicide tolerance, deposited as ATCC PTA-3343, described in WO 03/013224 or US-A 2003-097687); Event LLRICE06 (rice, herbicide tolerance, deposited as ATCC 203353, described in US 6,468,747 or WO 00/026345); Event LLRice62 ( rice, herbicide tolerance, deposited as ATCC 203352, described in WO 2000/026345), Event LLRICE601 (rice, herbicide tolerance, deposited as ATCC PTA-2600, described in US-A 2008-2289060 or WO 00/026356); Event LY038 (corn, quality trait, deposited as ATCC PTA-5623, described in US-A 2007- 028322 or WO 05/061720); Event MIR162 (corn, insect control, deposited as PTA-8166, described in US-A 2009-300784 or WO 07/142840); Event MIR604 (corn, insect control, not deposited, described in US-A 2008-167456 or WO 05/103301); Event MON15985 (cotton, insect control, deposited as ATCC PTA-2516, described in US-A 2004-250317 or WO 02/100163); Event MON810 (corn, insect control, not deposited, described in US-A 2002-102582); Event MON863 (corn, insect control, deposited as ATCC PTA-2605, described in WO 04/011601 or US-A 2006-095986); Event MON87427 (corn, pollination control, deposited as ATCC PTA-7899, described in WO 11/062904); Event MON87460 (corn, stress tolerance, deposited as ATCC PTA-8910, described in WO 09/111263 or US-A 2011-

0138504); Event MON87701 (soybean, insect control, deposited as ATCC PTA-8194, described in US-A 2009-130071 or WO 09/064652); Event MON87705 (soybean, quality trait - herbicide tolerance, deposited as ATCC PTA-9241, described in US-A 2010-0080887 or WO 10/037016); Event MON87708 (soybean, herbicide tolerance, deposited as ATCC PTA-9670, described in WO 11/034704); Event MON87712 (soybean, yield, deposited as PTA-10296, described in WO 2012/051199), Event

MON87754 (soybean, quality trait, deposited as ATCC PTA-9385, described in WO 10/024976); Event MON87769 (soybean, quality trait, deposited as ATCC PTA-8911, described in US-A 2011-0067141 or WO 09/102873); Event MON88017 (corn, insect control - herbicide tolerance, deposited as ATCC PTA- 5582, described in US-A 2008-028482 or WO 05/059103); Event MON88913 (cotton, herbicide tolerance, deposited as ATCC PTA-4854, described in WO 04/072235 or US-A 2006-059590); Event

MON88302 (oilseed rape, herbicide tolerance, deposited as PTA-10955, described in WO 2011/153186), Event MON88701 (cotton, herbicide tolerance, deposited as PTA-11754, described in WO 2012/134808), Event MON89034 (corn, insect control, deposited as ATCC PTA-7455, described in WO 07/140256 or US-A 2008-260932); Event MON89788 (soybean, herbicide tolerance, deposited as ATCC PTA-6708, described in US-A 2006-282915 or WO 06/130436); Event MSI 1 (oilseed rape, pollination control - herbicide tolerance, deposited as ATCC PTA-850 or PTA-2485, described in WO 01/031042); Event MS8 (oilseed rape, pollination control - herbicide tolerance, deposited as ATCC PTA-730, described in WO 01/041558 or US-A 2003-188347); Event NK603 (corn, herbicide tolerance, deposited as ATCC PTA-2478, described in US-A 2007-292854); Event PE-7 (rice, insect control, not deposited, described in WO 08/114282); Event RF3 (oilseed rape, pollination control - herbicide tolerance, deposited as ATCC PTA-730, described in WO 01/041558 or US-A 2003-188347); Event RT73 (oilseed rape, herbicide tolerance, not deposited, described in WO 02/036831 or US-A 2008-070260); Event SYHT0H2 / SYN- „

- 76 -

000H2-5 (soybean, herbicide tolerance, deposited as PTA-11226, described in WO 2012/082548), Event T227-1 (sugar beet, herbicide tolerance, not deposited, described in WO 02/44407 or US-A 2009- 265817); Event T25 (corn, herbicide tolerance, not deposited, described in US-A 2001-029014 or WO 01/051654); Event T304-40 (cotton, insect control - herbicide tolerance, deposited as ATCC PTA-8171, described in US-A 2010-077501 or WO 08/122406); Event T342-142 (cotton, insect control, not deposited, described in WO 06/128568); Event TC1507 (corn, insect control - herbicide tolerance, not deposited, described in US-A 2005-039226 or WO 04/099447); Event VIP1034 (corn, insect control - herbicide tolerance, deposited as ATCC PTA-3925., described in WO 03/052073), Event 32316 (corn, insect control-herbicide tolerance, deposited as PTA-11507, described in WO 11/084632), Event 4114 (corn, insect control-herbicide tolerance, deposited as PTA-11506, described in WO 11/084621), EE- GM3 / FG72 (soybean, herbicide tolerance, ATCC Accession N° PTA-11041, WO 2011/063413A2), event DAS-68416-4 (soybean, herbicide tolerance, ATCC Accession N° PTA-10442, W02

011/066360A1), event DAS-68416-4 (soybean, herbicide tolerance, ATCC Accession N° PTA-10442, WO 2011/066384A1), event DP-040416-8 (corn, insect control, ATCC Accession N° PTA-11508, WO 2011/075593A1), event DP-043A47-3 (corn, insect control, ATCC Accession N° PTA-11509, WO 2011/075595A1), event DP-004114-3 (corn, insect control, ATCC Accession N° PTA-11506, WO 2011/084621A1), event DP-032316-8 (corn, insect control, ATCC Accession N° PTA-11507, WO 2011/084632A1), event MON-88302-9 (oilseed rape, herbicide tolerance, ATCC Accession N° PTA- 10955, WO 2011/153186A1), event DAS-21606-3 (soybean, herbicide tolerance, ATCC Accession No. PTA-11028, WO 2012/033794A2), event MON-87712-4 (soybean, quality trait, ATCC Accession N°. PTA-10296, WO 2012/051199A2), event DAS-44406-6 (soybean, stacked herbicide tolerance, ATCC Accession N°. PTA-11336, WO 2012/075426A1), event DAS-14536-7 (soybean, stacked herbicide tolerance, ATCC Accession N°. PTA-11335, WO 2012/075429A1), event SYN-000H2-5 (soybean, herbicide tolerance, ATCC Accession N°. PTA-11226, WO 2012/082548A2), event DP-061061-7 (oilseed rape, herbicide tolerance, no deposit N° available, WO 2012071039A1), event DP-073496-4 (oilseed rape, herbicide tolerance, no deposit N° available, US2012131692), event 8264.44.06.1 (soybean, stacked herbicide tolerance, Accession N° PTA-11336, WO 2012075426A2), event

8291.45.36.2 (soybean, stacked herbicide tolerance, Accession N°. PTA-11335, WO 2012075429A2), event SYHT0H2 (soybean, ATCC Accession N°. PTA-11226, WO 2012/082548A2), event MON88701 (cotton, ATCC Accession N° PTA-11754, WO 2012/134808A1), event KK179-2 (alfalfa, ATCC

Accession N° PTA-11833, WO2013003558A1), event pDAB8264.42.32.1 (soybean, stacked herbicide tolerance, ATCC Accession N° PTA-11993, WO 2013010094A1), event MZDT09Y (corn, ATCC Accession N° PTA-13025, WO 2013012775A1), event KK179-2 (alfalfa, ATCC Accession N° PTA- 11833), WO2013003558A1, event pDAB8264.42.32.1 (soybean, stacked herbicide tolerance, ATCC Accession N° PTA-1 1993), WO2013010094A1, event MZDT09Y (corn, ATCC Accession N° PTA- 13025), WO2013012775A1, event VCO-01981-5 (corn, herbicide tolerance, NCIMB Accession N° 41842), WO2013014241A1, event DAS-81419-2 X DAS-68416-4 (soybean stacked insect resistance and herbicide tolerance, ATCC Accession N° PTA- 10442), WO2013016516A1, event DAS-81419-2 (soybean stacked insect resistance and herbicide tolerance, ATCC Accession N° PTA- 12006),

WO2013016527A1, event HCEM485 (corn, herbicide tolerance, ATCC Accession N° PTA-12014), WO2013025400A1, event pDAB4468.18.07.1 (cotton, herbicide tolerance, ATCC Accession N° PTA- 12456), WO2013112525 A2, event pDAB4468.19.10.3 (cotton, herbicide tolerance, ATCC Accession N° PTA- 12457), WO2013112527A1.

[0069] In an advantageous embodiment, the compounds of the formula (I) are used for treating transgenic plants comprising at least one gene or gene fragment coding for a Bt toxin or Vip-related toxin.

[0070] Preferably, the compounds of the formula (I) are used for treating transgenic plants comprising at least one gene or gene fragment coding for a Bt toxin. A Bt toxin is a protein originating from or derived from the soil bacterium Bacillus thuringiensis which either belongs to the group of the crystal toxins (Cry) or the cytolytic toxins (Cyt). In the bacterium, they are originally formed as protoxins and are only metabolized in alkaline medium - for example in the digestive tract of certain feed insects - to their active form. There, the active toxin then binds to certain hydrocarbon structures at cell surfaces causing pores to be formed which destroy the osmotic potential of the cell, which may effect cell lysis. The result is the death of the insects. Bt toxins are active in particular against certain harmful species from the orders of the Lepidoptera (butterflies), Homoptera, Diptera and Coleoptera (beetles) in all their development stages; i.e. from the egg larva via their juvenile forms to their adult forms. [0071] It has been known for a long time that gene sequences coding for Bt toxins, parts thereof or else peptides or proteins derived from Bt toxins can be cloned with the aid of genetic engineering into agriculturally useful plants to generate transgenic plants having endogenous resistance to pests sensitive to Bt toxins. For the purpose of the invention, the transgenic plants coding for at least one Bt toxin or proteins derived therefrom are defined as "Bt plants".

[0072] The "first generation" of such Bt plants generally only comprise the genes enabling the formation of a certain toxin, thus only providing resistance to one group of pathogens. An example of a

commercially available maize variety comprising the gene for forming the CrylAb toxin is

"YieldGard®" from Monsanto which is resistant to the European corn borer. In contrast, in the Bt cotton variety (Bollgard®), resistance to other pathogens from the family of the Lepidoptera is generated by introduction by cloning of the genes for forming the Cryl Ac toxin. Other transgenic crop plants, in turn, express genes for forming Bt toxins with activity against pathogens from the order of the Coleoptera. Examples that may be mentioned are the Bt potato variety "NewLeaf®" (Monsanto) capable of forming the Cry3A toxin, which is thus resistant to the Colorado potato beetle, and the transgenic maize variety "YieldGard®" (Monsanto) which is capable of forming the Cry 3Bbl toxin and is thus protected against various species of the Western corn rootworm. „

- 78 -

[0073] In a "second generation", the multiply transgenic plants, already described above, expressing or comprising at least two foreign genes were generated.

[0074] Preference according to the invention is given to transgenic plants with Bt toxins from the group of the Cry family (see, for example, http://www.lifesci.susx.ac.uk/home/Neil_Crickmore/Bt/. [0075] Preferred are transgenic plants with Bt toxins from the group of the

NCBI Source

Acc No. NCBI Nuc Authors Year Comment

Name Protein Strain

Bt kurstaki

CrylAal AAA22353 142765 142764 Schnepf et al 1985

HD1

CrylAa2 AAA22552 551713 143100 Shibano et al 1985 Bt sotto

Bt aizawai

CrylAa3 BAA00257 216284 216283 Shimizu et al 1988

IPL7

Bt

CrylAa4 CAA31886 40267 40266 Masson et al 1989

entomocidus

Udayasuriyan et

CrylAa5 BAA04468 535781 506190 1994 Bt Fu-2-7

al

Bt kurstaki

CrylAa6 AAA86265 1171233 1171232 Masson et al 1994

NRD-12

CrylAa7 AAD46139 5669035 5669034 Osman et al 1999 Bt C12

DNA sequence

CrylAa8 126149 Liu 1996

only

Bt

CrylAa9 BAA77213 4666284 4666283 Nagamatsu et al 1999 dendrolimus

T84A1

Bt kurstaki

CrylAal 0 AAD55382 5901703 5901702 Hou and Chen 1999

HD-1-02 CrylAal l CAA70856 6687073 6687072 Tounsi et al 1999 Bt kurstaki

CrylAal2 AAP80146 32344731 32344730 Yao et al 2001 Bt Ly30

CrylAal3 AAM44305 21239436 21239435 Zhong et al 2002 Bt sotto

CrylAal4 AAP40639 37781497 37781496 Ren et al 2002 unpublished

Bt l TA

CrylAal5 AAY66993 67089177 67089176 Sauka et al 2005

Mol-12

No NCBI link

CrylAal6 HQ439776 Liu et al 2010 Bt Ps9-E2

June 13

No NCBI link

CrylAal7 HQ439788 Liu et al 2010 Bt PS9-C12

June 13

No NCBI link

CrylAal 8 HQ439790 Liu et al 2010 Bt PS9-D12

June 13

CrylAal9 HQ685121 337732098 337732097 Li & Luo 2011 Bt LS-R-21

CrylAa20 JF340156 Kumari & Kaur 2011 Bt SK-798

No NCBI link

CrylAa21 J 651496 Li Yuhong 2011 Bt LTS-209

June 13

CrylAa22 KC 158223 El Khoury et al 2013 Bt Lip

Bt berliner

CrylAbl AAA22330 142720 142719 Wabiko et al 1986

1715

CrylAb2 AAA22613 143227 143226 Thorne et al 1986 Bt kurstaki

Bt kurstaki

CrylAb3 AAA22561 143124 143123 Geiser et al 1986

HD1

Bt kurstaki

CrylAb4 BAA00071 216280 216279 Kondo et al 1987

HD1 Bt berliner

CrylAb5 CAA28405 40255 40254 Hofte et al 1986

1715

Bt kurstaki

CrylAb6 AAA22420 142886 142885 Hefford et al 1987

NRD-12

Bt aizawai

CrylAb7 CAA31620 40278 40277 Haider & Ellar 1988

IC1

Bt aizawai

CrylAb8 AAA22551 143099 143098 Oeda et al 1987

IPL7

Bt aizawai

CrylAb9 CAA38701 40273 40272 Chak & Jen 1993

HD133

Bt kurstaki

CrylAbl O A29125 Fischhoff et al 1987

HD1

DNA sequence

Cry 1 AM I 112419 Ely & Tippett 1995 Bt A20

only

Silva-Werneck Bt kurstaki

CrylAbl2 AAC64003 3746545 3746544 1998

et al S93

CrylAbl 3 AAN76494 25990352 25990351 Tan et al 2002 Bt c005

Meza-Basso & Native

CrylAbl4 AAG16877 10440886 10440885 2000

Theoduloz Chilean Bt

CrylAbl 5 AAO13302 27436100 27436098 Li et al 2001 Bt B-Hm-16

CrylAbl 6 AAK55546 14190061 14190060 Yu et al 2002 Bt AC- 1 1

CrylAbl 7 AAT46415 48734426 48734425 Huang et al 2004 Bt WB9

CrylAbl 8 AAQ88259 37048803 37048802 Stobdan et al 2004 Bt

CrylAbl 9 AAW31761 56900936 56900935 Zhong et al 2005 Bt X-2

CrylAb20 ABB72460 82395049 82395048 Liu et al 2006 BtC008 CrylAb21 ABS18384 151655610 151655609 Swiecicka et al 2007 Bt IS5056

CrylAb22 ABW87320 159024156 159024155 Wu and Feng 2008 BtS2491Ab

No NCBI link

CrylAb23 HQ439777 Liu et al 2010 Bt N32-2-2

June 13

No NCBI link

CrylAb24 HQ439778 Liu et al 2010 Bt HD12

June 13

CrylAb25 HQ685122 337732100 337732099 Li & Luo 2011 Bt LS-R-30

Prathap Reddy et

CrylAb26 HQ847729 320090245 320090244 2011 DOR BT-1

al

CrylAb27 J 135249 Ammouneh et al 2011

CrylAb28 J 135250 Ammouneh et al 2011

CrylAb29 J 135251 Ammouneh et al 2011

CrylAb30 J 135252 Ammouneh et al 2011

CrylAb31 J 135253 Ammouneh et al 2011

CrylAb32 J 135254 Ammouneh et al 2011

CrylAb33 AAS93798 Li et al 2012 Bt kenyae K3 partial cds

No NCBI link

CrylAb34 KC156668 Sampson et al 2012

June 13

CrylAb- Nagarathinam et Bt kunthala uncertain

AAK14336 13173238 13173237 2001

like al RX24 sequence

CrylAb- Nagarathinam et Bt kunthala uncertain

AAK14337 13173240 13173239 2001

like al RX28 sequence

CrylAb- Nagarathinam et Bt kunthala uncertain

AAK14338 13173242 13173241 2001

like al RX27 sequence CrylAb- insufficient

ABG88858 110734449 110734448 Lin et al 2006 Bt Iy4a3

like sequence

Bt kurstaki

CrylAcl AAA22331 Adang et al 1985

HD73

CrylAc2 AAA22338 Von Tersch et al 1991 Bt kenyae

CrylAc3 CAA38098 Dardenne et al 1990 Bt BTS89A

Bt kurstaki

CrylAc4 AAA73077 Feitelson 1991

PS85A1

Bt kurstaki

CrylAc5 AAA22339 Feitelson 1992

PS81 GG

Bt kurstaki

CrylAc6 AAA86266 Masson et al 1994

NRD-12

Bt kurstaki

CrylAc7 AAB46989 Herrera et al 1994

HD73

Bt kurstaki

CrylAc8 AAC44841 Omolo et al 1997

HD73

CrylAc9 AAB49768 Gleave et al 1992 Bt DSIR732

Bt kurstaki

Cry 1 Ac 10 CAA05505 Sun 1997

YBT-1520

Makhdoom &

CrylAcl 1 CAA10270 1998

Riazuddin

DNA sequence

CrylAcl 2 112418 Ely & Tippett 1995 Bt A20

only

Bt kurstaki

CrylAcl 3 AAD38701 Qiao et al 1999

HD1

CrylAcl 4 AAQ06607 Yao et al 2002 Bt Ly30 Bt from

Cry 1 Ac 15 AAN07788 Tzeng et al 2001

Taiwan

Cry 1 Ac 16 AAU87037 Zhao et al 2005 Bt H3

Bt kenyae

Cry 1 Ac 17 AAX18704 Hire et al 2005

HD549

Cry 1 Ac 18 AAY88347 Kaur & Allam 2005 Bt SK-729

Cry 1 Ac 19 ABD37053 Gao et al 2005 Bt C-33

CrylAc20 ABB89046 Tan et al 2005

CrylAc21 AAY66992 Sauka et al 2005 TNTA Mol-12

CrylAc22 ABZ01836 Zhang & Fang 2008 Bt W015-1

CrylAc23 CAQ30431 Kashyap et al 2008 Bt

Bt 146-158-

CrylAc24 ABL01535 Arango et al 2008

01

CrylAc25 FJ513324 237688242 237688241 Guan et al 2011 Bt Tm37-6

CrylAc26 FJ617446 256003038 256003037 Guan et al 2011 Bt Tm41-4

CrylAc27 FJ617447 256003040 256003039 Guan et al 2011 Bt Tm44-1B

CrylAc28 ACM90319 Li et al 2009 Bt Q-12

ΓΝΤΑ TA24-

CrylAc29 DQ438941 Diego Sauka 2009

6

CrylAc30 GQ227507 Zhang et al 2010 Bt S1478-l

CrylAc31 GU446674 319433505 Zhao et al 2010 Bt S3299-1

CrylAc32 HM061081 Lu et al 2010 Bt ZQ-89

CrylAc33 GQ866913 306977639 306977638 Kaur & Meena 2011 Bt SK-711

Bt

CrylBa2 CAA65003 Soetaert 1996 entomocidus

HD110

CrylBa3 AAK63251 Zhang et al 2001

Bt

CrylBa4 AAK51084 Nathan et al 2001 entomocidus

HD9

CrylBa5 ABO20894 Song et al 2007 Bt sfw-12

CrylBa6 ABL60921 Martins et al 2006 Bt S601

No NCBI link

CrylBa7 HQ439781 Liu et al 2010 Bt N17-37

June 13

CrylBbl AAA22344 Donovan et al 1994 Bt EG5847

No NCBI link

CrylBb2 HQ439782 Liu et al 2010 Bt WBT-2

June 13

CrylBcl CAA86568 Bishop et al 1994 Bt morrisoni

Bt

CrylBdl AAD 10292 Kuo et al 2000 wuhanensis

HD525

CrylBd2 AAM93496 Isakova et al 2002 Bt 834

CrylBel AAC32850 Payne et al 1998 Bt PS158C2

CrylBe2 AAQ52387 Baum et al 2003

CrylBe3 ACV96720 259156864 Sun et al 2010 Bt g9

No NCBI link

CrylBe4 HM070026 Shu et al 2010

June 13

CrylBfl CAC50778 Arnaut et al 2001 CrylBf2 AAQ52380 Baum et al 2003

CrylBgl AAO39720 Wang et al 2002

CrylBhl HQ589331 315076091 Lira et al 2010 Bt PS46L

No NCBI link

CrylBil KC156700 Sampson et al 2012

June 13

Bt

CrylCal CAA30396 Honee et al 1988 entomocidus

60.5

Bt aizawai

CrylCa2 CAA31951 Sanchis et al 1989

7.29

Bt aizawai

CrylCa3 AAA22343 Feitelson 1993

PS81I

Bt

Van Mellaert et

CrylCa4 CAA01886 1990 entomocidus

al

HD110

Bt aizawai

CrylCa5 CAA65457 Strizhov 1996

7.29

CrylCa6

AAF37224 Yu et al 2000 Bt AF-2

[1]

CrylCa7 AAG50438 Aixing et al 2000 Bt J8

CrylCa8 AAM00264 Chen et al 2001 Bt c002

CrylCa9 AAL79362 Kao et al 2003 Bt GlO-OlA

CrylCal 0 AAN16462 Lin et al 2003 Bt E05-20a

CrylCal 1 AAX53094 Cai et al 2005 Bt C-33

CrylCal2 HM070027 Shu et al 2010

No NCBI link June 13

CrylCal3 HQ412621 312192962 Li & Luo 2010 Bt LB-R-78

No NCBI link

CrylCal4 J 651493 Li Yuhong 2011 Bt LTS-38

June 13

Bt galleriae DNA sequence

CrylCbl M97880 Kalman et al 1993

HD29 only

CrylCb2 AAG35409 Song et al 2000 Bt cOOl

CrylCb3 ACD50894 Huang et al 2008 Bt 087

CrylCb- Thammasittirong insufficient

AAX63901 2005 Bt TA476-l

like et al sequence

Bt aizawai

CrylDal CAA38099 Hofte et al 1990

HD68

DNA sequence

CrylDa2 176415 Payne & Sick 1997

only

No NCBI link

CrylDa3 HQ439784 Liu et al 2010 Bt HD12

June 13

Bt

CrylDbl CAA80234 Lambert 1993

BTS00349A

CrylDb2 AAK48937 Li et al 2001 Bt B-Pr-88

Lertwiriyawong

CrylDcl ABK35074 2006 Bt JC291

et al

Bt kenyae

CrylEal CAA37933 Visser et al 1990

4F1

CrylEa2 CAA39609 Bosse et al 1990 Bt kenyae

CrylEa3 AAA22345 Feitelson 1991

Bt kenyae PS81F

Barboza-Corona Bt kenyae

CrylEa4 AAD04732 1998

et al LBIT-147

DNA sequence

CrylEa5 A15535 Botterman et al 1994

only

CrylEa6 AAL50330 Sun et al 1999 Bt YBT-032

CrylEa7 AAW72936 Huehne et al 2005 Bt JC190

CrylEa8 ABX11258 Huang et al 2007 Bt HZM2

No NCBI link

CrylEa9 HQ439785 Liu et al 2010 Bt S6

June 13

CrylEalO ADR00398 Goncalves et al 2010 Bt BR64

CrylEal l JQ652456 Lin Qunxin et al 2012 Bt

No NCBI link

CrylEal2 KF601559 Baonan He 2013 Bt strain V4

Sep 13

Bt aizawai

CrylEbl AAA22346 Feitelson 1993

PS81A2

Bt aizawai

CrylFal AAA22348 Chambers et al 1991

EG6346

Bt aizawai

CrylFa2 AAA22347 Feitelson 1993

PS81I

No NCBI link

CrylFa3 HM070028 Shu et al 2010

June 13

No NCBI link

CrylFa4 HM439638 Liu et al 2010 Bt mo3-D10

June 13

CrylFbl CAA80235 Lambert 1993

Bt

Cryllal CAA44633 Tailor et al 1992 Bt kurstaki

Crylla2 AAA22354 Gleave et al 1993 Bt kurstaki

Bt kurstaki

Crylla3 AAC36999 Shin et al 1995

HD1

Crylla4 AAB00958 Kostichka et al 1996 Bt AB88

Crylla5 CAA70124 Selvapandiyan 1996 Bt 61

Bt kurstaki

Crylla6 AAC26910 Zhong et al 1998

S101

Crylla7 AAM73516 Porcar et al 2000 Bt

Crylla8 AAK66742 Song et al 2001

Crylla9 AAQ08616 Yao et al 2002 Bt Ly30

Bt

CryllalO AAP86782 Espindola et al 2003

thuringiensis

Bt kurstaki

Cryllal 1 CAC85964 Tounsi et al 2003

BNS3

Grossi de Sa et

Cryllal2 AAV53390 2005 Bt al

Cryllal3 ABF83202 Martins et al 2006 Bt

Cryllal4 ACG63871 Liu & Guo 2008 Btl l

Cryllal5 FJ617445 256003036 256003035 Guan et al 2011 Bt E-1B

Cryllal6 FJ617448 256003042 256003041 Guan et al 2011 Bt E-1A

Cryllal7 GU989199 Li et al 2010 Bt MX2

Cryllal 8 ADK23801 300492624 Li et al 2010 Bt MX9 No NCBI link

Cryllal9 HQ439787 Liu et al 2010 Bt SC6H6

June 13

No NCBI link

Crylla20 JQ228426 Zhao Can 2011 Bt wulH-3

June 13

No NCBI link

Crylla21 JQ228424 2011 Bt youlD-9

Zhao Can June 13

No NCBI link

Crylla22 JQ228427 Zhao Can 2011 Bt wulE-3

June 13

No NCBI link

Crylla23 JQ228428 Zhao Can 2011 Bt wulE-4

June 13

No NCBI link

Crylla24 JQ228429 Zhao Can 2011 Bt wu2B-6

June 13

No NCBI link

Crylla25 JQ228430 Zhao Can 2011 Bt wu2G-l l

June 13

No NCBI link

Crylla26 JQ228431 Zhao Can 2011 Bt wu2G-12

June 13

No NCBI link

Crylla27 JQ228432 Zhao Can 2011 Bt you2D-3

June 13

No NCBI link

Crylla28 JQ228433 Zhao Can 2011 Bt you2E-3

June 13

No NCBI link

Crylla29 JQ228434 Zhao Can 2011 Bt you2F-3

June 13

Crylla30 JQ317686 Kumari & Kaur 2011 Bt 4J4

Crylla31 JX944038 Song et al 2012 Bt SC-7

Crylla32 JX944039 Song et al 2012 Bt SC-13

Crylla33 JX944040 Song et al 2012 Bt SC-51 Bt

Cryllbl AAA82114 Shin et al 1995 entomocidus

BP465

Cryllb2 ABW88019 Guan et al 2007 Bt PP61

Cryllb3 ACD75515 Liu & Guo 2008 Bt GS8

Cryllb4 HM051227 301641366 Zhao et al 2010 Bt BF-4

No NCBI link

Cryllb5 HM070028 Shu et al 2010

June 13

Cryllb6 ADK38579 300836937 Li et al 2010 Bt LB52

Cryllb7 JN571740 Kumari & Kaur 2011 Bt SK-935

Cryllb8 JN675714 Swamy et al 2011

Cryllb9 JN675715 Swamy et al 2011

Cry lib 10 JN675716 Swamy et al 2011

No NCBI link

Cryllbl 1 JQ228423 Zhao Can 2011 Bt HD12

June 13

Cryllcl AAC62933 Osman et al 1998 Bt C18

Cryllc2 AAE71691 Osman et al 2001

Crylldl AAD44366 Choi 2000

No NCBI link

Crylld2 JQ228422 Zhao Can 2011 Bt HD12

June 13

Cryllel AAG43526 Song et al 2000 Bt BTC007

No NCBI link

Crylle2 HM439636 Liu et al 2010 Bt T03B001

June 13 No NCBI link

Crylle3 KC 156647 Sampson et al 2012

June 13

No NCBI link

Crylle4 KC156681 Sampson et al 2012

June 13

Cryllfl AAQ52382 Baum et al 2003

No NCBI link

Cryllgl KC 156701 Sampson et al 2012

June 13 insufficient

Cryll-like AAC31094 Payne et al 1998

sequence insufficient

Cryll-like ABG88859 Lin & Fang 2006 Bt Iy4a3

sequence

CrylJal AAA22341 Donovan 1994 Bt EG5847

No NCBI link

CrylJa2 HM070030 Shu et al 2010

June 13

No NCBI link

CrylJa3 JQ228425 Zhao Shiyuan 2011 Bt FH21

June 13

Von Tersch &

CrylJbl AAA98959 1994 Bt EG5092

Gonzalez

CrylJcl AAC31092 Payne et al 1998

CrylJc2 AAQ52372 Baum et al 2003

CrylJdl CAC50779 Arnaut et al 2001 Bt

Bt morrisoni

CrylKal AAB00376 Koo et al 1995

BF190

No NCBI link

CrylKa2 HQ439783 Liu et al 2010 Bt WBT-2

June 13 Bt kurstaki

CrylLal AAS60191 Je et al 2004

Kl

No NCBI link

CrylLa2 HM070031 Shu et al 2010

June 13

Noguera &

CrylMal FJ884067 2010 LBIT 1189

Ibarra

No NCBI link

CrylMa2 KC156659 Sampson et al 2012

June 13

No NCBI link

CrylNal KC156648 Sampson et al 2012

June 13

No NCBI link

CrylNbl KC156678 Sampson et al 2012

June 13

[0076] Particular preference is given to the genes or gene sections of the subfamilies cryl, cry2, cry3, cry5 and cry9; especially preferred are members of the subfamily crylA such as crylAa, crylAc, cry2Ab.

[0077] Furthermore, it is preferred to use plants which, in addition to the genes for one or more Bt toxins, express or contain, if appropriate, also genes for expressing, for example, a protease or peptidase inhibitor (such as in WO-A 95/35031), of herbicide resistances (for example to glufosinate or glyphosate by expression of the pat gene or bar gene) or for becoming resistant to nematodes, fungi or viruses (for example by expressing a gluconase, chitinase). However, they may also be genetically modified in their metabolic properties, so that they show a qualitative and/or quantitative change of ingredients (for example by modification of the energy, carbohydrate, fatty acid or nitrogen metabolism or by metabolite currents influencing these (see above).

[0078] In one preferred embodiment, a Bt-plant, preferably a Bt-soybean, comprises event MON87701 which is described in, e.g., WO2009/064652. Thus, in one preferred embodiment, a Bt-soybean seeds comprising said event of which a representative sample was deposited at the ATCC under Accession No. PTA-8194 are treated with a ryanodine receptor modulator according to the present invention.

[0079] In another preferred embodiment, a Bt-soybean comprises event pDAB9582.814.19.1 and/or event pDAB4468.04.16.1 which are described in, e.g., WO 2013/016516. This breeding stacks comprise crylF, crylAc and pat and aad-12 and pat, as described in WO 2012/075426. Thus, in one preferred ^ embodiment, a Bt-soybean seeds of which comprising said events were deposited at the ATCC under Accession No. PTA-10442 (pDAB4468.04.16.1) are treated with a ryanodine receptor modulator according to the present invention.

[0080] In one preferred embodiment, the method of the invention is characterized in that the Bt-plant, preferably a Bt-soybean plant, comprises at least one cry-gene or a cry-gene fragment coding for a Bt toxin.

[0081] In one preferred embodiment, said method is characterized in that the Bt-plant, preferably Bt- soybean plant, comprises at least one cryl A-gene or cryl A-gene fragment coding for a Bt toxin.

[0082] In one preferred embodiment, said method is characterized in that said Bt-plant, preferably Bt- soybean plant, further comprising a cryF gene or cryF-gene fragment coding for a Bt toxin.

[0083] In another preferred embodiment, said method is characterized in that said plant, preferably said soybean plant, comprises event MON87701.

[0084] In a more preferred embodiment, said soybean plant comprises event MON87701 and event MON89788, e.g. Intacta™ Roundup Ready™ 2 Pro. [0085] In another preferred embodiment, said method is characterized in that said soybean plant comprising DNA that comprises a first sequence selected from the group consisting of bp 1385-1415 of SEQ ID NO: 1; bp 1350-1450 of SEQ ID NO: l ; bp 1300-1500 of SEQ ID NO: l ; bp 1200-1600 of SEQ ID NO: 1; bp 137- 168 of SEQ ID NO:2; bp 103-203 of SEQ ID NO:2; and bp 3-303 of SEQ ID NO:2; and a second sequence selected from the group consisting bp 2680-2780 of SEQ ID NO: 3; bp 2630-2830 of SEQ ID NO: 15; bp 2530-2930 of SEQ ID NO: 15; bp 9071-9171 of SEQ ID NO : 15 ; bp 9021 -9221 of SEQ ID NO : 15 ; and, bp 8921 -9321 of SEQ ID NO : 15 said first and second sequences being diagnostic for the presence of soybean event pDAB9582.814.19.1 :: pDAB4468.04.16.1.

pDAB9582.814.19.1 :: pDAB4468.04.16.1 are disclosed in WO 2013/016516.

[0086] In one preferred embodiment, said method is characterized in that said soybean plant comprising a nucleotide sequence of SEQ ID NO: 4, SEQ ID NO:5, or complement thereof.

[0087] In one preferred embodiment, said method is characterized in that said soybean plant comprising a nucleotide sequence of SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 9 or complement thereof.

[0088] In one preferred embodiment, said method is characterized in that said soybean plant comprising a nucleotide sequence of SEQ ID NO:6 from positions 1 to 5757, the nucleotide sequence of SEQ ID NO: 8 from positions 1 to 6426, and the nucleotide sequence of SEQ ID NO: 7 from positions 379 to 2611, or complement thereof. ^

- 96 -

[0089] In one preferred embodiment, said method is characterized in that said soybean plant comprising a nucleotide sequence essentially of the nucleotide sequence of SEQ ID NO: 9 or complement thereof.

[0090] In another preferred embodiment, said method is characterized in that said pest is selected from the group consisting of Pseudoplusia includens (soybean looper), Anticarsia gemmatalis (velvet bean caterpillar) and Spodoptera frugiperda (fall armyworm).

[0091] In another preferred embodiment, said method is characterized in that the use form of the ryanodine receptor modulator is present in a mixture with at least one mixing partner.

[0092] A second aspect refers to a method for improving the utilization of the production potential of transgenic soybean plants in the absent of a pest. Preferred embodiments of this aspect are identical to the preferred embodiments disclosed for the first aspect of the present invention.

[0093] A third aspect refers to a synergistic composition comprising Bt toxins encoded by a nucleotide sequence that comprises a first sequence selected from the group consisting of bp 1385-1415 of SEQ ID NO: 1; bp 1350- 1450 of SEQ ID NO: 1 ; bp 1300-1500 of SEQ ID NO: 1 ; bp 1200-1600 of SEQ ID NO: 1; bp 137- 168 of SEQ ID NO:2; bp 103-203 of SEQ ID NO:2; and bp 3-303 of SEQ ID NO:2; and a second sequence selected from the group consisting bp 2680-2780 of SEQ ID NO: 3; bp 2630- 2830 of SEQ ID NO: 15; bp 2530-2930 of SEQ ID NO: 15; bp 9071-9171 of SEQ ID NO : 15 ; bp 9021 -9221 of SEQ ID NO : 15 ; and, bp 8921 -9321 of SEQ ID NO : 15 or a nucleotide sequence of SEQ ID NO: 4, SEQ ID NO:5, or complement thereof and a ryanodine receptor modulator as described herein.

[0094] A fourth aspect refers to a Bt-soybean plant, characterized in that at least 0.00001 g of a ryanodine receptor modulator as described herein is attached to it.

[0095] SEQ ID No: l(disclosed in WO 2013/016516) is the 5' DNA flanking border sequence for soybean event pDAB9582.814.19.1. Nucleotides 1-1400 are genomic sequence. Nucleotides 1401-1535 are a rearranged sequence from pDAB9582. Nucleotides 1536-1836 are insert sequence.

[0096] SEQ ID No: 2 (disclosed in WO 2013/016516) is the 3' DNA flanking border sequence for soybean event pDAB9582.814.19.1. Nucleotides 1-152 are insert sequence. Nucleotides 153-1550 are genomic sequence. „

- 97 -

[0097] SEQ ID No: 3 (disclosed in WO 2013/016516) is the confirmed sequence of soybean event pDAB4468.04.16.1. Including the 5' genomic flanking sequence, pDAB4468 T-strand insert, and 3' genomic flanking sequence.

[0098] SEQ ID No:4 (disclosed in WO 2009/064652) is a A 20 nucleotide sequence representing the junction between the soybean genomic DNA and an integrated expression cassette. This sequence corresponds to positions 5748 to 5767 of SEQ ID NO:9. In addition, SEQ ID NO: 1 is a nucleotide sequence corresponding to positions 5748 through 5757 of SEQ ID NO:6 and the integrated right border of the TIC 107 expression cassette corresponding to positions 1 through 10 of SEQ ID NO: 8. SEQ ID NO: l also corresponds to positions 5748 to 5767 of the 5' flanking sequence, SEQ ID NO:6. [0099] SEQ ID No: 5 (disclosed in WO 2009/064652) is a 20 nucleotide sequence representing the junction between an integrated expression cassette and the soybean genomic DNA. This sequence corresponds to positions 12174 to 12193 of SEQ ID NO:9. In addition, SEQ ID NO:2 is a nucleotide sequence corresponding positions 6417 through 6426 of SEQ ID NO: 8 and the 3' flanking sequence corresponding to positions 379 through 388 of SEQ ED NO:7. [0100] SEQ ID No: 6 (disclosed in WO 2009/064652) is the 5' sequence flanking the inserted DNA of MON87701 up to and including a region of transformation DNA (T-DNA) insertion.

[0101] SEQ ID No: 7 (disclosed in WO 2009/064652) is the 3' sequence flanking the inserted DNA of MON87701 up to and including a region of T-DNA insertion.

[0102] SEQ ID No: 8 (disclosed in WO 2009/064652) is the sequence of the integrated TIC 107 expression cassette, including right and left border sequence after integration.

[0103] SEQ ID No: 9 (disclosed in WO 2009/064652) is a 14,416 bp nucleotide sequence representing the contig of the 5' sequence flanking the inserted DNA of MON87701 (SEQ ID NO:6), the sequence of the integrated expression cassette (SEQ ID NO:8) and the 3' sequence flanking the inserted DNA of MON87701 (SEQ ID NO: 7). [0104] A nucleic acid molecule is said to be the "complement" of another nucleic acid molecule if they exhibit complete complementarity. As used herein, molecules are said to exhibit "complete

complementarity" when every nucleotide of one of the molecules is complementary to a nucleotide of the other. Two molecules are said to be "minimally complementary" if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under at least conventional "low- stringency" conditions. Similarly, the molecules are said to be "complementary" if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under conventional "high-stringency" conditions. Conventional stringency conditions are described by Sambrook et al, 1989, and by Haymes et al, In: Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, DC (1985), Departures from complete complementarity are therefore permissible, as long as such departures do not completely preclude the capacity of the molecules to form a double-stranded structure. In order for a nucleic acid molecule to serve as a primer or probe it need only be sufficiently complementary in sequence to be able to form a stable double-stranded structure under the particular solvent and salt concentrations employed.

[0105] As used herein, a "substantially homologous sequence" is a nucleic acid sequence that will specifically hybridize to the complement of the nucleic acid sequence to which it is being compared under high stringency conditions. Appropriate stringency conditions which promote DNA hybridization, for example, 6.0 x sodium chloride/sodium citrate (SSC) at about 45<0>C, followed by a wash of 2.0 x SSC at 50<0>C, are known to those skilled in the art or can be found in Current Protocols in Molecular

Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. For example, the salt concentration in the wash step can be selected from a low stringency of about 2.0 x SSC at 50<0>C to a high stringency of about 0.2 x SSC at 5O<0>C. In addition, the temperature in the wash step can be increased from low stringency conditions at room temperature, about 22<0>C, to high stringency conditions at about 65<0>C. Both temperature and salt may be varied, or either the temperature or the salt concentration may be held constant while the other variable is changed. In a preferred embodiment, a nucleic acid of the present invention will specifically hybridize to one or more of the nucleic acid molecules set forth in SEQ ID NO: 1 and 2 or complements thereof or fragments of either under moderately stringent conditions, for example at about 2.0 x SSC and about 65<0>C. In a particularly preferred embodiment, a nucleic acid of the present invention will specifically hybridize to one or more of the nucleic acid molecules set forth in SEQ ID NO: 1 and SEQ ID NO:2 or complements or fragments of either under high stringency conditions. In one aspect of the present invention, a preferred marker nucleic acid molecule of the present invention has the nucleic acid sequence set forth in SEQ ID NO: l and SEQ ID NO:2 or complements thereof or fragments of either. In another aspect of the present invention, a preferred marker nucleic acid molecule of the present invention shares 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%,

92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% and 100% sequence identity with the nucleic acid sequence set forth in SEQ ID NO: l and SEQ ID NO:2 or complement thereof or fragments of either. In a further aspect of the present invention, a preferred marker nucleic acid molecule of the present invention shares 95% 96%, 97%, 98%, 99% and 100% sequence identity with the sequence set forth in SEQ ID NO: l and SEQ ID NO: 2 or complement thereof or fragments of either. SEQ ID NO:l and SEQ ID NO:2 may be used as markers in plant breeding methods to identify the progeny of genetic crosses similar to the methods described for simple sequence repeat DNA marker analysis, in "DNA markers: Protocols, applications, and overviews: (1997) 173-185, Cregan, et al., eds., Wiley-Liss NY"; all of which is herein incorporated by reference. The hybridization of the probe to the target DNA molecule can be detected by any number of methods known to those skilled in the art, these can include, but are not limited to, fluorescent tags, radioactive tags, antibody based tags, and chemilluminescent tags.

[0064] Regarding the amplification of a target nucleic acid sequence (e.g., by PCR) using a particular amplification primer pair, "stringent conditions" are conditions that permit the primer pair to hybridize only to the target nucleic-acid sequence to which a primer having the corresponding wild-type sequence (or its complement) would bind and preferably to produce a unique amplification product, the amplicon, in a DNA thermal amplification reaction. [0065] The term "specific for (a target sequence)" indicates that a probe or primer hybridizes under stringent hybridization conditions only to the target sequence in a sample comprising the target sequence.

[0106] In a particularly preferred variant, the process according to the invention is used for treating transgenic vegetable, maize, soya bean, cotton, tobacco, rice, potato and sugar beet varieties. These are preferably Bt plants.

[0107] The vegetable plants or varieties are, for example, the following useful plants: o potatoes: preferably starch potatoes, sweet potatoes and table potatoes; o root vegetables: preferably carrots, turnips (swedes, stubble turnips {Brassica rapa var. rapa), spring turnips, autumn turnips {Brassica campestris ssp. rapifera), Brassica rapa L. ssp. rapa f. teltowiensis), scorzonera, Jerusalem artichoke, turnip-rooted parsley, parsnip, radish and horseradish; o tuber vegetables: preferably kohlrabi, beetroot, celeriac, garden radish; o bulb crops: preferably scallion, leek and onions (planting onions and seed onions); o brassica vegetables: preferably headed cabbage (white cabbage, red cabbage, kale, savoy cabbage), cauliflowers, broccoli, curly kale, marrow-stem kale, seakale and Brussels sprouts; o fruiting vegetables: preferably tomatoes (outdoor tomatoes, vine-ripened tomatoes, beef tomatoes, greenhouse tomatoes, cocktail tomatoes, industrial and fresh market tomatoes), melons, eggplants, aubergines, pepper (sweet pepper and hot pepper, Spanish pepper), chilli pepper, pumpkins, courgettes and cucumbers (outdoor cucumbers, greenhouse cucumbers snake gourds and gherkins); o vegetable pulses: preferably bush beans (as sword beans, string beans, flageolet beans, wax beans, corn beans of green- and yellow-podded cultivars), pole beans (as sword beans, string beans, flageolet beans, wax beans of green-, blue- and yellow-podded cultivars), broadbeans (field beans, Windsor beans, cultivars having white- and black-spotted flowers), peas (chickling vetch, chickpeas, marrow peas, shelling peas, sugar-peas, smooth peas, cultivars having light- and dark-green fresh fruits) and lentils; o green vegetables and stem vegetables: preferably Chinese cabbage, round-headed garden lettuce, curled lettuce, lamb's-lettuce, iceberg lettuce, romaine lettuce, oakleaf lettuce, endives, radicchio, lollo rossa, ruccola lettuce, chicory, spinach, chard (leaf chard and stem chard) and parsley; o other vegetables: preferably asparagus, rhubarb, chives, artichokes, mint varieties, sunflowers, Florence fennel, dill, garden cress, mustard, poppy seed, peanuts, sesame and salad chicory.

[0108] Bt vegetables including exemplary methods for preparing them are described in detail, for example, in Barton et al., 1987, Plant Physiol. 85 : 1103-1109; Vaeck et al., 1987, Nature 328 : 33-37 ; Fischhoff et al., 1987, Bio/Technology 5 : 807-813. In addition, Bt vegetable plants are already known as commercial varieties, for example the potato cultivar NewLeaf® (Monsanto). The preparation of Bt vegetables is also described in US 6,072,105.

[0109] Likewise, Bt cotton is already known in principle, for example from US-A-5, 322,938. In the context of the present invention, particular preference is given to Bt cotton with the trade names

NuCOTN33® and NuCOTN33B®.

[0110] The use and preparation of Bt maize has likewise already been known for a long time, for example from Ishida, Y., Saito, H., Ohta, S., Hiei, Y., Komari, T., and Kumashiro, T. (1996). High efficiency transformation of maize (Zea mayz L.) mediated by Agrobacterium tumefaciens, Nature Biotechnology 4: 745-750. EP-B-0485506, too, describes the preparation of Bt maize plants.

Furthermore, different varieties of Bt maize are commercially available, for example under the following names (company/companies is/are in each case given in brackets): KnockOut® (Novartis Seeds), NaturGard® (Mycogen Seeds), Yieldgard® (Novartis Seeds, Monsanto, Cargill, Golden Harvest, Pioneer, DeKalb inter alia), Bt-Xtra® (DeKalb) and StarLink® (Aventis CropScience, Garst inter alia). For the purpose of the present invention, particular preference is given especially to the following maize cultivars: KnockOut®, NaturGard®, Yieldgard®, Bt-Xtra® and StarLink®.

[0111] For soya beans, too, Roundup®Ready cultivar or cultivars resistant to the herbicide Liberty Link® are available and can be treated according to the invention. In the case of rice, a large number of "Golden Rice" lines are available which are likewise characterized in that, by virtue of a transgenic modification, they have an increased content of provitamin A. They, too, are examples of plants which can be treated by the method according to the invention, with the advantages described.

[0112] The method according to the invention is suitable for controlling a large number of harmful organisms which occur in particular in vegetables, maize and cotton, in particular insects and arachnids, very particularly preferably insects. The pests mentioned include: o From the order of the Anoplura (Phthiraptera),, for example, Damalinia spp., Haematopinus spp., Linognathus spp., Pediculus spp., Trichodectes spp.. o From the class of the Arachnida, for example, Acarus siro, Aceria sheldoni, Aculops spp., Aculus spp., Amblyomma spp., Argas spp., Boophilus spp., Brevipalpus spp., Bryobia praetiosa, Chorioptes spp., Dermanyssus gallinae, Eotetranychus spp., Epitrimerus pyri, Eutetranychus spp., Eriophyes spp., Hemitarsonemus spp., Hyalomma spp., Ixodes spp., Latrodectus mactans, Metatetranychus spp., Oligonychus spp., Ornithodoros spp., Panonychus spp., Phyllocoptruta oleivora, Polyphagotarsonemus latus, Psoroptes spp., Rhipicephalus spp., Rhizoglyphus spp., Sarcoptes spp., Scorpio maurus, Stenotarsonemus spp., Tarsonemus spp., Tetranychus spp., Vasates lycopersici. o From the class of the Bivalva, for example, Dreissena spp.. o From the order of the Chilopoda, for example, Geophilus spp., Scutigera spp.. o From the order of the Coleoptera, for example, Acanthoscelides obtectus, Adoretus spp., Agelastica alni, Agriotes spp., Amphimallon solstitialis, Anobium punctatum, Anoplophora spp., Anthonomus spp., Anthrenus spp., Apogonia spp., Atomaria spp., Attagenus spp., Bruchidius obtectus, Bruchus spp., Ceuthorhynchus spp., Cleonus mendicus, Conoderus spp., Cosmopolites spp., Costelytra zealandica, Curculio spp., Cryptorhynchus lapathi, Dermestes spp., Diabrotica spp., Epilachna spp., Faustinus cubae, Gibbium psylloides, Heteronychus arator, Hylamorpha elegans, Hylotrupes bajulus, Hypera postica, Hypothenemus spp., Lachnosterna consanguinea, Leptinotarsa decemlineata, Lissorhoptrus oryzophilus, Lixus spp., Lyctus spp., Meligethes aeneus, Melolontha melolontha, Migdolus spp., Monochamus spp., Naupactus xanthographus, Niptus hololeucus, Oryctes rhinoceros, Oryzaephilus surinamensis, Otiorrhynchus sulcatus, Oxycetonia jucunda, Phaedon cochleariae, Phyllophaga spp., Popillia japonica, Premno- trypes spp., Psylliodes chrysocephala, Ptinus spp., Rhizobius ventralis, Rhizopertha dominica, Sitophilus spp., Sphenophorus spp., Sternechus spp., Symphyletes spp., Tenebrio molitor, Tribolium spp., Trogoderma spp., Tychius spp., Xylotrechus spp., Zabrus spp.. o From the order of the Collembola, for example, Onychiurus armatus. o From the order of the Dermaptera, for example, Forficula auricularia. o From the order of the Diplopoda, for example, Blaniulus guttulatus. o From the order of the Diptera, for example, Aedes spp., Anopheles spp., Bibio hortulanus, Calliphora erythrocephala, Ceratitis capitata, Chrysomyia spp., Cochliomyia spp., Cordylobia anthropophaga, Culex spp., Cuterebra spp., Dacus oleae, Dermatobia hominis, Drosophila spp., Fannia spp., Gastrophilus spp., Hylemyia spp., Hyppobosca spp., Hypo- derma spp., Liriomyza spp.. Lucilia spp., Musca spp., Nezara spp., Oestrus spp., Oscinella frit, Pegomyia hyoscyami, Phorbia spp., Stomoxys spp., Tabanus spp., Tannia spp., Tipula paludosa, Wohlfahrtia spp.. o From the class of the Gastropoda, for example, Arion spp., Biomphalaria spp., Bulinus spp., Deroceras spp., Galba spp., Lymnaea spp., Oncomelania spp., Succinea spp.. o From the class of the helminths, for example, Ancylostoma duodenale, Ancylostoma ceylanicum, Acylostoma braziliensis, Ancylostoma spp., Ascaris lubricoides, Ascaris spp.,

Brugia malayi, Brugia timori, Bunostomum spp., Chabertia spp., Clonorchis spp., Cooperia spp., Dicrocoelium spp, Dictyocaulus filaria, Diphyllobothrium latum, Dracunculus medinensis, Echinococcus granulosus, Echinococcus multilocularis, Enterobius vermicularis, Faciola spp., Haemonchus spp., Heterakis spp., Hymenolepis nana, Hyostrongulus spp., Loa Loa, Nematodirus spp., Oesophagostomum spp., Opisthorchis spp., Onchocerca volvulus, Ostertagia spp., Paragonimus spp., Schistosomen spp, Strongyloides fuelleborni, Strongyloides stercoralis, Stronyloides spp., Taenia saginata, Taenia solium, Trichinella spiralis, Trichinella nativa, Trichinella britovi, Trichinella nelsoni, Trichinella pseudopsiralis, Trichostrongulus spp., Trichuris trichuria, Wuchereria bancrofti. o It is furthermore possible to control Protozoa, such as Eimeria. o From the order of the Heteroptera, for example, Anasa tristis, Antestiopsis spp., Blissus spp., Calocoris spp., Campylomma livida, Cavelerius spp., Cimex spp., Creontiades dilutus, Dasynus piperis, Dichelops furcatus, Diconocoris hewetti, Dysdercus spp., Euschistus spp., Eurygaster spp., Heliopeltis spp., Horcias nobilellus, Leptocorisa spp.,

Leptoglossus phyllopus, Lygus spp., Macropes excavatus, Miridae, Nezara spp., Oebalus spp., Pentomidae, Piesma quadrata, Piezodorus spp., Psallus seriatus, Pseudacysta persea, Rhodnius spp., Sahlbergella singularis, Scotinophora spp., Stephanitis nashi, Tibraca spp., Triatoma spp.. o From the order of the Homoptera, for example, Acyrthosipon spp., Aeneolamia spp.,

Agonoscena spp., Aleurodes spp., Aleurolobus barodensis, Aleurothrixus spp., Amrasca spp., Anuraphis cardui, Aonidiella spp., Aphanostigma piri, Aphis spp., Arboridia apicalis, Aspidiella spp., Aspidiotus spp., Atanus spp., Aulacorthum solani, Bemisia spp., Brachycaudus helichrysii, Brachycolus spp., Brevicoryne brassicae, Calligypona marginata, Carneocephala fulgida, Ceratovacuna lanigera, Cercopidae, Ceroplastes spp., Chaetosiphon fragaefolii, Chionaspis tegalensis, Chlorita onukii, Chromaphis juglandicola, Chrysomphalus ficus, Cicadulina mbila, Coccomytilus halli, Coccus spp., Cryptomyzus ribis, Dalbulus spp., Dialeurodes spp., Diaphorina spp., Diaspis spp., Doralis spp., Drosicha spp., Dysaphis spp., Dysmicoccus spp., Empoasca spp., Eriosoma spp., Erythroneura spp., Euscelis bilobatus, Geococcus coffeae, Homalodisca coagulata, Hyalopterus arundinis, Icerya spp., Idiocerus spp., Idioscopus spp., Laodelphax striatellus, Lecanium spp., Lepidosaphes spp., Lipaphis erysimi, Macrosiphum spp., Mahanarva fimbriolata, Melanaphis sacchari, Metcalfiella spp., Metopolophium dirhodum, Monellia costalis, Monelliopsis pecanis, Myzus spp., Nasonovia ribisnigri, Nephotettix spp., Nilaparvata lugens, Oncometopia spp., Orthezia praelonga, Parabemisia myricae, Paratrioza spp., Parlatoria spp., Pemphigus spp., Peregrinus maidis, Phenacoccus spp., Phloeomyzus passerinii, Phorodon humuli, Phylloxera spp., Pinnaspis aspidistrae, Planococcus spp., Protopulvinaria pyriformis, Pseudaulacaspis pentagona, Pseudococcus spp., Psylla spp., Pteromalus spp., Pyrilla spp., Quadraspidiotus spp., Quesada gigas, Rastrococcus spp., Rhopalosiphum spp., Saissetia spp., Scaphoides titanus, Schizaphis graminum, Selenaspidus articulatus, Sogata spp., Sogatella furcifera, Sogatodes spp., Stictocephala festina, Tenalaphara malayensis, Tinocallis caryaefoliae, Tomaspis spp., Toxoptera spp., Trialeurodes vaporariorum, Trioza spp., Typhlocyba spp., Unaspis spp., Viteus vitifolii..

From the order of the Hymenoptera, for example, Diprion spp., Hoplocampa spp., Lasius spp., Monomorium pharaonis, Vespa spp..

From the order of the Isopoda, for example, Armadillidium vulgare, Oniscus asellus, Por- cellio scaber.

From the order of the Isoptera, for example, Reticulitermes spp., Odontotermes spp..

From the order of the Lepidoptera, for example, Acronicta major, Aedia leucomelas, Agrotis spp., Alabama argillacea, Anticarsia spp., Barathra brassicae, Bucculatrix thur- beriella, Bupalus piniarius, Cacoecia podana, Capua reticulana, Carpocapsa pomonella, Cheimatobia brumata, Chilo spp., Choristoneura fumiferana, Clysia ambiguella, Cnaphalo- cerus spp., Earias insulana, Ephestia kuehniella, Euproctis chrysorrhoea, Euxoa spp., Feltia spp., Galleria mellonella, Helicoverpa spp., Heliothis spp., Hofmannophila pseudospretella, Homona magnanima, Hyponomeuta padella, Laphygma spp., Lithocolletis blancardella, Lithophane antennata, Loxagrotis albicosta, Lymantria spp., Malacosoma neustria, Mame- stra brassicae, Mocis repanda, Mythimna separata, Oria spp., Oulema oryzae, Panolis flammea, Pectinophora gossypiella, Phyllocnistis citrella, Pieris spp., Plutella xylostella, Prodenia spp., Pseudaletia spp., Pseudoplusia includens, Pyrausta nubilalis, Spodoptera spp., Thermesia gemmatalis, Tinea pellionella, Tineola bisselliella, Tortrix viridana, Trichoplusia spp.. o From the order of the Orthoptera, for example, Acheta domesticus, Blatta orientalis, Blattella germanica, Gryllotalpa spp., Leucophaea maderae, Locusta spp., Melanoplus spp., Periplaneta americana, Schistocerca gregaria. o From the order of the Siphonaptera, for example, Ceratophyllus spp., Xenopsylla cheopis. o From the order of the Symphyla, for example, Scutigerella immaculata. o From the order of the Thysanoptera, for example, Baliothrips biformis, Enneothrips flavens, Frankliniella spp., Heliothrips spp., Hercinothrips femoralis, Kakothrips spp., Rhipiphorothrips cruentatus, Scirtothrips spp., Taeniothrips cardamoni, Thrips spp.. o From the order of the Thysanura, for example, Lepisma saccharina. o The phytoparasitic nematodes include, for example, Anguina spp., Aphelenchoides spp., Belonoaimus spp., Bursaphelenchus spp., Ditylenchus dipsaci, Globodera spp., Heliocotylenchus spp., Heterodera spp., Longidorus spp., Meloidogyne spp., Pratylenchus spp., Radopholus similis, Rotylenchus spp., Trichodorus spp., Tylenchorhynchus spp., Tylenchulus spp., Tylenchulus semipenetrans, Xiphinema spp..

[0113] The method according to the invention for the treatment of Bt vegetables, Bt maize, Bt cotton, Bt soya beans, Bt tobacco and also Bt rice, Bt sugar beets or Bt potatoes is particularly suitable for controlling aphids (Aphidina), whiteflies (Trialeurodes), thrips (Thysanoptera), spider mites (Arachnida), soft scale insects or mealy bugs (Coccoidae and Pseudococcoidae, respectively).

[0114] The active compounds which can be used according to the invention can be employed in customary formulations, such as solutions, emulsions, wettable powders, water- and oil-based

suspensions, powders, dusts, pastes, soluble powders, soluble granules, granules for broadcasting, suspoemulsion concentrates, natural compounds impregnated with active compound, synthetic substances impregnated with active compound, fertilizers and also microencapsulations in polymeric substances.

[0115] These formulations are prepared in a known manner, for example by mixing the active compounds with extenders, i.e. liquid solvents and/or solid carriers, if appropriate using surfactants, i.e. emulsifiers and/or dispersants and/or foam-formers. The formulations are prepared either in suitable plants or else before or during application. [0116] Wettable powders are preparations which can be dispersed homogeneously in water and which, in addition to the active compound and beside a diluent or inert substance, also comprise wetting agents, for example polyethoxylated alkylphenols, polyethoxylated fatty alcohols, alkylsulphonates or

alkylphenylsulphonates and dispersants, for example sodium lignosulphonate, sodium 2,2'- dinaphthylmethane-6,6'-disulphonate.

[0117] Dusts are obtained by grinding the active compound with finely distributed solid substances, for example talc, natural clays, such as kaolin, bentonite, pyrophillite or diatomaceous earth. Granules can be prepared either by spraying the active compound onto granular inert material capable of adsorption or by applying active compound concentrates to the surface of carrier substances, such as sand, kaolinites or granular inert material, by means of adhesives, for example polyvinyl alcohol, sodium polyacrylate or mineral oils. Suitable active compounds can also be granulated in the manner customary for the preparation of fertilizer granules - if desired as a mixture with fertilizers.

[0118] Suitable for use as auxiliaries are substances which are suitable for imparting to the composition itself and/or to preparations derived therefrom (for example spray liquors, seed dressings) particular properties such as certain technical properties and/or also particular biological properties. Typical suitable auxiliaries are: extenders, solvents and carriers.

[0119] Suitable extenders are, for example, water, polar and nonpolar organic chemical liquids, for example from the classes of the aromatic and non-aromatic hydrocarbons (such as paraffins,

alkylbenzenes, alkylnaphthalenes, chlorobenzenes), the alcohols and polyols (which, if appropriate, may also be substituted, etherified and/or esterified), the ketones (such as acetone, cyclohexanone), esters

(including fats and oils) and (poly)ethers, the unsubstituted and substituted amines, amides, lactams (such as N-alkylpyrrolidones) and lactones, the sulphones and sulphoxides (such as dimethyl sulphoxide).

[0120] If the extender used is water, it is also possible to employ, for example, organic solvents as auxiliary solvents. Essentially, suitable liquid solvents are: aromatics such as xylene, toluene or alkylnaphthalenes, chlorinated aromatics and chlorinated aliphatic hydrocarbons such as chlorobenzenes, chloroethylenes or methylene chloride, aliphatic hydrocarbons such as cyclohexane or paraffins, for example petroleum fractions, mineral and vegetable oils, alcohols such as butanol or glycol and also their ethers and esters, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents such as dimethyl sulphoxide, and also water. [0121] Suitable solid carriers are for example, ammonium salts and ground natural minerals such as kaolins, clays, talc, chalk, quartz, attapulgite, montmorillonite or diatomaceous earth, and ground synthetic minerals, such as finely divided silica, alumina and silicates; suitable solid carriers for granules are: for example, crushed and fractionated natural rocks such as calcite, marble, pumice, sepiolite and dolomite, and also synthetic granules of inorganic and organic meals, and granules of organic material _{1 nr}

- 106 - such as paper, sawdust, coconut shells, maize cobs and tobacco stalks; suitable emulsifiers and/or foam- formers are: for example, nonionic and anionic emulsifiers, such as polyoxy ethylene fatty acid esters, polyoxyethylene fatty alcohol ethers, for example alkylaryl polyglycol ethers, alkylsulphonates, alkyl sulphates, arylsulphonates and also protein hydrolysates; suitable dispersants are nonionic and/or ionic substances, for example from the classes of the alcohol-POE and/or -POP ethers, acid and/or POP POE esters, alkylaryl and/or POP POE ethers, fat and/or POP POE adducts, POE- and/or POP-polyol derivatives, POE- and/or POP-sorbitan or -sugar adducts, alkyl or aryl sulphates, alkyl- or arylsulphonates and alkyl or aryl phosphates or the corresponding PO-ether adducts. Furthermore, suitable oligo- or polymers, for example those derived from vinylic monomers, from acrylic acid, from EO and/or PO alone or in combination with, for example, (poly)alcohols or (poly)amines. It is also possible to employ lignin and its sulphonic acid derivatives, unmodified and modified celluloses, aromatic and/or aliphatic sulphonic acids and their adducts with formaldehyde.

[0122] Tackifiers such as carboxymethylcellulose and natural and synthetic polymers in the form of powders, granules or latices, such as gum arabic, polyvinyl alcohol and polyvinyl acetate, as well as natural phospholipids such as cephalins and lecithins, and synthetic phospholipids, can be used in the formulations.

[0123] It is possible to use colorants such as inorganic pigments, for example iron oxide, titanium oxide and Prussian Blue, and organic dyestuffs, such as alizarin dyestuffs, azo dyestuffs and metal

phthalocyanine dyestuffs, and trace nutrients such as salts of iron, manganese, boron, copper, cobalt, molybdenum and zinc.

[0124] Other possible additives are perfumes, mineral or vegetable, optionally modified oils, waxes and nutrients (including trace nutrients), such as salts of iron, manganese, boron, copper, cobalt, molybdenum and zinc.

[0125] Stabilizers, such as low-temperature stabilizers, preservatives, antioxidants, light stabilizers or other agents which improve chemical and/or physical stability may also be present.

[0126] These individual types of formulation are known in principle and are described, for example, in: "Pesticides Formulations", 2nd Ed., Marcel Dekker N.Y.; Martens, 1979, "Spray Drying Handbook", 3rd Ed., G. Goodwin Ltd. London.

[0127] Based on his general expert knowledge, the person skilled in the art is able to choose suitable formulation auxiliaries (in this context, see, for example, Watkins, "Handbook of Insecticide Dust Diluents and Carriers", 2nd Ed., Darland Books, Caldwell N.J.).

[0128] In a preferred embodiment, the plants or plant parts are treated according to the invention with an oil-based suspension concentrate. An advantageous suspension concentrate is known from WO _n

- 107 -

2005/084435 (EP 1 725 104 A2). It consists of at least one room-temperature-solid active agrochemical substance, at least one "closed" penetrant, at least one vegetable oil or mineral oil, at least one nonionic surfactant and/or at least one anionic surfactant, and optionally one or more additives from the groups of the emulsifiers, foam inhibitors, preservatives, antioxidants, colorants and/or inert filler materials.

Preferred embodiments of the suspension concentrate are described in the above-mentioned WO

2005/084435. For the purpose of the disclosure, both documents are incorporated herein in their entirety by way of reference.

[0129] In a further preferred embodiment, the plants or plant parts are treated according to the invention with compositions comprising ammonium or phosphonium salts and, if appropriate, penetrants.

Advantageous compositions are known from WO2007/068355 and from the not prior-published EP 07109732.3. They consist of at least one compound of the formula (I) and at least one ammonium or phosphonium salt and, if appropriate, penetrants. Preferred embodiments are described in

WO2007/068355 and the not prior-published EP 07109732.3. For the purpose of the disclosure, these documents are incorporated herein in their entirety by way of reference. [0130] In general, the formulations comprise from 0.01 to 98% by weight of active compound, preferably from 0.5 to 90%>. In wettable powders, the active compound concentration is, for example, from about 10 to 90%> by weight, the remainder to 100%> by weight consisting of customary formulation components. In the case of emulsifiable concentrates, the active compound concentration can be from about 5 to 80%) by weight. In most cases, formulations in the form of dusts comprise from 5 to 20% by weight of active compound, sprayable solutions comprise about 2 to 20% by weight. In the case of granules, the active compound content depends partially on whether the active compound is present in liquid or solid form and on which granulation auxiliaries, fillers, etc., are used.

[0131] The required application rate may also vary with external conditions such as, inter alia, temperature and humidity. It may vary within wide limits, for example between 0.1 g/h and 5.0 kg/ha or more of active substance. However, they are preferably between 0.1 g/ha and 1.0 kg/ha. Owing to the synergistic effects between Bt vegetables and the insecticide, particular preference is given to application rates of from 0.1 to 500 g/ha.

[0132] For compounds of the formula (I), preference is given to application rates of from 10 to 500 g/ha; particularly preferred are from 10 to 200 g/ha. [0133] In a particular embodiment of the method according to the invention, the compound of the formula (I) is employed in an application rate of from 0.1 g/ha to 5.0 kg/ha, preferably from 0.1 to 500 g/ha and particularly preferably from 50 to 500 g/ha and especially preferably from 50 to 200 g/ha.

[0134] In their commercial formulations and in the use forms prepared from these formulations, the active compounds according to the invention may be present as mixtures with other active compounds, such as insecticides, attractants, sterilants, acaricides, nematicides, fungicides, growth-regulating substances or herbicides.

[0135] A mixture with other known compounds, such as herbicides, fertilizers, growth regulators, safeners, semiochemicals, or else with agents for improving plant properties is also possible. [0136] The active compound content of the use forms prepared from the commercial formulations can be from 0.00000001 to 95% by weight, preferably between 0.00001 and 1% by weight, of active compound.

Example

Compound (1-5) on transgenic Bt-plant

Spodoptera frugiperda - spray application on transgenic soy bean, field trial

[0137] For preparing the stock solution, 20 mg of active compound is solved in 200μ1 of

dimethylformamide and filled-up with 9.78 ml SC blank formulation of Belt. The final test concentrations are prepared by dilution with water.

[0138] The test is conducted with conventional soybean plants {Glycine max; non-transgenic) and transgenic soybean plants containing a Cryl Ac gene (Intacta from Monsanto). When the plants are in stage V2 (3 nodes with 2 unfolded trifoliolates), they are treated by spray application with the active compound preparation. After application, clip-cages with 5-6 L2 larvae of the fall army worm

{Spodoptera frugiperda) are placed on the leaves.

[0139] After the specified period of time, feeding damage (white holes on leaves) of Spodoptera frugiperda on conventional soybean, Fig. la, in comparison to Intacta soybean, Fig. lb, is visualized on 3 randomly picked soybean leaves out of 5 replicate plots (R1-R5). [0140] According to the present application in this test the following combinations of transgenic plant and compound shows a superior effect compared to the treated, non-transgenic plant respectively the non- treated, transgenic plant:

[0141] Table A

Results of the experiments 1, 2 and 9 to 18 of Table A are shown in Fig la and lb

Claims

claims:

Method for improving the utilization of the production potential of a transgenic plant and/or for controlling/combating/treating insect or nematode pests, characterized in that the plant is treated with an effective amount of at least one compound of the formula (I)

wherein

A represents individually halogen, cyano, nitro, hydroxyl, amino, Ci-Cs alkyl group, substituted Ci-Cs alkyl group having at least one substituent elected from the group consisting of halogen, hydroxy, cyano, nitro, amino, halo C1-C3 alkyl group, C1-C3 alkoxy group, halo C1-C3 alkoxy group, C1-C3 alkylthio group, halo C1-C3 alkylthio group, C1-C3 alkylsulfinyl group, halo C1-C3 alkylsulfinyl group, C1-C3 alkylsulfonyl group, halo C1-C3 alkylsulfonyl group and C1-C3 alkylthio, C1-C3 alkyl group; further, an arbitrary saturated carbon atom in said optionally substituted Ci-Cs alkyl group; n represents 0, 1, 2, 3 or 4, preferably 0, 1 or 2;

Ri represents hydrogen, halogen, cyano Ci-Cs alkyl or Ci-Cs haloalkyl;

R2 represents hydrogen, halogen, cyano Ci-Cs alkyl or Ci-Cs haloalkyl;

R3 represents O or S;

R represents O or S;

Y represents individually hydrogen, halogen, cyano, nitro, Ci-Ce alkyl group, halo Ci-Ce alkyl group, C2-C6 alkenyl group, halo C2-C6 alkenyl group, C2-C6 alkynyl group, halo C2-C6 alkynyl group, C3-C6 cycloalkyl group, halo C3-C6 cycloalkyl group, Ci-Ce alkoxy group, halo Ci-Ce alkoxy group, Ci-Ce alkylthio group, halo Ci-Ce alkylthio group, Ci-Ce alkylsulfinyl group, halo Ci-Ce alkylsulfinyl group, Ci-Ce alkylsulfonyl group, or halo Ci-Ce alkylsulfonyl group;

m represents 0, 1, 2, 3, or 4;

X represents a Ci-Cs alkyl group or a substituted Ci-Cs alkyl group having at least one substituent selected from the group consisting of halogen, hydroxy, cyano, nitro, amino, halo C1-C3 alkyl group, C1-C3 alkoxy group, halo C1-C3 alkoxy group - I l l -

Method according to Claim 1 , characterized in that the compound of the formula (I) is formula (I-

wherein

Hal represents F, CI, I or Br; and

X' represents Ci-Ce alkyl or substituted Ci-Ce alkyl having at least one substituent selected from the group consisting of halogen, hydroxy, cyano, nitro, amino, halo C1-C3 alkyl group, preferably a Ci-C6 cyanoalkyl;

A' represents C1-C3 alkyl, C1-C3 haloalkyl, halogen, preferably methyl, halomethyl, ethyl or haloethyl, more preferably methyl or ethyl; n represents 0, 1,

2,

3 or 4, preferably 0, 1 or 2, more preferably 1.

Method according to Claim 1 or Claim 2, characterized in that the compound of the formula (I) is selected from the group consisting of compound (1-2), (1-3), (1-4) or (1-5):

4. Method according to Claim 3, characterized in that the compound of the formula (I) is compound

5. Method according to any of Claims 1 to 4, characterized in that the transgenic plant contains at least one cry-gene or a cry-gene fragment coding for a Bt toxin.

6. Method according to Claim 5, characterized in that the Bt toxin is encoded by a cry gene or a cry- gene fragment selected from the subgroup crylA.

7. Method according to claim 6, characterized in that the Bt toxin is encoded by a cry gene or a cry- gene fragment selected from the subgroups cryl Aa, cryl Ab and cryl Ac or a hybrid thereof.

8. Method according to any one of claims 1 to 8, characterized in that the Bt toxin is encoded by a bt-gene or fragmetn thereof comprising event MON87701.

9. Method according to any one of claims 1 to 8, characterized in that the transgenic plant is a vegetable plant, maize plant, soya bean plant, cotton plant, tobacco plant, rice plant, sugar beet plant, oilseed rape plant or potato plant.

10. Method according to any of Claims 1 to 9, characterized in that the use form of the compound of the formula (I) is present in a mixture with at least one mixing partner.

11. Synergistic composition comprising a Bt toxin, preferably a Bt toxin encoded by a bt-gene or fragment thereof comprising event MON87701, and a compound of formula (I) as described in any one of claims 1 to 4. - 113 -

12. Synergistic composition according to claim 11, characterized in that the Bt toxin is encoded by a cry gene or a cry-gene fragment selected from the group consisting of cryl, cry2, cry3, cry5 and cry9, preferably cryl.

13. Synergistic composition according to claim 12, characterized in that the Bt toxin is encoded by a cry gene or a cry-gene fragment selected from the subgroup crylA, especially preferred crylAa, crylAb and crylAc.

14. Synergistic composition according to claim 13, characterized in that the Bt toxin is encoded by a bt-gene or fragment thereof comprising event MON87701.

15. A Bt plant, charcterized in that at least 0.00001 g of a compound of formula (I), preferrably compound (1-5), is attached to it.