WO2014147191A1 - Biocapteurs peptidiques fluorescents à auto-pénétration de cellule pour sonder et quantifier les cdk/cycline kinases - Google Patents

Biocapteurs peptidiques fluorescents à auto-pénétration de cellule pour sonder et quantifier les cdk/cycline kinases Download PDF

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Publication number
WO2014147191A1
WO2014147191A1 PCT/EP2014/055631 EP2014055631W WO2014147191A1 WO 2014147191 A1 WO2014147191 A1 WO 2014147191A1 EP 2014055631 W EP2014055631 W EP 2014055631W WO 2014147191 A1 WO2014147191 A1 WO 2014147191A1
Authority
WO
WIPO (PCT)
Prior art keywords
cdk
cyclin
compound
amino acid
sample
Prior art date
Application number
PCT/EP2014/055631
Other languages
English (en)
Inventor
May Catherine MORRIS
Laeticia KURZAWA
Morgan PELLERANO
Original Assignee
Centre National De La Recherche Scientifique (Cnrs)
Universite Montpellier 2 Sciences Et Techniques
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National De La Recherche Scientifique (Cnrs), Universite Montpellier 2 Sciences Et Techniques filed Critical Centre National De La Recherche Scientifique (Cnrs)
Publication of WO2014147191A1 publication Critical patent/WO2014147191A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4739—Cyclin; Prad 1
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Abstract

La présente invention concerne des composés comprenant un polypeptide et un fluorophore, lesdits composés étant capables de pénétrer directement dans des cellules vivantes et de se lier aux complexes CDK/cycline. Par conséquent, les composés selon l'invention sont capables de sonder l'abondance relative de ces complexes protéiques. La présente invention concerne en outre un procédé pour déterminer la présence de complexes CDK/cycline dans des échantillons de cellules par l'intermédiaire d'une stratégie de quantification quotientométrique au moyen de composés selon l'invention. L'invention concerne finalement l'utilisation desdits composés et/ou de compositions comprenant lesdits composés pour la détection in vitro de la présence et/ou du taux d'expression de complexes CDK/cycline, pour le criblage à rendement élevé et en grand volume de banques de composés et pour l'imagerie médicale.
PCT/EP2014/055631 2013-03-20 2014-03-20 Biocapteurs peptidiques fluorescents à auto-pénétration de cellule pour sonder et quantifier les cdk/cycline kinases WO2014147191A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13305338.9 2013-03-20
EP13305338 2013-03-20

Publications (1)

Publication Number Publication Date
WO2014147191A1 true WO2014147191A1 (fr) 2014-09-25

Family

ID=48044709

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2014/055631 WO2014147191A1 (fr) 2013-03-20 2014-03-20 Biocapteurs peptidiques fluorescents à auto-pénétration de cellule pour sonder et quantifier les cdk/cycline kinases

Country Status (1)

Country Link
WO (1) WO2014147191A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019043946A (ja) * 2017-08-31 2019-03-22 国立大学法人埼玉大学 分子精製用リガンド、分子精製用タグペプチド及びこれらを用いた分子精製方法

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
C. GONDEAU: "Design of a Novel Class of Peptide Inhibitors of Cyclin-dependent Kinase/Cyclin Activation", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 280, no. 14, 1 January 2005 (2005-01-01), pages 13793 - 13800, XP055057298, ISSN: 0021-9258, DOI: 10.1074/jbc.M413690200 *
DUK-SOO KIM ET AL: "PEP-1-p18 prevents neuronal cell death by inhibiting oxidative stress and Bax expression", BMB REPORTS, vol. 45, no. 9, 30 September 2012 (2012-09-30), pages 532 - 537, XP055076817, ISSN: 1976-6696, DOI: 10.5483/BMBRep.2012.45.9.083 *
HEITZ FREDERIC ET AL: "Twenty years of cell-penetrating peptides: from molecular mechanisms to therapeutics", BRITISH JOURNAL OF PHARMACOLOGY, NATURE PUBLISHING GROUP, BASINGSTOKE, HANTS; GB, vol. 157, no. 2, 1 May 2009 (2009-05-01), pages 195 - 206, XP008118202, ISSN: 0007-1188, [retrieved on 20090320], DOI: 10.1111/J.1476-5381.2009.00057.X *
KURZAWA L ET AL: "Fluorescent peptide biosensor for probing the relative abundance of cyclin-dependent kinases in living cells", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 6, 1 January 2011 (2011-01-01), pages e26555,1 - 11, XP009168416, ISSN: 1932-6203, [retrieved on 20111018], DOI: 10.1371/JOURNAL.PONE.0026555 *
KURZAWA L ET AL: "PEP and CADY-mediated delivery of fluorescent peptides and proteins into living cells", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - BIOMEMBRANES, ELSEVIER, AMSTERDAM, NL, vol. 1798, no. 12, 1 December 2010 (2010-12-01), pages 2274 - 2285, XP027430150, ISSN: 0005-2736, [retrieved on 20100225], DOI: 10.1016/J.BBAMEM.2010.02.027 *
NHU NGOC VAN THI ET AL: "Fluorescent sensors of protein kinases: from basics to biomedical applications", PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, ACADEMIC PRESS, US, vol. 113, 13 December 2012 (2012-12-13), pages 217 - 274, XP009168168, ISSN: 0079-6603, [retrieved on 20130320], DOI: 10.1016/B978-0-12-386932-6.00006-5 *
SHIRONG YAN ET AL: "Study on the penetrability of PEP-1-P27mt for cell membranes in Vitro", JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY, vol. 27, no. 3, 1 June 2007 (2007-06-01), pages 225 - 229, XP055076672, ISSN: 1672-0733, DOI: 10.1007/s11596-007-0302-2 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019043946A (ja) * 2017-08-31 2019-03-22 国立大学法人埼玉大学 分子精製用リガンド、分子精製用タグペプチド及びこれらを用いた分子精製方法

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