WO2014139260A1 - System and method for particle manipulation and sorting based on structural acoustic field - Google Patents

System and method for particle manipulation and sorting based on structural acoustic field Download PDF

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Publication number
WO2014139260A1
WO2014139260A1 PCT/CN2013/081208 CN2013081208W WO2014139260A1 WO 2014139260 A1 WO2014139260 A1 WO 2014139260A1 CN 2013081208 W CN2013081208 W CN 2013081208W WO 2014139260 A1 WO2014139260 A1 WO 2014139260A1
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WIPO (PCT)
Prior art keywords
particles
artificial structure
acoustic
manipulation
signal generator
Prior art date
Application number
PCT/CN2013/081208
Other languages
French (fr)
Inventor
Hairong Zheng
Feiyan CAI
Fei Li
Long Meng
Chen Wang
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Shenzhen Institutes Of Advanced Technology
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Publication of WO2014139260A1 publication Critical patent/WO2014139260A1/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
    • B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
    • B07C5/34—Sorting according to other particular properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
    • B07C2501/00—Sorting according to a characteristic or feature of the articles or material to be sorted
    • B07C2501/0027—Sorting the articles according to a sound

Definitions

  • the present invention relates to acoustic manipulation technology, particularly relates to a system and method for particle manipulation and sorting based on structural acoustic field.
  • micro-manipulation technology influences biology, quantum optics, soft matter physics, biophysics and clinical medicine significantly.
  • the micro-manipulation technology not only provides important research means to investigate fundamental mechanics, physics and biochemistry characteristics of micro/nano particles such as metal, cell and DNA, but also provides necessary technical support for development of novel biochemistry analysis instruments used for biological particles sorting such as cell, blood platelet. To develop precise, reliable and low cost manipulation devices for micro/nano particles is becoming one of the highly anticipated front direction.
  • the current manipulation technology for micro/nano particles could be classified as: manipulation technology based on micro forceps and micropipette; manipulation technology based on surface force, such as surface tension, adhesion, electrostatic force; manipulation technology based on external force field, such as optical, sound and magnetic field.
  • manipulation technology based on external force field has an advantage of no-contact and becomes the focus of a wide and rapidly expanding field of research.
  • the methods using optical field, magnetic field, electrophoresis, fluid dynamics realize the manipulation of particles, the disadvantages of the noncontact manipulation methods are difficult to miniaturize, easy to damage bio-particles, and necessary to modify the surface of the desired micro/nano -particles.
  • acoustic manipulation technology has been paid attention to extensively due to its contactless, non-invasive and universally applicable advantage.
  • Current acoustic manipulation mainly uses the focused sound field and standing- wave acoustic field to manipulate micro/nano particles.
  • the prior art uses fluid or standing -wave acoustic field to align the micro/nano particles into a line one by one first, then the aligned particles are transported to the next acoustic field, where the particles are divided into different groups according to the sizes or acoustic characteristics while flowing through the acoustic field, thus the sorting could be realized.
  • the prior art either processes single particle or the particle flowing through the acoustic field individually, which determines that this kind of sorting technology could not do batch processing of micro/nano particles, and could not do parallel processing of batch particles.
  • the technical problem to be solved by the present application is to provide a system and method for a group of randomly mixed particles manipulation and sorting based on structural acoustic field against the drawbacks of prior art.
  • the present application provides a system for particle manipulation and sorting based on structural acoustic field, comprising sample holder, ultrasound transmission device and artificial structure, the sample holder is used to place particles to be sorted, the ultrasound transmission device is used to transmit ultrasound wave, the artificial structure is periodic structure used to modulate acoustic field to produce stronger acoustic radiation force and sort the particles on the sample holder.
  • the periodic structural includes substrate and a plurality of raised lines in parallel placement on the substrate with equal interval.
  • the raised lines are cuboids, polygonal prisms or semi-cylinders.
  • the spacing between the central lines of each two adjacent cuboids is d
  • the thickness of the substrate is h 2
  • the artificial structure uses rigid materials with shear wave velocity more than longitudinal wave band of water used as the substrate.
  • the ultrasound transmission device includes signal generator, power amplifier and ultrasonic transducer
  • the signal generator is used to produce transmission signal
  • the transmission signal excites the ultrasonic transducer via the power amplifier to produce ultrasound wave.
  • the signal generator is also used to set the transmission frequency to be the resonance frequency of the artificial structure, realizing acoustic manipulation through voltage adjustment; or the signal generator is used to set the excitation voltage of the signal generator to be a fixed value, realizing acoustic manipulation through frequency adjustment.
  • the system for particle manipulation and sorting based on structural acoustic field further includes video module and image analysis module, the video module is used to record images of the release of the captured particles, the image analysis module is used to measure the released particles.
  • the present application provides a method for particle manipulation and sorting based on structural acoustic field using the above-mentioned artificial structure, comprising: Placing particles to be sorted on sample holder, placing the artificial structure above the sample holder;
  • Ultrasound transmission device transmitting ultrasound wave
  • the artificial structure modulating the incident acoustic field
  • the artificial structure sorting the particles on the sample holder based on the acoustic radiation force produced by the modulation.
  • the artificial structure tuning the acoustic field further comprising:
  • the method for particle manipulation and sorting based on structural acoustic field further comprising:
  • the artificial structure being periodic structure, the particles being sorted, plurality of particles could be placed on the sample holder simultaneously, the artificial structure is used to modulate the acoustic field to produce stronger acoustic radiation force, capturing the specific particles under the lower surface of the artificial structure at the same time, realizing rapid, batch sorting and increasing the efficiency.
  • the transmission frequency of the signal generator being set to be the resonance frequency of the artificial structure, realizing the acoustic manipulation through voltage adjustment; or the excitation voltage of the signal generator being set to be a fixed value, realizing the acoustic manipulation through frequency adjustment, because there are certain, stable relationship between the acoustic pressure or frequency and the micro/nano particles, under specific acoustic pressure or frequency, the artificial structure could capture either the particles of specific sizes with the same mass density or the particles of different mass density with the same size distribution, enabling the sorting result to be reproducible and not to be disturbed by external factors, resulting in higher reliability.
  • Fig. 1 is schematic diagram according to one embodiment of the present application of the system for particle manipulation and sorting based on structural acoustic field;
  • Fig. 2 is schematic diagram according to another embodiment of the present application of the system for particle manipulation and sorting based on structural acoustic field;
  • Fig. 3 is schematic diagram of the artificial structure according to one embodiment of the present application of the system for particle manipulation and sorting based on structural acoustic field;
  • Fig. 4 is flowchart according to one embodiment of the present application of the method for particle manipulation and sorting based on structural sound field
  • Fig. 5 is sorting effects diagram of two kinds of glass beads being sorted according to the present application.
  • Fig. 6 is sorting effects diagram of three kinds of glass beads being sorted according to the present application.
  • Embodiment 1 is a diagrammatic representation of Embodiment 1 :
  • the system for particle manipulation and sorting based on structural acoustic field comprises sample holder, ultrasound transmission device and artificial structure, the sample holder is used to place particles to be sorted; the ultrasound transmission device used to transmit ultrasound wave, the artificial structure is periodic structure and modulates the acoustic field to produce stronger acoustic radiation forces and sorts the particles to be sorted.
  • the sample holder is acoustic transmission phantom made of gel, the height is adjustable if needed. In this embodiment, the height of the sample holder is 4cm.
  • the ultrasound transmission device includes signal generator, power amplifier and ultrasonic transducer, the signal generator is used to produce transmission signal, which could excite the ultrasonic transducer through the power amplifier to produce ultrasound wave.
  • the ultrasonic transducer could be one of single element ultrasonic transducer, phased array ultrasonic transducer, linear array ultrasonic transducer, convex array ultrasonic transducer or interdigital transducer. The sizes of the particles to be sorted determine parameters of the artificial structure, thus determine the resonance frequency of the artificial structure.
  • the ultrasonic transducer uses single element ultrasonic transducer with the center frequency of 1.22MHz and focal distance of 120mm.
  • the transmission signal of the signal generator could be continuous sinusoidal signal or pulsar sinusoidal signal.
  • the signal generator could be programmable signal generator(AFG3021,Tectronix), the power amplifier could be 50db linear power amplifier (325LA, ENI).
  • the signal generator produces continuous sinusoidal signals which excite the ultrasonic transducer through the power amplifier to produce ultrasound wave.
  • the video module is used to video the images of released particles after capture, the image analysis module is used to measure the released particles.
  • the video module includes continuous lens (XDS-N0745i), industrial camera CCD (DL-300), microscope stand (DT- 100) and video software provided by the CCD vender.
  • the image analysis module is Image J, Java image processing program of public domain.
  • the system for particle manipulation and sorting based on structural acoustic field further includes 3D displacement module, used to control the spatial location of the artificial structure and the ultrasound transmission device.
  • the 3D displacement module includes precision electrically moving table (KSA, ZOLIX) and electrically moving table control cabinet (MC600, ZOLIX).
  • the periodic structure could include substrate 11 and a plurality of raised lines 12, the plurality of raised lines 12 are in parallel placement on the substrate with equal interval between each two adjacent raised lines 12.
  • the artificial structure according to one embodiment of the present application is a fence structure.
  • the raised lines 12 could be cuboids, polygonal prisms or semi-cylinders, also could be formed to other shapes.
  • the raised lines are cuboids, assuming the spacing between the central lines of each two adjacent cuboids is d, the thickness of the substrate is h 2 , then 0.15 ⁇ h 2 /d ⁇ 0.25.
  • the spacing between the central lines of each two adjacent cuboids, d is the period of the artificial structure, the artificial structure is made from hard material, specifically made from the hard material with shear wave velocity more than longitudinal wave band of water used as the substrate.
  • the hard material could be metal, such as copper, aluminum, steel or other metal material, or non-metallic material.
  • the artificial structure is fence structure with the period of 0.82mm, height of 0.15mm and width of 0.15mm made from copper plate with thickness of 0.3mm.
  • Embodiment 2 is a diagrammatic representation of Embodiment 1:
  • one embodiment of the method for particle manipulation and sorting based on structural acoustic field comprises the following steps:
  • Step402 Placing particles to be sorted on sample holder, placing the artificial structure above the sample holder; in this embodiment, the particles to be sorted could be glass beads, specifically to be pickling glass beads, G4649- 100G,G1145-100G and G8772-100G produced by Sigma-Aldrich.
  • the diameters of these three types of glass beads are ⁇ 106 ⁇ , 150-212 ⁇ ,425-600 ⁇ respectively.
  • Step404 Ultrasound transmission device transmitting ultrasound wave
  • Step406 The artificial structure modulating the acoustic field
  • Step408 The artificial structure sorting the particles to be sorted based on the acoustic radiation force produced by the modulation.
  • the artificial structure could trap the sorted particles on the lower surface of the artificial structure.
  • the step406 further comprises:
  • the transmission frequency of the signal generator is set to be the resonance frequency of the artificial structure, thus realizing the acoustic manipulation through voltage adjustment;
  • the signal generator produces continuous sinusoidal signals with the frequency of 1.282MHz, the voltage could be raised to 1500mVpp from lOOmVpp, or decreased to lOOmVpp from 1500mVpp, with the step of lmVpp.
  • the sinusoidal signals could excite the ultrasonic transducer through the power amplifier to generate ultrasound wave; the ultrasound excites the artificial structure to vibrate, thus the trapping forces could be produced on the surface of the artificial structure.
  • the step406 specifically comprises:
  • the excitation voltage is set to be a fixed value, the ultrasound could be modulated through frequency adjustment.
  • the signal generator produces continuous sinusoidal signals with the voltage of 1500mVpp, the frequency could be increased to 1.282MHz from 1.171MHz, or decreased to 1.171MHz from 1.282MHz, with the step of O.OOlMHz.
  • the sinusoidal signals could excite the ultrasonic transducer through the power amplifier to generate ultrasound wave; the ultrasound excites the artificial structure to vibrate, thus the trapping forces could be produced on the surface of the artificial structure.
  • One embodiment of the method for particle manipulation and sorting based on structural acoustic field according to the present application further comprises the following steps:
  • Step410 Releasing captured particles, and videoing the released particles
  • Step412 Measuring the sizes and numbers of the released particles. Whether the diameters of the particles meeting the requirements or not, whether the sorting goal being achieved or not could be inspected with the measurement.
  • Fig. 5 illustrates the trapping and sorting results of the mixed particles of the two types of glass beads with the diameters of 150-212 ⁇ and 425-600 ⁇ .
  • the signal generator produces continuous sinusoidal signals with the frequency of 1.282MHz, which excites the ultrasonic transducer through the power amplifier to produce ultrasound wave, the ultrasound excites the artificial structure to vibrate, thus the trapping forces could be produced on the surface of the artificial structure.
  • Fig. 5(a) in case of voltage of 1500mVpp, the surface of the artificial structure videoed by the microscope video module captures two sizes of the glass beads.
  • Fig. 5 in case of voltage of 1500mVpp, the surface of the artificial structure videoed by the microscope video module captures two sizes of the glass beads.
  • Fig.6 illustrates the trapping and sorting result of three types of glass beads with diameters of ⁇ 106 ⁇ , 150-212 ⁇ and 425-600 ⁇ .
  • the signal generator produces continuous sinusoidal signals with the frequency of
  • the surface of the artificial structure videoed by the microscope video module captures the periodically arrayed glass beads with the diameter of ⁇ 106 ⁇ ;
  • the glass beads of 150-212 ⁇ are trapped onto the surface of the artificial structure and arrayed periodically;
  • the glass beads of 425-600 ⁇ are also trapped onto the surface of the artificial structure, at that time, it can be seen that three sizes of the glass beads are captured onto the surface of the artificial structure.

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  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

A system for particle manipulation and sorting based on structural acoustic field comprises a sample holder, an ultrasound transmission device and an artificial structure, the sample holder is used to place particles to be sorted, the ultrasound transmission device is used to transmit ultrasound wave, the artificial structure is periodic structure used to modulate acoustic field to produce stronger acoustic radiation force and sort the particles to be sorted. And there is a method for particle manipulation and sorting based on structural acoustic field. Due to the sample holder, the ultrasound transmission device and the artificial structure, plurality of particles could be placed on the sample holder simultaneously, the artificial structure is used to modulate the acoustic field to produce stronger acoustic radiation force, trapping the specific particles under the lower surface of the artificial structure at the same time, realizing rapid, batch sorting and increasing the efficiency.

Description

System and Method for Particle Manipulation and Sorting Based on
Structural Acoustic Field
FIELD OF THE INVENTION
The present invention relates to acoustic manipulation technology, particularly relates to a system and method for particle manipulation and sorting based on structural acoustic field.
BACKGROUND OF THE INVENTION
Research and development on micro -manipulation technology of single atom or macroscopic material influences biology, quantum optics, soft matter physics, biophysics and clinical medicine significantly. The micro-manipulation technology not only provides important research means to investigate fundamental mechanics, physics and biochemistry characteristics of micro/nano particles such as metal, cell and DNA, but also provides necessary technical support for development of novel biochemistry analysis instruments used for biological particles sorting such as cell, blood platelet. To develop precise, reliable and low cost manipulation devices for micro/nano particles is becoming one of the highly anticipated front direction.
The current manipulation technology for micro/nano particles could be classified as: manipulation technology based on micro forceps and micropipette; manipulation technology based on surface force, such as surface tension, adhesion, electrostatic force; manipulation technology based on external force field, such as optical, sound and magnetic field. Wherein the manipulation technology based on external force field has an advantage of no-contact and becomes the focus of a wide and rapidly expanding field of research. Currently, although the methods using optical field, magnetic field, electrophoresis, fluid dynamics realize the manipulation of particles, the disadvantages of the noncontact manipulation methods are difficult to miniaturize, easy to damage bio-particles, and necessary to modify the surface of the desired micro/nano -particles. Acoustic manipulation technology has been paid attention to extensively due to its contactless, non-invasive and universally applicable advantage. Current acoustic manipulation mainly uses the focused sound field and standing- wave acoustic field to manipulate micro/nano particles. The prior art uses fluid or standing -wave acoustic field to align the micro/nano particles into a line one by one first, then the aligned particles are transported to the next acoustic field, where the particles are divided into different groups according to the sizes or acoustic characteristics while flowing through the acoustic field, thus the sorting could be realized. The prior art either processes single particle or the particle flowing through the acoustic field individually, which determines that this kind of sorting technology could not do batch processing of micro/nano particles, and could not do parallel processing of batch particles.
SUMMARY OF THE INVENTION
The technical problem to be solved by the present application is to provide a system and method for a group of randomly mixed particles manipulation and sorting based on structural acoustic field against the drawbacks of prior art.
According to first aspect, the present application provides a system for particle manipulation and sorting based on structural acoustic field, comprising sample holder, ultrasound transmission device and artificial structure, the sample holder is used to place particles to be sorted, the ultrasound transmission device is used to transmit ultrasound wave, the artificial structure is periodic structure used to modulate acoustic field to produce stronger acoustic radiation force and sort the particles on the sample holder.
In the system for particle manipulation and sorting based on structural acoustic field, wherein the periodic structural includes substrate and a plurality of raised lines in parallel placement on the substrate with equal interval.
In the system for particle manipulation and sorting based on structural acoustic field, wherein the raised lines are cuboids, polygonal prisms or semi-cylinders.
In the system for particle manipulation and sorting based on structural acoustic field, wherein the raised lines are cuboids, the spacing between the central lines of each two adjacent cuboids is d, the thickness of the substrate is h2, then 0.15<h2/d<0.25.
In the system for particle manipulation and sorting based on structural acoustic field, wherein the width, height of the cuboid and the thickness of the substrate are equal.
In the system for particle manipulation and sorting based on structural acoustic field, wherein the artificial structure uses rigid materials with shear wave velocity more than longitudinal wave band of water used as the substrate.
In the system for particle manipulation and sorting based on structural acoustic field, wherein the ultrasound transmission device includes signal generator, power amplifier and ultrasonic transducer, the signal generator is used to produce transmission signal, the transmission signal excites the ultrasonic transducer via the power amplifier to produce ultrasound wave.
In the system for particle manipulation and sorting based on structural acoustic field, wherein the signal generator is also used to set the transmission frequency to be the resonance frequency of the artificial structure, realizing acoustic manipulation through voltage adjustment; or the signal generator is used to set the excitation voltage of the signal generator to be a fixed value, realizing acoustic manipulation through frequency adjustment.
The system for particle manipulation and sorting based on structural acoustic field further includes video module and image analysis module, the video module is used to record images of the release of the captured particles, the image analysis module is used to measure the released particles.
According to second aspect of the present application, the present application provides a method for particle manipulation and sorting based on structural acoustic field using the above-mentioned artificial structure, comprising: Placing particles to be sorted on sample holder, placing the artificial structure above the sample holder;
Ultrasound transmission device transmitting ultrasound wave;
The artificial structure modulating the incident acoustic field;
The artificial structure sorting the particles on the sample holder based on the acoustic radiation force produced by the modulation.
In the method for particle manipulation and sorting based on structural acoustic field, wherein the artificial structure tuning the acoustic field, further comprising:
Setting the transmission frequency of the signal generator to be the resonance frequency of the artificial structure, realizing the acoustic manipulation through voltage adjustment.
Or setting the excitation voltage of the signal generator to be a fixed value, realizing the acoustic manipulation through frequency adjustment.
The method for particle manipulation and sorting based on structural acoustic field, further comprising:
Releasing the captured particles and videoing the released particles;
Measuring the released particles.
Due to the above technical solutions used, the advantageous effects produced by the present application are:
(1) In embodiments of the present application, due to the sample holder, ultrasound transmission device and artificial structure, and the ultrasound transmission device used to transmit ultrasound, the artificial structure being periodic structure, the particles being sorted, plurality of particles could be placed on the sample holder simultaneously, the artificial structure is used to modulate the acoustic field to produce stronger acoustic radiation force, capturing the specific particles under the lower surface of the artificial structure at the same time, realizing rapid, batch sorting and increasing the efficiency.
(2) In embodiments of the present application, due to the transmission frequency of the signal generator being set to be the resonance frequency of the artificial structure, realizing the acoustic manipulation through voltage adjustment; or the excitation voltage of the signal generator being set to be a fixed value, realizing the acoustic manipulation through frequency adjustment, because there are certain, stable relationship between the acoustic pressure or frequency and the micro/nano particles, under specific acoustic pressure or frequency, the artificial structure could capture either the particles of specific sizes with the same mass density or the particles of different mass density with the same size distribution, enabling the sorting result to be reproducible and not to be disturbed by external factors, resulting in higher reliability.
BRIEF DESCRIPTION OF THE DRAWINGS
Fig. 1 is schematic diagram according to one embodiment of the present application of the system for particle manipulation and sorting based on structural acoustic field;
Fig. 2 is schematic diagram according to another embodiment of the present application of the system for particle manipulation and sorting based on structural acoustic field;
Fig. 3 is schematic diagram of the artificial structure according to one embodiment of the present application of the system for particle manipulation and sorting based on structural acoustic field;
Fig. 4 is flowchart according to one embodiment of the present application of the method for particle manipulation and sorting based on structural sound field
Fig. 5 is sorting effects diagram of two kinds of glass beads being sorted according to the present application;
Fig. 6 is sorting effects diagram of three kinds of glass beads being sorted according to the present application.
DETAILED DESCRIPTION OF THE INVENTION
Hereafter is the detailed description to the present application with embodiments in conjunction with the drawings.
Embodiment 1 :
As shown in Fig. 1 -3, the system for particle manipulation and sorting based on structural acoustic field according to one embodiment of the present application, comprises sample holder, ultrasound transmission device and artificial structure, the sample holder is used to place particles to be sorted; the ultrasound transmission device used to transmit ultrasound wave, the artificial structure is periodic structure and modulates the acoustic field to produce stronger acoustic radiation forces and sorts the particles to be sorted.
In one embodiment, the sample holder is acoustic transmission phantom made of gel, the height is adjustable if needed. In this embodiment, the height of the sample holder is 4cm.
In one embodiment, the ultrasound transmission device includes signal generator, power amplifier and ultrasonic transducer, the signal generator is used to produce transmission signal, which could excite the ultrasonic transducer through the power amplifier to produce ultrasound wave. The ultrasonic transducer could be one of single element ultrasonic transducer, phased array ultrasonic transducer, linear array ultrasonic transducer, convex array ultrasonic transducer or interdigital transducer. The sizes of the particles to be sorted determine parameters of the artificial structure, thus determine the resonance frequency of the artificial structure. In this embodiment, the ultrasonic transducer uses single element ultrasonic transducer with the center frequency of 1.22MHz and focal distance of 120mm. The transmission signal of the signal generator could be continuous sinusoidal signal or pulsar sinusoidal signal. In one embodiment, the signal generator could be programmable signal generator(AFG3021,Tectronix), the power amplifier could be 50db linear power amplifier (325LA, ENI). The signal generator produces continuous sinusoidal signals which excite the ultrasonic transducer through the power amplifier to produce ultrasound wave.
The video module is used to video the images of released particles after capture, the image analysis module is used to measure the released particles. In one embodiment, the video module includes continuous lens (XDS-N0745i), industrial camera CCD (DL-300), microscope stand (DT- 100) and video software provided by the CCD vender. The image analysis module is Image J, Java image processing program of public domain.
The system for particle manipulation and sorting based on structural acoustic field according to the present application further includes 3D displacement module, used to control the spatial location of the artificial structure and the ultrasound transmission device. In one embodiment, the 3D displacement module includes precision electrically moving table (KSA, ZOLIX) and electrically moving table control cabinet (MC600, ZOLIX).
In one embodiment, the periodic structure could include substrate 11 and a plurality of raised lines 12, the plurality of raised lines 12 are in parallel placement on the substrate with equal interval between each two adjacent raised lines 12. The artificial structure according to one embodiment of the present application is a fence structure.
The raised lines 12 could be cuboids, polygonal prisms or semi-cylinders, also could be formed to other shapes. In this embodiment, the raised lines are cuboids, assuming the spacing between the central lines of each two adjacent cuboids is d, the thickness of the substrate is h2, then 0.15<h2/d<0.25. The spacing between the central lines of each two adjacent cuboids, d, is the period of the artificial structure, the artificial structure is made from hard material, specifically made from the hard material with shear wave velocity more than longitudinal wave band of water used as the substrate. The hard material could be metal, such as copper, aluminum, steel or other metal material, or non-metallic material. In this embodiment, the artificial structure is fence structure with the period of 0.82mm, height of 0.15mm and width of 0.15mm made from copper plate with thickness of 0.3mm.
In one embodiment, the width, height of the cuboid and the thickness of the substrate could be equal, that is, assuming the width of the cuboid is w, the height of the cuboid is hi, then w=h!=h2. In this embodiment, the artificial structure is fence structure with the period of 0.82mm, height of 0.15mm and width of 0.15mm made by laser etching on copper plate with thickness of h!+h2=0.3mm.
Embodiment 2:
As shown in Fig. 4, one embodiment of the method for particle manipulation and sorting based on structural acoustic field according to the present application, comprises the following steps:
Step402: Placing particles to be sorted on sample holder, placing the artificial structure above the sample holder; in this embodiment, the particles to be sorted could be glass beads, specifically to be pickling glass beads, G4649- 100G,G1145-100G and G8772-100G produced by Sigma-Aldrich. The diameters of these three types of glass beads are <106μιη, 150-212μιη,425-600μιη respectively.
Step404:Ultrasound transmission device transmitting ultrasound wave;
Step406:The artificial structure modulating the acoustic field;
Step408: The artificial structure sorting the particles to be sorted based on the acoustic radiation force produced by the modulation. The artificial structure could trap the sorted particles on the lower surface of the artificial structure.
In one embodiment of the method for particle manipulation and sorting based on structural acoustic field according to the present application, the step406 further comprises:
The transmission frequency of the signal generator is set to be the resonance frequency of the artificial structure, thus realizing the acoustic manipulation through voltage adjustment; the signal generator produces continuous sinusoidal signals with the frequency of 1.282MHz, the voltage could be raised to 1500mVpp from lOOmVpp, or decreased to lOOmVpp from 1500mVpp, with the step of lmVpp. The sinusoidal signals could excite the ultrasonic transducer through the power amplifier to generate ultrasound wave; the ultrasound excites the artificial structure to vibrate, thus the trapping forces could be produced on the surface of the artificial structure.
In another embodiment of the method for particle manipulation and sorting based on structural acoustic field according to the present application, the step406 specifically comprises:
The excitation voltage is set to be a fixed value, the ultrasound could be modulated through frequency adjustment. The signal generator produces continuous sinusoidal signals with the voltage of 1500mVpp, the frequency could be increased to 1.282MHz from 1.171MHz, or decreased to 1.171MHz from 1.282MHz, with the step of O.OOlMHz. The sinusoidal signals could excite the ultrasonic transducer through the power amplifier to generate ultrasound wave; the ultrasound excites the artificial structure to vibrate, thus the trapping forces could be produced on the surface of the artificial structure.
One embodiment of the method for particle manipulation and sorting based on structural acoustic field according to the present application, further comprises the following steps:
Step410: Releasing captured particles, and videoing the released particles;
Step412: Measuring the sizes and numbers of the released particles. Whether the diameters of the particles meeting the requirements or not, whether the sorting goal being achieved or not could be inspected with the measurement.
Fig. 5 illustrates the trapping and sorting results of the mixed particles of the two types of glass beads with the diameters of 150-212μιη and 425-600μιη. The signal generator produces continuous sinusoidal signals with the frequency of 1.282MHz, which excites the ultrasonic transducer through the power amplifier to produce ultrasound wave, the ultrasound excites the artificial structure to vibrate, thus the trapping forces could be produced on the surface of the artificial structure. As shown in Fig. 5(a), in case of voltage of 1500mVpp, the surface of the artificial structure videoed by the microscope video module captures two sizes of the glass beads. As shown in Fig. 5(b), when the voltage is decreased to lOOOmVpp, the big glass beads of 425-600μιη fall off the surface of the artificial structure, only the small glass beads of 150-212μιη remain on the surface; as shown in Fig.5(c), when the voltage is decreased to 240mVpp, the small glass beads of 150-212μιη fall off the surface of the artificial structure. Once the driving frequency is fixed, the particles could be captured and sorted by decreasing voltage.
Fig.6 illustrates the trapping and sorting result of three types of glass beads with diameters of <106μιη, 150-212μιη and 425-600μιη. The signal generator produces continuous sinusoidal signals with the frequency of
1.282MHz, which excites the ultrasonic transducer through the power amplifier to produce ultrasound wave, the ultrasound excites the artificial structure to vibrate, thus the trapping forces could be produced on the surface of the artificial structure. As shown in Fig. 6(a), in case of voltage of
220mVpp, the surface of the artificial structure videoed by the microscope video module captures the periodically arrayed glass beads with the diameter of <106μιη ; As shown in Fig. 6(b), when the voltage is increased to 240mVpp, the glass beads of 150-212μιη are trapped onto the surface of the artificial structure and arrayed periodically; as shown in Fig.6(c), when the voltage is increased to lOOOmVpp, the glass beads of 425-600μιη are also trapped onto the surface of the artificial structure, at that time, it can be seen that three sizes of the glass beads are captured onto the surface of the artificial structure. Once the driving frequency is fixed, the particles could be captured and sorted by increasing voltage.
The above is the detailed description to the present application in conjunction with the embodiments, it should be understood that the embodiments are not limits to the above descriptions. For the ordinary skilled in the art, without departing from the ideas of the present application, plurality of simple deductions or replacements could be made to the above embodiments.

Claims

Claims
1. A system for particle manipulation and sorting based on structural acoustic field, comprising: sample holder, ultrasound transmission device and artificial structure, the sample holder is used to place particles to be sorted, the ultrasound transmission device is used to transmit ultrasound wave, the artificial structure is periodic structure used to modulate acoustic field to produce stronger acoustic radiation force and sort the particles to be sorted.
2. The system of claim 1 , wherein the periodic structural includes substrate and a plurality of raised lines in parallel placement on the substrate with equal interval.
3. The system of claim 2, wherein the raised lines are cuboids, polygonal prisms or semi-cylinders.
4. The system of claim 3, wherein the raised lines are cuboids, the spacing between the central lines of each two adjacent cuboids is d, the thickness of the substrate is h2, then 0.15<h2/d<0.25.
5. The system of claim 4, wherein the width, height of the cuboid and the thickness of the substrate are equal.
6. The system of claim 1 -5, wherein the artificial structure uses rigid materials with shear wave velocity more than longitudinal wave band of water used as the substrate.
7. The system of claim 1 -5, wherein the ultrasound transmission device includes signal generator, power amplifier and ultrasonic transducer, the signal generator is used to produce transmission signal, the transmis sion signal excites the ultrasonic transducer via the power amplifier to produce ultrasound wave.
8. The system of claim 7, wherein the signal generator is also used to set the transmission frequency to be the resonance frequency of the artificial structure, realizing acoustic manipulation through voltage adjustment; or the signal generator is used to set the excitation voltage of the signal generator to be a fixed value, realizing acoustic manipulation through frequency adjustment.
9. The system of claim 1-5, further comprising video module and image analysis module, the video module is used to record images of the release of trapped particles, the image analysis module is used to measure the released particles.
10. A method for particle manipulation and sorting based on structural acoustic field, comprising:
Placing particles to be sorted on sample holder, placing artificial structure above the sample holder;
Ultrasound transmission device transmitting ultrasound wave;
The artificial structure modulating the acoustic field;
The artificial structure sorting the particles to be sorted based on the acoustic radiation force produced by the modulation.
11. The method of claim 10, wherein the artificial structure tuning the sound field, further comprising:
Setting the transmission frequency of the signal generator to be the resonance frequency of the artificial structure, realizing the acoustic manipulation through voltage adjustment;
Or setting the excitation voltage of the signal generator to be a fixed value, realizing the acoustic manipulation through frequency adjustment.
12. The method of claim 10, further comprising:
Releasing the captured particles and videoing the released particles; Measuring the released particles.
PCT/CN2013/081208 2013-03-14 2013-08-09 System and method for particle manipulation and sorting based on structural acoustic field WO2014139260A1 (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10245821B2 (en) 2015-12-04 2019-04-02 At&T Intellectual Property I, L.P. Reusable networked 3-D printing
US10780437B2 (en) * 2015-10-10 2020-09-22 Shenzhen Institutes Of Advanced Technology, Chinese Academy Of Sciences Microfluidic system and method of controlling particles based on artificially structured acoustic field
US11209297B2 (en) 2015-10-21 2021-12-28 Texas Instruments Incorporated Ultrasonic transducer system and method using broadband system responses
WO2023283752A1 (en) * 2021-07-12 2023-01-19 中国科学院深圳先进技术研究院 Rotation control module, method, and system

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103203328B (en) * 2013-03-14 2015-08-12 深圳先进技术研究院 The system and method for the manipulation of structure based sound field and screening particle
CN105214742B (en) * 2015-10-10 2017-10-31 中国科学院深圳先进技术研究院 The method of microfluid system and manipulation particulate based on artificial structure's sound field
CN105535968A (en) * 2015-12-11 2016-05-04 中国科学院深圳先进技术研究院 Nanoparticle targeted delivery method based on sound field control
CN107050630B (en) * 2017-04-01 2020-12-08 深圳先进技术研究院 System and method for controlling particles based on time reversal technology
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WO2019119369A1 (en) * 2017-12-21 2019-06-27 深圳先进技术研究院 Acoustic tweezer device
WO2019119368A1 (en) * 2017-12-21 2019-06-27 深圳先进技术研究院 Acoustically driven flow-type cell detection apparatus
CN109946217B (en) * 2017-12-21 2021-08-24 深圳先进技术研究院 Sound-driven flow cytometry detection device
CN109939913B (en) * 2017-12-21 2021-03-23 深圳先进技术研究院 Sound tweezers device
WO2020118688A1 (en) * 2018-12-14 2020-06-18 深圳先进技术研究院 Microfluidic device and methods for manufacturing and using the same
CN110108625B (en) * 2019-05-11 2024-03-26 金华职业技术学院 Adhesion force testing method based on micro-tweezers
CN111530513B (en) * 2020-04-24 2021-06-25 南京师范大学 Acoustic tweezers based on artificial structure is dull and stereotyped
CN113203800B (en) * 2021-05-25 2022-11-18 江苏大学 Performance calibration device and calibration method for interdigital transducer
CN114146890B (en) * 2021-12-03 2022-09-13 深圳先进技术研究院 Ultrasonic sound control method and sound tweezers device
CN114535091B (en) * 2021-12-15 2022-11-29 中国科学院深圳先进技术研究院 Particle sorting method based on sound artificial structure
WO2023108473A1 (en) * 2021-12-15 2023-06-22 中国科学院深圳先进技术研究院 Acoustic artificial structure-based particle sorting method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006031299A2 (en) * 2004-07-29 2006-03-23 The Regents Of The University Of California Ultrasonic analyte concentration and application in flow cytometry
US20080242745A1 (en) * 2007-03-29 2008-10-02 Fujifilm Corporation Aqueous dispersion of metal oxide fine particles and method for producing the same
US20080245709A1 (en) * 2007-04-09 2008-10-09 Gregory Kaduchak Apparatus for separating particles utilizing engineered acoustic contrast capture particles
CN103203328A (en) * 2013-03-14 2013-07-17 深圳先进技术研究院 System and method for controlling and selecting granules on basis of structural sound field

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4212398A (en) * 1978-08-16 1980-07-15 Pet Incorporated Particle separating device
JP2685482B2 (en) * 1988-04-08 1997-12-03 株式会社日立製作所 Method and apparatus for analyzing particulate matter
US7108137B2 (en) * 2002-10-02 2006-09-19 Wisconsin Alumni Research Foundation Method and apparatus for separating particles by size
CN2701537Y (en) * 2004-06-29 2005-05-25 山东强力超声波设备有限公司 Ultrasonic vibrate processor
JP4918771B2 (en) * 2005-09-26 2012-04-18 住友電気工業株式会社 Particle classifying device and adhesive containing particles classified by the device
CN201110824Y (en) * 2007-10-12 2008-09-03 任中京 Dynamic granule image analyzer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006031299A2 (en) * 2004-07-29 2006-03-23 The Regents Of The University Of California Ultrasonic analyte concentration and application in flow cytometry
US20080242745A1 (en) * 2007-03-29 2008-10-02 Fujifilm Corporation Aqueous dispersion of metal oxide fine particles and method for producing the same
US20080245709A1 (en) * 2007-04-09 2008-10-09 Gregory Kaduchak Apparatus for separating particles utilizing engineered acoustic contrast capture particles
CN103203328A (en) * 2013-03-14 2013-07-17 深圳先进技术研究院 System and method for controlling and selecting granules on basis of structural sound field

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CAI, FEIYAN ET AL.: "Acoustic trapping of particle in the near field of a resonant periodically structured stiff plate.", 2011 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM PROCEEDINGS., 21 October 2011 (2011-10-21), pages 1545 - 1547 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10780437B2 (en) * 2015-10-10 2020-09-22 Shenzhen Institutes Of Advanced Technology, Chinese Academy Of Sciences Microfluidic system and method of controlling particles based on artificially structured acoustic field
US11209297B2 (en) 2015-10-21 2021-12-28 Texas Instruments Incorporated Ultrasonic transducer system and method using broadband system responses
US10245821B2 (en) 2015-12-04 2019-04-02 At&T Intellectual Property I, L.P. Reusable networked 3-D printing
US10647106B2 (en) 2015-12-04 2020-05-12 At&T Intellectual Property I, L.P. Reusable networked 3-D printing
WO2023283752A1 (en) * 2021-07-12 2023-01-19 中国科学院深圳先进技术研究院 Rotation control module, method, and system

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