WO2014129774A1 - Procédé d'analyse spectrochimique multi-émission utilisant un miroir dichroïque et spectromètre d'émission l'utilisant - Google Patents

Procédé d'analyse spectrochimique multi-émission utilisant un miroir dichroïque et spectromètre d'émission l'utilisant Download PDF

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Publication number
WO2014129774A1
WO2014129774A1 PCT/KR2014/001266 KR2014001266W WO2014129774A1 WO 2014129774 A1 WO2014129774 A1 WO 2014129774A1 KR 2014001266 W KR2014001266 W KR 2014001266W WO 2014129774 A1 WO2014129774 A1 WO 2014129774A1
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WO
WIPO (PCT)
Prior art keywords
light
dichroic mirror
monochromatic light
target
emission
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PCT/KR2014/001266
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English (en)
Korean (ko)
Inventor
진승민
양일승
서영덕
박효선
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한국화학연구원
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Publication of WO2014129774A1 publication Critical patent/WO2014129774A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/42—Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J2003/1213—Filters in general, e.g. dichroic, band
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices

Definitions

  • It provides a reflection type emission spectroscopic analysis method comprising the step (step 4) of detecting and analyzing the light emission separated in step 3 with a detector.
  • a reflection type emission spectrometer including a detection unit for detecting and analyzing light emission separated by the dichroic mirror.
  • FIG. 4 is a graph measuring wavelengths of light passing through each section of the transmission type emission spectrometer in the sample-free state according to Example 1.
  • FIG. 4 is a graph measuring wavelengths of light passing through each section of the transmission type emission spectrometer in the sample-free state according to Example 1.
  • FIG. 8 is a schematic diagram schematically showing the configuration of a reflection type emission spectrometer according to the present invention.
  • the present invention includes the steps of reflecting the monochromatic light generated by the multi-wavelength monochromatic light source through the first dichroic mirror (step 1);
  • It provides a transmission type emission spectroscopic analysis method comprising the step (step 4) of detecting and analyzing the light emission separated in step 3 with a detector.
  • the dichroic mirror of step 1 is a reflector using a layer of the non-metal material of the flat glass coating and the interference. It is preferable to use a multi-band type dichroic mirror capable of selecting two or more single wavelength range light (monochromatic light) by adjusting a material, a film thickness, and the number of layers to properly select and reflect a part of visible light and then transmit the rest. Do.
  • the method may further include condensing the reflected monochromatic light with an objective lens (step 1 ′).
  • the emitted light is transmitted through the opposite side of the target surface to which the irradiated monochromatic light reaches the target, that is, the other surface of the target.
  • some of the monochromatic light irradiated to the target does not excite the target, and passes through it, and then proceeds in the same traveling direction as the light emitted from the target.
  • the transmission type emission spectroscopy method of the present invention is in the fields of biotechnology and genetic engineering such as DNA structure analysis, DNA sequencing, environmental fields such as the content analysis of organic or inorganic substances in water, chemical reactions and quantum efficiency calculations. It can be usefully used throughout the industry, such as in the field of analytical chemistry, food and agriculture, such as detection of luminescent substances in food, as well as quantification of polymer compounds, film coating.
  • a transmission type emission spectrometer including a detector 208 for detecting and analyzing light emission 207 separated by the second dichroic mirror 206.
  • the light source unit 201 emits a monochromatic light 202 incident on a sample.
  • the light source unit 201 according to the present invention uses a multi-wavelength monochromatic light source consisting of blue, green, and red and their monochromatic light sources. And selectively controlling the light emitting device to emit monochromatic light.
  • the first dichroic mirror 203 is a reflector using multiple layers of flat glass with a non-metallic material and using the interference.
  • the first dichroic mirror 203 adjusts the thickness of the material or the film, the number of layers, and the like to appropriately select a part of the visible light and reflects the rest. It has the property of transmitting.
  • the first dichroic mirror 203 according to the present invention serves to reflect the monochromatic light 202 ′ generated from the light source unit 201, and has an unwanted wavelength to the monochromatic light 202 generated from the light source unit 201. When the light 204 is included, it serves to transmit and remove the light 204 of the unwanted wavelength.
  • the dichroic mirror according to the present invention it is preferable to use a multi-band dichroic mirror capable of selecting two or more single wavelength range light (monochrome light).
  • a multi-wavelength dichroic mirror such as white light
  • the multi-band dichroic mirror selectively reflects a plurality of specific wavelength range light (monochrome light)
  • Light in the other range is characterized by being transmitted. Due to this property, even when the wavelength of the monochromatic light 202 generated from the light source unit 201 is replaced, the sample can be analyzed without mechanical movement of the dichroic mirror.
  • the method may further include an objective lens configured to collect the monochromatic light 202 ′ reflected by the first dichroic mirror 203 and transmit the light to the sample unit 205.
  • the sample unit 205 including the target is to fix the target to be measured
  • the sample unit 205 according to the present invention can be fixed to the liquid or solid target that can transmit the monochromatic light, the position is It is preferable to be located on the same line as the first dichroic mirror 203 and the second dichroic mirror 206.
  • the detector 208 detects and analyzes the light emission 207 separated from the second dichroic mirror 206, and is located on the same line as the sample unit 205 and the second dichroic mirror 206.
  • the light emission 207 separated from the second dichroic mirror 206 is incident.
  • Transmission type luminescence spectroscopy apparatus is a monochromatic filter or monochrome for separating monochromatic light from a light source when performing multi-luminescence spectroscopic analysis by using monochromatic light by multi-wavelength monochromatic light source and multi-wavelength dichroic mirror Luminescence can be measured without mechanical replacement of a single wavelength dichroic mirror in order to measure the change in emission wavelength generated from the sample by the device and a wide range of monochromatic light.
  • the transmission type emission spectroscopy apparatus of the present invention is in the fields of biotechnology and genetic engineering, such as DNA structure analysis, DNA sequencing, environmental fields such as the content analysis of organic or inorganic substances in water, chemical reactions and quantum efficiency calculations. It can be usefully used throughout the industry, such as in the field of analytical chemistry, food and agriculture, such as detection of luminescent substances in food, as well as quantification of polymer compounds, film coating.
  • the present invention comprises the steps of reflecting the monochromatic light generated by the multi-wavelength monochromatic light source through the color-selective mirror (step 1);
  • the monochromatic light reflected in the step 1 reaches the target to excite the target, and obtains the reflective light emission emitted from the excited target (step 2);
  • It provides a reflection type emission spectroscopic analysis method comprising the step (step 4) of detecting and analyzing the light emission separated in step 3 with a detector.
  • the step 1 according to the present invention is a step of reflecting the monochromatic light generated by the multi-wavelength monochromatic light source through the color screening mirror, more specifically, the monochromatic light emitted from the multi-wavelength monochromatic light source of blue, green or red color. After irradiating with the selection mirror, it is the step of reflecting the monochromatic light irradiated to the dichroic mirror to the target of the sample unit.
  • the dichroic mirror of step 1 is a reflector using a layer of the non-metal material of the flat glass coating and the interference. It is preferable to use a multi-band type dichroic mirror capable of selecting two or more single wavelength region light (monochromatic light) by adjusting a material, a film thickness, the number of layers, etc. to appropriately select and reflect a part of visible light and to transmit the rest. Do.
  • the method may further include condensing the reflected monochromatic light with an objective lens (step 1 ′).
  • the objective lens collects the monochromatic light reflected in the step 1 and serves to increase the irradiation amount of the monochromatic light irradiated to the target in the sample unit without dispersing the monochromatic light.
  • the emitted light emits the reflective light in a path that matches the path irradiated with the monochromatic light spatially.
  • some of the monochromatic light irradiated to the target does not excite the target, and passes through it, and then proceeds in the same traveling direction as the light emitted from the target.
  • the step 3 according to the present invention is a step of removing the monochromatic light other than the light emission and separating only the light emission by using the mixed light of the monochromatic light other than the light emission and the light emission obtained in the step 2 by using the color screening mirror of the step 1, More specifically, the mixed light consisting of the emitted light emitted from the target of step 2 and the target monochromatic light that is not excited and irradiated to the color-dividing mirror of step 1 is irradiated to transmit and separate the light, and the target cannot be excited.
  • the monochromatic light is reflected and removed as in step 1 above.
  • step 4 is a step of detecting and analyzing the light emission separated in step 3 with a detector.
  • the reflection type emission spectroscopy method is a monochrome filter for separating monochromatic light from a light source when performing multi-luminescence spectroscopic analysis by using monochromatic light and multi-wavelength dichroic mirror by a multi-wavelength monochromatic light source or Luminescence can be measured without mechanical replacement of a single wavelength dichroic mirror in order to measure the change in emission wavelength generated from the sample by a monochromator and a wide range of monochromatic light.
  • the reflection type emission spectroscopy method of the present invention is in the fields of biotechnology and genetic engineering such as DNA structure analysis, DNA sequencing, environmental fields such as the content analysis of organic or inorganic matter in water, chemical reaction and quantum efficiency calculation. It can be usefully used throughout the industry, such as in the field of analytical chemistry, food and agriculture, such as detection of luminescent substances in food, as well as quantification of polymer compounds, film coating.
  • a dichroic mirror 303 which simultaneously performs a role of reflecting the monochromatic light 302 generated from the light source unit 301 and selecting only the reflective light emission 305 emitted from the sample unit 304;
  • a reflection type emission spectroscopy apparatus including a detection unit 306 for detecting and analyzing light emission 305 separated by the dichroic mirror 303.
  • the light source unit 301 according to the present invention emits a monochromatic light 302 incident on a sample
  • the light source unit 301 according to the present invention is a multi-wavelength monochromatic light source consisting of blue, green and red and their multi-wavelength monochromatic light.
  • the dichroic mirror 303 is a reflector using multiple layers of planar glass as a non-metallic material and using the interference.
  • the dichroic mirror 303 adjusts the thickness of the material or the film, the number of layers, and the like to appropriately select a part of the visible light to reflect the light and transmit the rest. Has characteristics.
  • the dichroic mirror 303 according to the present invention serves to reflect the monochromatic light 302 generated from the light source unit 301.
  • the mixed light including the light emission 305 emitted from the sample unit 304 and the monochromatic light 302 reflected without excitation of the target is transmitted through and separated from the light emission, and the monochromatic light 302 reflected without excitation of the target is separated. Reflects the light and performs only the selection of the reflective light emission 305 at the same time.
  • the dichroic mirror according to the present invention it is preferable to use a multi-band dichroic mirror capable of selecting two or more single wavelength range light (monochrome light).
  • a multi-wavelength dichroic mirror such as white light
  • the multi-band dichroic mirror selectively reflects a plurality of specific wavelength range light (monochrome light), Light in other areas is transmitted. Due to this property, even when the wavelength of the monochromatic light 302 generated from the light source unit 301 is replaced, the sample can be analyzed without mechanical movement of the dichroic mirror.
  • the method may further include an objective lens configured to collect the monochromatic light 302 reflected by the dichroic mirror 304 and transmit it to the sample unit 303.
  • the objective lens serves to increase the irradiation amount of the monochromatic light 302 irradiated to the target in the sample portion 304 without dispersing the monochromatic light 302 by condensing the monochromatic light 302 reflected in step 1,
  • the position is preferably located on the same line of the dichroic mirror 303 and the sample portion 304.
  • the sample unit 304 including the target is to fix the target to be measured
  • the sample unit 304 according to the present invention can be fixed to the solid or liquid target that is impossible to transmit the monochromatic light (302).
  • the detector 306 detects and analyzes the light emission 305 separated from the dichroic mirror 303.
  • the detector 306 is positioned on the same line as the dichroic mirror 306 and the sample unit 304. Light emission 305 separated from 303 is incident.
  • Reflection type luminescence spectroscopy apparatus is a monochromatic filter for separating monochromatic light from a light source when performing a multi-luminescence spectroscopic analysis by using monochromatic light by a multi-wavelength monochromatic light source and multi-wavelength dichroic mirror or Luminescence can be measured without mechanical replacement of a single wavelength dichroic mirror in order to measure the change in emission wavelength generated from the sample by a monochromator and a wide range of monochromatic light.
  • the reflection type luminescence spectroscopy apparatus of the present invention is in the fields of biotechnology and genetic engineering such as DNA structure analysis, DNA sequencing, environmental fields such as the content analysis of organic or inorganic matter in water, chemical reaction and quantum efficiency calculation. It can be usefully used throughout the industry, such as in the field of analytical chemistry, food and agriculture, such as detection of luminescent substances in food, as well as quantification of polymer compounds, film coating.
  • Transmission spectrophotometer (SM240, Korea Spectral Products Co., Ltd.) for measuring the wavelength of the light passing through each component of the transmission type according to the present invention (for example, FIGS. 4 and 6 202, 202 ', 202 ", 204, 207, and 209).
  • SM240 Korea Spectral Products Co., Ltd.
  • FIGS. 4 and 6 202, 202 ', 202 ", 204, 207, and 209 First, the analysis principle of the transmissive luminescence spectrometer in the neglected state is confirmed. In this state, the monochromatic light having a wavelength of about 600 nm to 660 nm is irradiated, the wavelength of the light passing through each section is measured, and the operation principle of the transmissive emission spectrometer is confirmed.

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  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

La présente invention concerne un procédé d'analyse spectrochimique multi-émission utilisant un miroir dichroïque de type multibande, ainsi qu'un multi-spectromètre d'émission l'utilisant. Un spectromètre d'émission du type à transmission selon la présente invention peut utiliser une lumière monochromatique de diverses régions, nécessaire à l'analyse d'échantillons, sans modification mécanique d'un monochromateur, en utilisant une source de lumière monochromatique multi-longueurs d'ondes et un miroir dichroïque multi-longueurs d'ondes, facilitant ainsi considérablement l'analyse d'échantillons nécessitant une analyse en lumière monochromatique multi-longueurs d'ondes et simplifiant un processus de fabrication compte tenu de sa structure simple. De plus, la lumière irradiée traverse le miroir dichroïque avant et après son arrivée sur une cible, de sorte que la lumière monochromatique arrivant sur la cible est séparée de la lumière émise à partir de la cible excitée de façon à améliorer les performances d'analyse du spectromètre. Par conséquent, le spectromètre d'émission peut être employé en biotechnologie et en génie génétique, par exemple dans l'analyse de structures d'ADN, le séquençage d'ADN, etc., dans un domaine environnemental comme l'analyse d'une substance organique ou d'une teneur en minéraux dans l'eau, en chimie analytique comme dans une réaction chimique, un calcul de rendement quantique, etc., et dans le domaine de l'agro-alimentaire, comme pour la détection de substances de luminance dans les aliments, la quantification de composés macromoléculaires, les revêtements en films, etc.
PCT/KR2014/001266 2013-02-20 2014-02-17 Procédé d'analyse spectrochimique multi-émission utilisant un miroir dichroïque et spectromètre d'émission l'utilisant WO2014129774A1 (fr)

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KR10-2013-0018027 2013-02-20
KR1020130018027A KR101675258B1 (ko) 2013-02-20 2013-02-20 색선별 거울을 이용한 멀티발광 분광 분석방법 및 이를 이용한 발광 분광 분석장치

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05180751A (ja) * 1991-12-30 1993-07-23 Toa Medical Electronics Co Ltd 粒子画像分析装置
US5907401A (en) * 1997-09-03 1999-05-25 The United States Of America As Represented By The Secretary Of The Army Device and method for performing an optical hall test
US20070086005A1 (en) * 2001-01-26 2007-04-19 Andreas Gfrorer Optical System and Method for Exciting and Measuring Fluorescence on or in Samples Treated with Fluorescent Pigments
JP2012058105A (ja) * 2010-09-09 2012-03-22 Dkk Toa Corp 光学式分析計
WO2012104496A1 (fr) * 2011-02-04 2012-08-09 Horiba Abx Sas Dispositif et procede de mesures multiparametriques de microparticules dans un fluide

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3820806B2 (ja) * 1999-07-21 2006-09-13 三菱電機株式会社 レーザ検査装置
EP2607889A4 (fr) 2010-08-18 2016-11-09 Nanoentek Inc Microscope à fluorescence pour observation d'image à fluorescence multiple, procédé d'observation d'image à fluorescence l'utilisant, et système d'observation d'image à fluorescence multiple

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05180751A (ja) * 1991-12-30 1993-07-23 Toa Medical Electronics Co Ltd 粒子画像分析装置
US5907401A (en) * 1997-09-03 1999-05-25 The United States Of America As Represented By The Secretary Of The Army Device and method for performing an optical hall test
US20070086005A1 (en) * 2001-01-26 2007-04-19 Andreas Gfrorer Optical System and Method for Exciting and Measuring Fluorescence on or in Samples Treated with Fluorescent Pigments
JP2012058105A (ja) * 2010-09-09 2012-03-22 Dkk Toa Corp 光学式分析計
WO2012104496A1 (fr) * 2011-02-04 2012-08-09 Horiba Abx Sas Dispositif et procede de mesures multiparametriques de microparticules dans un fluide

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KR101675258B1 (ko) 2016-11-14

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