WO2014101885A2

WO2014101885A2 - Device and method for acting on biomaterials by using plasma

Info

Publication number: WO2014101885A2
Application number: PCT/CN2013/091102
Authority: WO
Inventors: 毕鲜荣; 王立言
Original assignee: 无锡思清源生物科技有限公司
Priority date: 2012-12-31
Filing date: 2013-12-31
Publication date: 2014-07-03
Also published as: CN103911279B; WO2014101885A3; CN103911279A

Abstract

The present invention, "a device and a method for acting on biomaterials by using plasma" relates to a biomedical device and is characterized in that: the device comprises a plasma generating device (1) and a clean working bin (2) with no pollutants having biological activity; a sample area (3) is provided within the clean working bin (2) and the working area for plasma generation by the plasma generating device (1) is within the clean working bin (2), wherein the sample area (3) is within the working area; and at least one site for installing a disinfection device is reserved in the cavity or on the wall of the clean working bin. It can be ensured that plasma efficiently and quickly processes the biomaterial without being contaminated by other impurities, microbes, etc., thus having a good application value in the biological field.

Description

Device and method for utilizing plasma to affect biological materials

The present invention relates to a processing device for biological materials, and more particularly to an apparatus and method for utilizing plasma to act on biological materials. Background technique

Plasma is the fourth state of matter, a form of matter consisting of a large number of free electrons and charged ions, and is macroscopically electrically neutral. Charged particles (electrons or ions) in the plasma are usually rich in energy. Studies have shown that a large number of energy-active particles in the plasma can act on biological samples and cause a variety of target trait changes, indicating that it has a good application prospect in the field of biotechnology.

The application of plasma in the biological field is still very rare. Li Guo et al. use a bare electrode discharge to generate a normal pressure chamber. The plasma is processed in an open environment to cause DNA fragmentation. At present, there is also a complete set of plasma disinfection devices for sterilization and sterilization. However, due to the limited penetration ability of the plasma itself and the short working distance, it is necessary to cooperate with other chemicals (such as hydrogen peroxide) in practical applications. , ozone, etc.) to achieve the effect of disinfection.

When plasma acts on whole cells, it can cause strong mutagenic effects of cells. Based on this principle, the use of plasma to breed high-performance mutant strains is one of the important applications of plasma technology in biology. At present, many researchers use different types of plasma to treat different microorganisms, and obtain good mutants, but these experimental devices either process biological samples in an open environment, or although they are relatively closed spaces, However, during the operation, the confined space cannot be sterilized or effectively isolated from the external environment, which may easily lead to contamination of the biological sample, which seriously affects the normal use of the plasma device in research and production. When Yu Hong et al. used the DBD plasma to mutagenize microorganisms, the internal surface of the DBD plasma device was disinfected by hand wiping, but the entire discharge space and the process were not clean. Summary of the invention

The present invention provides a device for using a plasma method to act on a biological sample in a clean working chamber according to the needs and gaps in the above-mentioned fields, and the application of the device to ensure efficient and rapid processing of biological materials. It will not be contaminated by other impurities, microorganisms, etc., and thus has good application value in the biological field. The technical solution of the present invention is as follows: A device for utilizing plasma to act on a biological material, comprising: a plasma generating device (1) and a clean working chamber (2) having no biologically active pollutants; A sample area (3) is disposed in the working chamber (2), and an action area of the plasma generating device (1) generating plasma is in the clean working chamber (2), and the sample area (3) is in the active area At least one disinfecting device installation location is reserved on the inner cavity or wall of the clean working compartment.

The active area refers to a jet region (41) formed by a discharge electrode (40) of the plasma generating device, and the sample region (3) faces the jet region (41).

The relative distance between the sample zone (3) and one end (43) of the discharge electrode forming the jet zone is within (TlOcm).

The relative distance can be adjusted.

A carrier bracket is further included, the carrier bracket is composed of a load end (61) and a mounting end (62), and a bracket leg (63) connecting the load end and the mounting end, the mounting end (62) is used for The load carrier is installed in the clean working chamber (2), the sample area (3) refers to the load end (61), and the load end (61) faces the discharge electrode to form a jet area One end.

A carrier bracket is further included, the carrier bracket is composed of a load end (61) and a mounting end (62), and a bracket leg (63) connecting the load end and the mounting end, the mounting end (62) is used for The carrier bracket is mounted in the clean working compartment (2), the sample zone (3) refers to the cargo end (61), and the bracket leg (63) can be adjusted in length by an adjusting component (64) Thereby, the distance between the carrier end (61) and the discharge electrode (40) is adjusted.

The clean working compartment (2) also has an air inlet (71) and an air outlet (72) for installing the ventilation system.

The air inlet (71) and/or the air outlet (72) are provided with a filtering device (73).

The filtering device (73) meets the requirements of "GB/T14295-2008 air filter" and can effectively filter pollutants such as microorganisms and dust in the air.

The sterile air introduced into the air inlet (71) can ensure the aseptic wind does not interfere with the normal discharge of the plasma and the jet to the biological sample by adding an obstruction in the discharge area or adjusting the position of the inlet and outlet. Processing.

The active area refers to a discharge area (42) between two discharge electrode plates (41) which are hermetically sealed in operation.

The relative distance between the two discharge electrode plates (41) is between 0 and 20 cm.

The two electrode plates (41) are mounted in the clean working chamber (2) and are oppositely disposed to form the discharge region (42). The discharge electrode plate is mounted in the clean working chamber by a bracket, and at least one of the brackets for mounting the discharge electrode plate is adjustable to adjust a relative position between the two discharge electrode plates. Also included is a carrier support (6), which is composed of a load end (61) and a mounting end (62) and a bracket (63) connecting the load end and the mounting end, the mounting end (62) The sample carrier is mounted in the clean working chamber (2), the sample area (3) refers to the load end (61), and the load end (61) is located in the discharge area ( 42) inside.

The disinfecting system is installed at a position of a UV lamp holder (51) preset on the wall of the clean working chamber and a power line through hole (52) of the UV lamp holder. The disinfecting device is an ultraviolet light source.

The disinfecting system is installed at a position of a through hole (53) opened in the wall of the clean working chamber, the disinfecting device is a chemical disinfecting gas supply pipe, and the chemical disinfecting gas supply pipe is connected with a chemical disinfecting gas container (8). The chemical disinfecting gas supply pipe extends into the clean working chamber through the through hole (53) (2).

A sample compartment door is also provided on the wall of the clean work compartment (2).

The selection, properties or mounting position of the selected non-electrode device does not affect the normal discharge of the electrode.

A method of treating a biological material by plasma, characterized in that: treating the biological material with any of the above devices, the plasma generating device generating a plasma and acting on the biological material placed in the sample region.

The gas pressure in the active zone of the plasma generating device is 1 ± 0.5% atmospheric pressure, and the temperature is less than 100 °C. The gas pressure in the working zone where the plasma generating device generates plasma is atmospheric pressure, and the temperature is less than 50 °C.

Prior to plasma treatment of biological materials, the sterilizing system installed in the ventilation system and the sterilizing device installation position has a clean working level of 100,000 or more.

The processing steps are as follows:

(1) Open the sample compartment door

(2) Place the material to be treated in the sample area

(3) Close the sample compartment door

(4) Disinfect the entire clean room by opening the disinfection unit

(5) After disinfection is completed, turn on the ventilation device to evacuate chemical residues (such as ozone, chemical disinfectants, etc.) in the clean work compartment.

(6) Manually or using an automatic device to move and expose the sample to the jet or discharge area of the plasma for processing while ensuring that the sample area and the treatment area are clean.

(7) Start the plasma generator for processing

(8) Collect the processed sample into a closed clean container manually or using an automatic device while ensuring that the sample area is clean.

(9) Take out the closed container through the hatch and use it for later coating, cultivation or screening operations. The invention provides a plasma generating device for processing biological materials with a clean working chamber, comprising a plasma generating device and a clean working chamber; a sample area is arranged in the clean working chamber, and the plasma generating device generates a plasma The action area of the body is in the clean working chamber and facing the sample area; at least one disinfecting device installation position is reserved on the inner cavity or wall of the clean working chamber. It ensures that the plasma acts on the biological material in a clean space. Due to the short plasma interaction distance, the device region of the device of the present invention is disposed within the plasma action region to ensure that the plasma can effectively act on the biological material. In order to achieve a preset cleanliness standard during plasma processing of biological materials in a clean work compartment, the apparatus of the present invention reserves a mounting location for mounting the disinfection apparatus, facilitates installation of a disinfection apparatus associated with the apparatus of the present invention, or installation of other independent Disinfection device.

The plasma generating apparatus employed in the apparatus of the present invention may be currently in common use. In one set of embodiments of the invention, a plasma generating device that produces a plasma jet by coaxial electrode discharge is preferred. In the present invention, the discharge electrode may be located in the clean working chamber at one end or at all, and at least one end is connected to the clean working chamber, and the jet flow region is from the exit from the plasma discharge electrode to the farthest effective end. . Since the plasma penetrating force is limited, in order to achieve an effective effect of the plasma on the biological material, the present invention preferably compares the relative distance between the sample region and the electrode discharge gas outlet.

More preferably, between lmn and TlOcm, the relative distance is adjustable.

The present invention may be to adjust the relative distance by adjusting the mounting manner of the discharge electrode, for example, all extending into the clean working chamber, or only one end is adjacent to the clean working chamber, or the discharging electrode is mounted at a certain height and the position can be adjusted. The above adjustment of the relative distance required is achieved. Or adjusting the relative distance by changing the height of the sample zone. For example, in a preferred embodiment of the set, the clean working compartment includes a carrier bracket, the carrier bracket and the mounting end and the connecting carrier end. And a mounting leg of the mounting end, wherein the mounting end is used to install the carrier bracket in the clean working compartment, the sample area refers to the loading end, and the loading end is opposite to the discharging electrode The jet zone. In order to achieve the above-described relative distance, the relative position can be adjusted arbitrarily by adjusting an opposing position by providing an adjusting member on the leg of the leg, so as to flexibly adjust the working distance of the plasma for different biological materials or different processing purposes.

Further, in a preferred embodiment of the group, the clean working compartment is further provided with an air inlet and an air outlet for the ventilation system. In the case of using a plasma jet zone to treat biological materials, the ventilation system can discharge the chemical residues that are harmful to microorganisms during sterilization; on the other hand, it can also ensure that the clean working compartment is still clean in an open state. That is, the ventilation system continues to operate during the plasma processing of the biological sample to prevent contamination caused by manual or automatic mechanical operations during the treatment.

In one embodiment, it is preferred to provide an obstruction at the upwind of the jet zone so that the sterile wind does not interfere with the discharge of the jet zone and its handling of the biological sample.

In another set of embodiments of the present invention, a preferred plasma generating device is a dielectric barrier discharge (DBD discharge) device, The active area refers to a discharge area between two discharge electrode plates of the DBD discharge device, and the biological material is placed in the discharge area. In a preferred embodiment of the set, the relative distance between the two discharge electrode plates is between 0 and 20 cm to ensure that the plasma is effective for the biological material.

In this set of embodiments, the discharge electrode plates are to be mounted in the clean working chamber and disposed oppositely to form the active area. In some embodiments of the set, the relative distance is adjustable, and to achieve adjustment, at least one of the discharge electrode plates is mounted on the telescopically adjustable bracket.

In some embodiments of the set, the apparatus of the present invention further includes a carrier support, the load carrier being comprised of a load end and a mounting end, and a bracket connecting the load end and the mounting end, the mounting end being used for The carrier bracket is installed in the clean working chamber, the sample area refers to the load end, and the load end is located in the discharge area; and may further preferably be a center position or close to a center position.

In an embodiment of the group, preferably, the clean working chamber (2) is provided with an air inlet (71) and an air outlet (72) for installing a ventilation system, and the working ventilation system can treat the microorganisms generated during sterilization. The chemical residual material is discharged, and then the clean working chamber is sealed for plasma treatment.

In some embodiments of the present invention, the device of the present invention is provided with a disinfecting device, which is an ultraviolet light source or a chemical disinfecting gas supply pipe, and the chemical disinfecting gas supply pipe is connected with a foreign chemical disinfecting gas container.

When the sterilizing device is an ultraviolet light source, the sterilizing system is installed at a position of a purple lamp socket preset on a wall of the clean working chamber and a power line through hole of the ultraviolet lamp socket.

When the sterilizing device is a chemical sterilizing gas supply pipe, the sterilizing system is installed at a position of a through hole opened in a wall of the clean working chamber, and the chemical sterilizing gas supply pipe extends into the clean working chamber through the through hole Inside.

In an embodiment in which the use of the jet zone as the active zone, the clean work compartment is either hermetically sealed or ventilated during operation. In order to achieve the sealing, the connection position between the clean working chamber and the plasma generating device shall be tightly connected; the reserved through hole type mounting position for installing the disinfecting device (for example, the through hole, the air inlet, the air outlet) is installed In the case of these devices, the closed connection is provided, and the closed valve can bring the clean working chamber in the working state to a sealed state; in the case where the above device is not installed, the through holes or the inlet/outlet ports are also provided with a closed valve. The clean work compartment in the working state is brought into a closed state.

In the present invention, in the embodiment in which the discharge zone is used as the active zone, the ventilation system is provided only before the plasma treatment of the biological material, and the airflow in the clean working compartment must be ensured in the treatment process.

The clean working chamber of the present invention has a width of about 4 (T200cm, front and rear width 4 (Tl20cm, height 4 (within Tl00cm. More preferably, left and right width is 120cm, front and rear width is 80cm, and height is 70cm (internal space size).

The present invention also provides a method for treating a biological material by using the above apparatus. During the treatment, it is preferred that the gas pressure in the working region where the plasma generating device generates plasma is atmospheric pressure, and the temperature is less than 100 °C. Further preferably, the temperature is less than 50 °C. Before the plasma treatment of biological materials, the disinfection system installed in the ventilation system and the disinfection system installation position enables the clean work compartment to have a cleanliness level of more than 100,000. The specific steps are as follows:

(1) Open the sample compartment door

(2) Place the material to be treated in the sample area

(3) Close the sample compartment door

(4) Disinfect the entire clean room by opening the disinfection unit

(6) Manually or using an automatic device to move the sample and expose it to the jet or discharge area of the plasma for processing while ensuring that the sample area and the treatment area are clean.

(7) Start the plasma generator for processing

(9) Remove the closed container through the hatch and use it for later coating, cultivation or screening operations.

The level of cleanliness is up to 100,000: It refers to the measurement of suspended particles in the working chamber by using an optical particle counter. The cleanliness of 100,000 or more is in accordance with the following standards: For particles with a diameter of 0.5 μm, the measurement result is less than 3520000/m3 _; diameter 5 Particles of μ m, less than 29300/m3. (Test method for suspended particles in the clean room (area) of the pharmaceutical industry according to GB/T 16292-2010)

Figure 1. The apparatus of the present invention, the active zone being a jet zone, wherein the discharge electrodes are all located in a clean working compartment.

Figure 2. The device of the present invention, the active area is a jet zone, wherein the discharge electrode portion is located in the clean working chamber. Figure 3. The apparatus of the present invention, the active zone being a jet zone, wherein one end of the discharge electrode forming the jet zone is adjacent to the wall of the clean working compartment.

Figure 4. The device of the present invention, the active area is a jet flow area, wherein the discharge electrode is partially located in the clean working chamber, and the sample area is the load end of a load carrier, and the device is provided with an ultraviolet light source socket and a power line pass. hole.

Figure 5. A device according to the present invention, the active region is a jet region, a device of the present invention, the active region is a jet region, wherein the discharge electrode is partially located in a clean working chamber, and the sample region is a cargo end of a carrier. The device is provided with a lamp holder and a power line through hole for installing an ultraviolet light source, and an air inlet and an air outlet are provided.

Figure 6. A device of the present invention, the active zone is a jet zone, a device of the present invention, the active zone is a jet zone, wherein The electric electrode is partially located in the clean working chamber, and the sample area is a loading end of the loading bracket. The loading bracket is telescopically adjustable. The device is provided with a lamp holder and a power line through hole for installing an ultraviolet light source, and is provided with Air inlet and outlet. Figure 7 shows a device of the present invention, the active area is a DBD discharge area, and the sample area is a load end of a carrier holder, and a chemical disinfecting gas through hole is disposed in the apparatus.

In the above drawings: 1 - plasma generating device, 2 - clean working chamber, 3 - sample area. 40—discharge electrode, 41 a jet zone, 42-discharge zone, 51—ultraviolet lamp holder, 52—power line through hole, 53—through hole, 61—loading end, 61—installation end, 63—bracket leg, 64—Adjustment component 71—air inlet, 72—air outlet, 73-filter device, 8—chemical disinfection gas container

The invention is illustrated by the following specific embodiments.

As shown in Figure 7, the apparatus for using a plasma to act on a biological material is characterized by: a plasma generating device (1) and a clean working chamber (2) without biologically active contaminants; a sample area (3) is disposed in the clean working chamber (2), and an active area of the plasma generating device (1) generating plasma is in the clean working chamber (2), and the sample area (3) is in the At least one disinfecting device installation position is reserved in the inner cavity or wall of the clean working chamber. It ensures that the plasma acts on the biological material in a clean space. Due to the short plasma interaction distance, the apparatus of the present invention provides a sample zone within the plasma action zone to ensure that the plasma is effective for the biological material. In order to achieve a preset cleanliness standard during plasma processing of biological materials in a clean work compartment, the apparatus of the present invention reserves a mounting location for mounting the disinfection apparatus, facilitates installation of a disinfection apparatus associated with the apparatus of the present invention, or installation of other independent The disinfection device ensures the cleanliness of the clean work compartment.

The plasma generating apparatus employed in the apparatus of the present invention may be of the type currently used in accordance with an optional plasma generating apparatus, and the embodiments of the present invention are divided into the following two groups: The first set of embodiments:

The plasma generating device is a plasma generating device that introduces a gas between the discharge electrodes and discharges the discharge electrode to generate an ionized gas jet.

In use, the gas to be introduced may be: an inert gas such as helium or argon, or a mixed gas containing helium or argon as a main component.

In the embodiment of the present invention, one end of the discharge electrode generating jet is partially or completely located in the clean working chamber (as shown in FIGS. 1 and 2), and at least one end is adjacent to the clean working chamber (as shown in FIG. 3). The jet zone is from the plasma discharge electrode outlet.

In this embodiment, it is preferable that the relative distance between the sample region (3) and one end (43) of the discharge electrode forming the jet region is 0~10cm.

In the embodiment of the group, it is preferred that the relative distance between the sample zone (3) and one end (43) of the discharge electrode forming the jet zone is adjustable.

In some embodiments, the position of the discharge electrode is adjusted to adjust, for example, all of it into the clean working chamber, or only one end is adjacent to the clean working chamber. In some embodiments, the discharge electrodes are mounted on a bracket of a certain height to achieve the desired relative distance.

In one embodiment, by changing the height of the sample zone, for example, preferably including a carrier support in the clean work compartment, FIGS. 4, 5, and 6 further include a carrier support (6), the load carrier is loaded The object end (61) and the mounting end (62) and the bracket leg (63) connecting the loading end and the mounting end, the mounting end (62) for mounting the carrier bracket (6) in the cleaning work In the cartridge (2), the sample zone (3) refers to the carrier end (61), and the carrier end (61) is opposite to the discharge electrode to form one end of the jet zone. In order to achieve the above-described relative distance, the relative distance can be adjusted by adjusting an opposing member (64) on the leg of the bracket to facilitate flexible adjustment of the plasma working distance for different biological materials or different processing purposes.

Further, in a preferred embodiment of the group, the clean working compartment (2) is also provided with an air inlet (71) and an air outlet (72) for installing the ventilation system. In the case of treating biological materials with a discharge gas in the form of a jet, the ventilation system can discharge microorganisms and chemical residues generated during sterilization, and on the other hand, the clean working chamber can be operated under development, that is, in plasma. The ventilation system is continuously operated during the processing of the biological sample to prevent contamination of the biological sample during manual or automatic operation during the treatment.

In a further optimized embodiment, the air inlet of the present invention is positioned such that the wind direction near the jet zone does not affect the plasma discharge and the interaction between the plasma jet produced thereby and the biological sample.

In a further preferred embodiment of the invention, the air inlet (71) or/and the air outlet (72) are provided with a filtering device (73). Most preferably, the filter unit of the air inlet and/or the air outlet is of a gauge of 100,000 levels.

The process and conditions for the method of treating biological materials using the apparatus obtained in the examples of this group are as follows:

Conditions: The gas pressure in the active zone of the plasma is atmospheric pressure, the temperature is less than 50 ° C, and the gas is high purity helium.

The specific steps are as follows:

(1) Open the sample compartment door

(2) Place the material to be treated in the sample area

(3) Close the sample compartment door

(4) Disinfect the entire clean room by opening the disinfection unit

(5) After the disinfection is completed, open the ventilation device to evacuate the chemical residual substances (such as ozone, chemical disinfectants, etc.) in the clean working compartment. (6) Manually or using an automatic device to move the sample and expose it to the jet area of the plasma for processing while ensuring that the sample area and the treatment area are clean.

(7) Start the plasma generator for processing

(9) Take out the closed container through the hatch and use it for later coating, cultivation or screening operations.

The second set of embodiments:

The plasma generating device is a dielectric barrier discharge (DBD discharge) device as shown in FIG.

In this set of embodiments, the active zone (4) refers to a discharge zone (42) between two discharge electrode plates (41) which are closable under operating conditions.

In some preferred embodiments of the set, the relative distance between the two discharge electrode plates is preferably selected to be between 0 and 20 cm to ensure that the plasma is effective for the biological material.

In this set of embodiments, the two discharge electrode plates (41) are mounted in the clean working chamber (2) and are oppositely disposed to form the discharge region (42).

In some embodiments, the relative distance is adjustable, and to achieve adjustment, at least one of the discharge electrode plates is mounted on the telescopically adjustable bracket. In order to achieve the above-mentioned adjustable relative distance, the apparatus of the present invention further includes a carrier support, the load carrier (61) and the mounting end (62) and the bracket leg connecting the load end and the mounting end (63) The mounting end (62) is for mounting the carrier bracket in the clean working chamber (2), and the sample area (3) refers to the loading end (61), The object end (61) is located within the discharge zone (42).

In an embodiment of the group, preferably, the clean working chamber (2) is provided with an air inlet (71) and an air outlet (72) for installing a ventilation system, and the ventilation system can work on the microorganisms generated during sterilization. The chemical residual material is discharged, and then the clean working chamber is sealed for plasma treatment.

In the above two sets of embodiments of the present invention, some of them are provided with a disinfecting device, and the disinfecting device used in some embodiments is an ultraviolet light source, and the disinfecting system is installed at a position as a preset UV lamp holder on the wall of the clean working chamber ( 51) and a power line through hole (52) of the ultraviolet lamp holder.

In another embodiment, the disinfecting device uses a chemical disinfecting gas supply pipe. The disinfecting system is installed at a position of a through hole (53) opened in the wall of the clean working chamber, and the chemical disinfecting gas supply pipe is connected with a chemical disinfecting gas container (8). The chemical disinfecting gas supply pipe extends into the clean working chamber through the through hole (53) (2).

It should be noted that, in the first group of embodiments, the clean work compartment is either closed or open under working conditions. Wind. In order to achieve the sealing, the connection position between the clean working chamber and the plasma generating device shall be tightly connected; the reserved through hole type mounting position for installing the disinfecting device (for example, the through hole, the air inlet, the air outlet) is installed In the case of these devices, the closed connection is provided, and the closed valve enables the clean working chamber in the working state to be sealed; in the case where the above device is not installed, the through holes or the inlet/outlet ports are also provided with a closed valve. The clean work compartment in the working state is brought into a closed state.

In the second embodiment of the present invention, in the case where a ventilation system is provided, the ventilation system is operated only before the plasma treatment of the biological material, and in the treatment process, it is necessary to ensure that there is no airflow in the clean working chamber.

The material of the clean working chamber of the present invention has a width of about 8 (T200cm, front and rear width 5 (Tl20cm, height 5 (within Tl00cm. More preferably, left and right width is 120cm, front and rear width is 80cm, and height is 70cm (internal space size).

The processes and conditions for the method of treating biological materials using the apparatus of the present invention are as follows:

Conditions: The gas pressure in the active zone of the plasma is atmospheric pressure and the temperature is less than 50 °C.

The specific steps are as follows:

(1) Open the sample compartment door

(2) Place the material to be treated on the load carrier of the work compartment, (3) Close the sample compartment door

(4) Open the disinfection device to disinfect the entire clean work compartment.

(5) After the disinfection is completed, open the ventilation device to evacuate other residual substances in the clean working compartment.

(6) The sample is placed on the carrier holder in the plasma discharge region (7) to start the plasma generating device for processing. Experimental Example 1. Operation process for treating biological materials using the apparatus of the first group of the present invention Experimental materials: Treatment of growth phase logarithmic microbial suspension without pretreatment

Steps:

(1) Open the sample compartment door

(2) Place the material to be treated in the sample area

(3) Close the sample compartment door

(4) Open the disinfection device to disinfect the entire clean work compartment. After the disinfection is completed, open the ventilation device to empty the chemical residues in the clean work compartment to ensure that the cleanliness in the workroom reaches 100,000 or more.

(5) In the case of ensuring that the sample area and the treatment area are clean, manually or using an automatic device to move the sample and expose it to the jet area exposed to the plasma for treatment, the pressure of the plasma in the active area is controlled to atmospheric pressure, and the temperature control is less than 50. °C _;

(6) Start the plasma generator for processing

(7) Collect the treated sample to the sealed clean by hand or using an automatic device while ensuring that the sample area is clean. In a clean container

(8) taking out the closed container through the hatch and using it for post-coating, cultivating or screening operations. Experimental Example 2. Operation of the biological material by the apparatus of the first group of the present invention

Experimental material: Treatment of the growth phase logarithmic microbial suspension without pretreatment

The specific steps are as follows:

(1) Open the sample compartment door

(2) Place the material to be treated on the load carrier of the work compartment,

(3) Close the sample compartment door

(4) Open the disinfection device to disinfect the entire clean work compartment. After the disinfection is completed, open the ventilation device to evacuate other residual substances in the clean workroom, so that the cleanliness level in the workroom can reach 100,000 or above.

(5) Adjust the carrier to move the sample into the plasma discharge zone, and start the plasma generator to process. The gas pressure in the plasma is atmospheric pressure and the temperature is less than 50 °C.

Claims

Claim

A device for utilizing a plasma to act on a biological material, comprising: a plasma generating device (1) and a clean working chamber (2) having no biologically active contaminants; said clean working chamber (2) a sample area (3) is disposed therein, the plasma generating device (1) generates a plasma active area in the clean working chamber (2), and the sample area (3) is in the active area; At least one disinfection device installation location is reserved on the inner chamber or wall of the work compartment.

2. Apparatus according to claim 1, characterized in that said active area refers to a jet zone (41) formed by a discharge electrode (40) of said plasma generating means, said sample zone (3) being facing The jet zone (41).

3. Apparatus according to claim 2, characterized in that the relative distance between the sample zone (3) and one end (43) of the discharge electrode forming the jet zone is within (TlOcm).

4. Apparatus according to claim 3 wherein: said relative distance is adjustable.

5. The apparatus according to claim 3, further comprising: a carrier bracket, the carrier bracket having a load end (61) and a mounting end (62) and a bracket leg connecting the load end and the mounting end (63) constituting that the mounting end (62) is for mounting the carrier bracket in the clean working chamber (2), and the sample area (3) refers to the load end (61), The end of the carrier (61) is facing the discharge electrode to form one end of the jet region

6. The device according to claim 4, further comprising: a carrier support, the carrier support (61) and the mounting end (62) and the support leg connecting the load end and the mounting end (63) constituting that the mounting end (62) is for mounting the carrier bracket in the clean working chamber (2), and the sample area (3) refers to the load end (61), The bracket leg (63) is adjustable in length by the adjusting member (64) to adjust the distance between the carrier end (61) and the discharge electrode (40).

7. Apparatus according to claim 6, wherein the clean working compartment (2) further has an air inlet (71) and an air outlet (72) for installing the ventilation system.

8. Apparatus according to claim 7, wherein the air inlet (71) and/or the air outlet (72) are fitted with filtering means (73).

9. Apparatus according to claim 8, said filtering means (73) said filtering means (73)

The requirements of "GB/T14295-2008 Air Filter" can effectively filter pollutants such as microorganisms and dust in the air.

10. The device according to claim 7, wherein the sterile air introduced at the air inlet (71) can ensure the aseptic air without increasing the obstruction in the discharge area or adjusting the position of the inlet and outlet. Interference with the normal discharge of the plasma and the treatment of the biological sample by the jet.

11. Apparatus according to claim 1, said active zone being a discharge zone (42) between two discharge electrode plates (41), said clean working compartment (2) being hermetically sealed in operation.

The device according to claim 11, characterized by: a relative relationship between the two discharge electrode plates (41) The distance is between 0-20cm. .

13. Apparatus according to claim 11, characterized in that said two electrode plates (41) are mounted in said clean working chamber (2) and are arranged oppositely to form said discharge zone (42).

The device according to any one of claims 1 to 13, wherein: the discharge electrode plate is installed in the clean working chamber through a bracket, and at least one of the brackets for mounting the discharge electrode plate is adjustable To adjust the relative position between the two discharge electrode plates.

15. Apparatus according to claim 14, further comprising a carrier support (6), the load carrier (61) and the mounting end (62) and the connection end and mounting a bracket (63) at the end, the mounting end

(62) for mounting the carrier bracket in the clean working chamber (2), the sample area (3) refers to the load end (61), and the load end (61) is located at Inside the discharge zone (42).

16. Apparatus according to any of claims 13 to 15, characterized in that the clean working compartment (2) is also provided with an air inlet (71) and an air outlet (72) for installing the ventilation system.

17. The device according to any one of claims 16, wherein: the disinfecting system is installed at a position of a UV lamp holder (51) preset on a wall of the clean working chamber and the UV lamp holder A power line through hole (52), the sterilizing device is an ultraviolet light source.

18. The device according to any one of claims 16, wherein: the disinfecting system is installed in a through hole (53) opened in the wall of the clean working chamber, and the disinfecting device is a chemical disinfecting gas supply pipe. The chemical disinfecting gas supply pipe is externally connected to the chemical disinfecting gas container (8), and the chemical disinfecting gas supply pipe extends into the clean working chamber through the through hole (53) (2).

19. Apparatus according to claim 17 or 18, wherein the clean working compartment (2) is further provided with a sample hatch on the wall.

20. A method according to claim 19, wherein the selection, properties or mounting position of the selected non-electrode device does not affect the normal discharge of the electrode.

A method of treating a biological material by using a plasma, comprising: treating the biological material with the apparatus according to any one of claims 19, wherein the plasma generating device generates a plasma and acts on the sample region. biomaterials.

The method according to claim 20, wherein the plasma generating device generates a plasma having a gas pressure of 1 ± 0.5% atmospheric pressure and a temperature lower than 100 °C.

The method according to claim 20, wherein the plasma generating device generates a plasma in which the gas pressure is atmospheric pressure and the temperature is less than 50 °C.

23. The method according to claim 20, wherein: before the plasma treatment of the biological material, the disinfection device installed at the installation position of the disinfecting device is used to make the clean working chamber have a cleanliness level of 100,000 or more.

24. The method of claim 20, wherein: the processing steps are as follows:

(1) Open the sample compartment door

(2) Place the material to be treated in the sample area

(3) Close the sample compartment door

(4) Disinfect the entire clean room by opening the disinfection unit

(7) Start the plasma generator for processing