WO2014087055A1 - Method for detecting helicobacter pylori dna in a stool sample - Google Patents
Method for detecting helicobacter pylori dna in a stool sample Download PDFInfo
- Publication number
- WO2014087055A1 WO2014087055A1 PCT/FI2013/051144 FI2013051144W WO2014087055A1 WO 2014087055 A1 WO2014087055 A1 WO 2014087055A1 FI 2013051144 W FI2013051144 W FI 2013051144W WO 2014087055 A1 WO2014087055 A1 WO 2014087055A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- seq
- set forth
- nucleotide sequence
- pylori
- oligonucleotide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Definitions
- Clarithromycin resistance of H. pylori is well known and due to point mutations within the
- oligonucleotide sequences that enable reliable species- specific amplification, detection and quantification. It is of utmost importance that a given set of oligonucleotides, designed to amplify H. pylori, does not cross-react with DNA originating from any other species possibly present in a sample.
- the present invention provides a method of detecting H. pylori DNA in a stool sample, the method comprising the steps of: a) performing a PCR reaction comprising DNA isolated from a stool sample as a template and an oligonucleotide primer set specific for amplifying an H. pylori-specific target sequence in an H.
- the oligonucleotide primer set comprises an oligonucleotide consisting of at least 10 contiguous nucleotides present in a nucleotide sequence as set forth in SEQ ID NO: 1 and an oligonucleotide consisting of at least 10 contiguous nucleotides present in a nucleotide sequence as set forth in SEQ ID NO: 2; and b) detecting H. pylori DNA in said stool sample when the H. pylori -specific target sequence is amplified.
- the present invention is further directed to a kit for detecting H. pylori DNA in a stool sample, the kit comprising the oligonucleotide primer set as described above; and a reagent for performing amplification of a nucleic acid in a PCR reaction.
- oligonucleotide refers to any polymer of two or more of nucleotides, nucleosides, nucleobases or related compounds used as a reagent in the DNA amplification methods of the present invention.
- the oligonucleotide may be DNA and/or RNA and/or analogs thereof.
- the term oligonucleotide does not denote any particular function to the reagent; rather, it is used generically to cover all such reagents described herein. Specific oligonucleotides of the present invention are described in more detail below.
- an oligonucleotide can be virtually any length, limited only by its specific function in the DNA amplification reaction.
- Oligonucleotides of a defined sequence and chemical structure may be produced by techniques known to those of ordinary skill in the art, such as by chemical or biochemical synthesis, and by in vitro or in vivo expression from recombinant nucleic acid molecules, e.g., bacterial or viral vectors. Oligonucleotides may be modified in any way, as long as a given modification is compatible with the desired function of a given oligonucleotide. One of ordinary skill in the art can easily determine whether a given modification is suitable or desired for any given oligonucleotide of the present invention. Modifications include, but are not limited to base modifications, sugar modifications or backbone modifications.
- the amplification product contains a sequence having sequence identity with a target nucleic acid sequence or its complement and can be detected with, for example, an intercalating dye or a detection probe having specificity for a region of the target nucleic acid sequence or its complement.
- the PCR reaction of the present invention is preferably performed as a real-time PCR assay.
- the term "probe" refers to any of a variety of signaling molecules indicative of amplification.
- SYBR ® Green and other DNA -binding dyes are detector probes.
- Some detector probes can be sequence-based, for example 5' nuclease probes.
- Various detector probes are known in the art, for example TaqMan ® probes (See U.S. Patent No.
- H. pylori DNA is present only in low level in feces, since H. pylori is an upper intestinal track pathogen. Therefore, the right choice of highly specific and sensitive primers is of crucial importance in order to obtain accurate results from a PCR based assay using DNA template isolated from a stool sample.
- the present invention provides a method and an oligonucleotide primer set for amplifying at least one target sequence of the 23S rRNA gene of H. pylori.
- the developed assay can detect H. pylori infection with high sensitivity and simultaneously evaluate clarithromycin resistance.
- the effect of the invention is particularly related to the choice of target sites for the outer primers, i.e. SEQ ID NOS: 1 and 2.
- the present invention is directed to a method of detecting H. pylori DNA in a stool sample, the method comprising the steps of: a) performing a PCR reaction comprising DNA isolated from a stool sample as a template and an oligonucleotide primer set specific for amplifying an H. py lori- specific target sequence in an H.
- a second oligonucleotide primer set can be used in said PCR reaction comprising an oligonucleotide consisting of at least 10 contiguous nucleotides present in a nucleotide sequence as set forth in SEQ ID NO: 3 and an oligonucleotide consisting of at least 10 contiguous nucleotides present in a nucleotide sequence as set forth in SEQ ID NO: 4.
- Said second oligonucleotide primer set is directed to the amplification of sites of point mutations associated with clarithromycin resistance of certain H. pylori strains.
- One of the most preferred embodiments for the present invention is to perform steps a) and b) of the method in a single vessel (e.g. as described by Strauss et al., 2000). This approach lowers the risk of contamination.
- the melting temperatures of the first and second oligonucleotide primer set must be designed so that the temperature difference between the sets is at least 3 degrees centigrade, preferably 3.5 or 4 degrees centigrade.
- the target sequence in H. pylori 23S rRNA gene for the first oligonucleotide primer set consisting of SEQ ID NOS: 1 and 2 corresponds to positions 1937-2793 of SEQ ID NO: 13.
- the target sequence in H. pylori 23S rRNA gene for the second oligonucleotide primer set consisting of SEQ ID NOS: 3 and 4 corresponds to positions 2482-2624 of SEQ ID NO: 13.
- the present invention also provides an oligonucleotide primer set comprising an oligonucleotide consisting of at least 10 contiguous nucleotides present in a nucleotide sequence as set forth in SEQ ID NO: 1 and an oligonucleotide consisting of at least 10 contiguous nucleotides present in a nucleotide sequence as set forth in SEQ ID NO: 2, wherein the oligonucleotide primer set amplifies a target sequence in a 23S rRNA gene of H. pylori.
- the oligonucleotide primer set comprises or consists of the nucleotide sequence as set forth in SEQ ID NO: 1 and the nucleotide sequence as set forth in SEQ ID NO: 2.
- the present invention is also providing a kit for detecting H. pylori DNA in a stool sample, the kit comprising at least one of the oligonucleotide primer sets as described above; and a reagent for performing amplification of a nucleic acid in a PCR reaction.
- said reagent is selected from a group consisting of DNA polymerase, dNTPs, and a buffer.
- the total nucleid acids were extracted using bioMerieux NucliSens Kits, and semi- automated easyMAG instrument for extraction. Both generic and specific B protocol was successfully tested. The specific B protocol was slightly better in qPCR performance, and it was selected for all experiments. A loopful of stool, app. 10% solution, was inoculated into 2mL of the kit lysis buffer, mixed rigorously for 5s and incubated at least 15min in room temperature according to manufacturer's instruction. The extraction volume was 25 ⁇ 1. PCR setup and oligonucleotides
- Hpy_sel_003_ R GCTTGTGCCATTACACTCAACTTGCGATTTC (SEQ ID NO:2)
- R_Hpyin_06 CAAGGATGGCTCCATAAG (SEQ ID NO:4)
- the underlined nucleotides in the above list are LNA nucleotides (Exiqon A/S) increasing the Tm of the probe.
- ROX red: a mutation in A2142G or A2143G position linked to clarithromycin resistance. The most common types detected.
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP13814189.0A EP2929051B1 (en) | 2012-12-05 | 2013-12-05 | Method for detecting helicobacter pylori dna in a stool sample |
JP2015546070A JP6574703B2 (en) | 2012-12-05 | 2013-12-05 | Method for detecting Helicobacter pylori DNA in stool samples |
CN201380069820.XA CN104919058B (en) | 2012-12-05 | 2013-12-05 | Method for detecting the helicobacter pylori DNA in excrement sample |
ES13814189.0T ES2664335T3 (en) | 2012-12-05 | 2013-12-05 | Method to detect Helicobacter pylori DNA in a stool sample |
US14/649,674 US9868995B2 (en) | 2012-12-05 | 2013-12-05 | Method for detecting Helicobacter pylori DNA in a stool sample |
DK13814189.0T DK2929051T3 (en) | 2012-12-05 | 2013-12-05 | PROCEDURE FOR DETECTING HELICOBACTER PYLORI DNA IN A FAILURE TEST |
CA2893941A CA2893941C (en) | 2012-12-05 | 2013-12-05 | Method for detecting helicobacter pylori dna in a stool sample |
PL13814189T PL2929051T3 (en) | 2012-12-05 | 2013-12-05 | Method for detecting helicobacter pylori dna in a stool sample |
AU2013353904A AU2013353904B2 (en) | 2012-12-05 | 2013-12-05 | Method for detecting Helicobacter pylori DNA in a stool sample |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI20126271 | 2012-12-05 | ||
FI20126271 | 2012-12-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2014087055A1 true WO2014087055A1 (en) | 2014-06-12 |
Family
ID=49883127
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/FI2013/051144 WO2014087055A1 (en) | 2012-12-05 | 2013-12-05 | Method for detecting helicobacter pylori dna in a stool sample |
Country Status (11)
Country | Link |
---|---|
US (1) | US9868995B2 (en) |
EP (1) | EP2929051B1 (en) |
JP (1) | JP6574703B2 (en) |
CN (1) | CN104919058B (en) |
AU (1) | AU2013353904B2 (en) |
CA (1) | CA2893941C (en) |
DK (1) | DK2929051T3 (en) |
ES (1) | ES2664335T3 (en) |
PL (1) | PL2929051T3 (en) |
PT (1) | PT2929051T (en) |
WO (1) | WO2014087055A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ITUB20160413A1 (en) * | 2016-02-05 | 2017-08-05 | Thd Spa | Method for determining Helicobacter pylori |
CN107227374A (en) * | 2017-08-01 | 2017-10-03 | 扬州大学 | A kind of primer, kit and authentication method that Rodent Helicobacter is identified based on quantitative fluorescent PCR |
RU2651033C1 (en) * | 2017-04-17 | 2018-04-18 | Федеральное государственное бюджетное научное учреждение "Федеральный исследовательский центр "Красноярский научный центр Сибирского отделения Российской академии наук" (ФИЦ КНЦ СО РАН) | Method for diagnosing chronic gastritis associated with helicobacter pylori |
RU2664449C1 (en) * | 2017-05-29 | 2018-08-17 | Наталия Васильевна Журбина | Method for predicting the exacerbations of gastroduodenal ulcer associated with helicobacter pylori |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106119372A (en) * | 2016-07-05 | 2016-11-16 | 首都医科大学 | A kind of method detecting meriones unguiculatus infection helicobacter pylori |
CN107201410A (en) * | 2017-07-26 | 2017-09-26 | 孙晓彦 | ARMS qPCR methods and kit for helicobacter pylori individuation genetic test |
CN110669848B (en) * | 2018-07-03 | 2022-11-15 | 北京福安华生物科技有限公司 | Artificial simulated molecular beacon and kit for detecting helicobacter pylori |
CN111910011A (en) * | 2019-05-10 | 2020-11-10 | 江苏康为世纪生物科技有限公司 | Kit for detecting drug-resistant mutation sites of helicobacter pylori |
CN111944916A (en) * | 2020-09-14 | 2020-11-17 | 壹宏(深圳)基因有限公司 | Reagent for fecal microorganism detection and application thereof in gastric helicobacter pylori detection |
CN115627297B (en) * | 2022-12-05 | 2023-04-11 | 北京吉检医疗科技有限公司 | Kit for detecting human excrement helicobacter pylori nucleic acid |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
JP2005168474A (en) | 2003-12-15 | 2005-06-30 | Morinaga Milk Ind Co Ltd | Method for testing clarithromycin resistance of helicobacter pylori |
JP2006020511A (en) * | 2004-07-06 | 2006-01-26 | Toyobo Co Ltd | Method for detecting nucleic acid |
WO2007106407A2 (en) * | 2006-03-10 | 2007-09-20 | Wyeth | Microarray for monitoring gene expression in multiple strains of streptococcus pneumoniae |
CN101665824A (en) * | 2009-09-24 | 2010-03-10 | 周玉贵 | Biochip for detecting drug resistant genes of helicobacter pylori clarithromycin and preparation method and application thereof |
JP2010233505A (en) * | 2009-03-31 | 2010-10-21 | Toyobo Co Ltd | Reagent kit for detecting nucleic acid amplification, having excellent preservation stability |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH10286099A (en) * | 1997-04-16 | 1998-10-27 | S R L:Kk | Primer for detecting helicobacter pylori bacterium and examination of clarithromycin resistance of helicobacter pylori bacterium with the same |
JP2006271290A (en) * | 2005-03-30 | 2006-10-12 | Toyobo Co Ltd | New oligonucleotide primer and method for detecting point mutation with the same |
JP5593582B2 (en) * | 2007-06-12 | 2014-09-24 | 東洋紡株式会社 | Rapid detection method of nucleic acid |
JP2011062143A (en) | 2009-09-17 | 2011-03-31 | Arkray Inc | Primer reagent for amplifying 23s rrna gene of helicobacter pylori and use of the same |
-
2013
- 2013-12-05 CA CA2893941A patent/CA2893941C/en active Active
- 2013-12-05 CN CN201380069820.XA patent/CN104919058B/en active Active
- 2013-12-05 AU AU2013353904A patent/AU2013353904B2/en active Active
- 2013-12-05 EP EP13814189.0A patent/EP2929051B1/en active Active
- 2013-12-05 JP JP2015546070A patent/JP6574703B2/en active Active
- 2013-12-05 PL PL13814189T patent/PL2929051T3/en unknown
- 2013-12-05 DK DK13814189.0T patent/DK2929051T3/en active
- 2013-12-05 WO PCT/FI2013/051144 patent/WO2014087055A1/en active Application Filing
- 2013-12-05 ES ES13814189.0T patent/ES2664335T3/en active Active
- 2013-12-05 PT PT138141890T patent/PT2929051T/en unknown
- 2013-12-05 US US14/649,674 patent/US9868995B2/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
JP2005168474A (en) | 2003-12-15 | 2005-06-30 | Morinaga Milk Ind Co Ltd | Method for testing clarithromycin resistance of helicobacter pylori |
JP2006020511A (en) * | 2004-07-06 | 2006-01-26 | Toyobo Co Ltd | Method for detecting nucleic acid |
WO2007106407A2 (en) * | 2006-03-10 | 2007-09-20 | Wyeth | Microarray for monitoring gene expression in multiple strains of streptococcus pneumoniae |
JP2010233505A (en) * | 2009-03-31 | 2010-10-21 | Toyobo Co Ltd | Reagent kit for detecting nucleic acid amplification, having excellent preservation stability |
CN101665824A (en) * | 2009-09-24 | 2010-03-10 | 周玉贵 | Biochip for detecting drug resistant genes of helicobacter pylori clarithromycin and preparation method and application thereof |
Non-Patent Citations (28)
Title |
---|
BOOKA ET AL., HELICOBACTER, vol. 10, 2005, pages 205 - 213 |
BURUCOA ET AL., JOURNAL OF CLINICAL MICROBIOLOGY, vol. 37, no. 12, 1999, pages 4071 - 4080 |
C. FONTANA ET AL: "Detection of Clarithromycin-Resistant Helicobacter pylori in Stool Samples", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 41, no. 8, 1 August 2003 (2003-08-01), pages 3636 - 3640, XP055098250, ISSN: 0095-1137, DOI: 10.1128/JCM.41.8.3636-3640.2003 * |
C. SCHABEREITER-GURTNER ET AL: "Novel Real-Time PCR Assay for Detection of Helicobacter pylori Infection and Simultaneous Clarithromycin Susceptibility Testing of Stool and Biopsy Specimens", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 42, no. 10, 1 October 2004 (2004-10-01), pages 4512 - 4518, XP055098248, ISSN: 0095-1137, DOI: 10.1128/JCM.42.10.4512-4518.2004 * |
CHEY; WONG, AM J GASTROENTEROL, vol. 102, 2007, pages 1808 - 1825 |
DEWHIRST ET AL., JOURNAL OF BACTERIOLOGY, vol. 187, no. 17, 2005, pages 6106 - 6118 |
EMIKO RIMBARA ET AL: "Development of a Highly Sensitive Method for Detection of Clarithromycin-Resistant Helicobacter pylori from Human Feces", CURRENT MICROBIOLOGY, SPRINGER-VERLAG, NE, vol. 51, no. 1, 1 July 2005 (2005-07-01), pages 1 - 5, XP019365588, ISSN: 1432-0991 * |
FALSAFI ET AL., WORLD J GASTROENTEROL, vol. 15, no. 4, 2009, pages 484 - 488 |
FISCHBACH; EVANS, ALIMENT PHARMACOL THER, vol. 26, 2007, pages 343E57 |
FONTANA ET AL., JOURNAL OF CLINICAL MICROBIOLOGY, vol. 41, no. 8, 2003, pages 3636 - 3640 |
FORD ET AL., AM J GASTROENTEROL, vol. 99, 2004, pages 1833 - 1855 |
FORD ET AL., THE COCHRANE DATABASE OF SYSTEMATIC REVIEWS, vol. 4, 2003 |
INNIS ET AL.: "PCR Protocols: A Guide to Methods and Applications", 1990, ACADEMIC PRESS |
J. Z. XING: "Development of a Microelectronic Chip Array for High-Throughput Genotyping of Helicobacter Species and Screening for Antimicrobial Resistance", JOURNAL OF BIOMOLECULAR SCREENING, vol. 10, no. 3, 1 April 2005 (2005-04-01), pages 235 - 245, XP055098253, ISSN: 1087-0571, DOI: 10.1177/1087057104273781 * |
KHAN ET AL., ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 48, no. 9, 2004, pages 3567 - 3569 |
KOSUNEN ET AL., INT. J. CANCER, vol. 128, 2011, pages 433 - 439 |
LOTTSPEICH, JOURNAL OF CLINICAL MICROBIOLOGY, vol. 45, no. 6, 2007, pages 1718 - 1722 |
MAEDA ET AL., GUT, vol. 43, 1998, pages 317 - 321 |
MAKRISTATHIS; 1998 ET AL., JOURNAL OF CLINICAL MICROBIOLOGY, vol. 36, no. 9, pages 2772 - 2774 |
MALFERTHEINER ET AL., GUT, vol. 61, 2012, pages 646E664 |
MISHRA ET AL., J INFECT DEVELOPING COUNTRIES, vol. 2, no. 3, 2008, pages 206 - 210 |
MONTEIRO ET AL., JOURNAL OF MICROBIOLOGICAL METHODS, vol. 45, 2001, pages 89 - 94 |
NOGUCHI ET AL., JOURNAL OF MEDICAL MICROBIOLOGY, vol. 56, 2007, pages 1174 - 1180 |
RIMBARA ET AL., CURRENT MICROBIOLOGY, vol. 51, 2005, pages 1 - 5 |
SCALETSKY ET AL., HELICOBACTER, vol. 16, 2011, pages 311 - 315 |
SCHABEREITER-GURTNER ET AL., JOURNAL OF CLINICAL MICROBIOLOGY, vol. 42, no. 10, 2004, pages 4512 - 4518 |
SINGH ET AL., HELICOBACTER, vol. 13, no. 1, 2008, pages 30 - 34 |
STRAUSS ET AL., DIAGNOSTIC MOLECULAR PATHOLOGY, vol. 9, no. 3, 2000, pages 151 - 157 |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ITUB20160413A1 (en) * | 2016-02-05 | 2017-08-05 | Thd Spa | Method for determining Helicobacter pylori |
WO2017134627A1 (en) * | 2016-02-05 | 2017-08-10 | Thd S.P.A. | Method for determining helicobacter pylori |
CN108884489A (en) * | 2016-02-05 | 2018-11-23 | Thd股份公司 | Method for determining helicobacter pylori (Helicobacter pylori) |
RU2744190C1 (en) * | 2016-02-05 | 2021-03-03 | ТХД С.п.А. | Method for determining helicobacter pylori |
CN108884489B (en) * | 2016-02-05 | 2022-06-14 | Thd股份公司 | Method for determining Helicobacter pylori (Helicobacter pylori) |
US11377681B2 (en) | 2016-02-05 | 2022-07-05 | Thd S.P.A. | Method for determining Helicobacter pylori |
RU2651033C1 (en) * | 2017-04-17 | 2018-04-18 | Федеральное государственное бюджетное научное учреждение "Федеральный исследовательский центр "Красноярский научный центр Сибирского отделения Российской академии наук" (ФИЦ КНЦ СО РАН) | Method for diagnosing chronic gastritis associated with helicobacter pylori |
RU2664449C1 (en) * | 2017-05-29 | 2018-08-17 | Наталия Васильевна Журбина | Method for predicting the exacerbations of gastroduodenal ulcer associated with helicobacter pylori |
CN107227374A (en) * | 2017-08-01 | 2017-10-03 | 扬州大学 | A kind of primer, kit and authentication method that Rodent Helicobacter is identified based on quantitative fluorescent PCR |
Also Published As
Publication number | Publication date |
---|---|
CA2893941A1 (en) | 2014-06-12 |
US20160017406A1 (en) | 2016-01-21 |
CN104919058B (en) | 2017-11-21 |
CA2893941C (en) | 2021-05-18 |
PT2929051T (en) | 2018-04-10 |
US9868995B2 (en) | 2018-01-16 |
CN104919058A (en) | 2015-09-16 |
AU2013353904B2 (en) | 2017-07-13 |
ES2664335T3 (en) | 2018-04-19 |
AU2013353904A1 (en) | 2015-07-09 |
JP2015536676A (en) | 2015-12-24 |
EP2929051A1 (en) | 2015-10-14 |
JP6574703B2 (en) | 2019-09-11 |
PL2929051T3 (en) | 2018-07-31 |
DK2929051T3 (en) | 2018-04-16 |
EP2929051B1 (en) | 2018-01-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2013353904B2 (en) | Method for detecting Helicobacter pylori DNA in a stool sample | |
US20220064715A1 (en) | Polymerase Chain Reaction Primers and Probes for Mycobacterium Tuberculosis | |
CN109055502B (en) | Detection method, detection kit and application of invasive fungal infection | |
Xuan et al. | Detection of clarithromycin-resistant Helicobacter pylori in clinical specimens by molecular methods: A review | |
US20110287965A1 (en) | Methods and compositions to detect clostridium difficile | |
Clancy et al. | Development of internally controlled duplex real-time NASBA diagnostics assays for the detection of microorganisms associated with bacterial meningitis | |
Debruyne et al. | Comparative performance of different PCR assays for the identification of Campylobacter jejuni and Campylobacter coli | |
JP2007274934A (en) | Primer set and method for detecting food poisoning bacterium | |
Couzinet et al. | High-density DNA probe arrays for identification of staphylococci to the species level | |
KR20190037027A (en) | Primer set for detection of SFTSV and SFTSV detection method using the same | |
AU2021269432B2 (en) | Method for detecting the presence of a hypervirulent Clostridium difficile strain | |
CN111712583B (en) | Method for diagnosing tsutsugamushi disease using multiple copy genes | |
WO2012066576A2 (en) | Oligonucleotide primer sequences for detection of leptospira | |
Mahajan et al. | Molecular Techniques for Diagnosis of Systemic Fungal Infections | |
JP2006075139A (en) | PRIMER FOR DETECTION AND TYPE DISCRIMINATION OF cagA GENE OF HELICOBACTER PYLORI EXISTING IN SAMPLE, METHOD AND KIT | |
Prabhraj et al. | OIE recommended nucleic acid based techniques for poultry diseases diagnosis | |
WO2013137820A1 (en) | Methods and systems for the detection of methicillin resistant staphylococcus aureus |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 13814189 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 2015546070 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 14649674 Country of ref document: US |
|
ENP | Entry into the national phase |
Ref document number: 2893941 Country of ref document: CA |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2013814189 Country of ref document: EP |
|
ENP | Entry into the national phase |
Ref document number: 2013353904 Country of ref document: AU Date of ref document: 20131205 Kind code of ref document: A |