WO2014077601A1 - On-chip integrated unit for cell culture observation, and on-chip integrated kit for cell culture observation - Google Patents

On-chip integrated unit for cell culture observation, and on-chip integrated kit for cell culture observation Download PDF

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WO2014077601A1
WO2014077601A1 PCT/KR2013/010347 KR2013010347W WO2014077601A1 WO 2014077601 A1 WO2014077601 A1 WO 2014077601A1 KR 2013010347 W KR2013010347 W KR 2013010347W WO 2014077601 A1 WO2014077601 A1 WO 2014077601A1
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cell culture
observation
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이도영
김송이
변경수
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(주)실리콘화일
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    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

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  • the present invention relates to a cell culture observation apparatus capable of observing cell culture and cultured cells, and in particular, an on-chip cell having an observation part used for observing cultured cells and a cell culture part for culturing cells on the observation part. It relates to a culture observation integrated unit.
  • Cell culture can be broadly classified into a cell culture step of culturing cells by placing a culture plate containing cells in an incubator and a monitoring step of observing and analyzing the culture process and differentiation characteristics of the cells.
  • the environment inside the incubator where the cell culture proceeds and where the observation equipment where the observation proceeds is installed is quite different. Thus, during observation, the cells are stressed or contaminated, and an error occurs when observing changes caused by specific stimuli to the cells.
  • the technical problem to be solved by the present invention is to provide an on-chip cell culture observation integrated unit that implements the incubator and observation equipment in one chip.
  • Another technical problem to be solved by the present invention is to provide an on-chip cell culture observation integration kit using the on-chip cell culture observation integration unit.
  • On-chip cell culture observation integrated unit includes one observation unit and one cell culture unit.
  • the observation portion is formed on the surface of the substrate.
  • the cell culture part is formed on the upper part of the observation part to culture the cells, and is surrounded by a partition of a constant height.
  • On-chip cell culture observation integrated unit includes one observation unit and a plurality of cell culture unit.
  • the observation portion is formed on the surface of the substrate.
  • the cell culture unit is formed on the observation unit and cultures the cells.
  • the plurality of cell culture parts are enclosed by a single partition wall having a constant height.
  • On-chip cell culture observation integrated unit for achieving the above technical problem, comprises a plurality of observation and a plurality of cell culture.
  • the observation portion is formed on the surface of the substrate.
  • the cell culture unit is formed on top of the plurality of observation units, respectively, to culture the cells.
  • Each of the cell culture units is surrounded by a partition wall having a constant height.
  • the on-chip cell culture observation integrated unit and the on-chip cell culture observation integrated kit according to the present invention can easily observe the growth stage and differentiation characteristics of cells cultured on the surface of the sensor by using the signal detected by the sensor. There is an advantage.
  • FIG 1 is an embodiment of an on-chip cell culture observation integrated unit according to the present invention.
  • Figure 2 is another embodiment of the on-chip cell culture observation integrated unit according to the present invention.
  • Figure 3 is an embodiment of an on-chip cell culture observation integrated kit according to the present invention.
  • Figure 4 is another embodiment of the on-chip cell culture observation integrated kit according to the present invention.
  • FIG 1 is an embodiment of an on-chip cell culture observation integrated unit according to the present invention.
  • On-chip cell culture observation integrated unit 100 includes an observation unit 130 and the cell culture unit 150.
  • the observation unit 130 is formed on the substrate 110 and includes a plurality of image sensors pixels 140.
  • the cell culture unit 150 is formed on the observation unit 130 and is surrounded by the partition wall 120 having a predetermined height.
  • Each image sensor pixel 140 includes a photodiode (not shown) for generating a charge corresponding to light incident through a cell cultured in the cell culture unit 150 and an electricity corresponding to a charge generated from the photodiode. And a conversion circuit for generating a signal. Since the image sensor pixel 140 to be used in the present invention may be of any type, a detailed description of the image sensor pixel is omitted here.
  • the partition wall 120 distinguishes the cell culture unit 150, and the cells to be cultured are contacted, so that the partition wall 120 is formed of polymer or glass (glass, quartz, SiO 2 ) that does not cause a problem in contact with a living body. It is desirable to.
  • the partition wall 120 may be formed using the above materials in a general semiconductor manufacturing process, but may be formed in a heterojunction manner after the process is completed.
  • the number of the image sensor pixels 140 included in one observation unit 130 corresponding to one cell culture unit 150 may vary depending on the type of cells to be cultured and the area of the observation unit 130. have.
  • FIG. 1 for convenience of description, one cell culture unit 150 and one observation unit 130 corresponding thereto are illustrated, but various modifications are possible.
  • Figure 2 is another embodiment of the on-chip cell culture observation integrated unit according to the present invention.
  • the on-chip cell culture observation integrated unit 200 can be implemented in a structure in which a plurality of observations (131 ⁇ 134) is formed in one cell culture surrounded by the partition wall (120). have. In this case, it is natural that each of the observation units 131 to 134 should be able to control them individually.
  • Figure 3 is an embodiment of an on-chip cell culture observation integrated kit according to the present invention.
  • the on-chip cell culture observation integrated kit 300 includes a plurality of cell culture units surrounded by partition walls 121 to 124 and a plurality of observation units 131 to 134 formed under the cell culture units, respectively.
  • the on-chip cell culture observation integration kit 300 shown in FIG. 3 may be implemented by two-dimensionally arranging the on-chip cell culture observation integration unit 100 shown in FIG. 1. In this case, each cell culture section may be effective when one cell is cultured under different culture conditions.
  • Figure 4 is another embodiment of the on-chip cell culture observation integrated kit according to the present invention.
  • the on-chip cell culture observation integration kit 400 may be implemented by two-dimensional arrangement of the on-chip cell culture observation integration unit 200 shown in FIG.
  • This structure is effective for culturing several kinds of cells under the same culture conditions or for culturing one kind of cells under various culture conditions.
  • the pixels for the image sensor are omitted in the observation units 131 to 134.

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Abstract

The present invention relates to: an on-chip integrated unit for cell culture observation, wherein an incubator and observation equipment are implemented on one chip; and an on-chip integrated kit for cell culture observation. The on-chip integrated unit for cell culture observation comprises one observation portion, and one cell culture portion. The observation portion is formed on a surface of a substrate. The cell culture portion is formed at an upper portion of the observation portion to culture cells, and is surrounded by a partition wall of a predetermined height.

Description

온 칩 세포배양관찰 통합유닛 및 온 칩 세포배양관찰 통합키트 On-Chip Cell Culture Monitoring Integration Unit and On-Chip Cell Culture Monitoring Integration Kit
본 발명은 세포배양 및 배양된 세포를 관찰할 수 있는 세포배양관찰 장치에 관한 것으로, 특히, 배양된 세포를 관찰하는데 사용되는 관찰부와 상기 관찰부의 상부에 세포를 배양하는 세포배양부가 형성된 온 칩 세포배양관찰 통합유닛에 관한 것이다. The present invention relates to a cell culture observation apparatus capable of observing cell culture and cultured cells, and in particular, an on-chip cell having an observation part used for observing cultured cells and a cell culture part for culturing cells on the observation part. It relates to a culture observation integrated unit.
세포의 배양은 세포가 포함된 배지(culture plate)를 배양기(incubator)에 넣어 세포를 배양하는 세포 배양단계와 세포의 배양과정 및 분화 특성을 관찰하고 분석하는 모니터링 단계로 크게 구분할 수 있다. 세포 배양이 진행되는 배양기의 내부와 관찰이 진행되는 관찰 장비가 설치된 곳의 환경은 상당히 다르다. 따라서, 관찰도중 세포는 스트레스를 받거나 오염되어, 세포에 대한 특정 자극에 따른 변화를 관찰 할 때 오류가 발생하게 된다. Cell culture can be broadly classified into a cell culture step of culturing cells by placing a culture plate containing cells in an incubator and a monitoring step of observing and analyzing the culture process and differentiation characteristics of the cells. The environment inside the incubator where the cell culture proceeds and where the observation equipment where the observation proceeds is installed is quite different. Thus, during observation, the cells are stressed or contaminated, and an error occurs when observing changes caused by specific stimuli to the cells.
이러한 문제를 해결하기 위하여, 배양기와 관찰 장비가 하나로 결합한 통합시스템이 제안되었다. 세포배양 통합시스템은 관찰 장비를 배양기의 내부에 설치하는 것인데, 고성능의 관찰 장비는 그 부피가 크기 때문에, 정밀한 환경에서 운영되어야 하는 배양기의 규모를 크게 하는 문제점이 있다. 또한 고성능의 관찰 장비는 고가여서 세포관찰에 소요되는 경비가 증가하게 되는 문제가 있다. To solve this problem, an integrated system has been proposed in which the incubator and the observation equipment are combined into one. Cell culture integrated system is to install the observation equipment inside the incubator, because the high-performance observation equipment has a large volume, there is a problem to increase the size of the incubator to be operated in a precise environment. In addition, high-performance observation equipment is expensive, there is a problem that the cost required for cell observation increases.
본 발명이 해결하고자 하는 기술적 과제는, 배양기와 관찰장비를 하나의 칩에 구현한 온 칩 세포배양관찰 통합유닛을 제공하는 것에 있다. The technical problem to be solved by the present invention is to provide an on-chip cell culture observation integrated unit that implements the incubator and observation equipment in one chip.
본 발명이 해결하고자 하는 다른 기술적 과제는, 상기 온 칩 세포배양관찰 통합유닛을 이용하는 온 칩 세포배양관찰 통합키트를 제공하는 것에 있다. Another technical problem to be solved by the present invention is to provide an on-chip cell culture observation integration kit using the on-chip cell culture observation integration unit.
상기 기술적 과제를 달성하기 위한 본 발명의 일 실시예에 따른 온 칩 세포배양관찰 통합유닛은, 하나의 관찰부 및 하나의 세포배양부를 포함한다. 상기 관찰부는 기판의 표면에 형성된다. 상기 세포배양부는 상기 관찰부의 상부에 형성되어 세포를 배양하며, 일정한 높이의 격벽으로 둘러 쌓여 있다. On-chip cell culture observation integrated unit according to an embodiment of the present invention for achieving the above technical problem, includes one observation unit and one cell culture unit. The observation portion is formed on the surface of the substrate. The cell culture part is formed on the upper part of the observation part to culture the cells, and is surrounded by a partition of a constant height.
상기 기술적 과제를 달성하기 위한 본 발명의 다른 실시예에 따른 온 칩 세포배양관찰 통합유닛은, 하나의 관찰부 및 복수 개의 세포배양부를 포함한다. 상기 관찰부는 기판의 표면에 형성된다. 상기 세포배양부는 상기 관찰부의 상부에 형성되며 세포를 배양한다. 상기 복수 개의 세포배양부 전체는 일정한 높이의 단일 격벽으로 둘러 쌓여 있다. On-chip cell culture observation integrated unit according to another embodiment of the present invention for achieving the above technical problem, includes one observation unit and a plurality of cell culture unit. The observation portion is formed on the surface of the substrate. The cell culture unit is formed on the observation unit and cultures the cells. The plurality of cell culture parts are enclosed by a single partition wall having a constant height.
상기 기술적 과제를 달성하기 위한 본 발명의 또 다른 실시예에 따른 온 칩 세포배양관찰 통합유닛은, 복수 개의 관찰부 및 복수 개의 세포배양부를 포함한다. 상기 관찰부는 기판의 표면에 형성된다. 상기 세포배양부는 상기 복수 개의 관찰부의 상부에 각각 형성되며 세포를 배양한다. 상기 세포배양부 각각은 일정한 높이의 격벽으로 둘러 쌓여 있다.On-chip cell culture observation integrated unit according to another embodiment of the present invention for achieving the above technical problem, comprises a plurality of observation and a plurality of cell culture. The observation portion is formed on the surface of the substrate. The cell culture unit is formed on top of the plurality of observation units, respectively, to culture the cells. Each of the cell culture units is surrounded by a partition wall having a constant height.
상기 다른 기술적 과제를 달성하기 위한 본 발명에 따른 온 칩 세포배양관찰 통합키트는 청구항 제1항에 기재된 온 칩 세포배양관찰 통합유닛을 복수 개 포함한다. On-chip cell culture observation integration kit according to the present invention for achieving the above another technical problem comprises a plurality of on-chip cell culture observation integration unit according to claim 1.
본 발명에 따른 온 칩 세포배양관찰 통합유닛 및 온 칩 세포배양관찰 통합키트는, 센서에서 검출된 신호를 이용하여 센서의 표면에서 배양되는 세포의 성장단계와 분화특성을 실시간으로 용이하게 관찰할 수 있는 장점이 있다. The on-chip cell culture observation integrated unit and the on-chip cell culture observation integrated kit according to the present invention can easily observe the growth stage and differentiation characteristics of cells cultured on the surface of the sensor by using the signal detected by the sensor. There is an advantage.
또한 종래의 세포배양관찰 유닛에 비해 그 부피가 적기 때문에 종래의 배양기의 구조에도 다양하게 적용이 가능하다는 장점이 있다. In addition, since the volume is smaller than that of the conventional cell culture observation unit, there is an advantage that it can be variously applied to the structure of the conventional incubator.
도 1은 본 발명에 따른 온 칩 세포배양관찰 통합유닛의 일 실시 예이다. 1 is an embodiment of an on-chip cell culture observation integrated unit according to the present invention.
도 2는 본 발명에 따른 온 칩 세포배양관찰 통합유닛의 다른 일 실시 예이다. Figure 2 is another embodiment of the on-chip cell culture observation integrated unit according to the present invention.
도 3은 본 발명에 따른 온 칩 세포배양관찰 통합키트의 일 실시 예이다. Figure 3 is an embodiment of an on-chip cell culture observation integrated kit according to the present invention.
도 4는 본 발명에 따른 온 칩 세포배양관찰 통합키트의 다른 일 실시 예이다. Figure 4 is another embodiment of the on-chip cell culture observation integrated kit according to the present invention.
본 발명과 본 발명의 동작상의 이점 및 본 발명의 실시에 의하여 달성되는 목적을 충분히 이해하기 위해서는 본 발명의 예시적인 실시 예를 설명하는 첨부 도면 및 첨부 도면에 기재된 내용을 참조하여야만 한다. In order to fully understand the present invention, the operational advantages of the present invention, and the objects achieved by the practice of the present invention, reference should be made to the accompanying drawings that describe exemplary embodiments of the present invention and the contents described in the accompanying drawings.
이하 첨부한 도면을 참조하여 본 발명의 바람직한 실시 예를 설명함으로써, 본 발명을 상세히 설명한다. 각 도면에 제시된 동일한 참조부호는 동일한 부재를 나타낸다. Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. Like reference numerals in the drawings denote like elements.
도 1은 본 발명에 따른 온 칩 세포배양관찰 통합유닛의 일 실시 예이다. 1 is an embodiment of an on-chip cell culture observation integrated unit according to the present invention.
도 1의 상부에는 온 칩 세포배양관찰 통합유닛(100)의 상부에서 본 도면이 도시되어 있고, 도 1의 하부에는 온 칩 세포배양관찰 통합유닛(100)의 단면이 도시되어 있다. 본 발명에 따른 온 칩 세포배양관찰 통합유닛(100)은 관찰부(130) 및 세포배양부(150)를 포함한다. 관찰부(130)는 기판(110)의 상부에 형성되며 복수개의 이미지센서용 픽셀(140)을 포함한다. 세포배양부(150)는 관찰부(130)의 상부에 형성되며, 일정한 높이의 격벽(120)으로 둘러쌓여 있다. 1 is shown in the upper portion of the on-chip cell culture observation integrated unit 100, the upper portion of Figure 1 is shown in cross section of the on-chip cell culture observation integrated unit 100. On-chip cell culture observation integrated unit 100 according to the present invention includes an observation unit 130 and the cell culture unit 150. The observation unit 130 is formed on the substrate 110 and includes a plurality of image sensors pixels 140. The cell culture unit 150 is formed on the observation unit 130 and is surrounded by the partition wall 120 having a predetermined height.
각각의 이미지센서용 픽셀(140)에는 세포배양부(150)에서 배양된 세포를 통과하여 입사되는 빛에 대응되는 전하를 생성하는 포토다이오드(미도시) 및 포토다이오드로부터 생성된 전하에 대응되는 전기신호를 생성하는 변환회로를 포함한다. 본 발명에서 사용하고자 하는 이미지센서용 픽셀(140)은 어떠한 형태의 것이라도 관계없으므로, 여기서는 이미지센서용 픽셀의 구체적인 설명은 생략한다. Each image sensor pixel 140 includes a photodiode (not shown) for generating a charge corresponding to light incident through a cell cultured in the cell culture unit 150 and an electricity corresponding to a charge generated from the photodiode. And a conversion circuit for generating a signal. Since the image sensor pixel 140 to be used in the present invention may be of any type, a detailed description of the image sensor pixel is omitted here.
격벽(120)은 세포배양부(150)를 구별하는 것으로, 배양되는 세포가 접촉하게 되므로, 생체와의 접촉에도 문제가 발생하지 않는 폴리머(Polymer) 또는 유리질(glass, quartz, SiO2)로 구현하는 것이 바람직하다. 격벽(120)은 일반적인 반도체 제조 공정 중에 상기 재질을 이용하여 형성하는 것도 가능하지만, 공정이 종료된 후 이종접합방식으로 형성하는 것도 가능하다. The partition wall 120 distinguishes the cell culture unit 150, and the cells to be cultured are contacted, so that the partition wall 120 is formed of polymer or glass (glass, quartz, SiO 2 ) that does not cause a problem in contact with a living body. It is desirable to. The partition wall 120 may be formed using the above materials in a general semiconductor manufacturing process, but may be formed in a heterojunction manner after the process is completed.
당연한 설명이지만, 하나의 세포배양부(150)에 대응되는 하나의 관찰부(130)에 포함되는 이미지센서용 픽셀(140)의 개수는 배양되는 세포의 종류와 관찰부(130)의 면적에 따라 변할 수 있다. Naturally, the number of the image sensor pixels 140 included in one observation unit 130 corresponding to one cell culture unit 150 may vary depending on the type of cells to be cultured and the area of the observation unit 130. have.
도 1에는 설명의 편의를 위해, 하나의 세포배양부(150)와 이에 대응되는 하나의 관찰부(130)에 대하여 도시하였지만, 다양한 형태의 변형이 가능하다. In FIG. 1, for convenience of description, one cell culture unit 150 and one observation unit 130 corresponding thereto are illustrated, but various modifications are possible.
도 2는 본 발명에 따른 온 칩 세포배양관찰 통합유닛의 다른 일 실시 예이다. Figure 2 is another embodiment of the on-chip cell culture observation integrated unit according to the present invention.
도 2를 참조하면, 본 발명에 따른 온 칩 세포배양관찰 통합유닛(200)은 격벽(120)으로 둘러싸인 하나의 세포배양부에 복수 개의 관찰부(131 ~134)가 형성되어 있는 구조로도 구현할 수 있다. 이 경우 각각의 관찰부(131~134)는 개별적으로 제어를 할 수 있어야 함은 당연하다. 2, the on-chip cell culture observation integrated unit 200 according to the present invention can be implemented in a structure in which a plurality of observations (131 ~ 134) is formed in one cell culture surrounded by the partition wall (120). have. In this case, it is natural that each of the observation units 131 to 134 should be able to control them individually.
복잡함을 피하기 위해 도면에는 도시하지 않았지만, 하나의 관찰부에 복수 개의 세포배양부를 형성하도록 하는 구조도 가능하며, 이 구조도 본 발명의 권리범위에 포함될 것이다. Although not shown in the drawings to avoid the complexity, a structure for forming a plurality of cell cultures in one observation unit is also possible, and this structure will be included in the scope of the present invention.
도 3은 본 발명에 따른 온 칩 세포배양관찰 통합키트의 일 실시 예이다. Figure 3 is an embodiment of an on-chip cell culture observation integrated kit according to the present invention.
도 3을 참조하면, 온 칩 세포배양관찰 통합키트(300)는 각각 격벽(121~124)으로 둘러싸인 복수 개의 세포배양부 및 세포배양부의 하부에 형성된 복수 개의 관찰부(131~134)를 포함한다. 도 3에 도시된 온 칩 세포배양관찰 통합키트(300)는 도 1에 도시된 온 칩 세포배양관찰 통합유닛(100)을 2차원적으로 배열함으로써 구현할 수도 있다. 이 경우 각각의 세포배양부에는 한 종류의 세포를 각각 다른 배양조건으로 배양하는 경우에 효과적일 것이다. Referring to FIG. 3, the on-chip cell culture observation integrated kit 300 includes a plurality of cell culture units surrounded by partition walls 121 to 124 and a plurality of observation units 131 to 134 formed under the cell culture units, respectively. The on-chip cell culture observation integration kit 300 shown in FIG. 3 may be implemented by two-dimensionally arranging the on-chip cell culture observation integration unit 100 shown in FIG. 1. In this case, each cell culture section may be effective when one cell is cultured under different culture conditions.
도 4는 본 발명에 따른 온 칩 세포배양관찰 통합키트의 다른 일 실시 예이다. Figure 4 is another embodiment of the on-chip cell culture observation integrated kit according to the present invention.
도 4를 참조하면, 온 칩 세포배양관찰 통합키트(400)는 도 2에 도시된 온 칩 세포배양관찰 통합유닛(200)을 2차원적으로 배열함으로써 구현할 수도 있다. 4, the on-chip cell culture observation integration kit 400 may be implemented by two-dimensional arrangement of the on-chip cell culture observation integration unit 200 shown in FIG.
이 구조는 여러 종류의 세포를 동일한 배양 조건으로 배양하거나, 한 종류의 세포를 다양한 배양 조건으로 배양하는데 효과적이다. This structure is effective for culturing several kinds of cells under the same culture conditions or for culturing one kind of cells under various culture conditions.
도면의 복잡함을 피하기 위하여, 도 2 내지 도 4의 경우, 관찰부(131 ~134)에는 이미지센서용 픽셀이 생략되어 있다. In order to avoid the complexity of the drawing, in the case of FIGS. 2 to 4, the pixels for the image sensor are omitted in the observation units 131 to 134.
이상에서는 본 발명에 대한 기술사상을 첨부 도면과 함께 서술하였지만 이는 본 발명의 바람직한 실시 예를 예시적으로 설명한 것이지 본 발명을 한정하는 것은 아니다. 또한 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 이라면 누구나 본 발명의 기술적 사상의 범주를 이탈하지 않는 범위 내에서 다양한 변형 및 모방 가능함은 명백한 사실이다. In the above description, the technical idea of the present invention has been described with the accompanying drawings, which illustrate exemplary embodiments of the present invention by way of example and do not limit the present invention. In addition, it is obvious that any person having ordinary knowledge in the technical field to which the present invention belongs can make various modifications and imitations without departing from the scope of the technical idea of the present invention.

Claims (8)

  1. 기판의 표면에 형성된 하나의 관찰부; 및 One observation portion formed on the surface of the substrate; And
    상기 관찰부의 상부에 형성되며 세포를 배양하는 적어도 하나의 세포배양부;를 포함하며, Is formed on top of the observation portion and at least one cell culture for culturing cells; includes,
    상기 세포배양부는 일정한 높이의 격벽으로 둘러쌓여 있는 것을 특징으로 하는 온 칩 세포배양관찰 통합유닛.The cell culture unit integrated on-chip cell culture observation unit, characterized in that surrounded by a partition of a certain height.
  2. 기판의 표면에 형성된 관찰부; 및 An observation part formed on the surface of the substrate; And
    상기 관찰부의 상부에 형성되며 세포를 배양하는 복수 개의 세포배양부;를 포함하며, It is formed on top of the observation portion; a plurality of cell culture for culturing cells; includes,
    상기 복수 개의 세포배양부 전체는 일정한 높이의 단일 격벽으로 둘러 쌓여있는 것을 특징으로 하는 온 칩 세포배양관찰 통합유닛.The whole cell culture unit is integrated on-chip cell culture observation unit, characterized in that surrounded by a single partition of a constant height.
  3. 기판의 표면에 형성된 복수 개의 관찰부; 및 A plurality of observation parts formed on the surface of the substrate; And
    상기 복수 개의 관찰부의 상부에 각각 형성되며 세포를 배양하는 복수 개의 세포배양부;를 포함하며, And a plurality of cell culture units formed on top of the plurality of observation units and culturing cells, respectively.
    상기 복수 개의 세포배양부 각각은 일정한 높이의 단일 격벽으로 둘러 쌓여있는 것을 특징으로 하는 온 칩 세포배양관찰 통합유닛. Each of the plurality of cell culture unit is on-chip cell culture observation integrated unit, characterized in that surrounded by a single partition of a constant height.
  4. 제1항 내지 제3항 중 어느 하나의 항에 있어서, 상기 관찰부에는, The said observation part is in any one of Claims 1-3,
    복수 개의 이미지센서용 픽셀들이 배열되어 있는 것을 특징으로 하는 온 칩 세포배양관찰 통합유닛. On-chip cell culture observation integrated unit, characterized in that a plurality of pixels for the image sensor is arranged.
  5. 제4항에 있어서, 상기 이미지센서용 픽셀 각각에는, The method of claim 4, wherein each of the image sensor pixels,
    배양된 세포를 통과하여 입사되는 빛에 대응되는 전하를 생성하는 포토다이오드; 및 A photodiode generating a charge corresponding to light incident through the cultured cell; And
    상기 포토다이오드로부터 생성된 전하에 대응되는 전기신호를 생성하는 복수 개의 트랜지스터를 포함하는 변환회로;가 형성되어 있는 것을 특징으로 하는 온 칩 세포배양관찰 통합유닛. And a conversion circuit comprising a plurality of transistors for generating an electrical signal corresponding to the charge generated from the photodiode.
  6. 제5항에 있어서, 상기 격벽은, The method of claim 5, wherein the partition wall,
    생체에 적합한 폴리머 또는 유리질로 형성되는 것을 특징으로 하는 온 칩 세포배양관찰 통합유닛. On-chip cell culture observation integrated unit, characterized in that formed of a polymer or glassy material suitable for living organisms.
  7. 제1항 내지 제3항 중 어느 하나의 항에 기재된 온 칩 세포배양관찰 통합유닛을 복수 개 포함하는 온 칩 세포배양관찰 통합키트.An on-chip cell culture observation integration kit comprising a plurality of on-chip cell culture observation integration units according to any one of claims 1 to 3.
  8. 제7항에 있어서, 상기 격벽은, The method of claim 7, wherein the partition wall,
    생체에 적합한 폴리머 또는 유리질로 형성되는 것을 특징으로 하는 온 칩 세포배양관찰 통합키트.On-chip cell culture observation integrated kit, characterized in that formed of a polymer or glassy material suitable for the living body.
PCT/KR2013/010347 2012-11-16 2013-11-14 On-chip integrated unit for cell culture observation, and on-chip integrated kit for cell culture observation WO2014077601A1 (en)

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JP2003329681A (en) * 2002-05-14 2003-11-19 Hitachi Ltd Biological sample testing apparatus
KR20080059593A (en) * 2005-09-30 2008-06-30 셀룰라 바이오엔지니어링 인코포레이티드 Apparatus and method for detecting activity of living cells
JP2008237064A (en) * 2007-03-26 2008-10-09 Tsuru Gakuen Apparatus for observing cell and method for observing cell
KR20100136666A (en) * 2009-06-19 2010-12-29 전자부품연구원 Cell observation apparatus

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003329681A (en) * 2002-05-14 2003-11-19 Hitachi Ltd Biological sample testing apparatus
KR20080059593A (en) * 2005-09-30 2008-06-30 셀룰라 바이오엔지니어링 인코포레이티드 Apparatus and method for detecting activity of living cells
JP2008237064A (en) * 2007-03-26 2008-10-09 Tsuru Gakuen Apparatus for observing cell and method for observing cell
KR20100136666A (en) * 2009-06-19 2010-12-29 전자부품연구원 Cell observation apparatus

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