WO2014076232A3 - Isopropanol production by recombinant hosts using an hmg-coa intermediate - Google Patents

Isopropanol production by recombinant hosts using an hmg-coa intermediate Download PDF

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Publication number
WO2014076232A3
WO2014076232A3 PCT/EP2013/073933 EP2013073933W WO2014076232A3 WO 2014076232 A3 WO2014076232 A3 WO 2014076232A3 EP 2013073933 W EP2013073933 W EP 2013073933W WO 2014076232 A3 WO2014076232 A3 WO 2014076232A3
Authority
WO
WIPO (PCT)
Prior art keywords
hmg
recombinant hosts
coa intermediate
isopropanol production
isopropanol
Prior art date
Application number
PCT/EP2013/073933
Other languages
French (fr)
Other versions
WO2014076232A2 (en
Inventor
Bjarke Christensen
Peter Bjarke Olsen
Original Assignee
Novozymes A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes A/S filed Critical Novozymes A/S
Publication of WO2014076232A2 publication Critical patent/WO2014076232A2/en
Publication of WO2014076232A3 publication Critical patent/WO2014076232A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Provided herein are recombinant host cells having an active isopropanol pathway including one or both of a first heterologous polynucleotide encoding an HMG-CoA synthase and a second heterologous polynucleotide encoding an HMG-CoA lyase. Also described are methods of using the recombinant host cells to produce isopropanol and propylene.
PCT/EP2013/073933 2012-11-19 2013-11-15 Isopropanol production by recombinant hosts using an hmg-coa intermediate WO2014076232A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261727876P 2012-11-19 2012-11-19
US61/727,876 2012-11-19

Publications (2)

Publication Number Publication Date
WO2014076232A2 WO2014076232A2 (en) 2014-05-22
WO2014076232A3 true WO2014076232A3 (en) 2014-08-14

Family

ID=49641730

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2013/073933 WO2014076232A2 (en) 2012-11-19 2013-11-15 Isopropanol production by recombinant hosts using an hmg-coa intermediate

Country Status (1)

Country Link
WO (1) WO2014076232A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112021012231A2 (en) 2018-12-28 2021-09-28 Braskem S.A. MODULATION OF CARBON FLOW THROUGH MEG AND C3 PATHWAYS FOR IMPROVED PRODUCTION OF MONOETHYLENE GLYCOL AND C3 COMPOUNDS
EP4085146A1 (en) * 2020-02-21 2022-11-09 Braskem, S.A. Production of ethanol with one or more co-products in yeast
CN111304105B (en) * 2020-02-27 2022-05-03 南京工业大学 Genetically engineered bacterium for producing lipase by using methanol and xylose cosubstrate and application thereof

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JP5121070B2 (en) 2005-11-23 2013-01-16 ネイチャーワークス・エル・エル・シー L- or D-lactate: Lactic acid-producing yeast in which ferricytochrome C oxidoreductase gene does not function
JP5443970B2 (en) 2006-03-13 2014-03-19 カーギル インコーポレイテッド Yeast cells with disrupted pathway from dihydroxyacetone phosphate to glycerol
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Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
WO2007140339A2 (en) * 2006-05-26 2007-12-06 Amyris Biotechnologies, Inc. Production of isoprenoids
WO2009076676A2 (en) * 2007-12-13 2009-06-18 Danisco Us Inc. Compositions and methods for producing isoprene
WO2011022651A1 (en) * 2009-08-21 2011-02-24 John Mcbride Production of propanols, alcohols, and polyols in consolidated bioprocessing organisms
WO2012058603A1 (en) * 2010-10-29 2012-05-03 Novozymes A/S Recombinant n-propanol and isopropanol production

Also Published As

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