WO2014040380A1 - 复合菌群及其应用 - Google Patents
复合菌群及其应用 Download PDFInfo
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- WO2014040380A1 WO2014040380A1 PCT/CN2013/001081 CN2013001081W WO2014040380A1 WO 2014040380 A1 WO2014040380 A1 WO 2014040380A1 CN 2013001081 W CN2013001081 W CN 2013001081W WO 2014040380 A1 WO2014040380 A1 WO 2014040380A1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/90—Apparatus therefor
- C05F17/964—Constructional parts, e.g. floors, covers or doors
- C05F17/971—Constructional parts, e.g. floors, covers or doors for feeding or discharging materials to be treated; for feeding or discharging other material
- C05F17/986—Constructional parts, e.g. floors, covers or doors for feeding or discharging materials to be treated; for feeding or discharging other material the other material being liquid
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- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- C05F7/00—Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
- C05F7/005—Waste water from industrial processing material neither of agricultural nor of animal origin
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- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
- D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C3/00—Pulping cellulose-containing materials
- D21C3/22—Other features of pulping processes
- D21C3/26—Multistage processes
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
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- C12R2001/38—Pseudomonas
- C12R2001/39—Pseudomonas fluorescens
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- C12R2001/85—Saccharomyces
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Definitions
- the invention relates to a composite flora and its application in preparing textile fibers, cellulose for additives and pulping of biological bacteria liquid.
- hemp fiber has been favored by consumers because of its ecological and environmental protection characteristics. Its demand is increasing year by year, and the global natural fiber growth rate is 8% per year.
- the main characteristics of hemp fiber raw materials are high fiber content, slender fiber is conducive to interweaving, and good strength; small cell cavity, thick cell wall, and large wall-to-cavity ratio; due to the fine cell and fine fiber of hemp fiber, the opacity is high.
- the disadvantage is that the fiber is not easy to be separated, so that the book
- the finished fabric has a low air permeability.
- the fiber is prepared from the hemp raw material, and the waste liquid generated by the chemical method and the chemical method is polluted, destroys the land, pollutes the air, and has high energy consumption, power consumption and large water consumption.
- the waste liquid generated in chemical pulp production pollutes the environment, destroys the land and pollutes the air.
- Waste liquid discharge is large; waste that cannot be reused can only be discharged to the outside. Does not comply with national energy conservation and emission reduction policies. Substances cannot be effectively recycled. Chemical preparations cannot be separated from waste liquids, organic substances are mixed with chemical preparations, and organic substances cannot be reused, resulting in a large loss.
- bio-fiber technology it is necessary to develop bio-fiber technology to fundamentally solve the above-mentioned pollution problems, save energy and reduce emissions, save water, reduce production costs and increase the use rate of materials.
- An object of the present invention is to overcome the above-mentioned drawbacks of the prior art chemical fiber-making fibers and to provide a novel biological bacteria for preparing fibers.
- the inventors obtained a biological bacterium suitable for the production of fibers capable of achieving the above object by a long screening operation.
- the present invention provides a composite flora comprising Bacillus sp. with the accession number CGMCC No. 5971 and Einheimem tangshanensis deposited under the accession number CGMCC No. 5972, the accession number is CcineCC No. 5973 Acinetobacter Iwoffi, Pseudomonas fluorescens with CGMCC No. 5974, Wickerhamomyces anomalus with CGMCC No. 5975 (Wickerhamomyces anomalus in Wort Liquid Culture) The cells were cultured at 25 °C for three days. The cells were spherical, ovate, and sausage-shaped.
- the size was (4.8-14.4) X (3.6-7.2) ⁇ , and a precipitate formed.
- the malt agar slant was cultured at 25 °C for one month, and the colony cheese Shaped, milky white Color, smooth surface, non-reflective, edge-rooted. Corn flour agar Dalmau plate culture, producing pseudohyphae).
- the composite flora can be used for preparing textile fibers, cellulose for additives, and biological bacterial liquid pulping.
- the invention also provides a method for preparing textile fibers, which mainly comprises the steps of:
- the above-mentioned composite bacterial group is configured as a mixed bacterial aqueous solution according to the following mass ratio, that is, the bacterial liquid:
- R. jejuni Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus 2-3 : 1-2 : 1-2 : 1-2 : 2-3;
- Raw material processing cutting the hemp raw material into segments, and swelling the raw materials into the soaking pool; preferably, the hemp raw materials are flax, ramie, yellow kenaf or sisal;
- Biodegradation Soak the raw material after dissolving into the configured bacterial liquid
- the biodegradable raw materials are removed from the bacterial liquid, drained, and steamed; the fiber is obtained: the sterilized raw material is subjected to coarse grinding to form a fiber bundle; Two-stage fine grinding to disperse the fiber bundle into a single fiber; screening and filtering the fiber bundle in the slurry after a rough grinding and two fine grinding, and re-pulping to make a single fiber;
- Drying, carding The fibers obtained above are soaked in warm water, then dried and carded for the preparation of textile fibers.
- the invention also provides a method for preparing cellulose for additives, which mainly comprises the steps of:
- the above-mentioned composite bacterial group is configured as a mixed bacterial aqueous solution according to the following mass ratio, that is, the bacterial liquid:
- R. jejuni Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus 2-3 : 1-2 : 1-2 : 1-2 : 2-3;
- Raw material processing After peeling the woody raw materials, or cutting the herbal raw materials into segments, the cut raw materials are placed in the soaking pool to swell;
- Disintegration squeezing the swollen raw material and/or squeezing
- Biodegradation Soak the raw material after dissolving into the configured bacterial liquid
- the biodegradable raw materials are removed from the bacterial liquid, drained, and steamed; the fiber is obtained: the sterilized raw material is subjected to coarse grinding to form a fiber bundle; Two-stage fine grinding to disperse the fiber bundle into a single fiber; screening and filtering the fiber bundle in the slurry after a rough grinding and two fine grinding, and re-pulping to make a single fiber;
- Sterilization Soak the fiber prepared above in warm water, then dry and sterilize;
- the sterilized fibers are ground into cellulose as an additive.
- the invention also provides a biological bacterial liquid pulping method, which mainly comprises the steps of: 1) Disposition of the bacterial liquid:
- the above-mentioned composite bacterial group is configured as a mixed bacterial aqueous solution according to the following mass ratio, that is, the bacterial liquid:
- R. jejuni Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus 1-3: 1-3: 1-2: 1-2: 2-3;
- Raw material processing After peeling or cutting the woody raw materials, or cutting the herbal raw materials into sections, the cut raw materials are put into the soaking pool to swell;
- Disintegration squeezing the swollen raw material and/or squeezing
- Biodegradation Soak the raw material after dissolving into the configured bacterial liquid
- Fine refining The above-mentioned one-stage coarse refining is subjected to two-stage fine refining to disperse the fiber bundle into a single fiber; slurry screening: screening, filtering through a slurry after a coarse refining and two-stage fine refining The fiber bundle is repulped and made into a single fiber;
- Submersible, washing The pulp obtained above is soaked in warm water and used for making cardboard.
- the density of the bacterial liquid formed in the step 1) is 60 million / ml or more.
- the swelling time is 10-12 h.
- Step 3) The biodegradation temperature is maintained at 35-40 ° C for 28-32 hours.
- the mass ratio of the raw material to the bacterial liquid after the disintegration is 1: 6-9.
- the steam sterilization is sterilized by atmospheric pressure steam for 10-30 minutes.
- step 2) the soaking liquid after soaking the raw material is flocculated and precipitated, and the supernatant is recovered and reused, and the precipitate is input into the biogas tank to be fermented to generate biogas.
- the biological method provided by the invention has the advantages of: 1) no pollution to the environment: the waste liquid is directly converted into an organic fertilizer, achieving zero discharge and zero pollution. 2)
- the biological method can protect the fiber. Compared with the traditional chemical method, the method can recover both the whole fiber and the half fiber, thereby increasing the yield. 3) Biological methods are degraded under normal pressure, energy saving, emission reduction, and low carbon. 4) Low production costs and high economic returns.
- the by-product of the present invention is sent to a sedimentation tank for flocculation and sedimentation, and the supernatant liquid is returned to secondary use, and then used as a pre-dip liquid.
- the floc is rich in a variety of organic matter and phytonutrients such as N, P, K, and the floc is mixed with the old bacterial liquid (multiple degradation of the raw viscous liquid, also containing N, P, K, Fe and trace elements). , acidified, and then discharged into the biogas fermentation tank to produce biogas.
- the biogas residue, the biogas slurry and the pulverized boiler ash are mixed and granulated to form a granular organic fertilizer, and finally discharged to the factory to achieve zero discharge.
- the present invention further improves the existing techniques for preparing cellulose and biopulping by providing the above-mentioned biological bacteria obtained by the inventor after a long period of creative labor, which reduces the reaction time and improves the purity and yield of the obtained fiber.
- This enables the technology to be widely applied in actual production.
- the application of the biological bacteria degrades the plant to obtain the fiber in a short time, and the biological bacteria degrade the lignin in the plant body to produce pulp and paper in a short time, and the by-product is converted into biogas twice, and the biogas is supplied to the coal and gas boiler for combustion and heating. Save coal consumption.
- biogas residue system As an organic fertilizer, it has formed a new economic cycle of “substance organic transformation”, achieving no waste discharge, that is, zero emissions.
- the problem of pollution of chemically prepared fibers in the prior art is fundamentally solved. Energy saving and emission reduction, saving water, reducing production costs and increasing the use rate of materials.
- FIG. 1 is a flow chart of preparing textile fibers in accordance with an embodiment of the present invention
- FIG. 2 is a flow chart of preparing cellulose for an additive according to an embodiment of the present invention.
- FIG. 3 is a flow chart of preparing cellulose for an additive according to another embodiment of the present invention.
- FIG. 4 is a flow chart of a biological bacterial liquid pulping method according to an embodiment of the present invention.
- Figure 5 is a flow chart of a biological bacterial liquid pulping process in accordance with another embodiment of the present invention.
- the biological bacteria used in the present invention was deposited on April 6, 2012 at the General Microbiology Center of the China Microbial Culture Collection Management Committee (CGMCC, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing), which contains the deposit number CGMCC. No. 5971 Bacillus iBacillus sp ), CGMCC No. 5972, Rheinheimera tangshane is, CGMCC No. 5973, Acinetobacter IwoffiD, deposited as CGMCC No. Pseudomonas fluorescens 5974
- the above complex bacteria group is arranged as a mixed bacteria aqueous solution according to the following mass ratio, that is, the bacterial liquid:
- the composite flora was configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus was 3:1:1:2:3.
- the flow of the fiber preparation method is divided into three stages: preparation stage, fiber section and by-product section.
- Biodegradation The decomposed raw materials are input into the biological bacteria degradation tank or tank, and immersed in the bacterial liquid prepared in Example 1. The mass ratio of the decomposed raw materials to the bacterial liquid is 1:8, and the temperature is maintained at 35- 40 ° C, time 28-32 hours. The degradation reaction occurs under the conditions of the biological bacteria, and its specific effect is exerted.
- Re-screening Screening and filtering the fiber bundles in a slurry after a coarse grinding and a second-stage fine grinding, and re-pulping them to make a single fiber.
- the residue of the raw materials separated in A and 3 is rich in nutrients, and is fermented and converted into cattle and sheep.
- the liquid After immersing and washing the liquid in B and 4, the liquid is turbid, and after flocculation and sedimentation, the supernatant is recovered and reused.
- the sediment is input into the biogas tank to produce biogas, which is fed into the gas dual-purpose boiler for fuel, which can save energy. Consumption.
- Bio-organic fertilizer The biogas residue and biogas slurry fermented by the biogas tank are rich in bio-organic fertilizer.
- the liquid is used as crop topdressing and flower nutrient solution, and solid granulation is used as base fertilizer, which is green fertilizer.
- the specific preparation process is the same as in Example 2.
- the difference is that the composite flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus is 3:1:2:2:2; In the case of biodegradation, the mass ratio of the raw material to the bacterial liquid after the disintegration is 1:7.
- the specific preparation process is the same as in Example 2.
- the difference is that the composite flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus is 3:2:1:2:2; In the case of biodegradation, the mass ratio of the raw material to the bacterial liquid after the disintegration is 1:8.5.
- the specific preparation process is the same as in Example 2.
- the difference is that the composite flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2:1:2:2:3; In the case of biodegradation, the mass ratio of the raw material to the bacterial liquid after the disintegration is 1:9.
- Caragana korshinskii as raw material, the preparation method of cellulose when using woody raw materials is specifically described.
- the preparation process of the remaining woody materials, such as poplar and willow, can be carried out by referring to the process.
- the complex flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens:
- Wickerhamomyces anomalus is 3:1:2:2:2; when poplar is used, the complex flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces ano Painting his is 2 ⁇ :2:2 ⁇ 3 ⁇ When using willow, the complex flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalu is 3:2: 1 :2:2.
- the above four steps can be intermittent or interlocking.
- Disintegration The swollen branches are fed into a silk reeling machine or squeezed and smashed to modify the wood structure to loosen into a wood-like shape, which is beneficial to the penetration of biological bacteria and exerts its degradation effect.
- Biodegradation The decomposed raw materials are input into the biological bacteria degradation tank or tank, and immersed in the bacterial liquid prepared in Example 1.
- the mass ratio of the decomposed raw materials to the bacterial liquid is 1:6, and the temperature is maintained at 35- 40 ° C, time 28-32 hours.
- the lignin reaction is degraded under the conditions of biological bacteria, and its specific effect is exerted.
- the sterilized raw material is subjected to a rough grinding to form a fiber bundle; the above-mentioned one-stage coarse grinding is subjected to two-stage fine grinding to disperse the fiber bundle into a single fiber; screening, filtering through a rough grinding and two The fiber bundle in the finely ground slurry is reground to make a single fiber.
- the caragana peel isolated from A 3 is rich in nutrients and fermented into cattle and sheep.
- the flocculation and sedimentation are carried out, and the supernatant is recovered and reused.
- the sediment is input into the biogas digester to produce biogas, and the gas is used as a dual-purpose boiler to make fuel, which can save energy and reduce consumption. effect.
- Bio-organic fertilizer The biogas residue and biogas slurry fermented by the biogas tank are rich in bio-organic fertilizer.
- the liquid is used as crop topdressing and flower nutrient solution, and solid granulation is used as base fertilizer, which is green fertilizer.
- the wheat straw is used as a raw material, and the preparation method of the cellulose when the herbal raw material is used is specifically described.
- the preparation process of the remaining herbal materials, such as straw and reed, can be carried out by referring to the process.
- the composite flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus is 3:2:1:2:2; When straw is used, the composite flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2:2:2:2:2; In the case of reed, the complex flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter rufii: Fluorescent pseudomonas, please refer to Figure 3, the process of cellulose preparation is divided into three stages. : Preparation stage, cellulose section and by-product section.
- Preparation stage 1 4 Cut the wheat straw into 4-5cm cut sections, input into the soaking tank or soaking tank for washing and cold soaking. First, wash away the foreign materials such as the outer surface of the raw materials, and soak them at the same time. The water temperature is natural temperature, and the time is soaked and swelled. The 10-12ho liquid turbid after repeated soaking, after flocculation and precipitation, the supernatant can be reused. The sediment is fed into a biogas tank to produce biogas.
- the biological bacterial liquid pulping method when using woody raw materials is specifically described.
- the biopulping process of the remaining woody materials can be carried out with reference to the process.
- the complex flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus is 3: 1 : 2:2:2
- the complex flora is configured according to the following mass ratio: Bacillus: R.
- Acinetobacter ruta Pseudomonas fluorescens: Wickerhamomyces ano painted his 3 ⁇ .2 ⁇ ⁇ : 2: 2 ;
- the complex flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalu is 2:2: 1 :2:3
- the flow of the biological bacterial slurry method is divided into three stages: preparation stage, pulping section and by-product section.
- Disintegration The swollen branches are fed into a silk reeling machine or squeezed and smashed to modify the wood structure to loosen into a wood-like shape, which is beneficial to the penetration of biological bacteria and exerts its degradation effect.
- Biodegradation The decomposed raw materials are input into the biological bacteria degradation tank or tank, and immersed in the bacterial liquid prepared in the first embodiment.
- the mass ratio of the raw materials to the bacterial liquid after the disintegration is 1:6, and the temperature is maintained at 35- 40 ° C, time 28-32 hours.
- the lignin reaction is degraded under the conditions of biological bacteria, and its specific effect is exerted.
- Fine refining From the above-mentioned crude slurry, it is then transferred to a high-concentration refiner for fine refining to disperse the fiber bundle into a single fiber.
- Slurry screening After two stages of refining, the slurry contains a small number of fiber bundles, which are screened, filtered, and re-slurry to reach a single fiber.
- Submersible, washing The pulp after coarse refining and fine refining is mechanically rubbed, most of which is bent and twisted. It is soaked in warm water to eliminate the fiber deflection caused by refining and stretch it.
- Paperboard The paper pulp prepared above is fed into a paper machine, and paperboard is produced by a papermaking process.
- the caragana peels separated in A and 3 are rich in nutrients, and fermented into cattle and sheep.
- the liquid After immersing and washing the liquid in B and 4, the liquid is turbid, and after flocculation and sedimentation, the supernatant is recovered and reused.
- the sediment is input into the biogas tank to produce biogas, which is fed into the gas dual-purpose boiler for fuel, which can save energy. Consumption.
- Bio-organic fertilizer The biogas residue and biogas slurry fermented by the biogas tank are rich in bio-organic fertilizer.
- the liquid is used as crop topdressing and flower nutrient solution, and solid granulation is used as base fertilizer, which is green fertilizer.
- the biological bacterial liquid pulping method when using herbal raw materials is specifically described.
- the biopulping of the remaining herbal materials, such as wheat straw, straw, and corn stalk, can be carried out with reference to the process.
- the composite flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2:2:1:2:3; When straw is used, the complex flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens: Wickerhamomyces anomalus 1:3:1:2:3; In the case of corn stalks, the composite flora is configured according to the following mass ratio: Bacillus: R. jejuni: Acinetobacter rufii: Fluorescent false singles, please refer to Figure 5, the biological bacterial liquid pulping process is divided into three Stages: preparation stage, pulping section and by-product section.
- the tightness is not less than g/cm 3 AA 0.53 0.50 0.45
- Lateral ring pressure index is not less than
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US14/428,961 US20150232803A1 (en) | 2012-09-17 | 2013-09-16 | Bacillus sp. CGMCC#5971 and Its Application Thereof |
CA2885522A CA2885522C (en) | 2012-09-17 | 2013-09-16 | Complex microbial flora and application thereof |
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CN201210341453.1 | 2012-09-17 | ||
CN201210341453.1A CN103074247B (zh) | 2012-09-17 | 2012-09-17 | 复合菌群及用其制备纺织纤维的方法 |
CN2012103414851A CN102888338B (zh) | 2012-09-17 | 2012-09-17 | 生物菌群及用其制备添加剂用纤维素的方法 |
CN201210341519.7A CN102888339B (zh) | 2012-09-17 | 2012-09-17 | 生物菌群及生物菌液制浆法 |
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CN101838613A (zh) * | 2009-09-30 | 2010-09-22 | 河南省恒隆态生物工程股份有限公司 | 一种快速降解农作物秸秆的腐熟剂菌种组合 |
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2013
- 2013-09-16 WO PCT/CN2013/001081 patent/WO2014040380A1/zh active Application Filing
- 2013-09-16 US US14/428,961 patent/US20150232803A1/en not_active Abandoned
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CN101838613A (zh) * | 2009-09-30 | 2010-09-22 | 河南省恒隆态生物工程股份有限公司 | 一种快速降解农作物秸秆的腐熟剂菌种组合 |
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