WO2014004670A3 - Electrochemical system and method thereof - Google Patents
Electrochemical system and method thereof Download PDFInfo
- Publication number
- WO2014004670A3 WO2014004670A3 PCT/US2013/047888 US2013047888W WO2014004670A3 WO 2014004670 A3 WO2014004670 A3 WO 2014004670A3 US 2013047888 W US2013047888 W US 2013047888W WO 2014004670 A3 WO2014004670 A3 WO 2014004670A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- oxidoreductase
- working electrode
- interface
- provides
- voltage generator
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
An embodiment of the invention provides an ultrasensitive and selective system and method for detecting reactants of the chemical/biochemical reaction catalyzed by an oxidoreductase, such as glucose and ethanol, at a concentration level down to zepto molar (10-21 M). In embodiments, the invention provides an ampyometric immuno-sensing system comprising a working electrode, an oxidoreductase, and an external voltage generator, wherein the oxidoreductase is immobilized on the working electrode; and the voltage generator generates a voltage to induce an electric field that permeates at least a portion of the interface between the oxidoreductase and the working electrode. The ultrasensitivity of the system and method is believed to be caused by the electrical field, which enhances the quantum mechanical tunneling effect in the interface, and therefore facilitates the interfacial electron transfer between the oxidoreductase and the working electrode.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13/533,248 | 2012-06-26 | ||
US13/533,248 US9605295B2 (en) | 2007-11-07 | 2012-06-26 | Electrochemical system and method thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2014004670A2 WO2014004670A2 (en) | 2014-01-03 |
WO2014004670A3 true WO2014004670A3 (en) | 2014-04-10 |
Family
ID=48986203
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2013/047888 WO2014004670A2 (en) | 2012-06-26 | 2013-06-26 | Electrochemical system and method thereof |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2014004670A2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104764774B (en) * | 2015-01-22 | 2018-01-30 | 济南大学 | A kind of biology sensor for detecting Escherichia coli and preparation method thereof |
CN108896636B (en) * | 2018-05-14 | 2019-12-31 | 济南大学 | Preparation of superoxide dismutase modified vanadium nitride paste electrode sensor |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009062099A1 (en) * | 2007-11-07 | 2009-05-14 | Cleveland State University | Electrochemical system and method thereof |
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2013
- 2013-06-26 WO PCT/US2013/047888 patent/WO2014004670A2/en active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009062099A1 (en) * | 2007-11-07 | 2009-05-14 | Cleveland State University | Electrochemical system and method thereof |
Non-Patent Citations (5)
Title |
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CUIXIA MA ET AL: "Label-free sandwich type of immunosensor for hepatitis C virus core antigen based on the use of gold nanoparticles on a nanostructured metal oxide surface", MICROCHIMICA ACTA ; AN INTERNATIONAL JOURNAL ON MICRO AND TRACEANALYSIS, SPRINGER-VERLAG, VI, vol. 178, no. 3 - 4, 9 June 2012 (2012-06-09), pages 331 - 340, XP035097845, ISSN: 1436-5073, DOI: 10.1007/S00604-012-0842-1 * |
GAUTHAM KUMAR AHIRWAL ET AL: "Gold nanoparticles based sandwich electrochemical immunosensor", BIOSENSORS AND BIOELECTRONICS, vol. 25, no. 9, 15 May 2010 (2010-05-15), pages 2016 - 2020, XP055082548, ISSN: 0956-5663, DOI: 10.1016/j.bios.2010.01.029 * |
JIAPENG WANG ET AL: "Field-effect amperometric immuno-detection of protein biomarker", BIOSENSORS AND BIOELECTRONICS, ELSEVIER BV, NL, vol. 29, no. 1, 28 July 2011 (2011-07-28), pages 210 - 214, XP028297841, ISSN: 0956-5663, [retrieved on 20110804], DOI: 10.1016/J.BIOS.2011.07.072 * |
MILLIGAN CHRISTINE ET AL: "Laccase based sandwich scheme immunosensor employing mediatorless electrocatalysis", ELECTROANALYSIS, vol. 14, no. 6, April 2002 (2002-04-01), pages 415 - 419, XP002714249, ISSN: 1040-0397 * |
XIN YU ET AL: "Protein immunosensor using single-wall carbon nanotube forests with electrochemical detection of enzyme labels", MOLECULAR BIOSYSTEMS, vol. 1, no. 1, 1 January 2005 (2005-01-01), pages 70, XP055082717, ISSN: 1742-206X, DOI: 10.1039/b502124c * |
Also Published As
Publication number | Publication date |
---|---|
WO2014004670A2 (en) | 2014-01-03 |
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