Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/60—Sugars; Derivatives thereof
  • A61K8/602—Glycosides, e.g. rutin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/34—Alcohols
  • A61K8/345—Alcohols containing more than one hydroxy group
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/60—Sugars; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
  • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
  • A61K8/9783—Angiosperms [Magnoliophyta]
  • A61K8/9789—Magnoliopsida [dicotyledons]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
  • A61Q19/007—Preparations for dry skin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q5/00—Preparations for care of the hair

Definitions

• the invention relates to the field of cosmetics.
• the subject of the present invention is an extract of cotton fibers comprising a cotton fiber hydrolyzate consisting of mono-, di-, tri- and tetra-saccharides, and a cosmetic composition comprising, as active principle, in a physiologically suitable medium, at least one extract of cotton fibers.
• the skin is the major keratinous substrate of the body. It is a vital organ covering the entire surface of the body and providing multiple functions such as sensitive functions, protective against multiple external attacks, immune, metabolic or thermoregulatory. These roles are made possible by a complex structure that combines various tissue structures.
• the skin consists of three distinct compartments superimposed: the epidermis, the dermis and the hypodermis.
• the epidermis is a coating epithelium that constitutes the outer structure of the skin and provides its protective function. This function is ensured by the cohesion of the epithelial cells and by the production of a filamentous and resistant protein: keratin.
• the epidermis is characterized by an organization in strata corresponding to a state of increasing differentiation of keratinocytes, from the deepest zone (basal stratum) to the most superficial zone (stratum corneum). During their migration to the surface, the keratinocytes flatten out and the synthesis of keratin, a family of fibrous proteins, intensifies.
• the keratinocytes flatten again, their nucleus begins to degenerate.
• the cellular content rich in lipids, cholesterol, saturated free fatty acids and ceramides is then excreted in the extracellular space, which increases the cohesion between the cells and contributes to the role of barrier of the epidermis.
• the horny layer (stratum corneum) is thus very resistant to external aggressions.
• Keratin is the essential component of all keratin substrates and in particular hair fibers, hair, nails and other integuments.
• the hair is a capillary structure consisting of dead cells filled with keratin filaments and lipid residues from the plasma membranes.
• Each hair is produced by a hair follicle, itself consisting of a bulb, a hair shaft and a sebaceous gland.
• the bulb it is the keratinocytes located on the periphery that constitute the proliferative base. During differentiation, the keratinocytes move towards the center of the follicle to form the hair shaft and load in keratin fibers, which makes the hair very resistant.
• the technical problem to be solved was therefore, for the inventors, to find a new natural product of plant origin, physiologically acceptable, which is capable of providing real care for keratin substrates but also to protect the skin and hair of an effective way so that they do not undergo the damage caused by the aggressions and the stresses of external origin.
• the inventors have succeeded in selecting a natural product of plant origin which, when treated in an appropriate manner, presents remarkable properties when it is applied to a keratinous substrate.
• a cotton fiber extract comprising a cotton fiber hydrolyzate consisting exclusively of mono-, di-, tri- and tetrasaccharides has remarkable properties on keratin substrates and, in particular, that protects the skin and hair.
• Cotton is the whole of the fibers (or hairs) covering the cotton seed.
• the cotton plant, or Gossypium is a dicotyledon of the family Malvaceae. There are 40 to 50 perennial or annual species, woody or herbaceous. Only four of them are grown for their fiber: Gossypium hirsutum, Gossypium barbadense, Gossypium herbaceum and Gossypium arboreum. These four cotton species gave birth to many hybrid varieties, classified according to the length of their fiber, the pubescence of the seed and the shape of the bracts. It is the cotton line Gossypium hirsutum, which alone provides 80 to 90% of the world cotton production.
• the fruit of the cotton is an oval capsule, leathery, and containing many seeds bearing tight hairs (or fibers) of various lengths. It is from these fibers, appropriately treated, that the inventors have developed the new cotton fiber extract and the cosmetic composition according to the invention.
• the patent FR2799647 discloses compositions in the form of emulsion containing fibers, at least one silicone surfactant and at least one wax, for treating, protecting, treating, removing make-up and / or cleaning the skin, the lips and / or the hair.
• Cotton fibers are cited without being exemplified or preferred.
• the preferred fibers are synthetic and have a length of between 0.1 and 1.5 mm.
• compositions based on cotton honeydew in the patent application WO2004052331 for the care of keratinous substrates, said cotton honeydew being a substance secreted by insects and present on cotton. It contains mainly sugars synthesized by insects and sugars of physiological origin, including glucose, fructose, sucrose, trehalose, trehalulose, melezitoze and inositol.
• the present invention relates to a cotton fiber extract comprising a cotton fiber hydrolyzate consisting exclusively of monosaccharides and / or disaccharides and / or trisaccharides and / or tetrasaccharides.
• the term "cotton fiber hydrolyzate" according to the present invention means the product resulting from the hydrolysis of cotton fibers via an enzymatic action, in particular a cellulase.
• cotton fiber extract an aqueous solution comprising an enzymatically hydrolyzed cotton fiber hydrolyzate produced according to the method described hereinafter.
• saccharides are a class of organic molecules containing a carbonyl group (aldehyde or ketone) and several hydroxyl groups (-OH). Particularly meant are all carbohydrates comprising six carbon atoms.
• “Monosaccharides and / or disaccharides and / or trisaccharides and / or tetrasaccharides” is meant any saccharide comprising a saccharide or two, three or four saccharides linked together.
• the cotton fiber extract comprises a hydrolyzate of cotton fibers hydrolyzed enzymatically according to the process described hereinafter.
• the monosaccharides, disaccharides, trisaccharides and / or tetrasaccharides are glucose, cellobiose, cellotriose, cellotetraose and cellulose oligomers.
• the cotton fiber extract further comprises at least one additional saccharide.
• the saccharide (s) are preferably of natural origin, and preferably glucose and / or fructose and / or trehalose and / or sucrose and / or inositol.
• the cotton fiber extract advantageously further comprises the following additional saccharides: glucose, fructose, trehalose, sucrose and inositol.
• the cotton fiber extract of the invention comprises about 2% cotton fiber hydrolyzate, 0.8% glucose, 0.8% fructose, 1.1% trehalose, 7% sucrose and 0.5% inositol.
• the present invention relates to a cosmetic composition
• a cosmetic composition comprising as active ingredient, in a physiologically suitable medium, an extract of cotton fibers as described below.
• physiologically suitable medium means a medium compatible with the skin, hair or hair.
• the cosmetic composition according to the invention comprises, as active principle, a natural product of plant origin, the cotton fiber extract, which has been hydrolysed in an enzymatic manner according to the process described hereinafter.
• the inventors have demonstrated, surprisingly, that the cosmetic compositions comprising, as active principle, a cotton fiber extract according to the invention, make it possible in particular to protect the cells against osmotic shocks, to obtain protection against damage to the DNA of the cells, in particular that the cosmetic compositions make it possible to protect the DNA of the cells when they are subjected to stresses such as, for example, the deprivation of nutrients.
• the cosmetic compositions have a protective effect on keratinocytes and on DNA and make it possible in particular to moisturize the skin.
• These protective properties of the cosmetic compositions according to the invention can be used, for example, to protect the skin and / or the hair against external aggressions caused, in particular, by the action of solar radiation or by other physical or chemical agents. or biological. These protective properties can also be used to combat the aging of the skin.
• active ingredient according to the invention can be used alone or in combination with other active ingredients.
• the cosmetic composition according to the invention it is possible to add to the cosmetic composition according to the invention other active ingredients intended in particular for the prevention and / or treatment of cutaneous manifestations of aging and / or the protection of the skin and / or the hair against external aggression.
• the cosmetic compositions that can be used according to the invention may contain, in addition, at least one other active ingredient intended to reinforce the action of the cotton fiber extract according to the invention, in the field of prevention and control. improvement of the cutaneous signs of aging or making it possible to widen the range of properties of the cosmetic composition according to the invention.
• the following classes of ingredients may be mentioned in the cosmetic field: regenerating, anti-aging, anti-wrinkle, soothing, antioxidant, healing, relipidant, nutritive, anti-free radical, anti-glycation, moisturizing agents, antibacterial, antifungal, keratolytic, myorelaxant, desquamating, firming agents, agents stimulating the synthesis of dermal macromolecules or energy metabolism or microcirculation or nail growth or hair growth, agents modulating the differentiation or the pigmentation of the epidermis , metalloproteinase inhibiting agents or sunscreens.
• the cosmetic composition according to the invention will comprise, in addition to the cotton fiber extract according to the invention,
• At least one hydrating compound such as an activator compound for aquaporins and / or,
• At least one activator compound for sirtuins and / or,
• At least one circadian rhythm modulating compound and / or,
• At least one HSP protein modulating compound and / or,
• barrier-improving compound such as an activator compound of transglutaminase or HMG-CoA reductase and / or,
• At least one compound that protects the mitochondria at least one compound that protects the mitochondria.
• Said above compounds may be of natural origin, such as peptide extracts of plants, or of synthetic origin, such as peptides.
• the other active agents or active principles associated with the active agent or active principle according to the invention in the composition may have very diverse chemical structures.
• the cosmetic composition according to the invention may further comprise at least one compound that improves the health of the hair.
• vitamins may in particular be made of vitamins, other plant peptide extracts, minoxidil, nicotinic acid esters, trace elements, anti-inflammatory agents, retinoic acid or its derivatives, retinol, inhibitors 5 ⁇ -reductase or peptide compounds from chemical synthesis.
• vitamins include vitamins A, E, B5, B6, C, H, or PP, as an example of trace elements include zinc, copper, magnesium, or silicon.
• the cotton fiber extract is present in the cosmetic composition at a concentration of between 0.01% and 20% by weight. weight relative to the total weight of the composition, and preferably at a concentration of between 0.1% and 10% by weight relative to the total weight of the composition.
• the cosmetic composition according to the invention also comprises an amount of between 0.01% and 5% by weight relative to the total weight of the composition of at least one additional saccharide such as glucose and / or fructose and / or trehalose and / or sucrose and / or inositol.
• additional saccharide such as glucose and / or fructose and / or trehalose and / or sucrose and / or inositol.
• the active ingredient in addition to the monosaccharides and / or disaccharides and / or trisaccharides and / or tetrasaccharides, comprises saccharides which are added to the composition initially obtained by enzymatic hydrolysis of cotton fibers.
• These saccharides are preferably glucose and / or fructose and / or trehalose and / or sucrose and / or inositol.
• the cosmetic composition according to the invention comprises approximately 2% cotton fiber extract, 0.8% glucose, 0.8% fructose, 1.1% of trehalose, 1.7% of sucrose and 0.5% of inositol.
• compositions according to the present invention are intended for the care of keratinous substrates.
• keratinous substrate is intended to mean all the substrates which are composed largely of keratin. These are substrates such as skin, hair, eyelashes, eyebrows or even nails and integuments in general. Keratin substrates are all actions intended to maintain or restore a proper functioning of this substrate, or any means that serves to preserve or improve its appearance.
• the care includes hydration, soothing, protection against all types of aggression, including sun protection, the fight and the prevention of the manifestations of aging, including skin manifestations of aging.
• “Cutaneous manifestations of aging” means any changes in the external appearance of the skin due to aging, such as, for example, wrinkles and fine lines, wilted skin, soft skin, thinned skin, lack of elasticity and / or skin tone, dull and lackluster skin, but also any internal changes in the skin that do not systematically result in a modified external appearance, such as, for example, any internal skin damage resulting from exposure to radiation ultraviolet.
• the use of detergent lotions (such as shampoos and other soaps) essentially based on conventional surfactants, especially of the anionic, non-anionic and / or or amphoteric, but more particularly of anionic type, is common.
• compositions are applied to wet keratinous substrates and the foam generated by the massage makes it possible, after rinsing with water, to eliminate the various soils initially present.
• These compositions have, of course, a good washing power, but the cosmetic properties attached to them remain rather weak considering the relatively aggressive nature of such a cleaning treatment. Indeed, this treatment may, in the long run, cause more or less marked damage to the hair fiber and / or the skin, in particular linked to a progressive elimination of the proteins contained in or on the surface of the latter.
• one solution is to introduce complementary cosmetic agents intended primarily to repair or limit the harmful or undesirable effects induced by the various treatments or attacks that the hair fibers undergo and the skin, ie to protect.
• These cosmetic agents may be, for example, a cotton fiber extract according to the invention or a cosmetic composition according to the invention.
• the cosmetic compositions according to the invention are intended to improve the appearance and the quality of the keratin substrates of the individual who makes use of them.
• compositions according to the present invention are in a dosage form adapted for topical administration to the skin and adapted for administration to the hair. It covers all cosmetic forms. These compositions must therefore contain a physiologically acceptable medium, that is to say, compatible with the skin, hair or hair. These compositions may especially be in the form of an aqueous solution, hydro-alcoholic or oily; an oil-in-water, water-in-oil emulsion or multiple emulsions; they can also be in the form of creams, suspensions or powders, suitable for application to the skin, mucous membranes, lips and / or hair.
• compositions may be more or less fluid and have the appearance of a cream, a lotion, a milk, a serum, an ointment, a shampoo, a gel, a paste or foam. They can also be in solid form, as a stick or be applied to the skin in aerosol form. They can be used as a care product and / or as a make-up product for the skin.
• compositions additionally comprise any additive usually used in the intended field of application and the adjuvants necessary for their formulation, such as solvents, thickeners, diluents, antioxidants, dyes, sunscreens, self-tanning agents, pigments, fillers, preservatives, perfumes, odor absorbers, cosmetic active agents, essential oils, vitamins, essential fatty acids, surfactants, film-forming polymers, etc.
• solvents such as solvents, thickeners, diluents, antioxidants, dyes, sunscreens, self-tanning agents, pigments, fillers, preservatives, perfumes, odor absorbers, cosmetic active agents, essential oils, vitamins, essential fatty acids, surfactants, film-forming polymers, etc.
• adjuvants may, for example, be present at a concentration of 0.01 to 20% of the total weight of the composition.
• the fatty phase can represent from 5 to 80% by weight and preferably from 5 to 50% by weight relative to the total weight of the composition.
• the emulsifiers and co-emulsifiers used in the composition will be chosen from those conventionally used in the field under consideration. For example, they can be used in a proportion ranging from 0.3 to 30% by weight, relative to the total weight of the composition.
• those skilled in the art will take care to choose any additional compounds, active or non-active, and / or their amounts, so that the advantageous properties of the mixture are not or not substantially, altered by the addition envisaged .
• compositions according to the invention find an application in particular as a cosmetic composition for the skin, the mucous membranes and / or semi-mucous membranes, but also as a cosmetic composition for the hair and / or the hair. It is also possible to envisage an application in the field of make-up compositions of the skin of the face and of the body, such as lipsticks, foundations, tinted creams, concealer sticks, anti-solar compositions or artificial tanning.
• compositions which are the subject of the invention, find their applications in a large number of cosmetic applications, in particular for the treatment, protection, care, removal of make-up and / or cleansing of the skin, lips and / or hair. and / or for makeup of the skin, lips, eyelashes and / or body.
• the cosmetic compositions according to the invention may also consist of solid preparations also comprising soaps or cleaning bars.
• the cosmetic compositions may also be packaged in the form of an aerosol composition also comprising a propellant under pressure.
• the compositions may also be for oral use, for example a paste of toothpaste.
• the cosmetic composition according to the invention may be intended to form a protective filter at the keratin substrates.
• the third subject of the invention is a cosmetic treatment method for protecting keratinous substrates, and more particularly for protecting the skin and the hair, against all types of external aggressions consisting in applying keratinous substrates to the surface, and more particularly on the skin and / or the hair, an effective amount of a cosmetic composition according to the invention.
• the invention also relates to a cosmetic treatment method for reinforcing the cutaneous barrier of the skin and / or for reinforcing the protection of the hair and / or for increasing the synthesis of keratin and / or for nourishing the keratinous substrates, and more particularly the skin and the hair, consisting in applying to the surface of the skin and / or the hair, an effective amount of a cosmetic composition according to the invention.
• the fourth subject of the invention is a process for preparing a cotton fiber extract comprising the following steps:
• a-reduced powdered cotton fibers are stirred in osmosis water in the presence of cellulases,
• the hydrolysis is carried out for a period of between 15 and 24 hours at a temperature of about 50 ° C., to obtain a hydrolyzate of the cotton fibers
• step b of the hydrolysis is carried out for a period of about 18 hours.
• Cotton fibers (genus Gossypium, preferentially Gossypium hirsutum, Gossypium herbaceum, and even more preferably Gossypium hirsutum) are reduced to powder.
• crude fibers pretreated with 14% sodium hydroxide solution can be used for at least 12 hours.
• the mixture is filtered, concentrated or diluted to obtain a concentration of between 20 and 50 g / kg of saccharides of the monosaccharide and / or disaccharide and / or trisaccharide and / or tetrasaccharide type and more particularly glucose and / or cellobiose and / or cellotrioses and / or cellotetraose and / or cellulose oligomers.
• glycerol About 30% by weight of glycerol is added.
• the mixture is filtered by filtration on porosity plates decreasing to 0.2 ⁇ and pasteurized at low temperature.
• one or more pure sugars (saccharides) of plant origin may be added, before the filtration step in the proportions indicated below:
• the final total sugar concentration of the cotton fiber extract, with or without added sugar is between 55 and 75 g / kg.
• the fifth subject of the invention is the use of a cotton fiber extract obtained according to the method of preparation described above for the preparation of a cosmetic composition according to the invention.
• Example 1 Process for preparing the cotton fiber extract
• the hydrolysis is carried out by maintaining this mixture for 18 hours at 50 ° C.
• the enzymes are then deactivated by heating at 80 ° C. for 2 hours and the mixture is filtered (standard filtration step, preferably bell filter).
• the extract contains only sugars, at a concentration of 40 g / kg of total sugars.
• the determination of total sugars is carried out by the Sucre-phenol technique.
• This extract is then filtered and diluted to a final concentration of 20 g / kg of total sugars.
• the analysis by thin layer chromatography shows that the extract contains monosaccharides, disaccharides, trisaccharides and tetrasaccharides and more particularly glucose, cellobiose, cellotrioses, cellotetraose and cellulose oligomers.
• the extract is diluted in a mixture of osmosis water and 30% of glycerol in order to obtain a concentration of total sugars of 20 g / kg.
• the mixture is filtered by filtration on plates of decreasing porosity up to 0.2 ⁇ . and pasteurized at low temperature.
• the final concentration of the extract is 60 g / kg of total sugars.
• Example 2 Demonstration of the effect of the cotton fiber extract on the synthesis of keratin.
• the experiments were performed on sections of human skin cultured for 24 hours.
• the expression of keratin was studied on these sections of skin, thanks to the method of immunofluorescence staining.
• the cotton fiber extract prepared in Example 1 was diluted in a "phosphate buffer saline" buffer (PBS) to obtain a final concentration of 0.5% and then the dilution was applied to skin cuts, for two applications.
• PBS phosphate buffer saline buffer
• Cells are pretreated for 2 hours in the presence of 0.5% extract.
• the control condition carried out consists of cells not treated with the extract according to the invention. Then, the culture medium, containing or not containing the extract, is replaced by PBS for a period of 3 hours.
• MTT tests were performed using the MTT test.
• the MTT agent is used to evaluate the cytotoxicity of a product against a cell medium by measuring cell viability.
• the keratinocytes are incubated in a solution containing 0.1 mg / ml of MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide).
• MTT 3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide.
• This compound is absorbed by living cells and then metabolized by the mitochondrial enzymes (succinate dehydrogenase) into a blue violet compound, formazan, which is in the form of violet crystals insoluble in aqueous medium.
• the formazan crystals are solubilized in DMSO, they give an optical density (OD) proportional to the number of living cells present in the sample.
• Optical density measurements were then performed for each sample studied (D.O. reading at 540 nm). The O.D. is then directly proportional to the enzymatic activity as well as to the number of living cells.
• Example 4 Demonstration of the protective effect of the cotton fiber extract against osmotic shock.
• hypertonic medium i.e., containing DMEM and 500 mM NaCl
• the result of this test demonstrates that the viability of the cells is halved when they are subjected to osmotic shock.
• the results demonstrate that the pretreatment with the cotton fiber extract increases the viability of keratinocytes by 32% compared to the control cells, that is to say, not treated with the extract but having undergone the osmotic shock. .
• Example 5 Demonstration of the effect of the cotton fiber extract on the protection of DNA.
• Example 3 The same test as that of Example 3 was carried out: the cells were subjected to stress caused by a starvation.
• the "Single Cell Gel Electrophoresis” test is a fast agarose microgel electrophoresis technique that allows the visualization of single and double-strand DNA breaks on individual cells.
• the cells After treatment, the cells are trapped in an agarose gel and lysed in a high salinity buffer containing detergents.
• the DNA is then denatured by alkaline bath followed by a short electrophoresis, and is then evidenced by propidium iodide.
• the DNA of an altered cell stretches toward the anode in proportion to the number of breaks, taking more or less the shape of a comet.
• the highly degraded DNA is found in the "tail" of the comet. A intact cell remains round, the remaining DNA compacted at the level of the "head" of the comet.
• DNA damage is assessed using software that determines the percentage of DNA degradation.
• the results show that nutrient-starved cells are stressed and their DNA degraded.
• the results demonstrate that DNA protection of cells treated with cotton fiber extract is increased by 45% over control cells (i.e. cells not treated with extract but having suffered stress). The cotton fiber extract therefore plays an important role in the protection of DNA.
• Example 6 Hydration test on volunteers (Clinical test)
• the cotton fiber extract according to Example 1 was administered topically at 1.5% in a standard cosmetic formulation to obtain a cosmetic composition. Only one application was performed by an expert.
• the skin was observed by a non-invasive technique, using a Vivascope TM.
• the quantitatively evaluated criteria were the thickness of the stratum corneum and the thickness of the stratum granulosum. Result: A significant decrease in the thickness of the stratum corneum was observed after 3 hours of application of the cosmetic composition comprising the cotton fiber extract.
• the cotton fiber extract according to Example 1 was administered topically at 1.5% in a standard cosmetic formulation to obtain a cosmetic composition. Only one application was performed by an expert.
• the evaluation method used is the CM825 Corneasometer.

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• 2012
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