WO2013170229A1 - Détection d'antigènes tels que des protéines de détection de quorum bactérien - Google Patents

Détection d'antigènes tels que des protéines de détection de quorum bactérien Download PDF

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Publication number
WO2013170229A1
WO2013170229A1 PCT/US2013/040674 US2013040674W WO2013170229A1 WO 2013170229 A1 WO2013170229 A1 WO 2013170229A1 US 2013040674 W US2013040674 W US 2013040674W WO 2013170229 A1 WO2013170229 A1 WO 2013170229A1
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WO
WIPO (PCT)
Prior art keywords
nanoparticles
nanoparticle
antigen
composition
target antigen
Prior art date
Application number
PCT/US2013/040674
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English (en)
Inventor
Charleson S. Bell
Todd D. Giorgio
Original Assignee
Vanderbilt University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vanderbilt University filed Critical Vanderbilt University
Publication of WO2013170229A1 publication Critical patent/WO2013170229A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Nanotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La présente invention concerne des compositions et des procédés pour détecter la présence ou la concentration d'un antigène cible dans une solution. Les compositions comprennent une pluralité de nanoparticules, chacune comprenant un noyau comprenant de l'oxyde de fer, et une enveloppe comprenant au moins un métal actif plasmonique, une surface extérieure de chaque nanoparticule étant fonctionnalisée avec une pluralité d'anticorps polyclonaux, chacun étant indépendamment configuré pour se lier à au moins une partie de l'antigène cible. Les procédés comprennent la préparation d'une solution comprenant la composition et l'antigène cible, au moins une partie de la pluralité de nanoparticules se liant à l'antigène cible pour former un agrégat nanoparticule-antigène. Les nanoparticules qui forment l'agrégat nanoparticule-antigène absorbent un rayonnement électromagnétique différemment des nanoparticules qui ne forment pas l'agrégat nanoparticule-antigène.
PCT/US2013/040674 2012-05-11 2013-05-11 Détection d'antigènes tels que des protéines de détection de quorum bactérien WO2013170229A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261688337P 2012-05-11 2012-05-11
US61/688,337 2012-05-11

Publications (1)

Publication Number Publication Date
WO2013170229A1 true WO2013170229A1 (fr) 2013-11-14

Family

ID=49551324

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/040674 WO2013170229A1 (fr) 2012-05-11 2013-05-11 Détection d'antigènes tels que des protéines de détection de quorum bactérien

Country Status (1)

Country Link
WO (1) WO2013170229A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110726841A (zh) * 2019-10-21 2020-01-24 华中农业大学 一种基于酶促点击反应信号放大磁弛豫时间免疫传感器检测兽药残留的方法

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4698302A (en) * 1983-05-12 1987-10-06 Advanced Magnetics, Inc. Enzymatic reactions using magnetic particles
US20020177143A1 (en) * 2001-05-25 2002-11-28 Mirkin Chad A. Non-alloying core shell nanoparticles
WO2006102307A2 (fr) * 2005-03-21 2006-09-28 University Of Louisville Research Foundation, Inc. Nanoparticules specifiques d'une cible destinees a ameliorer le contraste optique et a induire l'hyperthermie specifique de la cible
WO2008124525A1 (fr) * 2007-04-03 2008-10-16 Vanderbilt University Nanoparticules avec des éléments de reconnaissance moléculaires
WO2008140624A2 (fr) * 2006-12-22 2008-11-20 The Board Of Regents Of The University Of Texas System Méthodes et compositions associées à des nanoparticules hybrides
US20090203154A1 (en) * 2006-06-06 2009-08-13 Vivacta Ltd. Method for Sensing a Chemical
US7700193B2 (en) * 2005-04-08 2010-04-20 Industrial Technology Research Institute Core-shell structure with magnetic, thermal, and optical characteristics and manufacturing method thereof
US7801590B2 (en) * 2005-05-27 2010-09-21 Board Of Regents, The University Of Texas System Optical coherence tomographic detection of cells and killing of the same

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4698302A (en) * 1983-05-12 1987-10-06 Advanced Magnetics, Inc. Enzymatic reactions using magnetic particles
US20020177143A1 (en) * 2001-05-25 2002-11-28 Mirkin Chad A. Non-alloying core shell nanoparticles
WO2006102307A2 (fr) * 2005-03-21 2006-09-28 University Of Louisville Research Foundation, Inc. Nanoparticules specifiques d'une cible destinees a ameliorer le contraste optique et a induire l'hyperthermie specifique de la cible
US7700193B2 (en) * 2005-04-08 2010-04-20 Industrial Technology Research Institute Core-shell structure with magnetic, thermal, and optical characteristics and manufacturing method thereof
US7801590B2 (en) * 2005-05-27 2010-09-21 Board Of Regents, The University Of Texas System Optical coherence tomographic detection of cells and killing of the same
US20090203154A1 (en) * 2006-06-06 2009-08-13 Vivacta Ltd. Method for Sensing a Chemical
WO2008140624A2 (fr) * 2006-12-22 2008-11-20 The Board Of Regents Of The University Of Texas System Méthodes et compositions associées à des nanoparticules hybrides
WO2008124525A1 (fr) * 2007-04-03 2008-10-16 Vanderbilt University Nanoparticules avec des éléments de reconnaissance moléculaires

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110726841A (zh) * 2019-10-21 2020-01-24 华中农业大学 一种基于酶促点击反应信号放大磁弛豫时间免疫传感器检测兽药残留的方法

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