WO2013149951A1 - Module séparateur de couleurs imageur et procédé permettant d'obtenir une image d'un champ d'objet dans un premier plan d'image et dans un second plan d'image - Google Patents

Module séparateur de couleurs imageur et procédé permettant d'obtenir une image d'un champ d'objet dans un premier plan d'image et dans un second plan d'image Download PDF

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Publication number
WO2013149951A1
WO2013149951A1 PCT/EP2013/056759 EP2013056759W WO2013149951A1 WO 2013149951 A1 WO2013149951 A1 WO 2013149951A1 EP 2013056759 W EP2013056759 W EP 2013056759W WO 2013149951 A1 WO2013149951 A1 WO 2013149951A1
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WO
WIPO (PCT)
Prior art keywords
beam path
color
image plane
imaging
lens
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PCT/EP2013/056759
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German (de)
English (en)
Inventor
Sebastian Borck
Lars-Christian Wittig
Original Assignee
Carl Zeiss Microscopy Gmbh
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Application filed by Carl Zeiss Microscopy Gmbh filed Critical Carl Zeiss Microscopy Gmbh
Publication of WO2013149951A1 publication Critical patent/WO2013149951A1/fr

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/1006—Beam splitting or combining systems for splitting or combining different wavelengths
    • G02B27/1013—Beam splitting or combining systems for splitting or combining different wavelengths for colour or multispectral image sensors, e.g. splitting an image into monochromatic image components on respective sensors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/1006—Beam splitting or combining systems for splitting or combining different wavelengths
    • G02B27/102—Beam splitting or combining systems for splitting or combining different wavelengths for generating a colour image from monochromatic image signal sources
    • G02B27/104—Beam splitting or combining systems for splitting or combining different wavelengths for generating a colour image from monochromatic image signal sources for use with scanning systems

Definitions

  • the present invention relates to an imaging color splitter module and to a method for imaging an object field into a first and a second image plane.
  • imaging color divider modules are e.g. used in fluorescence microscopy of biological samples to stimulate multi-stained samples simultaneously and simultaneously observe or record two fluorescence images.
  • a color splitter module preferably monochrome cameras are used, so that a color division is performed by means of a color splitter module.
  • a color splitter module usually includes imaging optics as well as a 45 ° beam splitter and two emission filters, the beam splitter being e.g. is arranged in a parallelized or a convergent beam path. As a result, the light strikes the beam splitter with a respectively different angular spectrum for different points of the object field to be imaged.
  • both the beam splitter and the emission filters must be designed as dielectric elements, which leads to high costs in the color splitter module due to the high cost of such dielectric elements. Furthermore, the sensitivity is reduced by the finite efficiency of the dielectric elements and there are many interfering interfaces (of the three dielectric elements).
  • the properties of such a dielectric beam splitter are angle dependent and have e.g. a [1 -cos (a) j characteristic of the spectral properties as a function of the light incidence angle, which consequently leads to a spectrally inhomogeneous division over the field. Furthermore, the location of the transition between the reflection and transmission bands is generally different for the p and s polarizations. Since the light to be detected is usually unpolarized in fluorescence applications, a spectral spread of the transition region results disadvantageously.
  • an object of the invention to provide an imaging color splitter module with improved properties. Furthermore, an improved Method for the spectrally selective imaging of an object field in a first and a second image plane are provided.
  • an imaging color splitter module with a first and a second lens having imaging optics for imaging an object field in a first and a second image plane and a color splitter, the one extending from the object field to the color splitter common beam path in a first beam path to extends to the first image plane, and in a second beam path, which extends to the second image plane, divides, wherein the common beam path and the first beam path each extend out of axis through the first lens and the second beam path out of axis through the second lens.
  • the corresponding beam path is not symmetrical to the optical axis of the imaging optics or that the corresponding beam path is adjacent to the optical axis or from this, so that the optical axis ( in the region of the corresponding lens) is not in the corresponding beam path.
  • the beam path is understood here in particular to mean that this is the area in which the light propagates in the desired intended imaging.
  • the imaging color splitter module according to the invention is also referred to below as an imaging spectral multi-channel system.
  • the color splitter may be a special dielectric filter which, by virtue of its particular arrangement in the beam path and design, combines the functionalities of a color splitter and an emission filter into a single element. This is referred to below as a combination filter.
  • the color splitter can thus advantageously combine the properties of a color splitter and an emission filter (preferably for the second beam path).
  • a spectrally selective imaging of the object field in the first and second image plane can be performed with the imaging optics, wherein the spectral splitting is performed by means of the color splitter or the combination filter, so that no additional beam splitter, as in conventional color splitter modules, is necessary.
  • the color splitter module according to the invention can therefore also be referred to as a beam splitterless color splitter module or beam splitterless multi-channel system.
  • an imaging color splitter module which has a first curved mirror surface and a second curved mirror surface imaging optics for imaging an object field in a first and a second image plane and a color splitter, extending from the object field to the color splitter common beam path in a first beam path, which extends to the first image plane and in a second beam path, which extends to the second image plane, divides, wherein the common beam path is folded at the first curved mirror surface and the first and the second beam path respectively at the second curved mirror surface folded.
  • the imaging color splitter module with the aid of reflective optics, it is advantageously achieved that the imaging by means of the imaging optics can be used free from color aberration and therefore very broadband.
  • the overall length of the structure is lower compared to the variant with lenses. Due to the inherent folding of the beam path, the overall length of the same focal length is only half as large.
  • the location of the convolution of the first beam path on the second curved mirror surface may be spaced from the location of the convolution of the second beam path on the second curved mirror surface.
  • the two curved mirror surfaces may be part of a contiguous mirror surface.
  • the coherent mirror surface may be formed as a spherically curved mirror surface.
  • the two mirror surfaces can be separated from each other.
  • the two mirror surfaces may have different curvatures.
  • the color splitter can be designed to be reflective for the first and second beam paths.
  • the imaging optics can be designed in particular as a 1: 1 imaging optics.
  • the imaging optics may have a pupil and the color splitter is preferably arranged in the pupil.
  • the color splitter is arranged in the pupil between both lenses.
  • the color splitter can effect a reflection for the first beam path and a transmission for the second beam path.
  • the imaging optics may preferably be designed as telecentric 4f imaging optics. Between the first lens and the first image plane, a further color filter is preferably arranged. Furthermore, a further color filter can be arranged between the second lens and the second image plane.
  • the color filter or combination filter and / or the further color filter or the further combination filter can be designed, in particular, as an interference filter and / or as an emission filter. Furthermore, in the case of the imaging color splitter module, the color splitter can be provided interchangeably.
  • the imaging color splitter module can be designed as a camera adapter, so that the first and second image can be recorded simultaneously. This can be done by one or two cameras.
  • a second imaging color splitter module which has a second imaging optics having a third lens for imaging a further object field into a third and fourth image plane and a further color splitter which converts a further common beam path extending from the further object field into a third beam path. which extends to the third image plane, and in a fourth beam path, which extends to the fourth image plane, splits, wherein the further common beam path and the third beam path through the third lens and wherein either a) the further object field with the first or second image plane coincides or is imaged on it or b) the object field coincides with the third or fourth image plane or is imaged on it.
  • An intermediate imaging optics can be provided between the two color divider modules.
  • the second color divider module can be formed and developed in the same way as the color divider module according to the invention. Furthermore, it is possible that in the second color divider module the further color divider is followed by a reflective element which causes a convolution of the fourth beam path, so that the fourth beam path passes through the further color divider and the third lens.
  • the second imaging optic preferably includes only the third lens.
  • the further color splitter and the reflective element are preferably arranged so that after the beam path convolution caused by both elements, the third and fourth beam paths do not coincide, but include, for example, a predetermined angle.
  • At least one third color splitter can be arranged between the further color splitter and the reflective element, which causes a further beam path convolution for a fifth beam path (or further beam paths) for a predetermined wave range, which coincides neither with the third nor with the fourth beam path ,
  • the object field can be spectrally selectively imaged in five or more image planes by means of the color divider module according to the invention.
  • the third and fourth image planes may coincide.
  • the color splitter module according to the invention can be used in a microscope.
  • a microscope in particular a light-scanning microscope or a laser scanning microscope, is thus also provided with a color splitter module according to the invention.
  • the object is further achieved by a method for the spectrally selective imaging of an object field in a first and a second image plane having a first and a second lens having imaging optics and a color splitter in which by means of the color splitter from the object field to the color splitter extending common beam path in a first beam path, which extends to the first image plane, and in a second beam path, which extends to the second image plane, is divided so that both the common beam path and the first beam path are each out of axis through the first lens and the second beam path off-axis passes through the second lens.
  • the object is also achieved by a method for imaging an object field in a first and in a second image plane with a first curved mirror surface and a second curved mirror surface having imaging optics and a color splitter, in which by means of the color divider extending from the object field to the color divider common optical path in a first beam path, which extends to the first image plane, and in a second beam path, which extends to the second image plane, is divided so that the common beam path is folded at the first curved mirror surface and the first and second beam path respectively the second curved mirror surface is folded.
  • FIG. 1 shows a schematic view of a first embodiment of the invention
  • Color divider module 1
  • Fig. 2 is a schematic plan view of the lens 2 of Fig. 1; 3 is a schematic detail view for explaining the image of the object field 5 in the first image plane 6;
  • Fig. 4 shows schematically the combination of several color divider modules
  • Fig. 5 is a schematic view of an embodiment of another color divider module
  • Fig. 6 is another view for explaining the embodiment of FIG. 5;
  • Fig. 7 is a plan view of the lens 2 'of the embodiment of Figs. 5 and 6;
  • FIG. 8 is a view for explaining the image of the object field in the first image plane in the embodiment according to FIGS. 5 to 7;
  • FIG. Fig. 9 is a view for explaining a modification of the embodiment according to
  • FIG. 13 ' a schematic representation of a microscope with a color splitter module according to the invention
  • 16 shows a schematic view of an embodiment of the color divider module 1 according to the invention with an imaging mirror
  • FIG. 17 shows a further view of the embodiment according to FIG. 16;
  • FIG. 18 is a schematic plan view of the mirror 50 of FIGS. 16 and 17, and FIG.
  • FIG. 19 is a view according to FIG. 17 of a further modification of the invention.
  • Color divider module 1 1.
  • the imaging color splitter module 1 comprises a first and a second lens 2, 3, which form an imaging optical unit 4.
  • the imaging optics 4 is designed such that it images an object field 5, which may be, for example, an intermediate image of a microscopic image, into a first image plane 6 and a second image plane 7.
  • the first and second lenses 2, 3 each have the same focal length f and lie on a common optical axis 8.
  • the distance between the two lenses 2, 3 is 2f.
  • the distance of the object field or the plane in which the object field 5 lies to the first lens 2 is f.
  • the distance between the first image plane 6 and the first lens 2 is again f and also the distance between the second lens 3 and the second image plane 7 is f.
  • the imaging optics 4 is thus designed as a 1: 1 imaging optics and can also be referred to as telecentric 4f imaging optics.
  • the imaging optics 4 has a pupil 9 between both lenses 2, 3, in which a first color filter 10 is arranged, which transmits light of a predetermined wavelength range and reflects light outside the predetermined wavelength range. Furthermore, in the pupil 9 still a diaphragm 1 1 is provided.
  • the light of the object field 5 runs off-axis through the first lens 2 and impinges on the first color filter 10.
  • This color filter 10 can in particular be a special dielectric filter which, due to its special arrangement in the beam path and design, the functionalities a color splitter and an emission filter in a single element. This is referred to below as a combination filter.
  • the light reflected by the first color filter 10 with a wavelength outside the predetermined wavelength range thus again passes through the first lens 2, strikes a deflection mirror 12, passes through a second color filter 13 and then strikes the first image plane 6
  • Color filter 13 is designed to transmit only light of a first wavelength range different from the predetermined wavelength range (hereinafter also referred to as second wavelength range).
  • the light transmitted by the first color filter 10 hits the second lens 3 off-axis and is imaged by the latter into the second image plane 7.
  • the color splitter module 1 thus has a common beam path 14, which runs from the object field 5 through the first lens 2 to the first color filter 10.
  • this common beam path 14 both light of the first wavelength range and light of the second wavelength range is included.
  • the first color filter 10 is now used in addition to its property as a color filter as a divider, the common beam path 14 in a first beam path 15, the first color filter 10 via the first lens 2, the deflection mirror 12, the second color filter 13 to the first image plane. 6 runs, and in a second beam path 16 which extends from the first color filter 10 via the second lens 3 to the second image plane 7 divides.
  • the object field 5 is imaged both in the first and in the second image plane 6, 7, wherein in the image in the first image plane 6 is a folded beam path and the first lens 2 is traversed twice and in the image in the second image plane. 7 no folded beam path is present and the first lens 2 and the second lens 3 is traversed exactly once.
  • the optical structure of the color divider module 1 can therefore be referred to as laterally asymmetric, telecentric structure.
  • the object field 5 can be imaged into the second image plane 7 as first image 17 only with light of the first wavelength range in the first image plane 6 and as second image 18 only with light of the second wavelength range.
  • FIG. 2 shows a plan view of the first lens 2, wherein schematically the passage areas for the object field 5 and the first image 17 and thus for the common beam path 14 and the first beam path 15 are shown.
  • the beam paths 14 and 15 go off-axis through the first lens.
  • the second beam path 16 goes out of axis through the second lens 3, as shown in Fig. 1.
  • Fig. 3 only the common beam path 14 and the first beam path 15 is shown in order to illustrate the imaging property of the imaging optics 4.
  • the color splitter module 1 according to the invention in addition to the two color filters, no additional beam splitter is necessary in comparison to conventional color splitter modules, since the first color filter 10 simultaneously serves as a beam splitter in the color splitter module 1 according to the invention.
  • the number of components can be reduced, which allows a more cost-effective production of the color divider module 1 according to the invention.
  • the color splitter module 1 according to the invention also has fewer surfaces, so that fewer losses occur. Since the first color filter 10 is arranged in the parallel beam path between the two lenses 2 and 3, there is no increased susceptibility to astigmatism.
  • the color filters 10, 13 can have steeper spectral edges due to a lower polarization splitting of the transition regions. Furthermore, there is a smaller angle dependence of the spectral division properties, so that a spectrally constant division over the image field is possible.
  • the second image 18 of the color divider module 1 can serve as an object field of a further color divider module 1 'according to FIGS. 1 to 3.
  • the order of the color divider modules 1, 1 ' can of course also be reversed so that a color divider module 1 is arranged downstream of a color divider module 1'.
  • a possible embodiment of a further color divider module 1 ' is shown, which is similar to the embodiment according to FIGS. 1 to 3.
  • the essential difference is that the imaging optics 4 'only a single lens 2', which is traversed by both the common beam path 14 'and the first and second beam paths 15' and 16 '. Due to the similar design, similar elements are denoted by reference numerals, which are marked to distinguish only with the sign '.
  • the common beam path 14 passes through the first lens 2' off-axis and strikes the first color filter 10 ', which now with the optical axis 8' an angle of not equal to 90 °, wherein behind the first color filter 10 a oppositely tilted mirror 19 'is arranged (Fig. 6). Furthermore, a second deflecting mirror 20 'is provided for the second beam path 16'.
  • the second beam path 16 'in the lens 2' is offset from the common beam path 14 'in both the x and y directions.
  • the offset in the x direction is the same as in the first beam path 15 '.
  • the offset in the y-direction is equal to the absolute value, but in the opposite direction compared to the first beam path 15 ', as shown in Fig. 7 can be seen.
  • the beam path for the image in the first image plane 6 'in the same manner as in Fig. 3 is shown.
  • the embodiment according to FIGS. 6 to 8 can be extended to more than two channels.
  • FIGS. 5 to 8 A modification of the embodiment according to FIGS. 5 to 8 will be explained below in conjunction with FIG. 9, the illustration in FIG. 9 corresponding to the representation in FIG. 6.
  • These Modification differs from the embodiment of FIG. 5 to 8 essentially only in that the first color filter 1 0 'as a backside mirrored color filter 10' with a defined keilwinkligen substrate and thus monolithic. Otherwise, the structure is the same as in the embodiment according to FIGS. 5 to 8, so that reference may be made to the corresponding description of FIGS. 5 to 8.
  • the color divider module according to the invention can be designed, for example, as a camera adapter.
  • the first and second image plane 6, 7 cameras may be provided to accommodate the spectrally different images 17, 18.
  • the color divider modules 1 'according to FIGS. 5 to 8 and FIG. 9 can also be designed such that the two spectrally different images 17', 18 'are recorded with a single camera chip.
  • the two mirrors 12 'and 20' are omitted, so that the first and second image plane 6 ', 7' coincide and lie in the xy plane, whereby in the xy plane the first and second image 17 ', 18' in the y-direction are next to each other.
  • a correspondingly arranged camera chip 38 ' is shown schematically in FIGS. 5 and 8 and the corresponding beam path is shown in dashed lines.
  • the color filters 10, 13 and 10 ', 13' are in particular interference filters or emission filters. They can each be designed as a long, band or short-pass filter.
  • the color filters 10, 13 or 10 ', 13' can be provided interchangeably and / or with variable filter properties.
  • a rotatable filter wheel 25 (Fig. 10) with several different filters 26, 27, 28, 29 may be provided, wherein by rotation of the filter wheel always one of the filter in the working position as the first color filter 10 or 10 'or second color filter 13 or 13th 'can be brought and the arrow indicates P1 in the direction of the incident light.
  • two filter wheels 30, 31 may be arranged one behind the other, as indicated in Fig. 1 1.
  • One of the two filter wheels 30, 31 can be equipped with several different long-pass filters and the other of the two filter wheels 30, 31 can be equipped with several different short-pass filters.
  • the filter wheels 30 and 31 can be rotated so that in each case one of the filters can be brought into working position, so that there is an adjustable bandpass filter by a combination of then successively arranged filter.
  • no filter wheels must be provided.
  • a linearly extending long-pass filter 32 may be arranged in front of a linearly extending short-pass filter 33, which are displaceable relative to each other (double arrow P2), so that an adjustable band-pass filter is present (FIG. 12).
  • a linearly-extending segmental filter 34 having a plurality of segments 35 (Fig. 13) each having predetermined filter characteristics (e.g., long, band, or short-pass filters). By positioning the corresponding segment 35, the desired filter characteristic can then be achieved.
  • predetermined filter characteristics e.g., long, band, or short-pass filters
  • a linearly extending gradient filter 36 is provided, which, as indicated by the double arrow P2, can be moved, and thus a predetermined portion in the working area, which is indicated by the bracket 37, is brought.
  • the shifts or rotations described can be done either manually or by motor.
  • the color divider 1 according to the invention is designed as a dual-camera adapter, wherein two cameras 39 and 40 for receiving the first and second images 17, 18 are attached to the color divider 1.
  • the color splitter module 1 with the two cameras 39 and 40 is used for microscopic observation of a multi-colored sample 41 with simultaneous excitation by means of an illumination module 42.
  • the entire device is designed as a microscope and has a main divider 43 and a lens 44.
  • a microscope is thus provided in which two color channels can be detected simultaneously. This avoids the avoidance of image artifacts due to sample movement and / or change during the otherwise customary color channel change.
  • the stress, in particular fast bleaching or phototoxic sensitive samples (especially living cells) can be reduced.
  • the simultaneous detection of two color channels can halve the duration of the experiment, which is particularly important in data-intensive microscopy techniques.
  • FIG. 15 The representation of the microscope in FIG. 15 is to be understood purely by way of example.
  • the microscope can be designed as a light-scanning microscope (for example, a laser scanning microscope).
  • a microscope with the color splitter module 1 according to the invention is also disclosed.
  • Fig. 16 to 18 a further embodiment of the color divider module 1 according to the invention is shown, which is similar in construction to the embodiment of FIG. 5 to 8.
  • the image of the object field 5 is applied to the two image planes 6, 7 by means of a spherically curved mirror 50, which is used twice.
  • the common beam path 14 undergoes a beam path convolution at a first region 51 of the spherically curved mirror 50, so that the pupil 9 is generated.
  • a color divider 53 is arranged, which is not shown in Fig. 16 for simplicity of illustration.
  • the color divider 53 has a color filter 54 arranged in the pupil 9 and a mirror 55 arranged downstream of the color filter 54.
  • the color filter 54 and the mirror 55 are tilted in opposite directions relative to each other, that the first and the second beam path 15, 16 from the color divider 53 in the direction of the spherically curved mirror 50 diverge (here in the z-y plane).
  • both the first and the second beam path 15, 16 meet at a distance from each other in a second region 52 again on the spherically curved mirror 50 and each experience a beam path convolution.
  • the folded beam paths then run to the first and second image plane 6, 7. Since the mirror 50 is spherically curved, there is an image of the object field 5 for the first and second image plane 6, 7 in each case.
  • the optical arrangement corresponds to a 4f structure, wherein the magnification is exactly 1, 0 by the use of the same curved mirror 50 twice.
  • the common beam path 14 when dividing the spherically curved mirror 50 into four imaginary quadrants 21, 22, 23 and 24, lies in the quadrant 22 and 23, the first beam path 15 is in the quadrant 24 and the second beam path 16 is located in the quadrant 21st
  • the region 51 of the spherically curved mirror 50 in which the beam path convolution of the common beam path 14 takes place can also be referred to as the first curved mirror surface
  • the second region 52 of the spherically curved mirror 50 in which the beam path convolutions of the first and second beam paths 15, 16 take place are referred to as the second curved mirror surface.
  • the two curved mirror surfaces 51 and 52 are spaced apart.
  • the locations of the beam path convolutions of the first and second beam paths 15 and 16 are also spaced from each other.
  • the imaging color splitter module 1 is to have a magnification deviating from 1, 0, the spherically curved mirror 50 can be replaced by two mirrors (not shown) for the first and second curved mirror surfaces 51 and 52 be, wherein the two mirrors have different curvatures and thus different focal lengths.
  • the first mirror for the first curved mirror surface 51 in turn generates the pupil 9 and the second mirror for the second curved mirror surface 52 generates the two image planes 6, 7.
  • the second mirror is preferably used for both beam paths and thus for the first and second beam path , so that the magnification is the same for these two beam paths.
  • the spherically curved mirror 50 is used outside its axis, as can be seen in FIGS. 16 and 17.
  • this can lead to unwanted astigmatism in the image.
  • an additional optical element e.g. a tilted plane plate or a tilted spherical lens 56, 57 (each with preferably low refractive power) are inserted.
  • the color filter 54 in the described embodiments of FIGS. 16-19 can be designed in the same way as in FIG. 9 as a color filter coated on the reverse side with a defined wedge-angled substrate and thus monolithic.
  • the imaging color splitter module according to FIGS. 16 to 18 as well as according to FIG. 19 can also be used in a microscope. It can also be combined with the previously described color divider modules. In particular in the manner described in connection with FIG. 4.

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  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
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Abstract

Module séparateur de couleurs imageur comportant une optique d'imagerie (4) pourvue d'une première lentille (2) et d'une seconde lentille (3), pour représenter un champ d'objet (5) dans un premier et un second plan d'image (6, 7), et un séparateur de couleurs (10) qui divise une trajectoire de faisceau (14) commune allant du champ d'objet (5) au séparateur de couleurs (10) en une première trajectoire de faisceau (15) qui s'étend jusqu'au premier plan d'image (6) et en une seconde trajectoire de faisceau (16) qui s'étend jusqu'au second plan d'image (7), la trajectoire de faisceau (14) commune et la première trajectoire de faisceau (15) passant de manière axialement extérieure à travers la première lentille (2) et la seconde trajectoire de faisceau (16) passant de manière axialement extérieure à travers la seconde lentille (3).
PCT/EP2013/056759 2012-04-05 2013-03-28 Module séparateur de couleurs imageur et procédé permettant d'obtenir une image d'un champ d'objet dans un premier plan d'image et dans un second plan d'image WO2013149951A1 (fr)

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DE102012205722.6A DE102012205722B4 (de) 2012-04-05 2012-04-05 Abbildendes Farbteilermodul, Mikroskop mit einem solchen Farbteilermodul sowie Verfahren zum Abbilden eines Objektfeldes in eine erste und eine zweite Bildebene
DE102012205722.6 2012-04-05

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DE102016209524A1 (de) 2016-06-01 2017-12-07 Carl Zeiss Microscopy Gmbh Verlaufsfilteranordnung

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