WO2013133458A1 - Système microfluidique - Google Patents

Système microfluidique Download PDF

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Publication number
WO2013133458A1
WO2013133458A1 PCT/KR2012/001630 KR2012001630W WO2013133458A1 WO 2013133458 A1 WO2013133458 A1 WO 2013133458A1 KR 2012001630 W KR2012001630 W KR 2012001630W WO 2013133458 A1 WO2013133458 A1 WO 2013133458A1
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WO
WIPO (PCT)
Prior art keywords
biological sample
fluid channel
hydrogels
channel
microfluidic system
Prior art date
Application number
PCT/KR2012/001630
Other languages
English (en)
Korean (ko)
Inventor
양광석
전혜경
임귀삼
한정선
Original Assignee
엘지전자 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 엘지전자 주식회사 filed Critical 엘지전자 주식회사
Priority to PCT/KR2012/001630 priority Critical patent/WO2013133458A1/fr
Publication of WO2013133458A1 publication Critical patent/WO2013133458A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3276—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a hybridisation with immobilised receptors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0672—Swellable plugs

Definitions

  • the present invention relates to a microfluidic system, and more particularly, to a microfluidic system capable of reducing the measurement error caused by external factors by capturing the measurement solution to improve the reliability of the measurement value.
  • POCT point of care testing
  • POCT devices for simultaneous multi-item measurement have been accelerated by the development and application of bioMEMS technology.
  • This microfluidic control technique enables the efficient analysis of quantitatively or qualitatively the small amount of specific components contained in biological fluids such as blood. Accordingly, POCT technology is becoming a core technology in the field of biochip or lab-on-a-chip (LOC) technology.
  • LOC lab-on-a-chip
  • microfluidic systems using microfluidic channels have reached a level of sensitivity and reproducibility, even though advances have been made in the development of microchannel manufacturing technology and microquantitative pumps. .
  • the flow path design technology for multi-item measurement is a quantitative distribution of biological samples, quantitative transfer of microfluids, and measurement items. Problems in sensitive areas such as signal interference (interference) between the noise signal, etc. are not completely solved.
  • the present invention has been made in view of the above technical limitations, and an object of the present invention is to provide a microfluidic system capable of multi-item testing using a hydrogel reaction chamber in a single microchannel.
  • Microfluidic system for achieving the above object is a fluid channel for moving a biological sample; And a plurality of hydrogels for capturing the biological sample by a predetermined dose, wherein the hydrogel may cause a volume change when contacted with the biological sample flowing through the fluid channel.
  • the plurality of hydrogels may be spaced apart by a predetermined distance, and the space may include a reaction detector for detecting an electrochemical or optical reaction of the biological sample.
  • the reaction detector may be configured of a biosensor such as a detection electrode.
  • the detection electrode may be immobilized with reactants such as antibodies, DNA, antigens, RNA, receptors, and the like.
  • the spaced plurality of hydrogels may cause a volume change from the hydrogel farthest away from the injection portion of the biological sample.
  • the biological sample may be at least one of blood, urine, serum, and saliva.
  • the hydrogel may cause a volume change when contacted with the biological sample flowing through the fluid channel.
  • a waterproof membrane is formed in the first channel, and when the biological sample flows through the first channel, the waterproof membrane may suppress a reaction between the biological sample and the hydrogel.
  • the plurality of hydrogels may be in physical contact only when the biological sample flows through the second channel, thereby causing a volume change.
  • the biological sample may be injected into the second channel at a portion where the first channel and the second channel are connected while flowing through the first channel to change the flow in the opposite direction.
  • the plurality of hydrogels may capture one of the biological samples by sealing one region of the first channel when the volume change occurs.
  • the plurality of hydrogels may be spaced apart by a predetermined distance, and the space may include a reaction detector for detecting an electrochemical or optical reaction of the biological sample.
  • the reaction detector may be configured of a biosensor such as a detection electrode.
  • the detection electrode may be immobilized with reactants such as antibodies, DNA, antigens, RNA, receptors, and the like.
  • the biological sample may be at least one of blood, urine, serum, and saliva.
  • a multi-item test is possible using a hydrogel reaction chamber in a single microchannel. It can also overcome problems in multiple tests, such as signal interference between measurement items.
  • FIG. 1 is a view for explaining the basic configuration and principle of a microfluidic system according to an embodiment of the present invention
  • FIG. 2 is a view showing the configuration of a microfluidic system according to an embodiment of the present invention
  • 3A to 3C are diagrams sequentially showing operations of a microfluidic system according to an embodiment of the present invention.
  • FIG. 5 is a view showing the configuration of a microfluidic system according to another embodiment of the present invention.
  • FIG. 1 is a view for explaining the basic configuration and principle of a microfluidic system according to an embodiment of the present invention.
  • the biological sample 10 is injected through the inlet 20 and flows through the channel. Between the injection port 20 and the discharge port 30, the biological sample 10 reacts with the reactants in the region 50 provided with the electrode, the electrode is sensed to examine the components in the biological sample 10.
  • the biological sample 10 refers to a component that can be extracted from a human body such as blood, urine, serum, and saliva, and the electrode includes an antibody, DNA, antigen, RNA, or receptor.
  • a reactant such as a receptor is fixed and the components of the biological sample 10 are analyzed.
  • the microfluidic system according to an exemplary embodiment of the present invention moves or diffuses a biological sample generated by internal or external factors during measurement when the biological sample is to be detected electrochemically or optically. Or to prevent volume changes and ultimately improve the reliability of the values measured from the system.
  • the microfluidic system includes a fluid channel 100 and a reaction detector 120 for moving the biological sample 10. Meanwhile, hydrogels 110-1 and 110-2 having a function of capturing the moving biological sample 10 are included.
  • Fluid channel 100 may be manufactured in a very small form of micro size.
  • the reaction detector 120 may assume an electrode as an electrochemical measurement unit, but may be configured as various biosensors. However, for convenience of description, the following description will be made assuming the detection electrode.
  • the hydrogels 110-1 and 110-2 are designed to cause cross-sectional area changes inside the fluid channel 100 by absorbing moisture. Through this, the biological sample 10 flowing through the fluid channel 100 has an effect of being physically or chemically blocked between the hydrogels 110-1 and 110-2 for a predetermined time.
  • 3A-3C illustrate the operation of the microfluidic system in order according to an embodiment of the present invention.
  • the biological sample 10 flows through the fluid channel 100.
  • the electrode 120 and the hydrogels 110-1 and 110-2 exist, and the electrode 120 has the first hydrogel 110-1 and the second hydrogel 110 spaced apart from each other. It is located between -2).
  • the hydrogels 110-1 and 110-2 are formed of the biological sample 10 and the water.
  • the hydrogels 110-1 and 110-2 are formed of the biological sample 10 and the water.
  • a volume change by changing the components of the first hydrogel (110-1) and the second hydrogel (110-2) it is possible to vary the rate causing the volume change.
  • the hydrogels 110-1 and 110-2 which caused the volume change as shown in FIG. 3C, block the passage of the fluid channel 100, and the biological sample 10 includes the first hydrogel 110-1 and the second hydrogel 110-. 2) is captured between.
  • the reactant fixed to the electrode 120 reacts with the biological sample 10, and the electrode 120 may sense the reaction to analyze the biological sample 10.
  • FIGS. 4A to 4C are diagrams illustrating a microfluidic system according to another embodiment of the present invention.
  • the microfluidic system of FIGS. 4A to 4C has two fluid channels, that is, the first fluid channel 100 and the second fluid channel 130.
  • the first fluid channel 100 is characterized in that the waterproof membrane 140 is formed, which is the hydrogels 110-1 and 110-2 while the biological sample 10 flows through the first fluid channel 100. It serves to suppress the reaction.
  • the hydrogels 110-1 and 110-2 do not react with the biological sample 10 to cause a volume change.
  • the biological sample 10 passes the second hydrogel 110-2 as shown in FIG. 4B, the biological sample 10 flows through the second fluid channel 130 connected to the first fluid channel 100. Since the hydrogels 110-1 and 110-2 are in contact with the first fluid channel 100 and the second fluid channel 130, when the biological sample flows in the second fluid channel 100, first, the second hydrogel 110 may be used. -2) reacts and then the first hydrogel (110-1) is reacted.
  • the biological sample 10 is captured after sufficient flow, and thus a desired amount can be obtained.
  • FIG. 5 is a view showing the configuration of a microfluidic system according to another embodiment of the present invention.
  • the difference from the above two embodiments is the use of a larger number of hydrogels (110-1,110-2,110-3,110-4) rather than two hydrogels.
  • the operation method is the same as in the above two embodiments. That is, the biological sample 10 comes into contact with the hydrogels 110-1, 110-2, 110-3, and 110-4 while moving the fluid channel 100, and the hydrogels 110-1, 110-2, 110-3, and 110-4 undergo volume changes. It raises and captures the biological sample 10. In this case, however, it is divided into a plurality of sections and captured, so that each section may have a different reactant to perform various tests at the same time. In this case, by varying the reaction rate of each of the hydrogels 110-1, 110-2, 110-3, and 110-4, sufficient biological samples 10 can be captured.
  • microfluidic system of FIG. 5 may be configured as a dual channel as shown in FIGS. 4A to 4C. If so, the fourth hydrogel (110-4) to the first hydrogel (110-1) causes a change in volume in order to be able to sufficiently capture the biological sample (10).

Abstract

La présente invention concerne un système microfluidique. Le système microfluidique de l'invention comprend : un canal pour fluide dans lequel un échantillon biologique peut s'écouler ; et une pluralité d'hydrogels servant à capturer une quantité prédéterminée de l'échantillon biologique. Les hydrogels peuvent provoquer une variation volumétrique lors de leur contact avec l'échantillon biologique s'écoulant dans le canal pour fluide. Un test multi-objet peut ainsi être réalisé au moyen d'une chambre réactionnelle à base d'hydrogel se trouvant dans un seul microcanal. En outre, les problèmes liés aux tests multi-objets, tels que les interférences des signaux entre les objets à mesurer, peuvent être résolus.
PCT/KR2012/001630 2012-03-06 2012-03-06 Système microfluidique WO2013133458A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/KR2012/001630 WO2013133458A1 (fr) 2012-03-06 2012-03-06 Système microfluidique

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR2012/001630 WO2013133458A1 (fr) 2012-03-06 2012-03-06 Système microfluidique

Publications (1)

Publication Number Publication Date
WO2013133458A1 true WO2013133458A1 (fr) 2013-09-12

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WO (1) WO2013133458A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050028607A (ko) * 2003-09-19 2005-03-23 학교법인 포항공과대학교 공기 경계면을 이용한 미세 채널에서의 미세유체흐름조절용 칩의 제조 방법과 미세유체 폭 조절방법
KR100695272B1 (ko) * 2006-01-10 2007-03-14 단국대학교 산학협력단 이종 식물 세포융합장치
KR20080027392A (ko) * 2005-07-14 2008-03-26 나노디텍 코포레이션 미세유체장치와 미세유체장치의 제작 및 사용방법
KR20100071217A (ko) * 2008-12-19 2010-06-29 한국전기연구원 모세관 밸브가 장착된 랩온어칩 및 랩온어칩용 모세관 밸브의 제조 방법.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050028607A (ko) * 2003-09-19 2005-03-23 학교법인 포항공과대학교 공기 경계면을 이용한 미세 채널에서의 미세유체흐름조절용 칩의 제조 방법과 미세유체 폭 조절방법
KR20080027392A (ko) * 2005-07-14 2008-03-26 나노디텍 코포레이션 미세유체장치와 미세유체장치의 제작 및 사용방법
KR100695272B1 (ko) * 2006-01-10 2007-03-14 단국대학교 산학협력단 이종 식물 세포융합장치
KR20100071217A (ko) * 2008-12-19 2010-06-29 한국전기연구원 모세관 밸브가 장착된 랩온어칩 및 랩온어칩용 모세관 밸브의 제조 방법.

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Title
EDDINGTON, D. T. ET AL.: "Flow control with hydrogels", ADV. DRUG DELIV. REV., vol. 56, no. 2, 10 February 2004 (2004-02-10), pages 199 - 210, XP055105833, DOI: doi:10.1016/j.addr.2003.08.013 *

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