WO2013132347A3 - Improved elisa immunoassay for calprotectin - Google Patents
Improved elisa immunoassay for calprotectin Download PDFInfo
- Publication number
- WO2013132347A3 WO2013132347A3 PCT/IB2013/000886 IB2013000886W WO2013132347A3 WO 2013132347 A3 WO2013132347 A3 WO 2013132347A3 IB 2013000886 W IB2013000886 W IB 2013000886W WO 2013132347 A3 WO2013132347 A3 WO 2013132347A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- antibody
- calprotectin
- solid support
- monoclonal anti
- labeled polyclonal
- Prior art date
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4727—Calcium binding proteins, e.g. calmodulin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/06—Gastro-intestinal diseases
- G01N2800/065—Bowel diseases, e.g. Crohn, ulcerative colitis, IBS
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
An improved immunoassay for S100A9 provides increased reproducibility and accuracy for the determination of calprotectin, allowing more accurate diagnosis and screening for inflammatory bowel disease. In particular, the immunoassay is a sandwich immunoassay that uses an unlabeled monoclonal anti-S100A9 antibody as the capture antibody and a labeled polyclonal anti-calprotectin antibody as the detection antibody. Further improvements in reproducibility and accuracy are obtained by using citrate buffer as a coating buffer to coat a solid support, such as used in an ELISA assay, with the capture antibody. In general, the method comprises the steps of: (1 ) providing a monoclonal anti-S100A9 antibody; (2) coating the monoclonal anti-S100A9 antibody onto a solid support; (c) reacting the solid support with coated monoclonal anti-S100A9 antibody with a sample that may contain calprotectin to bind any calprotectin in the sample to the solid support; (d) washing the solid support; (e) reacting the solid support with a labeled polyclonal anti-calprotectin antibody to bind the labeled polyclonal anti-calprotectin antibody to the calprotectin bound to the solid support, the labeled polyclonal antibody being able to produce a detectable signal proportional to the concentration of the antibody bound to the calprotectin bound to the solid support; and (f) measuring the detectable signal to determine the concentration of calprotectin in the sample. The invention further includes diagnostic methods, methods of screening antibodies, and kits.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US201261607131P | 2012-03-06 | 2012-03-06 | |
US61/607,131 | 2012-03-06 |
Publications (2)
Publication Number | Publication Date |
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WO2013132347A2 WO2013132347A2 (en) | 2013-09-12 |
WO2013132347A3 true WO2013132347A3 (en) | 2014-03-06 |
Family
ID=48626472
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2013/000886 WO2013132347A2 (en) | 2012-03-06 | 2013-03-05 | Improved elisa immunoassay for calprotectin |
Country Status (1)
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WO (1) | WO2013132347A2 (en) |
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BR112017010268B1 (en) | 2014-11-19 | 2024-01-16 | P & M Venge Ab | BINDING AGENT, DIAGNOSTIC COMPOSITION, DIAGNOSTIC KIT, METHOD OF DIAGNOSING A BACTERIAL INFECTION OR OF DIFFERENTIATION BETWEEN A BACTERIAL INFECTION AND A VIRAL INFECTION, METHODS FOR RULED OUT A BACTERIAL OR VIRAL INFECTION IN AN INDIVIDUAL, METHODS FOR CONSIDERING AN INFECTION BACTERIAL ACTION OR VIRAL IN AN INDIVIDUAL, METHOD FOR DISTINGUISHING BETWEEN A BACTERIAL OR MIXED INFECTION AND A VIRAL INFECTION IN AN INDIVIDUAL, METHOD FOR RULING OUT AN INFECTIOUS DISEASE, METHOD FOR IDENTIFYING THE TYPE OF INFECTION AND DEVICE FOR DIAGNOSING BACTERIAL INFECTIONS |
US20180017576A1 (en) * | 2015-01-23 | 2018-01-18 | Westfaelische Wilhelms-Universitaet Muenster | Compounds and methods for the detection of calprotectin |
CN104597255B (en) * | 2015-02-04 | 2017-07-21 | 华中农业大学 | The test card and preparation method and application of pseudorabies virus gE protein antibodies in a kind of detection Swine serum |
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CN105181977A (en) * | 2015-11-05 | 2015-12-23 | 四川沃文特生物技术有限公司 | Kit for detecting calprotectin in excrement |
CA3007098A1 (en) | 2015-12-22 | 2017-06-29 | Amgen Inc | Ccl20 as a predictor of clinical response to il23-antagonists |
EP3486654B1 (en) * | 2016-07-13 | 2021-08-18 | Sekisui Medical Co., Ltd. | Detection method using immunochromatography |
US11267854B2 (en) | 2016-07-20 | 2022-03-08 | Westfaelische Wilhelms-Universitaet Muenster | Complex-specific standardization of immunological methods for the quantification of S100A12 |
ES2948232T3 (en) | 2017-02-07 | 2023-09-06 | Lyfstone B V | Biomarkers to diagnose implant-related implant revision risk due to aseptic loosening |
CN111094978A (en) | 2017-05-09 | 2020-05-01 | 免疫诊断股份公司 | Method for determining members of calcium binding protein S100 family by immunoturbidimetry |
US20200200760A1 (en) * | 2017-05-16 | 2020-06-25 | Lankenau Institute Of Medical Research | Compositions comprising ligands to rhob protein and the uses thereof |
CN108196066A (en) * | 2018-01-22 | 2018-06-22 | 安徽伊普诺康生物技术股份有限公司 | A kind of kit for measuring calcium sozin and its preparation application method |
CN109856403A (en) * | 2018-12-21 | 2019-06-07 | 河北省医疗器械与药品包装材料检验研究院(河北省医疗器械技术审评中心) | A kind of S100- β albumen time-resolved fluoroimmunoassay chromatography detection kit |
US20220163525A1 (en) * | 2019-02-15 | 2022-05-26 | Immundiagnostik Ag | Rapid test for diagnosis of bacterial infections in neonates |
CN110488025A (en) * | 2019-09-24 | 2019-11-22 | 嘉兴行健生物科技有限公司 | A kind of chemiluminescence quantitative detection excrement calprotectin and its detection method and its intestinal health detection purposes |
CN112578125B (en) * | 2019-09-27 | 2022-10-25 | 成都中医药大学 | Application of reagent for detecting content of calprotectin in preparation of ovarian lesion screening kit |
CN110609143A (en) * | 2019-10-31 | 2019-12-24 | 苏州普瑞森基因科技有限公司 | Calprotectin heterodimer detection kit and application thereof |
CN110850097A (en) * | 2019-11-07 | 2020-02-28 | 江苏宜偌维盛生物技术有限公司 | Calprotectin detection kit and detection method |
CN111273029B (en) * | 2020-02-25 | 2023-03-31 | 芜湖天明生物技术有限公司 | rhTSG-6 double-antibody sandwich ELISA quantitative detection kit and use method and application thereof |
CN114891103B (en) * | 2022-06-11 | 2023-07-07 | 巴迪泰(青岛)生物科技有限公司 | Calprotectin monoclonal antibody and kit |
CN115856290A (en) * | 2022-12-15 | 2023-03-28 | 无锡市第二人民医院 | Fluorescent immune quantitative test strip for detecting calcium-binding protein A9, and detection method and application thereof |
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