WO2013122302A1 - Method for fixing strontium and composition for same - Google Patents

Abstract

The present invention relates to a method for biologically fixing the dissolved strontium in contaminated water by means of rhodolith-derived microorganisms. The present invention provides a method for fixing the dissolved strontium in contaminated water, comprising: a step of enrichment culturing the rhodolith-derived microorganisms; and a step of treating the contaminated water with the enrichment cultured microorganisms to enable the microorganisms to react with the contaminated water, thus fixing the dissolved strontium in the contaminated water as a strontium carbonate mineral. The method for biologically fixing the strontium according to the present invention is advantageous in that it employs more simple and eco-friendly processes as compared to technologies for chemically fixing strontium. The method of the present invention fixes strontium at a high rate at room temperature, and is thus sufficient to be applied to an industrial field. The method of the present invention enables strontium to be finally fixed in the form of strontianite, and thus is effective in terms of recycling of resources.

Description

Strontium fixation method and composition for same

The present invention relates to a method for fixing strontium and a composition therefor, and more particularly, to a method for biologically fixing dissolved strontium in contaminated water using microorganisms derived from red blood nodules and a composition therefor.

In addition to the problem of global warming, after the recent nuclear power plant accidents in Japan, environmental pollution by radioactive materials and human pollution has become a serious social problem. Radioactive strontium is a radionuclide that can be released into the environment due to nuclear weapon testing and nuclear power depletion. More than 90% of the total is deposited on the hydroxyapatite structure of bone and acts on the DNA of bone and bone marrow cells for a long time. It is known to be a radiotoxic radionuclide that causes dizziness and leukemia.

As the level of the Japanese nuclear accident rises to the seventh worst level, such as the Chernobyl crisis, radiological debris that appeared in Chernobyl is appearing in the soil and plants around Fukushima. Due to a nuclear accident in Japan, two soil samples from Namie-cho, Fukushima Prefecture and one point from Itate-son were analyzed. As a result, strontium-90 detected 32 becquerels per kg of soil and had a half-life of 50 days. For 89, up to 260 becquerels were detected. In the past 40 years, many scientists from developed countries have been steadily researching medical methods such as preventive medicines for radiation disorders, excretion accelerators for radioactive isotopes, and treatments for recovery from disorders. It is true.

When the nucleus element is in an ionic state, it easily flows into the groundwater in the form of anions or cations, and some microorganisms cause a reaction to reduce and precipitate or adsorb ionized metal ions, and to deposit the metal ions under the ground water in solid form. Precipitate. Academia studying the treatment of high-level nuclear waste using these microorganisms has been evaluated to play an important role in preventing the spread of high-level nuclear materials stored underground for hundreds of thousands of years. Although studies have been conducted to sequester carbon dioxide and metal ions using coal ash, few studies have been conducted on the fixation of strontium in an ionic state using microorganisms.

It is an object of the present invention to provide an efficient strontium fixation method with increased reaction rate and a composition therefor.

In order to achieve the above object, one aspect of the present invention comprises the steps of enrichment culture of red blood cells nodules; And treating the concentrated cultured microorganisms in contaminated water and reacting the same, thereby fixing the dissolved strontium in the contaminated water with strontium carbonate minerals.

In addition, in order to achieve the above object, another aspect of the present invention comprises a concentrated culture of the microorganisms derived from red algae as an active ingredient, and dissolved strontium fixing composition in contaminated water for fixing the dissolved strontium in contaminated water with strontium carbonate mineral to provide.

Biological strontium immobilization method of the present invention has the advantage that the fixing process is simple and environmentally friendly compared to chemical strontium immobilization technology. Further, the effectiveness in terms of recycling of resources, because it is possible to immobilize the strontium rapidly even at room temperature is sufficient to be applied to the industrial field, strontium are to be finally fixed in the form of strontium thiazol nitro (strontianite, SrCO ₃₎ Have

However, the effects of the present invention are not limited to the above-mentioned effects, and other effects not mentioned will be clearly understood by those skilled in the art from the following description.

FIG. 1 is a photograph of red nodules collected from Seogwang-ri coast of Udo, Bukjeju-gun, Jeju Island.

2 is a graph showing the results of X-ray diffraction analysis (X-ray diffraction, XRD) of the red nodules collected from the Seogwangni coast of Udo, Jeju Island, North Jeju Island.

Figure 3 is a phylogenetic diagram showing the results of 16S rRNA-DGGE analysis of the microorganisms present in the enrichment culture of the blush-derived microorganisms.

Figure 4 is a photograph showing the process and the result of the production of strontium carbonate mineral by the enrichment culture of the microorganisms derived from blush nodules.

Figure 5 is a graph showing the results of XRD analysis of the strontium carbonate mineral produced by the enrichment culture of the blush-derived microorganisms.

FIG. 6 is a graph and a graph showing the results of SEM-EDS analysis of strontium carbonate minerals produced by the enrichment culture of red blood cell nodules.

Hereinafter, the present invention will be described in detail.

One. Method of Fixing Strontium

One aspect of the present invention comprises the steps of enriching cultures of the algae-derived microbes; And fixing the dissolved strontium in the contaminated water with strontium carbonate mineral strontianite (SrCO ₃ ) by treating and reacting the concentrated cultured microorganisms with contaminated water. To provide.

Method for fixing the dissolved strontium in contaminated water of the present invention comprises the steps of: 1) enrichment culture of the erythroid-derived microorganisms; And 2) fixing the dissolved strontium in the contaminated water with strontium carbonate minerals by treating and reacting the microorganism enriched in step 1) with contaminated water.

Thickening culture of the blush-derived microorganisms of step 1) is the step of collecting 1 ') flushed nodules; And 2 ') the red nodule collected in step 1') was treated with D-1 medium (Sanchez-Roman et al., 2009 / Sanchez-Roman et al., 2011), MH (Mueller-Hinton) medium (Rodroguez-Valera). et al., 1981) and Saline medium (Chahal et al., 2011) is carried out by a culture method comprising the step of culturing in any one medium selected from the group consisting of aerobic environment. The composition of the medium as described above is shown in Table 1 below.

Table 1

Composition of D-1 Medium		Composition of MH Medium		Composition of Saline Badge
ingredient	content	ingredient	content	ingredient	content
yeast extract	1.0%	yeast extract	1.0%	Nutrient broth	3 g / L
proteose peptone	0.5%	proteose peptone	0.5%	NH 4 Cl	10 g / L
glucose	0.1%	glucose	0.1%	NaHCO 3	2.12 g / L
NaCl	3.5%	NaCl	2.5-20%	NaCl	0.85%
				CaCl2 · 2H2O	25 g / L

The red nodule of step 1 ') is a collection of autogenous minerals formed by precipitation of calcium carbonate, which is the main component of calcite, by red algae growing while calcite, which is a carbonate mineral, is precipitated in cells or between cell walls. In other words, the red nod means a nodule formed by precipitation of calcium carbonate by red algae. The calcium carbonate constituting the red nodules is precipitated by the biological metabolism of the red algae, which is derived from carbonate minerals such as calcite which are present around the red algae. The carbonate mineral may be produced by a variety of causes, one of the causes may be generated by the metabolism of carbonate mineral-producing microorganisms, the microorganisms that produce such a carbonate mineral may be present in the flushing nodules. Therefore, the redness nodule used in step 1 ') is a source of the carbonate-generating microorganisms.

The flushing nodules of step 1 ') include carbonate mineral producing microorganisms. The carbonate mineral-producing microorganism is Proteus mirabilis , Marinobacterium coralalii , which combines dissolved metal cations in water, more preferably strontium cations, with carbonate anions or hydrogen carbonate anions to form carbonate minerals during metabolism. ( Marinobacterium coralli ), including a variety of microorganisms that contribute to the formation of carbonate minerals inhabiting blush nodules.

It is preferable that the red nodule of step 1 ') is collected to have a size of 3 cm to 10 cm, and the red nodule is more preferably collected to have a size of 5 cm to 7 cm, but is not limited thereto. If the size of the flushing nodules is less than 3 cm, there is a problem that the absolute number of carbonate-producing microorganisms is less. If the size of the flushing nodules is greater than 10 cm, the number of carbonate-producing microorganisms present per unit area of the flushing nodules, that is, the production of carbonates Since there is a problem that the density of the microorganism is lowered, there is a problem in that it takes a long time to enrich the microorganism in the step 2 '). In a specific embodiment of the present invention, carbonate mineral-producing microorganisms were enriched using red algae nodules collected from 5 cm to 7 cm in size from Seogwang-ri coast in Udo, Bukjeju-gun, Jeju Island (see FIG. 2).

The medium of step 2 ') may be used without limitation as long as it is a medium used for enriching culture of carbonate-producing microorganisms, but D-1 medium, MH medium, Saline medium, and the like may be used. Such a medium is suitable for the growth of microorganisms because it contains yeast extract and proteose peptone or nutrient broth necessary for the growth of carbonate-producing microorganisms. It is suitable for growing microbes in the ocean.

It is preferable that calcium cation, magnesium cation or strontium cation is added to the medium of step 2 '). In order to enrich and culture microorganisms involved in the production of carbonate minerals among the microorganisms present in the red algae, calcium cations, magnesium cations, or strontium cations which are used to fix CO ₃ ^2- as carbonate minerals during biological metabolism. It is preferable to add the same cation to the medium. The addition of the calcium cation, magnesium cation or strontium cation is preferably added calcium salt, magnesium salt or strontium salt, and the addition of the calcium cation, magnesium cation or strontium cation is composed of calcium acetate, magnesium acetate and strontium acetate. It is more preferred to add any one or two or more mixtures selected from the group, but is not limited thereto.

Cultivation of step 2 ') is performed in an aerobic environment similar to the marine environment in which the red nodules are present. Red algae nodules derived from the island of Udo, Jeju Island, which are used in specific embodiments of the present invention, are mainly formed in the shallow water environment of the main islands, and the red algae have been reported to be formed by red algae. However, the mechanism by which the material acting as the nucleus forming the redness nodule is not known. Accordingly, the present inventors determined that the microorganisms present in the red nodules could form calcium carbonate that could serve as the first nucleus in the formation of the red nodules. Therefore, aerobic conditions similar to those of the local environment where red nodules were formed were formed to enrich and culture microorganisms in the red nodules.

In a specific embodiment of the present invention, after the red algae collected from the Seogwang-ri coast of Udo, Jeju-do, Jeju-do, pulverized using Agate and Mortar, about 5 g of the red algae nodules were placed in D-1 medium. After injecting a mixture of calcium acetate and magnesium acetate, the culture was cultured under aerobic conditions for 7 days to obtain a thickening culture of microorganisms derived from red algae.

Thickening the cultured microorganism is Vibrio find fun tea kusu (Vibrio alginolyticus) of said step 2), Vibrio Owen Shi (Vibrio owensii), Vibrio Cui (Vibrio xuii), Vibrio block nipi kusu (Vibrio vulnificus), Vibrio flat ruby Alice (Vibrio fluvialis), Vibrio four races (Vibrio nereis), Proteus Mira Billy's (Proteus mirabilis), Marino tumefaciens Coral riyi (Marinobacterium coralli), Bago Caucus platform Ruby Alice (Vagococcus fluvialis), Puerto earthy Te Solarium PERE Four Martens (Fusobacterium perfoetens , Tindallia californiensis , Arcobacter marinus , Parabacteroides gordonii , and Prolixibacter bellariivorans . It may include any one or more microorganisms selected, the microorganism is Vibrio alginolitis ( Vib rio alginolyticus , Vibrio owensii , Vibrio xuii , Vibrio vulnificus , Vibrio fluvialis , Vibrio nereis , Proteus mirabilis Proteus mirabilis ) and Marinobacterium coralli ( Marinobacterium coralli ) more preferably include any one or more selected from the group consisting of, but not limited to. In a specific embodiment of the present invention, 16S rRNA-DGGE analysis was carried out to confirm the diversity of the iron-reducing microorganisms present in the enrichment culture of the blush-derived microorganisms, Proteus mirabilis and Marinobacterium coral. Various species of carbonate mineral precipitation microorganisms, including Lii ( Marinobacterium coralli ), have been identified (see Figure 3).

The contaminated water of step 2) means water in which strontium is excessively caused by artificial industrial activity, and is seawater or fresh water in which strontium to be fixed is dissolved. Strontium to be fixed may be dissolved and present in the form of strontium cations (Sr ²⁺ ) in the seawater or fresh water. The contaminated water may have various anions in addition to strontium. In particular, the anion may be a carbonate anion (CO ₃ ^2- ) or a hydrogen carbonate anion (HCO ₃ ⁻ ), but is not limited thereto. The carbonate anion or hydrogen carbonate anion binds to the strontium cation present in the contaminated water and is fixed in the form of strontium carbonate mineral strontianite. In the present invention, the binding of the anion and the cation is biological Promoted by metabolism

The treatment of step 2) is to inject the microorganism enriched in the flushing nodules derived in step 1) into the contaminated water, the enrichment culture of the microorganisms derived from the blush nodules is 0.5 w / v relative to the total volume of the contaminated water It is preferred to inject at a concentration of% to 1.5 w / v%. When the thickening culture is injected at a concentration lower than 0.5 w / v% with respect to the total volume of the contaminated water, there is a problem that it takes a long time to fix the dissolved strontium in the contaminated water. In addition, when the thickening culture is injected at a concentration higher than 1.5 w / v% relative to the total volume of the contaminated water, the concentration of dissolved oxygen is lowered by the respiration of excess microorganisms in the thickening culture, contaminated water becomes an anaerobic environment, In the end there is a problem of corruption. Therefore, it is very important to appropriately control the amount of microorganisms treated in contaminated water, and it is necessary to appropriately control the concentration of the thickening culture.

The treatment of step 2) may be performed in addition to the concentrated culture of step 1) in addition to the concentrated culture of the step 1) of the carbonate anion (CO ₃ ^2- ) or hydrogen carbonate anion (HCO ₃ ⁻ ). In particular, when the concentration of carbonate anion or hydrogen carbonate anion in the contaminated water is low, the carbonate anion (CO ₃ ^2- ) or hydrogen carbonate anion in order to make this more smooth since the strontium cation in the contaminated water is not easily fixed to the carbonate mineral which will supply the ^- (HCO _3). The supply of the carbonate anion or the hydrogen carbonate anion is preferably performed by treating carbonate or hydrogen carbonate, but is not limited thereto.

The reaction of step 2) is performed by the microorganisms in which the blush-derived microorganisms in the thickening culture of step 1) are injected into the contaminated water to perform biological metabolism, thereby dissolving the dissolved strontium in the contaminated water in the form of strontium carbonate mineral strontium carbonate. As a process of fixation, since the blush-derived microorganisms in the thickening culture are aerobic microorganisms, it is preferable to proceed under aerobic conditions in which oxygen is sufficiently supplied. In other words, the microorganisms derived from the blush nodules in the thickening culture form strontium carbonate mineral strontite by combining dissolved strontium cations with carbonate anions or hydrogen carbonate anions through biological metabolism, and in water in the form of strotianite Secure dissolved strontium. In addition, the reaction is preferably carried out for 7 days at room temperature, atmospheric pressure conditions. The reaction is fixed to carbonate at a faster rate through the complex interaction of various microorganisms contained in the thickening culture.

The carbonate mineral of step 2) may be strontium carbonate mineral strontianite (SrCO ₃ ), but when the concentration of calcium cations or magnesium cations is higher than that of strontium cations in the contaminated water, calcium carbonate or carbonate A calcite composed of magnesium forms carbonate minerals.

In a preferred embodiment of the present invention, 1.0 w / v% of the blush nodules microbial enrichment culture was added to the contaminated water with 100 mmol of strontium-acetate and incubated for 7 days, after 4 days in the Erlenmeyer flask As the turbidity increased, white precipitates began to form, and it was confirmed that a considerable amount of precipitates were observed after 7 days (see FIG. 4). In addition, XRD analysis of the precipitate, the peak of the d (104) was determined to be strotianite measured by 3.52 kPa (see Fig. 5), SEM-EDS analysis, the precipitate is a spherical strontite Observation was possible (see FIG. 6).

2. Composition for Fixing Strontium

Another aspect of the present invention provides a composition for fixation of dissolved strontium in contaminated water comprising a concentrated culture of microorganisms derived from red blood nodule as an active ingredient, and fixing dissolved strontium in contaminated water in the form of strontium carbonate mineral strontiumite. do.

Dissolved strontium immobilization composition in contaminated water of the present invention comprises a thickening culture of the microorganisms derived from blush nodule as an active ingredient.

The composition for fixing the dissolved strontium in the contaminated water is a composition used in the strontium fixing method described in detail in the " 1. Strontium fixing method". Therefore, the description of the method of using the composition uses the description of " 1. How to fix the strontium ", and will be described below only the other properties of the composition of the present invention.

Flushing nodule used in the preparation of the composition is the same as described in detail in " 1. Method of Fixing Strontium ". Therefore, the red algae used in the preparation of the composition also includes carbonate mineral producing microorganisms, and the red algae-derived microorganisms are proteus which combines dissolved metal cations in water with carbonate anions or hydrogen carbonate anions to form carbonate minerals during metabolism. It refers to a variety of microorganisms that contribute to the formation of carbonate minerals while inhabiting red nodules including Proteus mirabilis and Marinobacterium coralli .

* The thickening culture can be prepared by the thickening culture method of the microorganisms derived from the blush nodules described in detail in the " 1. strontium fixing method ", more preferably the steps 1 ') and 2') It can be prepared by a thickening culture method comprising. The concentrated culture is brioche find fun tea kusu (Vibrio alginolyticus), Vibrio Owen Shi (Vibrio owensii), Vibrio Cui (Vibrio xuii), Vibrio block nipi kusu (Vibrio vulnificus), Vibrio flat ruby Alice (Vibrio fluvialis), Vibrio Yes Vibrio nereis , Proteus mirabilis , Marinobacterium coralli , Vagococcus fluvialis , Fusobacterium perfoetens , Tyndalia One or more selected from the group consisting of Tindallia californiensis , Arcobacter marinus , Parabacteroides gordonii , and Prolixibacter bellariivorans It may include a microorganism, but is not limited thereto. In a specific embodiment of the present invention, 16S rRNA-DGGE analysis was carried out to confirm the diversity of the iron-reducing microorganisms present in the enrichment culture of the blush-derived microorganisms, Proteus mirabilis and Marinobacterium coral. Microorganisms of various species have been identified including Lii ( Marinobacterium coralli ) (see Figure 3).

The composition may further include a carbonate anion (CO ₃ ^2- ) or a hydrogen carbonate anion (HCO ₃ ⁻ ) in the thickening culture of the microorganism derived from the red blood nodules. In particular, when the concentration of carbonate anion or hydrogen carbonate anion in the contaminated water is low, the carbonate anion (CO ₃ ^2- ) or hydrogen carbonate anion in order to make this more smooth since the strontium cation in the contaminated water is not easily fixed to the carbonate mineral As (HCO ₃ ⁻ ) is supplied, it may be included by adding carbonate or hydrogen carbonate to the composition.

The contaminated water refers to water in which strontium metal is excessively caused by artificial industrial activity, and is seawater or fresh water in which strontium to be fixed is dissolved. Strontium to be fixed may be dissolved and present in the form of strontium cations (Sr ²⁺ ) in the seawater or fresh water. The contaminated water may have various anions in addition to strontium. In particular, the anion may be a carbonate anion (CO ₃ ^2- ) or a hydrogen carbonate anion (HCO ₃ ⁻ ), but is not limited thereto. The carbonate anion or hydrogen carbonate anion binds to the strontium cation present in the contaminated water and is fixed in the form of strontium carbonate mineral strontianite (SrCO ₃ ), in which the binding of the anion and the cation is flushed. It is promoted by the biological metabolism of nodules-derived microorganisms. Various microorganisms in the thickening culture included in the composition can more effectively promote the binding of the anion and cation through a complex interaction.

When the composition is treated in the contaminated water, the microorganisms derived from the blush nodules in the thickening cultures contained in the composition undergo biological metabolism in the contaminated water to fix dissolved strontium in the contaminated water in the form of strontium carbonate minerals, The strontium carbonate mineral may be strontianite.

In a preferred embodiment of the present invention, 1.0 w / v% of the blush nodules microbial enrichment culture was added to the contaminated water with 100 mmol of strontium-acetate and incubated for 7 days, after 4 days in the Erlenmeyer flask As the turbidity increased, white precipitates began to form, and it was confirmed that a considerable amount of precipitates were observed after 7 days (see FIG. 4). In addition, XRD analysis of the precipitate, the peak of the d (104) was determined to be strotianite measured by 3.52 kPa (see Fig. 5), SEM-EDS analysis, the precipitate is a spherical strontite Observation was possible (see FIG. 6).

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are only for better understanding of the present invention, and the present invention is not limited to the following examples.

Example 1

Collection and Analysis of Red Nodules

<1-1> Characterization of Seawater around Udo Island

In order to investigate the chemical characteristics of seawater at each beach in Udo, Jeju Island, pH was measured and inductively coupled plasma-atomic emission spectrometry (ICP-AES) analysis was performed. The pH of seawater was measured by using an Orion pH meter after collecting about 50 ml of seawater sample. The pH meter was calibrated after measuring the pH in a solution of pH 4, 7, 10, respectively, to measure the pH of seawater. In order to measure the amount of cations present in seawater, 1 ml of nitric acid was added to prevent the formation of sediments after seawater samples were collected.

As a result of the pH measurement, the seawater around Jeju Island's Udo Island was found to be between about acidic and neutral at an average of 5 to 8 (Table 2). In addition, as a result of ICP-AES analysis, the concentration of calcium ions (Ca ²⁺ ) in the seawater around Jeju-do was about 400 mg / l (Table 3).

In general the carbonate minerals in the calcite (CaCO ₃₎ is a basic environment (pH = 9.3 to 9.8) and high saturation (Saturation Index = 1 to 1.4) (degree of saturation is, Ca and CO ₃ ^2- concentrations higher chemically CaCO ₃ of Considering that it is chemically formed at room temperature conditions, it is known that redness nodules found at Seogwang-ri beach in West Seogwi-ri, Jeju Island, were formed by other causes than chemical action. Able to know.

TABLE 2

PH measurement results of seawater around Jeju Island, Udo
location	pH
Beetle Beach (East)	7
Seogwang-ri Beach (West)	8
Dolkan Beach (South)	7
Hago manual beach (north)	5

TABLE 3

ICP-AES measurement result of seawater around Udo, Jeju Island
Unit: mg / l
element	Beetle Beach (East)	Seogwang-ri Beach (West)	Hago manual beach (north)
Si	<0.01	0.34	<0.01
Sr	6.25	6.25	6.31
Ca	406.7	396.1	405.8
Mg	1,264	1,225	1,239
K	393.2	380.3	378.7
Na	10340	10240	10270
S	877.5	873.6	910.7
Cl	21550	19540	19970

<1-2> Collection of Flush Nodules

The red algae of the size of 5 cm to 7 cm present in seawater were collected by hand using a low tide near a beach near Seogwang-ri west of Udo, Jeju Island (FIG. 1).

Example 2

Thickening Culture and Analysis of Bacterium-derived Bacteria

<2-1> Thickening and Culture Method of Red Nodules Derived Microorganisms

Flushing nodules collected in Example <1-2> were ground using Agate and Mortar. 5 g of the pulverized product of the red blood nodule was injected into a 500 ml Erlenmeyer flask bottle containing 100 ml of D-1 medium having the composition shown in Table 1 above, and the inlet of the Erlenmeyer flask was blocked with a sponge capable of introducing oxygen and carbon dioxide. After culturing for 7 days at room temperature and atmospheric pressure in sunlight, carbonate-producing microorganisms were enriched (FIG. 2). The D-1 medium used for the enrichment culture of the carbonate-producing microorganism was used after autoclaving at 121 ° C. for 20 minutes.

<2-2> Analysis of Thickening Culture of Carbonate Producing Microorganisms from Red Nodules

16S rRNA analysis using PCR after extracting nucleic acid from the enriched microorganism to confirm the diversity of the iron-reducing microorganism present in the enriched culture of the blush-derived carbonate-producing microorganisms enriched in Example <2-1> Was carried out. 9F (5'-GAG TTT GAT CCT GGC TCA G-3 '), 1542R (5'-AGA AAG GAG GTG ATC CAG CC-3') primer, known as universal primer, 20 μl of a reaction mixture of 0.1 μl of Taq polymerase (5 unit / μl, TAKARA), 2 μl of 10 × PCR buffer and 1.6 μl of dNTP was made to amplify a portion of the bacterial 16S rRNA by PCR. PCR amplification products were subjected to 1% agarose gel electrophoresis, stained with EtBr (ethidium bromide), and then PCR product generation was confirmed. The PCR product was amplified again using a primer with a GC clamp, and then subjected to Denaturing Gradient Gel Electrophoresis (DGGE). The band on the DGGE was extracted and subjected to sequencing.

As a result, Proteus Mira Billy's (Proteus mirabilis) and Marino tumefaciens Coral riyi (Marinobacterium coralli) the well, Vibrio find fun tee Syracuse (Vibrio alginolyticus), Vibrio Owen Shi (Vibrio owensii), Vibrio Cuiaba (Vibrio xuii), Vibrio Vibrio vulnificus , Vibrio fluvialis , Vibrio nereis , Vagococcus fluvialis , Fusobacterium perfoetens , Tyndalia A total of 234 microorganisms were identified, including Tindallia californiensis , Arcobacter marinus , Parabacteroides gordonii , and Prolixibacter bellariivorans . (Fig. 3).

Such microorganisms are expected to play a role in the production of red nodules. In other words, such a nodule is formed by a mechanism in which dissolved strontium in water is fixed by binding with a carbonate anion or hydrogen carbonate anion in water by the metabolism of such microorganisms, in particular carbonate-producing microorganisms, and a strontium carbonate mineral is formed. It seems to be. In addition, the formation process of the strontium carbonate mineral is determined that the various microorganisms as described above are fixed to the carbonate at a faster rate through a complex interaction.

Example 3

Fixation of Dissolved Strontium in Water and Synthesis of Strontium Carbonate Minerals

<3-1> Fixation of Dissolved Strontium in Water and Synthesis of Strontium Carbonate Minerals

Culture enriched in the Example <2-1> in a 500 ml Erlenmeyer flask bottle containing 100 ml of autoclaved D-1 medium having the composition shown in Table 1 (hereinafter, referred to as "enrichment culture"). Was inoculated at a concentration of 1 w / v% based on the volume of the medium, and 100 mmol of Sr-acetate was added to block the inlet of the Erlenmeyer flask with a sponge that can inject sunlight and carbon dioxide into the sunlight. The beach was reacted for 7 days at room temperature and laboratory pressure.

As a result, as the turbidity in the Erlenmeyer flask was increased after 4 days, white precipitates began to form, and a considerable amount of precipitate was observed after 7 days (Fig. 4).

<3-2> Geochemical and Mineralogical Characterization of the Formed Sediments

In order to analyze the geochemical and mineralogical characteristics of the precipitate produced in Example <3-1>, XRD and SEM-EDS analysis were performed. After the precipitate produced for the above analysis was separated using a centrifuge, the precipitate was dried at room temperature and then subjected to XRD, XRF and SEM-EDS on the dried precipitate.

As a result of XRD analysis of the resulting precipitate, the peak of d (104) was measured to be 3.52 mm 3 and found to be strotianite (FIG. 5). In addition, SEM-EDS analysis showed that the precipitate was spherical Strontianite (Fig. 6).

From the above results, it can be seen that the precipitate produced in Example <3-1> is fixed in the form of strontium carbonate mineral strontianite, using the enrichment culture of Example <2-1>. It was confirmed that the microorganisms derived from red nodules can fix the dissolved strontium in water with strontium carbonate minerals using the principle of metabolism.

In the above described exemplary embodiments of the present invention by way of example, the scope of the present invention is not limited only to the specific embodiments as described above, those skilled in the art to the scope described in the claims of the present invention It will be possible to change accordingly.

Claims (6)

1. Enriching the redness nodules-derived microorganisms; And

   Treating the enriched microorganisms with contaminated water and reacting under aerobic conditions;

   The dissolved strontium in the contaminated water is fixed with strontium carbonate mineral strontiumite (strontianite, SrCO ₃ ) is a method of fixing the dissolved strontium in contaminated water.
2. According to claim 1, wherein the step of enriching the microorganism

   Harvesting redness nodules; And

   And the step of culturing in an aerobic environment by putting the sampled flushing nodules into any one medium selected from the group consisting of calcium salt or magnesium salt, D-1 medium, MH medium and Saline medium. Method for fixing dissolved strontium in contaminated water, characterized in that.
3. The method of claim 2, wherein the calcium salt or magnesium salt is at least one selected from the group consisting of calcium acetate, magnesium acetate, and a mixture of calcium acetate and magnesium acetate. .
4. The method of claim 1, wherein the enriched microorganism is treated at a concentration of 0.5 w / v% to 1.5 w / v% based on the total volume of the contaminated water.
5. A composition for immobilizing dissolved strontium in contaminated water, which contains a concentrated culture of red blood nodule-derived microorganisms as an active ingredient, and fixes the dissolved strontium in contaminated water with strontium carbonate mineral strontianite (SrCO ₃ ).
6. The method of claim 5, wherein the flushing nodules concentrated cultures Vibrio know the derived microorganism fun tea kusu (Vibrio alginolyticus), Vibrio Owen Shi (Vibrio owensii), Vibrio Cui (Vibrio xuii), Vibrio block nipi kusu (Vibrio vulnificus), Vibrio fluvialis , Vibrio nereis , Proteus mirabilis , Marinobacterium coralli , Vagococcus fluvialis , Fusobacterium Fusobacterium perfoetens , Tindallia californiensis , Arcobacter marinus , Parabacteroides gordonii and Proxibacter bellaibivorans bellariivorans) to five characterized in that it comprises at least one of the microorganisms selected from the group consisting of Dissolved strontium be fixed composition.

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