CN104357035B - Biological bactericide for preventing and controlling SRB (sulfate reducing bacteria) in high-temperature water body and SRB inhibition method of bactericide - Google Patents

Biological bactericide for preventing and controlling SRB (sulfate reducing bacteria) in high-temperature water body and SRB inhibition method of bactericide Download PDF

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Publication number
CN104357035B
CN104357035B CN201410569095.9A CN201410569095A CN104357035B CN 104357035 B CN104357035 B CN 104357035B CN 201410569095 A CN201410569095 A CN 201410569095A CN 104357035 B CN104357035 B CN 104357035B
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srb
water body
jshd
temperature water
high temperature
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CN104357035A (en
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吴伟林
杨帆
姚峰
薛芸
孟章进
姜桂英
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China Petroleum and Chemical Corp
Sinopec Jiangsu Oilfield Co
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China Petroleum and Chemical Corp
Sinopec Jiangsu Oilfield Co
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    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
    • C09K8/54—Compositions for in situ inhibition of corrosion in boreholes or wells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
    • C09K8/52—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning
    • C09K8/528—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning inorganic depositions, e.g. sulfates or carbonates
    • E—FIXED CONSTRUCTIONS
    • E21—EARTH DRILLING; MINING
    • E21B—EARTH DRILLING, e.g. DEEP DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
    • E21B37/00—Methods or apparatus for cleaning boreholes or wells
    • E21B37/06—Methods or apparatus for cleaning boreholes or wells using chemical means for preventing, limiting or eliminating the deposition of paraffins or like substances
    • E—FIXED CONSTRUCTIONS
    • E21—EARTH DRILLING; MINING
    • E21B—EARTH DRILLING, e.g. DEEP DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
    • E21B41/00—Equipment or details not covered by groups E21B15/00 - E21B40/00
    • E21B41/02—Equipment or details not covered by groups E21B15/00 - E21B40/00 in situ inhibition of corrosion in boreholes or wells
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2208/00—Aspects relating to compositions of drilling or well treatment fluids
    • C09K2208/32—Anticorrosion additives

Abstract

The invention relates to a technology for inhibiting SRB (sulfate reducing bacteria) in oilfield produced liquid, in particular to a biological bactericide for preventing and controlling the SRB in a high-temperature water body and an SRB inhibition method of the bactericide. The biological bactericide is JSHD-4 geobacillustoebii, has a sequence as shown in a sequence table SEQ ID NO:1, is collected in China General Microbiological Culture Collection Center (CCMCC) on March 5, 2013, and has a Latin literature name of Geobacillus Toebii and a collection number of CGMCC NO 7273. The JSHD-4 geobacillustoebii is subjected to microbial aerobic fermentation to prepare industrial fermentation liquid with the bacterial solution concentration of 1.0*10＜6＞ to 1.3*10＜10＞ cfu/mL, the tolerable temperature is 50-95 DEG C, and the optimal growth temperature is 60-70 DEG C. After the multifunctional biological bactericide prepared from the JSHD-4 geobacillustoebii is thrown into a well, nitrate reducing bacteria (DNB) are quickly proliferated in the high-temperature environment in the well, so that the biological bactericide is suitable for directly treating the SRB in the high-temperature environment in the well on line.

Description

The bacteria agent of SRB and its method for suppressing SRB in preventing and treating high temperature water body
Technical field
The present invention relates to the sulfate reducting bacteria suppression technology field in the water body of oil field, more particularly to a kind of preventing and treating high-temperature water The bacteria agent of SRB and its method for suppressing SRB in body.
Background technology
Oil well etching problem long-standing problem oil exploration industry, is also the class that oil well production and scientific research personnel pay close attention to always Topic, is not preferably solved so far.Numerous studies show that oil well biological corrosion is mainly harmful microorganism sulfate reduction Bacterium（SRB）Grow in a large number, cause what sulfide concentration increase was caused.At present, oil well control SRB and sulfide are mainly taking Learn and physical method, there is high cost, easy secondary pollution, form the deficiencies such as drug resistance.
Oil field sulfate reductive bacterium Biological control collection is defeated gradually to be risen in recent years, and the technology mainly uses biological competition and washes in a pan The method eliminated, favorable factor is changed into by the replacement of microbial population by harmful microorganism problem；But the technology is mainly ground Study carefully the SRB that object is oilfield transportation system to administer, for this high temperature extreme environment of oil well（More than 50 DEG C）Research it is less.
Multifunctional bio microbial inoculum in the present invention can be used under 50～95 DEG C of High Temperatures In Oil-well environment so that nitrate The rapid hypertrophy diffusion of reduction flora (DNB), and when vivosphere and substrate is competed with SRB, DNB prioritizing selections are using oil field Substrate in system, the nutrient required for effectively preventing SRB from obtaining, so as to control the metabolic activity of SRB.
The content of the invention
The purpose of the present invention is the deficiency for SRB biological inhibition technologies in prior art, by oil field well high temperature Thermophilic multifunctional bio microbial inoculum is injected in environment, effectively suppresses the fast-growth of SRB in hot environment, what elimination had been produced Sulfide, while stimulating the growth of denitrifying bacteria, changes microorganisms in water structure of community, and so as to reach biological corrosion is prevented Purpose.
It is an object of the invention to provide in a kind of preventing and treating oil field high temperature water body environment SRB bacteria agent, the life Thing microbial inoculum is JSHD-4 ground bacillus, with sequence table SEQ ID NO：Sequence shown in 1, is preserved in China Microbiological bacterium Plant preservation management commission person and understand common micro-organismss collection, Latin name is Geobacillustoebii, deposit number It it is on March 5th, 2013 for the preservation dates of CGMCC NO 7273.
In the present invention, it is 1.0 × 10 that the JSHD-4 ground bacillus are obtained bacterial concentration by microorganism aerobic fermentation6 ～1.3 × 1010The industrial fermentation liquid of cfu/mL, tolerable temperature is 50-95 DEG C, and optimum growth temp is 60-70 DEG C.The present invention In JSHD-4 ground bacillus tolerable temperature it is high, be adapted to the suppression of SRB in the subsurface environment of high temperature, prevent down-hole from giving birth to Thing corrodes.
It is a further object of the invention to provide a kind of suppressed in the high temperature water body environment of oil field using above-mentioned bacteria agent The method of SRB, in the amount of oil field high temperature water body the multifunctional bio microbial inoculum of following ratio is added：JSHD-4 ground bacillus：It is logical It is 1.0 × 10 to cross microorganism aerobic fermentation and bacterial concentration is obtained6～1.3 × 1010The industrial fermentation liquid of cfu/mL, the industry is sent out Zymotic fluid adds ratio for oil field high temperature water body volume 0.5～1%；The mg/L of sodium nitrate 20～30；The mg/ of sodium nitrite 50～150 L；The mg/L of sodium dihydrogen phosphate 5～10
As the further improvement of the inventive method, the industrial fermentation that the JSHD-4 ground bacillus pass through aerobic fermentation The concentration of liquid is 1.0～1.3 × 108Cfu/mL, adds ratio for oil field high temperature water body volume 0.8%；The mg/L of sodium nitrate 25；It is sub- The mg/L of sodium nitrate 75；The mg/L of sodium dihydrogen phosphate 8.
Improve as another step of the present invention, in the method for the present invention, in target oil well the multifunctional bio is added During microbial inoculum, first well casing gas are pressed to casing pressure back to zero, JSHD-4 ground bacillus bacterium solution is directly added using impact type and added Enter in oil well casing, sodium nitrate, sodium nitrite and sodium dihydrogen phosphate are first dissolved in the water of 25-30L after weighing in proportion, so Added in addition oil well casing using impact type afterwards, 50L water is finally added into oil well casing again.
The invention has the beneficial effects as follows：The JSHD-4 ground bacillus of the present invention can be in 50～95 DEG C of High Temperatures In Oil-well ring Use under border, the fermentation of strain Jing biology aerobics can obtain bacterial concentration for 1.0 × 106～1.3 × 1010The industry of cfu/mL spore Fermentation liquid, using behind the multifunctional bio microbial inoculum input down-hole that JSHD-4 ground bacillus are prepared, makes under the hot environment of down-hole The rapid hypertrophy diffusion of nitrate reduction flora (DNB) is obtained, can effectively suppress the fast-growth of SRB in hot environment, eliminated The sulfide for having produced, while the growth of the microorganism denitrifying bacteria that stimulates emulation, changes microorganisms in water structure of community, its Sulfide suppression ratio effectively extends the service life of pipeline more than 93%, improves water quality, substantially reduces production cost.
Biological deposits
Microorganism involved in the present invention has been filed on biological deposits：
Specific embodiment
Embodiment 1
The JSHD-4 ground bacillus of the present invention are screened by following process：
（1）Sample collecting
The production fluid of 7 mouthfuls of oil wells is gathered in Jiangsu oilfield, sample ID is respectively：CH2-43、CH2-42、CH2-36、 T83-1、T83-10、T83-7、H88-10。
(2) culture medium configuration
Enrichment medium is made up of solution A and B solution mixing, wherein：
Solution A：The g of inorganic salt K1 2.0, the g of agedoite 1.0, the ml of distilled water 500, pH value 7.0 ~ 7.2；
B solution：The g of sodium citrate 8.5, the g of sodium acetate 2, glucose 2 g, KH2PO40.5 g, K2HPO40.5 g, MgSO4·7H2O 1.0 g, FeCl3·6H2O 0.05 g, CaCl2The g of 0.1 g, yeast extract 0.5, distilled water 500 ml.PH value 7.0 ~ 7.2.
NRB fluid mediums：A liquid and B liquid 20 min of independent 121 DEG C of sterilizings, equal proportion mixing after sterilizing.
NRB solid mediums：It is each in A liquid and B liquid to add the agar that mass ratio is 1.5% ~ 2%, individually wait ratio after sterilizing Example mixing.
（3）Enrichment culture
By step（1）In the mixing of 7 mouthfuls of produced liquid in oil well sample equal proportions, be subsequently adding NRB fluid mediums, be respectively placed in 65 ℃、75 ℃（Whether 70 DEG C should be changed to）Aerobic culture in incubator；Meanwhile, done with the same Produced Liquid sterilizing sample for processing For control sample.Cultivate to be seeded in identical NRB fluid medium with 5% inoculum concentration after 6d under the conditions of 65 DEG C and carry out switching training Support, transfer again after 3 d of culture, switching culture 3 times carries out domestication culture under the conditions of being then forwarded to 70 DEG C.Determine after culture K1 ion concentrations are shown in Table 1 in culture fluid.
Through three switchings, 65 DEG C, culture solution is muddy under the conditions of 70 DEG C, and control sample culture fluid is clarified, and shows reality Microorganism raised growth in tissue culture nutrient solution is tested, meanwhile, shown by the culture fluid K1 concentration mensurations of table 1,70 DEG C of domestications culture 2 d, K1 Concentration is reduced to 0.24 g/L by 2.0 g/L, and 65 DEG C of third time switching culture fluid K1 concentration mensurations are 0, solution K1 after culture Ion concentration is greatly lowered explanation growth of microorganism and consumes in a large number with K1 ions simultaneously, shows that experimental group culture fluid contains nitre Hydrochlorate reducing bacteria, and grow under experimental conditions vigorous.
（4）Strains separation purification
The samples of 65 DEG C of three switchings, 70 DEG C of domestications are applied to into NRB solid medium flat board cultures, from gained culture Picking single bacterium colony is cultivated in conical flask on flat board, and after 3 d of culture 4 DEG C of Refrigerator stores are placed in.
（5）Nitrate reduction bacterium DNA extraction and identification
（I）DNA extraction
Nitrate reduction bacterium enrichment culture after isolating and purifying 3 days, with 2 mL centrifuge tubes under the conditions of 12000 × g Cell is collected by centrifugation, nitrate reduction bacterium DNA is extracted using Axygen DNA extraction kits, concrete operation step is as follows：
1) 450 μ L lysates, vortex vibration 15 s, 65 DEG C of min of water-bath 10 are added in the centrifuge tube equipped with cell；
2) 400 μ L protein denaturants and 1 mL phase separation liquid (4 DEG C of pre-coolings) are continuously added, is firmly mixed, 12000 × g is centrifuged 2 min；
3) phase is abandoned, retains INTERPHASE CARBIDE PRECIPITATION and lower phase, add the phase separation liquid of 4 DEG C of pre-coolings of 1mL, mixing, 12000 × g 2 min are centrifuged；
4) phase is abandoned, (filter is placed in 2 mL centrifuge tubes by lower phase transfer to filter）, 12000 × g, 1 min of centrifugation；
5) filter is abandoned, adds 400 μ L DNA to combine liquid, mix homogeneously in filtrate；
6) pipe will be prepared to be placed in 2 mL centrifuge tubes, the mixed liquor immigration in step (5) is prepared in pipe, 12000 × g 1 min is centrifuged；
7) abandon filtrate, pipe will be prepared and put back in former 2 mL centrifuge tubes, add 500 μ L flushing liquor W1,12000 × g from The min of the heart 1；
8) abandon filtrate, pipe will be prepared and put back in former 2 mL centrifuge tubes, add 700 μ L flushing liquor W2,12000 × g from The min of the heart 1；
9) washed once with 700 μ L flushing liquor W2 again in the same way；
10) filtrate is abandoned, pipe will be prepared and put back in former 2 mL centrifuge tubes, 12000 × g is centrifuged 1 min；
11) pipe will be prepared to be placed in 1.5 mL centrifuge tubes of another cleaning, in silica films central authorities plus 100-200 μ L Eluent or deionized water, are stored at room temperature 1 min, and 12000 × g is centrifuged 1 min eluted dnas, be stored at -20 DEG C in case With.
（II）Sample P CR is expanded
By the DNA of the JSHD04 for extracting, performing PCR amplification is entered using 16S rDNA gene universal primer 1492R and 27F.
1) bacterial 16 S rDNA PCR reaction systems
PCR reaction systems composition is shown in Table 2.
2) primer sequence
2) primer sequence
1492R：5‘-GGTTACCTTGTTACGACTT-3’
27F：5 ' ＞ CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGAGAGTTTGATCTTGGC TCAG ＜ 3 '
3) PCR response procedures
1.95 DEG C of 3 min
2.94 DEG C of 40 sec
3.52 DEG C of 45 sec
The times of 4.Goto 2 35
5.72 DEG C of 10 min
（III）Sequencing result
65 DEG C of enriched samples of Jiangsu oilfield production fluid isolate bacterium colony（Numbering JSHD-4）, picking single bacterium colony enrichment culture, Cultured cells extracts DNA, and pcr amplification product is sequenced, if as a result such as SEQ ID NO：Sequence shown in 1.
Jing analyse and compare with Jing analyse and compare withG. toebii strain R-32639Similarity is 97%, is ground spore One subspecies of bacillus.
Embodiment 2
Inhibitions of the JSHD-4 to SRB under the conditions of System of Laboratories
SRB culture medium prescriptions are improved according to table 3, by solvent of sterilized water culture medium configuration, 121 DEG C of culture medium Jing are carried out Sterilize 20 min, and every liter of culture medium is separately added into again trace element solution after cooling（Composition is as shown in table 4）1 mL, vitamin are molten Liquid（Composition is as shown in table 5）The Ferrous ammonium sulfate 0.1g and the g of L-cysteine hydrochloride 0.5 of 1 mL and Jing ultraviolet disinfections；From The SRB strains preserved with inoculating loop picking on solid slope（Desulfotomaculum）3 ~ 4 rings are inoculated in SRB culture medium, at 65 DEG C Lower culture 48 hours, obtains the SRB bacterium solutions of fresh enrichment culture.
Simultaneously the JSHD-4 for preserving is used into NRB fluid mediums at 75 DEG C（With the NRB liquid cultures in example 1 Base）The d of activation culture 1, obtains the JSHD-4 bacterium solutions of fresh enrichment culture, then by 2% inoculative proportion by JSHD-4 bacterium solutions and SRB bacterium solutions are inoculated in the culture medium of improvement SRB（Assay flask is the anaerobism bottle of 250ml）, and add the sodium nitrate of 10 mmol/L Cultivated at 65 DEG C, while not being inoculated with nitrate reduction to be only inoculated with the sodium nitrate of 10 mmol/L of SRB bacterium solutions and addition Bacterium for matched group, compare culture fluid change, by detecting H2The content of S to characterize multifunctional bio microbial inoculum in JSHD-4 pair The rejection ability of SRB activity, testing result is as shown in table 6）.After cultivating 7 d, only add the nitric acid of 10 mmol/L of SRB and addition H in the matched group of sodium2S contents are very high, and add containing JSHD-4 inhibition system in H2S concentration is equal in the 3rd d and the 7th d Keep very low, the activity for showing SRB in experimental group is substantially suppressed.
Embodiment 3
First by denitrifying bacteria JSHD-4 by conventional microbiological aerobic fermentation prepare brood cell's concentration for 1.0 × 106Cfu/mL industrial fermentation liquid, the industrial fermentation liquid can be stored using 20L Plastic Drums, then take sodium nitrate, sodium nitrite and phosphorus Acid dihydride sodium is commercially available technical grade product, mixes in proportion when using, it is also possible to sequentially add target oil well produced liquid.
JSHD-4 multifunctional bios microbial inoculum of the present invention is put into into Jiangsu oilfield Wang39Jing in following ratio, the before processing well is produced The sulfide concentration for going out liquid is 28mg/L.Dosage is：JSHD-4 industrial fermentation liquid（1.0×106cfu/mL）, dosage is to adopt Go out the 0.5% of liquid hold-up；Sodium nitrate：20mg/L；Sodium nitrite：50mg/L；Sodium dihydrogen phosphate：5mg/L
When adding above-mentioned multifunctional bio microbial inoculum in target oil well, first well casing gas are pressed to casing pressure back to zero, JSHD-4 industrial fermentations liquid is directly added in addition oil well casing using impact type, sodium nitrate, sodium nitrite and sodium dihydrogen phosphate First it is dissolved in the water of 25L after weighing in proportion, is then added in addition oil well casing, finally again to oil well set using impact type 50L water is added in pipe.Sulfide content is irregularly detected simultaneously, and supplements addition multifunctional bio microbial inoculum in good time；To keep suppression Effect processed.Inhibition sulfide change curve as shown in Figure 1,60d suppression ratio is up to 93.14%.
Embodiment 4
Difference from Example 3 is, enforcement oil well is Jiangsu oilfield Wei2-53Jing, sulfide 128.4mg/L, JSHD-4 industrial fermentation liquid spore concentration is 1.3 × 1010Cfu/mL, dosage is the 1% of oil well produced liquid hold-up；Sodium nitrate： 30mg/L；Sodium nitrite：150mg/L；Sodium dihydrogen phosphate：10mg/L.Inhibition sulfide change curve as shown in Figure 1, 60d suppression ratio is up to 97.73%.
Embodiment 5
Difference from Example 3 is, enforcement oil well is Jiangsu oilfield Huang 88-39 wells, sulfide 56mg/L, JSHD-4 Industrial fermentation liquid concentration is 1.3 × 108Cfu/mL, dosage is produce liquid hold-up 0.8%；Sodium nitrate：25mg/L；Nitrous acid Sodium：75mg/L；Sodium dihydrogen phosphate：8mg/L.Inhibition sulfide change curve as shown in Figure 1,60d suppression ratio reaches 94.93%。
Sequence table
<110>Sinopec Group；Sinopec Co., Ltd. Jiangsu Oil Field Branch
<120>The bacteria agent of SRB and its method for suppressing SRB in preventing and treating high temperature water body
<160>1
<210>1
<211>1012
<212>DNA
<213>Artificial sequence
<400>1
ATCATGCAAG TCGAGCGGAC CGAACGGAAG CTTGCTTCTG TTCGGTTAGC GGCGGACGGG 60
TGAGTAACAC GTGGGTAACC TGCCCGTAAG ACCGGGATAA CTCCGGGAAA CCGGGGCTAA 120
TACCGGATAA CACCGAAGAC CGCATGGTCT TTGGTTGAAA GGTGGCTTTT GCTACCACTT 180
ACGGATGGGC CCGCGGCGCA TTAGCTAGTT GGTGAGGTAA CGGCTCACCA AGGCGACGAT 240
GCGTAGCCGG CCTGAGAGGG TGACCGGCCA CACTGGGACT GAGACACGGC CCAGACTCCT 300
ACGGGAGGCA GCAGTAGGGA ATCTTCCGCA ATGGACGAAA GTCTGACGGA GCGACGCCGC 360
GTGAGCGAAG AAGGTCTTCG GATCGTAAAG CTCTGTTGTT AGGGAAGAAG AAGTACCGTT 420
CGAATAGGGC GGTACGGTGA CGGTACCTAA CGAGAAAGCC CCGGCTAACT ACGTGCCAGC 480
AGCCGCGGTA ATACGTAGGG GGCGAGCGTT GTCCGGAATT ATTGGGCGTA AAGCGCGCGC 540
AGGCGGTCC CTTAAGTCTGA TGTGAAAGCC CACGGCTCAA CCGTGGAGGG TCATTGGAAA 600
CTGGGGGACT TGAGTGCAGA AGAGGAGAGC GGAATTCCAC GTGTAGCGGT GAAATGCGTA 660
GAGATGTGGA GGAACACCAG TGGCGAAGGC GGCTCTCTGG TCTGTAACTG ACGCTGAGGC 720
GCGAAAGCGT GGGGGAGCAA ACAGGATTAG ATACCCTGGT AGTCCACGCC GTAAACGATG 780
AGTGCTAAGT GTTAGAGGGG TTTTCCCTTT AGTGCTGTAG CTAACGCGTT AAGCACTCCG 840
CCTGGGGAGT ACGGCGCATG CTGAACTCAA GAATTGACGG GGCCCGCACA AGCGTGAGCA 900
TGTGGTTTAA TTCGAGCACG CGAGACCTTA CAGTCTGACA TCCCTGACAC CTGGAAATGC 960
GTTCCCCCTC GGGGGACAGG TGACAGGGGG GCATGGTGTC GTCACACTCG TG 1012
<210>2
<211>19
<212>DNA
<213>Artificial sequence
<400>2
GGTTACCTTGTTACGACTT
<210>3
<211>60
<212>DNA
<213>Artificial sequence
<400>3
CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGAGAGTTTGATCTTGGCTCAG

Claims (5)

1. it is a kind of preventing and treating high temperature water body in SRB bacteria agent, it is characterised in that the bacteria agent be JSHD-4 ground spore bar Bacterium, with sequence table SEQ ID NO：Sequence shown in 1, being preserved in Chinese microorganism strain preservation management commission person can be common Organism Depositary, Latin name is Geobacillustoebii, deposit number is the preservation days of CGMCC NO 7273 Phase is on March 5th, 2013.
2. in the preventing and treating high temperature water body described in claim 1 SRB bacteria agent, it is characterised in that JSHD-4 ground spore It is 1.0 × 10 that bacillus is obtained bacterial concentration by microorganism aerobic fermentation6～1.3 × 1010The industrial fermentation liquid of cfu/mL, tolerance Temperature is 50-95 DEG C, and optimum growth temp is 60-70 DEG C.
3. the method that the bacteria agent described in a kind of claim 2 suppresses SRB in high temperature water body, it is characterised in that high by oil field The amount of warm water body adds the multifunctional bio microbial inoculum of following ratio：The industry that the JSHD-4 ground bacillus pass through aerobic fermentation Fermentation liquid adds ratio for oil field high temperature water body volume 0.5～1%；The mg/L of sodium nitrate 20～30；Sodium nitrite 50～150 mg/L；The mg/L of sodium dihydrogen phosphate 5～10.
4. the method that bacteria agent according to claim 3 suppresses SRB in high temperature water body, it is characterised in that high by oil field The amount of warm water body adds the multifunctional bio microbial inoculum of following ratio：The industry that the JSHD-4 ground bacillus pass through aerobic fermentation The concentration of fermentation liquid is 1.0 × 108～1.3 × 108Cfu/mL, adds ratio for oil field high temperature water body volume 0.8%；Sodium nitrate 25 mg/L；The mg/L of sodium nitrite 75；The mg/L of sodium dihydrogen phosphate 8.
5. the method that the bacteria agent according to claim 3 or 4 suppresses SRB in high temperature water body, it is characterised in that to target When the multifunctional bio microbial inoculum is added in oil well, first well casing gas are pressed to casing pressure back to zero, JSHD-4 ground bacillus Bacterium solution is directly added in addition oil well casing, after sodium nitrate, sodium nitrite and sodium dihydrogen phosphate are weighed in proportion using impact type First it is dissolved in the water of 25-30L, is then added in addition oil well casing using impact type, finally adds into oil well casing again 50L water.
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