CN106987538A - One plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 and its application - Google Patents
One plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 and its application Download PDFInfo
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- CN106987538A CN106987538A CN201710260893.7A CN201710260893A CN106987538A CN 106987538 A CN106987538 A CN 106987538A CN 201710260893 A CN201710260893 A CN 201710260893A CN 106987538 A CN106987538 A CN 106987538A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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Abstract
The invention discloses one plant of black and odorous water ammonia nitrogen degradation bacterium AW0H4 and its application, China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved in, preservation date is on January 10th, 2017, and deposit number is CGMCC No.13544.The bacterial strain（Pseudomonas sp. AW0H4）Isolated from black smelly water, bacterium colony is in faint yellow.NH in the black smelly water of Tianjin swag3N, COD, TP are respectively 52.10 mg/L, 91.83 mg/L, 11.75 mg/L, belong to the black smelly water of severe.Experimental result shows that 10 d ammonia nitrogen degradations rates are 90.67%, and ammonia nitrogen concentration is less than the mg/L of black and odorous water limit value 8；Certain degradation is respectively provided with to indexs such as COD and total phosphorus simultaneously.Therefore the bacterial strain possesses tremendous potential in the biological prosthetic engineering of black and odorous water, has a extensive future.
Description
Technical field
The invention belongs to microbial technology field, it is related to one plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 and its application.
Background technology
In the last few years, city river had had a strong impact on the normal economic society of people with open water black and odorous water problem and given birth to
It is living.A series of its ecological environment problem triggered, can not only constitute a threat to, also badly damaged city to health, living environment
City's image.2015, State Council's issue《Water prevention and cure of pollution action plan》（Referred to as " ten, water "）Black and odorous water problem is carried
Go out to be distinctly claimed, to the year two thousand twenty, China's region and above completed region of the city black and odorous water are controlled within 10%；To the year two thousand thirty,
Completed region of the city black and odorous water is totally eliminated.As can be seen here, solve and the improvement black smelly problem of urban water-body is extremely urgent.
Up to now, the Forming Mechanism of black and odorous water and features of pollution are tentatively clear and definite, but Treatment process is still constantly being sent out
During exhibition updates.Black and odorous water denitrogenates method, main using physics, chemistry and biological method both at home and abroad in the last few years.Applying thing
Reason and chemical method solve generally to cure the symptoms, not the disease in the practice of black and odorous water denitrogenation, and costly, while having triggered it
Its ecological environment problem, it is impossible to realize long-term management goal.
Microbial denitrogenation method can fundamentally remove ammonia nitrogen in black and odorous water, be external Innovation Input in recent years most
Greatly, technology with fastest developing speed.Microbial treatment method core is to research and develop Black Liquor with Efficient Bacteria, by the metabolic activity of microorganism, to water
The ammonia nitrogen of various forms in body is shifted, converted and degraded, so as to improve water quality of river.Because foreign countries control in black and odorous water
Search time in terms of reason is more early, and China relatively lags behind in Related Research Domain, so needing to strengthen high-efficiency ammonia in black and odorous water
The screening and research work of nitrogen degradation bacterium.
The content of the invention
The purpose of invention is to provide one plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 and its application.
In order to realize the object of the invention, one plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 is to be separated by inventor from black smelly water
Out, the identified bacterial strain belongs to pseudomonas, is named asPseudomonas sp. AW0H4, on January 10th, 2017
In China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation, deposit number is CGMCC No.13544.
Bacterial strainPseudomonas sp. AW0H4Morphological feature to include it be shaft-like, no gemma, thalline size is
（0.5-0.6）μ m (1.5-1.7) μm, bacterium colony is into circle, and neat in edge is smooth, in faint yellow on LB culture mediums.It is leather
Lan Shi feminine gender aerobic bacterias, its molecular biology identification result is：16S is carried out using gene order being sequenced by ncbi database
RDNA sequences are contrasted, with Pseudomonas alba affiliation recently, up to 99%.
To bacterial strain of the present inventionPseudomonas sp. AW0H4Ammonia nitrogen degradation experiment is carried out, the bacterial strain is pressed into volume fraction
In the 2% access black smelly water of certain severe, shaking table culture is put into, NH in black smelly water is determined daily3- N, COD, TP concentration value.Experimental result
It has been shown that, 10 d ammonia nitrogen concentrations be less than the mg/L of black and odorous water limit value 8, black smelly water water quality and outward appearance be improved significantly, while bacterium
Strain is also respectively provided with certain degradation to the index such as COD and total phosphorus in black smelly water.
Present invention has the advantages that sift outPseudomonas sp. AW0H4Bacterium, which possesses, effectively removes severe black and odorous water
The ability of middle ammonia nitrogen, a kind of environmental protection, safe long-acting method are provided for the biological prosthetic engineering of black and odorous water.
Brief description of the drawings
Fig. 1 is bacteriumPseudomonas sp. AW0H4To ammonia nitrogen nutrient solution ammonia nitrogen in solution degradation effect figure.
Fig. 2 is bacteriumPseudomonas sp. AW0H4Ammonia nitrogen degradation design sketch in smelly water black to severe.
Embodiment
Embodiment 1
One plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 separation and identification：
The black smelly water ammonia nitrogen degradation bacterium AW0H4 of one plant of present embodiment is preserved in Chinese microorganism strain on January 10th, 2017
Preservation administration committee common micro-organisms center, deposit number is CGMCC No.13544, and the bacterial strain belongs to pseudomonas,
It is named asPseudomonas sp. AW0H4。
One plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 in present embodiment, it is obtained using conventional separation, purifying, screening technique
, specific method is as follows：
Take the black smelly L of water 2 of Tianjin swag, stand 1 h, take at the middle and upper levels black smelly water be used to test, by volume fraction be 5% add to
In sterilized LB culture mediums, 24 h are cultivated in 28 DEG C, take 5 mL nutrient solutions to be inoculated in new enriched medium, so repeatedly
5 generations.The nutrient solution after 5 generations of enrichment is taken, according to 10-3-10-8Different proportion dilutes, and 100 μ L difference gradient bacterium solutions are coated on ammonia nitrogen
On isolation medium, picking single bacterium colony cultivates 24 h in 28 DEG C, observes the growing state of bacterium, and selection growth is fast, bacterium colony is big, number
The many bacterium colonies of amount are purified using trilinear method, and single colony frozen preservation after purification is standby.
Culture medium used includes LB culture mediums in strain isolation purge process：The g of tryptone 10.00, yeast extract
The g of 5.00 g, NaCl 10.00.Isolation medium：The g of glucose 5.00,（NH4）2SO4 0.50 g,NaCl 2.00 g,
FeSO4·7H2O 0.40 g, K2HPO41.00 g, MgSO4·7H2The g of O 0.50, distilled water 1 L, pH 7.2-7.4.
Obtained strains are carried out with molecular biology identification, strain idenfication technology path is protected including extracting genome DNA 16S bacterium
Sequence PCR amplifications → 3730 are kept to be sequenced.Extracting genome DNA mainly uses liquid nitrogen method smudge cells, and SDS-CTAB methods extract bacterium
Plant genome.Primer employed in 16S bacterium conserved sequence PCR amplification steps is 27f：AGAGTTTGATCMTGGCTCAG,
1492R:TACGGYTACCTTGTTACGACTT；Reaction system：H2O 17.8 uL, Buffer 3 uL, dNTP 2 ul,
Primer1 3 uL, Primer2 3 uL, the uL of DNA profiling 1, the ul of enzyme 0.2, the uL of cumulative volume 30；Reaction condition：95 DEG C of pre- changes
Property 5 min, 95 DEG C of 35cycle denaturation 30 sec, 55 DEG C of annealing 30sec, 72 DEG C of extension 1 min, 72 DEG C of extension 10min, 12
DEG C forever insulation.After the identification of electrophoresis PCR primer, upper machine sequencing.By ncbi database by the 16S rDNA of this bacterial strain
Gene order carries out Blast comparisons, and comparison result is shown, this bacteriumPseudomonas sp. AW0H4WithPseudomonas sp. CJ-S-R2A3Affiliation is nearest, up to 99%.
Embodiment 2
Degradeds of one plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 to ammonia nitrogen nutrient solution：
Prepare ammonia nitrogen nutrient solution:The g of glucose 5.00,（NH4）2SO40.40 g, NaCl 2.00 g, FeSO4·7H2O 0.40
G, K2HPO41.00 g, MgSO4·7H2The g of O 0.50, distilled water 1.0 L, pH 7.2-7.4.In an aseptic environment, according to 1%
Volume fraction accessPseudomonas sp. AW0H4Bacterium solution, it is 28 DEG C, 120 r/min to set incubator temperature.Exist respectively
0 h, 6 h, 12 h, 24 h, 48 h, 72 h determine ammonia nitrogen concentration.Experimental result shows that initial ammonia nitrogen concentration is 84.85 mg/
L, 48 h ammonia nitrogen degradation rates are up to 85%.Ammonia nitrogen concentration is shown in 15 more than mg/L according to experiment effect in severe black and odorous water,
The bacterial strain possesses the ability of ammonia nitrogen in the quick processing black smelly water of severe while can also have certain ability using COD and phosphorus.
Embodiment 3
Degradeds of one plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 to ammonia nitrogen in black smelly raw water：
In the black smelly water of Tianjin swag, NH3- N, COD, TP are respectively 52.10 mg/L, 91.83 mg/L, 11.75 mg/L, category
In severe black and odorous water.Take this black smelly L of water 1, stand 1h, take at the middle and upper levels that the black mL of smelly water 100 is in 500 mL shaking flasks, to shaking flask
2 mL bacterium solutions of middle access, it is 28 DEG C, 120 r/min to set incubator temperature.Respectively in 0h, 0.5 d, 1d, 2 d, 3 d, 4 d, 5
D, 6 d, 7 d, 8 d, 9 d, 10 d determine ammonia nitrogen concentration value.Experimental result shows that 10 d ammonia nitrogen degradations rates are 90.67%, ammonia nitrogen
Less than the mg/L of black and odorous water limit value 8, its degraded to indexs such as COD, TP in black smelly water also has certain effect to concentration in addition.
Nucleotides sequence list
<110>Austria is（Tianjin）Environmental Protection Technology Co., Ltd
Rich member restoration of the ecosystem（Beijing）Co., Ltd
Nankai University
<120>One plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 and its application
<130> 2017
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1215
<212> DNA
<213>Pseudomonas alba（Pseudomonas sp.）
<400> 1
ggcatggcgc agctacacat gcagtcgagc ggatgagagg agcttgctcc tggattcagc 60
ggcggacggg tgagtaatgc ctaggaatct gcctggtagt gggggacaac gtttcgaaag 120
gaacgctaat accgcatacg tcctacggga gaaagcaggg gaccttcggg ccttgcgcta 180
tcagatgagc ctaggtcgga ttagctagtt ggtgaggtaa tggctcacca aggcgacgat 240
ccgtaactgg tctgagagga tgatcagtca cactggaact gagacacggt ccagactcct 300
acgggaggca gcagtgggga atattggaca atgggcgaaa gcctgatcca gccatgccgc 360
gtgtgtgaag aaggtcttcg gattgtaaag cactttaagt tgggaggaag ggttgtagat 420
taatactctg cgattttgac gttaccgaca gaataagcac cggctaactc tgtgccagca 480
gccgcggtaa tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta 540
ggtggtttgt taagttggat gtgaaatccc cgggctcaac ctgggaactg catccaaaac 600
tggcaagcta gagtatggta gagggtggtg gaatttcctg tgtagcggtg aaatgcgtag 660
atataggaag gaacaccagt ggcgaaggcg accacctgga ctgatactga cactgaggtg 720
cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgtc 780
aactagccgt tgggagcctt gagctcttag tggcgcagct aacgcattaa gttgaccgcc 840
tggggagtac ggccgcaagg ttaaaactca aatgaattga cggggcccgc acaagcggtg 900
gagcatgtgg attaattcga agcacgcgag aaccttacca gccttgacat ccatgaactt 960
tcagagatga ttgtgcttcg ggaacattga aacagtgctg catgctgtcg tcagctcgtg 1020
tctgagatgt gggtagtccc gtacgagcgc acctggtcta gtacagcacg ttatgtggcc 1080
ctctaggaac tgcgtgacac gagaaggtgg gatgacgtca gtcatcatgc tacggctgac 1140
taacacggct aatggtcggt ccagggtgca gccgagtgac tatcaacgat cgaatcgatc 1200
ggactggacc gaacg 1215
Claims (3)
1. one plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 and its application, it is characterised in that：It is on January 10th, 2017 is preserved in
State's Microbiological Culture Collection administration committee common micro-organisms center, deposit number is CGMCC No.13544, and the bacterial strain belongs to
Pseudomonas（Pseudomonas sp.）, it is named asPseudomonas sp. AW0H4。
2. one plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 according to claim 1 and its zymotic fluid/or its metabolite are black
Application in smelly water body ammonia nitrogen degradation.
3. one plant of black smelly water ammonia nitrogen degradation bacterium AW0H4 according to claim 1, it is characterised in that：The strain isolation culture
Base is the g of glucose 5.00,（NH4）2SO4 0.50 g,NaCl 2.00 g,FeSO4·7H2O 0.40 g, K2HPO41.00 g,
MgSO4·7H2The g of O 0.50, distilled water 1 L, pH 7.2-7.4；Cultivation temperature is 28-35 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109402029A (en) * | 2018-12-20 | 2019-03-01 | 北京本农科技发展有限公司 | Isolation and purification method, ammonia nitrogen degradation strain and the application of ammonia nitrogen degradation bacterium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090109246A (en) * | 2008-04-15 | 2009-10-20 | 대한민국(관리부서:농촌진흥청) | Diminish of offensive odor by pseudomonas stutzeri nist-1 |
| CN102776145A (en) * | 2012-07-31 | 2012-11-14 | 山东大学 | Denitrifying polyphosphate accumulation bacterium and application of same in sewage treatment |
| CN103215202A (en) * | 2013-03-20 | 2013-07-24 | 南京大学 | Aerobic denitrifying strain and its application |
-
2017
- 2017-04-20 CN CN201710260893.7A patent/CN106987538A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090109246A (en) * | 2008-04-15 | 2009-10-20 | 대한민국(관리부서:농촌진흥청) | Diminish of offensive odor by pseudomonas stutzeri nist-1 |
| CN102776145A (en) * | 2012-07-31 | 2012-11-14 | 山东大学 | Denitrifying polyphosphate accumulation bacterium and application of same in sewage treatment |
| CN103215202A (en) * | 2013-03-20 | 2013-07-24 | 南京大学 | Aerobic denitrifying strain and its application |
| CN103215202B (en) * | 2013-03-20 | 2014-07-23 | 南京大学 | Aerobic denitrifying strain and its application |
Non-Patent Citations (2)
| Title |
|---|
| CHUNE LI，等: "Removal of nitrogen by heterotrophic nitrification–aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24", 《BIORESOURCE TECHNOLOGY》 * |
| 张凯，等: "好氧反硝化细菌ＷＩＴ－１的分离鉴定及其脱氨氮特性", 《武汉工程大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109402029A (en) * | 2018-12-20 | 2019-03-01 | 北京本农科技发展有限公司 | Isolation and purification method, ammonia nitrogen degradation strain and the application of ammonia nitrogen degradation bacterium |
| CN109402029B (en) * | 2018-12-20 | 2020-11-10 | 北京本农科技发展有限公司 | Separation and purification method of ammonia nitrogen degrading bacteria, ammonia nitrogen degrading bacteria and application |
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