WO2013111065A8 - Three-dimensional scaffold consisting of fibroin and polyethylene glycol useful for cell cultures - Google Patents

Three-dimensional scaffold consisting of fibroin and polyethylene glycol useful for cell cultures Download PDF

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Publication number
WO2013111065A8
WO2013111065A8 PCT/IB2013/050577 IB2013050577W WO2013111065A8 WO 2013111065 A8 WO2013111065 A8 WO 2013111065A8 IB 2013050577 W IB2013050577 W IB 2013050577W WO 2013111065 A8 WO2013111065 A8 WO 2013111065A8
Authority
WO
WIPO (PCT)
Prior art keywords
fibroin
polyethylene glycol
cell cultures
dimensional scaffold
scaffold consisting
Prior art date
Application number
PCT/IB2013/050577
Other languages
French (fr)
Other versions
WO2013111065A1 (en
Inventor
Francesco Cilurzo
Paola Minghetti
Original Assignee
Pharmafilm S.R.L.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmafilm S.R.L. filed Critical Pharmafilm S.R.L.
Publication of WO2013111065A1 publication Critical patent/WO2013111065A1/en
Publication of WO2013111065A8 publication Critical patent/WO2013111065A8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/80—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special chemical form
    • A61L2300/802—Additives, excipients, e.g. cyclodextrins, fatty acids, surfactants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins

Abstract

Disclosed are spongy structures with interconnected pores consisting of fibroin from Bombyx mori and polyethylene glycol with a molecular weight of less than 1500, in the fibroin/polyethylene glycol ratio of between 99:1 and 90:10 parts, obtained by regenerating fibroin, mixing with polyethylene glycols, freeze-drying in aqueous solution in the absence of organic solvents, and sterilising under saturated steam.
PCT/IB2013/050577 2012-01-24 2013-01-23 Three-dimensional scaffold consisting of fibroin and polyethylene useful for cell cultures WO2013111065A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITMI2012A000076 2012-01-24
IT000076A ITMI20120076A1 (en) 2012-01-24 2012-01-24 THREE-DIMENSIONAL SCAFFOLD CONSTITUTED BY FIBROIN AND POLYETHYLENE GLUTEN FOR CELL CULTURE

Publications (2)

Publication Number Publication Date
WO2013111065A1 WO2013111065A1 (en) 2013-08-01
WO2013111065A8 true WO2013111065A8 (en) 2013-10-24

Family

ID=45998466

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2013/050577 WO2013111065A1 (en) 2012-01-24 2013-01-23 Three-dimensional scaffold consisting of fibroin and polyethylene useful for cell cultures

Country Status (2)

Country Link
IT (1) ITMI20120076A1 (en)
WO (1) WO2013111065A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107469147A (en) * 2017-08-14 2017-12-15 浙江大学 A kind of silk collagen scaffold of biotic factor release and preparation method and application

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112111162B (en) * 2019-06-21 2022-03-08 中国科学院苏州纳米技术与纳米仿生研究所 Rapidly-curable double-network hydrogel and preparation method and application thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5640156A (en) * 1979-09-05 1981-04-16 Kanebo Ltd Porous membrane and its manufacture
JPS5653678A (en) 1980-06-25 1981-05-13 Taisho Pharmaceut Co Ltd Preparation of 1,10-phenanthroilnes
ITVR20010098A1 (en) * 2001-09-11 2003-03-11 Consorzio Per Gli Studi Uni PROCEDURE FOR OBTAINING SILK FIBROIN HYDROGELS.
WO2004062697A2 (en) * 2003-01-07 2004-07-29 Tufts University Silk fibroin materials and use thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107469147A (en) * 2017-08-14 2017-12-15 浙江大学 A kind of silk collagen scaffold of biotic factor release and preparation method and application

Also Published As

Publication number Publication date
ITMI20120076A1 (en) 2013-07-25
WO2013111065A1 (en) 2013-08-01

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