WO2013096642A1 - Heteroaryls and uses thereof - Google Patents

Heteroaryls and uses thereof Download PDF

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Publication number
WO2013096642A1
WO2013096642A1 PCT/US2012/070988 US2012070988W WO2013096642A1 WO 2013096642 A1 WO2013096642 A1 WO 2013096642A1 US 2012070988 W US2012070988 W US 2012070988W WO 2013096642 A1 WO2013096642 A1 WO 2013096642A1
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WIPO (PCT)
Prior art keywords
nitrogen
optionally substituted
membered
sulfur
oxygen
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PCT/US2012/070988
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French (fr)
Inventor
Ryan W. CHAU
Courtney A. Cullis
Matthew O. Duffey
Krista E. GIPSON
Yongbo Hu
Gang Li
Michael D. Sintchak
Tricia J. Vos
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Millennium Pharmaceuticals, Inc.
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Application filed by Millennium Pharmaceuticals, Inc. filed Critical Millennium Pharmaceuticals, Inc.
Priority to EP12859082.5A priority Critical patent/EP2793894A4/en
Priority to JP2014548902A priority patent/JP2015503505A/en
Publication of WO2013096642A1 publication Critical patent/WO2013096642A1/en

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    • A61K31/437—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system containing a five-membered ring having nitrogen as a ring hetero atom, e.g. indolizine, beta-carboline
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    • C07D207/30—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members
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    • C07D207/36—Oxygen or sulfur atoms
    • C07D207/40—2,5-Pyrrolidine-diones
    • C07D207/416—2,5-Pyrrolidine-diones with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to other ring carbon atoms
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Definitions

  • Phosphatidylinositol 3-kinase is a family of lipid kinases that phosphorylate phosphatidylinositol at the 3' position of the inositol ring.
  • PI3K is comprised of several classes of genes, including Class IA, IB, II and III and some of these classes contain several isoforms (reviewed in Engelman et al., Nature Review Genetics 7:606-619 (2006)). Adding to the complexity of this family is the fact that PI3Ks function as heterodimers, comprising a catalytic domain and a regulatory domain.
  • PI3K family is structurally related to a larger group of lipid and serine/threonine protein kinases known as the phosphatidylinositol 3-kinase like kinases (PIKKs), which also includes DNA-PK, ATM, ATR, mTOR, TRRAP and SMG1.
  • PIKKs phosphatidylinositol 3-kinase like kinases
  • PI3K is activated downstream of various mitogenic signals mediated through receptor tyrosine kinases, and subsequently stimulates a variety of biological outcomes; including increased cell survival, cell cycle progression, cell growth, cell metabolism, cell migration and angiogenesis (reviewed in Cantley, Science 296: 1655-57 (2002); Hennessy et al., Nature Reviews Drug Discovery 4:988-1004 (2005); Engelman et al., Nature Review Genetics 7:606-619 (2006)).
  • PI3K hyper-activation is associated with a number of hyper-proliferative, inflammatory, or cardiovascular disorders; including cancer, inflammation, and cardiovascular disease.
  • the molecules defined within this invention inhibit the activity of PI3K, and therefore may be useful for the treatment of proliferative, inflammatory, or cardiovascular disorders.
  • Cases where PI3K pathway mutations have been linked to proliferative disorders where the molecules defined within this invention may have a therapeutic benefit include benign and malignant tumors and cancers from diverse lineage, including but not limited to those derived from colon (Samuels et al., Science 304:554 (2004); reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006)), liver (reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006)), intestine (reviewed in Hennessy et al., Nature Reviews Drug Discovery 4:988- 1004 (2005)), stomach (Samuels et al., Science 304:554 (2004); reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006)), esophagus (Phillips et
  • Other classes of disorders with aberrant PI3K pathway signaling where the molecules defined within this invention may have a therapeutic benefit include inflammatory and cardiovascular diseases, including but not limited to allergies/anaphylaxis (reviewed in Rommel et al., Nature Reviews Immunology 7: 191 -201 (2007)), acute and chronic inflammation (reviewed in Ruckle et al., Nature Reviews Drug Discovery 5:903- 12 (2006); reviewed in Rommel et al., Nature Reviews Immunology 7: 191-201 (2007)), rheumatoid arthritis (reviewed in Rommel et al., Nature Reviews Immunology 7: 191 - 201 (2007)); autoimmunity disorders (reviewed in Ruckle et al., Nature Reviews Drug Discovery 5:903- 12 (2006)), thrombosis (Jackson et al., Nature Medicine 1 1 :507- 14 (2005); reviewed in Ruckle et al., Nature Reviews Drug Discovery 5:903- 12 (2006)), hypertension (reviewed in Ruckle et al.
  • Vacuolar Protein Sorting 34 is the sole Class III PI3K family member. VPS34 functions in the formation and trafficking of multiple intracellular vesicles, including vacuoles, endosomes, multivessicular bodies, lysosomes and autophagosomes (reviewed in Backer Biochem J 2008; Yan and Backer Biochem J 2007). VPS34 carries out these activities by phosphorylating Ptdlns forming PtdIns3P, resulting in the recruitment and localization of a variety of FYVE and PX domain containing effector proteins that facilitate vesicular formation, elongation and movement. At a cellular level, inhibition of VPS34 results in defects in protein sorting and autophagy.
  • autophagy is a regulated process whereby cells catabolize subcellular components targeted for degradation by enclosing them in double -membrane vesicles which then fuse with lysosomes.
  • Autophagy has been best characterized as occurring during times of nutrient deprivation, but also plays a role in normal cellular and tissue homeostasis and functions, including the development of multiple tissue types, the immune response, clearance of neuronal aggregates and tumor suppression.
  • VPS34 may also participate in several signal transduction pathways (reviewed in Backer Biochem J 2008).
  • VPS34 plays an important role in many critical cellular processes including autophagy
  • inhibitors of VPS34 may have therapeutic application in a number of diseases, including but not limited to cancer, muscular disorders, neurodegeneration, inflammatory disease, infectious disease and other age related illnesses (reviewed in Shintani and Klionsky Science 2004; Kondo et al Nat Rev Cancer 2005; Delgato et al Immunol Rev 2009).
  • VPS34 and/or PI3K inhibitors that possess good therapeutic properties, especially for the treatment of proliferative, inflammatory, or cardiovascular disorders.
  • This invention provides compounds that are inhibitors of VPS34 and/or PI3K, and accordingly are useful for the treatment of proliferative, inflammatory, or cardiovascular disorders.
  • the compounds of this invention are represented by formula IA:
  • each occurrence of R 3 is independently hydrogen, -CN, halogen, -Z-R 5 , or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
  • Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
  • R 3a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3-10- membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R 4 is -Z 2 -R 6 wherein:
  • Z 2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-,
  • R 4a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH 2 , 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R , taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R 2 is optionally substituted with 1 -4 occurrences of R 2a , wherein each occurrence of R 2a is independently -R 12a , -T 2 -R 12d , -T 2 -R 12a , or
  • each occurrence of R 12a is independently halogen, -CN, -N0 2 , -R 12c , -N(R 12b ) 2 ,
  • heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; each occurrence of R 12b is independently hydrogen or an optionally substituted group selected from ⁇ .
  • each occurrence of R 12c is independently an optionally substituted group selected from Ci_ aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12e is independently hydrogen or an optionally substituted
  • each occurrence of V 2 is independently -N(R 12e )-, -0-, -S-, -S(O)-,
  • T 2 is an optionally substituted ⁇ . alkylene chain wherein the alkylene chain optionally is interrupted by -N(R 13 )-, -0-, -S-, -S(0)-, -S(0) 2 -, -C(0)-, -C(0)0-, -C(0)N(R 13 )-,
  • HY is an optionally substituted group selected from:
  • each occurrence of 3 ⁇ 4, X 5 , ⁇ , ⁇ , and Xg is independently -CR 10 or N, provided no more than one occurrence of X4, X5, ⁇ , X7, and Xg is N, and at least two occurrences of CR 10 are CH;
  • each occurrence of Qi and Q 2 is independently S, O or -NR 9 ;
  • each occurrence of Yi, Y 2 , Y3, Y 4 , Y5, ⁇ , Y7, and Yg is -CR 10 ;
  • R 10 is -R 10b , -V R 10c , -T R 10b , or -Vi-Ti-R 10b wherein:
  • Vi is -NR 11 -, -NR u -C(0)-, -NR U -C(S)-, -NR U -C(NR U )-, -NR u C(0)0-, - NR u C(0)NR u -, -NR u C(0)S-, -NR u C(S)0-, -NR U C(S)NR U -, -NR U C(S)S-, - NR u C(NR u )0-, -NR 11 CfNR 1 ⁇ NR 11 -, -NR u S(0) 2 -, -NR u S(0) 2 NR u -, -C(O)-, -C0 2 -, - C(0)NR u -, -C(0)NR u O-, -SO 2 -, or -S0 2 NR u -;
  • each occurrence of R 10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • Ti is an optionally substituted ⁇ . alkylene chain wherein the alkylene chain optionally is interrupted by -N(R U )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(0)-, -C(0)0-, - C(0)N(R u )-, -S(0) 2 N(R u )-, -OC(0)N(R u )-, -N(R u )C(0)-, -N(R u )S0 2 -, - N(R lla )C(0)0-, -N(R 10a )C(O)N(R 10a )-, -N(R 10a )S(O) 2 N(R 10a )-, -OC(O)-, or -C(0)N(R u )-
  • each occurrence of R 10b is independently hydrogen, halogen, -CN, -N0 2 , -N(R U ) 2 , -OR 10a , -SR 10a , -S(O) 2 R 10a , -C(O)R 10a , -C(O)OR 10a , -C(0)N(R u ) 2 , -S(0) 2 N(R u ) 2 , -OC(0)N(R u ) 2 , -N(R u )C(O)R 10a , -N(R u )SO 2 R 10a , -N(R u )C(O)OR 10a , - N(R u )C(0)N(R u ) 2 , or -N(R u )S0 2 N(R u ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4-
  • each occurrence of R 10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
  • R 10a and R 1 ⁇ 3 ⁇ 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 11 is independently hydrogen, -C(0)R l la , -C0 2 R l la , -C(0)N(R l la ) 2 ,
  • Ci_ 6 aliphatic 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R l la is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each occurrence of R 9 is independently hydrogen, -C(0)R 9a , -C0 2 R 9a , -C(0)N(R 9b ) 2 , -S0 2 R 9a , - S0 2 N(R 9b ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
  • cycloaliphatic 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1 -5 heteratoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R % taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 1 is not an unsubstituted phenyl or a phenyl substituted only with one or two groups selected from methyl, tert-butyl, -CF 3 or halogen;
  • R 1 , R 2 , and Hy are not all simultaneously pyridyl
  • Hy is not 5-methyl-3-phenyl-4-isoxazolyl
  • R 1 and R 2 when one of R 1 and R 2 is cyclopropyl and the other is optionally substituted phenyl, and R 3 is - C(0)-R 5 or -C(0)OR 4 , then R 5 is not optionally substituted piperidinyl and R 4 is not ethyl; e) neither R 1 nor R 2 is a phenyl ring substituted with optionally substituted lH-Pyrrolo[2,3- b]pyridinyl or 0-CH 2 -phenyl. f) neither R 1 nor R 2 is an unsubstituted ring selected from indolyl, or an optionally substituted ring selected from:
  • R 1 and R 2 are not both optionally substituted cyclopropyl
  • R 1 is not a phenyl ring substituted with optionally substituted phenyl, -C(0)-phenyl, -NH-CH 2 - phenyl, or -O-phenyl; or an unsubstituted ring selected from quinolinyl, dibenzoftiran, naphthyl, dibenzothiophene; or an optionally substituted ring selected from indolyl, 3H-Imidazo[4,5- b]pyridinyl, or:
  • R 1 is not a pyrimidinyl or pyridinyl ring substituted with-N(H)C(H)(R 20 )-phenyl, wherein R 20 is hydrogen or methyl;
  • R 1 is not -C(0)NHR 21 , wherein R 21 is thiazole, or -C(0)N(H)C(H)(Et)-R 22 or -C(H)(Et)-R 22 , wherein R 22 is pyridinyl or phenyl;
  • R 2 is not a phenyl ring substituted with optionally substituted -NH-CH 2 -pyridinyl, -NH-CH 2 - phenyl, -NH-CH 2 -2,3-dihydro-lH-Indene, or -NH-C(0)-phenyl;
  • each occurrence of R 14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
  • each occurrence of R 3 is independently hydrogen, -CN, halogen, -Z-R 5 , or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
  • Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
  • R 3a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3- 10- membered cycloaliphatic, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R 4 is -Z 2 -R 6 wherein:
  • Z 2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-, -S(0) 2 -, -C(O)-, -CO 2 -, -C(0)NR 4 ⁇ -C(NH)-, or -S(0) 2 NR 4 ⁇
  • R 4a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH 2 , 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
  • R 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R 2 is optionally substituted with 1 -4 occurrences of R 2a , wherein each occurrence of R 2a is independently -R 12a , -T 2 -R 12d , -T 2 -R 12a , or
  • each occurrence of R 12a is independently halogen, -CN, -N0 2 , -R 12c , -N(R 12b ) 2 ,
  • each occurrence of R 12b is independently hydrogen or an optionally substituted group selected from Ci_C 6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12c is independently an optionally substituted group selected from Ci_ aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12e is independently hydrogen or an optionally substituted
  • each occurrence of V 2 is independently -N(R 12e )-, -0-, -S-, -S(O)-,
  • T 2 is an optionally substituted ⁇ . alkylene chain wherein the alkylene chain optionally is interrupted by -N(R 13 )-, -0-, -S-, -S(0)-, -S(0) 2 -, -C(0)-, -C(0)0-, -C(0)N(R 13 )-,
  • HY is an o tionall substituted rou selected from:
  • each occurrence of X4, X 5 , ⁇ , ⁇ , and Xg is independently -CR 10 or N, provided no more than one occurrence of X4, X 5 , ⁇ , X7, and Xg is N, and at least two occurrences of CR 10 are CH; each occurrence of Qi and Q 2 is independently S, O or -NR 9 ;
  • each occurrence of Yi, Y 2 , Y3, Y 4 , Y5, ⁇ , Y7, and Yg is -CR 10 ;
  • R 10 is -R 10b , -V R 10c , -T R 10b , or -Vi-Ti-R 10b wherein:
  • Vi is -NR 11 -, -NR u -C(0)-, -NR U -C(S)-, -NR U -C(NR U )-, -NR u C(0)0-, - NR u C(0)NR u -, -NR u C(0)S-, -NR u C(S)0-, -NR U C(S)NR U -, -NR U C(S)S-, - NR u C(NR u )0-, -NR 11 CfNR 1 ⁇ NR 11 -, -NR u S(0) 2 -, -N ⁇ NR 11 -, -C(O)-, -C0 2 -, - C(0)NR u -, -C(0)NR u O-, -SO 2 -, or -S0 2 NR u -;
  • each occurrence of R 10a is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • Ti is an optionally substituted C C alkylene chain wherein the alkylene chain optionally is interrupted by -N(R U )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(O)-, -C(0)0-, - C(0)N(R u )-, -S(0) 2 N(R u )-, -OC(0)N(R u )-, -N(R u )C(0)-, -N(R u )S0 2 -, - N(R lla )C(0)0-, -N(R 10a )C(O)N(R 10a )-, -N(R 10a )S(O) 2 N(R 10a )-, -OC(O)-, or -C(0)N(R u )-,
  • each occurrence of R 10b is independently hydrogen, halogen, -CN, -N0 2 , -N(R U ) 2 , -OR 10a , -SR 10a , -S(O) 2 R 10a , -C(O)R 10a , -C(O)OR 10a , -C(0)N(R u ) 2 , -S(0) 2 N(R u ) 2 , -OC(0)N(R u ) 2 , -N(R u )C(O)R 10a , -N(R u )SO 2 R 10a , -N(R u )C(O)OR 10a , - N(R u )C(0)N(R u ) 2 , or -N(R u )S0 2 N(R u ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4-
  • each occurrence of R 10c is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
  • R 10a and R 1 ⁇ 3 ⁇ 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 11 is independently hydrogen, -C(0)R l la , -C0 2 R Ua , -C(0)N(R l la ) 2 ,
  • each occurrence of R l la is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3-10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9 is independently hydrogen, -C(0)R 9a , -C0 2 R 9a , -C(0)N(R 9b ) 2 , -S0 2 R 9a , - S0 2 N(R 9b ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
  • cycloaliphatic 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1 -5 heteratoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R % taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein a substituent on HY and R , taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; and
  • R 1 is not an unsubstituted phenyl or a phenyl substituted only with one or two groups selected from methyl, tert-butyl, -CF 3 or halogen;
  • R 1 , R 2 , and Hy are not all simultaneously pyridyl
  • Hy is not 5-methyl-3-phenyl-4-isoxazolyl
  • R 1 and R 2 when one of R 1 and R 2 is cyclopropyl and the other is optionally substituted phenyl, and R 3 is -C(0)-R 5 or -C(0)OR 4 , then R 5 is not optionally substituted piperidinyl and R 4 is not ethyl; e) neither R 1 nor R 2 is a phenyl ring substituted with optionally substituted lH-Pyrrolo[2,3- b]pyridinyl or 0-CH 2 -phenyl.
  • R 1 nor R 2 is an unsubstituted ring selected from indolyl, or an optionally substituted
  • R 1 and R 2 are not both optionally substituted cyclopropyl
  • R 1 and R 2 are both optionally substituted phenyl, Hy is not optionally substituted quinolinyl or acridinyl;
  • R 1 is not a phenyl ring substituted with optionally substituted phenyl, -C(0)-phenyl, -NH- CH 2 -phenyl, or -O-phenyl; or an unsubstituted ring selected from quinolinyl, dibenzofuran, naphthyl, dibenzothiophene; or an optionally substituted ring selected from indolyl, 3H- Imidazo[4,5-b]pyridinyl, or:
  • R 1 is not a pyrimidinyl or pyridinyl ring substituted with-N(H)C(H)(R 20 )-phenyl, wherein R is hydrogen or methyl; k) R 1 is not -C(0)NHR 21 , wherein R 21 is thiazole, or -C(0)N(H)C(H)(Et)-R 22 or -C(H)(Et)-R 22 , wherein R 22 is pyridinyl or phenyl;
  • R 2 is not a phenyl ring substituted with optionally substituted -NH-CH 2 -pyridinyl, -NH-CH 2 - phenyl, -NH-CH 2 -2,3-dihydro-lH-Indene, or -NH-C(0)-phenyl;
  • each occurrence of R 14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
  • each occurrence of R 3 is independently hydrogen, -CN, halogen, -Z-R 5 , or an optionally substituted group selected from Ci_ 6 aliphatic and 3-10-membered cycloaliphatic, wherein:
  • Z is selected from an optionally substituted Ci_ 3 alkylene chain, -0-,
  • R 3a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 41 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 4 is hydrogen, -OH, or an optionally substituted group selected from Ci_ 6 aliphatic, 3-10- membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R 4 is -Z 2 -R 6 wherein:
  • Z 2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-,
  • R 4a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH 2 , 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
  • R 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4-10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R 2 is optionally substituted with 1 -4 occurrences of R 2a , wherein each occurrence of R 2a is independently -R 12a , -T 2 -R 12d , -T 2 -R 12a , or
  • each occurrence of R 12a is independently halogen, -CN, -N0 2 , -R , -N(R 12b ) 2 , -OR 12b , -SR 12c , -S(0) 2 R 12c , -C(0)R 12b , -C(0)OR 12b , -C(0)N(R 12b ) 2 , -S(0) 2 N(R 12b ) 2 ,
  • heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; each occurrence of R 12b is independently hydrogen or an optionally substituted group selected from ⁇ .
  • each occurrence of R 12c is independently an optionally substituted group selected from Ci_ C 6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12e is independently hydrogen or an optionally substituted
  • each occurrence of V 2 is independently -N(R 12e )-, -0-, -S-, -S(O)-,
  • T 2 is an optionally substituted ⁇ . alkylene chain wherein the alkylene chain optionally is interrupted by -N(R 13 )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(O)-, -C(0)0-, -C(0)N(R 13 )-,
  • HY is an optionally substituted group selected from:
  • each occurrence of X4, X 5 , ⁇ , X7, and Xg is independently -CR or N, provided no more than one occurrence of X4, X 5 , ⁇ , X7, and Xg is N, and at least two occurrences of CR 10 are CH;
  • each occurrence of Qi and Q 2 is independently S, O or -NR 9 ;
  • each occurrence of Yi, Y 2 , Y3, Y 4 , Y5, ⁇ , Y7, and Yg is -CR 10 ;
  • R is -R -V R , -T R 1Ub , or -Vi-Ti-R 1Ub wherein:
  • Vi is -NR 11 -, -NR u -C(0)-, -NR U -C(S)-, -NR U -C(NR U )-, -NR u C(0)0-, - NR u C(0)NR u -, -NR u C(0)S-, -NR u C(S)0-, -NR U C(S)NR U -, -NR U C(S)S-, - NR u C(NR u )0-, -NR 11 CfNR 1 ⁇ NR 11 -, -NR u S(0) 2 -, -NR ⁇ CO ⁇ NR 11 -, -C(O)-, -C0 2 -, - C(0)NR u -, -C(0)NR u O-, -S0 2 -, or -S0 2 NR u -;
  • each occurrence of R 10a is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • Ti is an optionally substituted Ci_C6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R U )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(O)-, -C(0)0-, - C(0)N(R u )-, -S(0) 2 N(R u )-, -OC(0)N(R u )-, -N(R u )C(0)-, -N(R u )S0 2 -, - N(R lla )C(0)0-, -N(R 10a )C(O)N(R 10a )-, -N(R 10a )S(O) 2 N(R 10a )-, -OC(O)-, or -C(0)N(R u )- O- or wherein Ti forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl
  • each occurrence of R 10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
  • R 10a and R 1 ⁇ 3 ⁇ 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 11 is independently hydrogen, -C(0)R l la , -C0 2 R l la , -C(0)N(R l la ) 2 ,
  • each occurrence of R l la is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3-10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9 is independently hydrogen, -C(0)R 9a , -C0 2 R 9a , -C(0)N(R 9b ) 2 , -S0 2 R 9a , - S0 2 N(R 9b ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
  • cycloaliphatic 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9a is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1 -5 heteratoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R % taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 1 is not an unsubstituted phenyl or a phenyl substituted only with one or two groups selected from methyl, tert-butyl, -CF 3 or halogen;
  • R 1 , R 2 , and Hy are not all simultaneously pyridyl
  • Hy is not 5-methyl-3-phenyl-4-isoxazolyl
  • R 1 and R 2 when one of R 1 and R 2 is cyclopropyl and the other is optionally substituted phenyl, and R 3 is -C(0)-R 5 or -C(0)OR 4 , then R 5 is not optionally substituted piperidinyl and R 4 is not ethyl; e) neither R 1 nor R 2 is a phenyl ring substituted with optionally substituted lH-Pyrrolo[2,3- b]pyridinyl or 0-CH 2 -phenyl.
  • R 1 nor R 2 is an unsubstituted ring selected from indolyl, or an optionally substituted
  • R 1 and R 2 are not both optionally substituted cyclopropyl
  • R 1 is not a phenyl ring substituted with optionally substituted phenyl, -C(0)-phenyl, -NH- CH 2 -phenyl, or -O-phenyl; or an unsubstituted ring selected from quinolinyl, dibenzofuran, naphthyl, dibenzothiophene; or an optionally substituted ring selected from indolyl, 3H- Imidazo[4,5-b]pyridinyl, or:
  • R 1 is not a pyrimidinyl or pyridinyl ring substituted with-N(H)C(H)(R 20 )-phenyl, wherein R 21 is hydrogen or methyl;
  • R 1 is not -C(0)NHR 21 , wherein R 21 is thiazole, or -C(0)N(H)C(H)(Et)-R 22 or -C(H)(Et)-R 22 , wherein R 22 is pyridinyl or phenyl;
  • R 2 is not a phenyl ring substituted with optionally substituted -NH-CH 2 -pyridinyl, -NH-CH 2 - phenyl, -NH-CH 2 -2,3-dihydro-lH-Indene, or -NH-C(0)-phenyl;
  • each occurrence of R 14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
  • each occurrence of R 3 is independently hydrogen, -CN, halogen, -Z-R 5 , or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
  • Z is selected from an optionally substituted Ci_ 3 alkylene chain, -0-,
  • R 3a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3-10- membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R 4 is -Z 2 -R 6 wherein:
  • Z 2 is selected from an optionally substituted Ci_ 3 alkylene chain, -S(O)-,
  • R a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH 2 , 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
  • R 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R 2 is optionally substituted with 1 -4 occurrences of R 2a , wherein each occurrence of R 2a is independently -R 12a , -T 2 -R 12d , -T 2 -R 12a , or
  • each occurrence of R 12a is independently halogen, -CN, -N0 2 , -R 12c , -N(R 12b ) 2 ,
  • heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; each occurrence of R 12b is independently hydrogen or an optionally substituted group selected from ⁇ .
  • each occurrence of R 12c is independently an optionally substituted group selected from Ci_ aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12e is independently hydrogen or an optionally substituted
  • each occurrence of V 2 is independently -N(R e )-, -0-, -S-, -S(O)-,
  • T 2 is an optionally substituted ⁇ . alkylene chain wherein the alkylene chain optionally is interrupted by -N(R 13 )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(O)-, -C(0)0-, -C(0)N(R 13 )-,
  • each occurrence of X 4 , X 5 , X 6 , X7, and X 8 is independently -CR 10 or N, provided no more than one occurrence of X 4 , X 5 , X 6 , X7, and Xg is N, and at least two occurrences of CR 10 are CH;
  • each occurrence of Qi and Q 2 is independently S, O or -NR 9 ;
  • each occurrence of Yi, Y 2 , Y3, Y 4 , Y5, ⁇ , ⁇ , and Yg is -CR 10 ;
  • R 10 is -R 10b , -V R 10c , -T R 10b , or -V T R 1015 wherein: Vi is -NR 11 -, -NR u -C(0)-, -NR U -C(S)-, -NR U -C(NR U )-, -NR u C(0)0-, - NR u C(0)NR u -, -NR u C(0)S-, -NR u C(S)0-, -NR U C(S)NR U -, -NR U C(S)S-, - NR u C(NR u )0-, -NR 11 CfNR 1 ⁇ NR 11 -, -NR u S(0) 2 -, -N ⁇ NR 11 -, -C(O)-, -C0 2 -, - C(0)NR u -, -C(0)NR u O-, -SO 2 -, or -S0 2 NR
  • each occurrence of R 10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • Ti is an optionally substituted ⁇ . alkylene chain wherein the alkylene chain optionally is interrupted by -N(R U )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(O)-, -C(0)0-, - C(0)N(R u )-, -S(0) 2 N(R u )-, -OC(0)N(R u )-, -N(R u )C(0)-, -N(R u )S0 2 -, - N(R lla )C(0)0-, -N(R 10a )C(O)N(R 10a )-, -N(R 10a )S(O) 2 N(R 10a )-, -OC(O)-, or -C(0)N(R u )-
  • each occurrence of R 10b is independently hydrogen, halogen, -CN, -N0 2 , -N(R U ) 2 , -OR 10a , -SR 10a , -S(O) 2 R 10a , -C(O)R 10a , -C(O)OR 10a , -C(0)N(R u ) 2 , -S(0) 2 N(R u ) 2 , -OC(0)N(R u ) 2 , -N(R u )C(O)R 10a , -N(R u )SO 2 R 10a , -N(R u )C(O)OR 10a , - N(R u )C(0)N(R u ) 2 , or -N(R u )S0 2 N(R u ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4-
  • each occurrence of R 10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
  • R 10a and R 1 ⁇ 3 ⁇ 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 11 is independently hydrogen, -C(0)R l la , -C0 2 R l la , -C(0)N(R l la ) 2 ,
  • each occurrence of R l la is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3-10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9 is independently hydrogen, -C(0)R 9a , -C0 2 R 9a , -C(0)N(R 9b ) 2 , -S0 2 R 9a , - S0 2 N(R 9b ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
  • cycloaliphatic 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9a is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1 -5 heteratoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R % taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 1 is not an optionally substituted phenyl
  • R 1 , R 2 , and Hy are not all simultaneously pyridyl
  • Hy is not 5-methyl-3-phenyl-4-isoxazolyl
  • R 1 when one of R 1 is cyclopropyl and R 2 is optionally substituted phenyl, and R 3 is -C(0)-R 5 or -C(0)OR 4 , then R 5 is not optionally substituted piperidinyl and R 4 is not ethyl;
  • R 2 is not a phenyl ring substituted with optionally substituted lH-Pyrrolo[2,3-b]pyridinyl or 0-CH 2 -phenyl;
  • R 1 nor R 2 is an unsubstituted ring selected from indolyl, or an optionally substituted ring selected from: g) R 1 and R 2 are not both optionally substituted cyclopropyl;
  • R 1 is not a pyrimidinyl or pyridinyl ring substituted with-N(H)C(H)(R 20 )-phenyl, wherein R 21 is hydrogen or methyl;
  • R 1 is not -C(0)NHR 21 , wherein R 21 is thiazole, or -C(0)N(H)C(H)(Et)-R 22 or -C(H)(Et)-R 22 , wherein R 22 is pyridinyl or phenyl;
  • R 2 is not a phenyl ring substituted with optionally substituted -NH-CH 2 -pyridinyl, -NH-CH 2 - phenyl, -NH-CH 2 -2,3-dihydro-lH-Indene, or -NH-C(0)-phenyl;
  • each occurrence of R 14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
  • each occurrence of R 3 is independently hydrogen, -CN, halogen, -Z-R 5 , or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
  • Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
  • R 3a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3-10- membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R 4 is -Z 2 -R 6 wherein:
  • Z 2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-,
  • R 4a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH 2 , 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
  • R 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4-10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R 2 is optionally substituted with 1 -4 occurrences of R 2a , wherein each occurrence of R 2a is independently -R 12a , -T 2 -R 12d , -T 2 -R 12a , or
  • each occurrence of R 12a is independently halogen, -CN, -N0 2 , -R 12c , -N(R 12b ) 2 ,
  • each occurrence of R 12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12e is independently hydrogen or an optionally substituted
  • each occurrence of V 2 is independently -N(R 12e )-, -0-, -S-, -S(O)-,
  • T 2 is an optionally substituted Ci_C 6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R 13 )-, -0-, -S-, -S(0)-, -S(0) 2 -, -C(0)-, -C(0)0-, -C(0)N(R 13 )-,
  • HY is an o tionall substituted rou selected from:
  • each occurrence of X4, X 5 , ⁇ , ⁇ , and Xg is independently -CR 10 or N, provided no more than one occurrence of X4, X 5 , ⁇ , X7, and Xg is N, and at least two occurrences of CR 10 are CH;
  • each occurrence of Qi and Q 2 is independently S, O or -NR 9 ;
  • each occurrence of Yi, Y 2 , Y3, Y 4 , Y 5 , ⁇ , Y7, and Yg is -CR 10 ; or wherein two adjacent occurrences of X4 and X 5 , Xe and X 7 , X 7 and Xg, Yi and Qi, Y3 and Q2, or Y 4 and Y 5 , taken together with the atom to which they are bound, form an optionally substituted fused group selected from 5-6-membered aryl, or 5-6-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 10 is -R 10b , -V R 10c , -T R 10b , or -V T R 1015 wherein:
  • Vi is -NR 11 -, -NR u -C(0)-, -NR U -C(S)-, -NR U -C(NR U )-, -NR u C(0)0-, - NR u C(0)NR u -, -NR u C(0)S-, -NR u C(S)0-, -NR U C(S)NR U -, -NR U C(S)S-, - NR u C(NR u )0-, -NR 11 CfNR 1 ⁇ NR 11 -, -NR u S(0) 2 -, -N ⁇ NR 11 -, -C(O)-, -C0 2 -, - C(0)NR u -, -C(0)NR u O-, -SO 2 -, or -S0 2 NR u -;
  • each occurrence of R 10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • Ti is an optionally substituted Ci_C 6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R U )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(O)-, -C(0)0-, - C(0)N(R u )-, -S(0) 2 N(R u )-, -OC(0)N(R u )-, -N(R u )C(0)-, -N(R u )S0 2 -, - N(R lla )C(0)0-, -N(R 10a )C(O)N(R 10a )-, -N(R 10a )S(O) 2 N(R 10a )-, -OC(O)-, or -C(0)N(R u )-,
  • each occurrence of R 10b is independently hydrogen, halogen, -CN, -N0 2 , -N(R U ) 2 , -OR 10a , -SR 10a , -S(O) 2 R 10a , -C(O)R 10a , -C(O)OR 10a , -C(0)N(R u ) 2 , -S(0) 2 N(R u ) 2 , -OC(0)N(R u ) 2 , -N(R u )C(O)R 10a , -N(R u )SO 2 R 10a , -N(R u )C(O)OR 10a , - N(R u )C(0)N(R u ) 2 , or -N(R u )S0 2 N(R u ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4-
  • each occurrence of R 10c is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or R a and R ; taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 11 is independently hydrogen, -C(0)R l la , -C0 2 R Ua , -C(0)N(R l la ) 2 ,
  • each occurrence of R l la is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3-10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9 is independently hydrogen, -C(0)R 9a , -C0 2 R 9a , -C(0)N(R 9b ) 2 , -S0 2 R 9a , - S0 2 N(R 9b ) 2 , or an optionally substituted group selected from Ci_ 6 aliphatic, 3-10-membered
  • cycloaliphatic 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1 -5 heteratoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R % taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • a substituent on HY and R 14 taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; and provided that R is not an unsubstituted phenyl or a phenyl substituted only with one or two groups selected from methyl, tert-butyl, -CF 3 or halogen; and
  • R 1 , R 2 , and Hy are not all simultaneously pyridyl
  • Hy is not 5-methyl-3-phenyl-4-isoxazolyl
  • R 1 and R 2 when one of R 1 and R 2 is cyclopropyl and the other is optionally substituted phenyl, and R 3 is -C(0)-R 5 or -C(0)OR 4 , then R 5 is not optionally substituted piperidinyl and R 4 is not ethyl; e) neither R 1 nor R 2 is a phenyl ring substituted with optionally substituted lH-Pyrrolo[2,3- b]pyridinyl or 0-CH 2 -phenyl.
  • R 1 nor R 2 is an unsubstituted ring selected from indolyl, or an optionally substituted
  • R 1 and R 2 are not both optionally substituted cyclopropyl
  • R 1 and R 2 are both optionally substituted phenyl, Hy is not optionally substituted quinolinyl or acridinyl;
  • R 1 is not a phenyl ring substituted with optionally substituted phenyl, -C(0)-phenyl, -NH- CH 2 -phenyl, or -O-phenyl; or an unsubstituted ring selected from quinolinyl, dibenzofuran, naphthyl, dibenzothiophene; or an optionally substituted ring selected from indolyl, 3H- Imidazo[4,5-b]pyridinyl, or:
  • R 1 is not a pyrimidinyl or pyridinyl ring substituted with-N(H)C(H)(R 20 )-phenyl, wherein R 21 is hydrogen or methyl;
  • R 1 is not -C(0)NHR 21 , wherein R 21 is thiazole, or -C(0)N(H)C(H)(Et)-R 22 or -C(H)(Et)-R 22 , wherein R 22 is pyridinyl or phenyl;
  • R 2 is not a phenyl ring substituted with optionally substituted -NH-CH 2 -pyridinyl, -NH-CH 2 - phenyl, -NH-CH 2 -2,3-dihydro-lH-Indene, or -NH-C(0)-ph ⁇ the compound is other than:
  • R 14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
  • each occurrence of R 3 is independently hydrogen, -CN, halogen, -Z-R 5 , or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
  • Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
  • R 3a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3-10- membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R 4 is -Z 2 -R 6 wherein:
  • Z 2 is selected from an optionally substituted Ci_ 3 alkylene chain, -S(O)-, -S(0) 2 -, -C(O)-, -C0 2 -, -C(0)NR 4 ⁇ -C(NH)-, or -S(0) 2 NR 4 ⁇
  • R 4a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH 2 , 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
  • R 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4-10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R 2 is optionally substituted with 1 -4 occurrences of R 2a , wherein each occurrence of R 2a is independently -R 12a , -T 2 -R 12d , -T 2 -R 12a , or
  • each occurrence of R 12a is independently halogen, -CN, -N0 2 , -R 12c , -N(R 12b ) 2 ,
  • heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; each occurrence of R 12b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12c is independently an optionally substituted group selected from Ci_ 6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12e is independently hydrogen or an optionally substituted
  • each occurrence of V 2 is independently -N(R 12e )-, -0-, -S-, -S(O)-,
  • T 2 is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R 13 )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(O)-, -C(0)0-, -C(0)N(R 13 )-,
  • HY is an optionally substituted group selected from:
  • each occurrence of X4, X 5 , ⁇ , X7, and Xg is independently -CR or N, provided no more than one occurrence of X4, X 5 , ⁇ , X7, and Xg is N, and at least two occurrences of CR 10 are CH;
  • each occurrence of Qi and Q 2 is independently S, O or -NR 9 ;
  • each occurrence of Yi, Y 2 , Y3, Y 4 , Y5, ⁇ , Y7, and Yg is -CR 10 ;
  • R is -R -V R , -T R 1Ub , or -Vi-Ti-R 1Ub wherein:
  • Vi is -NR 11 -, -NR -C(O)-, -NR -C(S)-, -NR -C(N U )-, -NR u C(0)0-, - NR u C(0)NR u -, -NR u C(0)S-, -NR u C(S)0-, -NR U C(S)NR U -, -NR U C(S)S-, - NR u C(NR u )0-, -NR 11 CfNR 1 ⁇ NR 11 -, -NR u S(0) 2 -, -NR ⁇ CO ⁇ NR 11 -, -C(O)-, -C0 2 -, - C(0)NR u -, -C(0)NR u O-, -SO2-, or -S0 2 NR u -;
  • Ti is an optionally substituted Ci_ 6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R U )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(O)-, -C(0)0-, - C(0)N(R u )-, -S(0) 2 N(R u )-, -OC(0)N(R u )-, -N(R u )C(0)-, -N(R u )S0 2 -, - N(R lla )C(0)0-, -N(R 10a )C(O)N(R 10a )-, -N(R 10a )S(O) 2 N(R 10a )-, -OC(O)-, or -C(0)N(R u )- O- or wherein Ti forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl
  • each occurrence of R 10a is independently hydrogen or an optionally substituted group selected from d_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each occurrence of R is independently hydrogen, halogen, -CN, -NO 2 ,
  • each occurrence of R 10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
  • R 10a and R 10b taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 11 is independently hydrogen, -C(0)R l la , -C0 2 R l la , -C(0)N(R l la ) 2 ,
  • each occurrence of R l la is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4-10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9 is independently hydrogen, -C(0)R 9a , -C0 2 R 9a , -C(0)N(R 9b ) 2 , -S0 2 R 9a , - S0 2 N(R 9b ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
  • cycloaliphatic 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9a is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R % taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 14 is independently hydrogen, cyclopropyl, or an optionally substituted group selected from Ci_6 aliphatic;
  • each occurrence of R 3 is independently hydrogen, -CN, halogen, -Z-R 5 , or an optionally substituted group selected from Ci_ 6 aliphatic and 3- to 10-membered cycloaliphatic, wherein:
  • Z is selected from an optionally substituted Ci_ 3 alkylene chain, -0-,
  • R 3a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 5 is hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10- membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each R 4 is independently selected from hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
  • R 4 is -Z 2 -R 6 wherein:
  • Z 2 is selected from an optionally substituted Ci_ 3 alkylene chain, -S(O)-, -S(0) 2 -, -C(O)-, -C0 2 -, -C(0)NR 4 ⁇ -C(NH)-, or -S(0) 2 NR 4 ⁇
  • R 4a is hydrogen or an optionally substituted Ci_ 4 aliphatic
  • R 6 is hydrogen, -NH 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
  • R 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 2 is hydrogen, halo, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10- membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R 2 is optionally substituted with 1-4 occurrences of R 2a , wherein each occurrence of R 2a is independently -R 12a , -T 2 -R 12d , -T 2 -R 12a , or -V 2 -T 2 -R 12d , and:
  • each occurrence of R 12a is independently halogen, -CN, -N0 2 , -R 12c , -N(R 12b ) 2 ,
  • each occurrence of R 12b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or two occurrences of R 12b , taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, Ci_6 haloaliphatic, 3- to 10-membered cycloaliphatic, 4- to 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12d is independently hydrogen or an optionally substituted group selected from 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12e is independently hydrogen or an optionally substituted
  • each occurrence of V 2 is independently -N(R 12e )-, -0-, -S-, -S(O)-,
  • T 2 is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R 13 )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(O)-, -C(0)0-, -C(0)N(R 13 )-,
  • HY is a group selected from:
  • each occurrence of X4, X 5 , X , X7, and Xg is independently -CR 10 , -CR 10 , or N, provided no more than two occurrences of X4, X 5 , ⁇ , X7, and Xg is N;
  • each occurrence of Yi, Y 2 , Y3, Y 4 , Y5, ⁇ , Y7, and Yg is -CR 10 ;
  • each occurrence of Qi and Q 2 is independently S, O or -NR 9 ;
  • X 4 and X 5 may be taken together with the atoms to which they are bound, to form an unsubstituted fused heteroaryl or heterocyclyl group having 8 to 10 ring atoms and having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 10 or R 10' is independently -R 10b , -V R 10c , -T R 10b , or -Vi-T R 10b , wherein:
  • Vi is -NR 11 -, -NR u -C(0)-, -NR U -C(S)-, -NR U -C(NR U )-, -NR u C(0)0-, - NR u C(0)NR u -, -NR u C(0)S-, -NR u C(S)0-, -NR U C(S)NR U -, -NR U C(S)S-, - NR u C(NR u )0-, -NR 11 CfNR 1 ⁇ NR 11 -, -NR u S(0) 2 -, -NR ⁇ CO ⁇ NR 11 -, -C(O)-, -C0 2 -, - C(0)NR u -, -C(0)NR u O-, -SO2-, or -S0 2 NR u -;
  • each occurrence of R 10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • Ti is an optionally substituted C e alkylene chain wherein the alkylene chain optionally is interrupted by -N(R U )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(O)-, -C(0)0-, - C(0)N(R u )-, -S(0) 2 N(R u )-, -OC(0)N(R u )-, -N(R u )C(0)-, -N(R u )S0 2 -, - N(R lla )C(0)0-, -N(R 10a )C(O)N(R 10a )-, -N(R 10a )S(O) 2 N(R 10a )-, -OC(O)-, or -C(0)N(R u )- O- or wherein Ti forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocycly
  • each occurrence of R 10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
  • R 10a and R 1 ⁇ 3 ⁇ 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 11 is independently hydrogen, -C(0)R l la , -C0 2 R l la , -C(0)N(R l la ) 2 ,
  • Ci_ 6 aliphatic 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R l la is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9 is independently hydrogen, -C(0)R 9a , -C0 2 R 9a , -C(0)N(R 9b ) 2 , -S0 2 R 9a , - S0 2 N(R 9b ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9a is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R % taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3- to 6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and provided that when HY is a non- fused group then HY is substituted with at least one occurrence of R 10 or R 10' , wherein R 10 or R 10' is:
  • V l is -NR 11 -
  • T t is a d -C 3 alkylene chain
  • R 10b is an optionally substituted 6- to 10-membered aryl ring or a 5- to 10-membered heteroaryl ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or Vi is -NR u C(0)NR u -
  • Ti is a Ci -C 3 alkylene chain
  • R 10b is -OR 10a ; or
  • Vi is -NR 11 -
  • R 10c is a 5- to 10-membered heteroaryl ring having 1- 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 3 when R 3 is hydrogen, then R 1 is not , wherein R 103 is hydrogen or -C(0) 2 tBu; when R 3 is hydrogen, then R 1 is not -CHzOCHzCHzSiMes;
  • R is an optionally substituted phenyl ring
  • R 1 is not a substituted pyrrolidinyl rins
  • each occurrence of R 3 is independently hydrogen, -CN, halogen, -Z-R 5 , or an optionally substituted group selected from Ci_6 aliphatic and 3- to 10-membered cycloaliphatic, wherein:
  • Z is selected from an optionally substituted Q_3 alkylene chain, -0-,
  • R 3a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 5 is hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10- membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each R 4 is independently selected from hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
  • R 4 is -Z 2 -R 6 wherein:
  • Z 2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-,
  • R 4a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 6 is hydrogen, -NH 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R , taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 2 is halo, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R 2 is optionally substituted with 1 -4 occurrences of R 2a , wherein each occurrence of R 2a is independently -R 12a , -T 2 -R 12d , -T 2 -R 12a , or -V 2 -T 2 -R 12d , and:
  • each occurrence of R 12a is independently halogen, -CN, -N0 2 , -R 12c , -N(R 12b ) 2 ,
  • each occurrence of R 12b is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or two occurrences of R 12b , taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, Ci_6 haloaliphatic, 3- to 10-membered cycloaliphatic, 4- to 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12d is independently hydrogen or an optionally substituted group selected from 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12e is independently hydrogen or an optionally substituted
  • Ci_6 aliphatic group each occurrence of V 2 is independently -N(R 12e )-, -0-, -S-, -S(0)-,
  • T 2 is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R 13 )-, -0-, -S-, -S(0)-, -S(0) 2 -, -C(O)-, -C(0)0-, -C(0)N(R 13 )-,
  • HY is a group selected from:
  • each occurrence of 3 ⁇ 4, X 5 , X 6 , X7, and X 8 is independently -CR 10 , -CR 10 , or N, provided no more than two occurrences of X4, X 5 , X 6 , X 7 , and X 8 is N;
  • each occurrence of Yi, Y 2 , Y 3 , Y 4 , Y 5 , Y 6 , Y 7 , and Y 8 is -CR 10 ;
  • each occurrence of Qi and Q 2 is independently S, O or -NR 9 ;
  • X4 and X 5 , Xe and X 7 , X 7 and Xg, Yi and -NR 9 , Y 3 and - NR 9 , or Y 4 and Y 5 may be taken together with the atoms to which they are bound, to form an unsubstituted fused heteroaryl or heterocyclyl group having 8 to 10 ring atoms and having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 10 or R 10' is independently -R 10b , -V R 10c , -T R 10b , or -Vi-T R 10b , wherein:
  • Vi is -NR 11 -, -NR u -C(0)-, -NR U -C(S)-, -NR U -C(NR U )-, -NR u C(0)0-, - NR u C(0)NR u -, -NR u C(0)S-, -NR u C(S)0-, -NR U C(S)NR U -, -NR U C(S)S-, - NR u C(NR u )0-, -NR 11 CfNR 1 ⁇ NR 11 -, -NR u S(0) 2 -, -NR u S(0) 2 NR u -, -C(O)-, -C0 2 -, - C(0)NR u -, -C(0)NR u O-, -S0 2 -, or -S0 2 NR u -; each occurrence of R a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic,
  • Ti is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R U )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(0)-, -C(0)0-, - C(0)N(R u )-, -S(0) 2 N(R u )-, -OC(0)N(R u )-, -N(R u )C(0)-, -N(R u )S0 2 -, - N(R lla )C(0)0-, -N(R 10a )C(O)N(R 10a )-, -N(R 10a )S(O) 2 N(R 10a )-, -OC(O)-, or -C(0)N(R u )- O- or wherein Ti forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocyclyl
  • each occurrence of R 10b is independently hydrogen, halogen, -CN, -N0 2 , -N(R U ) 2 , -OR 10a , -SR 10a , -S(O) 2 R 10a , -C(O)R 10a , -C(O)OR 10a , -C(0)N(R u ) 2 ,
  • each occurrence of R 10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
  • R 10a and R 1 ⁇ 3 ⁇ 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 11 is independently hydrogen, -C(0)R l la , -C0 2 R l la , -C(0)N(R l la ) 2 ,
  • Ci_ 6 aliphatic 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R l la is independently hydrogen or an optionally substituted group selected from C e aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9 is independently hydrogen, -C(0)R 9a , -C0 2 R 9a , -C(0)N(R 9b ) 2 , -S0 2 R 9a , - S0 2 N(R 9b ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9b is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R % taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3- to 6-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and provided that when HY is a non- fused group then HY is substituted with at least one occurrence of R 10 or R 10' , wherein R 10 or R 10' is:
  • Vi is -NR 11 -
  • Ti is a Ci -C3 alkylene chain
  • R is an optionally substituted 6- to 10-membered aryl ring or a 5- to 10-membered heteroaryl ring having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or Vi is -NR u C(0)NR u -
  • Ti is a Ci -C 3 alkylene chain
  • R 10b is -OR 10a ; or
  • Vi is -NR 11 -
  • R 10c is a 5- to 10-membered heteroaryl ring having 1 - 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 3 when R 3 is hydrogen, then R 1 is not , wherein R 103 is hydrogen or -C(0) 2 tBu; b) when R 3 is hydrogen, then R 1 is not -CH 2 OCH 2 CH 2 SiMe 3 ;
  • each occurrence of R 3 is independently hydrogen, -CN, halogen, -Z-R 5 , or an optionally substituted group selected from Ci_6 aliphatic and 3- to 10-membered cycloaliphatic, wherein:
  • Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
  • R 3a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 5 is hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10- membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each R 4 is independently selected from hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
  • R 4 is -Z 2 -R 6 wherein:
  • Z 2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-,
  • R 4a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 6 is hydrogen, -NH 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R , taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 2 is an optionally substituted group selected from 3- to 10-membered cycloaliphatic, 4- to 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R 2 is optionally substituted with 1 -4 occurrences of R 2a , wherein each occurrence of R 2a is independently -R 12a , -T 2 -R 12d , -T 2 -R 12a , or -V 2 -T 2 -R 12d , and:
  • each occurrence of R 12a is independently halogen, -CN, -N0 2 , -R 12c , -N(R 12b ) 2 ,
  • each occurrence of R 12b is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or two occurrences of R 12b , taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, Ci_6 haloaliphatic, 3- to 10-membered cycloaliphatic, 4- to 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12d is independently hydrogen or an optionally substituted group selected from 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 12e is independently hydrogen or an optionally substituted
  • Ci_6 aliphatic group each occurrence of V 2 is independently -N(R 12e )-, -0-, -S-, -S(0)-,
  • T 2 is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R 13 )-, -0-, -S-, -S(0)-, -S(0) 2 -, -C(O)-, -C(0)0-, -C(0)N(R 13 )-,
  • HY is a group selected from:
  • each occurrence of 3 ⁇ 4, X 5 , X 6 , X7, and X 8 is independently -CR 10 , -CR 10 , or N, provided no more than two occurrences of X4, X 5 , X 6 , X 7 , and X 8 is N;
  • each occurrence of Yi, Y 2 , Y 3 , Y 4 , Y 5 , Y 6 , Y 7 , and Y 8 is -CR 10 ;
  • each occurrence of Qi and Q 2 is independently S, O or -NR 9 ;
  • X4 and X 5 , Xe and X 7 , X 7 and Xg, Yi and -NR 9 , Y 3 and - NR 9 , or Y 4 and Y 5 may be taken together with the atoms to which they are bound, to form an unsubstituted fused heteroaryl or heterocyclyl group having 8 to 10 ring atoms and having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 10 or R 10' is independently -R 10b , -V R 10c , -T R 10b , or -Vi-T R 10b , wherein:
  • Vi is -NR 11 -, -NR u -C(0)-, -NR U -C(S)-, -NR U -C(NR U )-, -NR u C(0)0-, - NR u C(0)NR u -, -NR u C(0)S-, -NR u C(S)0-, -NR U C(S)NR U -, -NR U C(S)S-, - NR u C(NR u )0-, -NR 11 CfNR 1 ⁇ NR 11 -, -NR u S(0) 2 -, -NR u S(0) 2 NR u -, -C(O)-, -C0 2 -, - C(0)NR u -, -C(0)NR u O-, -S0 2 -, or -S0 2 NR u -; each occurrence of R a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic,
  • Ti is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R U )-, -0-, -S-, -S(O)-, -S(0) 2 -, -C(0)-, -C(0)0-, - C(0)N(R u )-, -S(0) 2 N(R u )-, -OC(0)N(R u )-, -N(R u )C(0)-, -N(R u )S0 2 -, - N(R lla )C(0)0-, -N(R 10a )C(O)N(R 10a )-, -N(R 10a )S(O) 2 N(R 10a )-, -OC(O)-, or -C(0)N(R u )- O- or wherein Ti forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocyclyl
  • each occurrence of R 10b is independently hydrogen, halogen, -CN, -N0 2 , -N(R U ) 2 , -OR 10a , -SR 10a , -S(O) 2 R 10a , -C(O)R 10a , -C(O)OR 10a , -C(0)N(R u ) 2 ,
  • each occurrence of R 10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
  • R 10a and R 1 ⁇ 3 ⁇ 4 taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 11 is independently hydrogen, -C(0)R l la , -C0 2 R l la , -C(0)N(R l la ) 2 ,
  • Ci_ 6 aliphatic 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R l la is independently hydrogen or an optionally substituted group selected from C e aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9 is independently hydrogen, -C(0)R 9a , -C0 2 R 9a , -C(0)N(R 9b ) 2 , -S0 2 R 9a , - S0 2 N(R 9b ) 2 , or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 9b is independently hydrogen or an optionally substituted group selected from Ci_ 6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R % taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3- to 6-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and provided that when HY is a non- fused group then HY is substituted with at least one occurrence of R 10 or R 10' , wherein R 10 or R 10' is:
  • Vi is -NR 11 -
  • Ti is a Ci -C3 alkylene chain
  • R is an optionally substituted 6- to 10-membered aryl ring or a 5- to 10-membered heteroaryl ring having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or Vi is -NR u C(0)NR u -
  • Ti is a Ci -C 3 alkylene chain
  • R 10b is -OR 10a ; or
  • Vi is -NR 11 -
  • R 10c is a 5- to 10-membered heteroaryl ring having 1 - 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 3 when R 3 is hydrogen, then R 1 is not , wherein R 103 is hydrogen or -C(0) 2 tBu; b) when R 3 is hydrogen, then R 1 is not -CH 2 OCH 2 CH 2 SiMe 3 ;
  • compounds of the invention may be optionally substituted with one or more substituents, such as are illustrated generally above, or as exemplified by particular classes, subclasses, and species of the invention.
  • substituents such as are illustrated generally above, or as exemplified by particular classes, subclasses, and species of the invention.
  • phrase “optionally substituted” is used interchangeably with the phrase “substituted or unsubstituted.”
  • substituted whether preceded by the term “optionally” or not, means that a hydrogen radical of the designated moiety is replaced with the radical of a specified substituent, provided that the substitution results in a stable or chemically feasible compound.
  • substituted when used in reference to a designated atom, means that attached to the atom is a hydrogen radical, which hydrogen atom can be replaced with the radical of a suitable substituent.
  • an "optionally substituted” group may have a substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position. Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds.
  • a stable compound or chemically feasible compound is one in which the chemical structure is not substantially altered when kept at a temperature from about -80°C to about +40°, in the absence of moisture or other chemically reactive conditions, for at least a week, or a compound which maintains its integrity long enough to be useful for therapeutic or prophylactic administration to a patient.
  • substituents refers to a number of substituents that equals from one to the maximum number of substituents possible based on the number of available bonding sites, provided that the above conditions of stability and chemical feasibility are met.
  • the term "independently selected” means that the same or different values may be selected for multiple instances of a given variable in a single compound.
  • a 3-7-membered saturated, partially unsaturated, or aromatic monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-10- membered partially unsaturated, or aromatic bicyclic ring system having 0-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur includes cycloaliphatic, heterocyclic, aryl and heteroaryl rings.
  • aromatic includes aryl and heteroaryl groups as described generally below and herein.
  • aliphatic or "aliphatic group”, as used herein, means an optionally substituted straight-chain or branched Ci_n hydrocarbon, or a cyclic hydrocarbon which is completely saturated or which contains one or more units of unsaturation, but which is not aromatic (also referred to herein as “carbocycle”, “cycloaliphatic”, “cycloalkyl”, or “cycloalkenyl”).
  • suitable aliphatic groups include optionally substituted linear, branched or cyclic alkyl, alkenyl, alkynyl groups and hybrids thereof, such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl, or (cycloalkyl)alkenyl.
  • aliphatic groups have 1-12, 1-10, 1-8, 1-6, 1-4, 1-3, or 1-2 carbon atoms.
  • alkyl used alone or as part of a larger moiety, refers to an optionally substituted straight or branched chain hydrocarbon group having 1-12, 1-10, 1-8, 1-6, 1-4, 1-3, or 1-2 carbon atoms.
  • alkenyl used alone or as part of a larger moiety, refers to an optionally substituted straight or branched chain hydrocarbon group having at least one double bond and having 2- 12, 2-10, 2-8, 2-6, 2-4, or 2-3 carbon atoms.
  • alkynyl used alone or as part of a larger moiety, refers to an optionally substituted straight or branched chain hydrocarbon group having at least one triple bond and having 2-12, 2-10, 2-8, 2-6, 2-4, or 2-3 carbon atoms.
  • cycloaliphatic refers to an optionally substituted saturated or partially unsaturated cyclic aliphatic ring system having from 3 to about 14 ring carbon atoms.
  • the cycloaliphatic group is an optionally substituted monocyclic hydrocarbon having 3-8 or 3-6 ring carbon atoms.
  • Cycloaliphatic groups include, without limitation, optionally substituted cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptyl, cycloheptenyl, cyclooctyl, cyclooctenyl, or cyclooctadienyl.
  • cycloaliphatic also include optionally substituted bridged or fused bicyclic rings having 6-12, 6- 10, or 6-8 ring carbon atoms, wherein any individual ring in the bicyclic system has 3-8 ring carbon atoms.
  • cycloalkyl refers to an optionally substituted saturated ring system of about 3 to about 10 ring carbon atoms.
  • exemplary monocyclic cycloalkyl rings include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cycloheptyl.
  • cycloalkenyl refers to an optionally substituted non-aromatic monocyclic or multicyclic ring system containing at least one carbon-carbon double bond and having about 3 to about 10 carbon atoms.
  • exemplary monocyclic cycloalkenyl rings include cyclopentyl, cyclohexenyl, and cycloheptenyl.
  • haloaliphatic refers to an aliphatic, alkyl, alkenyl or alkoxy group, as the case may be, which is substituted with one or more halogen atoms.
  • halogen or halo means F, CI, Br, or I.
  • fluoroaliphatic refers to a haloaliphatic wherein the halogen is fluoro, including perfluorinated aliphatic groups.
  • fluoroaliphatic groups include, without limitation, fluoromethyl, difluoromethyl, trifluoromethyl, 2- fluoroethyl, 2,2,2-trifluoroethyl, 1 , 1 ,2-trifluoroethyl, 1 ,2,2-trifluoroethyl, and pentafluoroethyl.
  • heteroatom refers to one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3,4-dihydro- 2H-pyrrolyl), NH (as in pyrrolidinyl) or NR + (as in N-substituted pyrrolidinyl)).
  • aryl and “ar-”, used alone or as part of a larger moiety e.g., "aralkyl”, “aralkoxy”, or “aryloxyalkyl” refer to an optionally substituted C6-i 4 aromatic hydrocarbon moiety comprising one to three aromatic rings.
  • the aryl group is a C6-ioaryl group.
  • Aryl groups include, without limitation, optionally substituted phenyl, naphthyl, or anthracenyl.
  • aryl and “ar-”, as used herein, also include groups in which an aryl ring is fused to one or more cycloaliphatic rings to form an optionally substituted cyclic structure such as a tetrahydronaphthyl, indenyl, or indanyl ring.
  • aryl may be used interchangeably with the terms “aryl group”, “aryl ring”, and “aromatic ring”.
  • an "aralkyl” or “arylalkyl” group comprises an aryl group covalently attached to an alkyl group, either of which independently is optionally substituted.
  • the aralkyl group is C6-io arylCi_ 6 alkyl, including, without limitation, benzyl, phenethyl, and naphthylmethyl.
  • heteroaryl and “heteroar-”, used alone or as part of a larger moiety refer to groups having 5 to 14 ring atoms, preferably 5, 6, 9, or 10 ring atoms; having 6, 10, or 14 ⁇ electrons shared in a cyclic array; and having, in addition to carbon atoms, from one to five heteroatoms.
  • a heteroaryl group may be mono-, bi-, tri-, or polycyclic, preferably mono-, bi-, or tricyclic, more preferably mono- or bicyclic.
  • heteroatom refers to nitrogen, oxygen, or sulfur, and includes any oxidized form of nitrogen or sulfur, and any quaternized form of a basic nitrogen.
  • a nitrogen atom of a heteroaryl may be a basic nitrogen atom and may also be optionally oxidized to the corresponding N-oxide.
  • heteroaryl When a heteroaryl is substituted by a hydroxy group, it also includes its corresponding tautomer.
  • heteroaryl and “heteroar-”, as used herein, also include groups in which a heteroaromatic ring is fused to one or more aryl, cycloaliphatic, or heterocycloaliphatic rings.
  • heteroaryl groups include thienyl, furanyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolizinyl, purinyl, naphthyridinyl, pteridinyl, indolyl, isoindolyl, benzothienyl, benzofuranyl, dibenzofuranyl, indazolyl, benzimidazolyl, benzthiazolyl, quinolyl, isoquinolyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, 4H-quinolizinyl
  • heteroaryl may be used interchangeably with the terms “heteroaryl ring”, “heteroaryl group”, or “heteroaromatic”, any of which terms include rings that are optionally substituted.
  • heteroarylkyl refers to an alkyl group substituted by a heteroaryl, wherein the alkyl and heteroaryl portions independently are optionally substituted.
  • heterocycle As used herein, the terms “heterocycle”, “heterocyclyl”, “heterocyclic radical”, and “heterocyclic ring” are used interchangeably and refer to a stable 3- to 8-membered monocyclic or 7-10- membered bicyclic heterocyclic moiety that is either saturated or partially unsaturated, and having, in addition to carbon atoms, one or more, preferably one to four, heteroatoms, as defined above.
  • nitrogen includes a substituted nitrogen.
  • the nitrogen may be N (as in 3,4-dihydro-2H-pyrrolyl), NH (as in pyrrolidinyl), or NR + (as in N-substituted pyrrolidinyl).
  • a heterocyclic ring can be attached to its pendant group at any heteroatom or carbon atom that results in a stable structure and any of the ring atoms can be optionally substituted.
  • saturated or partially unsaturated heterocyclic radicals include, without limitation, tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, decahydroquinolinyl, oxazolidinyl, piperazinyl, dioxanyl, dioxolanyl, diazepinyl, oxazepinyl, thiazepinyl, morpholinyl, and thiamorpholinyl.
  • a heterocyclyl group may be mono-, bi-, tri-, or polycyclic, preferably mono-, bi-, or tricyclic, more preferably mono- or bicyclic.
  • heterocyclylalkyl refers to an alkyl group substituted by a heterocyclyl, wherein the alkyl and heterocyclyl portions independently are optionally substituted.
  • a heterocyclic ring also includes groups in which the heterocyclic ring is fused to one or more aryl rings.
  • partially unsaturated refers to a ring moiety that includes at least one double or triple bond between ring atoms.
  • the term “partially unsaturated” is intended to encompass rings having multiple sites of unsaturation, but is not intended to include aromatic (e.g., aryl or heteroaryl) moieties, as herein defined.
  • alkylene refers to a bivalent alkyl group.
  • An "alkylene chain” is a polymethylene group, i.e., -(CH 2 ) n -, wherein n is a positive integer, preferably from 1 to 6, from 1 to 4, from 1 to 3, from 1 to 2, or from 2 to 3.
  • An optionally substituted alkylene chain is a polymethylene group in which one or more methylene hydrogen atoms is optionally replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group and also include those described in the specification herein. It will be appreciated that two substituents of the alkylene group may be taken together to form a ring system.
  • two substituents can be taken together to form a 3-7-membered ring.
  • the substituents can be on the same or different atoms.
  • An alkylene chain also can be optionally interrupted by a functional group.
  • An alkylene chain is "interrupted" by a functional group when an internal methylene unit is interrupted by the functional group. Examples of suitable "interrupting functional groups" are described in the specification and claims herein.
  • An aryl (including aralkyl, aralkoxy, aryloxyalkyl and the like) or heteroaryl (including heteroaralkyl and heteroarylalkoxy and the like) group may contain one or more substituents and thus may be "optionally substituted".
  • An aliphatic or heteroaliphatic group, or a non-aromatic carbocyclic or heterocyclic ring may contain one or more substituents and thus may be "optionally substituted".
  • a ring nitrogen atom of a heteroaryl or non-aromatic heterocyclic ring also may be oxidized to form the corresponding N-hydroxy or N-oxide compound.
  • a nonlimiting example of such a heteroaryl having an oxidized ring nitrogen atom is N-oxidopyridyl.
  • two independent occurrences of R + are taken together with their intervening atom(s) to form a monocyclic or bicyclic ring selected from 3-13-membered cycloaliphatic, 3-12-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • Exemplary rings that are formed when two independent occurrences of R + (or any other variable similarly defined in the specification and claims herein), are taken together with their intervening atom(s) include, but are not limited to the following: a) two independent occurrences of R + (or any other variable similarly defined in the specification or claims herein) that are bound to the same atom and are taken together with that atom to form a ring, for example, N(R + ) 2 , where both occurrences of R + are taken together with the nitrogen atom to form a piperidin- 1 -yl, piperazin- 1 -yl, or morpholin-4-yl group; and b) two independent occurrences of R + (or any other variable similarly defined in the specification or claims herein) that are bound to different atoms and are taken together with both of those atoms to form a ring,
  • Exemplary rings that are formed when two independent occurrences of 3 ⁇ 4 and X 5 , Xe and X 7 , or X 7 and X 8 ; are taken together with their intervening atom(s) to form a fused group having 8 to 10 ring atoms include ited to the following:
  • Exemplary rings that are formed when two independent occurrences of Yi and -NR 9 , Y 3 and -NR 9 , Y 4 and Y 5 , or Y 6 and Y 7 are taken together with their intervening atom(s) to form a fused group having 8 to 10 rin atoms include, but are not limited to the following:
  • structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, and geometric (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, (Z) and (E) double bond isomers, and (Z) and (E) conformational isomers. Therefore, single stereochemical isomers as well as enantiomeric, diastereomeric, and geometric (or conformational) mixtures of the present compounds are within the scope of the invention. Unless otherwise stated, all tautomeric forms of the compounds of the invention are within the scope of the invention.
  • structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms.
  • compounds having the present structures where there is a replacement of hydrogen by deuterium or tritium, or a replacement of a carbon by a 13 C- or 14 C-enriched carbon are within the scope of this invention.
  • Such compounds are useful, as a nonlimiting example, as analytical tools or probes in biological assays.
  • the present invention encompasses one enantiomer of inhibitor free from the corresponding optical isomer, racemic mixture of the inhibitor and mixtures enriched in one enantiomer relative to its corresponding optical isomer.
  • the mixture contains, for example, an enantiomeric excess of at least 50%, 75%, 90%, 95% 99% or 99.5%.
  • the enantiomers of the present invention may be resolved by methods known to those skilled in the art, for example by formation of diastereoisomenc salts which may be separated, for example, by crystallization; formation of diastereoisomeric derivatives or complexes which may be separated, for example, by crystallization, gas-liquid or liquid chromatography; selective reaction of one enantiomer with an enantiomer-specific reagent, for example enzymatic esterification; or gas-liquid or liquid chromatography in a chiral environment, for example on a chiral support for example silica with a bound chiral ligand or in the presence of a chiral solvent.
  • enantiomers may be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents, or by converting one enantiomer into the other by asymmetric transformation.
  • the present invention encompasses a diastereomer free of other diastereomers, a pair of diastereomers free from other diasteromeric pairs, mixtures of diasteromers, mixtures of diasteromeric pairs, mixtures of diasteromers in which one diastereomer is enriched relative to the other diastereomer(s) and mixtures of diasteromeric pairs in which one diastereomeric pair is enriched relative to the other diastereomeric pair(s).
  • the mixture is enriched in one diastereomer or diastereomeric pair(s) relative to the other diastereomers or diastereomeric pair(s), the mixture is enriched with the depicted or referenced diastereomer or diastereomeric pair(s) relative to other diastereomers or diastereomeric pair(s) for the compound, for example, by a molar excess of at least 50%, 75%, 90%, 95%, 99% or 99.5%.
  • the diastereoisomeric pairs may be separated by methods known to those skilled in the art, for example chromatography or crystallization and the individual enantiomers within each pair may be separated as described above. Specific procedures for chromatographically separating diastereomeric pairs of precursors used in the preparation of compounds disclosed herein are provided the examples herein.
  • variables HY, R 1 , R 2 , and R 3 are as defined above for formula IA.
  • variables HY, R 1 , R 2 , and R 3 are as defined above for formula IA.
  • variables HY, R 1 , R 2 , and R 3 are as defined above for formula IA.
  • R 1 is
  • CY and CY is 1 or 11 ; wherein:
  • Xi, X 2 , and X3 are each independently N, O, S, NR 4 , or CR 7 , provided that only one of
  • Xi, X2, or X 3 may be O or S;
  • Y 9 is nitrogen or carbon
  • R 4 is independently hydrogen, -Z 2 -R 6 , optionally substituted
  • Ci_6 aliphatic or optionally substituted 3-10-membered cycloaliphatic, wherein:
  • Z 2 is selected from an optionally substituted Ci_ 3 alkylene chain, -S(O)-, -S(0) 2 -, -C(O)-, -C0 2 -, -C(0)NR 4a -, or -S(0) 2 NR 4 ⁇
  • R 4a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 6 is an optionally substituted group selected from d_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 7 and R 7 is independently hydrogen, -CN, halogen, -Z 3 -R 8 , Ci_6 aliphatic, or 3-10-membered cycloaliphatic, wherein:
  • Z 3 is selected from an optionally substituted Ci_ 3 alkylene chain, -0-, -N(R 7a )-, - S-, -S(O)-, -S(0) 2 -, -C(O)-, -C0 2 -, -C(0)NR 7 ⁇ -N(R 7a )C(0)-, -N(R 7a )C0 2 -, -S(0) 2 NR 7a -, -N(R 7a )S(0) 2 -, -OC(0)N(R 7a )-, -N(R 7a )C(0)NR 7a -, -N(R 7a )S(0) 2 N(R 7a )-, or -OC(O)-;
  • R 7a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 8 is an optionally substituted group selected from d_6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • R 1 is
  • CY and CY is 1 or 11 ; wherein: Xi, X 2 , and X3, are each independently N, O, S, NR 4 , or CR 7 , provided that only one of
  • Xi, X2, or X 3 may be O or S;
  • Y 9 is nitrogen or carbon
  • R 4 is independently hydrogen, -Z 2 -R 6 , optionally substituted
  • Ci_6 aliphatic or optionally substituted 3- 10-membered cycloaliphatic, wherein:
  • Z 2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-, -S(0) 2 -, -C(O)-, -CO 2 -, -C(0)NR 4a -, or -S(0) 2 NR 4a -,
  • R 4a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 6 is an optionally substituted group selected from d_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • each occurrence of R 7 and R 7 is independently hydrogen, -CN, halogen, -NH 2 , -Z 3 -R 8 , Ci_6 aliphatic, or 3-10-membered cycloaliphatic, wherein:
  • Z 3 is selected from an optionally substituted Q_3 alkylene chain, -0-, -N(R 7a )-, - S-, -S(O)-, -S(0) 2 -, -C(O)-, -CO 2 -, -C(0)NR 7a -, -N(R 7a )C(0)-, -N(R 7a )C0 2 -, -S(0) 2 NR 7a -, -N(R 7a )S(0) 2 -, -OC(0)N(R 7a )-, -N(R 7a )C(0)NR 7a -, -N(R 7a )S(0) 2 N(R 7a )-, or -OC(O)-;
  • R 7a is hydrogen or an optionally substituted Ci_4 aliphatic
  • R 8 is hydrogen or an optionally substituted group selected from d_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • CY is 1
  • Y 9 is carbon
  • Xi is nitrogen
  • G 14 is N(R 4 )
  • X 2 and X3, are CH.
  • Y9 is carbon
  • Xi and X3 are nitrogen
  • G 14 is N(R 4 )
  • X 2 is CH.
  • Y 9 is carbon
  • Xi and G 14 are nitrogen
  • X 3 is N(R 4 )
  • X 2 is CH.
  • Y 9 is carbon, Xi and X 2 are nitrogen, G14 is N(R 4 ), and X3 is CH.
  • Y 9 is carbon, G14 is N(R 4 ), X 3 is nitrogen, and Xi and X 2 are CH. [0070] In other embodiments, Y 9 is carbon, G 14 is nitrogen, X 3 is N(R 4 ), and Xi and X 2 are CH.
  • Y 9 is carbon
  • X 3 is nitrogen
  • X 2 is N(R 4 )
  • Xi and G 14 are CH.
  • Y 9 is carbon
  • X 2 is nitrogen
  • G 14 is N(R 4 )
  • Xi and X3, are CH.
  • Y 9 is carbon
  • X 2 is N(R 4 )
  • G 14 is nitrogen
  • Xi and X3, are CH.
  • R is Cy and Cy is an optionally substituted 6-membered aryl or heteroaryl ring.
  • R 1 is Cy and Cy is an optionally substituted 5- to 6-membered heteroaryl or heterocyclyl ring.
  • R 1 is Cy and Cy is selected from:
  • R 1 is optionally further substituted with one or more occurrences of R 7 or R 4' .
  • R 1 is Cy
  • Cy is selected from:
  • Cy is optionally further substituted with one or more occurrences ofR 7 or R 4' .
  • R 1 is Cy, and Cy is selected from:
  • R 1 is optionally further substituted with one or more occurrences of R 7 or R 4' .
  • R 1 is Cy
  • Cy is an optionally substituted 6-membered aryl ring.
  • R 1 is -CON(R 4 ) 2 , -C(0)OR 4 , -NHCOR 4 , or CH 2 OR 4 .
  • R 7 , R 4' , and R 14 are as defined in any one of the embodiments described herein.
  • HY is selected from:
  • HY is selected from:
  • each occurrence of X 5 , ⁇ , and X 7 is independently -CR 10 , -CR 10 or N, provided no more than two occurrences of X 5 , ⁇ , and X 7 are N;
  • each occurrence of Qi and Q 2 is independently S, O or -NR 9 ;
  • each occurrence of Yi and Y 7 is independently -CR 10 ;
  • HY is selected from:
  • HY is selected from:
  • HY is selected from:
  • each non- fused HY group is substituted with one or more occurrences of R 10 or R 10 , and at least one occurrence of R 10 or R 10' is -N(R u )C(O)R 10a , -N(R u )C(O)OR 10a , or -C(0)N(R u ) 2 , and the dashed line represents a single bond or a double bond.
  • R 10a is Ci_6 aliphatic substituted with a 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • HY is selected from:
  • each fused HY group is unsubstituted, and each non- fused HY group is substituted with one or more occurrences of R 10 or R 10 , and at least one occurrence of R 10 or R 10 is -N(R u )C(O)R 10a , - N(R u )C(O)OR 10a , or -C(0)N(R u ) 2 , and the dashed line represents a single bond or a double bond.
  • HY is , wherein HY is substituted with one or more occurrences of R 10 or R 10 .
  • HY is , wherein R 10 is hydrogen, methyl, chloro, bromo, fluoro, CN, CF 3 , OR 10a , COR 10a , and R 10 is NHCOR 10a or -NHC(O)OR 10a .
  • R 10 is hydrogen, methyl, or chloro, and R 10 is -NHCOR 10a or -NHCOOR 10a .
  • R 10 is hydrogen, methyl, or chloro
  • R 10 is -NHR 11 , wherein R 11 is an optionally substituted group selected from 6- 10-membered aryl, or 5-10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • R 10 is hydrogen, methyl, or chloro.
  • R 10' is methyl
  • R 10 is -NHCOR 10a
  • R 10 is -NHR 11 , wherein R 11 is an optionally substituted 5- to 10- membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • R 10a is cyclopropyl, methyl, ethyl, or isopropyl.
  • R 1 , R 2 , R 3 , and R 14 are as defined in any one of the embodiments described herein.
  • R 2 is a 6- 10- membered aryl or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; optionally substituted with 1 -3 occurrences of R 2a .
  • R 2 is a phenyl or pyridyl group
  • R 2 is a phenyl group; optionally substituted with 1 to 4 independent occurrences of halogen, Ci_ 3 alkyl, -CN, Ci_ 3 haloalkyl, -(CH 2 ) p N(R 12b ) 2 , -OR 12b , -NHC(0)R 12b , - NHC(0)NHR 12b , -NHS(0) 2 R 12b , -S(0) 2 R 12c , -S(0) 2 N(R 12b ) 2 , -C(0)OR 12b , -C(0)N(R 12b ) 2 , or -C(0)R 12b ; wherein R 12b and R 12c are defined as described herein or
  • R 12b taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0-1 additional heteroatoms selected from nitrogen, oxygen, or sulfur, and wherein p is 0 to 3.
  • R 2 is a phenyl group; optionally substituted with 1 to 4 independent occurrences of halogen, Ci_ 3 alkyl, -CN, Ci_ 3 haloalkyl, -(CH 2 ) p N(R 12b ) 2 , -OR 12b , -NHC(0)R 12b , - NHC(0)NHR 12b , -NHS(0) 2 R 12b , -S(0) 2 R 12c , -S(0) 2 N(R 12b ) 2 , C(0)OR 12b , -C(0)N(R 12b ) 2 , or -C(0)R 12b , and wherein p is 0 to 3.
  • R 2 is a phenyl group; optionally substituted with 1 to 4 independent occurrences of halogen, Ci_ 3 alkyl, -CN, Ci_ 3 haloalkyl, -CH 2 N(CH 3 ) 2 , -OCi_ 3 alkyl, -OCi_ 3 haloalkyl, -SCi_ 3 haloalkyl, -NHC(0)Ci_ 3 alkyl, -NHC(0)NHCi_ 3 alkyl, -NHS(0) 2 Ci_ 3 alkyl, or -C(0)H.
  • R 2 is substituted with 1 or 2 independent occurrences of R 2a .
  • R 2a is a halo or methyl group.
  • R 2 is a phenyl group substituted with 1 or 2 occurrences of halogen. In yet other embodiments, R 2 is a phenyl group substituted with methyl.
  • R 2 is a 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • R 2 is an optionally substituted N-linked 3-, 4-, 5-, 6-, or 7- membered heterocyclyl ring, optionally substituted with one or more occurrences of R 2a .
  • R 2 is optionally substituted with one or more Q_ 3 alkyl groups, - [00109] In still other embodiments, R 2 is a Ci_6 aliphatic and each occurrence of R 2a is independently -
  • R 2 is a Ci_6 aliphatic, optionally substituted with halo, -N(R 12b ) 2 , or a cyclopropyl ring, wherein each R 12b is independently selected from hydrogen, methyl, or ethyl, or wherein two R 12b , taken together with a nitrogen atom to which they are bound, form a pyrrolidinyl ring.
  • R 2 is a d_3 aliphatic.
  • R 2 is halogen. In other embodiments, R 2 is hydrogen.
  • R 1 is CY, -CON(R 4 ) 2 , -NHCOR 4 , or -COOR 4 ;
  • R 2 is an optionally substituted 6- 10-membered aryl or 5-10- membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and
  • HY is selected from
  • each non-fused HY group is substituted with one or more occurrences of R 10 or R 10 , and at least one occurrence of R 10 or R 10' is -N(R u )C(O)R 10a or -C(0)N(R u ) 2 , and the dashed line represents a single bond or a double bond.
  • Examples of the solvent for the below-mentioned reactions include, but are not limited to halogenated hydrocarbons such as dichloromethane, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like, aromatic hydrocarbons such as benzene, toluene, xylene and the like, alcohols such as methanol, ethanol, isopropanol, fert-butanol, phenol and the like, ethers such as diethyl ether, tetrahydrofuran, dioxane, DME and the like, acetone, ACN, ethyl acetate, NN-dimethylformamide, NN- dimethylacetamide, 1 -methyl-2-pyrrolidone, dimethyl sulfoxide, hexamethylphosphoramide, water or a mixed solvent thereof and the like.
  • halogenated hydrocarbons such as dichloromethane, chloroform, carbon tetrachlor
  • reaction conditions including variations in solvent, reagents, catalysts, reaction temperatures and times are possible for each of the reactions described. Variation of order of synthetic steps and alternative synthetic routes are also possible.
  • synthesis can be started from commercially available imidazole analogs to prepare target compounds.
  • specially functionalized imidazole analogs can be prepared by the procedures described in the Schemes below.
  • Scheme 1 describes a method of preparing substituted imidazoles xv.
  • Treatment of methyl lH-imidazole-5-carboxylate (viii) with a brominating reagent such as NBS in an appropriate solvent gives ix. Protection of the imidazole nitrogen with a standard protecting group (PG) s u Ch as SEM gives compounds x.
  • Compounds xi can then be prepared from compounds x byod A can subsequently be used to prepare compounds xii from xi when R 2 is an aromatic or heteroaromatic group.
  • compounds xii can be prepared by Metho d B Co m po u nds xiv can be prepared via the intermediate acids xiii (obtained by hydrolysis of the ester of compounds xii under standard conditions) or by transformation of the esters xii directly to a variety of groups using standard methods.
  • Scheme 2 describes a method of preparing substituted imidazoles xix.
  • Compounds xviii can be prepared by treatment of compounds xvii according to Method A.
  • Fully substituted imidazoles xix can then be obtained by again using Method A.
  • Scheme 3 describes a method of preparing substituted imidazoles xxv. Protection of the imidazole (xx) nitrogen with a standard protecting group (PG) such as SEM gives compounds xxi.
  • PG standard protecting group
  • Compounds xxii can be prepared by treatment of compounds xxi according to Method A. Metal halogen exchange with a base such as «-BuLi in a solvent, for example THF, followed by quench with water can provide compounds xxiii from imidazoles xxii.
  • Compounds xxiv can be prepared by treatment of compounds xxiii according to Method A.
  • Fully substituted imidazoles xxv can be obtained from compounds xxiv by removal of the protecting group under standard conditions followed by treatment with an aryl fluoride in the presence of a base such as potassium carbonate in a solvent, for example DMF. Alternatively, following removal of the protecting group under standard conditions compounds xxv can be prepared according to Method B.
  • the compounds of the present invention can be prepared by methods known to one of ordinary skill in the art and / or by reference to the schemes shown below and the synthetic examples that follow.
  • the present invention provides compounds that are useful as inhibitors of VPS34 and/or PI3K, and thus the present compounds are useful for treating proliferative, inflammatory, or cardiovascular disorders such as tumor and/or cancerous cell growth mediated by VPS34 and/or PI3K.
  • the compounds are useful in the treatment of cancers in a subject, including, but not limited to, lung and bronchus, including non-small cell lung cancer (NSCLC), squamous lung cancer, brochioloalveolar carcinoma (BAC), adenocarcinoma of the lung, and small cell lung cancer (SCLC); prostate, including androgen-dependent and androgen-independent prostate cancer; breast, including metastatic breast cancer; pancreas; colon and rectum; thyroid; liver and intrahepatic bile duct; hepatocellular; gastric; endometrial; melanoma; kidney; and renal pelvis, urinary bladder; uterine corpus; uterine cervix; ovary, including progressive epithelial or primary peritoneal cancer; multiple myeloma; esophagus; acute myelogenous leukemia (AML); chronic myelogenous leukemia (CML), including accelerated CML and CML blast phase (CML-BP); lymph
  • compounds of the invention are suitable for the treatment of breast cancer, bladder cancer, colon cancer, glioma, glioblastoma, lung cancer, hepatocellular cancer, gastric cancer, melanoma, thyroid cancer, endometrial cancer, renal cancer, cervical cancer, pancreatic cancer, esophageal cancer, prostate cancer, brain cancer, or ovarian cancer.
  • compounds of the invention are suitable for the treatment of inflammatory and cardiovascular disorders including, but not limited to, allergies/anaphylaxis, acute and chronic inflammation, rheumatoid arthritis; autoimmunity disorders, thrombosis, hypertension, cardiac hypertrophy, and heart failure.
  • compositions comprising any of the compounds as described herein, and optionally comprise a pharmaceutically acceptable carrier, adjuvant or vehicle.
  • these compositions optionally further comprise one or more additional therapeutic agents.
  • certain of the compounds of present invention can exist in free form for treatment, or where appropriate, as a pharmaceutically acceptable derivative thereof.
  • a pharmaceutically acceptable derivative includes, but is not limited to, pharmaceutically acceptable prodrugs, salts, esters, salts of such esters, or any other adduct or derivative which upon administration to a patient in need is capable of providing, directly or indirectly, a compound as otherwise described herein, or a metabolite or residue thereof.
  • the term “pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio.
  • a “pharmaceutically acceptable salt” means any non-toxic salt or salt of an ester of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof.
  • the term “inhibitorily active metabolite or residue thereof means that a metabolite or residue thereof is also an inhibitor of VPS34 and/or PI3K.
  • compositions of this invention include those derived from suitable inorganic and organic acids and bases.
  • Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
  • inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid
  • organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
  • salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclop entanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pect
  • Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N + (Ci_ 4 alkyl) 4 salts.
  • This invention also envisions the quaternization of any basic nitrogen-containing groups of the compounds disclosed herein. Water or oil-soluble or dispersable products may be obtained by such quaternization.
  • Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like.
  • Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate.
  • the pharmaceutically acceptable compositions of the present invention additionally comprise a pharmaceutically acceptable carrier, adjuvant, or vehicle, which, as used herein, includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired.
  • a pharmaceutically acceptable carrier, adjuvant, or vehicle which, as used herein, includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired.
  • Remington's Pharmaceutical Sciences, Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa., 1980) discloses various carriers used in formulating pharmaceutically acceptable compositions
  • any conventional carrier medium is incompatible with the compounds of the invention, such as by producing any undesirable biological effect or otherwise interacting in a deleterious manner with any other component(s) of the pharmaceutically acceptable composition, its use is contemplated to be within the scope of this invention.
  • materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, or potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, wool fat, sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc
  • a method for treating a proliferative, inflammatory, or cardiovascular disorder comprising administering an effective amount of a compound, or a pharmaceutical composition to a subject in need thereof.
  • an "effective amount" of the compound or pharmaceutical composition is that amount effective for treating a proliferative, inflammatory, or cardiovascular disorder, or is that amount effective for treating cancer.
  • an "effective amount" of a compound is an amount which inhibits binding of PI3K and thereby blocks the resulting signaling cascades that lead to the abnormal activity of growth factors, receptor tyrosine kinases, protein serine/threonine kinases, G protein coupled receptors and phospholipid kinases and phosphatases.
  • the compounds and compositions, according to the method of the present invention may be administered using any amount and any route of administration effective for treating the disease.
  • the exact amount required will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the disorder, the particular agent, its mode of administration, and the like.
  • the compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage.
  • dosage unit form refers to a physically discrete unit of agent appropriate for the patient to be treated. It will be understood, however, that the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment.
  • the specific effective dose level for any particular patient or organism will depend upon a variety of factors including the disease being treated and the severity of the disease; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed, and like factors well known in the medical arts.
  • patient means an animal, preferably a mammal, and most preferably a human.
  • compositions of this invention can be administered to humans and other animals orally, rectally, parenterally, intracisternally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated.
  • the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.
  • Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs.
  • the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
  • the oral compositions can also include adj
  • Injectable preparations for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents.
  • the sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol.
  • the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S. P. and isotonic sodium chloride solution.
  • sterile, fixed oils are conventionally employed as a solvent or suspending medium.
  • any bland fixed oil can be employed including synthetic mono- or diglycerides.
  • fatty acids such as oleic acid are used in the preparation of injectables.
  • the injectable formulations can be sterilized, for example, by filtration through a bacterial- retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
  • a compound of the present invention In order to prolong the effect of a compound of the present invention, it is often desirable to slow the absorption of the compound from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then depends upon its rate of dissolution that, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered compound form is accomplished by dissolving or suspending the compound in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the compound in biodegradable polymers such as polylactide-polyglycolide.
  • the rate of compound release can be controlled.
  • biodegradable polymers include poly(orthoesters) and poly(anhydrides).
  • Depot injectable formulations are also prepared by entrapping the compound in liposomes or microemulsions that are compatible with body tissues.
  • compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
  • suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
  • Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
  • the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar— agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl
  • Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
  • the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.
  • the active compounds can also be in micro-encapsulated form with one or more excipients as noted above.
  • the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art.
  • the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch.
  • Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose.
  • the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner.
  • buffering agents include polymeric substances and waxes.
  • Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches.
  • the active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required.
  • Ophthalmic formulation, ear drops, and eye drops are also contemplated as being within the scope of this invention.
  • the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body.
  • Such dosage forms can be made by dissolving or dispensing the compound in the proper medium.
  • Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel.
  • inventive compounds may be used in an application of monotherapy to treat a disorder, disease or symptom, they also may be used in combination therapy, in which the use of an inventive compound or composition (therapeutic agent) is combined with the use of one or more other therapeutic agents for treating the same and/or other types of disorders, symptoms and diseases.
  • Combination therapy includes administration of the therapeutic agents concurrently or sequentially.
  • the therapeutic agents can be combined into one composition which is administered to the patient.
  • the compounds of this invention are used in combination with other therapeutic agents, such as other inhibitors of VPS34 and/or PI3K.
  • a compound of the invention is administered in conjunction with a therapeutic agent selected from the group consisting of cytotoxic agents, radiotherapy, and immunotherapy. It is understood that other combinations may be undertaken while remaining within the scope of the invention.
  • Another aspect of the invention relates to inhibiting VPS34 and /or PI3K, activity in a biological sample or a patient, which method comprises administering to the patient, or contacting said biological sample with a compound of formula IA, IIA, IIIA, or IVA, or a composition comprising said compound.
  • biological sample generally includes in vivo, in vitro, and ex vivo materials, and also includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
  • Still another aspect of this invention is to provide a kit comprising separate containers in a single package, wherein the inventive pharmaceutical compounds, compositions and/or salts thereof are used in combination with pharmaceutically acceptable carriers to treat disorders, symptoms and diseases where VPS34 and/or PI3K kinase plays a role.
  • Table 1 below depicts certain compounds represented by compounds of general formula IA, and subsets IIA, IIIA, IVA, or VA.
  • LCMS spectra were recorded on a Hewlett-Packard HP 1 100 or Agilent 1 100 Series LC system connected to a Micromass mass spectromteter using reverse phase CI 8 columns. Various gradients and run times were selected in order to best characterize the compounds. Mobile phases were based on ACN/water gradients and contained either 0.1% formic acid (methods indicated FA) or 10 mM ammonium acetate (methods indicated AA).
  • Step 2 A f -[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)pyridin-2-yl]acetamide
  • Step 1 methyl 2,4-dibromo-lH-imidazole-5-carboxylate
  • Step 2 methyl 2,4-dibromo-l- ⁇ [2-(trimethylsilyl)ethoxy]methyl ⁇ -lH-imidazole-5- carboxylate
  • Step 3 Methyl 2-(2-acetamidopyridin-4-yl)-4-bromo-l- ⁇ [2-(trimethylsilyl)- ethoxy]methyl ⁇ -lH-imidazole-5-carboxylate
  • Step 4 methyl 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l- ⁇ [2- (trimethylsilyl)ethoxy]methyl ⁇ -lH-imidazole-5-carboxylate
  • Step 5 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l- ⁇ [2-(trimethylsilyl)- ethoxy] methyl ⁇ -lH-imidazole-5-carboxylic acid
  • Step 6 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l- ⁇ [2-(trimethylsilyl)- ethoxy]methyl ⁇ -lH-imidazole-5-carboxamide
  • Step 7 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l- ⁇ [2-(trimethylsilyl)- ethoxy]methyl ⁇ -lH-imidazole-5-carboxamide
  • Step 1 methyl 2-(2-acetamido-5-bromopyridin-4-yl)-4-(2,4-dichlorophenyl)-lH- imidazole-5-carboxylate
  • Step 2 methyl 2-(2-acetamido-5-bromopyridin-4-yl)-4-(2,4-dichlorophenyl)-l-vinyl-lH- imidazole-5-carboxylate
  • Step 3 methyl 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2,l-a] [2,6]naphthyridine-3- carboxylate
  • Step 4 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2,l-a] [2,6]naphthyridine-3- carboxylic acid
  • Step 5 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2,l-a] [2,6]naphthyridine-3- carboxamide
  • Step 2 5-[4-bromo-l-(2,4-dichlorophenyl)-lH-imidazol-2-yl]-l- ⁇ [2-(trimethylsilyl)- ethoxy]methyl ⁇ -lH-pyrazole
  • Step 3 A ⁇ - ⁇ 4-[l-(2,4-dichlorophenyl)-2-(l- ⁇ [2-(trimethylsilyl)ethoxy]methyl ⁇ -lH- pyrazol-5-yl)-lH-imidazol-4-yl]-5-methylpyridin-2-yl ⁇ acetamide
  • Step 4 A ⁇ - ⁇ 4-[l-(2,4-dichlorophenyl)-2-(lH-pyrazol-5-yl)-lH-imidazol-4-yl]-5- methylpy ridin-2-yl ⁇ acetamide
  • Example 5 ⁇ - ⁇ 4-[2-(2- ⁇ 1 ⁇ 1 ⁇ 1 ⁇ 1)-1-( ⁇ -2- ⁇ 1)-1 ⁇ 3 ⁇ 1-4- ⁇ 1] ⁇ -2- yl ⁇ acetamide (1-105)
  • Step 1 2,4,5-tribromo-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-imidazole

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Abstract

This invention provides compounds of formula IA: and also provides compounds of formula IIA, IIIA, IVA, or VA: wherein HY, R1, R2, R3, R14, G1, G2, G3 and G4 are as described in the specification. The compounds are inhibitors of VPS34 and/or PI3K and are thus useful for treating proliferative, inflammatory, or cardiovascular disorders.

Description

HETEROARYLS AND USES THEREOF
BACKGROUND OF THE INVENTION
[0001] This application claims priority from U.S. Provisional Patent Application Ser. No. 61/579,711, filed on December 23, 201 1, U.S. Provisional Patent Application Ser. No. 61/672,030, filed on July 16, 2012, and U.S. Provisional Patent Application Ser. No. 61/716, 172, filed on October 19, 2012.
[0002] Phosphatidylinositol 3-kinase (PI3K) is a family of lipid kinases that phosphorylate phosphatidylinositol at the 3' position of the inositol ring. PI3K is comprised of several classes of genes, including Class IA, IB, II and III and some of these classes contain several isoforms (reviewed in Engelman et al., Nature Review Genetics 7:606-619 (2006)). Adding to the complexity of this family is the fact that PI3Ks function as heterodimers, comprising a catalytic domain and a regulatory domain. The PI3K family is structurally related to a larger group of lipid and serine/threonine protein kinases known as the phosphatidylinositol 3-kinase like kinases (PIKKs), which also includes DNA-PK, ATM, ATR, mTOR, TRRAP and SMG1.
[0003] PI3K is activated downstream of various mitogenic signals mediated through receptor tyrosine kinases, and subsequently stimulates a variety of biological outcomes; including increased cell survival, cell cycle progression, cell growth, cell metabolism, cell migration and angiogenesis (reviewed in Cantley, Science 296: 1655-57 (2002); Hennessy et al., Nature Reviews Drug Discovery 4:988-1004 (2005); Engelman et al., Nature Review Genetics 7:606-619 (2006)). Thus, PI3K hyper-activation is associated with a number of hyper-proliferative, inflammatory, or cardiovascular disorders; including cancer, inflammation, and cardiovascular disease.
[0004] There are a number of genetic aberrations that lead to constitutive PI3K signaling; including activating mutations in PI3K itself (Hennessy et al., Nature Reviews Drug Discovery 4:988-1004 (2005); reviewed in Bader et al., Nature Reviews Cancer 5:921-9 (2005)); RAS (reviewed in Downward Nature Reviews Cancer 3: 1 1-22 (2003)) and upstream receptor tyrosine kinases (reviewed in Zwick et al., Trends in Molecular Medicine 8: 17-23 (2002)) as well as inactivating mutations in the tumor suppressor PTEN (reviewed in Cully et al., Nature Reviews Cancer 6: 184-92 (2006)). Mutations in each of these gene classes have proven to be oncogenic and are commonly found in a variety of cancers.
[0005] The molecules defined within this invention inhibit the activity of PI3K, and therefore may be useful for the treatment of proliferative, inflammatory, or cardiovascular disorders. Cases where PI3K pathway mutations have been linked to proliferative disorders where the molecules defined within this invention may have a therapeutic benefit include benign and malignant tumors and cancers from diverse lineage, including but not limited to those derived from colon (Samuels et al., Science 304:554 (2004); reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006)), liver (reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006)), intestine (reviewed in Hennessy et al., Nature Reviews Drug Discovery 4:988- 1004 (2005)), stomach (Samuels et al., Science 304:554 (2004); reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006)), esophagus (Phillips et al., International Journal of Cancer 1 18:2644-6 (2006)); pancreas (reviewed in Downward Nature Reviews Cancer 3 : 1 1-22 (2003)); skin (reviewed in Hennessy et al., Nature Reviews Drug Discovery 4:988- 1004 (2005)), prostate (reviewed in Hennessy et al., Nature Reviews Drug Discovery 4:988- 1004 (2005)), lung (Samuels et al., Science 304:554 (2004); reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006)), breast (Samuels et al., Science 304:554 (2004); Isakoff et al., Can Res 65: 10992- 1000 (2005); reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006)), endometrium (Oda et al., Can Res 65: 10669- 73 (2005); reviewed in Hennessy et al., Nature Reviews Drug Discovery 4:988- 1004 (2005)), cervix (reviewed in Hennessy et al., Nature Reviews Drug Discovery 4:988- 1004 (2005)); ovary (Shayesteh et al., Nature Genetics 21 :99- 102 (1999); reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006)), testes (Moul et al., Genes Chromosomes Cancer 5: 109- 18 (1992); Di Vizio et al., Oncogene 24: 1882-94 (2005)), hematological cells (reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006); Hennessy et al., Nature Reviews Drug Discovery 4:988- 1004 (2005)), pancreas (reviewed in Downward Nature Reviews Cancer 3 : 1 1-22 (2003)), thyroid (reviewed in Downward Nature Reviews Cancer 3 : 1 1 -22 (2003); reviewed in Hennessy et al., Nature Reviews Drug Discovery 4:988- 1004 (2005)); brain (Samuels et al., Science 304:554 (2004); reviewed in Karakas et al., British Journal of Cancer 94: 455-59 (2006)), bladder (Lopez-Knowles et al., Cancer Research 66:7401 -7404 (2006); Hennessy et al., Nature Reviews Drug Discovery 4:988- 1004 (2005)); kidney (reviewed in Downward Nature Reviews Cancer 3 : 1 1 -22 (2003)) and Head and Neck (reviewed in Engelman et al., Nature Reviews Genetics 7:606-619 (2006)).
[0006] Other classes of disorders with aberrant PI3K pathway signaling where the molecules defined within this invention may have a therapeutic benefit include inflammatory and cardiovascular diseases, including but not limited to allergies/anaphylaxis (reviewed in Rommel et al., Nature Reviews Immunology 7: 191 -201 (2007)), acute and chronic inflammation (reviewed in Ruckle et al., Nature Reviews Drug Discovery 5:903- 12 (2006); reviewed in Rommel et al., Nature Reviews Immunology 7: 191-201 (2007)), rheumatoid arthritis (reviewed in Rommel et al., Nature Reviews Immunology 7: 191 - 201 (2007)); autoimmunity disorders (reviewed in Ruckle et al., Nature Reviews Drug Discovery 5:903- 12 (2006)), thrombosis (Jackson et al., Nature Medicine 1 1 :507- 14 (2005); reviewed in Ruckle et al., Nature Reviews Drug Discovery 5:903- 12 (2006)), hypertension (reviewed in Ruckle et al., Nature Reviews Drug Discovery 5:903- 12 (2006)), cardiac hypertrophy (reviewed in Proud et al., Cardiovascular Research 63:403-13 (2004)), and heart failure (reviewed in Mocanu et al., British Journal of Pharmacology 150:833-8 (2007)).
[0007] Vacuolar Protein Sorting 34 (VPS34) is the sole Class III PI3K family member. VPS34 functions in the formation and trafficking of multiple intracellular vesicles, including vacuoles, endosomes, multivessicular bodies, lysosomes and autophagosomes (reviewed in Backer Biochem J 2008; Yan and Backer Biochem J 2007). VPS34 carries out these activities by phosphorylating Ptdlns forming PtdIns3P, resulting in the recruitment and localization of a variety of FYVE and PX domain containing effector proteins that facilitate vesicular formation, elongation and movement. At a cellular level, inhibition of VPS34 results in defects in protein sorting and autophagy. Broadly defined, autophagy is a regulated process whereby cells catabolize subcellular components targeted for degradation by enclosing them in double -membrane vesicles which then fuse with lysosomes. Autophagy has been best characterized as occurring during times of nutrient deprivation, but also plays a role in normal cellular and tissue homeostasis and functions, including the development of multiple tissue types, the immune response, clearance of neuronal aggregates and tumor suppression. In addition to functioning in vesicle formation and movement, VPS34 may also participate in several signal transduction pathways (reviewed in Backer Biochem J 2008). Given that VPS34 plays an important role in many critical cellular processes including autophagy, inhibitors of VPS34 may have therapeutic application in a number of diseases, including but not limited to cancer, muscular disorders, neurodegeneration, inflammatory disease, infectious disease and other age related illnesses (reviewed in Shintani and Klionsky Science 2004; Kondo et al Nat Rev Cancer 2005; Delgato et al Immunol Rev 2009).
[0008] Clearly, it would be beneficial to provide novel VPS34 and/or PI3K inhibitors that possess good therapeutic properties, especially for the treatment of proliferative, inflammatory, or cardiovascular disorders.
[0009] 1. General Description of Compounds of the Invention:
[0010] This invention provides compounds that are inhibitors of VPS34 and/or PI3K, and accordingly are useful for the treatment of proliferative, inflammatory, or cardiovascular disorders. The compounds of this invention are represented by formula IA:
Figure imgf000004_0001
IA
or a pharmaceutically acceptable salt thereof, wherein: -G1-G2-G3-G4- is -N=C-N-CR =, =CR -N-C=N-, =N-C=C-NR -, or -NR -C=C-N=; each occurrence of R14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
each occurrence of R3 is independently hydrogen, -CN, halogen, -Z-R5, or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
-N(R3a)-, -S-, -S(O)-, -S(0)2-, -C(O)-, -CO2-, -C(0)NR3a-, -N(R3a)C(0)-, -N(R3a)C02-, -S(0)2NR3a-, -N(R3a)S(0)2-, -OC(0)N(R3a)-, -N(R3a)C(0)NR3a-,
-N(R3a)S(0)2N(R3a)-, or -OC(O)-;
R3a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R1 is -CN, -C(0)N(R4)2, -C(0)OR4, -C(NH)N(R4)2, -NHCOR4, -NHS02R4, -NHCON(R4)2, - NHCOOR4, -NHS02N(R4)2, -CH2OH, -CH2N(R4)2, -CH2NHC(0)CH3, -S02NR4 2, -CONHC(=NH)N(R4)2, -NHS02OR4, or CY, wherein CY is an optionally substituted group selected from a 3-7-membered cycloaliphatic; a 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; a 5-6-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein:
R4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3-10- membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R4 is -Z2-R6 wherein:
Z2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-,
-S(0)2-, -C(O)-, -CO2-, -C(0)NR4a-, -C(NH)-, or -S(0)2NR4a-,
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH2, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R , taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R2 is optionally substituted with 1 -4 occurrences of R2a, wherein each occurrence of R2a is independently -R12a, -T2-R12d, -T2-R12a, or
-V2-T2-R12d, and:
each occurrence of R12a is independently halogen, -CN, -N02, -R12c, -N(R12b)2,
-OR12b, -SR12c, -S(0)2R12c, -C(0)R12b, -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2,
-OC(0)N(R12b)2, -N(R12e)C(0)R12b, -N(R12e)S02R12c, -N(R12e)C(0)OR12b,
-N(R12e)C(0)N(R12b)2, or -N(R12e)S02N(R12b)2, or two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered
heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; each occurrence of R12b is independently hydrogen or an optionally substituted group selected from \. aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12c is independently an optionally substituted group selected from Ci_ aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12e is independently hydrogen or an optionally substituted
Ci_6 aliphatic group;
each occurrence of V2 is independently -N(R12e)-, -0-, -S-, -S(O)-,
-S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R12e)-, -S(0)2N(R12e)-, -OC(0)N(R12e)-, -N(R12e)C(0)-, -N(R12e)S02-, -N(R12e)C(0)0-, -N(R12e)C(0)N(R12e)-,
-N(R12e)S02N(R12e)-, -OC(O)-, or -C(0)N(R12e)-0-; and
T2 is an optionally substituted \. alkylene chain wherein the alkylene chain optionally is interrupted by -N(R13)-, -0-, -S-, -S(0)-, -S(0)2-, -C(0)-, -C(0)0-, -C(0)N(R13)-,
-S(0)2N(R13)-, -OC(0)N(R13)-, -N(R13)C(0)-, -N(R13)S02-, -N(R13)C(0)0-, -N(R13)C(0)N(R13)-, - N(R13)S(0)2N(R13)-, -OC(O)-, or -C(0)N(R13)-0- or wherein T2 or a portion thereof optionally forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring, wherein R13 is hydrogen or an optionally substituted Ci_4aliphatic group; and
HY is an optionally substituted group selected from:
/ X6--X7 / TX6--X7
Figure imgf000007_0001
B , C , D , or E
N^Y8
I " N
v7y
or 6
F;
wherein each occurrence of ¾, X5, Χ , Χη, and Xg is independently -CR10 or N, provided no more than one occurrence of X4, X5, Χβ, X7, and Xg is N, and at least two occurrences of CR10 are CH;
each occurrence of Qi and Q2 is independently S, O or -NR9;
each occurrence of Yi, Y2, Y3, Y4, Y5, Υβ, Y7, and Yg is -CR10;
or wherein two adjacent occurrences of X4 and X5, Xe and X7, X7 and Xg, Yi and Qi, Y3 and Q2, or Y4 and Y5, taken together with the atom to which they are bound, form an optionally substituted fused group selected from 5-6-membered aryl, or 5-6-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein R10 is -R10b, -V R10c, -T R10b, or -Vi-Ti-R10b wherein:
Vi is -NR11-, -NRu-C(0)-, -NRU-C(S)-, -NRU-C(NRU)-, -NRuC(0)0-, - NRuC(0)NRu-, -NRuC(0)S-, -NRuC(S)0-, -NRUC(S)NRU-, -NRUC(S)S-, - NRuC(NRu)0-, -NR11 CfNR1 ^NR11 -, -NRuS(0)2-, -NRuS(0)2NRu-, -C(O)-, -C02-, - C(0)NRu-, -C(0)NRuO-, -SO2-, or -S02NRu-;
each occurrence of R10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
Ti is an optionally substituted \. alkylene chain wherein the alkylene chain optionally is interrupted by -N(RU)-, -0-, -S-, -S(O)-, -S(0)2-, -C(0)-, -C(0)0-, - C(0)N(Ru)-, -S(0)2N(Ru)-, -OC(0)N(Ru)-, -N(Ru)C(0)-, -N(Ru)S02-, - N(Rlla)C(0)0-, -N(R10a)C(O)N(R10a)-, -N(R10a)S(O)2N(R10a)-, -OC(O)-, or -C(0)N(Ru)-
0- or wherein Ti forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring;
each occurrence of R10b is independently hydrogen, halogen, -CN, -N02, -N(RU)2, -OR10a, -SR10a, -S(O)2R10a, -C(O)R10a, -C(O)OR10a, -C(0)N(Ru)2, -S(0)2N(Ru)2, -OC(0)N(Ru)2, -N(Ru)C(O)R10a, -N(Ru)SO2R10a, -N(Ru)C(O)OR10a, - N(Ru)C(0)N(Ru)2, or -N(Ru)S02N(Ru)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having
1 - 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
R10a and R1<¾ taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R11 is independently hydrogen, -C(0)Rl la, -C02Rl la , -C(0)N(Rl la)2,
-C(0)N(Rl la)-ORlla, -S02Rlla, -S02N(Rl la)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of Rl la is independently hydrogen or an optionally substituted group selected from Ci_6aliphatic, 3- 10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each occurrence of R9 is independently hydrogen, -C(0)R9a, -C02R9a, -C(0)N(R9b)2, -S02R9a, - S02N(R9b)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1 -5 heteratoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R% taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein a substituent on HY and R14, taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; and
provided that R1 is not an unsubstituted phenyl or a phenyl substituted only with one or two groups selected from methyl, tert-butyl, -CF3 or halogen; and
R1, R2, and Hy are not all simultaneously pyridyl;
provided that,
a) when Hy is unsubstituted 3-pyridinyl, R2 is unsubstituted phenyl, and R1 is -NHCOR4, then R4 is not an optionally substituted phenyl or pyridinyl;
b) when Hy is optionally substituted 3-pyridinyl, R2 is optionally substituted phenyl, and R1 - C(0)OR4, then R4 is not ethyl or tert-butyl;
c) Hy is not 5-methyl-3-phenyl-4-isoxazolyl;
d) when one of R1 and R2 is cyclopropyl and the other is optionally substituted phenyl, and R3 is - C(0)-R5 or -C(0)OR4, then R5 is not optionally substituted piperidinyl and R4 is not ethyl; e) neither R1 nor R2 is a phenyl ring substituted with optionally substituted lH-Pyrrolo[2,3- b]pyridinyl or 0-CH2-phenyl. f) neither R1 nor R2 is an unsubstituted ring selected from indolyl, or an optionally substituted ring selected from:
Figure imgf000010_0001
g) R1 and R2 are not both optionally substituted cyclopropyl;
h) when R1 and R2 are both optionally substituted phenyl, Hy is not optionally substituted quinolinyl or acridinyl;
i) R1 is not a phenyl ring substituted with optionally substituted phenyl, -C(0)-phenyl, -NH-CH2- phenyl, or -O-phenyl; or an unsubstituted ring selected from quinolinyl, dibenzoftiran, naphthyl, dibenzothiophene; or an optionally substituted ring selected from indolyl, 3H-Imidazo[4,5- b]pyridinyl, or:
Figure imgf000010_0002
j) R1 is not a pyrimidinyl or pyridinyl ring substituted with-N(H)C(H)(R20)-phenyl, wherein R20 is hydrogen or methyl;
k) R1 is not -C(0)NHR21, wherein R21 is thiazole, or -C(0)N(H)C(H)(Et)-R22 or -C(H)(Et)-R22, wherein R22 is pyridinyl or phenyl;
1) R2 is not a phenyl ring substituted with optionally substituted -NH-CH2-pyridinyl, -NH-CH2- phenyl, -NH-CH2-2,3-dihydro-lH-Indene, or -NH-C(0)-phenyl;
m) the compound is other than:
Figure imgf000010_0003
Figure imgf000011_0001
- 10-
Figure imgf000012_0001
- 11 -
Figure imgf000013_0001
- 12-
Figure imgf000014_0001
IA
or a pharmaceutically acceptable salt thereof, wherein:
-G1-G2-G3-G4- is -N=C-N-CR3=, =CR3-N-C=N-, =N-C=C-NR14-, or -NR14-C=C-N=;
each occurrence of R14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
each occurrence of R3 is independently hydrogen, -CN, halogen, -Z-R5, or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
-N(R3a)-, -S-, -S(O)-, -S(0)2-, -C(O)-, -CO2-, -C(0)NR3a-, -N(R3a)C(0)-, -N(R3a)C02-, -S(0)2NR3a-, -N(R3a)S(0)2-, -OC(0)N(R3a)-, -N(R3a)C(0)NR3a-,
-N(R3a)S(0)2N(R3a)-, or -OC(O)-;
R3a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R1 is -C(0)N(R4)2, -C(0)OR4, -C(NH)N(R4)2, -NHCOR4, -NHS02R4, -NHCON(R4)2, - NHCOOR4, -NHS02N(R4)2, -CH2OH, -CH2N(R4)2, -CH2NHC(0)CH3, -S02NR4 2, -CONHC(=NH)N(R4)2, -NHSO2OR4, or CY, wherein CY is an optionally substituted group selected from a 3-7-membered cycloaliphatic; a 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; a 5-6-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein:
R4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3- 10- membered cycloaliphatic, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R4 is -Z2-R6 wherein:
Z2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-, -S(0)2-, -C(O)-, -CO2-, -C(0)NR4\ -C(NH)-, or -S(0)2NR4\
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH2, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
two occurrences of R4, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R2 is optionally substituted with 1 -4 occurrences of R2a, wherein each occurrence of R2a is independently -R12a, -T2-R12d, -T2-R12a, or
-V2-T2-R12d, and:
each occurrence of R12a is independently halogen, -CN, -N02, -R12c, -N(R12b)2,
-OR12b, -SR12c, -S(0)2R12c, -C(0)R12b, -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2,
-OC(0)N(R12b)2, -N(R12e)C(0)R12b, -N(R12e)S02R12c, -N(R12e)C(0)OR12b,
-N(R12e)C(0)N(R12b)2, or -N(R12e)S02N(R12b)2, or two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; each occurrence of R12b is independently hydrogen or an optionally substituted group selected from Ci_C6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12c is independently an optionally substituted group selected from Ci_ aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12e is independently hydrogen or an optionally substituted
Ci_6 aliphatic group;
each occurrence of V2 is independently -N(R12e)-, -0-, -S-, -S(O)-,
-S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R12e)-, -S(0)2N(R12e)-, -OC(0)N(R12e)-,
-N(R12e)C(0)-, -N(R12e)S02-, -N(R12e)C(0)0-, -N(R12e)C(0)N(R12e)-,
-N(R12e)S02N(R12e)-, -OC(O)-, or -C(0)N(R12e)-0-; and
T2 is an optionally substituted \. alkylene chain wherein the alkylene chain optionally is interrupted by -N(R13)-, -0-, -S-, -S(0)-, -S(0)2-, -C(0)-, -C(0)0-, -C(0)N(R13)-,
-S(0)2N(R13)-, -OC(0)N(R13)-, -N(R13)C(0)-, -N(R13)S02-, -N(R13)C(0)0-, -N(R13)C(0)N(R13)-, - N(R13)S(0)2N(R13)-, -0C(0)-, or -C(0)N(R13)-0- or wherein T2 or a portion thereof optionally forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring, wherein R13 is hydrogen or an optionally substituted Ci_4aliphatic group; and
HY is an o tionall substituted rou selected from:
Figure imgf000016_0001
B , C , D , or E
Figure imgf000016_0002
F;
wherein each occurrence of X4, X5, Χβ, Χη, and Xg is independently -CR10 or N, provided no more than one occurrence of X4, X5, Χβ, X7, and Xg is N, and at least two occurrences of CR10 are CH; each occurrence of Qi and Q2 is independently S, O or -NR9;
each occurrence of Yi, Y2, Y3, Y4, Y5, Υβ, Y7, and Yg is -CR10;
or wherein two adjacent occurrences of ¾ and X5, Xe and X7, X7 and Xg, Yi and Qi, Y3 and Q2, or Y and Y5, taken together with the atom to which they are bound, form an optionally substituted fused group selected from 5-6-membered aryl, or 5-6-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein R10 is -R10b, -V R10c, -T R10b, or -Vi-Ti-R10b wherein:
Vi is -NR11-, -NRu-C(0)-, -NRU-C(S)-, -NRU-C(NRU)-, -NRuC(0)0-, - NRuC(0)NRu-, -NRuC(0)S-, -NRuC(S)0-, -NRUC(S)NRU-, -NRUC(S)S-, - NRuC(NRu)0-, -NR11 CfNR1 ^NR11 -, -NRuS(0)2-, -N^^^NR11-, -C(O)-, -C02-, - C(0)NRu-, -C(0)NRuO-, -SO2-, or -S02NRu-;
each occurrence of R10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
Ti is an optionally substituted C C alkylene chain wherein the alkylene chain optionally is interrupted by -N(RU)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, - C(0)N(Ru)-, -S(0)2N(Ru)-, -OC(0)N(Ru)-, -N(Ru)C(0)-, -N(Ru)S02-, - N(Rlla)C(0)0-, -N(R10a)C(O)N(R10a)-, -N(R10a)S(O)2N(R10a)-, -OC(O)-, or -C(0)N(Ru)-
0- or wherein Ti forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring;
each occurrence of R10b is independently hydrogen, halogen, -CN, -N02, -N(RU)2, -OR10a, -SR10a, -S(O)2R10a, -C(O)R10a, -C(O)OR10a, -C(0)N(Ru)2, -S(0)2N(Ru)2, -OC(0)N(Ru)2, -N(Ru)C(O)R10a, -N(Ru)SO2R10a, -N(Ru)C(O)OR10a, - N(Ru)C(0)N(Ru)2, or -N(Ru)S02N(Ru)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having
1 - 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
R10a and R1<¾ taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R11 is independently hydrogen, -C(0)Rl la, -C02RUa , -C(0)N(Rl la)2,
-C(0)N(Rl la)-ORlla, -S02RUa, -S02N(Rl la)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of Rl la is independently hydrogen or an optionally substituted group selected from Ci_6aliphatic, 3-10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R9 is independently hydrogen, -C(0)R9a, -C02R9a, -C(0)N(R9b)2, -S02R9a, - S02N(R9b)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1 -5 heteratoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R% taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein a substituent on HY and R , taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; and
provided that R1 is not an unsubstituted phenyl or a phenyl substituted only with one or two groups selected from methyl, tert-butyl, -CF3 or halogen; and
R1, R2, and Hy are not all simultaneously pyridyl;
provided that,
a) when Hy is unsubstituted 3-pyridinyl, R2 is unsubstituted phenyl, and R1 is -NHCOR4, then R4 is not an optionally substituted phenyl or pyridinyl;
b) when Hy is optionally substituted 3-pyridinyl, R2 is optionally substituted phenyl, and R1 - C(0)OR4, then R4 is not ethyl or tert-butyl;
c) Hy is not 5-methyl-3-phenyl-4-isoxazolyl;
d) when one of R1 and R2 is cyclopropyl and the other is optionally substituted phenyl, and R3 is -C(0)-R5 or -C(0)OR4, then R5 is not optionally substituted piperidinyl and R4 is not ethyl; e) neither R1 nor R2 is a phenyl ring substituted with optionally substituted lH-Pyrrolo[2,3- b]pyridinyl or 0-CH2-phenyl.
f) neither R1 nor R2 is an unsubstituted ring selected from indolyl, or an optionally substituted
Figure imgf000019_0001
R1 and R2 are not both optionally substituted cyclopropyl;
when R1 and R2 are both optionally substituted phenyl, Hy is not optionally substituted quinolinyl or acridinyl;
R1 is not a phenyl ring substituted with optionally substituted phenyl, -C(0)-phenyl, -NH- CH2-phenyl, or -O-phenyl; or an unsubstituted ring selected from quinolinyl, dibenzofuran, naphthyl, dibenzothiophene; or an optionally substituted ring selected from indolyl, 3H- Imidazo[4,5-b]pyridinyl, or:
Figure imgf000019_0002
R1 is not a pyrimidinyl or pyridinyl ring substituted with-N(H)C(H)(R20)-phenyl, wherein R is hydrogen or methyl; k) R1 is not -C(0)NHR21, wherein R21 is thiazole, or -C(0)N(H)C(H)(Et)-R22 or -C(H)(Et)-R22, wherein R22 is pyridinyl or phenyl;
1) R2 is not a phenyl ring substituted with optionally substituted -NH-CH2-pyridinyl, -NH-CH2- phenyl, -NH-CH2-2,3-dihydro-lH-Indene, or -NH-C(0)-phenyl;
m) the compound is other than:
Figure imgf000020_0001
Figure imgf000021_0001
-20-
Figure imgf000022_0001
-21 -
Figure imgf000023_0001
[0012] In another aspect, the compounds of this invention are represented by formula IA:
Figure imgf000023_0002
IA
or a pharmaceutically acceptable salt thereof, wherein:
-G1-G2-G3-G4- is -N=C-N-CR3=, =CR3-N-C=N-, =N-C=C-NR14-, or -NR14-C=C-N=;
each occurrence of R14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
each occurrence of R3 is independently hydrogen, -CN, halogen, -Z-R5, or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
-N(R3a)-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C02-, -C(0)NR3\ -N(R3a)C(0)-, -N(R3a)C02-, -S(0)2NR3a-, -N(R3a)S(0)2-, -OC(0)N(R3a)-, -N(R3a)C(0)NR3a-,
-N(R3a)S(0)2N(R3a)-, or -OC(O)-;
R3a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R1 is -CN, -C(0)N(R4)2, -C(0)OR41, -C(NH)N(R4)2, -NHCOR4, -NHS02R4, -NHCON(R4)2, - NHCOOR4, -NHS02N(R4)2, -CH2OH, -CH2N(R4)2, -CH2NHC(0)CH3, -S02NR4 2, -CONHC(=NH)N(R4)2, -NHS02OR4, or CY, wherein CY is an optionally substituted group selected from a 3-7-membered cycloaliphatic; a 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; a 5-6-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein:
R41 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3-10- membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R4 is -Z2-R6 wherein:
Z2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-,
-S(0)2-, -C(O)-, -C02-, -C(0)NR4\ -C(NH)-, or -S(0)2NR4\
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH2, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
two occurrences of R4, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4-10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R2 is optionally substituted with 1 -4 occurrences of R2a, wherein each occurrence of R2a is independently -R12a, -T2-R12d, -T2-R12a, or
-V2-T2-R12d, and: each occurrence of R12a is independently halogen, -CN, -N02, -R , -N(R12b)2, -OR12b, -SR12c, -S(0)2R12c, -C(0)R12b, -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2,
-OC(0)N(R12b)2, -N(R12e)C(0)R12b, -N(R12e)S02R12c, -N(R12e)C(0)OR12b,
-N(R12e)C(0)N(R12b)2, or -N(R12e)S02N(R12b)2, or two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered
heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; each occurrence of R12b is independently hydrogen or an optionally substituted group selected from \. aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12c is independently an optionally substituted group selected from Ci_ C6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12e is independently hydrogen or an optionally substituted
Ci_6 aliphatic group;
each occurrence of V2 is independently -N(R12e)-, -0-, -S-, -S(O)-,
-S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R12e)-, -S(0)2N(R12e)-, -OC(0)N(R12e)-,
-N(R12e)C(0)-, -N(R12e)S02-, -N(R12e)C(0)0-, -N(R12e)C(0)N(R12e)-,
-N(R12e)S02N(R12e)-, -OC(O)-, or -C(0)N(R12e)-0-; and
T2 is an optionally substituted \. alkylene chain wherein the alkylene chain optionally is interrupted by -N(R13)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R13)-,
-S(0)2N(R13)-, -OC(0)N(R13)-, -N(R13)C(0)-, -N(R13)S02-, -N(R13)C(0)0-, -N(R13)C(0)N(R13)-, - N(R13)S(0)2N(R13)-, -OC(O)-, or -C(0)N(R13)-0- or wherein T2 or a portion thereof optionally forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring, wherein R13 is hydrogen or an optionally substituted Ci_4aliphatic group; and
HY is an optionally substituted group selected from:
Figure imgf000026_0001
F;
wherein each occurrence of X4, X5, Χβ, X7, and Xg is independently -CR or N, provided no more than one occurrence of X4, X5, Χβ, X7, and Xg is N, and at least two occurrences of CR10 are CH;
each occurrence of Qi and Q2 is independently S, O or -NR9;
each occurrence of Yi, Y2, Y3, Y4, Y5, Υβ, Y7, and Yg is -CR10;
or wherein two adjacent occurrences of X4 and X5, Xe and X7, X7 and Xg, Yi and Qi, Y3 and Q2, or Y and Y5, taken together with the atom to which they are bound, form an optionally substituted fused group selected from 5-6-membered aryl, or 5-6-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
10b
wherein R is -R -V R , -T R1Ub, or -Vi-Ti-R1Ub wherein:
Vi is -NR11-, -NRu-C(0)-, -NRU-C(S)-, -NRU-C(NRU)-, -NRuC(0)0-, - NRuC(0)NRu-, -NRuC(0)S-, -NRuC(S)0-, -NRUC(S)NRU-, -NRUC(S)S-, - NRuC(NRu)0-, -NR11 CfNR1 ^NR11 -, -NRuS(0)2-, -NR^CO^NR11-, -C(O)-, -C02-, - C(0)NRu-, -C(0)NRuO-, -S02-, or -S02NRu-;
each occurrence of R10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
Ti is an optionally substituted Ci_C6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(RU)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, - C(0)N(Ru)-, -S(0)2N(Ru)-, -OC(0)N(Ru)-, -N(Ru)C(0)-, -N(Ru)S02-, - N(Rlla)C(0)0-, -N(R10a)C(O)N(R10a)-, -N(R10a)S(O)2N(R10a)-, -OC(O)-, or -C(0)N(Ru)- O- or wherein Ti forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring; each occurrence of R is independently hydrogen, halogen, -CN, -NO2,
-N(RU)2, -OR10a, -SR10a, -S(O)2R10a, -C(O)R10a, -C(O)OR10a, -C(0)N(Ru)2, -S(0)2N(Ru)2, -OC(0)N(Ru)2, -N(Ru)C(O)R10a, -N(Ru)SO2R10a, -N(Ru)C(O)OR10a, - N(Ru)C(0)N(Ru)2, or -N(Ru)S02N(Ru)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
R10a and R1<¾ taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R11 is independently hydrogen, -C(0)Rl la, -C02Rl la , -C(0)N(Rl la)2,
-C(0)N(Rl la)-ORlla, -S02Rlla, -S02N(Rl la)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of Rl la is independently hydrogen or an optionally substituted group selected from Ci_6aliphatic, 3-10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R9 is independently hydrogen, -C(0)R9a, -C02R9a, -C(0)N(R9b)2, -S02R9a, - S02N(R9b)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1 -5 heteratoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R% taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein a substituent on HY and R14, taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; and
provided that R1 is not an unsubstituted phenyl or a phenyl substituted only with one or two groups selected from methyl, tert-butyl, -CF3 or halogen; and
R1, R2, and Hy are not all simultaneously pyridyl;
provided that,
a) when Hy is unsubstituted 3-pyridinyl, R2 is unsubstituted phenyl, and R1 is -NHCOR4, then R4 is not an optionally substituted phenyl or pyridinyl;
b) when Hy is optionally substituted 3-pyridinyl, R2 is optionally substituted phenyl, and R1 - C(0)OR4, then R4 is not ethyl or tert-butyl;
c) Hy is not 5-methyl-3-phenyl-4-isoxazolyl;
d) when one of R1 and R2 is cyclopropyl and the other is optionally substituted phenyl, and R3 is -C(0)-R5 or -C(0)OR4, then R5 is not optionally substituted piperidinyl and R4 is not ethyl; e) neither R1 nor R2 is a phenyl ring substituted with optionally substituted lH-Pyrrolo[2,3- b]pyridinyl or 0-CH2-phenyl.
f) neither R1 nor R2 is an unsubstituted ring selected from indolyl, or an optionally substituted
Figure imgf000028_0001
g) R1 and R2 are not both optionally substituted cyclopropyl;
h) when R1 and R2 are both optionally substituted phenyl, Hy is not optionally substituted
quinolinyl or acridinyl; i) R1 is not a phenyl ring substituted with optionally substituted phenyl, -C(0)-phenyl, -NH- CH2-phenyl, or -O-phenyl; or an unsubstituted ring selected from quinolinyl, dibenzofuran, naphthyl, dibenzothiophene; or an optionally substituted ring selected from indolyl, 3H- Imidazo[4,5-b]pyridinyl, or:
Figure imgf000029_0001
j) R1 is not a pyrimidinyl or pyridinyl ring substituted with-N(H)C(H)(R20)-phenyl, wherein R21 is hydrogen or methyl;
k) R1 is not -C(0)NHR21, wherein R21 is thiazole, or -C(0)N(H)C(H)(Et)-R22 or -C(H)(Et)-R22, wherein R22 is pyridinyl or phenyl;
1) R2 is not a phenyl ring substituted with optionally substituted -NH-CH2-pyridinyl, -NH-CH2- phenyl, -NH-CH2-2,3-dihydro-lH-Indene, or -NH-C(0)-phenyl;
m) the compound is other than:
Figure imgf000029_0002
Figure imgf000030_0001
-29-
Figure imgf000031_0001
Figure imgf000032_0001
Figure imgf000033_0001
IA
or a pharmaceutically acceptable salt thereof, wherein:
-G1-G2-G3-G4- is -N=C-N-CR3=, =CR3-N-C=N-, =N-C=C-NR14-, or -NR14-C=C-N=;
each occurrence of R14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
each occurrence of R3 is independently hydrogen, -CN, halogen, -Z-R5, or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
-N(R3a)-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C02-, -C(0)NR3a-, -N(R3a)C(0)-, -N(R3a)C02-, -S(0)2NR3a-, -N(R3a)S(0)2-, -OC(0)N(R3a)-, -N(R3a)C(0)NR3a-,
-N(R3a)S(0)2N(R3a)-, or -OC(O)-;
R3a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R1 is -CN, -C(0)N(R4)2, -C(0)OR4, -C(NH)N(R4)2, -NHCOR4, -NHS02R4, -NHCON(R4)2, - NHCOOR4, -NHS02N(R4)2, -CH2OH, -CH2N(R4)2, -CH2NHC(0)CH3, -S02NR4 2, -CONHC(=NH)N(R4)2, -NHS02OR4, or CY, wherein CY is an optionally substituted group selected from a 3-7-membered cycloaliphatic; a 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; a 5-6-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein:
R4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3-10- membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R4 is -Z2-R6 wherein:
Z2 is selected from an optionally substituted Ci_3 alkylene chain, -S(O)-,
-S(0)2-, -C(O)-, -C02-, -C(0)NR4a-, -C(NH)-, or -S(0)2NR4a-, R a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH2, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
two occurrences of R4, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R2 is optionally substituted with 1 -4 occurrences of R2a, wherein each occurrence of R2a is independently -R12a, -T2-R12d, -T2-R12a, or
-V2-T2-R12d, and:
each occurrence of R12a is independently halogen, -CN, -N02, -R12c, -N(R12b)2,
-OR12b, -SR12c, -S(0)2R12c, -C(0)R12b, -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2,
-OC(0)N(R12b)2, -N(R12e)C(0)R12b, -N(R12e)S02R12c, -N(R12e)C(0)OR12b,
-N(R12e)C(0)N(R12b)2, or -N(R12e)S02N(R12b)2, or two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered
heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; each occurrence of R12b is independently hydrogen or an optionally substituted group selected from \. aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12c is independently an optionally substituted group selected from Ci_ aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12e is independently hydrogen or an optionally substituted
Ci_6 aliphatic group;
each occurrence of V2 is independently -N(R e)-, -0-, -S-, -S(O)-,
-S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R12e)-, -S(0)2N(R12e)-, -OC(0)N(R12e)-,
-N(R12e)C(0)-, -N(R12e)S02-, -N(R12e)C(0)0-, -N(R12e)C(0)N(R12e)-,
-N(R12e)S02N(R12e)-, -OC(O)-, or -C(0)N(R12e)-0-; and
T2 is an optionally substituted \. alkylene chain wherein the alkylene chain optionally is interrupted by -N(R13)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R13)-,
-S(0)2N(R13)-, -OC(0)N(R13)-, -N(R13)C(0)-, -N(R13)S02-, -N(R13)C(0)0-, -N(R13)C(0)N(R13)-, - N(R13)S(0)2N(R13)-, -OC(O)-, or -C(0)N(R13)-0- or wherein T2 or a portion thereof optionally forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring, wherein R13 is hydrogen or an optionally substituted Ci_4aliphatic group; and
Figure imgf000035_0001
B , C , D , or E
Figure imgf000035_0002
F;
wherein each occurrence of X4, X5, X6, X7, and X8 is independently -CR10 or N, provided no more than one occurrence of X4, X5, X6, X7, and Xg is N, and at least two occurrences of CR10 are CH;
each occurrence of Qi and Q2 is independently S, O or -NR9;
each occurrence of Yi, Y2, Y3, Y4, Y5, Υβ, Ύη, and Yg is -CR10;
or wherein two adjacent occurrences of X4 and X5, Xe and X7, X7 and Xg, Yi and Qi, Y3 and Q2, or Y4 and Y5, taken together with the atom to which they are bound, form an optionally substituted fused group selected from 5-6-membered aryl, or 5-6-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein R10 is -R10b, -V R10c, -T R10b, or -V T R1015 wherein: Vi is -NR11-, -NRu-C(0)-, -NRU-C(S)-, -NRU-C(NRU)-, -NRuC(0)0-, - NRuC(0)NRu-, -NRuC(0)S-, -NRuC(S)0-, -NRUC(S)NRU-, -NRUC(S)S-, - NRuC(NRu)0-, -NR11 CfNR1 ^NR11 -, -NRuS(0)2-, -N^^^NR11-, -C(O)-, -C02-, - C(0)NRu-, -C(0)NRuO-, -SO2-, or -S02NRu-;
each occurrence of R10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
Ti is an optionally substituted \. alkylene chain wherein the alkylene chain optionally is interrupted by -N(RU)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, - C(0)N(Ru)-, -S(0)2N(Ru)-, -OC(0)N(Ru)-, -N(Ru)C(0)-, -N(Ru)S02-, - N(Rlla)C(0)0-, -N(R10a)C(O)N(R10a)-, -N(R10a)S(O)2N(R10a)-, -OC(O)-, or -C(0)N(Ru)-
0- or wherein Τχ forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring;
each occurrence of R10b is independently hydrogen, halogen, -CN, -N02, -N(RU)2, -OR10a, -SR10a, -S(O)2R10a, -C(O)R10a, -C(O)OR10a, -C(0)N(Ru)2, -S(0)2N(Ru)2, -OC(0)N(Ru)2, -N(Ru)C(O)R10a, -N(Ru)SO2R10a, -N(Ru)C(O)OR10a, - N(Ru)C(0)N(Ru)2, or -N(Ru)S02N(Ru)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having
1 - 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
R10a and R1<¾ taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R11 is independently hydrogen, -C(0)Rl la, -C02Rl la , -C(0)N(Rl la)2,
-C(0)N(Rl la)-ORlla, -S02Rlla, -S02N(Rl la)2, or an optionally substituted group selected from Cw aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of Rl la is independently hydrogen or an optionally substituted group selected from Ci_6aliphatic, 3-10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R9 is independently hydrogen, -C(0)R9a, -C02R9a, -C(0)N(R9b)2, -S02R9a, - S02N(R9b)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1 -5 heteratoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R% taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein a substituent on HY and R14, taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; and
provided that R1 is not an optionally substituted phenyl; and
R1, R2, and Hy are not all simultaneously pyridyl;
provided that,
a) when Hy is unsubstituted 3-pyridinyl, R2 is unsubstituted phenyl, and R1 is -NHCOR4, then R4 is not an optionally substituted phenyl or pyridinyl; b) when Hy is optionally substituted 3-pyridinyl, R2 is optionally substituted phenyl, and R1 - C(0)OR4, then R4 is not ethyl or tert-butyl;
c) Hy is not 5-methyl-3-phenyl-4-isoxazolyl;
d) when one of R1 is cyclopropyl and R2 is optionally substituted phenyl, and R3 is -C(0)-R5 or -C(0)OR4, then R5 is not optionally substituted piperidinyl and R4 is not ethyl;
e) R2 is not a phenyl ring substituted with optionally substituted lH-Pyrrolo[2,3-b]pyridinyl or 0-CH2-phenyl;
f) neither R1 nor R2 is an unsubstituted ring selected from indolyl, or an optionally substituted ring selected from:
Figure imgf000038_0001
g) R1 and R2 are not both optionally substituted cyclopropyl;
h) R1 is not a pyrimidinyl or pyridinyl ring substituted with-N(H)C(H)(R20)-phenyl, wherein R21 is hydrogen or methyl;
i) R1 is not -C(0)NHR21, wherein R21 is thiazole, or -C(0)N(H)C(H)(Et)-R22 or -C(H)(Et)-R22, wherein R22 is pyridinyl or phenyl;
j) R2 is not a phenyl ring substituted with optionally substituted -NH-CH2-pyridinyl, -NH-CH2- phenyl, -NH-CH2-2,3-dihydro-lH-Indene, or -NH-C(0)-phenyl;
k) the compound is other than:
Figure imgf000038_0002
Figure imgf000039_0001
-38-
Figure imgf000040_0001
Figure imgf000041_0001
[0014] In another aspect, the compounds of this invention are represented by formula IA:
Figure imgf000041_0002
IA
or a pharmaceutically acceptable salt thereof, wherein:
-G1-G2-G3-G4- is -N=C-N-CR3=, =CR3-N-C=N-, =N-C=C-NR14-, or -NR14-C=C-N=;
each occurrence of R14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
each occurrence of R3 is independently hydrogen, -CN, halogen, -Z-R5, or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
-N(R3a)-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C02-, -C(0)NR3\ -N(R3a)C(0)-, -N(R3a)C02-, -S(0)2NR3a-, -N(R3a)S(0)2-, -OC(0)N(R3a)-, -N(R3a)C(0)NR3a-,
-N(R3a)S(0)2N(R3a)-, or -OC(O)-;
R3a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R1 is -CN, -C(0)N(R4)2, -C(0)OR4, -C(NH)N(R4)2, -NHCOR4, -NHCOOR4, -NHS02N(R4)2, - CH2OH, -CH2N(R4)2, -CH2NHC(0)CH3, -S02NR4 2, -CONHC(=NH)N(R4)2, -NHS02OR4, or CY, wherein CY is an optionally substituted group selected from a 3-7-membered cycloaliphatic; a 4-10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; a 5-6-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein:
R4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3-10- membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R4 is -Z2-R6 wherein:
Z2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-,
-S(0)2-, -C(O)-, -CO2-, -C(0)NR4\ -C(NH)-, or -S(0)2NR4\
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH2, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
two occurrences of R4, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4-10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R2 is optionally substituted with 1 -4 occurrences of R2a, wherein each occurrence of R2a is independently -R12a, -T2-R12d, -T2-R12a, or
-V2-T2-R12d, and:
each occurrence of R12a is independently halogen, -CN, -N02, -R12c, -N(R12b)2,
-OR12b, -SR12c, -S(0)2R12c, -C(0)R12b, -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2,
-OC(0)N(R12b)2, -N(R12e)C(0)R12b, -N(R12e)S02R12c, -N(R12e)C(0)OR12b,
-N(R12e)C(0)N(R12b)2, or -N(R12e)S02N(R12b)2, or two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered
heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; each occurrence of R12b is independently hydrogen or an optionally substituted group selected from Ci_C6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each occurrence of R c is independently an optionally substituted group selected from Ci_ aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12e is independently hydrogen or an optionally substituted
Ci_6 aliphatic group;
each occurrence of V2 is independently -N(R12e)-, -0-, -S-, -S(O)-,
-S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R12e)-, -S(0)2N(R12e)-, -OC(0)N(R12e)-,
-N(R12e)C(0)-, -N(R12e)S02-, -N(R12e)C(0)0-, -N(R12e)C(0)N(R12e)-,
-N(R12e)S02N(R12e)-, -OC(O)-, or -C(0)N(R12e)-0-; and
T2 is an optionally substituted Ci_C6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R13)-, -0-, -S-, -S(0)-, -S(0)2-, -C(0)-, -C(0)0-, -C(0)N(R13)-,
-S(0)2N(R13)-, -OC(0)N(R13)-, -N(R13)C(0)-, -N(R13)S02-, -N(R13)C(0)0-, -N(R13)C(0)N(R13)-, - N(R13)S(0)2N(R13)-, -0C(0)-, or -C(0)N(R13)-0- or wherein T2 or a portion thereof optionally forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring, wherein R13 is hydrogen or an optionally substituted Ci_4aliphatic group; and
HY is an o tionall substituted rou selected from:
Figure imgf000043_0001
B , C , D , or E
Figure imgf000043_0002
F;
wherein each occurrence of X4, X5, Χβ, Χη, and Xg is independently -CR10 or N, provided no more than one occurrence of X4, X5, Χβ, X7, and Xg is N, and at least two occurrences of CR10 are CH;
each occurrence of Qi and Q2 is independently S, O or -NR9;
each occurrence of Yi, Y2, Y3, Y4, Y5, Υβ, Y7, and Yg is -CR10; or wherein two adjacent occurrences of X4 and X5, Xe and X7, X7 and Xg, Yi and Qi, Y3 and Q2, or Y4 and Y5, taken together with the atom to which they are bound, form an optionally substituted fused group selected from 5-6-membered aryl, or 5-6-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein R10 is -R10b, -V R10c, -T R10b, or -V T R1015 wherein:
Vi is -NR11-, -NRu-C(0)-, -NRU-C(S)-, -NRU-C(NRU)-, -NRuC(0)0-, - NRuC(0)NRu-, -NRuC(0)S-, -NRuC(S)0-, -NRUC(S)NRU-, -NRUC(S)S-, - NRuC(NRu)0-, -NR11 CfNR1 ^NR11 -, -NRuS(0)2-, -N^^^NR11-, -C(O)-, -C02-, - C(0)NRu-, -C(0)NRuO-, -SO2-, or -S02NRu-;
each occurrence of R10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
Ti is an optionally substituted Ci_C6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(RU)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, - C(0)N(Ru)-, -S(0)2N(Ru)-, -OC(0)N(Ru)-, -N(Ru)C(0)-, -N(Ru)S02-, - N(Rlla)C(0)0-, -N(R10a)C(O)N(R10a)-, -N(R10a)S(O)2N(R10a)-, -OC(O)-, or -C(0)N(Ru)-
0- or wherein Ti forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring;
each occurrence of R10b is independently hydrogen, halogen, -CN, -N02, -N(RU)2, -OR10a, -SR10a, -S(O)2R10a, -C(O)R10a, -C(O)OR10a, -C(0)N(Ru)2, -S(0)2N(Ru)2, -OC(0)N(Ru)2, -N(Ru)C(O)R10a, -N(Ru)SO2R10a, -N(Ru)C(O)OR10a, - N(Ru)C(0)N(Ru)2, or -N(Ru)S02N(Ru)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having
1 - 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or R a and R ; taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R11 is independently hydrogen, -C(0)Rl la, -C02RUa , -C(0)N(Rl la)2,
-C(0)N(Rl la)-ORlla, -S02RUa, -S02N(Rl la)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of Rl la is independently hydrogen or an optionally substituted group selected from Ci_6aliphatic, 3-10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R9 is independently hydrogen, -C(0)R9a, -C02R9a, -C(0)N(R9b)2, -S02R9a, - S02N(R9b)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteratoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1 -5 heteratoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R% taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein a substituent on HY and R14, taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; and provided that R is not an unsubstituted phenyl or a phenyl substituted only with one or two groups selected from methyl, tert-butyl, -CF3 or halogen; and
R1, R2, and Hy are not all simultaneously pyridyl;
provided that,
a) when Hy is unsubstituted 3-pyridinyl, R2 is unsubstituted phenyl, and R1 is -NHCOR4, then R4 is not an optionally substituted phenyl or pyridinyl;
b) when Hy is optionally substituted 3-pyridinyl, R2 is optionally substituted phenyl, and R1 - C(0)OR4, then R4 is not ethyl or tert-butyl;
c) Hy is not 5-methyl-3-phenyl-4-isoxazolyl;
d) when one of R1 and R2 is cyclopropyl and the other is optionally substituted phenyl, and R3 is -C(0)-R5 or -C(0)OR4, then R5 is not optionally substituted piperidinyl and R4 is not ethyl; e) neither R1 nor R2 is a phenyl ring substituted with optionally substituted lH-Pyrrolo[2,3- b]pyridinyl or 0-CH2-phenyl.
f) neither R1 nor R2 is an unsubstituted ring selected from indolyl, or an optionally substituted
Figure imgf000046_0001
R1 and R2 are not both optionally substituted cyclopropyl;
when R1 and R2 are both optionally substituted phenyl, Hy is not optionally substituted quinolinyl or acridinyl;
R1 is not a phenyl ring substituted with optionally substituted phenyl, -C(0)-phenyl, -NH- CH2-phenyl, or -O-phenyl; or an unsubstituted ring selected from quinolinyl, dibenzofuran, naphthyl, dibenzothiophene; or an optionally substituted ring selected from indolyl, 3H- Imidazo[4,5-b]pyridinyl, or:
Figure imgf000046_0002
R1 is not a pyrimidinyl or pyridinyl ring substituted with-N(H)C(H)(R20)-phenyl, wherein R21 is hydrogen or methyl;
R1 is not -C(0)NHR21, wherein R21 is thiazole, or -C(0)N(H)C(H)(Et)-R22 or -C(H)(Et)-R22, wherein R22 is pyridinyl or phenyl;
R2 is not a phenyl ring substituted with optionally substituted -NH-CH2-pyridinyl, -NH-CH2- phenyl, -NH-CH2-2,3-dihydro-lH-Indene, or -NH-C(0)-ph< the compound is other than:
Figure imgf000047_0001
Figure imgf000048_0001
-47-
Figure imgf000049_0001
-48-
Figure imgf000050_0001
[0015] In certain other embodiments, compounds of formula VA are provided:
Figure imgf000050_0002
VA or a pharmaceutically acceptable salt thereof, wherein:
R14 is independently hydrogen, or an optionally substituted group selected from Ci_6 aliphatic and Ci_3 cycloalkyl;
each occurrence of R3 is independently hydrogen, -CN, halogen, -Z-R5, or an optionally substituted group selected from Ci_6 aliphatic and 3-10-membered cycloaliphatic, wherein:
Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
-N(R3a)-, -S-, -S(O)-, -S(0)2-, -C(O)-, -CO2-, -C(0)NR3\ -N(R3a)C(0)-, -N(R3a)C02-, -S(0)2NR3a-, -N(R3a)S(0)2-, -OC(0)N(R3a)-, -N(R3a)C(0)NR3a-,
-N(R3a)S(0)2N(R3a)-, or -OC(O)-;
R3a is hydrogen or an optionally substituted Ci_4 aliphatic, and R5 is an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R1 is -CN, -C(0)N(R4)2, -C(0)OR4, -C(NH)N(R4)2, -NHCOR4, -NHS02R4, -NHCON(R4)2, - NHCOOR4, -NHS02N(R4)2, -CH2OH, -CH2N(R4)2, -CH2NHC(0)CH3, -S02 N(R4)2, - C(0)NHC(=NH)N(R4)2, -NHS02OR4, or CY, wherein CY is an optionally substituted group selected from a 3-7-membered cycloaliphatic; a 4- 10-membered heterocyclyl having 1-5 heteroatoms
independently selected from nitrogen, oxygen, or sulfur; a 5-6-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein:
R4 is hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3-10- membered cycloaliphatic, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or R4 is -Z2-R6 wherein:
Z2 is selected from an optionally substituted Ci_3 alkylene chain, -S(O)-, -S(0)2-, -C(O)-, -C02-, -C(0)NR4\ -C(NH)-, or -S(0)2NR4\
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen, or an optionally substituted group selected from Ci_6 aliphatic, - NH2, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
two occurrences of R4, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R2 is an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4-10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R2 is optionally substituted with 1 -4 occurrences of R2a, wherein each occurrence of R2a is independently -R12a, -T2-R12d, -T2-R12a, or
-V2-T2-R12d, and:
each occurrence of R12a is independently halogen, -CN, -N02, -R12c, -N(R12b)2,
-OR12b, -SR12c, -S(0)2R12c, -C(0)R12b, -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2, -OC(0)N(R12b)2, -N(R12e)C(0)R12b, -N(R12e)S02R , -N(R12e)C(0)OR12b,
-N(R12e)C(0)N(R12b)2, or -N(R12e)S02N(R12b)2, or two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered
heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur; each occurrence of R12b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12c is independently an optionally substituted group selected from Ci_ 6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12d is independently hydrogen or an optionally substituted group selected from 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12e is independently hydrogen or an optionally substituted
Ci_6 aliphatic group;
each occurrence of V2 is independently -N(R12e)-, -0-, -S-, -S(O)-,
-S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R12e)-, -S(0)2N(R12e)-, -OC(0)N(R12e)-,
-N(R12e)C(0)-, -N(R12e)S02-, -N(R12e)C(0)0-, -N(R12e)C(0)N(R12e)-,
-N(R12e)S02N(R12e)-, -OC(O)-, or -C(0)N(R12e)-0-; and
T2 is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R13)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R13)-,
-S(0)2N(R13)-, -OC(0)N(R13)-, -N(R13)C(0)-, -N(R13)S02-, -N(R13)C(0)0-, -N(R13)C(0)N(R13)-, -N(R13)S(0)2N(R13)-, -OC(O)-, or -C(0)N(R13)-0- or wherein T2 or a portion thereof optionally forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring, wherein R13 is hydrogen or an optionally substituted Ci_4 aliphatic group; and
HY is an optionally substituted group selected from:
Figure imgf000053_0001
F;
wherein each occurrence of X4, X5, Χβ, X7, and Xg is independently -CR or N, provided no more than one occurrence of X4, X5, Χβ, X7, and Xg is N, and at least two occurrences of CR10 are CH;
each occurrence of Qi and Q2 is independently S, O or -NR9;
each occurrence of Yi, Y2, Y3, Y4, Y5, Υβ, Y7, and Yg is -CR10;
or wherein two adjacent occurrences of X4 and X5, Xe and X7, X7 and Xg, Yi and Qi, Y3 and Q2, or Y and Y5, taken together with the atom to which they are bound, form an optionally substituted fused group selected from 5-6-membered aryl, or 5-6-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
10b
wherein R is -R -V R , -T R1Ub, or -Vi-Ti-R1Ub wherein:
Vi is -NR11-, -NR -C(O)-, -NR -C(S)-, -NR -C(N U)-, -NRuC(0)0-, - NRuC(0)NRu-, -NRuC(0)S-, -NRuC(S)0-, -NRUC(S)NRU-, -NRUC(S)S-, - NRuC(NRu)0-, -NR11 CfNR1 ^NR11 -, -NRuS(0)2-, -NR^CO^NR11-, -C(O)-, -C02-, - C(0)NRu-, -C(0)NRuO-, -SO2-, or -S02NRu-;
Ti is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(RU)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, - C(0)N(Ru)-, -S(0)2N(Ru)-, -OC(0)N(Ru)-, -N(Ru)C(0)-, -N(Ru)S02-, - N(Rlla)C(0)0-, -N(R10a)C(O)N(R10a)-, -N(R10a)S(O)2N(R10a)-, -OC(O)-, or -C(0)N(Ru)- O- or wherein Ti forms part of an optionally substituted 3-7 membered cycloaliphatic or heterocyclyl ring;
each occurrence of R10a is independently hydrogen or an optionally substituted group selected from d_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each occurrence of R is independently hydrogen, halogen, -CN, -NO2,
-N(RU)2, -OR10a, -SR10a, -S(O)2R10a, -C(O)R10a, -C(O)OR10a, -C(0)N(Ru)2, -S(0)2N(Ru)2, -OC(0)N(Ru)2, -N(Ru)C(O)R10a, -N(Ru)SO2R10a, -N(Ru)C(O)OR10a, - N(Ru)C(0)N(Ru)2, or -N(Ru)S02N(Ru)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
R10a and R10b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R11 is independently hydrogen, -C(0)Rl la, -C02Rl la , -C(0)N(Rl la)2,
-C(0)N(Rl la)-ORlla, -S02Rlla, -S02N(Rl la)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of Rl la is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4-10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R9 is independently hydrogen, -C(0)R9a, -C02R9a, -C(0)N(R9b)2, -S02R9a, - S02N(R9b)2, or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered
cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R% taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3-6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein a substituent on HY and R14, taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur.
[0016] In certain other embodiments, compounds of formula IA are provided:
Figure imgf000055_0001
IA or a pharmaceutically acceptable salt thereof, wherein:
-G1-G2-G3-G4- is -N=C-N-CR3=, =CR3-N-C=N-, =N-C=C-NR14-, or -NR14-C=C-N=;
each occurrence of R14 is independently hydrogen, cyclopropyl, or an optionally substituted group selected from Ci_6 aliphatic;
each occurrence of R3 is independently hydrogen, -CN, halogen, -Z-R5, or an optionally substituted group selected from Ci_6 aliphatic and 3- to 10-membered cycloaliphatic, wherein:
Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
-N(R3a)-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C02-, -C(0)NR3\ -N(R3a)C(0)-,
-N(R3a)C02-, -S(0)2NR3a-, -N(R3a)S(0)2-, -OC(0)N(R3a)-, -N(R3a)C(0)NR3a-,
-N(R3a)S(0)2N(R3a)-, or -OC(O)-;
R3a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R5 is hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10- membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R1 is -CN, -C(0)N(R4)2, -C(0)OR4, -C(NR4)N(R4)2, -NHCOR4, -NHS02R4, -NHCON(R4)2, - NHCOOR4, -NHS02N(R4)2, -CH2OR4, -CH2N(R4)2, -CH2NHC(0)R4, -S02 N(R4)2, - C(0)NHC(=NH)N(R4)2, -NHS02OR4, or CY, wherein CY is an optionally substituted group selected from a 3- to -7-membered cycloaliphatic; a 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; a 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein:
each R4 is independently selected from hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
R4 is -Z2-R6 wherein:
Z2 is selected from an optionally substituted Ci_3 alkylene chain, -S(O)-, -S(0)2-, -C(O)-, -C02-, -C(0)NR4\ -C(NH)-, or -S(0)2NR4\
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen, -NH2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
two occurrences of R4, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R2 is hydrogen, halo, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10- membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R2 is optionally substituted with 1-4 occurrences of R2a, wherein each occurrence of R2a is independently -R12a, -T2-R12d, -T2-R12a, or -V2-T2-R12d, and:
each occurrence of R12a is independently halogen, -CN, -N02, -R12c, -N(R12b)2,
-OR12b, -SR12c, -S(0)2R12c, -C(0)R12b, -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2,
-OC(0)N(R12b)2, -N(R12e)C(0)R12b, -N(R12e)S02R12c, -N(R12e)C(0)OR12b, -N(R12e)C(0)N(R12b)2, or -N(R12e)S02N(R12b)2, or an optionally substituted Ci_6 aliphatic or Ci_6 haloaliphatic;
each occurrence of R12b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
each occurrence of R12c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, Ci_6 haloaliphatic, 3- to 10-membered cycloaliphatic, 4- to 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12d is independently hydrogen or an optionally substituted group selected from 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12e is independently hydrogen or an optionally substituted
Ci_6 aliphatic group;
each occurrence of V2 is independently -N(R12e)-, -0-, -S-, -S(O)-,
-S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R12e)-, -S(0)2N(R12e)-, -OC(0)N(R12e)-,
-N(R12e)C(0)-, -N(R12e)S02-, -N(R12e)C(0)0-, -N(R12e)C(0)N(R12e)-,
-N(R12e)S02N(R12e)-, -OC(O)-, or -C(0)N(R12e)-0-; and
T2 is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R13)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R13)-,
-S(0)2N(R13)-, -OC(0)N(R13)-, -N(R13)C(0)-, -N(R13)S02-, -N(R13)C(0)0-, -N(R13)C(0)N(R13)-, - N(R13)S(0)2N(R13)-, -OC(O)-, or -C(0)N(R13)-0- or wherein T2 or a portion thereof optionally forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocyclyl ring, wherein R13 is hydrogen or an optionally substituted Ci_4 aliphatic group; and
HY is a group selected from:
Figure imgf000058_0001
A , B , C ,
Figure imgf000058_0002
E , or F;
wherein
each occurrence of X4, X5, X , X7, and Xg is independently -CR10, -CR10 , or N, provided no more than two occurrences of X4, X5, Χβ, X7, and Xg is N;
each occurrence of Yi, Y2, Y3, Y4, Y5, Υβ, Y7, and Yg is -CR10;
each occurrence of Qi and Q2 is independently S, O or -NR9;
two adjacent occurrences of X4 and X5, Xe and X7, X7 and Xg, Yi and -NR9, Y3 and - NR9, or Y4 and Y5, may be taken together with the atoms to which they are bound, to form an unsubstituted fused heteroaryl or heterocyclyl group having 8 to 10 ring atoms and having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10 or R10' is independently -R10b, -V R10c, -T R10b, or -Vi-T R10b, wherein:
Vi is -NR11-, -NRu-C(0)-, -NRU-C(S)-, -NRU-C(NRU)-, -NRuC(0)0-, - NRuC(0)NRu-, -NRuC(0)S-, -NRuC(S)0-, -NRUC(S)NRU-, -NRUC(S)S-, - NRuC(NRu)0-, -NR11 CfNR1 ^NR11 -, -NRuS(0)2-, -NR^CO^NR11-, -C(O)-, -C02-, - C(0)NRu-, -C(0)NRuO-, -SO2-, or -S02NRu-;
each occurrence of R10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
Ti is an optionally substituted C e alkylene chain wherein the alkylene chain optionally is interrupted by -N(RU)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, - C(0)N(Ru)-, -S(0)2N(Ru)-, -OC(0)N(Ru)-, -N(Ru)C(0)-, -N(Ru)S02-, - N(Rlla)C(0)0-, -N(R10a)C(O)N(R10a)-, -N(R10a)S(O)2N(R10a)-, -OC(O)-, or -C(0)N(Ru)- O- or wherein Ti forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocyclyl ring; each occurrence of R is independently hydrogen, halogen, -CN, -NO2, -N(RU)2, -OR10a, -SR10a, -S(O)2R10a, -C(O)R10a, -C(O)OR10a, -C(0)N(Ru)2,
-S(0)2N(Ru)2, -OC(0)N(Ru)2, -N(Ru)C(O)R10a, -N(Ru)SO2R10a, -N(Ru)C(O)OR10a, - N(Ru)C(0)N(Ru)2, or -N(Ru)S02N(Ru)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
R10a and R1<¾ taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R11 is independently hydrogen, -C(0)Rl la, -C02Rl la , -C(0)N(Rl la)2,
-C(0)N(Rlla)-ORlla, -S02Rl la, -S02N(Rlla)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of Rl la is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R9 is independently hydrogen, -C(0)R9a, -C02R9a, -C(0)N(R9b)2, -S02R9a, - S02N(R9b)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R% taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3- to 6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and provided that when HY is a non- fused group then HY is substituted with at least one occurrence of R10 or R10', wherein R10 or R10' is:
-N(Ru)C(O)R10a, -C(0)N(Ru)2, or -NRuC(O)OR10a; or
-Vi-T R10b, wherein Vl is -NR11-, Tt is a d -C3 alkylene chain, and R10b is an optionally substituted 6- to 10-membered aryl ring or a 5- to 10-membered heteroaryl ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or Vi is -NRuC(0)NRu- , Ti is a Ci -C3 alkylene chain, and R10b is -OR10a; or
-Vi-R10c, wherein Vi is -NR11-, and R10c is a 5- to 10-membered heteroaryl ring having 1- 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and
wherein a substituent on HY and R14, taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
provided that:
when R3 is hydrogen, then R1 is not
Figure imgf000060_0001
, wherein R103 is hydrogen or -C(0)2tBu; when R3 is hydrogen, then R1 is not -CHzOCHzCHzSiMes;
when R2 is hydrogen and Gi and G3 are nitrogen, then formula IA does not exist as the tautomeric form where Gi is substituted with hydrogen;
hydrogen, and R1 is 2-chloro-6-fluorophenyl, then HY is not
Figure imgf000061_0001
where R is an optionally substituted phenyl ring
when HY is a substituted thiazolyl ring, then R1 is not a substituted pyrrolidinyl rins
f) when R2 and R3 are both hydrogen, then HY is not
Figure imgf000061_0002
, wherein R104 is -OH or an optionally substituted phenyl ring;
g) HY is not
h) when HY
Figure imgf000061_0003
neither R1 nor R2 is a cyclopropyl ring; and
i) provided that the compound is other than:
Figure imgf000061_0004
Figure imgf000062_0001
-61 -
Figure imgf000063_0001
Figure imgf000063_0002
[0017] In certain other embodiments, compounds of formula IA are provided:
Figure imgf000063_0003
IA or a pharmaceutically acceptable salt thereof, wherein: -G1-G2-G3-G4- is -N=C-N-CR =, =CR -N-C=N-, =N-C=C-NR -, or -NR -C=C-N=; each occurrence of R14 is independently hydrogen, cyclopropyl, or an optionally substituted group selected from Ci_6 aliphatic;
each occurrence of R3 is independently hydrogen, -CN, halogen, -Z-R5, or an optionally substituted group selected from Ci_6 aliphatic and 3- to 10-membered cycloaliphatic, wherein:
Z is selected from an optionally substituted Q_3 alkylene chain, -0-,
-N(R3a)-, -S-, -S(O)-, -S(0)2-, -C(O)-, -CO2-, -C(0)NR3a-, -N(R3a)C(0)-,
-N(R3a)C02-, -S(0)2NR3a-, -N(R3a)S(0)2-, -OC(0)N(R3a)-, -N(R3a)C(0)NR3a-,
-N(R3a)S(0)2N(R3a)-, or -OC(O)-;
R3a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R5 is hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10- membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R1 is -CN, -C(0)N(R4)2, -C(0)OR4, -C(NR4)N(R4)2, -NHCOR4, -NHS02R4, -NHCON(R4)2, - NHCOOR4, -NHS02N(R4)2, -CH2OR4, -CH2N(R4)2, -CH2NHC(0)R4, -S02 N(R4)2, - C(0)NHC(=NH)N(R4)2, -NHS02OR4, or CY, wherein CY is an optionally substituted group selected from a 3- to -7-membered cycloaliphatic; a 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; a 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein:
each R4 is independently selected from hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
R4 is -Z2-R6 wherein:
Z2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-,
-S(0)2-, -C(O)-, -CO2-, -C(0)NR4a-, -C(NH)-, or -S(0)2NR4a-,
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen, -NH2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R , taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R2 is halo, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R2 is optionally substituted with 1 -4 occurrences of R2a, wherein each occurrence of R2a is independently -R12a, -T2-R12d, -T2-R12a, or -V2-T2-R12d, and:
each occurrence of R12a is independently halogen, -CN, -N02, -R12c, -N(R12b)2,
-OR12b, -SR12c, -S(0)2R12c, -C(0)R12b, -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2,
-OC(0)N(R12b)2, -N(R12e)C(0)R12b, -N(R12e)S02R12c, -N(R12e)C(0)OR12b,
-N(R12e)C(0)N(R12b)2, or -N(R12e)S02N(R12b)2, or an optionally substituted d_6 aliphatic or d_6 haloaliphatic;
each occurrence of R12b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
each occurrence of R12c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, Ci_6 haloaliphatic, 3- to 10-membered cycloaliphatic, 4- to 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12d is independently hydrogen or an optionally substituted group selected from 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12e is independently hydrogen or an optionally substituted
Ci_6 aliphatic group; each occurrence of V2 is independently -N(R12e)-, -0-, -S-, -S(0)-,
-S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R12e)-, -S(0)2N(R12e)-, -OC(0)N(R12e)-,
-N(R12e)C(0)-, -N(R12e)S02-, -N(R12e)C(0)0-, -N(R12e)C(0)N(R12e)-,
-N(R12e)S02N(R12e)-, -OC(O)-, or -C(0)N(R12e)-0-; and
T2 is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R13)-, -0-, -S-, -S(0)-, -S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R13)-,
-S(0)2N(R13)-, -OC(0)N(R13)-, -N(R13)C(0)-, -N(R13)S02-, -N(R13)C(0)0-, -N(R13)C(0)N(R13)-, - N(R13)S(0)2N(R13)-, -OC(O)-, or -C(0)N(R13)-0- or wherein T2 or a portion thereof optionally forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocyclyl ring, wherein R13 is hydrogen or an optionally substituted Ci_4 aliphatic group; and
HY is a group selected from:
Figure imgf000066_0001
E , or F;
wherein
each occurrence of ¾, X5, X6, X7, and X8 is independently -CR10, -CR10 , or N, provided no more than two occurrences of X4, X5, X6, X7, and X8 is N;
each occurrence of Yi, Y2, Y3, Y4, Y5, Y6, Y7, and Y8 is -CR10;
each occurrence of Qi and Q2 is independently S, O or -NR9;
two adjacent occurrences of X4 and X5, Xe and X7, X7 and Xg, Yi and -NR9, Y3 and - NR9, or Y4 and Y5, may be taken together with the atoms to which they are bound, to form an unsubstituted fused heteroaryl or heterocyclyl group having 8 to 10 ring atoms and having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10 or R10' is independently -R10b, -V R10c, -T R10b, or -Vi-T R10b, wherein:
Vi is -NR11-, -NRu-C(0)-, -NRU-C(S)-, -NRU-C(NRU)-, -NRuC(0)0-, - NRuC(0)NRu-, -NRuC(0)S-, -NRuC(S)0-, -NRUC(S)NRU-, -NRUC(S)S-, - NRuC(NRu)0-, -NR11 CfNR1 ^NR11 -, -NRuS(0)2-, -NRuS(0)2NRu-, -C(O)-, -C02-, - C(0)NRu-, -C(0)NRuO-, -S02-, or -S02NRu-; each occurrence of R a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
Ti is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(RU)-, -0-, -S-, -S(O)-, -S(0)2-, -C(0)-, -C(0)0-, - C(0)N(Ru)-, -S(0)2N(Ru)-, -OC(0)N(Ru)-, -N(Ru)C(0)-, -N(Ru)S02-, - N(Rlla)C(0)0-, -N(R10a)C(O)N(R10a)-, -N(R10a)S(O)2N(R10a)-, -OC(O)-, or -C(0)N(Ru)- O- or wherein Ti forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocyclyl ring;
each occurrence of R10b is independently hydrogen, halogen, -CN, -N02, -N(RU)2, -OR10a, -SR10a, -S(O)2R10a, -C(O)R10a, -C(O)OR10a, -C(0)N(Ru)2,
-S(0)2N(Ru)2, -OC(0)N(Ru)2, -N(Ru)C(O)R10a, -N(Ru)SO2R10a, -N(Ru)C(O)OR10a, - N(Ru)C(0)N(Ru)2, or -N(Ru)S02N(Ru)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
R10a and R1<¾ taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R11 is independently hydrogen, -C(0)Rl la, -C02Rl la , -C(0)N(Rl la)2,
-C(0)N(Rlla)-ORlla, -S02Rl la, -S02N(Rlla)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of Rl la is independently hydrogen or an optionally substituted group selected from C e aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R9 is independently hydrogen, -C(0)R9a, -C02R9a, -C(0)N(R9b)2, -S02R9a, - S02N(R9b)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R% taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3- to 6-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and provided that when HY is a non- fused group then HY is substituted with at least one occurrence of R10 or R10', wherein R10 or R10' is:
-N(Ru)C(O)R10a, -C(0)N(Ru)2, or -NRuC(O)OR10a; or
-Vi-T R10b, wherein Vi is -NR11-, Ti is a Ci -C3 alkylene chain, and R is an optionally substituted 6- to 10-membered aryl ring or a 5- to 10-membered heteroaryl ring having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or Vi is -NRuC(0)NRu- , Ti is a Ci -C3 alkylene chain, and R10b is -OR10a; or
-Vi-R10c, wherein Vi is -NR11-, and R10c is a 5- to 10-membered heteroaryl ring having 1 - 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and
wherein a substituent on HY and R14, taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
provided that:
a) when R3 is hydrogen, then R1 is not
Figure imgf000069_0001
, wherein R103 is hydrogen or -C(0)2tBu; b) when R3 is hydrogen, then R1 is not -CH2OCH2CH2SiMe3;
c) when HY is a substituted thiazolyl ring, then R1 is not a substituted pyrrolidinyl ring;
HY is not substituted with
Figure imgf000069_0002
Figure imgf000069_0003
when HY is neither R1 nor R2 is a cyclopropyl ring; and
provided that the compound is other than:
Figure imgf000069_0004
Figure imgf000070_0001
Figure imgf000070_0002
[0018] In certain other embodiments, compounds of formula IA are provided:
Figure imgf000070_0003
IA
or a pharmaceutically acceptable salt thereof, wherein:
-G1-G2-G3-G4- is -N=C-N-CR3=, =CR3-N-C=N-, =N-C=C-NR14-, or -NR14-C=C-N=; each occurrence of R is independently hydrogen, cyclopropyl, or an optionally substituted group selected from Ci_6 aliphatic;
each occurrence of R3 is independently hydrogen, -CN, halogen, -Z-R5, or an optionally substituted group selected from Ci_6 aliphatic and 3- to 10-membered cycloaliphatic, wherein:
Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
-N(R3a)-, -S-, -S(O)-, -S(0)2-, -C(O)-, -CO2-, -C(0)NR3a-, -N(R3a)C(0)-,
-N(R3a)C02-, -S(0)2NR3a-, -N(R3a)S(0)2-, -OC(0)N(R3a)-, -N(R3a)C(0)NR3a-,
-N(R3a)S(0)2N(R3a)-, or -OC(O)-;
R3a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R5 is hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10- membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R1 is -CN, -C(0)N(R4)2, -C(0)OR4, -C(NR4)N(R4)2, -NHCOR4, -NHS02R4, -NHCON(R4)2, - NHCOOR4, -NHS02N(R4)2, -CH2OR4, -CH2N(R4)2, -CH2NHC(0)R4, -S02 N(R4)2, - C(0)NHC(=NH)N(R4)2, -NHS02OR4, or CY, wherein CY is an optionally substituted group selected from a 3- to -7-membered cycloaliphatic; a 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; a 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein:
each R4 is independently selected from hydrogen, -OH, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
R4 is -Z2-R6 wherein:
Z2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-,
-S(0)2-, -C(O)-, -CO2-, -C(0)NR4a-, -C(NH)-, or -S(0)2NR4a-,
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen, -NH2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R , taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R2 is an optionally substituted group selected from 3- to 10-membered cycloaliphatic, 4- to 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R2 is optionally substituted with 1 -4 occurrences of R2a, wherein each occurrence of R2a is independently -R12a, -T2-R12d, -T2-R12a, or -V2-T2-R12d, and:
each occurrence of R12a is independently halogen, -CN, -N02, -R12c, -N(R12b)2,
-OR12b, -SR12c, -S(0)2R12c, -C(0)R12b, -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2,
-OC(0)N(R12b)2, -N(R12e)C(0)R12b, -N(R12e)S02R12c, -N(R12e)C(0)OR12b,
-N(R12e)C(0)N(R12b)2, or -N(R12e)S02N(R12b)2, or an optionally substituted d_6 aliphatic or d_6 haloaliphatic;
each occurrence of R12b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
each occurrence of R12c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, Ci_6 haloaliphatic, 3- to 10-membered cycloaliphatic, 4- to 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12d is independently hydrogen or an optionally substituted group selected from 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12e is independently hydrogen or an optionally substituted
Ci_6 aliphatic group; each occurrence of V2 is independently -N(R12e)-, -0-, -S-, -S(0)-,
-S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R12e)-, -S(0)2N(R12e)-, -OC(0)N(R12e)-,
-N(R12e)C(0)-, -N(R12e)S02-, -N(R12e)C(0)0-, -N(R12e)C(0)N(R12e)-,
-N(R12e)S02N(R12e)-, -OC(O)-, or -C(0)N(R12e)-0-; and
T2 is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R13)-, -0-, -S-, -S(0)-, -S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R13)-,
-S(0)2N(R13)-, -OC(0)N(R13)-, -N(R13)C(0)-, -N(R13)S02-, -N(R13)C(0)0-, -N(R13)C(0)N(R13)-, - N(R13)S(0)2N(R13)-, -OC(O)-, or -C(0)N(R13)-0- or wherein T2 or a portion thereof optionally forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocyclyl ring, wherein R13 is hydrogen or an optionally substituted Ci_4 aliphatic group; and
HY is a group selected from:
Figure imgf000073_0001
E , or F;
wherein
each occurrence of ¾, X5, X6, X7, and X8 is independently -CR10, -CR10 , or N, provided no more than two occurrences of X4, X5, X6, X7, and X8 is N;
each occurrence of Yi, Y2, Y3, Y4, Y5, Y6, Y7, and Y8 is -CR10;
each occurrence of Qi and Q2 is independently S, O or -NR9;
two adjacent occurrences of X4 and X5, Xe and X7, X7 and Xg, Yi and -NR9, Y3 and - NR9, or Y4 and Y5, may be taken together with the atoms to which they are bound, to form an unsubstituted fused heteroaryl or heterocyclyl group having 8 to 10 ring atoms and having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10 or R10' is independently -R10b, -V R10c, -T R10b, or -Vi-T R10b, wherein:
Vi is -NR11-, -NRu-C(0)-, -NRU-C(S)-, -NRU-C(NRU)-, -NRuC(0)0-, - NRuC(0)NRu-, -NRuC(0)S-, -NRuC(S)0-, -NRUC(S)NRU-, -NRUC(S)S-, - NRuC(NRu)0-, -NR11 CfNR1 ^NR11 -, -NRuS(0)2-, -NRuS(0)2NRu-, -C(O)-, -C02-, - C(0)NRu-, -C(0)NRuO-, -S02-, or -S02NRu-; each occurrence of R a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
Ti is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(RU)-, -0-, -S-, -S(O)-, -S(0)2-, -C(0)-, -C(0)0-, - C(0)N(Ru)-, -S(0)2N(Ru)-, -OC(0)N(Ru)-, -N(Ru)C(0)-, -N(Ru)S02-, - N(Rlla)C(0)0-, -N(R10a)C(O)N(R10a)-, -N(R10a)S(O)2N(R10a)-, -OC(O)-, or -C(0)N(Ru)- O- or wherein Ti forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocyclyl ring;
each occurrence of R10b is independently hydrogen, halogen, -CN, -N02, -N(RU)2, -OR10a, -SR10a, -S(O)2R10a, -C(O)R10a, -C(O)OR10a, -C(0)N(Ru)2,
-S(0)2N(Ru)2, -OC(0)N(Ru)2, -N(Ru)C(O)R10a, -N(Ru)SO2R10a, -N(Ru)C(O)OR10a, - N(Ru)C(0)N(Ru)2, or -N(Ru)S02N(Ru)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
R10a and R1<¾ taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R11 is independently hydrogen, -C(0)Rl la, -C02Rl la , -C(0)N(Rl la)2,
-C(0)N(Rlla)-ORlla, -S02Rl la, -S02N(Rlla)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of Rl la is independently hydrogen or an optionally substituted group selected from C e aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R9 is independently hydrogen, -C(0)R9a, -C02R9a, -C(0)N(R9b)2, -S02R9a, - S02N(R9b)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R% taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3- to 6-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and provided that when HY is a non- fused group then HY is substituted with at least one occurrence of R10 or R10', wherein R10 or R10' is:
-N(Ru)C(O)R10a, -C(0)N(Ru)2, or -NRuC(O)OR10a; or
-Vi-T R10b, wherein Vi is -NR11-, Ti is a Ci -C3 alkylene chain, and R is an optionally substituted 6- to 10-membered aryl ring or a 5- to 10-membered heteroaryl ring having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or Vi is -NRuC(0)NRu- , Ti is a Ci -C3 alkylene chain, and R10b is -OR10a; or
-Vi-R10c, wherein Vi is -NR11-, and R10c is a 5- to 10-membered heteroaryl ring having 1 - 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and
wherein a substituent on HY and R14, taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
provided that:
a) when R3 is hydrogen, then R1 is not
Figure imgf000076_0001
, wherein R103 is hydrogen or -C(0)2tBu; b) when R3 is hydrogen, then R1 is not -CH2OCH2CH2SiMe3;
c) when HY is a substituted thiazolyl ring, then R1 is not a substituted pyrrolidinyl ring;
HY is not substituted with
Figure imgf000076_0002
Figure imgf000076_0003
when HY is neither R1 nor R2 is a cyclopropyl ring; and
provided that the compound is other than:
Figure imgf000076_0004
DETAILED DESCRIPTION OF THE INVENTION
2. Compounds and Definitions: [0020] Compounds of this invention include those described generally for formula IA or VA, above, and are further illustrated by the classes, subclasses, and species disclosed herein. It will be appreciated that preferred subsets described for each variable herein can be used for any of the structural subsets as well. As used herein, the following definitions shall apply unless otherwise indicated.
[0021] As described herein, compounds of the invention may be optionally substituted with one or more substituents, such as are illustrated generally above, or as exemplified by particular classes, subclasses, and species of the invention. It will be appreciated that the phrase "optionally substituted" is used interchangeably with the phrase "substituted or unsubstituted." In general, the term "substituted", whether preceded by the term "optionally" or not, means that a hydrogen radical of the designated moiety is replaced with the radical of a specified substituent, provided that the substitution results in a stable or chemically feasible compound. The term "substitutable", when used in reference to a designated atom, means that attached to the atom is a hydrogen radical, which hydrogen atom can be replaced with the radical of a suitable substituent. Unless otherwise indicated, an "optionally substituted" group may have a substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position. Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds.
[0022] A stable compound or chemically feasible compound is one in which the chemical structure is not substantially altered when kept at a temperature from about -80°C to about +40°, in the absence of moisture or other chemically reactive conditions, for at least a week, or a compound which maintains its integrity long enough to be useful for therapeutic or prophylactic administration to a patient.
[0023] The phrase "one or more substituents", as used herein, refers to a number of substituents that equals from one to the maximum number of substituents possible based on the number of available bonding sites, provided that the above conditions of stability and chemical feasibility are met.
[0024] As used herein, the term "independently selected" means that the same or different values may be selected for multiple instances of a given variable in a single compound.
[0025] As used herein, "a 3-7-membered saturated, partially unsaturated, or aromatic monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-10- membered partially unsaturated, or aromatic bicyclic ring system having 0-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur" includes cycloaliphatic, heterocyclic, aryl and heteroaryl rings.
[0026] As used herein, the term "aromatic" includes aryl and heteroaryl groups as described generally below and herein. [0027] The term "aliphatic" or "aliphatic group", as used herein, means an optionally substituted straight-chain or branched Ci_n hydrocarbon, or a cyclic hydrocarbon which is completely saturated or which contains one or more units of unsaturation, but which is not aromatic (also referred to herein as "carbocycle", "cycloaliphatic", "cycloalkyl", or "cycloalkenyl"). For example, suitable aliphatic groups include optionally substituted linear, branched or cyclic alkyl, alkenyl, alkynyl groups and hybrids thereof, such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl, or (cycloalkyl)alkenyl. Unless otherwise specified, in various embodiments, aliphatic groups have 1-12, 1-10, 1-8, 1-6, 1-4, 1-3, or 1-2 carbon atoms.
[0028] The term "alkyl", used alone or as part of a larger moiety, refers to an optionally substituted straight or branched chain hydrocarbon group having 1-12, 1-10, 1-8, 1-6, 1-4, 1-3, or 1-2 carbon atoms.
[0029] The term "alkenyl", used alone or as part of a larger moiety, refers to an optionally substituted straight or branched chain hydrocarbon group having at least one double bond and having 2- 12, 2-10, 2-8, 2-6, 2-4, or 2-3 carbon atoms.
[0030] The term "alkynyl", used alone or as part of a larger moiety, refers to an optionally substituted straight or branched chain hydrocarbon group having at least one triple bond and having 2-12, 2-10, 2-8, 2-6, 2-4, or 2-3 carbon atoms.
[0031] The terms "cycloaliphatic", "carbocycle", "carbocyclyl", "carbocyclo", or "carbocyclic", used alone or as part of a larger moiety, refer to an optionally substituted saturated or partially unsaturated cyclic aliphatic ring system having from 3 to about 14 ring carbon atoms. In some embodiments, the cycloaliphatic group is an optionally substituted monocyclic hydrocarbon having 3-8 or 3-6 ring carbon atoms. Cycloaliphatic groups include, without limitation, optionally substituted cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptyl, cycloheptenyl, cyclooctyl, cyclooctenyl, or cyclooctadienyl. The terms "cycloaliphatic", "carbocycle", "carbocyclyl", "carbocyclo", or "carbocyclic" also include optionally substituted bridged or fused bicyclic rings having 6-12, 6- 10, or 6-8 ring carbon atoms, wherein any individual ring in the bicyclic system has 3-8 ring carbon atoms.
[0032] The term "cycloalkyl" refers to an optionally substituted saturated ring system of about 3 to about 10 ring carbon atoms. Exemplary monocyclic cycloalkyl rings include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cycloheptyl.
[0033] The term "cycloalkenyl" refers to an optionally substituted non-aromatic monocyclic or multicyclic ring system containing at least one carbon-carbon double bond and having about 3 to about 10 carbon atoms. Exemplary monocyclic cycloalkenyl rings include cyclopentyl, cyclohexenyl, and cycloheptenyl.
[0034] The terms "haloaliphatic", "haloalkyl", "haloalkenyl" and "haloalkoxy" refer to an aliphatic, alkyl, alkenyl or alkoxy group, as the case may be, which is substituted with one or more halogen atoms. As used herein, the term "halogen" or "halo" means F, CI, Br, or I. The term "fluoroaliphatic" refers to a haloaliphatic wherein the halogen is fluoro, including perfluorinated aliphatic groups. Examples of fluoroaliphatic groups include, without limitation, fluoromethyl, difluoromethyl, trifluoromethyl, 2- fluoroethyl, 2,2,2-trifluoroethyl, 1 , 1 ,2-trifluoroethyl, 1 ,2,2-trifluoroethyl, and pentafluoroethyl.
[0035] The term "heteroatom" refers to one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3,4-dihydro- 2H-pyrrolyl), NH (as in pyrrolidinyl) or NR+ (as in N-substituted pyrrolidinyl)).
[0036] The terms "aryl" and "ar-", used alone or as part of a larger moiety, e.g., "aralkyl", "aralkoxy", or "aryloxyalkyl", refer to an optionally substituted C6-i4aromatic hydrocarbon moiety comprising one to three aromatic rings. Preferably, the aryl group is a C6-ioaryl group. Aryl groups include, without limitation, optionally substituted phenyl, naphthyl, or anthracenyl. The terms "aryl" and "ar-", as used herein, also include groups in which an aryl ring is fused to one or more cycloaliphatic rings to form an optionally substituted cyclic structure such as a tetrahydronaphthyl, indenyl, or indanyl ring. The term "aryl" may be used interchangeably with the terms "aryl group", "aryl ring", and "aromatic ring".
[0037] An "aralkyl" or "arylalkyl" group comprises an aryl group covalently attached to an alkyl group, either of which independently is optionally substituted. Preferably, the aralkyl group is C6-io arylCi_6alkyl, including, without limitation, benzyl, phenethyl, and naphthylmethyl.
[0038] The terms "heteroaryl" and "heteroar-", used alone or as part of a larger moiety, e.g., "heteroaralkyl", or "heteroaralkoxy", refer to groups having 5 to 14 ring atoms, preferably 5, 6, 9, or 10 ring atoms; having 6, 10, or 14 π electrons shared in a cyclic array; and having, in addition to carbon atoms, from one to five heteroatoms. A heteroaryl group may be mono-, bi-, tri-, or polycyclic, preferably mono-, bi-, or tricyclic, more preferably mono- or bicyclic. The term "heteroatom" refers to nitrogen, oxygen, or sulfur, and includes any oxidized form of nitrogen or sulfur, and any quaternized form of a basic nitrogen. For example, a nitrogen atom of a heteroaryl may be a basic nitrogen atom and may also be optionally oxidized to the corresponding N-oxide. When a heteroaryl is substituted by a hydroxy group, it also includes its corresponding tautomer. The terms "heteroaryl" and "heteroar-", as used herein, also include groups in which a heteroaromatic ring is fused to one or more aryl, cycloaliphatic, or heterocycloaliphatic rings. Nonlimiting examples of heteroaryl groups include thienyl, furanyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolizinyl, purinyl, naphthyridinyl, pteridinyl, indolyl, isoindolyl, benzothienyl, benzofuranyl, dibenzofuranyl, indazolyl, benzimidazolyl, benzthiazolyl, quinolyl, isoquinolyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, 4H-quinolizinyl, carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, and pyrido[2,3-b]-l,4-oxazin-3(4H)-one. The term "heteroaryl" may be used interchangeably with the terms "heteroaryl ring", "heteroaryl group", or "heteroaromatic", any of which terms include rings that are optionally substituted. The term "heteroaralkyl" refers to an alkyl group substituted by a heteroaryl, wherein the alkyl and heteroaryl portions independently are optionally substituted.
[0039] As used herein, the terms "heterocycle", "heterocyclyl", "heterocyclic radical", and "heterocyclic ring" are used interchangeably and refer to a stable 3- to 8-membered monocyclic or 7-10- membered bicyclic heterocyclic moiety that is either saturated or partially unsaturated, and having, in addition to carbon atoms, one or more, preferably one to four, heteroatoms, as defined above. When used in reference to a ring atom of a heterocycle, the term "nitrogen" includes a substituted nitrogen. As an example, in a saturated or partially unsaturated ring having 0-3 heteroatoms selected from oxygen, sulfur or nitrogen, the nitrogen may be N (as in 3,4-dihydro-2H-pyrrolyl), NH (as in pyrrolidinyl), or NR+ (as in N-substituted pyrrolidinyl).
[0040] A heterocyclic ring can be attached to its pendant group at any heteroatom or carbon atom that results in a stable structure and any of the ring atoms can be optionally substituted. Examples of such saturated or partially unsaturated heterocyclic radicals include, without limitation, tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, decahydroquinolinyl, oxazolidinyl, piperazinyl, dioxanyl, dioxolanyl, diazepinyl, oxazepinyl, thiazepinyl, morpholinyl, and thiamorpholinyl. A heterocyclyl group may be mono-, bi-, tri-, or polycyclic, preferably mono-, bi-, or tricyclic, more preferably mono- or bicyclic. The term "heterocyclylalkyl" refers to an alkyl group substituted by a heterocyclyl, wherein the alkyl and heterocyclyl portions independently are optionally substituted. Additionally, a heterocyclic ring also includes groups in which the heterocyclic ring is fused to one or more aryl rings.
[0041] As used herein, the term "partially unsaturated" refers to a ring moiety that includes at least one double or triple bond between ring atoms. The term "partially unsaturated" is intended to encompass rings having multiple sites of unsaturation, but is not intended to include aromatic (e.g., aryl or heteroaryl) moieties, as herein defined.
[0042] The term "alkylene" refers to a bivalent alkyl group. An "alkylene chain" is a polymethylene group, i.e., -(CH2)n-, wherein n is a positive integer, preferably from 1 to 6, from 1 to 4, from 1 to 3, from 1 to 2, or from 2 to 3. An optionally substituted alkylene chain is a polymethylene group in which one or more methylene hydrogen atoms is optionally replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group and also include those described in the specification herein. It will be appreciated that two substituents of the alkylene group may be taken together to form a ring system. In certain embodiments, two substituents can be taken together to form a 3-7-membered ring. The substituents can be on the same or different atoms. [0043] An alkylene chain also can be optionally interrupted by a functional group. An alkylene chain is "interrupted" by a functional group when an internal methylene unit is interrupted by the functional group. Examples of suitable "interrupting functional groups" are described in the specification and claims herein.
[0044] For purposes of clarity, all bivalent groups described herein, including, e.g., the alkylene chain linkers described above, are intended to be read from left to right, with a corresponding left-to-right reading of the formula or structure in which the variable appears.
[0045] An aryl (including aralkyl, aralkoxy, aryloxyalkyl and the like) or heteroaryl (including heteroaralkyl and heteroarylalkoxy and the like) group may contain one or more substituents and thus may be "optionally substituted". In addition to the substituents defined above and herein, suitable substituents on the unsaturated carbon atom of an aryl or heteroaryl group also include and are generally selected from -halo, -N02, -CN, -R+, -C(R+)=C(R+)2, -C≡C-R+, -OR+, -SR°, -S(0)R°, -S02R°, -S03R+, -S02N(R+)2, -N(R+)2, -NR+C(0)R+, -NR+C(S)R+, -NR+C(0)N(R+)2, -NR+C(S)N(R+)2, -N(R+)C(=NR+)-N(R+)2, -N(R+)C(=NR+)-R°, -NR+C02R+, -NR+S02R°, -NR+S02N(R+)2, -0-C(0)R+, -0-C02R+, -OC(0)N(R+)2, -C(0)R+, -C(S)R°, -C02R+, -C(0)-C(0)R+, -C(0)N(R+)2, -C(S)N(R+)2, -C(0)N(R+)-OR+, -C(0)N(R+)C(=NR+)-N(R+)2, -N(R+)C(=NR+)-N(R+)-C(0)R+, -C(=NR+)-N(R+)2, -C(=NR+)-OR+, -N(R+)-N(R+)2, -C(=NR+)-N(R+)-OR+, -C(R°)=N-OR+, -P(0)(R+)2, -P(0)(OR+)2, -0-P(0)-OR+, and -P(0)(NR+)-N(R+)2, wherein R+, independently, is hydrogen or an optionally substituted aliphatic, aryl, heteroaryl, cycloaliphatic, or heterocyclyl group, or two independent occurrences of R+ are taken together with their intervening atom(s) to form an optionally substituted 5-7-membered aryl, heteroaryl, cycloaliphatic, or heterocyclyl ring. Each R° is an optionally substituted aliphatic, aryl, heteroaryl, cycloaliphatic, or heterocyclyl group.
[0046] An aliphatic or heteroaliphatic group, or a non-aromatic carbocyclic or heterocyclic ring may contain one or more substituents and thus may be "optionally substituted". Unless otherwise defined above and herein, suitable substituents on the saturated carbon of an aliphatic or heteroaliphatic group, or of a non-aromatic carbocyclic or heterocyclic ring are selected from those listed above for the unsaturated carbon of an aryl or heteroaryl group and additionally include the following: =0, =S, =C(R*)2, =N- N(R*)2, =N-OR*, =N-NHC(0)R*, =N-NHC02R° =N-NHS02R° or =N-R* where R° is defined above, and each R* is independently selected from hydrogen or an optionally substituted Ci_6 aliphatic group.
[0047] In addition to the substituents defined above and herein, optional substituents on the nitrogen of a non-aromatic heterocyclic ring also include and are generally selected from -R+, -N(R+)2, -C(0)R+, -C(0)OR+, -C(0)C(0)R+, -C(0)CH2C(0)R+, -S(0)2R+, -S(0)2N(R+)2, -C(S)N(R+)2, -C(=NH)-N(R+)2, or -N(R+)S(0)2R+; wherein each R+ is defined above. A ring nitrogen atom of a heteroaryl or non-aromatic heterocyclic ring also may be oxidized to form the corresponding N-hydroxy or N-oxide compound. A nonlimiting example of such a heteroaryl having an oxidized ring nitrogen atom is N-oxidopyridyl.
[0048] As detailed above, in some embodiments, two independent occurrences of R+ (or any other variable similarly defined in the specification and claims herein), are taken together with their intervening atom(s) to form a monocyclic or bicyclic ring selected from 3-13-membered cycloaliphatic, 3-12-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0049] Exemplary rings that are formed when two independent occurrences of R+ (or any other variable similarly defined in the specification and claims herein), are taken together with their intervening atom(s) include, but are not limited to the following: a) two independent occurrences of R+ (or any other variable similarly defined in the specification or claims herein) that are bound to the same atom and are taken together with that atom to form a ring, for example, N(R+)2, where both occurrences of R+ are taken together with the nitrogen atom to form a piperidin- 1 -yl, piperazin- 1 -yl, or morpholin-4-yl group; and b) two independent occurrences of R+ (or any other variable similarly defined in the specification or claims herein) that are bound to different atoms and are taken together with both of those atoms to form a ring,
for example where a phenyl group is substituted with two occurrences of OR+
Figure imgf000082_0001
, these two occurrences of R+ are taken together with the oxygen atoms to which they are bound to form a fused 6-
membered oxygen containing ring:
Figure imgf000082_0002
It will be appreciated that a variety of other rings
(e.g., spiro and bridged rings) can be formed when two independent occurrences of R+ (or any other variable similarly defined in the specification and claims herein) are taken together with their intervening atom(s) and that the examples detailed above are not intended to be limiting.
[0050] Exemplary rings that are formed when two independent occurrences of ¾ and X5, Xe and X7, or X7 and X8; are taken together with their intervening atom(s) to form a fused group having 8 to 10 ring atoms inclu ited to the following:
Figure imgf000082_0003
Figure imgf000083_0001
[0051] Exemplary rings that are formed when two independent occurrences of Yi and -NR9, Y3 and -NR9, Y4 and Y5, or Y6 and Y7 are taken together with their intervening atom(s) to form a fused group having 8 to 10 rin atoms include, but are not limited to the following:
Figure imgf000084_0001
[0052] Unless otherwise stated, structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, and geometric (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, (Z) and (E) double bond isomers, and (Z) and (E) conformational isomers. Therefore, single stereochemical isomers as well as enantiomeric, diastereomeric, and geometric (or conformational) mixtures of the present compounds are within the scope of the invention. Unless otherwise stated, all tautomeric forms of the compounds of the invention are within the scope of the invention. Additionally, unless otherwise stated, structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms. For example, compounds having the present structures where there is a replacement of hydrogen by deuterium or tritium, or a replacement of a carbon by a 13C- or 14C-enriched carbon are within the scope of this invention. Such compounds are useful, as a nonlimiting example, as analytical tools or probes in biological assays.
[0053] It is to be understood that, when a disclosed compound has at least one chiral center, the present invention encompasses one enantiomer of inhibitor free from the corresponding optical isomer, racemic mixture of the inhibitor and mixtures enriched in one enantiomer relative to its corresponding optical isomer. When a mixture is enriched in one enantiomer relative to its optical isomers, the mixture contains, for example, an enantiomeric excess of at least 50%, 75%, 90%, 95% 99% or 99.5%. [0054] The enantiomers of the present invention may be resolved by methods known to those skilled in the art, for example by formation of diastereoisomenc salts which may be separated, for example, by crystallization; formation of diastereoisomeric derivatives or complexes which may be separated, for example, by crystallization, gas-liquid or liquid chromatography; selective reaction of one enantiomer with an enantiomer-specific reagent, for example enzymatic esterification; or gas-liquid or liquid chromatography in a chiral environment, for example on a chiral support for example silica with a bound chiral ligand or in the presence of a chiral solvent. Where the desired enantiomer is converted into another chemical entity by one of the separation procedures described above, a further step is required to liberate the desired enantiomeric form. Alternatively, specific enantiomers may be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents, or by converting one enantiomer into the other by asymmetric transformation.
[0055] When a disclosed compound has at least two chiral centers, the present invention encompasses a diastereomer free of other diastereomers, a pair of diastereomers free from other diasteromeric pairs, mixtures of diasteromers, mixtures of diasteromeric pairs, mixtures of diasteromers in which one diastereomer is enriched relative to the other diastereomer(s) and mixtures of diasteromeric pairs in which one diastereomeric pair is enriched relative to the other diastereomeric pair(s). When a mixture is enriched in one diastereomer or diastereomeric pair(s) relative to the other diastereomers or diastereomeric pair(s), the mixture is enriched with the depicted or referenced diastereomer or diastereomeric pair(s) relative to other diastereomers or diastereomeric pair(s) for the compound, for example, by a molar excess of at least 50%, 75%, 90%, 95%, 99% or 99.5%.
[0056] The diastereoisomeric pairs may be separated by methods known to those skilled in the art, for example chromatography or crystallization and the individual enantiomers within each pair may be separated as described above. Specific procedures for chromatographically separating diastereomeric pairs of precursors used in the preparation of compounds disclosed herein are provided the examples herein.
[0057] 3. Description of Exemplary Compounds:
[0058] Other embodiments of the invention relate to a subgenus of the compounds of formula IA, characterized by formula IIA:
Figure imgf000085_0001
IIA
Larmaceutically acceptable salt thereof, where variables HY, R1, R2, and R3 are as defined above for formula IA.
[0059] Other embodiments of the invention relate to a subgenus of the compounds of formula IA, characterized by formula IIIA:
Figure imgf000086_0001
IIIA
or a pharmaceutically acceptable salt thereof, where variables HY, R1, R2, and R3 are as defined above for formula IA.
[0060] Other embodiments of the invention relate to a subgenus of the compounds of formula IA, characterized by formula IVA:
Figure imgf000086_0002
IVA
or a pharmaceutically acceptable salt thereof, where variables HY, R1, R2, and R3 are as defined above for formula IA.
[0061] Other embodiments of the invention relate to a subgenus of the compounds of formula IA, characterized by formula VA:
Figure imgf000086_0003
VA
or a pharmaceutically acceptable salt thereof, where variables HY, R1, R2, and R3 are as defined above for formula IA.
[0062] In certain embodiments, for compounds of general formula IA, IIA, IIIA, IVA, or VA, R1 is
Figure imgf000087_0001
CY and CY is 1 or 11 ; wherein:
Xi, X2, and X3, are each independently N, O, S, NR4 , or CR7, provided that only one of
Xi, X2, or X3 may be O or S;
Y9 is nitrogen or carbon;
Gi4 is CR7', -N= or -NR4'-, wherein:
R4 is independently hydrogen, -Z2-R6, optionally substituted
Ci_6 aliphatic, or optionally substituted 3-10-membered cycloaliphatic, wherein:
Z2 is selected from an optionally substituted Ci_3 alkylene chain, -S(O)-, -S(0)2-, -C(O)-, -C02-, -C(0)NR4a-, or -S(0)2NR4\
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is an optionally substituted group selected from d_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R7 and R7 is independently hydrogen, -CN, halogen, -Z3-R8, Ci_6 aliphatic, or 3-10-membered cycloaliphatic, wherein:
Z3 is selected from an optionally substituted Ci_3 alkylene chain, -0-, -N(R7a)-, - S-, -S(O)-, -S(0)2-, -C(O)-, -C02-, -C(0)NR7\ -N(R7a)C(0)-, -N(R7a)C02-, -S(0)2NR7a-, -N(R7a)S(0)2-, -OC(0)N(R7a)-, -N(R7a)C(0)NR7a-, -N(R7a)S(0)2N(R7a)-, or -OC(O)-;
R7a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R8 is an optionally substituted group selected from d_6 aliphatic, 3-10-membered cycloaliphatic, 4-10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0063] In certain embodiments, for compounds of general formula IA, IIA, IIIA, IV A, or VA, R1 is
Figure imgf000087_0002
CY and CY is 1 or 11 ; wherein: Xi, X2, and X3, are each independently N, O, S, NR4 , or CR7, provided that only one of
Xi, X2, or X3 may be O or S;
Y9 is nitrogen or carbon;
Gi4 is CR7', -N= or -NR4'-, wherein:
R4 is independently hydrogen, -Z2-R6, optionally substituted
Ci_6 aliphatic, or optionally substituted 3- 10-membered cycloaliphatic, wherein:
Z2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-, -S(0)2-, -C(O)-, -CO2-, -C(0)NR4a-, or -S(0)2NR4a-,
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is an optionally substituted group selected from d_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R7 and R7 is independently hydrogen, -CN, halogen, -NH2, -Z3-R8, Ci_6 aliphatic, or 3-10-membered cycloaliphatic, wherein:
Z3 is selected from an optionally substituted Q_3 alkylene chain, -0-, -N(R7a)-, - S-, -S(O)-, -S(0)2-, -C(O)-, -CO2-, -C(0)NR7a-, -N(R7a)C(0)-, -N(R7a)C02-, -S(0)2NR7a-, -N(R7a)S(0)2-, -OC(0)N(R7a)-, -N(R7a)C(0)NR7a-, -N(R7a)S(0)2N(R7a)-, or -OC(O)-;
R7a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R8 is hydrogen or an optionally substituted group selected from d_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
Figure imgf000088_0001
[0064] In other embodiments, for compounds described directly above, CY is 1
[0065] In some embodiments for compounds of formula IA, IIA, IIIA, IVA, or VA, Y9 is carbon,
Xi is nitrogen, G14 is N(R4 ), and X2 and X3, are CH.
[0066] In yet other embodiments, Y9 is carbon, Xi and X3 are nitrogen, G14 is N(R4 ), and X2 is CH.
[0067] In other embodiments, Y9 is carbon, Xi and G14 are nitrogen, X3 is N(R4 ), and X2 is CH.
[0068] In other embodiments, Y9 is carbon, Xi and X2 are nitrogen, G14 is N(R4 ), and X3 is CH.
[0069] In other embodiments, Y9 is carbon, G14 is N(R4 ), X3 is nitrogen, and Xi and X2 are CH. [0070] In other embodiments, Y9 is carbon, G14 is nitrogen, X3 is N(R4 ), and Xi and X2 are CH.
[0071] In other embodiments, Y9 is carbon, X3 is nitrogen, X2 is N(R4 ), and Xi and G14 are CH.
[0072] In other embodiments, Y9 is carbon, X2 is nitrogen, G14 is N(R4 ), and Xi and X3, are CH.
[0073] In other embodiments, Y9 is carbon, X2 is N(R4 ), G14 is nitrogen, and Xi and X3, are CH.
[0074] In still other embodiments, for compounds of general formula IA, IIA, IIIA, IVA, or VA, R is Cy and Cy is an optionally substituted 6-membered aryl or heteroaryl ring.
[0075] In still other embodiments, R1 is Cy and Cy is an optionally substituted 5- to 6-membered heteroaryl or heterocyclyl ring.
[0076] In yet other embodiments, R1 is Cy and Cy is selected from:
Figure imgf000089_0001
wherein R1 is optionally further substituted with one or more occurrences of R7 or R4'.
[0077] In other embodiments, R1 is Cy, and Cy is selected from:
Figure imgf000089_0002
Figure imgf000090_0001
wherein Cy is optionally further substituted with one or more occurrences ofR7 or R4'.
0078 In other embodiments 1 is C and C is selected from:
Figure imgf000090_0002
wherein 1 is optionally further substituted with one or more occurrences of R7 or R4'.
R1 is Cy, and Cy is selected from:
Figure imgf000090_0003
wherein R1 is optionally further substituted with one or more occurrences of R7 or R4'.
[0080] In other embodiments, R1 is Cy, and Cy is an optionally substituted 6-membered aryl ring.
[0081] In other embodiments, R1 is -CON(R4)2, -C(0)OR4, -NHCOR4, or CH2OR4.
[0082] In any of the embodiments described above for R1, other variables HY, R2, R3, R10, R10', R10a,
R7, R4', and R14 are as defined in any one of the embodiments described herein.
[0083] In some embodiments for compounds of general formula IA, IIA, IIIA, IVA, or VA, HY is selected from:
Figure imgf000090_0004
H J P
Figure imgf000091_0001
L M Z
where variables X5, X6, X7, Qi, Q2, Yi, R10, R10a, and R11 are as defined herein.
[0084] In some embodiments for compounds of general formula IA, IIA, IIIA, IVA, or VA, HY is selected from:
Figure imgf000091_0002
wherein each occurrence of X5, Χβ, and X7 is independently -CR10 , -CR10 or N, provided no more than two occurrences of X5, Χβ, and X7 are N;
each occurrence of Qi and Q2 is independently S, O or -NR9;
each occurrence of Yi and Y7 is independently -CR10;
or wherein two adjacent occurrences of X6, and X7, Yi and -NR9, or two adjacent occurrences of R10 taken together with the atoms to which they are bound, form an unsubstituted fused heteroaryl or heterocyclyl group having 8 to 10 ring atoms and having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur. [0085] In yet other embodiments, HY is selected from:
Figure imgf000092_0001
H J
Figure imgf000092_0002
P
[0086] In some embodiments for compounds of general formula IA, IIA, IIIA, IVA, or VA, HY is selected from:
Figure imgf000092_0003
L M Z
where variables X5, X6, X7, Qi, Q2, Yi, R10, R10a, and R11 are as defined herein.
[0087] In some other embodiments for compounds of general formula IA, IIA, IIIA, IVA, or VA, HY is selected from:
Figure imgf000092_0004
Figure imgf000093_0001
Figure imgf000093_0002
Figure imgf000093_0003
wherein each fused HY group is unsubstituted, and
each non- fused HY group is substituted with one or more occurrences of R10 or R10 , and at least one occurrence of R10 or R10' is -N(Ru)C(O)R10a, -N(Ru)C(O)OR10a, or -C(0)N(Ru)2, and the dashed line represents a single bond or a double bond.
[0088] In some embodiments for compounds of general formula IA, IIA, IIIA, IVA, or VA, R10a is Ci_6 aliphatic substituted with a 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0089] In still other embodiments, HY is selected from:
Figure imgf000094_0001
Figure imgf000094_0002
wherein each fused HY group is unsubstituted, and each non- fused HY group is substituted with one or more occurrences of R10 or R10 , and at least one occurrence of R10 or R10 is -N(Ru)C(O)R10a, - N(Ru)C(O)OR10a, or -C(0)N(Ru)2, and the dashed line represents a single bond or a double bond.
[0090] In yet other embodiments, HY is
Figure imgf000094_0003
, wherein HY is substituted with one or more occurrences of R10 or R10 .
[0091] In still other embodiments, HY is
Figure imgf000094_0004
, wherein R10 is hydrogen, methyl, chloro, bromo, fluoro, CN, CF3, OR10a, COR10a, and R10 is NHCOR10a or -NHC(O)OR10a.
[0092] In still other embodiments for compounds of general formula IA, IIA, IIIA, IV A, or VA, R10 is hydrogen, methyl, or chloro, and R10 is -NHCOR10a or -NHCOOR10a.
[0093] In yet other embodiments, R10 is hydrogen, methyl, or chloro, and R10 is -NHR11, wherein R11 is an optionally substituted group selected from 6- 10-membered aryl, or 5-10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0094] In yet other embodiments, R10 is hydrogen, methyl, or chloro.
[0095] In still other embodiments, R10' is methyl, and R10 is -NHCOR10a. [0096] In still other embodiments, R10 is -NHR11, wherein R11 is an optionally substituted 5- to 10- membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0097] In other embodiments, R10a is cyclopropyl, methyl, ethyl, or isopropyl.
[0098] In any of the embodiments described above for HY, R10, R10', or R10a, other variables R1, R2, R3, and R14 are as defined in any one of the embodiments described herein.
[0099] In some embodiments for compounds of formula IA, IIA, IIIA, IVA, or VA, R2 is a 6- 10- membered aryl or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; optionally substituted with 1 -3 occurrences of R2a.
[00100] In other embodiments, R2 is a phenyl or pyridyl group;
[00101] In other embodiments, R2 is a phenyl group; optionally substituted with 1 to 4 independent occurrences of halogen, Ci_3 alkyl, -CN, Ci_3 haloalkyl, -(CH2)pN(R12b)2, -OR12b, -NHC(0)R12b, - NHC(0)NHR12b, -NHS(0)2R12b, -S(0)2R12c, -S(0)2N(R12b)2, -C(0)OR12b, -C(0)N(R12b)2, or -C(0)R12b; wherein R12b and R12c are defined as described herein or
wherein two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0-1 additional heteroatoms selected from nitrogen, oxygen, or sulfur, and wherein p is 0 to 3.
[00102] In other embodiments, R2 is a phenyl group; optionally substituted with 1 to 4 independent occurrences of halogen, Ci_3 alkyl, -CN, Ci_3 haloalkyl, -(CH2)pN(R12b)2, -OR12b, -NHC(0)R12b, - NHC(0)NHR12b, -NHS(0)2R12b, -S(0)2R12c, -S(0)2N(R12b)2, C(0)OR12b, -C(0)N(R12b)2, or -C(0)R12b, and wherein p is 0 to 3.
[00103] In yet other embodiments, R2 is a phenyl group; optionally substituted with 1 to 4 independent occurrences of halogen, Ci_3 alkyl, -CN, Ci_3 haloalkyl, -CH2N(CH3)2, -OCi_3 alkyl, -OCi_3 haloalkyl, -SCi_3 haloalkyl, -NHC(0)Ci_3 alkyl, -NHC(0)NHCi_3 alkyl, -NHS(0)2Ci_3 alkyl, or -C(0)H.
[00104] In other embodiments, R2 is substituted with 1 or 2 independent occurrences of R2a. In some embodiments R2a is a halo or methyl group.
[00105] In yet other embodiments, R2 is a phenyl group substituted with 1 or 2 occurrences of halogen. In yet other embodiments, R2 is a phenyl group substituted with methyl.
[00106] In still other embodiments, R2 is a 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[00107] In yet other embodiments, R2 is an optionally substituted N-linked 3-, 4-, 5-, 6-, or 7- membered heterocyclyl ring, optionally substituted with one or more occurrences of R2a.
[00108] In still other embodiments, R2 is optionally substituted with one or more Q_3 alkyl groups, - [00109] In still other embodiments, R2 is a Ci_6 aliphatic and each occurrence of R2a is independently -
C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2, -N(R12e)C(0)R12b, or -N(R12e)S02R12c.
[00110] In still other embodiments, R2 is a Ci_6 aliphatic, optionally substituted with halo, -N(R12b)2, or a cyclopropyl ring, wherein each R12b is independently selected from hydrogen, methyl, or ethyl, or wherein two R12b, taken together with a nitrogen atom to which they are bound, form a pyrrolidinyl ring. In still other embodiments, R2 is a d_3 aliphatic.
[00111] In still other embodiments, R2 is halogen. In other embodiments, R2 is hydrogen.
[00112] In any of the embodiments described above for R2, other variables HY, R1, R3, R10, R10', R10a, and R14 are as defined in any one of the embodiments described herein.
[00113] In certain embodiments, for compounds of general formula IA, IIA, IIIA, IVA, or VA, R1 is CY, -CON(R4)2, -NHCOR4, or -COOR4; R2 is an optionally substituted 6- 10-membered aryl or 5-10- membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and HY is selected from
Figure imgf000096_0001
wherein each fused HY group is unsubstituted, and
each non-fused HY group is substituted with one or more occurrences of R10 or R10 , and at least one occurrence of R10 or R10' is -N(Ru)C(O)R10a or -C(0)N(Ru)2, and the dashed line represents a single bond or a double bond.
[00114] General Synthetic Methods and Intermediates: [00115] The compounds of the present invention can be prepared by methods known to one of ordinary skill in the art and / or by reference to the schemes shown below and the synthetic examples that follow. Exemplary synthetic routes are set forth in the Schemes below, and in the Examples.
[00116] Examples of the solvent for the below-mentioned reactions include, but are not limited to halogenated hydrocarbons such as dichloromethane, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like, aromatic hydrocarbons such as benzene, toluene, xylene and the like, alcohols such as methanol, ethanol, isopropanol, fert-butanol, phenol and the like, ethers such as diethyl ether, tetrahydrofuran, dioxane, DME and the like, acetone, ACN, ethyl acetate, NN-dimethylformamide, NN- dimethylacetamide, 1 -methyl-2-pyrrolidone, dimethyl sulfoxide, hexamethylphosphoramide, water or a mixed solvent thereof and the like.
[00117] One of ordinary skill in the art will recognize that numerous variations in reaction conditions including variations in solvent, reagents, catalysts, reaction temperatures and times are possible for each of the reactions described. Variation of order of synthetic steps and alternative synthetic routes are also possible.
[00118] In many cases, synthesis can be started from commercially available imidazole analogs to prepare target compounds. In some cases, specially functionalized imidazole analogs can be prepared by the procedures described in the Schemes below.
[ 119] Scheme 1: General method for the synthesis of imidazoles xv
Figure imgf000097_0001
xiii xiv xv
[00120] Scheme 1 describes a method of preparing substituted imidazoles xv. Treatment of methyl lH-imidazole-5-carboxylate (viii) with a brominating reagent such as NBS in an appropriate solvent gives ix. Protection of the imidazole nitrogen with a standard protecting group (PG) suCh as SEM gives compounds x. Compounds xi can then be prepared from compounds x byod A can subsequently be used to prepare compounds xii from xi when R2 is an aromatic or heteroaromatic group. When R2 is a substituted amino group, compounds xii can be prepared by Method B Compounds xiv can be prepared via the intermediate acids xiii (obtained by hydrolysis of the ester of compounds xii under standard conditions) or by transformation of the esters xii directly to a variety of groups using standard methods.
Removal of the protecting group under standard conditions can provide the desired ;midaz0les xv.
[00121] Scheme 2: General method for the synthesis of imidazoles xix
Figure imgf000098_0001
xvi xvii xviii xix
[00122] Scheme 2 describes a method of preparing substituted imidazoles xix. Treatment of 2,4- dibromo- lH-imidazole (xvi) with an aryl fluoride in the presence of a base such as potassium carbonate in a solvent, for example DMF, is a method that can be used to prepare compounds xvii. Compounds xviii can be prepared by treatment of compounds xvii according to Method A. Fully substituted imidazoles xix can then be obtained by again using Method A.
[00123] Scheme 3: General method for the synthesis of imidazoles xxv
Figure imgf000098_0002
[00124] Scheme 3 describes a method of preparing substituted imidazoles xxv. Protection of the imidazole (xx) nitrogen with a standard protecting group (PG) such as SEM gives compounds xxi. Compounds xxii can be prepared by treatment of compounds xxi according to Method A. Metal halogen exchange with a base such as «-BuLi in a solvent, for example THF, followed by quench with water can provide compounds xxiii from imidazoles xxii. Compounds xxiv can be prepared by treatment of compounds xxiii according to Method A. Fully substituted imidazoles xxv can be obtained from compounds xxiv by removal of the protecting group under standard conditions followed by treatment with an aryl fluoride in the presence of a base such as potassium carbonate in a solvent, for example DMF. Alternatively, following removal of the protecting group under standard conditions compounds xxv can be prepared according to Method B.
[00125] The compounds of the present invention can be prepared by methods known to one of ordinary skill in the art and / or by reference to the schemes shown below and the synthetic examples that follow.
[00126] 4. Uses, Formulation and Administration [00127] As discussed above, the present invention provides compounds that are useful as inhibitors of VPS34 and/or PI3K, and thus the present compounds are useful for treating proliferative, inflammatory, or cardiovascular disorders such as tumor and/or cancerous cell growth mediated by VPS34 and/or PI3K. In particular, the compounds are useful in the treatment of cancers in a subject, including, but not limited to, lung and bronchus, including non-small cell lung cancer (NSCLC), squamous lung cancer, brochioloalveolar carcinoma (BAC), adenocarcinoma of the lung, and small cell lung cancer (SCLC); prostate, including androgen-dependent and androgen-independent prostate cancer; breast, including metastatic breast cancer; pancreas; colon and rectum; thyroid; liver and intrahepatic bile duct; hepatocellular; gastric; endometrial; melanoma; kidney; and renal pelvis, urinary bladder; uterine corpus; uterine cervix; ovary, including progressive epithelial or primary peritoneal cancer; multiple myeloma; esophagus; acute myelogenous leukemia (AML); chronic myelogenous leukemia (CML), including accelerated CML and CML blast phase (CML-BP); lymphocytic leukemia; myeloid leukemia; acute lymphoblastic leukemia (ALL); chronic lymphocytic leukemia (CLL); Hodgkin's disease (HD); non- Hodgkin's lymphoma (NHL), including follicular lymphoma and mantle cell lymphoma; B-cell lymphoma, including diffuse large B-cell lymphoma (DLBCL); T-cell lymphoma; multiple myeloma (MM); amyloidosis; Waldenstrom's macroglobulinemia; myelodysplastic syndromes (MDS), including refractory anemia (RA), refractory anemia with ringed siderblasts (RARS), (refractory anemia with excess blasts (RAEB), and RAEB in transformation (RAEB-T); and myeloproliferative syndromes; brain, including glioma/glioblastoma, anaplastic oligodendroglioma, and adult anaplastic astrocytoma; neuroendocrine, including metastatic neuroendocrine tumors; head and neck, including , e.g., squamous cell carcinoma of the head and neck, and nasopharyngeal cancer; oral cavity; and pharynx; small intestine; bone; soft tissue sarcoma; and villous colon adenoma.
[00128] In some embodiments, compounds of the invention are suitable for the treatment of breast cancer, bladder cancer, colon cancer, glioma, glioblastoma, lung cancer, hepatocellular cancer, gastric cancer, melanoma, thyroid cancer, endometrial cancer, renal cancer, cervical cancer, pancreatic cancer, esophageal cancer, prostate cancer, brain cancer, or ovarian cancer.
[00129] In other embodiments, compounds of the invention are suitable for the treatment of inflammatory and cardiovascular disorders including, but not limited to, allergies/anaphylaxis, acute and chronic inflammation, rheumatoid arthritis; autoimmunity disorders, thrombosis, hypertension, cardiac hypertrophy, and heart failure.
[00130] Accordingly, in another aspect of the present invention, pharmaceutical compositions are provided, wherein these compositions comprise any of the compounds as described herein, and optionally comprise a pharmaceutically acceptable carrier, adjuvant or vehicle. In certain embodiments, these compositions optionally further comprise one or more additional therapeutic agents. [00131] It will also be appreciated that certain of the compounds of present invention can exist in free form for treatment, or where appropriate, as a pharmaceutically acceptable derivative thereof. According to the present invention, a pharmaceutically acceptable derivative includes, but is not limited to, pharmaceutically acceptable prodrugs, salts, esters, salts of such esters, or any other adduct or derivative which upon administration to a patient in need is capable of providing, directly or indirectly, a compound as otherwise described herein, or a metabolite or residue thereof.
[00132] As used herein, the term "pharmaceutically acceptable salt" refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. A "pharmaceutically acceptable salt" means any non-toxic salt or salt of an ester of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof. As used herein, the term "inhibitorily active metabolite or residue thereof means that a metabolite or residue thereof is also an inhibitor of VPS34 and/or PI3K.
[00133] Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge et al., describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference. Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclop entanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N+(Ci_4alkyl)4 salts. This invention also envisions the quaternization of any basic nitrogen-containing groups of the compounds disclosed herein. Water or oil-soluble or dispersable products may be obtained by such quaternization. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate.
[00134] As described above, the pharmaceutically acceptable compositions of the present invention additionally comprise a pharmaceutically acceptable carrier, adjuvant, or vehicle, which, as used herein, includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired. Remington's Pharmaceutical Sciences, Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa., 1980) discloses various carriers used in formulating pharmaceutically acceptable compositions and known techniques for the preparation thereof. Except insofar as any conventional carrier medium is incompatible with the compounds of the invention, such as by producing any undesirable biological effect or otherwise interacting in a deleterious manner with any other component(s) of the pharmaceutically acceptable composition, its use is contemplated to be within the scope of this invention. Some examples of materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, or potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, wool fat, sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil; safflower oil; sesame oil; olive oil; corn oil and soybean oil; glycols; such a propylene glycol or polyethylene glycol; esters such as ethyl oleate and ethyl laurate; agar; buffering agents such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethyl alcohol, and phosphate buffer solutions, as well as other non-toxic compatible lubricants such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, releasing agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the composition, according to the judgment of the formulator.
[00135] In yet another aspect, a method for treating a proliferative, inflammatory, or cardiovascular disorder is provided comprising administering an effective amount of a compound, or a pharmaceutical composition to a subject in need thereof. In certain embodiments of the present invention an "effective amount" of the compound or pharmaceutical composition is that amount effective for treating a proliferative, inflammatory, or cardiovascular disorder, or is that amount effective for treating cancer. In other embodiments, an "effective amount" of a compound is an amount which inhibits binding of PI3K and thereby blocks the resulting signaling cascades that lead to the abnormal activity of growth factors, receptor tyrosine kinases, protein serine/threonine kinases, G protein coupled receptors and phospholipid kinases and phosphatases.
[00136] The compounds and compositions, according to the method of the present invention, may be administered using any amount and any route of administration effective for treating the disease. The exact amount required will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the disorder, the particular agent, its mode of administration, and the like. The compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage. The expression "dosage unit form" as used herein refers to a physically discrete unit of agent appropriate for the patient to be treated. It will be understood, however, that the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific effective dose level for any particular patient or organism will depend upon a variety of factors including the disease being treated and the severity of the disease; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed, and like factors well known in the medical arts. The term "patient", as used herein, means an animal, preferably a mammal, and most preferably a human.
[00137] The pharmaceutically acceptable compositions of this invention can be administered to humans and other animals orally, rectally, parenterally, intracisternally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated. In certain embodiments, the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.
[00138] Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
[00139] Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S. P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables.
[00140] The injectable formulations can be sterilized, for example, by filtration through a bacterial- retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
[00141] In order to prolong the effect of a compound of the present invention, it is often desirable to slow the absorption of the compound from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then depends upon its rate of dissolution that, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered compound form is accomplished by dissolving or suspending the compound in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the compound in biodegradable polymers such as polylactide-polyglycolide. Depending upon the ratio of compound to polymer and the nature of the particular polymer employed, the rate of compound release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations are also prepared by entrapping the compound in liposomes or microemulsions that are compatible with body tissues.
[00142] Compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
[00143] Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar— agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may also comprise buffering agents.
[00144] Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.
[00145] The active compounds can also be in micro-encapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch. Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose. In the case of capsules, tablets and pills, the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes.
[00146] Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches. The active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required. Ophthalmic formulation, ear drops, and eye drops are also contemplated as being within the scope of this invention. Additionally, the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body. Such dosage forms can be made by dissolving or dispensing the compound in the proper medium. Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel.
[00147] While one or more of the inventive compounds may be used in an application of monotherapy to treat a disorder, disease or symptom, they also may be used in combination therapy, in which the use of an inventive compound or composition (therapeutic agent) is combined with the use of one or more other therapeutic agents for treating the same and/or other types of disorders, symptoms and diseases. Combination therapy includes administration of the therapeutic agents concurrently or sequentially. Alternatively, the therapeutic agents can be combined into one composition which is administered to the patient.
[00148] In one embodiment, the compounds of this invention are used in combination with other therapeutic agents, such as other inhibitors of VPS34 and/or PI3K. In some embodiments, a compound of the invention is administered in conjunction with a therapeutic agent selected from the group consisting of cytotoxic agents, radiotherapy, and immunotherapy. It is understood that other combinations may be undertaken while remaining within the scope of the invention.
[00149] Another aspect of the invention relates to inhibiting VPS34 and /or PI3K, activity in a biological sample or a patient, which method comprises administering to the patient, or contacting said biological sample with a compound of formula IA, IIA, IIIA, or IVA, or a composition comprising said compound. The term "biological sample", as used herein, generally includes in vivo, in vitro, and ex vivo materials, and also includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
[00150] Still another aspect of this invention is to provide a kit comprising separate containers in a single package, wherein the inventive pharmaceutical compounds, compositions and/or salts thereof are used in combination with pharmaceutically acceptable carriers to treat disorders, symptoms and diseases where VPS34 and/or PI3K kinase plays a role.
EXPERIMENTAL PROCEDURES
[00151] I-A. PREPARATION OF CERTAIN EXEMPLARY COMPOUNDS: Compounds (Shown in Table 1 below) were prepared using the general methods and specific examples described herein. [00152] EXAMPLES
[00153] Table 1 below depicts certain compounds represented by compounds of general formula IA, and subsets IIA, IIIA, IVA, or VA.
[00154] Table 1
Figure imgf000106_0001
1-24 1-19
The compounds of Table 1 above may also be identified by the following chemical names
Figure imgf000106_0002
Compound Name
2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-lH-imidazole-5-
1-19
carboxamide
[00157] Definitions
AA LCMS method using ammonium acetate
ACN acetonitrile
AcOH acetic acid
BOC tert-butoxycarbonyl
C Celsius
dba dibenzylideneacetone
DCE dichloroethane
DCM dichloromethane
DIEA diisopropylethylamine
DMAP 4-dimethylaminopyridine
DME 1,2-dimethoxyethane
DMF dimethylformamide
DMF-DMA dimethylformamide dimethylacetal
dppf 1 , 1 '-bis(diphenylphosphino)ferrocene
DMSO dimethylsulfoxide
EtOAc ethyl acetate
FA LCMS method using formic acid
h hours
HPLC high pressure liquid chromatography
HATU 0-(7-azabenzotriazol- 1 -yl)-NNN',N'-tetramethyluronium hexafluorophosphate
IC5o inhibitory concentration 50% KOH potassium hydroxide
LCMS liquid chromoatography mass spectrometry
m/z mass to charge
MeOH methanol
min minutes
MS mass spectrum
NBS N-bromosuccinimide
NCS N-chlorosuccinimide
PCC pyridinium chlorochromate
psi pounds per square inch
rt room temperature
SEM silylethoxymethyl
SiliaCat DPP-Pd diphenylphosphine palladium (II) heterogeneous silica-based catalyst
STAB sodium triacetoxyborohydride
TEA triethylamine
TFA trifluoroacetic acid
THF tetrahydrofuran
TBAF tetrabutylammoniumfluoride
TBTU 0-(benzotriazol- 1 -yl)-N,NN',N'-tetramethyluronium tetrafluoroborate
TMS trimethylsilyl
Xantphos 4,5-bis(diphenylphosphino)- 9,9-dimethylxanthene
[00158] Analytical LCMS Methods
[00159] LCMS spectra were recorded on a Hewlett-Packard HP 1 100 or Agilent 1 100 Series LC system connected to a Micromass mass spectromteter using reverse phase CI 8 columns. Various gradients and run times were selected in order to best characterize the compounds. Mobile phases were based on ACN/water gradients and contained either 0.1% formic acid (methods indicated FA) or 10 mM ammonium acetate (methods indicated AA). One example of a solvent gradient that was used was 100% mobile phase A (mobile phase A = 99% water + 1% ACN + 0.1% formic acid) to 100% mobile phase B (mobile phase B = 95% ACN + 5% water + 0.1% formic acid) at a flow rate of 1 mL/min for a 16.5 min run.
[00160] One of ordinary skill in the art will recognize that modifications of the gradient, column length, and flow rate are possible and that some conditions may be suitable for compound characterization than others, depending on the chemical species being analyzed.
[00161] Example 1: Synthesis of A'-[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)pyridin-2- yl]acetamid
Figure imgf000109_0001
[00162] Step 1: N-(4-bromopyridin-2-yl)acetamide
[00163] To a solution of 4-bromopyridin-2-amine (12.0 g, 69.4 mmol) in acetic anhydride (240 mL) was added DMAP (0.0847 g, 0.694 mmol). The reaction mixture was allowed to stir at 140 °C for 3 h and then allowed to cool to rt. Ice water was added and the pH of the mixture was adjusted to 8.5 by the addition of concentrated NH4OH. The solid which precipitated was filtered, washed with cold water and hexanes, and dried to give N-(4-bromopyridin-2-yl)acetamide (13.3 g) as a white solid.
[00164] Step 2: Af-[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)pyridin-2-yl]acetamide
[00165] To a mixture of N-(4-bromopyridin-2-yl)acetamide (17.2 g, 80 mmol, 1.0 equiv.), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi-l,3,2-dioxaborolane (26.4 g, 104 mmol), Pd(dppf)Cl2 (1 1.7 g, 16 mmol) and KOAc (23.6 g, 240 mmol) under an atmosphere of nitrogen was added anhydrous DMF (1500 mL). The mixture was allowed to stir at 80 °C for 3.5 h. The solvent was removed and the residue was diluted with EtOAc (1000 mL). Activated carbon (100 g) was added. The slurry was heated at reflux for 5 min and then filtered. The organic solution was concentrated and the residue was re-crystallized from EtOAc to give N-[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)pyridin-2-yl]acetamide (6.1 g, 29%) as a white solid. lH NMR (400 MHz, DMSO-i/6): δ 1.29 (s, 12H), 2.09 (s, 3H), 7.24 (dd, J = 6.0, 1.2 Hz, 1H), 8.30-8.33 (m, 2H), 10.47 (br s, 1H).
[00166] Compounds in the following table were prepared from the appropriate starting materials using the procedures described above:
Figure imgf000109_0002
[00167] Example 2: Synthesis of 2-[2-(acetylamino)pyridine-4-yl]-4-(2,4-dichlorophj
Figure imgf000110_0001
[00168] Step 1: methyl 2,4-dibromo-lH-imidazole-5-carboxylate
[00169] Methyl lH-imidazole-5-carboxylate (1.00 g, 7.93 mmol) was dissolved in ACN (50 mL) and NBS (3.53 g, 19.8 mmol) was added. The reaction mixture turned orange and was allowed to stir at rt for 5 h. The reaction mixture was then concentrated and the residue was dissolved in EtOAc. The organic solution was washed with Na2S203 and brine, dried over Na2S04 and concentrated. The residue was purified by column chromatography to give methyl 2,4-dibromo-lH-imidazole-5-carboxylate (0.798 g, 35%). LCMS (AA ): m/z = 285 (Μ+Η).
[00170] Step 2: methyl 2,4-dibromo-l-{[2-(trimethylsilyl)ethoxy]methyl}-lH-imidazole-5- carboxylate
[00171] To a suspension of NaH (0.220 g, 60% in mineral oil) in THF (5 mL) at 0 °C was added methyl 2,4-dibromo-lH-imidazole-5-carboxylate (1.50 g, 5.28 mmol) in THF (13 mL) slowly. The reaction mixture was allowed to warm to rt and to stir for 1.5 h. [2-(chloromethoxy)ethyl]- (trimethyl)silane (0.982 mL, 5.55 mmol) was added and the reaction mixture was allowed to stir at rt for 3.5 h. The reaction was quenched by the addition of aqueous sodium bicarbonate and the solution was extracted several times with DCM. The organic solutions were combined, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give two compounds, each with the mass of the desired product. These were assigned to be methyl 2,4-dibromo-l- {[2-(trimethylsilyl)- ethoxy]methyl}- lH-imidazole-5-carboxylate (first peak, less polar, 0.86 g, 39%) and the regioisomer, methyl 2,5-dibromo- 1 -((2-(trimethylsilyl)ethoxy)methyl)- lH-imidazole-4-carboxylate (second peak, more polar, 1.19 g, 54%). LCMS (FA, less polar product): m/z = 357 (Μ+Η). LCMS (FA, more polar product): m/z = 357 (Μ+Η).
[00172] Step 3: Methyl 2-(2-acetamidopyridin-4-yl)-4-bromo-l-{[2-(trimethylsilyl)- ethoxy]methyl}-lH-imidazole-5-carboxylate
[00173] Methyl 2,4-dibromo- 1 - { [2-(trimethylsilyl)ethoxy]methyl} - lH-imidazole-5-carboxylate
(0.860 g, 2.08 mmol), N-[4-(4,4,5,5-tetramethyl- l,3,2-dioxaborolan-2-yl)pyridin-2-yl]acetamide (0.544 g, 2.08 mmol) [l, -bis(diphenylphosphino)ferrocene]dichloropalladium(II) (1 : 1 complex with DCM, 0.170 g, 0.208 mmol) were dissolved in a mixture of 1 M aqueous sodium bicarbonate (4 mL) and DME (17 mL). The reaction mixtue was allowed to stir at reflux for 3 h, then was allowed to cool to rt and diluted with EtOAc. The mixture was washed with aqueous sodium carbonate and the organic solution was dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give methyl 2-(2-acetamidopyridin-4-yl)-4-bromo- 1 - { [2-(trimethylsilyl)-ethoxy]methyl} - lH-imidazole-5- carboxylate (0.42 g, 43%). LCMS (FA): m/z = 469.1 (Μ+Η).
[00174] Step 4: methyl 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l-{[2- (trimethylsilyl)ethoxy]methyl}-lH-imidazole-5-carboxylate
[00175] Methyl 2-[2-(acetylamino)pyridin-4-yl]-4-bromo- 1 - {[2-(trimethylsilyl)ethoxy]methyl} - 1H- imidazole-5-carboxylate (0.420 g, 0.895 mmol), 2,4-dichlorophenylboronic acid (0.213 g, 1.12 mmol), and [l,r-bis(diphenylphosphino)ferrocene]palladium(II)dichloride (0.0736 g, 0.0895 mmol) were dissolved in a mixture of 1 M aqueous sodium bicarbonate (1.8 mL) and DME (14 mL). The reaction mixtue was allowed to stir at reflux for 1.5 h, then was allowed to cool to rt and diluted with EtOAc. The mixture was washed with aqueous sodium carbonate and the organic solution was dried over Na2SO/t, filtered and concentrated. The residue was purified by column chromatography to give methyl 2-(2- acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)- 1 - { [2-(trimethylsilyl)ethoxy]methyl} - lH-imidazole-5- carboxylate (0.41 g, 86%). LCMS (AA): m/z = 537 (Μ+Η).
[00176] Step 5: 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l-{[2-(trimethylsilyl)- ethoxy] methyl}-lH-imidazole-5-carboxylic acid
[00177] Methyl 2-[2-(acetylamino)pyridin-4-yl]-4-(2,4-dichlorophenyl)-l- {[2-(trimethylsilyl)ethoxy]- methyl}-lH-imidazole-5-carboxylate (0.200 g, 0.373 mmol) was dissolved in THF (1.2 mL) and aqueous sodium hydroxide (1M, 1.12 mL, 1.12 mmol) was added. The reaction mixture was allowed to stir at rt for 17 h and then at 40 °C for 1 hour. Several drops of 50% NaOH were added and the reaction mixture was allowed to stir at 50 °C for 22 h. The reaction mixture was concentrated to remove the organic solvent. The aqueous solution was acidified to pH = 1 and then extracted with EtOAc. The organic solutions were combined and concentrated to give 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l- {[2-(trimethylsilyl)-ethoxy]methyl}-lH-imidazole-5-carboxylic acid, which was used in the next step without purification. LCMS (FA): m/z = 519 (Μ-Η).
[00178] Step 6: 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l-{[2-(trimethylsilyl)- ethoxy]methyl}-lH-imidazole-5-carboxamide
[00179] A solution of 2-[2-(acetylamino)pyridin-4-yl]-4-(2,4-dichlorophenyl)-l- {[2- (trimethylsilyl)ethoxy]methyl}-lH-imidazole-5-carboxylic acid (0.175 g, 0.336 mmol), TEA (0.356 mL, 2.56 mmol) and TBTU (0.431 g, 1.34 mmol) in DCM (4 mL) was allowed to stir at rt for 15 minutes. To the reaction mixture was added a solution of ammonia in 1,4-dioxane (0.5M, 3.57 mL, 1.78 mmol). The reaction mixture was allowed to stir at rt overnight and then was diluted with water and extracted with DCM. The organic solutions were combined, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l- {[2-(trimethylsilyl)-ethoxy]methyl}-lH-imidazole-5-carboxamide (0.066 g, 38%). LCMS (FA): m/z = 520 (Μ+Η).
[00180] Step 7: 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l-{[2-(trimethylsilyl)- ethoxy]methyl}-lH-imidazole-5-carboxamide
[00181] To a solution of 2-[2-(acetylamino)pyridin-4-yl]-4-(2,4-dichlorophenyl)-l- {[2- (trimethylsilyl)ethoxy]methyl}-lH-imidazole-5-carboxamide (0.066 g, 0.13 mmol) in DCM (4 mL) was added TFA (1.22 mL, 15.8 mmol). The reaction mixture as allowed to stir at rt for 4 h and was then concentrated. The residue was purified by HPLC to give 2-(2-acetamidopyridin-4-yl)-4-(2,4- dichlorophenyl)-l- {[2-(trimethylsilyl)ethoxy]methyl}-lH-imidazole-5-carboxamide (0.007 g, 14%). LCMS (FA): m/z = 390 (Μ+Η).
[00182] Example 3: Synthesis of 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2,l- a] [2,6]naphthyridine-3-carboxamide (1-24)
- I l l -
Figure imgf000113_0001
Figure imgf000113_0002
Figure imgf000113_0003
[00183] Step 1: methyl 2-(2-acetamido-5-bromopyridin-4-yl)-4-(2,4-dichlorophenyl)-lH- imidazole-5-carboxylate
[00184] To a solution of methyl 2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-l- {[2- (trimethylsilyl)ethoxy]methyl}-lH-imidazole-5-carboxylate (2.00 g, 3.73 mmol) in DMF (38 mL) was added NBS (1.33 g, 7.47 mmol). The reaction mixture was allowed to stir at 65 °C overnight. Additional NBS was added and the reaction mixture was allowed to stir at 65 °C for 1.5 h and then at rt for 4 d. Additional NBS was added and the reaction mixture was allowed to stir at 80 °C for 5 h and then allowed to cool to rt. The reaction mixture was diluted with saturated aqueous NaHCC>3 and extracted with EtOAc. The organic solutions were combined, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give methyl 2-(2-acetamido-5-bromopyridin-4-yl)-4- (2,4-dichlorophenyl)-lH-imidazole-5-carboxylate (1.58 g, 87%). LCMS (AA): m/z = 485 (Μ+Η).
[00185] Step 2: methyl 2-(2-acetamido-5-bromopyridin-4-yl)-4-(2,4-dichlorophenyl)-l-vinyl-lH- imidazole-5-carboxylate
[00186] To a solution of methyl 2-(2-acetamido-5-bromopyridin-4-yl)-4-(2,4-dichlorophenyl)- lH- imidazole-5-carboxylate (0.740 g, 1.53 mmol) and 1,2-dibromoethane (0.527 mL, 6.11 mmol) in DMF (6 mL) was added cesium carbonate (1.24 g, 3.82 mmol) and water (0.15 mL). The reaction mixture was allowed to stir at 70 °C for 5 h and then allowed to cool to rt and filtered through celite. The celite was washed with EtOAc and the filtrate was dried over Na2S04, filtered and concentrated. The residue was redissolved in DCM (6 mL) and DBU (0.571 mL, 3.82 mmol) was added. The reaction mixture was allowed to heat at reflux for 3 h and then allowed to cool to rt and concentrated. The residue was purified by column chromatography to give methyl 2-(2-acetamido-5-bromopyridin-4-yl)-4-(2,4-dichlorophenyl)-
1 -vinyl- lH-imidazole-5-carboxylate (0.263 g, 34%). LCMS (AA): m/z = 51 1 (Μ+Η).
[00187] Step 3: methyl 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2,l-a] [2,6]naphthyridine-3- carboxylate
[00188] A solution of methyl 2-(2-acetamido-5-bromopyridin-4-yl)-4-(2,4-dichlorophenyl)-l-vinyl- lH-imidazole-5-carboxylate (0.256 g, 0.502 mmol), palladium acetate (0.017 g, 0.075 mmol), tri-o- tolylphosphine (0.046 g, 0.150 mmol) and DIEA (0.315 mL, 1.81 mmol) in DMF (4.5 mL) was degassed with nitrogen and then sealed in a vial and subjected to microwave irradiation at 1 10 °C for 45 min and then at 125 °C for 90 min. Water was added to the reaction mixture and the aqueous mixture was extracted with EtOAc. The organic solutions were combined, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give methyl 9-acetamido-2-(2,4- dichlorophenyl)imidazo[2, l-a][2,6]naphthyridine-3-carboxylate (0.105 g, 49%). LCMS (FA): m/z = 429 (Μ+Η).
[00189] Step 4: 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2,l-a] [2,6]naphthyridine-3- carboxylic acid
[00190] To a solution of methyl 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2, l- a][2,6]naphthyridine-3-carboxylate (0.105 g, 0.245 mmol) in THF (2.3 mL) was added NaOH (1M in water, 0.788 mL, 0.788 mmol). The reaction mixture was allowed to heat at 50 °C for 24 h. Additional NaOH solution was added and the reaction mixture was allowed to heat at 60 °C for 5 h. The reaction mixture was allowed to cool to rt and then the pH was adjusted to 5-6 by the addition of 1M HCl. EtOAc was added and a yellow solid precipitated. The solid was filtered, rinsed with hexanes and dried under vacuum to give 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2, l-a][2,6]naphthyridine-3-carboxylic acid (0.058 g, 57%)), which was used in the next step without purification. LCMS (FA): m/z = 415 (M+H).
[00191] Step 5: 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2,l-a] [2,6]naphthyridine-3- carboxamide
[00192] To a solution of 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2,l-a][2,6]naphthyridine-3- carboxylic acid (0.058 g, 0.140 mmol) in DCM (1.7 mL) was added TEA (0.148 mL, 1.06 mmol) and TBTU (0.179 g, 0.559 mmol). The reaction mixture was allowed to stir at rt for 14 min and then ammonia (0.5 M in 1,4-dioxane, 1.5 mL, 0.74 mmoL) was added. The reaction mixture was allowed to stir at rt overnight and then diluted with water and extracted with DCM. The organic solutions were combined, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give 9-acetamido-2-(2,4-dichlorophenyl)imidazo[2, l-a][2,6]naphthyridine-3- carboxamide (0.004 g, 6%). LCMS (AA): m/z = 414 (M+H).
[00193] Example 4: Synthesis of Af-{4-[l-(2,4-dichlorophenyl)-2-(lH-pyrazol-5-yl)-lH-imidazol- 4-yl] -5-methylpyridin-2-yl} acetamide (1-16)
Figure imgf000115_0001
[00194] Step 1: 2,4-dibromo-l-(2,4-dichlorophenyl)-lH-imidazole
[00195] A mixture of 2,4-dibromo- 1H- imidazole (0.850 g, 3.8 mmol), l,3-dichloro-4-fluorobenzene (2.2 mL, 18.8 mmol) and potassium carbonate (2.60 g, 18.8 mmol) in DMF (3.2 mL) was sealed in a vial and subjected to microwave irradiation at 200 °C for 3h. The reaction mixture was diluted with water and extracted with EtOAc. The organic solutions were combined, washed with water, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give 2,4-dibromo- 1- (2,4-dichlorophenyl)-lH-imidazole (0.250 g, 18%). LCMS (FA): m/z = 371.2 (Μ+Η).
[00196] Step 2: 5-[4-bromo-l-(2,4-dichlorophenyl)-lH-imidazol-2-yl]-l-{[2-(trimethylsilyl)- ethoxy]methyl}-lH-pyrazole
[00197] A mixture of 2,4-dibromo- l-(2,4-dichlorophenyl)-lH-imidazole (0.079 g, 0.21 mmol), tetrakis(triphenylphosphine)palladium(0) (0.025 g, 0.021 mmol), cesium carbonate (0.208 g, 0.639 mmol) and l-((2-(trimethylsilyl)ethoxy)methyl)pyrazole-5-boronic acid (0.062 g, 0.256 mmol) in 1,4-dioxane (1.1 mL) and water (0.2 mL) was sealed in a vial and subjected to microwave irradiation at 100 °C for 20 min. The reaction mixture was diluted with water and extracted with EtOAc. The organic solutions were combined, washed with water, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give 5-[4-bromo- l-(2,4-dichlorophenyl)-lH-imidazol-2-yl]-l- {[2- (trimethylsilyl-)ethoxy]methyl}-lH-pyrazole (0.030 g, 30%). LCMS (FA): m/z = 489.4 (Μ+Η).
[00198] Step 3: A^-{4-[l-(2,4-dichlorophenyl)-2-(l-{[2-(trimethylsilyl)ethoxy]methyl}-lH- pyrazol-5-yl)-lH-imidazol-4-yl]-5-methylpyridin-2-yl}acetamide
[00199] A mixture of 5-[4-bromo-l-(2,4-dichlorophenyl)-lH-imidazol-2-yl]-l- {[2-(trimethylsilyl- )ethoxy]methyl}-lH-pyrazole (0.082 g, 0.17 mmol), tetrakis(triphenylphosphine)palladium(0) (0.019 g, 0.017 mmol), cesium carbonate (0.164 g, 0.504 mmol) and N-[5-methyl-4-(4,4,5,5-tetramethyl-l,3,2- dioxaborolan-2-yl)pyridin-2-yl]acetamide (0.063 g, 0.227 mmol) in 1,4-dioxane (2 mL) and water (0.5 mL) was sealed in a vial and subjected to microwave irradiation at 150 °C for 20 min. The reaction mixture was diluted with water and extracted with EtOAc. The organic solutions were combined, washed with water, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give N- {4-[l-(2,4-dichlorophenyl)-2-(l- {[2-(trimethylsilyl)ethoxy]methyl}-lH- pyrazol-5-yl)-lH-imidazol-4-yl]-5-methylpyridin-2-yl}acetamide (0.018 g, 19%). LCMS (FA): m/z = 556.8 (Μ+Η).
[00200] Step 4: A^-{4-[l-(2,4-dichlorophenyl)-2-(lH-pyrazol-5-yl)-lH-imidazol-4-yl]-5- methylpy ridin-2-yl} acetamide
[00201] A mixture of N- {4-[l-(2,4-dichlorophenyl)-2-(l- {[2-(trimethylsilyl)ethoxy]methyl}-lH- pyrazol-5-yl)-lH-imidazol-4-yl]-5-methylpyridin-2-yl} acetamide (0.018 g, 0.032 mmol) and TFA (0.5 mL) in DCM (5 mL) was allowed to stir at rt for 2 hr and then concentrated. The residue was purified by column chromatography to give N- {4-[l-(2,4-dichlorophenyl)-2-(lH-pyrazol-5-yl)-lH-imidazol-4-yl]-5- methylpyridin-2-yl} acetamide (0.009 g, 60%). LCMS (FA): m/z = 429.5 (Μ+Η).
[00202] Compounds in the following table were prepared from the appropriate starting materials using the procedures described above:
1-10 LCMS (FA): m/z = 413.4 (Μ+Η).
[00203] Example 5: Λ^-{4-[2-(2-ο1ι1θΓορ1ιεηγ1)-1-(ργΓίΰίη-2-γ1)-1^ίπιίΰ3ζο1-4-γ1]ργΓίΰίη-2- yl} acetamide (1-105)
Figure imgf000117_0001
[00204] Step 1: 2,4,5-tribromo-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-imidazole
[00205] To a suspension of NaH (60% in mineral oil) (0.68 g, 17.1 mmol) in THF (17 mL) at 0 °C was slowly added 2,4,5-tribromo-lH- imidazole (5.0 g, 16.4 mmol) in THF (42 mL). The reaction mixture was allowed to warm to rt and to stir for 2 h. The reaction was cooled to 0 °C and [2- (chloromethoxy)ethyl]-(trimethyl)silane (3.05 mL, 17.2 mmol) was added. The reaction mixture was allowed to stir at rt overnight. The reaction was quenched by the addition of aqueous sodium bicarbonate and the solution was extracted several times with DCM. The organic solutions were combined, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give 2,4,5- tribromo-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-imidazole (6.07 g, 85%). lB NMR (400 MHz, CDC13): δ 0.00 (s, 9H), 0.92-0.96 (m, 2H), 3.59-3.63 (m, 2H), 5.34 (s, 2H).
[00206] Step 2: 4,5-dibromo-2-(2-chlorophenyl)-l-((2-(trimethylsilyl)ethoxy)methyl)-lH- imidazole
[00207] A mixture of 2,4,5-tribromo-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-imidazole (0.456 g, 1.05 mmol), tetrakis(triphenylphosphine)palladium(0) (0.121 g, 0.105 mmol), cesium carbonate (1.02 g, 3.14 mmol) and 2-chlorophenylboronic acid (0.164 g, 1.05 mmol) in 1,4-dioxane (5.2 mL) and water (1.0 mL) was sealed in a vial and subjected to microwave irradiation at 100 °C for 20 min. The reaction mixture was diluted with water and extracted with EtOAc. The organic solutions were combined, washed with water, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give 4,5-dibromo-2-(2-chlorophenyl)-l-((2-(trimethylsilyl)ethoxy)methyl)-lH- imidazole (0.156 g, 32%). lH NMR (400 MHz, CDC13): δ 0.00 (s, 9H), 0.74-0.78 (m, 2H), 3.26-3.30 (m, 2H), 5.20 (s, 2H), 7.35-7.50 (m, 4H).
[00208] Step 3: 4-bromo-2-(2-chlorophenyl)-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-imidazole
[00209] To a solution of 4,5-dibromo-2-(2-chlorophenyl)-l-((2-(trimethylsilyl)ethoxy)methyl)-lH- imidazole (0.156 g, 0.354 mmol) in THF (10 mL) at at -78 °C was added «-butyllithium (2.5 M in hexane, 0.141 mL, 0.105 mmol). After allowing the reaction to stir at -78 °C for 2 min, water (0.40 mL) was added and the mixture was warmed to rt. The reaction mixture was treated with aqueous saturated ammonium chloride (10 mL) and extracted with EtOAc. The organic solutions were combined, washed with water, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give 4-bromo-2-(2-chlorophenyl)- 1 -((2-(trimethylsilyl)ethoxy)methyl)- lH-imidazole (0.090 g, 60%). ι¥ί NMR (400 MHz, CDC13): δ 0.00 (s, 9H), 0.75-0.79 (m, 2H), 3.26-3.31 (m, 2H), 5.11 (s, 2H), 7.16 (s, 1H), 7.36-7.48 (m, 4H).
[00210] Step 4: ^-(4-(2-(2-chlorophenyl)-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-imidazol-4- yl)pyridin-2-yl)acetamide
[00211] A mixture of 4-bromo-2-(2-chlorophenyl)-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-imidazole (0.055 g, 0.14 mmol), tetrakis(triphenylphosphine)palladium(0) (0.016 g, 0.014 mmol), sodium carbonate (0.060 g, 0.50 mmol) and N-[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)pyridin-2-yl]acetamide (0.045 g, 0.17 mmol) in 1,4-dioxane (1.4 mL) and water (0.37 mL) was sealed in a vial and subjected to microwave irradiation at 150 °C for 30 min. The reaction mixture was diluted with water and extracted with EtOAc. The organic solutions were combined, washed with water, dried over Na2S04, filtered and concentrated. The residue was purified by column chromatography to give N-(4-(2-(2-chlorophenyl)-l- ((2-(trimethylsilyl)ethoxy)methyl)-lH-imidazol-4-yl)pyridin-2-yl)acetamide (0.017 g, 27%). LCMS (FA): m/z = 443.4 (Μ+Η).
[00212] Step 5: Af-(4-(2-(2-chlorophenyl)-lH-imidazol-4-yl)pyridin-2-yl)acetamide
[00213] A mixture of N-(4-(2-(2-chlorophenyl)-l-((2-(trimethylsilyl)ethoxy)methyl)-lH-imidazol-4- yl)pyridin-2-yl)acetamide (0.090 g, 0.20 mmol) and TBAF (1.0M in THF, 2.0 mL, 2.0 mmol) in THF (6.2 mL) was allowed to stir at rt overnight. The reaction mixture was diluted with water and extracted with EtOAc. The organic solutions were combined, washed with water, dried over Na2SO/t, filtered and concentrated. The residue was purified by column chromatography to give N-(4-(2-(2-chlorophenyl)-lH- imidazol-4-yl)pyridin-2-yl)acetamide (0.040 g, 60%). LCMS (FA): m/z = 313.3 (Μ+Η).
[00214] Step 6: Λ^-{4-[2-(2-οΗ1θΓορΗεηγ1)-1-(ργΓίΰίη-2-γ1)-7^ίπιίΰ3ζο1-4-γ1]ργΓίΰίη-2- yljacetamide
[00215] A mixture of N-(4-(2-(2-chlorophenyl)-lH-imidazol-4-yl)pyridin-2-yl)acetamide (0.164 g, 0.524 mmol), potassium carbonate (0.362 g, 2.62 mmol) and 2-fluoropyridine (0.068 mL, 0.786 mmol) in DMF (3.0 mL) was was sealed in a vial and subjected to microwave irradiation at 150 °C for 5 h. The reaction mixture was diluted with water and extracted with EtOAc. The organic solutions were combined, washed with water, dried over Na2SO/t, filtered and concentrated. The residue was purified by column chromatography to give N- {4-[2-(2-chlorophenyl)-l-(pyridin-2-yl)-lH-imidazol-4-yl]pyridin-2- yljacetamide (0.095 g, 46%). LCMS (FA): m/z = 390.4 (Μ+Η).
[00216] BIOLOGICAL DATA: [00217] VPS34 Enzyme Assays
[00218] Cloning, expression, and purification of VPS34
[00219] VPS34 (accession number GB:BC033004) was cloned into ^DEST20-Thombin as N-terminal GST tagged fusion proteins using the Gateway system (Invitrogen, catalog# 1 1804-013). The sequences were verified before recombinant protein expression using the Baculovirus Expression System with Gateway® Technology.
[00220] For expression VPS34 was infected at 1MOI in SF9 cells and harvested 72 hours post infection.
[00221] For purification, VPS34 is purified by Glutathione Sepharose 4 Fast Flow (GE Healthcare
#17-5132-03) followed by HiTrap Q (GE Heaithcare #17- 1153-01).
[00222] VPS34 Assay Conditions
[00223] Human VPS34 enzyme assay method
[00224] lOOnL compounds in DMSO are added to wells of a 384 well mzcrotitre plate (Greiner 780076). At room temperature: 5ul VPS34 reaction buffer (Invitrogen Assay Buffer Q (diluted 1 in 5 with nanopure water) plus 2mM DTT and 2mM MnCl2) containing ATP (20uM, Promega) and 200uM PI-PS substrate (Invitrogen PV5122) is added followed immediately by 5ul VPS34 reaction buffer (as above) containing VPS34 (5nM, Millennium Protein Sciences Group) and the mixture is incubated with shaking at room temperature for 1 hour. Then 5ul VPS34 stop-detect mix (as per Invitrogen Adapta Assay kit (PV5009) instructions (contains kinase quench buffer, TR-FRET buffer, Adapta Eu anti-ADP antibody and Alexa Fluor 647 ADP tracer)) is added to quench the reaction. The plates are then incubated for 30 minutes at room temperature with shaking and then read on a BMG PheraStar Plus reader.
[00225] For the assay methods described above, test compound percent inhibition, at various concentrations, is calculated relative to control (DMSO and EDTA) treated samples. Compound concentration versus percent inhibition curves are fitted to generate IC5o values. One skilled in the art will appreciate that the values generated either as percentage inhibition at a single concentration or IC5o values are subject to experimental variation.
[00226] Vps34 Cell Assays
[00227] 1) FYVE domain redistribution assay
[00228] The FYVE domain redistribution assay monitors translocation of EGFP-2XFYVE from its initial location bound to (PtdIns(3)P) in early endosomes to the cytoplasm in response to test compounds. Recombinant U20S cells stable expressing the FYVE finger from the human homologue of the hepatocyte growth factor-regulated tyrosine kinase substrate Hrs, duplicated in tandem (GenBank Acc. NM 004712) and fused to the C-terminus of enhanced green fluorescent protein (EGFP). U20S cells are adherent epithelial cells derived from human osteosarcoma. Expression of EGFP-2X-FYVE is controlled by a standard CMV promoter and continuous expression is maintained by addition of geneticin to the culture medium. Localization of the fusion protein within the cells is imaged on the Evotec Technologies OPERA Confocal Imager and Integrated Spot Signal Per Cellular Signal is quantified using Acapella software. Using this information, IC50 values for inhibitors can be determined.
[00229] U20S EGFP-2XFYVE cells are propagated in Dulbecco's Modified Eagle Media High glucose(D-MEM) (Invitrogen cat. 1 1995) containing 10% Fetal Bovine Serum (HyClone cat.
SH30071.02) and 0.5mg/ml Geneticin (Invitrogen) and kept in a humidified chamber at 37°C with 5% C02. 8 x l03 cells are cultured in 100 μΐ of media per well in tissue culture-treated black- walled, clear bottom Optilux 96-well plates (BD Biosciences) for 16-24 hours.
[00230] Prior to addition of compounds, cell media is removed and replaced with 75 μΐ of fresh media. Test compounds in DMSO are diluted 1 : 100 in media. The diluted test compounds are added to the cells (25 μΐ per well) in 3-fold dilutions with a final concentration range of 0.0015 to 10 μΜ. The cells are incubated for 30 minutes in a humidified chamber at 37°C with 5% C02. Immediately following compound incubation, all liquid is removed from the wells and cells are fixed with 4% paraformaldehyde in PBS (75 μΐ per well) for 15 minutes at room temperature. The paraformaldehyde solution is removed from wells and washed once with PBS (100 μΐ per well). The PBS is removed and cells are incubated with DRAQ5 Nuclear Dye (Alexis/Biostatus) (85 μΐ per well). The plates are covered with Flash Plate plastic adhesive foil and imaged on the Evotec Technologies OPERA Confocal Imager Opera after at least a 30 minute incubation. Concentration curves are generated by calculating the Integrated Spot Intensity Per Cellular Signal decrease in test-compound treated samples relative to DMSO-treated controls and a 100% control inhibitor, and percentage inhibition values at a single concentration or growth inhibition (IC5o) values are determined from the curves. One skilled in the art will appreciate that the values generated either as percentage inhibition at a single concentration or IC5o values are subject to experimental variation.
[00231] PI3K Enzyme Assays
[00232] Cloning, expression, and purification of PI3Ks
[00233] The catalytic subunits of PI3Ks are cloned into either pDEST8(pl 10 alpha) or
pDEST10(pl lObeta, pi lOdelta, and p 1 1 Ogamma) as N-terminal His tagged fusion proteins using the Gateway system (Invitrogen, catalog# 1 1804-010 for pDEST8 and 1 1806-015 for pDESTIO). The sequences are verified before recombinant protein expression using the Baculovirus Expression System with Gateway® Technology. The accession numbers for the subunits are as follows:
pi 10 alpha (GB:U79143)
pl lObeta (GB:S67334)
pl lOdelta (GB: U86453) pi lOgamma (GB: X83368)
[00234] The regulatory subunits of PI3Ks are cloned into pDEST8 as un-tagged protein using the Gateway system (Catalog#l 1804-010). The sequences are verified before recombinant protein expression using the Baculovirus Expression System with Gateway® Technology. The accession numbers for the subunits are as following:
p85 alpha (GB: BC030815)
pl01(GB: AB028925)
VPS34 is cloned into pDEST20-Thombin as N-terminal GST tagged fusion proteins using the Gateway system (Invitrogen, catalog# 1 1804-013). The sequences are verified before recombinant protein expression using the Baculovirus Expression System with Gateway® Technology.
[00235] For expression of the pi 10 complexes, the p85 (MOI of 4) is co-infected with pi 10 alpha, beta, and delta respectively (1MOI) in SF9 cells and harvested at 60 hours post co-infection. PI 10 gamma was infected at 1 MOI and harvested at 60 hours post infection.
[00236] For purification, PI3Ks are purified by Ni-NTA Agarose (Qiagen #30250) followed by Mono
Q 10/100 GL (Ge Healthcare #17-5167-01). VPS34 is purified by Glutathione Sepharose 4 Fast Flow
(GE Healthcare #17-5132-03) followed by HiTrap Q (GE Healthcare #17- 1 153-01).
[00237] PI3K Assay Conditions
[00238] 1) Human PI3Ka enzyme assay method
[00239] 0.5uL compounds in DMSO are added to wells of a 384 well microtitre plate (Corning 3575). At room temperature: lOul PI3K reaction buffer (50 mM Hepes, 5mM DTT, 150mM NaCl, lOmM beta- glycerophosphate, lOmM MgCl2, 0.25mM sodium cholate and 0.001% CHAPS, pH 7.00) containing ATP (25uM, Promega) is added followed immediately by lOul PI3K reaction buffer containing di-C8 PI(4,5)P2 (3.5uM, CellSignals) and PBKalpha (0.4875nM, Millennium Protein Sciences Group) and the mixture is incubated with shaking at room temperature for 30 minutes. Then 5ul PI3K stop mix (50mM Hepes, 5mM DTT, 150 mM NaCl, 0.01% Tween-20, 15 mM EDTA and 25nM biotin-PI(3,4,5)P3 (Echelon) is added to quench the reaction followed immediately by addition of 5ul HTRF detection mix (50mM Hepes, 5mM DTT, 150 mM NaCl, 0.01% Tween-20, 40mM KF, ΙΟηΜ GST:GRP-1 PH domain (Millennium Protein Sciences Group), 15nM Streptavidin-XL (CisBio) and 0.375nM anti-GST Eu++ antibody (CisBio) at pH 7.00). The plates are then incubated for 1 hour at room temperature with shaking and then read on a BMG PheraStar Plus reader.
[00240] 2) Human PI3K beta, delta and gamma isoforms are tested using the procedure described for PI3K alpha above but with the following changes: PI3K beta (5.25nM), PI3K delta (0.75nM) and PI3K gamma (5nM). All isoforms supplied by Millennium Protein Science Group.
[00241] 3) VPS34 is assayed using Adapta™ Universal Kinase Assay Kit (Invitrogen). [00242] For the assay methods described above, test compound percent inhibition, at various concentrations, is calculated relative to control (DMSO and EDTA) treated samples. Compound concentration versus percent inhibition curves are fitted to generate IC5o values. One skilled in the art will appreciate that the values generated either as percentage inhibition at a single concentration or IC5o values are subject to experimental variation.
[00243] PI3K Cell Assays
[00244] 1) In-Cell Western Assay
[00245] The pSer473 AKT LI-COR In-Cell Western Assay is a quantitative immunofluorescent assay that measures phosphorylation of serine 473 AKT (pSer473 AKT) in WM266.4 and SKOV3 tumor cell lines grown in cell culture.
[00246] WM266.4 cells are propagated in Minimum Essential Media (MEM) (Invitrogen) containing L-glutamine, 10% Fetal Bovine Serum, 1 mM MEM Sodium Pyruvate, and 0.1 mM MEM Non-Essential Amino Acids and SKOV3 cells are propagated in McCoy's 5 A Media (modified) (Invitrogen) containing L-Glutamine and 10% Fetal Bovine Serum. Both cell lines are kept in a humidified chamber at 37°C with 5% C02. For the pSer473 AKT LI-COR In-Cell Western Assay, 1.5 x 104 WM266.4 and 1.5 x 104 SKOV3 cells are cultured in 100 μΐ of media per well in tissue culture-treated black- walled, clear bottom Optilux 96-well plates (BD Biosciences) for 16-20 hours. Prior to addition of compounds, cell media is removed and replaced with 75 μΐ of fresh media. Test compounds in DMSO are diluted 1 : 100 in media. The diluted test compounds are added to the cells (25 μΐ per well) in 3 -fold dilutions with a final concentration range of 0.0015 to 10 μΜ. The cells are incubated for 2 hours in a humidified chamber at 37°C with 5% CO2. Immediately following compound incubation, all liquid is removed from the wells and cells are fixed with 4% paraformaldehyde in PBS (150 μΐ per well) for 20 minutes at room temperature. The paraformaldehyde solution is removed from wells and the cells are permeabilized with 200 μΐ 0.1% Triton X- 100 in PBS per well for 10 min x 3 at room temperature. After removal of PBS + 0.1% Triton X-100, 150 μΐ Odyssey blocking buffer (LI-COR Biosciences) is added to each well and plates are incubated at room temperature for 1.5 h. Blocking buffer is removed from the wells and primary antibodies (Phospho-AKT (Ser473) (D9E) XP™ Rabbit mAb and AKT (pan) (40D4) Mouse mAb, Cell Signaling Technology) diluted in Odyssey blocking buffer are added (50 μΐ per well). Plates are incubated at 4°C overnight. The cells are washed for 20 min x 3 with PBS + 0.1% Tween-20 (200 μΐ per well). Secondary antibodies (IRDye 680 Goat anti-Rabbit IgG (H+L) and IRDye 800CW Goat anti- Mouse IgG (H+L), LI-COR Biosciences) are diluted in Odyssey blocking buffer and added to wells (50 μΐ per well) followed by a 1 h incubation at room temperature, protected from light. Cells are washed for 20 min x 3 with PBS + 0.1% Tween-20 (200 μΐ per well). Wash buffer is completely removed from wells after last wash, plates are protected from light until scanned and analyzed with the Odyssey Infrared Imaging System (LI-COR Biosciences). Both pS473 AKT and AKT are simultaneously visualized with the 680 nm fluorophore indicated by a red color and the 800 nm fluorophore indicated by a green color. Relative fluorescence units derived from the scans allow for quantitative analyses of both labeled proteins and the ratio of pS473 AKT to AKT is calculated. Concentration response curves are generated by plotting the average ratios of PI3K inhibitor-treated samples relative to DMSO-treated controls to determine percent change in expression of pS473 AKT, and percentage inhibition values at a single concentration or growth inhibition (IC5o) values are determined from those curves. One skilled in the art will appreciate that the values generated either as percentage inhibition at a single concentration or IC5o values are subject to experimental variation.
[00247] In some embodiments, compounds of the invention inhibit VPS34 at a 1.1 1 μΜ concentration with the percent inhibition as shown in the table below. In certain embodiments, compounds of the invention inhibit VPS34 with the IC50 values shown in the table below. In certain embodiments, compounds of the invention that inhibit VPS34 have an IC50 value A) less than 300 nM. In certain embodiments, compounds of the invention inhibit VPS34 have an IC5o value B) 300 nM - luM, C) greater than luM - 3uM, and D) greater than 3uM and less than lOuM.
Figure imgf000123_0001
IC50: A) less than 300 nM; B) 300 nM - luM, C) greater than luM - 3uM, and D) greater than 3uM-10uM
[00248] While we have described a number of embodiments of this invention, it is apparent that our basic examples may be altered to provide other embodiments, which utilize the compounds and methods of this invention. Therefore, it will be appreciated that the scope of this invention is to be defined by the appended claims rather than by the specific embodiments, which have been represented by way of example.

Claims

1. A compound of formula IA:
Figure imgf000124_0001
IA
or a pharmaceutically acceptable salt thereof, wherein:
-G1-G2-G3-G4- is -N=C-N-CR3=, =CR3-N-C=N-, =N-C=C-NR14-, or -NR14-C=C-N=;
each occurrence of R is independently hydrogen, cyclopropyl, or an optionally substituted group selected from Ci_6 aliphatic;
each occurrence of R3 is independently hydrogen, -CN, halogen, -Z-R5, or an optionally substituted group selected from Ci_6 aliphatic and 3- to 10-membered cycloaliphatic, wherein:
Z is selected from an optionally substituted Ci_3 alkylene chain, -0-,
-N(R3a)-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C02-, -C(0)NR3\ -N(R3a)C(0)-,
-N(R3a)C02-, -S(0)2NR3a-, -N(R3a)S(0)2-, -OC(0)N(R3a)-, -N(R3a)C(0)NR3a-,
-N(R3a)S(0)2N(R3a)-, or -OC(O)-;
R a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R5 is hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10- membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R1 is -CN, -C(0)N(R4)2, -C(0)OR4, -C(NR4)N(R4)2, -NHCOR4, -NHS02R4, -NHCON(R4)2, - NHCOOR4, -NHS02N(R4)2, -CH2OR4, -CH2N(R4)2, -CH2NHC(0)R4, -S02 N(R4)2, - C(0)NHC(=NH)N(R4)2, -NHS02OR4, or CY, wherein CY is an optionally substituted group selected from a 3- to -7-membered cycloaliphatic; a 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; a 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein:
each R is independently selected from hydrogen, -OH, or an optionally substituted group selected from d_6 aliphatic, 3- to 10-membered cycloaliphatic, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
R4 is -Z2-R6 wherein: Z2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-, -S(0)2-, -C(O)-, -CO2-, -C(0)NR4a-, -C(NH)-, or -S(0)2NR4a-,
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen, -NH2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or
two occurrences of R4, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0-1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R2 is hydrogen, halo, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10- membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein R2 is optionally substituted with 1-4 occurrences of R2a, wherein each occurrence of R2a is independently -R12a, -T2-R12d, -T2-R12a, or -V2-T2-R12d, and:
each occurrence of R12a is independently halogen, -CN, -N02, -R12c, -N(R12b)2,
-OR12b, -SR12c, -S(0)2R12c, -C(0)R12b, -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2,
-OC(0)N(R12b)2, -N(R12e)C(0)R12b, -N(R12e)S02R12c, -N(R12e)C(0)OR12b,
-N(R12e)C(0)N(R12b)2, or -N(R12e)S02N(R12b)2, or an optionally substituted Ci_6 aliphatic or Ci_6 haloaliphatic;
each occurrence of R12b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or two occurrences of R12b, taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
each occurrence of R12c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, Ci_6 haloaliphatic, 3- to 10-membered cycloaliphatic, 4- to 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each occurrence of R is independently hydrogen or an optionally substituted group selected from 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R12e is independently hydrogen or an optionally substituted
Ci_6 aliphatic group;
each occurrence of V2 is independently -N(R e)-, -0-, -S-, -S(O)-,
-S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R12e)-, -S(0)2N(R12e)-, -OC(0)N(R12e)-,
-N(R12e)C(0)-, -N(R12e)S02-, -N(R12e)C(0)0-, -N(R12e)C(0)N(R12e)-,
-N(R12e)S02N(R12e)-, -OC(O)-, or -C(0)N(R12e)-0-; and
T2 is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(R13)-, -0-, -S-, -S(O)-, -S(0)2-, -C(O)-, -C(0)0-, -C(0)N(R13)-,
-S(0)2N(R13)-, -OC(0)N(R13)-, -N(R13)C(0)-, -N(R13)S02-, -N(R13)C(0)0-, -N(R13)C(0)N(R13)-, - N(R13)S(0)2N(R13)-, -OC(O)-, or -C(0)N(R13)-0- or wherein T2 or a portion thereof optionally forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocyclyl ring, wherein R13 is hydrogen or an optionally substituted Ci_4 aliphatic group; and
HY is a
Figure imgf000126_0001
A , B , C ,
Figure imgf000126_0002
E , or F;
wherein
each occurrence of X4, X5, Χβ, Χη, and Xg is independently -CR10, -CR10 , or N, provided no more than two occurrences of X4, X5, Xe, Χη, and Xg is N;
each occurrence of Yi, Y2, Y3, Y4, Y5, Υβ, Ί, and Yg is -CR10;
each occurrence of Qi and Q2 is independently S, O or -NR9;
two adjacent occurrences of X4 and X5, Xe and X7, X7 and Xg, Yi and -NR9, Y3 and - NR9, or Y4 and Y5, may be taken together with the atoms to which they are bound, to form an unsubstituted fused heteroaryl or heterocyclyl group having 8 to 10 ring atoms and having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10 or R10' is independently -R10b, -V R10c, -T R10b, or -Vi-T R10b, wherein:
Vi is -NR11-, -NRu-C(0)-, -NRU-C(S)-, -NRU-C(NRU)-, -NRuC(0)0-, - NRuC(0)NRu-, -NRuC(0)S-, -NRuC(S)0-, -NRUC(S)NRU-, -NRUC(S)S-, - NRuC(NRu)0-, -NR11 CfNR1 ^NR11 -, -NRuS(0)2-, -N^^^NR11-, -C(O)-, -C02-, - C(0)NRu-, -C(0)NRuO-, -SO2-, or -S02NRu-;
each occurrence of R10a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
Ti is an optionally substituted Ci_6 alkylene chain wherein the alkylene chain optionally is interrupted by -N(RU)-, -0-, -S-, -S(O)-, -S(0) , -C(O)-, -C(0)0-, - C(0)N(Ru)-, -S(0)2N(Ru)-, -OC(0)N(Ru)-, -N(Ru)C(0)-, -N(Ru)S02-, - N(Rlla)C(0)0-, -N(R10a)C(O)N(R10a)-, -N(R10a)S(O)2N(R10a)-, -OC(O)-, or -C(0)N(Ru)- O- or wherein Ti forms part of an optionally substituted 3- to -7 membered cycloaliphatic or heterocyclyl ring;
each occurrence of R10b is independently hydrogen, halogen, -CN, -N02, -N(RU)2, -OR10a, -SR10a, -S(O)2R10a, -C(O)R10a, -C(O)OR10a, -C(0)N(Ru)2,
-S(0)2N(Ru)2, -OC(0)N(Ru)2, -N(Ru)C(O)R10a, -N(Ru)SO2R10a, -N(Ru)C(O)OR10a, - N(Ru)C(0)N(Ru)2, or -N(Ru)S02N(Ru)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10- membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R10c is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or
R10a and R1<¾ taken together with a nitrogen atom to which they are bound, form an optionally substituted 4- to -7-membered heterocyclyl ring having 0- 1 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur; each occurrence of R11 is independently hydrogen, -C(0)Rl la, -C02RUa , -C(0)N(Rl la)2,
-C(0)N(Rlla)-ORlla, -S02RUa, -S02N(Rlla)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of Rl la is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
each occurrence of R9 is independently hydrogen, -C(0)R9a, -C02R9a, -C(0)N(R9b)2, -S02R9a, - S02N(R9b)2, or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9a is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
wherein each occurrence of R9b is independently hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- to 10-membered cycloaliphatic, 4- to 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- to 10-membered aryl, or 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R% taken together with the nitrogen atom to which they are bound, form an optionally substituted group selected from 3- to 6-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or 5- to 10-membered heteroaryl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and provided that when HY is a non- fused group then HY is substituted with at least one occurrence of R10 or R10', wherein R10 or R10' is:
-N(Ru)C(O)R10a, -C(0)N(Ru)2, or -NRuC(O)OR10a; or
-Vi-T R10b, wherein Vt is -NR11-, Tt is a d -C3 alkylene chain, and R10b is an optionally substituted 6- to 10-membered aryl ring or a 5- to 10-membered heteroaryl ring having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or Vi is -NRuC(0)NRu- , Ti is a Ci -C3 alkylene chain, and R10b is -OR10a; or
-Vi-R10c, wherein Vi is -NR11-, and R10c is a 5- to 10-membered heteroaryl ring having 1 - 5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and
wherein a substituent on HY and R14, taken together with the atoms to which they are bound, form an optionally substituted 4-7-membered heterocyclyl ring having 0- 1 additional heteroatoms selected from nitrogen, oxygen, or sulfur;
provided:
when R3 is hydrogen, then R1 is not
Figure imgf000129_0001
, wherein R' is hydrogen or -C(0)2tBu; when R3 is hydrogen, then R1 is not -CH2OCH2CH2SiMe3;
) when R2 is hydrogen and Gi and G3 are nitrogen, then formula IA does not exist as the tautomeric form where Gi is substituted with hydrogen;
, and R1 is 2-chloro-6-fluorophenyl, then HY is not
Figure imgf000129_0002
where R is an optionally substituted phenyl ring; o) when HY is a substituted thiazolyl ring, then R1 is not a substituted pyrrolidinyl ring;
when R2 and R3 are both hydrogen, then HY is not
Figure imgf000129_0003
, wherein R is OH or an optionally s
Figure imgf000129_0004
HY is not substituted with
Figure imgf000130_0001
Figure imgf000130_0002
Figure imgf000131_0001
Figure imgf000131_0002
- 130-
Figure imgf000132_0001
The compound of claim 1 , wherein R1 is CY and CY is
G - X3 X3-G
or 11
wherein:
Xi, X2, and X3, are each independently N, O, S, NR4 , or CR7, provided that only one of
Xi, X2, or X3 may be O or S;
Y9 is nitrogen or carbon;
Gi4 is CR7', -N= or -NR4'-, wherein:
R4 is independently hydrogen, -Z2-R6, optionally substituted
Ci_6 aliphatic, or optionally substituted 3- 10-membered cycloaliphatic, wherein:
Z2 is selected from an optionally substituted Q_3 alkylene chain, -S(O)-, -S(0)2-, -C(O)-, -CO2-, -C(0)NR4a-, or -S(0)2NR4\
R4a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R6 is hydrogen or an optionally substituted group selected from Ci_6 aliphatic, 3- 10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6- 10-membered aryl, or 5- 10- membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each occurrence of R7 and R7 is independently hydrogen, -CN, halogen, -NH2, -Z3-R8, Ci_6 aliphatic, or 3-10-membered cycloaliphatic, wherein:
Z3 is selected from an optionally substituted Ci_3 alkylene chain, -0-, -N(R7a)-, - S-, -S(O)-, -S(0) , -C(O)-, -CO2-, -C(0)NR7a-, -N(R7a)C(0)-, -N(R7a)C02-, -S(0)2NR7a-, -N(R7a)S(0)2-, -OC(0)N(R7a)-, -N(R7a)C(0)NR7a-, -N(R7a)S(0)2N(R7a)-, or -OC(O)-;
R7a is hydrogen or an optionally substituted Ci_4 aliphatic, and
R8 is hydrogen or an optionally substituted group selected from d_6 aliphatic, 3-10-membered cycloaliphatic, 4- 10-membered heterocyclyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, 6-10-membered aryl, or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
Figure imgf000133_0001
3. The compound of claim 2, wherein CY is 1
4. The compound of claim 3, wherein Y9 is carbon, Xi is nitrogen, G14 is N(R4 ), and X2 and X3, are CH.
5. The compound of claim 3, wherein Y9 is carbon, Xi and X3 are nitrogen, G14 is N(R4 ), and X2 is CH.
6. The compound of claim 3, wherein Y9 is carbon, X! and GM are nitrogen, X3 is N(R4 ), and X2 is CH.
7. The compound of claim 3, wherein Y9 is carbon, Xi and X2 are nitrogen, G14 is N(R4 ), and X3 is CH.
8. The compound of claim 3, wherein Y9 is carbon, G14 is N(R4 ), X3 is nitrogen, and Xi and X2 CH.
9. The compound of claim 3, wherein Y9 is carbon, G14 is nitrogen, X3 is N(R4 ), and Xi and X2 are CH.
10. The compound of claim 3, wherein Y9 is carbon, X3 is nitrogen, X2 is N(R4 ), and Xi and Gi4 are CH.
1 1. The compound of claim 3, wherein Y9 is carbon, X2 is nitrogen, Gi4 is N(R4 ), and Xi and X3, are CH.
12. The compound of claim 3, wherein Y9 is carbon, X2 is N(R4 ), Gi4 is nitrogen, and Xi and X3, are CH.
13. The compound of claim 1, wherein R1 is Cy, and Cy is an optionally substituted 5- to 6- membered heteroaryl or heterocyclyl ring.
Figure imgf000134_0001
and Cy is optionally further substituted with one or more occurrences of R7 or R4'.
15. The compound of claim 1, wherein R1 is Cy, and Cy is an optionally substituted 6-membered aryl ring.
16. The compound of claim 1 , wherein R1 is -CON(R4)2, -C(0)OR4, -NHCOR4, or -CH2OR4.
17. The compound of any one of claims 1 to 16, wherein HY is selected from:
Figure imgf000135_0001
H J P
Figure imgf000135_0002
M Z
18. The compound of claim 17, wherein HY is selected from:
Figure imgf000135_0003
Figure imgf000135_0004
Figure imgf000135_0005
Figure imgf000136_0001
wherein each fused HY group is unsubstituted, and
each non- fused HY group is substituted with one or more occurrences of R10 or R10 , and at least one occurrence of R10 or R10' is -N(Ru)C(O)R10a, -N(Ru)C(O)OR10a or -C(0)N(Ru)2, and the dashed line represents a single bond or a double bond.
19. The compound of any one of claims 1 to 18, wherein R10a is Ci_6 aliphatic substituted with a 5-10- membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
20. The compound of claim 18, wherein HY is selected from:
Figure imgf000136_0002
wherein each fused HY group is unsubstituted, and
each non- fused HY group is substituted with one or more occurrences of R10 or R10 , and at least one occurrence of R10 or R10' is -N(Ru)C(O)R10a, -N(Ru)C(O)OR10a or -C(0)N(Ru)2, and the dashed line represents a single bond or a double bond.
Figure imgf000137_0001
21. The compound of claim 20, wherein HY is , and HY is substituted with one or more occurrences of R10 or R10 .
Figure imgf000137_0002
22. The compound of claim 21, wherein HY is , wherein R10 is hydrogen, methyl, chloro, bromo, fluoro, CN, CF3, OR10a, COR10a, and R10 is NHCOR10a or -NHC(O)OR10a.
23. The compound of claim 22, wherein R10 is hydrogen, methyl, or chloro.
24. The compound of claim 22, wherein R10' is methyl, and R10 is -NHCOR10a.
25. The compound of any one of claims 1 to 21, wherein R10 is -NHR11, wherein R11 is an optionally substituted 5- to 10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
26. The compound of any one of claims 1 to 25, wherein R2 is a 6- 10-membered aryl or 5- 10- membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; optionally substituted with 1 -3 occurrences of R2a.
27. The compound of claim 26, wherein R2 is a phenyl group; optionally substituted with 1 to 4 independent occurrences of halogen, d_3 alkyl, -CN, d_3 haloalkyl, -(CH2)pN(R12b)2, -OR12b, - NHC(0)R12b, -NHC(0)NHR12b, -NHS(0)2R12b, -S(0)2R12c, -S(0)2N(R12b)2, -C(0)OR12b, -C(0)N(R12b)2, or -C(0)R12b, and wherein p is 0 to 3.
28. The compound of claim 27, wherein R2 is a phenyl group; optionally substituted with 1 to 4 independent occurrences of halogen, Ci_3 alkyl, -CN, Ci_3 haloalkyl, -CH2N(CH3)2, -OCi_3 alkyl, -OCi_3 haloalkyl, -SCi_3 haloalkyl, -NHC(0)Ci_3 alkyl, -NHC(0)NHCi_3 alkyl, -NHS(0)2Ci_3 alkyl, or -C(0)H.
29. The compound of claim 28, wherein R2 is a phenyl group substituted with 1 or 2 occurrences of halogen.
30. The compound of any one of claims 1 to 25, wherein R2 is a 3-10-membered cycloaliphatic, 4- 10- membered heterocyclyl having 1 -5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
31. The compound of claim 30, wherein R2 is an optionally substituted N-linked 3-, 4-, 5-, 6-, or 7-membered heterocyclyl ring, optionally substituted with one or more occurrences of R2a.
32. The compound of claim 31, wherein R2 is optionally substituted with one or more Ci_3 alkyl groups, -OR12b, or -NR12b.
33. The compound of any one of claims 1 to 25, wherein R2 is a Ci_6 aliphatic and each occurrence of R2a is independently -C(0)OR12b, -C(0)N(R12b)2, -S(0)2N(R12b)2, -N(R12e)C(0)R12b, or - N(R12e)S02R12c.
34. The compound of claim 1 , wherein R1 is CY, -CON(R4)2, -NHCOR4, or -COOR4;
R2 is an optionally substituted 6- 10-membered aryl or 5-10-membered heteroaryl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and
HY is selected from
Figure imgf000138_0001
Figure imgf000139_0001
wherein each fused HY group is unsubstituted, and
each non-fused HY group is substituted with one or more occurrences of R10 or R10 , and at least one occurrence of R10 or R10' is -N(Ru)C(O)R10a or -C(0)N(Ru)2, and the dashed line represents a single bond or a double bond.
35. The compound of any one of claims 1 to 34 having the structure of formula IIA:
Figure imgf000139_0002
IIA
or a pharmaceutically acceptable salt thereof.
The compound of any one of claims 1 to 34 having the structure of formula IIIA:
Figure imgf000139_0003
IIIA
or a pharmaceutically acceptable salt thereof.
The compound of any one of claims 1 to 34 having the structure of formula IVA:
Figure imgf000139_0004
IVA
or a pharmaceutically acceptable salt thereof. The compound of any one of claims 1 to 34 having the structure of formula VA:
Figure imgf000140_0001
VA
or a pharmaceutically acceptable salt thereof.
39. The compound of claim 1, wherein the compound is selected from:
N- {4-[2-(2-chlorophenyl)- 1 -(pyridin-2-yl)- 1 H-imidazol-4-yl]pyridin-2-yl} acetamide;
N- {4-[l-(2,4-dichlorophenyl)-2-(lH-pyrazol-5-yl)- lH-imidazol-4-yl]pyridin-2-yl}acetamide;
N- {4-[l-(2,4-dichlorophenyl)-2-(lH-pyrazol-5-yl)-lH-imidazol-4-yl]-5-methylpyridin-2- yl} acetamide;
9-acetamido-2-(2,4-dichlorophenyl)imidazo[2, 1 -a] [2,6]naphthyridine-3-carboxamide; and
2-(2-acetamidopyridin-4-yl)-4-(2,4-dichlorophenyl)-lH-imidazole-5-carboxamide,
or a pharmaceutically acceptable salt thereof.
40. A pharmaceutical composition comprising a compound of any one of claims 1 to 39, and a pharmaceutically acceptable carrier.
41. The pharmaceutical composition of claim 40, further comprising another therapeutic agent.
42. A method of treating a proliferative disorder in a patient comprising administering to said patient a therapeutically effective amount of a compound of any one of claims 1 to 39.
43. The method of claim 42, wherein the proliferative disorder is breast cancer, bladder cancer, colon cancer, glioma, glioblastoma, lung cancer, hepatocellular cancer, gastric cancer, melanoma, thyroid cancer, endometrial cancer, renal cancer, cervical cancer, pancreatic cancer, esophageal cancer, prostate cancer, brain cancer, or ovarian cancer.
44. A method of treating an inflammatory or cardiovascular disorder in a patient comprising administering to said patient a therapeutically effective amount of a compound of any one of claims 1 to 39.
45. The method of claim 44, wherein the inflammatory or cardiovascular disorder is selected from allergies/anaphylaxis, acute and chronic inflammation, rheumatoid arthritis, autoimmunity disorders, thrombosis, hypertension, cardiac hypertrophy, and heart failure.
46. A method for inhibiting VPS34 or PI3K activity in a patient comprising administering a composition comprising a therapeutically effective amount of a compound of any one of claims 1 to 39.
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