WO2013094890A1 - Composition for clearing hangovers containing mineral water - Google Patents

Composition for clearing hangovers containing mineral water Download PDF

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Publication number
WO2013094890A1
WO2013094890A1 PCT/KR2012/010198 KR2012010198W WO2013094890A1 WO 2013094890 A1 WO2013094890 A1 WO 2013094890A1 KR 2012010198 W KR2012010198 W KR 2012010198W WO 2013094890 A1 WO2013094890 A1 WO 2013094890A1
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Prior art keywords
mineral water
alcohol
composition
hangover
present
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PCT/KR2012/010198
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French (fr)
Korean (ko)
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윤건호
김대진
김정득
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가톨릭대학교 산학협력단
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/02—Treatment of water, waste water, or sewage by heating
    • C02F1/04—Treatment of water, waste water, or sewage by heating by distillation or evaporation
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/3262—Foods, ingredients or supplements having a functional effect on health having an effect on blood cholesterol
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/332—Promoters of weight control and weight loss
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/334—Foods, ingredients or supplements having a functional effect on health treating the effects of consuming alcohol, narcotics or other addictive behavior, e.g. treating hangover or reducing blood alcohol levels
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1578—Calcium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1584—Cobalt
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1588—Copper
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/16—Potassium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/161—Magnesium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1612—Manganese
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1614—Sodium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1626—Selenium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1632—Strontium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1634—Titanium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1636—Vanadium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1642—Zinc

Definitions

  • the present invention relates to a hangover composition containing mineral water.
  • Alcohol acts on the central nervous system of the brain to improve mood and forget about anguish, and is used as a means of socializing and to relieve stress. In ancient times, alcohol was used as a basic excipient for all drugs. However, with the recent diversification of society and economic growth, the alcohol consumption of modern man is increasing, causing social problems such as hangover and alcoholism related to heavy drinking.
  • the hangover after drinking alcohol is a physical and mental phenomenon.
  • the most common symptoms are nausea, vomiting, dizziness, thirst, lethargy, headache and muscle pain.
  • Alcohol metabolism in the body is acetaldehyde by alcohol dehydrogenase (ADH) and then oxidized by acetaldehyde dehydrogenase (ALDH) to acetic acid and finally urine and CO 2 is excreted.
  • ADH alcohol dehydrogenase
  • ADH acetaldehyde dehydrogenase
  • ADH acetaldehyde dehydrogenase
  • Chronic alcohol intake induces damage to the small intestine mucosa, resulting in digestion and metabolism of nutrients such as vitamin B12, folic acid, thiamin, amino acids (leucine, phenylalanine, glycine, methionine, etc.), calcium (Ca) and magnesium (Mg). In addition to the negative effects, it increases the excretion of some nutrients in the urine, causing malnutrition.
  • autoxidation reaction can be inhibited to some extent by antioxidants.
  • Minerals such as selenium, copper, zinc, and manganese are known as representative antioxidant nutrients, and BHA (butylated hydroxyanisole, a synthetic phenol-based antioxidant, which is widely used in recent years)
  • Butylated hydroxy toluene (BHT) is widely used due to its excellent antioxidant power and economical efficiency.
  • BHT butylated hydroxy toluene
  • Korean Patent Laid-Open Publication No. 2011-0112925 ripening sulfur, licorice, cinnamon, earth, alder, tofu, Contents using natural herbal medicines such as Baekchul, Cheongung, Angelica, Soybean, Soybean, and Dermis are disclosed, and Korean Patent No. 1069662 discloses persimmon, dried persimmon, and garlic fermented and aged using fermentation enzymes. Although the contents are disclosed, there is no example developed for the purpose of relieving hangover of mineral water.
  • Another object of the present invention is to provide a health functional food for hangover relief containing the composition for resolving hangover containing the mineral water according to the present invention as an active ingredient.
  • the present invention provides a hangover relieving composition
  • a hangover relieving composition comprising the marine mineral water taken from the 1100m underground of the hot spring area of 92-1 Geumjin 3-ri, Okgye-myeon, Gangneung-si, Gangwon-do, South Korea as an active ingredient to provide.
  • the mineral water is a mineral component, potassium 200 ⁇ 230mg / L, sodium 7000 ⁇ 9500mg / L, calcium 1400 ⁇ 1700mg / L, magnesium 900 ⁇ 1100mg / L, zinc 3 ⁇ 9mg / L , Strontium 25 ⁇ 35mg / L, Selenium 200 ⁇ 500ug / L, Vanadium 65 ⁇ 75ug / L, Germanium 0.5 ⁇ 1.5ug / L, Manganese 10 ⁇ 40ug / L, Cobalt 1-3ug / L, Titanium 600 ⁇ 950ug / L , Copper 3 ⁇ 9ug / L, lithium 0.02 ⁇ 0.09mg / L, chlorine ion 16000 ⁇ 19500mg / L, fluorine ion 1.5 ⁇ 3.0mg / L, bromine ion 40 ⁇ 56mg / L, sulfate ion 3000 ⁇ 4
  • the mineral water is a desalted and evaporated concentrate
  • the concentrate may contain 0.5 to 50% (v / v) based on the total weight of the composition.
  • the mineral water may have the effect of reducing the concentration of alcohol in the blood, reducing the oxidative stress caused by alcohol.
  • the mineral water increases the expression of alcohol dehydrogenase hepatic alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), increase the expression of antioxidant enzyme GPx (Glutathione peroxidase) It may be through having a hangover relieving activity.
  • ADH alcohol dehydrogenase hepatic alcohol dehydrogenase
  • ADH aldehyde dehydrogenase
  • GPx Glutathione peroxidase
  • the mineral water may have an activity of reducing the total cholesterol content and triglyceride content in the body increased due to alcohol.
  • the present invention provides a health functional food for hangover relief containing the composition according to the present invention as an active ingredient.
  • Hangover composition containing marine mineral water taken from 1100m underground of the hot spring area of Geumjin 3-ri, Okgi-myeon, Gangneung-si, Gangneung-si, Gangwon-do, South Korea reduces the concentration of increased blood alcohol in the body due to alcohol consumption , It has the effect of reducing oxidative stress caused by alcohol, and it also has good recovery of liver function, so it can be used to prepare effective foods after alcohol consumption, and also for the treatment of diseases that can be caused by alcohol consumption. There is also an effect that can be used.
  • 1 is a graph measuring the blood ethanol concentration of the group treated with saline with drinking water, mineral water group, non-drinking group in alcohol-ingested rats.
  • Figure 2 is a graph showing the amount of ADH expression in the group treated with physiological saline with drinking water, mineral water group in rats ingesting alcohol.
  • 3 is a graph showing the amount of ALDH expression in the group treated with physiological saline with drinking water, mineral water group for the rats ingested alcohol.
  • Figure 4 is a graph showing the expression level of GPx, an antioxidant enzyme in the group treated with saline with drinking water, mineral water in alcohol-ingested rats.
  • Figure 5 is a graph showing the liver weight by treatment group in the alcohol chronic rat model.
  • Figure 6 is a graph showing the pancreas weight by treatment group in the chronic alcohol intake rat model.
  • Figure 7 is a graph showing the results of the measurement of AST, a type of liver function test in alcoholic rat model of chronic intake.
  • ALT is a type of liver function test in a model of chronic alcohol intake of alcohol.
  • Figure 9 is a graph showing the measurement results of ALP which is a type of liver function test in a model of chronic alcohol intake of alcohol.
  • 10 is a graph showing the total cholesterol results in the chronic alcohol intake rat model.
  • FIG. 11 is a graph showing triglyceride levels, which is a triglyceride measurement method in a rat model of chronic alcohol intake.
  • FIG. 12 shows the results of microscopic observation of liver tissue tissue binding through hematoxylene-eosin staining after separating liver tissue from each rat group.
  • the present invention is characterized in that it provides a new hangover-relieving composition comprising mineral water as an active ingredient which reduces blood alcohol concentration, is excellent in reducing oxidative stress caused by alcohol, and is effective in improving liver function.
  • Mineral water contained as an active ingredient in the hangover composition according to the invention is characterized in that it is marine mineral water taken from the 1100m underground of the hot spring area, 92-1, Geumjin 3-ri, Okgye-myeon, Gangneung-si, Gangwon-do, South Korea.
  • seawater In general, seawater is called deep seawater, which is distinguished from surface water, and deep sea water refers to seawater 200 meters or less from the surface of seawater. It contains many inorganic nutrients such as nitrogen, phosphorus, and silicic acid needed for plant growth because photosynthesis does not occur. It is known that it is not polluted by air or chemicals and contaminated with solar bacteria or general bacteria, and has a small number of benign bacteria, so that the physical cleanliness is excellent.
  • the present inventors are developing a composition that can resolve hangover using marine mineral water.
  • the alcohol concentration By reducing the alcohol concentration, the effect of reducing the oxidative stress by alcohol, and the effect of improving the liver function was confirmed, it was confirmed that the mineral water is very suitable for use as a hangover composition.
  • the blood ethanol concentration was measured from a mouse ingesting the mineral water of the present invention and a mouse receiving the saline instead of the mineral water in the mouse model of acute alcoholism, Mice fed with mineral water showed a significant decrease in blood alcohol concentrations compared to control mice (see FIG. 1).
  • alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activity known as alcohol degrading enzymes to confirm whether the mineral water of the present invention can effectively resolve hangover alcohol
  • ADH alcohol dehydrogenase
  • ALDH aldehyde dehydrogenase
  • alcohol degrading enzymes to confirm whether the mineral water of the present invention can effectively resolve hangover alcohol
  • the present inventors can see that the mineral water of the present invention can increase the expression of alcohol degrading enzymes, and has an antioxidant activity that inhibits the oxidative action of the body by alcohol, so that the present invention can be used for hangover relief. there was.
  • the present inventors measured the expression levels of AST, ALT, and ALP, which are used as indicators of liver function tests, to determine whether the mineral water of the present invention can improve liver toxicity by alcohol. It was found that mineral water has the activity of inhibiting the three enzymes that increase the expression in the liver due to alcohol, and the degree of inhibition of expression of AST, ALT and ALP was proportional to the concentration of treated mineral water (Fig. 7 to 9).
  • the ALT is an alanine aminotransferase and an enzyme in hepatocytes, also called serum glutamic pyruvate aminotransferase (SGPT) or alanine aminotransferase (ALAT).
  • SGPT serum glutamic pyruvate aminotransferase
  • ALAT alanine aminotransferase
  • AST is also called aspartate aminotransferase or aspartate aminotransferase (ASAT) and is similar to ALT in that it is another enzyme associated with cells of liver tissue. This enzyme is also known to increase expression in acute liver injury.
  • ALP alkaline phosphatase
  • ALP is an enzyme inside the cells that surround the bile ducts, and plasma concentrations of ALP are known to rise with obstruction of the bile ducts, stopping bile secretion inside the liver, or diseases caused by invasive bacteria. have.
  • mineral water according to the present invention has an effect of reducing the concentration of total cholesterol and triglycerides in the body. As a result, it was confirmed that the total cholesterol and triglycerides, which are triglycerides, were effectively inhibited by the mineral water according to the present invention (see FIGS. 10 and 11).
  • the mineral water of the present invention has an effect of restoring liver damage caused by alcohol through liver tissue examination of an alcoholic mouse animal model (see FIG. 12).
  • the present inventors can effectively reduce the blood alcohol concentration, which is increased by the mineral water provided by the present invention, by promoting the expression of alcohol degrading enzymes, and also inhibits the oxidative stress caused by alcohol. It is effective to reduce the amount of total cholesterol and triglycerides accumulated in liver tissue, and to improve the function of the liver, and as a result, the mineral water of the present invention is ultimately used to relieve hangover. You can see that it can be used.
  • the present invention can provide a hangover relief composition comprising marine mineral water taken at 1100m underground from the hot spring area of 92-1, Geumjin 3-ri, Okgye-myeon, Gangneung-si, Gangwon-do, Korea.
  • the mineral water contained in the composition of the present invention as an active ingredient is a mineral component, potassium 200 ⁇ 230mg / L, sodium 7000 ⁇ 9500mg / L, calcium 1400 ⁇ 1700mg / L, magnesium 900 ⁇ 1100mg / L, zinc 3 ⁇ 9mg / L, Strontium 25 ⁇ 35mg / L, Selenium 200 ⁇ 500ug / L, Vanadium 65 ⁇ 75ug / L, Germanium 0.5 ⁇ 1.5ug / L, Manganese 10-40ug / L, Cobalt 1-3ug / L, Titanium 600 ⁇ 950ug / L, copper 3 ⁇ 9ug / L, lithium 0.02 ⁇ 0.09mg / L, chlorine ion 16000 ⁇ 19500mg / L, fluorine ion 1.5 ⁇ 3.0mg / L, bromine ion 40 ⁇ 56mg / L, sulfate ion 3000
  • This component analysis is a result of the mineral component analysis of the mineral water according to the present invention commissioned by the Korea Institute of Science and Technology (KIST), the Korea Basic Science Institute (KBSI) and the Korea Institute of Geoscience and Mineral Resources (KIGAM). Mineral waters are new to date.
  • KIST Korea Institute of Science and Technology
  • KBSI Korea Basic Science Institute
  • KIGAM Korea Institute of Geoscience and Mineral Resources
  • the mineral water may use the marine mineral water itself taken from the 1100m underground of the hot spring area, 92-1, Geumjin 3-ri, Okgye-myeon, Gangneung-si, Gangneung-si, Gangwon-do, South Korea, the mineral water is the total weight of the composition It can be used in an amount of 50% (v / v) to 100% (v / v) as a standard, or can be prepared and used as a concentrate, and when used as a concentrate, 0.5 to 50% based on the total weight of the composition It can be contained and used in the quantity of (v / v).
  • the concentrate may be a solid obtained by evaporating, concentrating and drying the mineral water according to the present invention, or may be a concentrate obtained by evaporating the mineral water by natural drying or by artificially desalting and drying (or evaporating) the mineral water.
  • the concentrate of the mineral water according to the present invention may be prepared by the following method.
  • the filtration step may be subjected to a plurality of filtration steps, in the present invention was carried out a four-step filtration process. That is, the first stage filtration was removed by filtering the suspended matter more than 10um, and then removed by filtering the suspended matter more than 5um again as a second stage filtration, and then removed by filtering the suspended matter above 1um as a three-step filtration.
  • the suspended solids filtered by 0.2um or more by a four-step filtration process.
  • the desalination is not limited thereto, but may be used an electrodialysis machine.
  • desalination powder is prepared through a drying step using natural drying or an artificial dryer.
  • a vacuum freeze dryer was used for the drying.
  • the desalted powder can be ground and packed.
  • the preparation of the concentrate of the mineral water according to the present invention may be carried out first through the drying process after collecting the raw water, and then performing the filtration step.
  • composition of the present invention may be used as a pharmaceutical composition for relieving hangover, such as lowering blood alcohol concentration, and when used in concentrated mineral water according to the present invention, it may include 0.5 to 50% by weight relative to the total weight of the composition. Can be.
  • composition for resolving hangover according to the present invention is safer than conventional chemical preparations using natural deep sea water as a raw material, and there are no toxic and side effects for drugs, so it can be used with confidence even during long-term use.
  • the composition of the present invention can be added to a food for the purpose of preventing or treating a disease caused by alcohol, a composition for food or a health functional food for hangover relief for the prevention and improvement of disease symptoms caused by alcohol It can be used as a composition.
  • Food which is effective in preventing and ameliorating disease symptoms caused by alcohol, for example, it can be easily utilized as a main ingredient, side ingredients, food additives, functional food or beverage of food.
  • the "food” means a natural product or processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through a certain processing step, and a general meaning As used herein, it refers to food, food additives, functional foods and beverages.
  • Foods to which the composition according to the present invention can be added include, for example, various foods, beverages, gums, teas, vitamin complexes, functional foods, and the like.
  • food includes special nutritional products (e.g., formulated milk, young, infant food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g. ramen, noodles, etc.), bread, health supplements, seasonings.
  • Foods e.g. soy sauce, miso, red pepper paste, mixed soy sauce
  • sauces confectionery (e.g. snacks), candy, chocolates, gums, ice creams, dairy products (e.g.
  • fermented milk, cheese, etc. other processed foods
  • kimchi, Pickled foods various kimchi, pickles, etc.
  • beverages e.g., fruit drinks, vegetable drinks, soy milk, fermented beverages, etc.
  • natural seasonings e.g. ramen soup, etc.
  • the food, beverage or food additives may be prepared by a conventional manufacturing method.
  • the term "functional food” refers to the control of biological defense rhythms and disease prevention of food groups or food compositions that have added value to the food by using physical, biochemical, or biotechnological techniques to act and express the function of the food for a specific purpose. It means a food that is designed and processed to fully express the body's regulatory function regarding recovery and the like, and specifically, it may be a health functional food.
  • the functional food may include food acceptable food additives, and may further include appropriate carriers, excipients and diluents commonly used in the manufacture of functional foods.
  • the "beverage” refers to a generic term for drinking to quench thirst or to enjoy a taste and includes a functional drink.
  • the beverage is not particularly limited in addition to the composition for eliminating hangover according to the present invention as an essential ingredient in the ratio indicated, and may contain various flavors or natural carbohydrates, etc. as an additional ingredient like a normal beverage. Can be.
  • foods containing the composition of the present invention include various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavoring agents, colorants and fillers (such as cheese and chocolate), and pectic acid. And salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like, which components may be independently or in combination Can be used.
  • the amount of the composition according to the present invention may comprise from 0.001% to 90% by weight of the total food weight, preferably from 0.1% to 40% by weight.
  • the amount of the composition according to the present invention may comprise from 0.001% to 90% by weight of the total food weight, preferably from 0.1% to 40% by weight.
  • it may be included in a ratio of 0.001g to 2g, preferably 0.01g to 0.1g based on 100ml, in the case of long-term intake for health and hygiene purposes or health control purposes It may be less than the range, the active ingredient is not limited to the above range because it may be used in an amount above the range because there is no problem in terms of safety.
  • the present invention can provide a health functional food for relieving hangover containing mineral water or mineral water concentrate according to the present invention as an active ingredient, but the form of the food is not limited thereto, but the powder, granule, tablet, capsule Or in the form of a drink.
  • the present inventors collected the hot spring water taken from the 110m underground bedrock of the Geumjin-ri beach coastal area of Okgye-myeon, Gangneung-si, Gangneung-si, Gangwon-do, to collect the mineral water that is effective in resolving hangovers. After removing the material, it was desalted using an electrodialysis machine (voltage: 5.5-7.5 V, electrode solution: 3% Na 2 SO 4 , CJTTS-2-10 ED, Creative Techno). After the desalination process was filtered and dried using a vacuum freeze dryer (temperature: -40 ⁇ 30 °C, vacuum degree: 5mTorr), the mineral water concentrate of the present invention prepared by this process in the following experiment Used.
  • an animal model ingesting alcohol was prepared as follows. 400 grams of healthy male Sprague Dawley rats, 9 weeks old, were bred for 2 weeks or more in a pathogen-free environment and were fed sterile standard rat feed (Ralston Purina, St. Louis, MO). Under the approval of the Institutional Animal Care and Use Committee. Thus, acute alcoholism mice and chronic alcoholism mice were made for the bred mice. First, the acute alcoholism mice were fasted for 18 hours before the experiment, and the following experiment was made by making three animal test groups as shown in Table 1 below. Were performed.
  • Table 1 Acute Alcoholism Mouse Model Group 1 (control) Normal mouse group that treated nothing with non-drinking group 2 Ingestion of 1 ml of saline solution before alcohol consumption 3 After ingesting 1ml mineral water of the present invention, the group ingested alcohol
  • mice were ingested with 20% alcohol in an amount of 2.5 ml per mouse.
  • the blood alcohol concentration was shown to show the highest point at 3 hours after the intake of alcohol, ingested the saline prior to alcohol intake and the mineral water of the present invention
  • the mineral water intake group significantly decreased blood alcohol concentration compared to the physiological saline intake group.
  • Example ⁇ 3-1> In order to determine whether the mineral water of the present invention has hangover-relieving activity, after 7 hours after ethanol ingestion in the experimental group of Example ⁇ 3-1>, each mouse was lethal and separated from liver tissue when the final blood collection was completed. Next, RNA was extracted from the tissue, and RT-using primers specific for the genes to analyze the expression levels of hepatic alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) expressed in liver tissue. PCR was performed and the base sequences of the primers used are as described below.
  • ADH hepatic alcohol dehydrogenase
  • ADH aldehyde dehydrogenase
  • ADH forward primer 5'-ctgtaaagcagcaggagcag-3
  • ADH reverse primer 5'-tcctacgacgacgcttacac -3 '
  • the present inventors have conducted antioxidant enzymes targeting RNA of liver tissues obtained in Example ⁇ 3-2> to check whether the mineral water of the present invention inhibits the oxidative stress known to occur when alcohol is ingested.
  • the base sequence of the primer of GPx used at this time is as follows.
  • mice were prepared to prepare chronic alcoholism mouse models.
  • Lieber-Decarli ethanol diet one of the methods to induce chronic fatty liver, to prepare chronic alcoholic mouse model.
  • Lieber-Decarli ethanol diet was 35% fat, 11% carbohydrate, 18% protein, 36 A diet containing% ethanol and 35% fat, 47% carbohydrate, and 18% protein was used as a Lieber-Decarli control diet.
  • the intake schedule for the four groups was first to 11 to 13 weeks of age, except for the control group (A) B, C, D group ethanol (3g / kg) intake, and 14 to 17 weeks
  • the diet shown in Table 2 was provided, and each mouse was sacrificed at 18 weeks, and then experiments were performed.
  • liver and pancreas weights were measured from each of the sacrificed mice. As a result, the liver and pancreas weights were decreased in the mineral water treated groups (C and D) compared to the ethanol-only group (B). , The weight loss was proportional to the concentration of mineral water ingested (see FIGS. 5 and 6).
  • AST, ALT and ALP indicators which are a type of liver function test, and the animals used in Example ⁇ 4-1>.
  • the model was performed on the subject.
  • AST and ALT are described in Reitman et al., Reitman, S., et al., A colormetric method for the determination of serum giutamic oxaloactic and glitamic pyruvic transaminase, Am. J. Clin. Pathol., 28, pp. 56-63, 1957) was used to analyze the kit (Asan Pharmaceutical), ALT (containing 1780mg of DL-alanine and 29.2mg of a-ketoglutamic acid per 100ml) and AST (2,660mg of L-aspartic acid per 100ml).
  • a-ketoglutamic acid containing 29.2 mg were preincubated with 1.0 ml of substrate solution at 37 ° C. for 5 minutes, and then 0.2 ml of each prepared serum was reacted at 37 ° C. for 30 minutes for ALT and 60 minutes for AST. After adding 1.0 ml of a coloring solution (containing 2,4-dinitrophenylhydrazine, 19.8 mg / 100 ml), and adding 1.0 ml of 0.4 N-NaOH solution, mixed and left at room temperature for 10 minutes and absorbance at wavelength 505 nm (Shimadzu UV-1201, Japan). ) was measured.
  • a coloring solution containing 2,4-dinitrophenylhydrazine, 19.8 mg / 100 ml
  • Blood was collected from the animal model used in Example ⁇ 4-1>, and total cholesterol and triglyceride levels were measured.
  • Total cholesterol was measured using a cholesterol assay kit (Daiichi Sankyo Company, Tokyo, Japan) and triglycerides were analyzed using an ADVIA 1650 (Bayer Co. Ltd., USA) automated analyzer using a triglyceride assay kit (Wako Pure chemical Industries, Osaka, Japan). And the results are shown in FIGS. 10 and 11.
  • the serum total cholesterol level was 60 ⁇ 10.8mg / dL in the ethanol group, and the mineral water treatment group was decreased compared to the ethanol treatment group, and the decrease was proportional to the treatment concentration of the mineral water.
  • Triglycerides, triglycerides also showed a decrease in triglyceride levels in the groups ingested 10% and 30% mineral water than in the ethanol group (similar to the results of total cholesterol) (see FIG. 11).
  • liver resection was performed on the mouse model used in Example ⁇ 4-1>, and the liver was treated with formaldehyde solution buffered with 10% phosphoric acid. After fixing the tissue, demineralized in 10% EDTA for 8 hours and embedded in paraffin. Tissue sections (7 um) were prepared and stained with hematoxylin and eosin and observed under a microscope.

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Abstract

The present invention relates to a composition for clearing hangovers containing mineral water as an active ingredient, and, more specifically, the present invention relates to a composition for clearing hangovers which comprises, as mineral constituents: between 200 and 230 mg/l of potassium, between 7,000 and 9,500 mg/l of sodium, between 1,400 and 1,700 mg/l of calcium, between 900 and 1,100 mg/l of magnesium, between 3 and 9 mg/l of zinc, between 25 and 35 mg/l of strontium, between 200 and 500 ug/l of selenium, between 65 and 75 ug/l of vanadium, between 0.5 and 1.5 ug/l of germanium, between 10 and 40 ug/l of manganese, between 1 and 3 ug/l of cobalt, between 600 and 950 ug/l of titanium, between 3 and 9 ug/l of copper, between 0.02 and 0.09 mg/l of lithium, between 16,000 and 19,500 mg/l of chloride ions, between 1.5 and 3.0 mg/l of fluoride ions, between 40 and 56 mg/l of bromide ions, between 3,000 and 4,500 mg/l of sulphate ions, between 0.8 and 1.2 mg/l of boron and between 0.5 and 15 mg/l of silicon dioxide.

Description

광천수를 함유한 숙취 해소용 조성물Hangover Relief Composition Containing Mineral Water
본 발명은 광천수를 함유한 숙취 해소용 조성물에 관한 것이다. The present invention relates to a hangover composition containing mineral water.
알코올은 뇌의 중추신경에 작용하여 기분을 좋게 하고 괴로움을 잊을 수 있으며, 사교의 수단 및 스트레스 해소를 위한 식품으로 활용되고 있어 고대에는 알코올이 모든 약물의 기본 부형제로 이용되었다. 하지만 최근 사회의 다양화 및 경제성장과 더불어 현대인의 알코올 섭취량이 증가하고 있어 과음과 관련된 숙취나 알코올 중독과 같은 사회문제가 발생하고 있다. Alcohol acts on the central nervous system of the brain to improve mood and forget about anguish, and is used as a means of socializing and to relieve stress. In ancient times, alcohol was used as a basic excipient for all drugs. However, with the recent diversification of society and economic growth, the alcohol consumption of modern man is increasing, causing social problems such as hangover and alcoholism related to heavy drinking.
음주 후 나타나는 숙취는 신체적, 정신적 현상으로 그 대표적인 증상으로는 메스꺼움, 구토, 현기증, 갈증, 무기력함, 두통, 근육통 등이 있다. 체내의 알코올 대사는 알코올탈수소효소(alcohol dehydrogenase, ADH)에 의해 아세트알데히드(acetaldehyde)가 되고 다시 아세트알데히드탈수소효소(acetaldehyde dehydrogenase, ALDH)에 의해 산화되어 아세트산(acetic acid)이 되고 최종적으로는 뇨와 CO2 등으로 배설된다. The hangover after drinking alcohol is a physical and mental phenomenon. The most common symptoms are nausea, vomiting, dizziness, thirst, lethargy, headache and muscle pain. Alcohol metabolism in the body is acetaldehyde by alcohol dehydrogenase (ADH) and then oxidized by acetaldehyde dehydrogenase (ALDH) to acetic acid and finally urine and CO 2 is excreted.
알코올이 산화되어 만들어진 일차 대사산물인 아세트알데히드(acetaldehyde)는 숙취의 주요한 인자이며, 알코올 섭취 시 체내의 독성작용을 통해 알코올 그 자체보다 아세트알데히드에 의한 숙취의 영향이 더 크다. 체내에서 생성된 아세트알데히드는 뇌로 전해져 많은 유해화합물로 바뀌어 맥박 증가나 발한, 홍조, 오심, 구토 등의 증상을 초래할 수 있다.Acetaldehyde, a primary metabolite made by oxidizing alcohol, is a major factor of hangover, and the effects of hangover by acetaldehyde are greater than alcohol itself through toxic effects in the body. Acetaldehyde produced in the body is transmitted to the brain and converted into many harmful compounds that can cause symptoms such as increased pulse, sweating, flushing, nausea and vomiting.
만성적인 알코올 섭취는 소장점막의 손상을 유도하여 vitamin B12, folic acid, thiamin, amino acids(leucine, phenylalanine, glycine, methionine 등), 칼슘(Ca)과 마그네슘(Mg)의 영양소의 소화흡수 및 대사에 부정적인 영향을 미치는 이외에도 일부 영양소의 소변 내 배설을 증가시켜 영양장애를 초래한다. Chronic alcohol intake induces damage to the small intestine mucosa, resulting in digestion and metabolism of nutrients such as vitamin B12, folic acid, thiamin, amino acids (leucine, phenylalanine, glycine, methionine, etc.), calcium (Ca) and magnesium (Mg). In addition to the negative effects, it increases the excretion of some nutrients in the urine, causing malnutrition.
알코올은 체내 대사과정에서 알코올 그 자체 및 알코올 대사물질이 간조직의 손상을 일으키므로 만성적 알코올 섭취는 간에 중성지방을 축적시켜 지방간을 일으키고 과량의 니코틴아미드 아데닌 디뉴클레오티드(NADH)가 생성되어 NADH oxidase의 활성이 높아져 ·OH와 ·O2-와 같은 활성산소(oxygen radical)를 생성함으로써 항산화 시스템에도 영향을 미치게 된다. 그러므로 만성적인 알코올 섭취로 인한 간세포에서 지방의 증가는 곧 지방의 산화억제에 관여하는 셀레늄(Se), 아연(Zn), 망간(Mn), 구리(Cu)의 대사에 영향을 미치기도 하고, 뇨 중 아연, 칼슘, 마그네슘, 망간, 인(P), 철분(Fe) 등의 배설을 증가시킨다. Since alcohol itself and alcohol metabolites cause liver damage in the metabolism of alcohol, chronic alcohol intake causes fatty liver to accumulate triglycerides in the liver, and excess nicotinamide adenine dinucleotide (NADH) is produced to produce NADH oxidase. The activity is increased, producing an oxygen radical such as OH and O 2-which also affects the antioxidant system. Therefore, the increase of fat in liver cells due to chronic alcohol intake also affects the metabolism of selenium (Se), zinc (Zn), manganese (Mn) and copper (Cu), which are involved in fat oxidation inhibition, and urine Heavy zinc, calcium, magnesium, manganese, phosphorus (P), iron (Fe) to increase the excretion.
또한, 자동산화(autoxidation) 반응은 항산화물질에 의해 어느 정도 억제될 수 있는데 셀레늄, 구리, 아연, 망간 등의 무기질은 대표적인 항산화 영양소로 알려져 있으며, 최근 많이 사용되고 있는 페놀계 합성 항산화제인 BHA(butylated hydroxyanisole), BHT(butylated hydroxy toluene) 등은 탁월한 항산화력과 경제성 때문에 널리 이용되고 있으나 생체효소의 활성을 억제하고 암을 유발시키는 등 독성을 가진다고 보고되고 있어, 대부분 사용 규제를 받고 있다. In addition, autoxidation reaction can be inhibited to some extent by antioxidants. Minerals such as selenium, copper, zinc, and manganese are known as representative antioxidant nutrients, and BHA (butylated hydroxyanisole, a synthetic phenol-based antioxidant, which is widely used in recent years) Butylated hydroxy toluene (BHT) is widely used due to its excellent antioxidant power and economical efficiency. However, it has been reported to have toxic effects such as inhibiting the activity of bioenzymes and causing cancer, and most of them are regulated for use.
따라서 부작용이 없고 천연에 존재하는 생물자원으로부터 알코올로 인한 숙취 해소를 효과적으로 억제 및 예방할 수 있는 새로운 물질의 개발이 필요한 실정이다. Therefore, there is a need for the development of a new substance that can effectively suppress and prevent the hangover caused by alcohol from biological resources existing in nature without any side effects.
이에 최근에는 천연물로부터 숙취해소제를 개발하기 위한 연구가 진행되고 있는데, 대한민국공개특허 제2011-0112925호에 숙취해소용 조성물 및 그의 제조 방법으로 숙지황, 감초, 계피, 지구자, 오리나무, 두충, 백출, 천궁, 당귀, 대두, 칡, 진피와 같은 천연 생약재를 사용한 내용이 개시되어 있고, 대한민국등록특허 제1069662호에 감, 곶감 및 마늘을 발효효소를 이용하여 발효, 숙성시킨 숙취해소제에 대한 내용이 개시되어 있으나, 광천수를 숙취 해소를 위한 용도로 개발된 예가 없다. Recently, a research for developing hangover relievers from natural products has been conducted, and as a composition and method for preparing hangover in Korean Patent Laid-Open Publication No. 2011-0112925, ripening sulfur, licorice, cinnamon, earth, alder, tofu, Contents using natural herbal medicines such as Baekchul, Cheongung, Angelica, Soybean, Soybean, and Dermis are disclosed, and Korean Patent No. 1069662 discloses persimmon, dried persimmon, and garlic fermented and aged using fermentation enzymes. Although the contents are disclosed, there is no example developed for the purpose of relieving hangover of mineral water.
본 발명의 목적은 광천수를 함유하는 숙취 해소용 조성물을 제공하는 것이다.It is an object of the present invention to provide a hangover relief composition containing mineral water.
또한, 본 발명의 다른 목적은 본 발명에 따른 상기 광천수를 함유하는 숙취 해소용 조성물을 유효성분으로 함유하는 숙취해소를 위한 건강기능성식품을 제공하는 것이다.In addition, another object of the present invention is to provide a health functional food for hangover relief containing the composition for resolving hangover containing the mineral water according to the present invention as an active ingredient.
상기와 같은 본 발명의 목적을 달성하기 위해서, 본 발명은 대한민국 강원도 강릉시 옥계면 금진3리 92-1번지 해안단구 지역 온천지의 지하 1100m 지점에서 취수한 해양성 광천수를 유효성분으로 포함하는 숙취 해소용 조성물을 제공한다.In order to achieve the object of the present invention as described above, the present invention provides a hangover relieving composition comprising the marine mineral water taken from the 1100m underground of the hot spring area of 92-1 Geumjin 3-ri, Okgye-myeon, Gangneung-si, Gangwon-do, South Korea as an active ingredient to provide.
본 발명의 일실시예에 있어서, 상기 광천수는 미네랄 성분으로서, 칼륨 200~230mg/L, 나트륨 7000~9500mg/L, 칼슘 1400~1700mg/L, 마그네슘 900~1100mg/L, 아연 3~9mg/L, 스트론튬 25~35mg/L, 셀레늄 200~500ug/L, 바나듐 65~75ug/L, 게르마늄 0.5~1.5ug/L, 망간 10~40ug/L, 코발트 1~3ug/L, 티탄 600~950ug/L, 구리 3~9ug/L, 리튬 0.02~0.09mg/L, 염소이온 16000~19500mg/L, 불소이온 1.5~3.0mg/L, 브롬이온 40~56mg/L, 황산이온 3000~4500mg/L, 보론 0.8~1.2mg/L 및 이산화규소 0.5~15mg/L를 함유할 수 있다. In one embodiment of the present invention, the mineral water is a mineral component, potassium 200 ~ 230mg / L, sodium 7000 ~ 9500mg / L, calcium 1400 ~ 1700mg / L, magnesium 900 ~ 1100mg / L, zinc 3 ~ 9mg / L , Strontium 25 ~ 35mg / L, Selenium 200 ~ 500ug / L, Vanadium 65 ~ 75ug / L, Germanium 0.5 ~ 1.5ug / L, Manganese 10 ~ 40ug / L, Cobalt 1-3ug / L, Titanium 600 ~ 950ug / L , Copper 3 ~ 9ug / L, lithium 0.02 ~ 0.09mg / L, chlorine ion 16000 ~ 19500mg / L, fluorine ion 1.5 ~ 3.0mg / L, bromine ion 40 ~ 56mg / L, sulfate ion 3000 ~ 4500mg / L, boron It may contain 0.8-1.2 mg / L and silicon dioxide 0.5-15 mg / L.
본 발명의 일실시예에 있어서, 상기 광천수는 탈염처리 및 증발 농축한 농축물이고, 상기 농축물은 조성물 총 중량을 기준으로 0.5~50%(v/v)로 함유할 수 있다. In one embodiment of the present invention, the mineral water is a desalted and evaporated concentrate, the concentrate may contain 0.5 to 50% (v / v) based on the total weight of the composition.
본 발명의 일실시예에 있어서, 상기 광천수는 혈중 알코올의 농도를 감소시키고, 알코올에 의한 산화적 스트레스를 감소시키는 효과를 가질 수 있다.In one embodiment of the present invention, the mineral water may have the effect of reducing the concentration of alcohol in the blood, reducing the oxidative stress caused by alcohol.
본 발명의 일실시예에 있어서, 상기 광천수는 알코올 분해효소인 간알콜탈수소화효소(ADH) 및 알데히드탈수소화효소(ALDH)의 발현을 증가시키고, 항산화 효소인 GPx(Glutathione peroxidase)의 발현 증가를 통해 숙취 해소 활성을 갖는 것일 수 있다.In one embodiment of the present invention, the mineral water increases the expression of alcohol dehydrogenase hepatic alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), increase the expression of antioxidant enzyme GPx (Glutathione peroxidase) It may be through having a hangover relieving activity.
본 발명의 일실시예에 있어서, 상기 광천수는 알코올로 인해 증가된 체내 총 콜레스테롤 함량 및 중성지방(triglyceride)의 함량을 감소시키는 활성을 가질 수 있다. In one embodiment of the present invention, the mineral water may have an activity of reducing the total cholesterol content and triglyceride content in the body increased due to alcohol.
또한, 본 발명은 본 발명에 따른 상기 조성물을 유효성분으로 함유하는 숙취 해소를 위한 건강기능성식품을 제공한다. In addition, the present invention provides a health functional food for hangover relief containing the composition according to the present invention as an active ingredient.
본 발명에 따른 대한민국 강원도 강릉시 옥계면 금진3리 92-1번지 해안단구 지역 온천지의 지하 1100m 지점에서 취수한 해양성 광천수를 함유한 숙취 해소용 조성물은 알코올 섭취로 인해 체내 증가된 혈중 알코올의 농도를 감소시키고, 알코올에 의한 산화적 스트레스를 감소시키는 효과가 있으며, 간 기능 회복도 우수하여 알코올 섭취 후 효과적인 숙취 해소를 기능성 식품의 제조에 사용될 수 있으며, 나아가 알코올 섭취로 인해 유발될 수 있는 질환의 치료 용도로도 사용할 수 있는 효과가 있다. Hangover composition containing marine mineral water taken from 1100m underground of the hot spring area of Geumjin 3-ri, Okgi-myeon, Gangneung-si, Gangneung-si, Gangwon-do, South Korea according to the present invention reduces the concentration of increased blood alcohol in the body due to alcohol consumption , It has the effect of reducing oxidative stress caused by alcohol, and it also has good recovery of liver function, so it can be used to prepare effective foods after alcohol consumption, and also for the treatment of diseases that can be caused by alcohol consumption. There is also an effect that can be used.
도 1은 알코올 섭취 쥐를 대상으로 식수로 생리식염수를 처리한 군, 광천수군, 비음주군의 혈중 에탄올 농도를 측정한 그래프이다.1 is a graph measuring the blood ethanol concentration of the group treated with saline with drinking water, mineral water group, non-drinking group in alcohol-ingested rats.
도 2는 알코올 섭취 쥐를 대상으로 식수로 생리식염수를 처리한 군, 광천수군에서의 ADH 발현량을 나타낸 그래프이다.Figure 2 is a graph showing the amount of ADH expression in the group treated with physiological saline with drinking water, mineral water group in rats ingesting alcohol.
도 3은 알코올 섭취 쥐를 대상으로 식수로 생리식염수를 처리한 군, 광천수군에서의 ALDH 발현량을 나타낸 그래프이다.3 is a graph showing the amount of ALDH expression in the group treated with physiological saline with drinking water, mineral water group for the rats ingested alcohol.
도 4는 알코올 섭취 쥐를 대상으로 식수로 생리식염수를 처리한 군, 광천수군에서의 항산화 효소인 GPx의 발현량을 나타낸 그래프이다.Figure 4 is a graph showing the expression level of GPx, an antioxidant enzyme in the group treated with saline with drinking water, mineral water in alcohol-ingested rats.
도 5는 알코올 만성 섭취 쥐 모델에서의 처리군별 간 무게를 나타낸 그래프이다.Figure 5 is a graph showing the liver weight by treatment group in the alcohol chronic rat model.
도 6은 알코올 만성 섭취 쥐 모델에서의 처리군별 췌장 무게를 나타낸 그래프이다.Figure 6 is a graph showing the pancreas weight by treatment group in the chronic alcohol intake rat model.
도 7은 알코올 만성 섭취 쥐 모델에서의 간기능검사의 일종인 AST의 측정 결과를 나타낸 그래프이다.Figure 7 is a graph showing the results of the measurement of AST, a type of liver function test in alcoholic rat model of chronic intake.
도 8은 알코올 만성 섭취 쥐 모델에서의 간기능검사의 일종인 ALT의 측정 결과를 나타낸 그래프이다.8 is a graph showing the results of the measurement of ALT, a type of liver function test in a model of chronic alcohol intake of alcohol.
도 9는 알코올 만성 섭취 쥐 모델에서의 간기능검사의 일종인 ALP의 측정 결과를 나타낸 그래프이다.Figure 9 is a graph showing the measurement results of ALP which is a type of liver function test in a model of chronic alcohol intake of alcohol.
도 10은 알코올 만성 섭취 쥐 모델에서의 총콜레스테롤 결과를 나타낸 그래프이다.10 is a graph showing the total cholesterol results in the chronic alcohol intake rat model.
도 11은 알코올 만성 섭취 쥐 모델에서의 중성지방 측정법인 트리글리세라이드 수치를 나타낸 그래프이다.FIG. 11 is a graph showing triglyceride levels, which is a triglyceride measurement method in a rat model of chronic alcohol intake.
도 12는 각 쥐 군으로부터 간 조직을 분리한 후, 헤마토크실렌-에오신 염색을 통해 간 조직 조직 결합 정도를 현미경으로 관찰한 결과를 나타낸 것이다.FIG. 12 shows the results of microscopic observation of liver tissue tissue binding through hematoxylene-eosin staining after separating liver tissue from each rat group.
본 발명은 혈중 알코올 농도를 감소시키고, 알코올에 의한 산화적 스트레스 감소 효과가 우수하며, 간기능을 개선시키는 효과가 우수한 광천수를 유효성분으로 하는 새로운 숙취 해소용 조성물을 제공함에 그 특징이 있으며, 본 발명에 따른 숙취 해소용 조성물에 유효성분으로 함유된 광천수는 대한민국 강원도 강릉시 옥계면 금진3리 92-1번지 해안단구 지역 온천지의 지하 1100m 지점에서 취수한 해양성 광천수라는 점에 특징이 있다. The present invention is characterized in that it provides a new hangover-relieving composition comprising mineral water as an active ingredient which reduces blood alcohol concentration, is excellent in reducing oxidative stress caused by alcohol, and is effective in improving liver function. Mineral water contained as an active ingredient in the hangover composition according to the invention is characterized in that it is marine mineral water taken from the 1100m underground of the hot spring area, 92-1, Geumjin 3-ri, Okgye-myeon, Gangneung-si, Gangwon-do, South Korea.
일반적으로 해수는 표층수와 구별하여 해양 심층수라고 불리우고 있는데, 해양 심층수라는 것은 해수 표면으로부터 200미터 이하의 해수를 일컫는 것으로서, 광합성이 일어나지 않아 식물성장에 필요한 질소, 인, 규산 등의 무기영양소를 많이 포함하고 있으며, 대기나 화학물질에 의한 오염 및 태양균이나 일반세균에 오염되지 않고, 양성 세균수도 적어 물리적 청정성도 매우 뛰어난 것으로 알려져 있다. In general, seawater is called deep seawater, which is distinguished from surface water, and deep sea water refers to seawater 200 meters or less from the surface of seawater. It contains many inorganic nutrients such as nitrogen, phosphorus, and silicic acid needed for plant growth because photosynthesis does not occur. It is known that it is not polluted by air or chemicals and contaminated with solar bacteria or general bacteria, and has a small number of benign bacteria, so that the physical cleanliness is excellent.
또한, 일년 내내 저온으로 그 변화가 적고 안정된 낮은 수온성을 갖으며, 필수 미량원소나 다양한 미네랄 성분이 균형있게 포함되어 있어 용존되어 있는 금속이온들의 작용으로 활성 산소에 대한 탁월한 소거작용 효과 등 체내 유용한 특성을 가지고 있다. In addition, its temperature is low all year round, its stability is low, and its water temperature is stable. It contains essential trace elements and various mineral components in a balanced manner, and it is useful in the body such as excellent scavenging effect on active oxygen due to the action of dissolved metal ions. Has characteristics.
따라서 해양 오염이 심각한 현재의 상태에서 표층수는 식음료 등 인체에 사용하기에 적합하지 않으나, 해양심층수는 표면층에 비하여 생균수가 그다지 많지 않을뿐더러 병원성 생물이 거의 포함되어 있지 않기 때문에 의료용 및 식음료로 선택하는 경우에 안전성이 지극히 높다고 할 수 있다. Therefore, in the current state of serious marine pollution, surface water is not suitable for use in the human body such as food and beverage, but since deep sea water is not much more viable than the surface layer and contains little pathogenic organisms, it is selected as medical and food and beverage. The safety is extremely high.
한편, 최근 사회의 다양화 및 경제성장과 더불어 사교 및 스트레스 해소 목적으로 현대인의 알코올 섭취량이 증가하고 있어 과음과 관련된 숙취나 알코올 중독과 같은 사회문제가 발생하여 경제적 및 사회적 비용 손실을 초래한다. On the other hand, in recent years, alcohol consumption of modern man is increasing for the purpose of socializing and socialization along with social diversification and economic growth, resulting in social problems such as hangover and alcoholism related to heavy drinking, resulting in economic and social cost loss.
이에 본 발명자들은 해양 광천수를 이용하여 숙취 해소를 할 수 있는 조성물을 개발하던 중, 대한민국 강원도 강릉시 옥계면 금진3리 92-1번지 해안단구 지역 온천지의 지하 1100m 암반층에서 용출되는 해양 심층수인 해양성 광천수가 혈중 알코올 농도를 감소시키고, 알코올에 의한 산화적스트레스 감소 효과가 우수하며, 간기능을 개선시키는 효과가 우수하다는 것을 확인하여, 상기 광천수를 숙취 해소용 조성물로 사용하기에 아주 적절하다는 사실을 확인하였다. Accordingly, the present inventors are developing a composition that can resolve hangover using marine mineral water. By reducing the alcohol concentration, the effect of reducing the oxidative stress by alcohol, and the effect of improving the liver function was confirmed, it was confirmed that the mineral water is very suitable for use as a hangover composition.
즉, 본 발명의 일실시예에 따르면, 급성 알코올 중독 마우스 모델을 대상으로 본 발명의 광천수를 섭취하게 한 마우스와 광천수 대신 생리식염수를 섭취하게 한 마우스로부터 혈중 에탄올 농도를 측정한 결과, 본 발명의 광천수를 섭취하게 한 마우스의 경우, 대조군 마우스에 비해 혈중 알코올 농도가 현저하게 감소되는 것으로 나타났다(도 1 참조).That is, according to an embodiment of the present invention, the blood ethanol concentration was measured from a mouse ingesting the mineral water of the present invention and a mouse receiving the saline instead of the mineral water in the mouse model of acute alcoholism, Mice fed with mineral water showed a significant decrease in blood alcohol concentrations compared to control mice (see FIG. 1).
본 발명의 다른 일실시예에서는 본 발명의 광천수가 효과적으로 숙취 해소를 할 수 있는지를 확인하기 위해 알코올 분해효소로 알려진 알콜탈수소화효소(ADH)와 알데히드탈수소화효소(ALDH)활성을 앞서 실험한 알코올 중독 마우스 모델을 대상으로 측정한 결과, 본 발명의 광천수를 섭취하게 한 군이 광천수를 섭취하지 않은 대조군에 비해 ALDH mRNA 발현량 및 ADH mRNA 발현량을 월등히 증가시킨다는 사실을 알 수 있었으며, 상기 유전자들의 발현 증가는 처리한 광천수의 농도에 비례하는 것으로 나타났다(도 2 및 도 3 참조).In another embodiment of the present invention, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activity known as alcohol degrading enzymes to confirm whether the mineral water of the present invention can effectively resolve hangover alcohol As a result of measuring the poisoned mouse model, it was found that the group ingesting mineral water of the present invention significantly increased the expression level of ALDH mRNA and ADH mRNA in comparison with the control group that did not consume mineral water. Increased expression was shown to be proportional to the concentration of treated mineral water (see FIGS. 2 and 3).
또한, 본 발명의 다른 일실시예에서는 본 발명의 광천수가 알코올에 의한 체내 산화작용을 억제하는 항산화 활성이 있는지를 조사하기 위해, 셀레늄과 관련된 대표적인 항산화 효소로 알려진 글루타치온 퍼옥시다아제(GPx)의 활성을 분석하였는데, 그 결과, 광천수를 섭취하게 한 군이 대조군에 비해 항산화 효소인 GPx mRNA의 발현양이 현저하게 증가한 것으로 나타났다(도 4 참조).In addition, in another embodiment of the present invention, to investigate whether the mineral water of the present invention has an antioxidant activity that inhibits the oxidation of the body by alcohol, glutathione peroxidase (GPx) known as a representative antioxidant enzyme associated with selenium As a result, it was shown that the group of mineral water ingested significantly increased the expression level of the antioxidant enzyme GPx mRNA compared to the control group (see FIG. 4).
따라서 이러한 결과를 통해 본 발명자들은 본 발명의 광천수가 알코올 분해 효소의 발현을 증가시킬 수 있으며, 알코올에 의한 체내 산화 작용을 억제하는 항산화 활성을 가지고 있어 숙취 해소를 위한 용도로 사용할 수 있다는 것을 알 수 있었다. Therefore, the present inventors can see that the mineral water of the present invention can increase the expression of alcohol degrading enzymes, and has an antioxidant activity that inhibits the oxidative action of the body by alcohol, so that the present invention can be used for hangover relief. there was.
나아가, 본 발명자들은 본 발명의 광천수가 알코올에 의한 간 독성을 개선시킬 수 있는 효과가 있는지 확인하기 위해, 간기능 검사의 지표로 사용되고 있는 AST, ALT 및 ALP의 발현 정도를 측정하였는데, 본 발명의 광천수는 알코올로 인해 간에서 발현이 증가되는 상기 3가지의 효소를 억제하는 활성이 있다는 사실을 알 수 있었고, AST, ALT 및 ALP의 발현억제 정도는 처리한 광천수의 농도에 비례하는 것으로 나타났다(도 7 내지 9 참조). Furthermore, the present inventors measured the expression levels of AST, ALT, and ALP, which are used as indicators of liver function tests, to determine whether the mineral water of the present invention can improve liver toxicity by alcohol. It was found that mineral water has the activity of inhibiting the three enzymes that increase the expression in the liver due to alcohol, and the degree of inhibition of expression of AST, ALT and ALP was proportional to the concentration of treated mineral water (Fig. 7 to 9).
상기 ALT는 알라닌 아미노기 전이 효소로서 간세포 내의 효소이며, 혈청글루탐피루빈산아미노기전이효소(Serum Glutamic Pyruvate Transaminase, SGPT) 또는 알라닌아미노기전달효소(ALAT)로 불리기도 한다. 세포 손상 시 혈액 내로 방출된 효소의 양을 측정하여 간 손상 정도를 분석할 수 있는데, ALT는 급성 간 손상에서 극적으로 그 수치가 상승하는 것으로 알려져 있다. The ALT is an alanine aminotransferase and an enzyme in hepatocytes, also called serum glutamic pyruvate aminotransferase (SGPT) or alanine aminotransferase (ALAT). The degree of liver damage can be analyzed by measuring the amount of enzyme released into the blood during cell damage. ALT is known to increase dramatically in acute liver injury.
또한, AST는 아스파르트산염 아미노기 전이 효소 또는 아스파라진산 아미노전이효소(aspartate aminotransferase, ASAT)로도 불리며, 간 조직의 세포와 관련된 또 다른 효소라는 점에서 ALT와 유사하다. 이 효소 또한 급성 간 손상이 있을 때에 발현이 증가하는 것으로 알려져 있다. AST is also called aspartate aminotransferase or aspartate aminotransferase (ASAT) and is similar to ALT in that it is another enzyme associated with cells of liver tissue. This enzyme is also known to increase expression in acute liver injury.
ALP(알칼라인 포스파타아제;Alkaline phosphatase)는 간의 담관 안을 싸고 있는 세포 내부의 효소로서, ALP의 혈장내 농도는 담관의 폐색, 간 내부의 담즙 분비 중지 또는 침투성 세균으로 인한 질병과 함께 상승하는 것으로 알려져 있다.ALP (alkaline phosphatase) is an enzyme inside the cells that surround the bile ducts, and plasma concentrations of ALP are known to rise with obstruction of the bile ducts, stopping bile secretion inside the liver, or diseases caused by invasive bacteria. have.
또한, 지속적인 알코올 중독은 간 대사 능력을 떨어뜨리고 특히 지방 분해 능력을 저하하여 지방간을 유발시키는 것으로 알려져 있는데, 이에 본 발명자들은 본 발명에 따른 광천수가 체내 총콜레스테롤 및 트리글리세라이드의 농도를 감소시키는 효과가 있는지 분석하였는데, 그 결과 총콜레스테롤 및 중성지방인 트리글리세라이드가 본 발명에 따른 광천수에 의해 효과적으로 저해되고 있음을 확인할 수 있었다(도 10 및 도 11 참조).In addition, persistent alcoholism is known to cause liver metabolism by lowering liver metabolism, and in particular, by lowering lipolytic capacity. Therefore, the present inventors have found that mineral water according to the present invention has an effect of reducing the concentration of total cholesterol and triglycerides in the body. As a result, it was confirmed that the total cholesterol and triglycerides, which are triglycerides, were effectively inhibited by the mineral water according to the present invention (see FIGS. 10 and 11).
뿐만 아니라 본 발명의 다른 일실시예를 통해 본 발명의 광천수가 알코올로 인한 간 손상을 회복시키는 작용이 있다는 사실을 알코올 중독 마우스 동물 모델의 간조직 검사를 통해 확인할 수 있었다(도 12 참조).In addition, through another embodiment of the present invention, it was confirmed that the mineral water of the present invention has an effect of restoring liver damage caused by alcohol through liver tissue examination of an alcoholic mouse animal model (see FIG. 12).
이상과 같은 실험 결과들을 통해 본 발명자들은 본 발명에서 제공하고 있는 광천수가 알코올 섭취로 인해 증가되는 혈중 알코올 농도를 알코올 분해 효소의 발현 촉진을 통해 효과적으로 감소시킬 수 있으며, 알코올에 의한 산화적 스트레스도 억제할 수 있는 효과가 있고, 간 조직에 축적된 총 콜레스테롤 및 중성지방의 함량을 감소시키며, 간 기능을 개선시키는 효과가 우수하다는 사실을 확인함에 따라 궁극적으로 본 발명의 광천수를 숙취 해소를 위한 용도로 사용할 수 있다는 사실을 알 수 있었다. Through the above experimental results, the present inventors can effectively reduce the blood alcohol concentration, which is increased by the mineral water provided by the present invention, by promoting the expression of alcohol degrading enzymes, and also inhibits the oxidative stress caused by alcohol. It is effective to reduce the amount of total cholesterol and triglycerides accumulated in liver tissue, and to improve the function of the liver, and as a result, the mineral water of the present invention is ultimately used to relieve hangover. You can see that it can be used.
그러므로 본 발명은 대한민국 강원도 강릉시 옥계면 금진3리 92-1번지 해안단구 지역 온천지의 지하 1100m 지점에서 취수한 해양성 광천수를 유효성분으로 포함하는 숙취 해소용 조성물을 제공할 수 있다.Therefore, the present invention can provide a hangover relief composition comprising marine mineral water taken at 1100m underground from the hot spring area of 92-1, Geumjin 3-ri, Okgye-myeon, Gangneung-si, Gangwon-do, Korea.
또한, 본 발명의 조성물에 유효성분으로 포함되는 상기 광천수는 미네랄 성분으로서, 칼륨 200~230mg/L, 나트륨 7000~9500mg/L, 칼슘 1400~1700mg/L, 마그네슘 900~1100mg/L, 아연 3~9mg/L, 스트론튬 25~35mg/L, 셀레늄 200~500ug/L, 바나듐 65~75ug/L, 게르마늄 0.5~1.5ug/L, 망간 10~40ug/L, 코발트 1~3ug/L, 티탄 600~950ug/L, 구리 3~9ug/L, 리튬 0.02~0.09mg/L, 염소이온 16000~19500mg/L, 불소이온 1.5~3.0mg/L, 브롬이온 40~56mg/L, 황산이온 3000~4500mg/L, 보론 0.8~1.2mg/L 및 이산화규소 0.5~15mg/L를 함유한다.In addition, the mineral water contained in the composition of the present invention as an active ingredient is a mineral component, potassium 200 ~ 230mg / L, sodium 7000 ~ 9500mg / L, calcium 1400 ~ 1700mg / L, magnesium 900 ~ 1100mg / L, zinc 3 ~ 9mg / L, Strontium 25 ~ 35mg / L, Selenium 200 ~ 500ug / L, Vanadium 65 ~ 75ug / L, Germanium 0.5 ~ 1.5ug / L, Manganese 10-40ug / L, Cobalt 1-3ug / L, Titanium 600 ~ 950ug / L, copper 3 ~ 9ug / L, lithium 0.02 ~ 0.09mg / L, chlorine ion 16000 ~ 19500mg / L, fluorine ion 1.5 ~ 3.0mg / L, bromine ion 40 ~ 56mg / L, sulfate ion 3000 ~ 4500mg / L, 0.8-1.2 mg / L boron and 0.5-15 mg / L silicon dioxide.
이러한 성분 분석은 한국과학기술연구원(KIST), 한국기초과학지원연구원(KBSI) 및 한국지질자원연구원(KIGAM)에 의뢰한 본 발명에 따른 광천수의 미네랄 성분 분석 결과로서, 이러한 상기 미네랄 성분 구성을 지닌 광천수는 지금까지 알려진 바 없는 신규한 것이다.This component analysis is a result of the mineral component analysis of the mineral water according to the present invention commissioned by the Korea Institute of Science and Technology (KIST), the Korea Basic Science Institute (KBSI) and the Korea Institute of Geoscience and Mineral Resources (KIGAM). Mineral waters are new to date.
또한, 본 발명에서 상기 광천수는 대한민국 강원도 강릉시 옥계면 금진3리 92-1번지 해안단구 지역 온천지의 지하 1100m 지점에서 취수한 해양성 광천수 자체를 사용할 수 있고, 광천수 자체를 사용할 경우 상기 광천수는 조성물 총 중량을 기준으로 50%(v/v)~100%(v/v)의 양으로 사용할 수 있으며, 또는 농축물로 제조하여 사용할 수 있고, 농축물로 사용할 경우에는 조성물 총 중량을 기준으로 0.5~50%(v/v)의 양으로 함유하여 사용할 수 있다. In addition, in the present invention, the mineral water may use the marine mineral water itself taken from the 1100m underground of the hot spring area, 92-1, Geumjin 3-ri, Okgye-myeon, Gangneung-si, Gangneung-si, Gangwon-do, South Korea, the mineral water is the total weight of the composition It can be used in an amount of 50% (v / v) to 100% (v / v) as a standard, or can be prepared and used as a concentrate, and when used as a concentrate, 0.5 to 50% based on the total weight of the composition It can be contained and used in the quantity of (v / v).
상기 농축물은 본 발명에 따른 광천수를 증발, 농축 및 건조시켜 얻은 고형분일 수 있고, 또는 상기 광천수를 자연건조로 증발시키거나, 인공적으로 탈염 및 건조(또는 증발)시켜 얻은 농축액일 수도 있다. The concentrate may be a solid obtained by evaporating, concentrating and drying the mineral water according to the present invention, or may be a concentrate obtained by evaporating the mineral water by natural drying or by artificially desalting and drying (or evaporating) the mineral water.
구체적으로 본 발명의 따른 상기 광천수의 농축물은 하기와 같은 방법으로 제조될 수 있다. 우선 원수(原水)를 채수한 후, 필터링하는 여과 단계를 거친다. 이때 상기 여과단계는 여러 차례의 여과 단계를 거칠 수 있는데, 본 발명에서는 4단계의 여과 과정을 수행하였다. 즉, 1단계 여과는 10um 이상의 부유물을 필터링하여 제거하였고, 이후 2단계 여과로서 다시 5um 이상의 부유물을 필터링하여 제거한 다음, 3단계 여과로서 1um 이상의 부유물을 필터링하여 제거하였다. 이후 UV를 통해 살균 처리한 다음, 4단계 여과과정으로 0.2um 이상의 부유물을 필터링하여 제거하였다. 이렇게 여과 과정을 거쳐 수득한 수득물은 탈염 단계 및 건조 과정을 수행할 수 있으며, 탈염은 이에 제한되는 것은 아니나 전기투석기를 이용할 수 있다. Specifically, the concentrate of the mineral water according to the present invention may be prepared by the following method. First, raw water is collected and then filtered. At this time, the filtration step may be subjected to a plurality of filtration steps, in the present invention was carried out a four-step filtration process. That is, the first stage filtration was removed by filtering the suspended matter more than 10um, and then removed by filtering the suspended matter more than 5um again as a second stage filtration, and then removed by filtering the suspended matter above 1um as a three-step filtration. After sterilization through UV, the suspended solids filtered by 0.2um or more by a four-step filtration process. Thus obtained product obtained by the filtration process can be carried out a desalting step and drying process, the desalination is not limited thereto, but may be used an electrodialysis machine.
이후, 자연건조나 인공건조기를 이용한 건조단계를 거쳐 탈염 파우더를 제조한다. 본 발명의 일실시예에서는 상기 건조를 위해 진공동결 건조기를 사용하였다. 마지막 단계에서는 탈염 파우더를 분쇄하여 포장할 수 있다.Thereafter, desalination powder is prepared through a drying step using natural drying or an artificial dryer. In one embodiment of the present invention, a vacuum freeze dryer was used for the drying. In the final step, the desalted powder can be ground and packed.
또한, 본 발명에 따른 광천수의 농축물의 제조는 원수를 채수한 후 건조과정을 통해 먼저 농축과정을 수행할 수 있고, 이후 여과 단계를 수행할 수 있다. In addition, the preparation of the concentrate of the mineral water according to the present invention may be carried out first through the drying process after collecting the raw water, and then performing the filtration step.
본 발명의 조성물은 혈중 알코올 농도를 낮춰 주는 등의 숙취 해소를 위한 약학적 조성물로 사용될 수 있으며, 본 발명에 따른 광천수를 농축하여 사용할 경우, 상기 조성물 총 중량에 대하여 0.5~50중량%로 포함할 수 있다. The composition of the present invention may be used as a pharmaceutical composition for relieving hangover, such as lowering blood alcohol concentration, and when used in concentrated mineral water according to the present invention, it may include 0.5 to 50% by weight relative to the total weight of the composition. Can be.
또한, 본 발명에 따른 상기 숙취 해소를 위한 조성물은 천연 해양 심층수를 원료로 하는 바 기존의 화학적 제제보다 안전성이 높아, 약물에 대한 별다른 독성 및 부작용도 없어 장기간 복용 시에도 안심하고 사용할 수 있다.In addition, the composition for resolving hangover according to the present invention is safer than conventional chemical preparations using natural deep sea water as a raw material, and there are no toxic and side effects for drugs, so it can be used with confidence even during long-term use.
따라서 본 발명의 조성물은 알코올에 의해 발생하는 질환의 예방 또는 치료를 목적으로 하는 식품에 첨가할 수 있으므로, 알코올로 인해 발생하는 질환 증상의 예방 및 개선을 위한 식품용 조성물 또는 숙취 해소용 건강기능성 식품 조성물로 사용할 수 있다. Therefore, the composition of the present invention can be added to a food for the purpose of preventing or treating a disease caused by alcohol, a composition for food or a health functional food for hangover relief for the prevention and improvement of disease symptoms caused by alcohol It can be used as a composition.
알코올로 인해 발생하는 질환 증상의 예방 및 개선에 효과가 있는 식품, 예컨대, 식품의 주원료, 부원료, 식품 첨가제, 기능성 식품 또는 음료로 용이하게 활용할 수 있다.Food, which is effective in preventing and ameliorating disease symptoms caused by alcohol, for example, it can be easily utilized as a main ingredient, side ingredients, food additives, functional food or beverage of food.
본 발명에서 상기 "식품"이란, 영양소를 한 가지 또는 그 이상 함유하고 있는 천연물 또는 가공품을 의미하며, 바람직하게는 어느 정도의 가공 공정을 거쳐 직접 먹을 수 있는 상태가 된 것을 의미하며, 통상적인 의미로서, 식품, 식품 첨가제, 기능성 식품 및 음료를 모두 포함하는 것을 말한다.In the present invention, the "food" means a natural product or processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through a certain processing step, and a general meaning As used herein, it refers to food, food additives, functional foods and beverages.
본 발명에 따른 조성물을 첨가할 수 있는 식품으로는 예를 들어, 각종 식품류, 음료, 껌, 차, 비타민 복합제, 기능성 식품 등이 있다. 추가로, 본원발명에서 식품에는 특수영양식품(예, 조제유류, 영,유아식 등), 식육가공품, 어육제품, 두부류, 묵류, 면류(예, 라면류, 국수류 등), 빵류, 건강보조식품, 조미식품(예, 간장, 된장, 고추장, 혼합장 등), 소스류, 과자류(예, 스넥류), 캔디류, 쵸코렛류, 껌류, 아이스크림류, 유가공품(예, 발효유, 치즈 등), 기타 가공식품, 김치, 절임식품(각종 김치류, 장아찌 등), 음료(예, 과실 음료, 채소류 음료, 두유류, 발효음료류 등), 천연조미료(예, 라면 스프 등)을 포함하나 이에 한정되지 않는다. 상기 식품, 음료 또는 식품첨가제는 통상의 제조방법으로 제조될 수 있다.Foods to which the composition according to the present invention can be added include, for example, various foods, beverages, gums, teas, vitamin complexes, functional foods, and the like. In addition, in the present invention, food includes special nutritional products (e.g., formulated milk, young, infant food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g. ramen, noodles, etc.), bread, health supplements, seasonings. Foods (e.g. soy sauce, miso, red pepper paste, mixed soy sauce), sauces, confectionery (e.g. snacks), candy, chocolates, gums, ice creams, dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, Pickled foods (various kimchi, pickles, etc.), beverages (e.g., fruit drinks, vegetable drinks, soy milk, fermented beverages, etc.), natural seasonings (e.g. ramen soup, etc.) are not limited thereto. The food, beverage or food additives may be prepared by a conventional manufacturing method.
또한, 상기 "기능성 식품"이란 식품에 물리적, 생화학적, 생물 공학적 수법 등을 이용하여 해당 식품의 기능을 특정 목적에 작용, 발현하도록 부가가치를 부여한 식품군이나 식품 조성이 갖는 생체방어리듬조절, 질병방지와 회복 등에 관한 체내조절기능을 생체에 대하여 충분히 발현하도록 설계하여 가공한 식품을 의미하며, 구체적으로는 건강 기능성 식품일 수 있다. 상기 기능성 식품에는 식품학적으로 허용 가능한 식품 보조 첨가제를 포함할 수 있으며, 기능성 식품의 제조에 통상적으로 사용되는 적절한 담체, 부형제 및 희석제를 더욱 포함할 수 있다.In addition, the term "functional food" refers to the control of biological defense rhythms and disease prevention of food groups or food compositions that have added value to the food by using physical, biochemical, or biotechnological techniques to act and express the function of the food for a specific purpose. It means a food that is designed and processed to fully express the body's regulatory function regarding recovery and the like, and specifically, it may be a health functional food. The functional food may include food acceptable food additives, and may further include appropriate carriers, excipients and diluents commonly used in the manufacture of functional foods.
또한, 본원발명에서 상기 "음료"란 갈증을 해소하거나 맛을 즐기기 위하여 마시는 것의 총칭을 의미하며 기능성 음료를 포함한다. 상기 음료는 지시된 비율로 필수 성분으로서 본 발명에 따른 상기 숙취 해소용 조성물을 포함하는 것 외에 다른 성분에는 특별한 제한이 없으며 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다.In addition, in the present invention, the "beverage" refers to a generic term for drinking to quench thirst or to enjoy a taste and includes a functional drink. The beverage is not particularly limited in addition to the composition for eliminating hangover according to the present invention as an essential ingredient in the ratio indicated, and may contain various flavors or natural carbohydrates, etc. as an additional ingredient like a normal beverage. Can be.
나아가 상기 기술한 것 이외에 본원발명의 조성물을 함유하는 식품은 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 충진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있으며, 상기 성분은 독립적으로 또는 조합하여 사용할 수 있다.Furthermore, foods containing the composition of the present invention, in addition to those described above, include various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavoring agents, colorants and fillers (such as cheese and chocolate), and pectic acid. And salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like, which components may be independently or in combination Can be used.
본원발명의 조성물을 함유하는 식품에 있어서, 상기 본 발명에 따른 조성물의 양은 전체 식품 중량의 0.001중량% 내지 90중량%로 포함할 수 있으며, 바람직하게는 0.1중량% 내지 40중량%로 포함할 수 있고, 음료의 경우, 100ml를 기준으로 0.001g 내지 2g, 바람직하게는 0.01g 내지 0.1g의 비율로 포함할 수 있으나, 건강 및 위생을 목적으로 하거나 건강 조절을 목적으로 하는 장기간 섭취의 경우에는 상기 범위 이하일 수 있으며, 유효성분은 안전성 면에서 아무런 문제가 없기 때문에 상기 범위 이상의 양으로 사용될 수 있으므로 상기 범위에 한정되는 것은 아니다.In the food containing the composition of the present invention, the amount of the composition according to the present invention may comprise from 0.001% to 90% by weight of the total food weight, preferably from 0.1% to 40% by weight. In the case of a beverage, it may be included in a ratio of 0.001g to 2g, preferably 0.01g to 0.1g based on 100ml, in the case of long-term intake for health and hygiene purposes or health control purposes It may be less than the range, the active ingredient is not limited to the above range because it may be used in an amount above the range because there is no problem in terms of safety.
그러므로 본 발명은 본 발명에 따른 광천수 또는 광천수 농축물을 유효성분으로 함유하는 숙취 해소를 위한 건강기능식품을 제공할 수 있으며, 상기 식품의 형태는 이에 제한되지는 않으나, 분말, 과립, 정제, 캡슐 또는 음료 형태일 수 있다.Therefore, the present invention can provide a health functional food for relieving hangover containing mineral water or mineral water concentrate according to the present invention as an active ingredient, but the form of the food is not limited thereto, but the powder, granule, tablet, capsule Or in the form of a drink.
이하, 본 발명을 하기 실시예 및 실험예에 의해 상세히 설명한다. 단, 하기 실시예 및 실험예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실험예에 의해 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples. However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited by the following Experimental Examples.
<실시예 1><Example 1>
본 발명에 따른 광천수 채취 및 농축물 제조Mineral water extraction and concentrate preparation according to the present invention
본 발명자들은 숙취 해소에 효능이 있는 광천수를 채취하기 위해 원수(原水)인 강원도 강릉시 옥계면 금진리 해변 해안단구 지역의 지하 110m 암반층에서 취수한 온천수를 채취하여 0.25 마이크로 미터의 라인필터를 이용하여 필터링하여 오염물질을 제거한 후, 전기투석기(전압: 5.5~7.5V, 전극액: 3% Na2SO4, CJTTS-2-10 ED, 창조테크노)를 이용하여 탈염하였다. 탈염 과정을 마친 후, 여과하고 진공동결 건조기(온도: -40~30℃, 진공도: 5mTorr)를 이용하여 건조 과정을 수행하였고, 이러한 과정으로 제조된 본 발명의 광천수 농축물을 하기와 같은 실험에 사용하였다.The present inventors collected the hot spring water taken from the 110m underground bedrock of the Geumjin-ri beach coastal area of Okgye-myeon, Gangneung-si, Gangneung-si, Gangwon-do, to collect the mineral water that is effective in resolving hangovers. After removing the material, it was desalted using an electrodialysis machine (voltage: 5.5-7.5 V, electrode solution: 3% Na 2 SO 4 , CJTTS-2-10 ED, Creative Techno). After the desalination process was filtered and dried using a vacuum freeze dryer (temperature: -40 ~ 30 ℃, vacuum degree: 5mTorr), the mineral water concentrate of the present invention prepared by this process in the following experiment Used.
<실시예 2><Example 2>
알코올 섭취 쥐 모델 제작Production of Alcohol Intake Rat Model
상기 실시예 1에 따른 본 발명의 광천수가 숙취 해소 작용을 하는지 확인하기 위한 실험을 위해 하기와 같이 알코올을 섭취한 동물 모델을 제작하였다. 생후 9주된 400gram의 건강한 수컷 Sprague Dawley rat을 대상으로 병원균이 없는 환경에서 2주 이상 사육하였고, 멸균된 표준 쥐 사료(Ralston Purina, St. Louis, MO)를 섭취하게 하였으며 동물 실험은 가톨릭대학교 IACUC(Institutional Animal Care and Use Committee)의 승인 하에서 이루어졌다. 이와 같이 사육된 마우스를 대상으로 급성 알코올 중독 마우스 및 만성 알코올 중독 마우스를 만들었는데, 먼저 급성 알코올 중독 마우스는 실험 수행 18시간 이전부터 금식하도록 하였고, 하기 표 1과 같이 3가지 동물 실험군을 만들어서 하기 실험들을 수행하였다.For experiments to determine whether the mineral water of the present invention according to Example 1 has a hangover resolution, an animal model ingesting alcohol was prepared as follows. 400 grams of healthy male Sprague Dawley rats, 9 weeks old, were bred for 2 weeks or more in a pathogen-free environment and were fed sterile standard rat feed (Ralston Purina, St. Louis, MO). Under the approval of the Institutional Animal Care and Use Committee. Thus, acute alcoholism mice and chronic alcoholism mice were made for the bred mice. First, the acute alcoholism mice were fasted for 18 hours before the experiment, and the following experiment was made by making three animal test groups as shown in Table 1 below. Were performed.
표 1 급성 알콜 중독 마우스 모델 군
1(대조군) 비음주 군으로 아무것도 처리하지 않은 정상마우스 군
2 알코올 섭취 이전에 생리식염수 1ml를 섭취시킨 후, 알코올 섭취시킨 군
3 본 발명의 광천수 1ml를 섭취하게 한 후, 알코올을 섭취시킨 군
Table 1 Acute Alcoholism Mouse Model Group
1 (control) Normal mouse group that treated nothing with non-drinking group
2 Ingestion of 1 ml of saline solution before alcohol consumption
3 After ingesting 1ml mineral water of the present invention, the group ingested alcohol
이때 상기 마우스를 대상으로 20%의 알코올을 마우스 한 마리당 2.5ml의 양으로 섭취하게 하였다.At this time, the mice were ingested with 20% alcohol in an amount of 2.5 ml per mouse.
<실시예 3><Example 3>
본 발명에 따른 광천수의 숙취 해소 활성 분석Hangover Relief Activity Analysis of Mineral Waters According to the Present Invention
<3-1> 혈중 에탄올 농도 측정<3-1> Blood ethanol concentration measurement
상기 실시예 2에서 제작한 동물 실험군들을 대상으로 에탄올을 섭취하게 한 시점으로부터 1시간째, 3시간째, 5시간째 및 7시간째에 정맥으로부터 혈액을 채취하여, 3000rpm에서 10분간 원심분리한 후, 상등액을 분리하고 혈중 알코올 농도를 에탄올정량 키트(ethanol assay kit)(BioVision Cat:K620-100, USA)를 사용하여 흡광도 570nm에서 측정하였다. Blood was collected from the veins at 1 hour, 3 hours, 5 hours and 7 hours from the time point at which the ethanol was ingested from the animal test groups prepared in Example 2, and then centrifuged at 3000 rpm for 10 minutes. The supernatant was separated and the blood alcohol concentration was measured at 570 nm with absorbance using an ethanol assay kit (BioVision Cat: K620-100, USA).
그 결과, 도 1에서 나타낸 바와 같이, 혈중 알코올 농도는 알코올 섭취 후 3시간이 경과한 시점에 최고치를 보이는 것으로 나타났으며, 알코올 섭취 이전에 생리식염수를 섭취하게 한 군과 본 발명의 광천수를 섭취하게 한 군을 비교한 결과에서는 광천수 섭취 군이 생리식염수 섭취 군에 비해 혈중 알코올 농도를 현저하게 감소시키는 것으로 나타났다. As a result, as shown in Figure 1, the blood alcohol concentration was shown to show the highest point at 3 hours after the intake of alcohol, ingested the saline prior to alcohol intake and the mineral water of the present invention As a result of comparing the groups, the mineral water intake group significantly decreased blood alcohol concentration compared to the physiological saline intake group.
<3-2> ADH, ALDH 활성 측정<3-2> ADH, ALDH activity measurement
본 발명의 광천수가 숙취 해소 활성을 가지는가를 알아보기 위하여 상기 실시예 <3-1>의 실험군에서 에탄올 섭취 후 7시간이 경과한 다음, 마지막 채혈이 완료되면 각 마우스들을 치사시키고 간 조직을 분리한 다음, 상기 조직으로부터 RNA를 추출하고, 간 조직에 발현된 간알콜탈수소화효소(ADH)와 알데히드탈수소화효소(ALDH)의 발현 정도를 분석하기 위해 상기 유전자들에게 특이적인 프라이머를 사용하여 RT-PCR을 수행하였으며, 사용한 프라이머들의 염기서열을 다음에 기재된 바와 같다.  In order to determine whether the mineral water of the present invention has hangover-relieving activity, after 7 hours after ethanol ingestion in the experimental group of Example <3-1>, each mouse was lethal and separated from liver tissue when the final blood collection was completed. Next, RNA was extracted from the tissue, and RT-using primers specific for the genes to analyze the expression levels of hepatic alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) expressed in liver tissue. PCR was performed and the base sequences of the primers used are as described below.
<프라이머 서열>Primer Sequence
ADH 정방향 프라이머; 5'-ctgtaaagcagcaggagcag-3ADH forward primer; 5'-ctgtaaagcagcaggagcag-3
ADH 역방향 프라이머; 5'-tcctacgacgacgcttacac -3'ADH reverse primer; 5'-tcctacgacgacgcttacac -3 '
ALDH 정방향 프라이머; 5'-gatcaacacaggtgatttattcc-3'ALDH forward primer; 5'-gatcaacacaggtgatttattcc-3 '
ALDH 역방향 프라이머; 5'-tgagggccttgatttattcc-3'ALDH reverse primer; 5'-tgagggccttgatttattcc-3 '
그 결과, 도 2 및 도 3에 나타낸 바와 같이, 알코올 섭취 이전에 생리식염수를 섭취하게 한 군에 비해 본 발명의 광천수를 섭취하게 한 군이 알콜 분해 효소들인 간알콜탈수소화효소(ADH)와 알데히드탈수소화효소(ALDH)가 간 조직내에서 많이 발현되어 있는 것으로 나타났다.As a result, as shown in Figures 2 and 3, compared to the group of the physiological saline intake before alcohol intake group, the group ingesting mineral water of the present invention alcohol dehydrogenases (ADH) and aldehydes Dehydrogenase (ALDH) was found to be highly expressed in liver tissue.
<3-3> GPx 효소의 활성 분석<3-3> Activity analysis of GPx enzyme
나아가 본 발명자들은 알코올 섭취 시 발생되는 것으로 알려진 산화적 스트레스에 대해 본 발명의 광천수가 이를 억제하는 활성이 있는지 확인하기 위해 상기 실시예 <3-2>에서 수득한 간 조직의 RNA를 대상으로 항산화 효소로 알려진 글루타치온 퍼옥시다아제(GPx)의 발현 정도를 RT-PCR을 통해 분석하였다. 이때 사용한 GPx의 프라이머의 염기서열은 다음과 같다.Furthermore, the present inventors have conducted antioxidant enzymes targeting RNA of liver tissues obtained in Example <3-2> to check whether the mineral water of the present invention inhibits the oxidative stress known to occur when alcohol is ingested. The expression level of glutathione peroxidase (GPx) known as was analyzed by RT-PCR. The base sequence of the primer of GPx used at this time is as follows.
<프라이머 서열>Primer Sequence
GPx 정방향 프라이머; 5'-tacattgtttgagaagtgcg-3'GPx forward primer; 5'-tacattgtttgagaagtgcg-3 '
GPx 역방향 프라이머; 5'-gacagcagggtttctatgtc -3'GPx reverse primer; 5'-gacagcagggtttctatgtc -3 '
RT-PCR을 시행 후 GPx 의 mRNA의 발현 정도(IU/g protein)를 분석한 결과, 셀레늄과 관련된 대표적인 항산화 효소로 알려진 GPx의 mRNA 발현량이 생리식염수 처리 군에 비해 광천수 처리군에서 현저하게 증가되어 있음을 알 수 있었다(도 4 참조). After RT-PCR analysis, the expression level of mRNA of GPx (IU / g protein) was analyzed. As a result, the expression of GPx, a representative antioxidant enzyme related to selenium, was significantly increased in mineral water treatment group compared to normal saline treatment group. It can be seen that (see Figure 4).
<실시예 4><Example 4>
만성 알코올 중독 마우스 모델을 대상으로 한 본 발명에 따른 광천수의 숙취 해소 활성 분석Hangover Relief Activity Analysis of Mineral Waters according to the Present Invention in a Model of Chronic Alcoholic Mouse
<4-1> 간 및 췌장 무게 측정<4-1> Liver and Pancreas Weight Measurement
만성 알코올 중독 마우스 모델을 제조하기 위해 하기 표 2에 기재된 바와 같은 실험 동물군을 준비하였다. 또한, 만성 알코올 중독 마우스 모델 제조를 위해 만성적으로 지방간을 유도할 수 있는 방법 중 하나인 Lieber-Decarli ethanol diet를 사용하였고, Lieber-Decarli 에탄올 식이는 35% fat, 11% carbohydrate, 18% protein, 36% ethanol이 포함되어 있으며 대조군을 위한 먹이로는 Lieber-Decarli 대조군 식이로 35% fat, 47% carbohydrate, 18% protein이 포함되어 있는 식단을 사용하였다. Experimental animal groups as described in Table 2 were prepared to prepare chronic alcoholism mouse models. In addition, we used Lieber-Decarli ethanol diet, one of the methods to induce chronic fatty liver, to prepare chronic alcoholic mouse model. Lieber-Decarli ethanol diet was 35% fat, 11% carbohydrate, 18% protein, 36 A diet containing% ethanol and 35% fat, 47% carbohydrate, and 18% protein was used as a Lieber-Decarli control diet.
표 2 만성 알코올 중독 마우스 동물군
오전9-오후8 오후8-오전9
A 정상식이+식수 Lieber-Decarli 대조군 식이(50ml/day)
B 정상식이+식수 Lieber-Decarli 에탄올 섭취 식이(50ml/day)
C 정상식이+광천수 10%
D 정상식이+광천수 30%
TABLE 2 Chronic Alcoholism Mouse Fauna
9 am-8 pm 8 pm-9am
A Normal Diet + Drinking Water Lieber-Decarli Control Diet (50ml / day)
B Normal Diet + Drinking Water Lieber-Decarli Ethanol Intake Diet (50ml / day)
C Normal diet + mineral water 10%
D Normal diet + mineral water 30%
상기 4가지 군을 대상으로 한 섭취 스케줄은 먼저 생후 11주부터 13주까지 대조군(A)을 제외한 B,C,D군은 에탄올(3g/kg)을 섭취하게 한 후, 14주~17주까지는 상기 표 2와 같은 식단을 제공하였고, 18주째에 각 마우스들을 희생시켜 이후 실험들을 진행하였다. The intake schedule for the four groups was first to 11 to 13 weeks of age, except for the control group (A) B, C, D group ethanol (3g / kg) intake, and 14 to 17 weeks The diet shown in Table 2 was provided, and each mouse was sacrificed at 18 weeks, and then experiments were performed.
희생된 각 마우스들로부터 간과 췌장의 무게를 측정하였는데, 그 결과, 에탄올만을 처리한 군에 비해(B) 광천수를 처리한 군(C 및 D)에서 간과 췌장의 무게가 모두 감소하는 것으로 나타났으며, 무게의 감소 정도는 섭취한 광천수의 농도에 비례하는 것으로 나타났다(도 5, 6 참조)Liver and pancreas weights were measured from each of the sacrificed mice. As a result, the liver and pancreas weights were decreased in the mineral water treated groups (C and D) compared to the ethanol-only group (B). , The weight loss was proportional to the concentration of mineral water ingested (see FIGS. 5 and 6).
<4-2> AST ,ALT, ALP 의 측정<4-2> Measurement of AST, ALT and ALP
본 발명의 광천수가 에탄올 섭취로 인한 간기능 저하에 치료 효과가 있는지 알아보기 위해, 간기능 검사의 일종인 AST , ALT 및 ALP 지표를 대상으로 분석하였는데, 상기 실시예 <4-1>에서 사용한 동물 모델을 대상으로 수행하였다. In order to determine whether the mineral water of the present invention has a therapeutic effect on liver function deterioration due to ethanol intake, the analysis was performed on AST, ALT and ALP indicators, which are a type of liver function test, and the animals used in Example <4-1>. The model was performed on the subject.
AST 및 ALT는 레이트만(Reitman)등의 방법(Reitman, S., et al., A colormetric method for the determination of serum giutamic oxaloactic and glitamic pyruvic transaminase, Am. J. Clin. Pathol., 28, pp.56-63, 1957)에 준하여 조제된 kit(아산제약)를 사용하여 분석하였고, ALT(100ml 당 DL-alanine 1,780mg 및 a-ketoglutamic acid 29.2mg 함유)및 AST(100ml 당 L-aspartic acid 2,660mg 및 a-ketoglutamic acid 29.2mg 함유)는 기질액 1.0ml를 37℃에서 5분간 미리 배양(preincubation) 시킨 후 준비한 각각의 혈청 0.2ml를 넣어 37℃에서 각각 ALT는 30분, AST는 60분간 반응 시킨 후 정색시액(2,4-dinitrophenylhydrazine, 19.8mg/100ml 함유) 1.0ml를 첨가하고 0.4N-NaOH 용액 1.0ml를 가하여 혼합한 후 10분간 실온에서 방치하고 파장 505nm에서 흡광도(Shimadzu UV-1201, Japan)를 측정하였다.AST and ALT are described in Reitman et al., Reitman, S., et al., A colormetric method for the determination of serum giutamic oxaloactic and glitamic pyruvic transaminase, Am. J. Clin. Pathol., 28, pp. 56-63, 1957) was used to analyze the kit (Asan Pharmaceutical), ALT (containing 1780mg of DL-alanine and 29.2mg of a-ketoglutamic acid per 100ml) and AST (2,660mg of L-aspartic acid per 100ml). And a-ketoglutamic acid containing 29.2 mg) were preincubated with 1.0 ml of substrate solution at 37 ° C. for 5 minutes, and then 0.2 ml of each prepared serum was reacted at 37 ° C. for 30 minutes for ALT and 60 minutes for AST. After adding 1.0 ml of a coloring solution (containing 2,4-dinitrophenylhydrazine, 19.8 mg / 100 ml), and adding 1.0 ml of 0.4 N-NaOH solution, mixed and left at room temperature for 10 minutes and absorbance at wavelength 505 nm (Shimadzu UV-1201, Japan). ) Was measured.
그 결과, AST의 활성은 정상군에 비해 에탄올을 섭취한 군의 경우 거의 2배 정도(95.9±44.4U/L) AST의 활성이 증가한 것으로 나타난 반면, 본 발명의 광천수를 처리한 군은 에탄올 섭취 군에 비해 AST의 활성이 감소하는 것으로 나타났고, 감소 정도는 광천수 처리 농도에 비례하는 것으로 나타났다(10%광천수 처리군:94±41.2U/L, 30% 광천수 처리군: 70.3±14U/L)(도 7 참조).As a result, the activity of AST was found to be increased almost twice as much (95.9 ± 44.4U / L) AST activity in the group ethanol intake compared to the normal group, whereas the group treated with mineral water of the present invention is ethanol intake AST activity was decreased compared to the group, and the decrease was proportional to the mineral water treatment concentration (10% mineral water treatment group: 94 ± 41.2U / L, 30% mineral water treatment group: 70.3 ± 14U / L) (See FIG. 7).
또한, ALT 및 AST의 활성 측정 결과도 AST의 결과와 동일하게 에탄올을 섭취한 군이 정상군에 비해 활성도가 현저하게 증가한 것으로 나타났고, 반면 광천수를 처리한 군에서는 활성도가 감소하는 것으로 나타났으며, 감소 정도는 광천수의 처리 농도에 비례하는 것으로 나타났다(도 8 및 도 9 참조).In addition, the results of the measurement of activity of ALT and AST also showed that the ethanol-ingested group showed a significant increase in activity compared to the normal group, whereas the activity of mineral water decreased in the group treated with mineral water. The degree of reduction was found to be proportional to the treatment concentration of the mineral water (see FIGS. 8 and 9).
<4-3> 총 콜레스테롤 및 트리글리세라이드 함량 측정<4-3> Determination of Total Cholesterol and Triglyceride Content
상기 실시예 <4-1>에서 사용한 동물 모델로부터 혈액을 각각 채취하여 총 콜레트레롤 및 중성지방 수치를 측정하였다.Blood was collected from the animal model used in Example <4-1>, and total cholesterol and triglyceride levels were measured.
총 콜레스테롤은 cholesterol assay kit(Daiichi Sankyo Company, Tokyo, Japan)를 사용하였고 중성지방은 triglyceride assay kit(Wako Pure chemical Industries, Osaka, Japan)를 사용하여 ADVIA 1650 (Bayer Co. Ltd., USA) 자동분석기로 측정하였고 그 결과를 도 10 및 도 11에 나타내었다. Total cholesterol was measured using a cholesterol assay kit (Daiichi Sankyo Company, Tokyo, Japan) and triglycerides were analyzed using an ADVIA 1650 (Bayer Co. Ltd., USA) automated analyzer using a triglyceride assay kit (Wako Pure chemical Industries, Osaka, Japan). And the results are shown in FIGS. 10 and 11.
그 결과, 혈중 총 콜레스테롤의 수치는 에탄올군이 60±10.8mg/dL로 나타났으며, 광천수 처리군은 에탄올 처리군에 비해 감소한 수치를 보였고, 감소 정도는 광천수의 처리 농도에 비례하는 것으로 나타났으며(도 10 참조) 중성지방인 트리글리세라이드도 총 콜레스테롤의 결과와 비슷하게 에탄올을 섭취시킨 군보다 10% 및 30% 광천수를 섭취시킨 군에서 트리글리세라이드 수치가 감소된 것으로 나타났다(도 11 참조).As a result, the serum total cholesterol level was 60 ± 10.8mg / dL in the ethanol group, and the mineral water treatment group was decreased compared to the ethanol treatment group, and the decrease was proportional to the treatment concentration of the mineral water. Triglycerides, triglycerides (see FIG. 10), also showed a decrease in triglyceride levels in the groups ingested 10% and 30% mineral water than in the ethanol group (similar to the results of total cholesterol) (see FIG. 11).
<4-4> 본 발명의 광천수 투여에 따른 간손상 보호 효과<4-4> Protective effect of liver damage according to the mineral water administration of the present invention
본 발명의 광천수가 알코올로 인한 간손상을 보호하는 효과가 있는지를 확인하기 위해 상기 실시예 <4-1>에서 사용한 마우스 모델을 대상으로 간 절제를 하고 10% 인산으로 완충된 포름알데히드 용액으로 간 조직을 고정시킨 후, 10% EDTA에서 8시간 동안 탈회하고 파라핀에 포매하였다. 조직 절편(7um)을 준비하여 헤마톡실린 및 에오신으로 염색하고, 현미경으로 관찰하였다.To determine whether the mineral water of the present invention has an effect of protecting liver damage caused by alcohol, liver resection was performed on the mouse model used in Example <4-1>, and the liver was treated with formaldehyde solution buffered with 10% phosphoric acid. After fixing the tissue, demineralized in 10% EDTA for 8 hours and embedded in paraffin. Tissue sections (7 um) were prepared and stained with hematoxylin and eosin and observed under a microscope.
그 결과, 도 12에서와 같이, 대조군인 정상군에 비해 에탄올을 섭취시킨 군은 간 조직의 파괴 정도가 심한 것으로 관찰되었으며 지방방울(lipid droplet)도 증가한 것으로 나타났다. As a result, as shown in Figure 12, compared to the normal group as a control group ethanol intake was observed that the degree of destruction of liver tissue was severe and the lipid droplets (lipid droplet) also increased.
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시 예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다. So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

Claims (7)

  1. 대한민국 강원도 강릉시 옥계면 금진3리 92-1번지 해안단구 지역 온천지의 지하 1100m 지점에서 취수한 해양성 광천수를 유효성분으로 포함하는 숙취 해소용 조성물.Hangover treatment composition comprising marine mineral water taken from the 1100m underground of the hot spring area, 92-1, Geumjin 3-ri, Okgye-myeon, Gangneung-si, Gangwon-do, Korea.
  2. 제1항에 있어서,The method of claim 1,
    상기 광천수는 미네랄 성분으로서, 칼륨 200~230mg/L, 나트륨 7000~9500mg/L, 칼슘 1400~1700mg/L, 마그네슘 900~1100mg/L, 아연 3~9mg/L, 스트론튬 25~35mg/L, 셀레늄 200~500ug/L, 바나듐 65~75ug/L, 게르마늄 0.5~1.5ug/L, 망간 10~40ug/L, 코발트 1~3ug/L, 티탄 600~950ug/L, 구리 3~9ug/L, 리튬 0.02~0.09mg/L, 염소이온 16000~19500mg/L, 불소이온 1.5~3.0mg/L, 브롬이온 40~56mg/L, 황산이온 3000~4500mg/L, 보론 0.8~1.2mg/L 및 이산화규소 0.5~15mg/L를 함유하는 것을 특징으로 하는 숙취 해소용 조성물.The mineral water is a mineral component, potassium 200 ~ 230mg / L, sodium 7000 ~ 9500mg / L, calcium 1400 ~ 1700mg / L, magnesium 900 ~ 1100mg / L, zinc 3 ~ 9mg / L, strontium 25 ~ 35mg / L, selenium 200 ~ 500ug / L, Vanadium 65 ~ 75ug / L, Germanium 0.5 ~ 1.5ug / L, Manganese 10 ~ 40ug / L, Cobalt 1 ~ 3ug / L, Titanium 600 ~ 950ug / L, Copper 3 ~ 9ug / L, Lithium 0.02 ~ 0.09mg / L, chlorine ion 16000 ~ 19500mg / L, fluorine ion 1.5 ~ 3.0mg / L, bromine ion 40 ~ 56mg / L, sulfate ion 3000 ~ 4500mg / L, boron 0.8 ~ 1.2mg / L and silicon dioxide Hangover elimination composition characterized by containing 0.5 ~ 15mg / L.
  3. 제1항에 있어서,The method of claim 1,
    상기 광천수는 탈염처리 및 증발 농축한 농축물이고, 상기 농축물은 조성물 총 중량을 기준으로 0.5~50%(v/v)로 함유하는 것을 특징으로 하는 숙취 해소용 조성물.The mineral water is a desalted and evaporated concentrate, the concentrate is 0.5 to 50% (v / v) based on the total weight of the composition, hangover relief composition.
  4. 제1항에 있어서,The method of claim 1,
    상기 광천수는 혈중 알코올의 농도를 감소시키고, 알코올에 의한 산화적스트레스를 감소시키는 효과를 갖는 것을 특징으로 하는 숙취 해소용 조성물.The mineral water to reduce the concentration of alcohol in the blood, hangover relief composition characterized in that it has the effect of reducing the oxidative stress caused by alcohol.
  5. 제1항에 있어서,The method of claim 1,
    상기 광천수는 알코올 분해효소인 간알콜탈수소화효소(ADH) 및 알데히드탈수소화효소(ALDH)의 발현을 증가시키고, 항산화 효소인 GPx(Glutathione peroxidase)의 발현 증가를 통해 숙취 해소 활성을 갖는 것을 특징으로 하는 숙취 해소용 조성물.The mineral water increases the expression of hepatic alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which are alcohol degrading enzymes, and has a hangover-relieving activity through increased expression of the antioxidant enzyme GPx (Glutathione peroxidase). Hangover cure composition.
  6. 제1항에 있어서,The method of claim 1,
    상기 광천수는 알코올로 인해 증가된 체내 총 콜레스테롤 함량 및 중성지방(triglyceride)의 함량을 감소시키는 활성을 갖는 것을 특징으로 하는 숙취 해소용 조성물. The mineral water is hangover relief composition characterized in that it has an activity to reduce the total cholesterol content and triglyceride content in the body increased due to alcohol.
  7. 제1항 내지 제6항 중 어느 한 항의 조성물을 유효성분으로 함유하는 숙취 해소를 위한 건강기능성식품. Health functional food for hangover relief containing the composition of any one of claims 1 to 6.
PCT/KR2012/010198 2011-12-22 2012-11-29 Composition for clearing hangovers containing mineral water WO2013094890A1 (en)

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