WO2013091661A3 - Etiquette d'affinité protéique résistante à la protéolyse - Google Patents

Etiquette d'affinité protéique résistante à la protéolyse Download PDF

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Publication number
WO2013091661A3
WO2013091661A3 PCT/DK2012/050505 DK2012050505W WO2013091661A3 WO 2013091661 A3 WO2013091661 A3 WO 2013091661A3 DK 2012050505 W DK2012050505 W DK 2012050505W WO 2013091661 A3 WO2013091661 A3 WO 2013091661A3
Authority
WO
WIPO (PCT)
Prior art keywords
affinity tag
protein affinity
resistant protein
proteolytic resistant
proline
Prior art date
Application number
PCT/DK2012/050505
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English (en)
Other versions
WO2013091661A2 (fr
Inventor
Giuseppe Dionisio
Henrik Brinch-Pedersen
Anne Lind ROSENKILDE
Original Assignee
Aarhus Universitet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aarhus Universitet filed Critical Aarhus Universitet
Publication of WO2013091661A2 publication Critical patent/WO2013091661A2/fr
Publication of WO2013091661A3 publication Critical patent/WO2013091661A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne une étiquette protéique qui comprend un motif acide aminé Proline-X-Proline (PXP), où X est choisi parmi l'un quelconque des acides aminés. De plus, l'invention concerne des polypeptides de fusion et une cellule hôte comprenant l'étiquette protéique, ainsi que des acides nucléiques codant pour ceux-ci. De plus, l'invention concerne des procédés de production d'une protéine recombinante par la mise en culture de la cellule hôte, l'expression d'un polypeptide de fusion et l'isolement du polypeptide de fusion à partir de la culture.
PCT/DK2012/050505 2011-12-23 2012-12-21 Etiquette d'affinité protéique résistante à la protéolyse WO2013091661A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201170757 2011-12-23
DKPA201170757 2011-12-23

Publications (2)

Publication Number Publication Date
WO2013091661A2 WO2013091661A2 (fr) 2013-06-27
WO2013091661A3 true WO2013091661A3 (fr) 2013-08-15

Family

ID=47561008

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2012/050505 WO2013091661A2 (fr) 2011-12-23 2012-12-21 Etiquette d'affinité protéique résistante à la protéolyse

Country Status (1)

Country Link
WO (1) WO2013091661A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107267475A (zh) * 2017-08-11 2017-10-20 浙江福斯特新材料研究院有限公司 一种金属螯合亲和色谱纯化硫氧还蛋白的方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3009880A1 (fr) * 2015-12-28 2017-07-06 Idemitsu Kosan Co., Ltd. Marqueur peptidique et proteine marquee comprenant celui-ci
US11446398B2 (en) 2016-04-11 2022-09-20 Obsidian Therapeutics, Inc. Regulated biocircuit systems
CN106188234B (zh) * 2016-07-27 2019-05-17 中国石油大学(华东) 一种与金属离子具有更强结合力的蛋白纯化标签及其应用
US20190352365A1 (en) * 2017-01-18 2019-11-21 Savior Lifetec Corporation Expression construct and method for producing proteins of interest
EP3806888B1 (fr) 2018-06-12 2024-01-31 Obsidian Therapeutics, Inc. Constructions régulatrices dérivées de pde5 et procédés d'utilisation en immunothérapie
US20210386788A1 (en) 2018-10-24 2021-12-16 Obsidian Therapeutics, Inc. Er tunable protein regulation
KR20210149251A (ko) 2019-03-08 2021-12-08 옵시디안 테라퓨틱스, 인크. 조율가능한 조절을 위한 인간 탄산 무수화효소 2 조성물 및 방법
US20220267398A1 (en) 2019-06-12 2022-08-25 Obsidian Therapeutics, Inc. Ca2 compositions and methods for tunable regulation
BR112021025022A2 (pt) 2019-06-12 2022-02-22 Obsidian Therapeutics Inc Composições de ca2 e métodos para regulação ajustáveis
US20220348937A1 (en) 2019-09-06 2022-11-03 Obsidian Therapeutics, Inc. Compositions and methods for dhfr tunable protein regulation
CN111690039B (zh) * 2020-07-02 2022-04-05 南开大学 识别6xHis标签蛋白的自组装多肽探针、制备方法及应用

Citations (7)

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Publication number Priority date Publication date Assignee Title
WO1997007132A1 (fr) * 1995-08-15 1997-02-27 Commonwealth Scientific And Industrial Research Organisation Systeme de marquage par epitopes
WO2001031019A2 (fr) * 1999-10-29 2001-05-03 Chiron Spa Peptides antigeniques de neisseria
WO2003040170A2 (fr) * 2001-11-09 2003-05-15 Pfizer Products Inc. Anticorps anti-cd40
WO2004001424A1 (fr) * 2002-06-24 2003-12-31 University Court Of The University Of Aberdeen Materiaux et procedes d'induction de tolerance immunitaire
US20060099710A1 (en) * 2004-11-10 2006-05-11 Donnelly Mark I Vector for improved in vivo production of proteins
US20060286047A1 (en) * 2005-06-21 2006-12-21 Lowe David J Methods for determining the sequence of a peptide motif having affinity for a substrate
US20090215106A1 (en) * 2005-09-05 2009-08-27 Iris Pribilla Method for detecting intracellular interaction between biomolecules

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997007132A1 (fr) * 1995-08-15 1997-02-27 Commonwealth Scientific And Industrial Research Organisation Systeme de marquage par epitopes
WO2001031019A2 (fr) * 1999-10-29 2001-05-03 Chiron Spa Peptides antigeniques de neisseria
WO2003040170A2 (fr) * 2001-11-09 2003-05-15 Pfizer Products Inc. Anticorps anti-cd40
WO2004001424A1 (fr) * 2002-06-24 2003-12-31 University Court Of The University Of Aberdeen Materiaux et procedes d'induction de tolerance immunitaire
US20060099710A1 (en) * 2004-11-10 2006-05-11 Donnelly Mark I Vector for improved in vivo production of proteins
US20060286047A1 (en) * 2005-06-21 2006-12-21 Lowe David J Methods for determining the sequence of a peptide motif having affinity for a substrate
US20090215106A1 (en) * 2005-09-05 2009-08-27 Iris Pribilla Method for detecting intracellular interaction between biomolecules

Non-Patent Citations (6)

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Title
ANONYMOUS: "BaculoDirect N-term BAculorvirus Expression system", PRODUCT LITTERATURE BROCHURE, 1 January 2003 (2003-01-01), USA, pages 1 - 2, XP055059254, Retrieved from the Internet <URL:http://tools.invitrogen.com/content/sfs/brochures/BaculoDirect_N_terminal.pdf> [retrieved on 20130411] *
ANONYMOUS: "pcDNA 3.1/V5-His A,B, and C User Manual", CATALOG NO V810-20, 9 November 2010 (2010-11-09), pages 1 - 19, XP055059252, Retrieved from the Internet <URL:http://tools.invitrogen.com/content/sfs/manuals/pcdna3_1v5his_man.pdf> [retrieved on 20130411] *
D. SCHNEIDER ET AL: "2,3,7,8-Tetrachlorodibenzo-p-dioxin-Mediated Impairment of B Cell Differentiation Involves Dysregulation of Paired Box 5 (Pax5) Isoform, Pax5a", JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, vol. 326, no. 2, 15 May 2008 (2008-05-15), pages 463 - 474, XP055059133, ISSN: 0022-3565, DOI: 10.1124/jpet.108.139857 *
DATABASE EMBL [Online] 24 August 2004 (2004-08-24), "Pleurotus djamor beta-1,3 exoglucanase precursor-like protein", XP002695233, retrieved from EBI Database accession no. AAS46752 *
JINGFA LIU ET AL: "Protein-folding simulations of the hydrophobic-hydrophilic model by combining pull moves with energy landscape paving.", PHYSICAL REVIEW E, vol. 84, no. 3, 30 September 2011 (2011-09-30), pages 031934 - 031934, XP055059028, ISSN: 1539-3755 *
LI YIFENG: "Commonly used tag combinations for tandem affinity purification", BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, vol. 55, no. Part 2, 15 February 2010 (2010-02-15), ACADEMIC PRESS, US, pages 73 - 83, XP002672627, ISSN: 0885-4513, [retrieved on 20101223], DOI: 10.1042/BA20090273 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107267475A (zh) * 2017-08-11 2017-10-20 浙江福斯特新材料研究院有限公司 一种金属螯合亲和色谱纯化硫氧还蛋白的方法

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