WO2013090799A1 - Silicon-containing fumaric acid esters - Google Patents

Silicon-containing fumaric acid esters Download PDF

Info

Publication number
WO2013090799A1
WO2013090799A1 PCT/US2012/069872 US2012069872W WO2013090799A1 WO 2013090799 A1 WO2013090799 A1 WO 2013090799A1 US 2012069872 W US2012069872 W US 2012069872W WO 2013090799 A1 WO2013090799 A1 WO 2013090799A1
Authority
WO
WIPO (PCT)
Prior art keywords
compound
alkyl
compounds
methyl
aryl
Prior art date
Application number
PCT/US2012/069872
Other languages
French (fr)
Inventor
Jianhua Chao
Original Assignee
Biogen Idec Ma Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Idec Ma Inc. filed Critical Biogen Idec Ma Inc.
Priority to US14/363,546 priority Critical patent/US9421273B2/en
Priority to EP12856877.1A priority patent/EP2791181A4/en
Publication of WO2013090799A1 publication Critical patent/WO2013090799A1/en
Priority to HK15103505.5A priority patent/HK1202885A1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07FACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F7/00Compounds containing elements of Groups 4 or 14 of the Periodic Table
    • C07F7/02Silicon compounds
    • C07F7/08Compounds having one or more C—Si linkages
    • C07F7/18Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
    • C07F7/1804Compounds having Si-O-C linkages
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07FACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F7/00Compounds containing elements of Groups 4 or 14 of the Periodic Table
    • C07F7/02Silicon compounds
    • C07F7/08Compounds having one or more C—Si linkages
    • C07F7/0834Compounds having one or more O-Si linkage
    • C07F7/0836Compounds with one or more Si-OH or Si-O-metal linkage
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07FACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F7/00Compounds containing elements of Groups 4 or 14 of the Periodic Table
    • C07F7/02Silicon compounds
    • C07F7/08Compounds having one or more C—Si linkages
    • C07F7/18Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
    • C07F7/1896Compounds having one or more Si-O-acyl linkages

Definitions

  • the present invention provides a compound of Formula III: or a pharmaceutically acceptable salt thereof, wherein:
  • R 2 is C1-C10 alkyl.
  • the present invention provides a method of treating a subject having a condition characterized by at least one symptom chosen from neurodegeneration and neuroinflammation comprising administering to a subject a compound of Formula I:
  • the present invention provides a method of treating a subject having a condition characterized by at least one symptom chosen from neurodegeneration and neuroinflammation comprising administering to a subject a compound of Formula II: ( ⁇ )
  • the present invention provides a method of treating a subject having a condition characterized by at least one symptom chosen from neurodegeneration and neuroinflammation comprising administering to a subject a compound of Formula III: or a pharmaceutically acceptable salt thereof, wherein:
  • R 1 is C1-C24 alkyl or C 6 -Cio aryl
  • each of m, n, and r, independently, is 0-4;
  • R 3 , R 4 , and R 5 are [0026] Another group of compounds of Formula I include compounds wherein R is optionally substituted C1-C24 alkyl. Another group of compounds of Formula I include compounds wherein R 1 is optionally substituted Ci-C 6 alkyl. Another group of compounds of Formula I include compounds wherein R 1 is optionally substituted methyl, ethyl, or isopropyl. Another group of compounds of Formula I include compounds wherein R 1 is optionally substituted C5-C50 aryl. Another group of compounds of Formula I include compounds wherein R 1 is optionally substituted C5-C10 aryl. Another group of compounds of Formula I include compounds wherein R is C1-C10 alkyl.
  • compounds of Formula II include compounds wherein each of R and R is, independently, optionally substituted C5-C10 aryl.
  • R 1 is C1-C24 alkyl or C5-C50 aryl
  • fumaric acid ester 1 can be prepared by reacting alcohol (R ! -OH) under the coupling conditions of hydroxybenzotriazole (HOBT), l-ethyl-3-(3- dimethylaminopropyl)carbodiimide (EDCl), and diisopropyl amine (DIPEA) as shown in Scheme 3.
  • HOBT hydroxybenzotriazole
  • EDCl l-ethyl-3-(3- dimethylaminopropyl)carbodiimide
  • DIPEA diisopropyl amine
  • the compounds of this invention of Formula III may be prepared by the exemplary reaction in Scheme 7.
  • expression of the gene is measured by determining the expression level of an mR A for that gene.
  • expression of the gene is measured by determining the expression level of a protein product encoded by the gene.
  • the protein product is measured in cerebrospinal fluid or blood (e.g., plasma) of the subject.
  • expression level is measured at least at one time point selected from prior to initiation of treatment, during treatment, and after treatment.
  • the amount of active ingredient that can be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated, and the particular mode of administration. It should be understood, however, that a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated. The amount of active ingredient can also depend upon the therapeutic or prophylactic agent, if any, with which the ingredient is co-administered.
  • the compounds and pharmaceutical compositions of the invention can be administered in an amount ranging from about 1 mg/kg to about 50 mg/kg (e.g., from about 2.5 mg/kg to about 20 mg/kg or from about 2.5 mg/kg to about 15 mg/kg).
  • the amount of the compounds and pharmaceutical compositions of the invention administered will also vary, as recognized by those skilled in the art, dependent on route of administration, excipient usage, and the possibility of co-usage with other therapeutic treatments including use of other therapeutic agents.
  • Suitable excipients are, in particular, fillers such as saccharides, for example lactose or sucrose, mannitol or sorbitol, cellulose preparations and/or calcium phosphates, for example tricalcium phosphate or calcium hydrogen phosphate, as well as binders such as starch paste, using, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, tragacanth, methyl cellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, and/or polyvinyl pyrrolidone.
  • fillers such as saccharides, for example lactose or sucrose, mannitol or sorbitol, cellulose preparations and/or calcium phosphates, for example tricalcium phosphate or calcium hydrogen phosphate, as well as binders such as starch paste, using, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, tragacanth, methyl cellulose,
  • concentrated saccharide solutions may be used, which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, polyethylene glycol and/or titanium dioxide, lacquer solutions and suitable organic solvents or solvent mixtures.
  • suitable cellulose preparations such as acetylcellulose phthalate or hydroxypropymethyl-cellulose phthalate, are used.
  • Dye stuffs or pigments may be added to the tablets or dragee coatings, for example, for identification or in order to characterize combinations of active compound doses.
  • the pharmaceutical preparations comprise a capsule containing the compound or pharmaceutical composition of the present invention in the form of an enteric-coated microtablet.
  • the coating of the microtablet may be composed of different layers.
  • the first layer may be a methyacrylic acid - methyl methacrylate copolymer/isopropyl solution which isolates the tablet cores from potential hydrolysis from the next applied water suspensions.
  • the enteric coating of the tablet may then be conferred by an aqueous methacrylic acid - ethyl acrylate copolymer suspension.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The present invention is directed to silicon-containing fumaric acid esters of the Formulae I-IV. The silicon- containing fumaric acid esters of Formulae I-IV are useful in transplantation medicine and for the treatment of autoimmune diseases and autoimmune-related diseases. (I), (II), (III) & (IV)

Description

SILICON-CONTAINING FUMARIC ACID ESTERS
BRIEF SUMMARY OF THE INVENTION
[0001] The methods provided are exemplary and are not intended to limit the scope of the claimed embodiments.
[0002] In one embodiment, the present invention provides a compound of Formula I:
Figure imgf000002_0001
or a pharmaceutically acceptable salt thereof, wherein:
R is Ci-Cio alkyl, C6-Cio aryl, hydroxyl, -O-Ci-Cio alkyl, or -O-C6-C10 aryl;
each of R3, R4, and R5, independently, is C1-C10 alkyl, C6-Cio aryl, hydroxyl,
-O-Ci-Cio alkyl, -O-C6-C10 aryl, or
Figure imgf000002_0002
wherein R is C1-C24 alkyl or C6-Cio aryl; each of which can be optionally substituted; and
each of m, n, and r, independently, is 0-4;
provided that at least one of R3, R4, and R5 is
Figure imgf000002_0003
[0003] In another embodiment, the present invention provides a compound of Formula II:
Figure imgf000002_0004
or a pharmaceutically acceptable salt thereof, wherein:
R1 is C1-C24 alkyl or C6-Cio aryl; and
2 3
each of, independently, R and R , is C1-C10 alkyl or C6-Cio aryl. [0004] In one embodiment, the present invention provides a compound of Formula II, wherein R1 is methyl, ethyl, or isopropyl. In another embodiment, the present invention provides a compound of Formula II wherein each of R 2 and R 3 , independently, is methyl, ethyl, or isopropyl.
[0005] In another embodiment, the present invention provides a compound of Formula III:
Figure imgf000003_0001
or a pharmaceutically acceptable salt thereof, wherein:
R1 is C1-C24 alkyl or C6-Cio aryl;
2 3 5
each of R , R , and R , independently, is hydroxyl, C1-C10 alkyl, C6-Cio aryl, -O-Ci-Cio alkyl, or -O-C6-C10 aryl; and
n is 1 or 2.
[0006] In one embodiment, the present invention provides a compound of Formula III, wherein each of R 2 , R 3 , and R 5 , independently, is hydroxyl, methyl, or ethyl. In another embodiment, the present invention provides a compound of Formula III, wherein n is 1.
[0007] In another embodiment, the present invention provides a compound of Formula IV:
Figure imgf000003_0002
or a pharmaceutically acceptable salt thereof, wherein:
R1 is C1-C24 alkyl or C6-Cio aryl; and
R2 is C1-C10 alkyl.
[0008] In one embodiment, the present invention provides a compound of Formula IV, wherein each R1, independently, is is methyl, ethyl, or isopropyl. In another embodiment, the present invention provides a compound of Formula IV, wherein R is methyl, ethyl, or isopropyl. [0009] In another embodiment, the present invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and one or more compounds of Formula I, Formula II, Formula III, Formula IV, or mixtures thereof.
[0010] In another embodiment, the present invention provides a method of treating a subject having a condition characterized by at least one symptom chosen from neurodegeneration and neuroinflammation comprising administering to a subject a compound of Formula I:
Figure imgf000004_0001
or a pharmaceutically acceptable salt thereof, wherein:
R is Ci-Cio alkyl, C6-Cio aryl, hydroxyl, -O-Ci-Cio alkyl, or -O-C6-C10 aryl;
each of R3, R4, and R5, independently, is C1-C10 alkyl, C6-Cio aryl, hydroxyl, -O- C1-C10 alkyl, -O-C6-C10 aryl, or
Figure imgf000004_0002
wherein R is C1-C24 alkyl or C6-Cio aryl; each of which can be optionally substituted; and
each of m, n, and r, independently, is 0-4;
provided that at least one of R3, R4, and R5 is
Figure imgf000004_0003
[0011] In another embodiment, the present invention provides a method of treating a subject having a condition characterized by at least one symptom chosen from neurodegeneration and neuroinflammation comprising administering to a subject a compound of Formula II: (Π)
Figure imgf000005_0001
or a pharmaceutically acceptable salt thereof, wherein:
R1 is C1-C24 alkyl or C6-Cio aryl; and
2 3
each of, independently, R and R , is C1-C10 alkyl or C6-Cio aryl.
[0012] In another embodiment, the present invention provides a method of treating a subject having a condition characterized by at least one symptom chosen from neurodegeneration and neuroinflammation comprising administering to a subject a compound of Formula III:
Figure imgf000005_0002
or a pharmaceutically acceptable salt thereof, wherein:
R1 is C1-C24 alkyl or C6-Cio aryl;
2 3 5
each of R , R , and R , independently, is hydroxyl, C1-C10 alkyl, C6-Cio aryl, -O-Ci-Cio alkyl, or -O-C6-C10 aryl; and
n is 1 or 2.
[0013] In another embodiment, the present invention provides a method of treating a subject having a condition characterized by at least one symptom chosen from neurodegeneration and neuroinflammation comprising administering to a subject a compound of Formula IV:
Figure imgf000005_0003
or a pharmaceutically acceptable salt thereof, wherein:
R1 is C1-C24 alkyl or C6-Cio aryl; and
R2 is Ci-Ci0 alkyl.
[0014] In one embodiment, the present invention provides a method of treating a subject having a condition characterized by at least one symptom chosen from neurodegeneration and neuroinflammation wherein the condition is multiple sclerosis, Huntington's disease, Amyotrophic lateral sclerosis, Alzheimer's disease, or Parkinson's disease by administering a compound of Formula I, Formula II, Formula III, or Formula IV.
[0015] In one embodiment, the present invention provides a compound of Formula I selected from the group consisting of: (dimethylsilanediyl)dimethyl difumarate, methyl ((trimethoxysilyl)methyl) fumarate, methyl ((trihydroxysilyl)methyl) fumarate, and trimethyl (methylsilanetriyl) trifumarate.
BRIEF DESCRIPTION OF THE DRAWINGS
[0016] FIGURE la provides the luciferase induction fold change after 24 hours of stimulation with the compounds dimethyl fumarate (DMF), monomethyl fumarate (MMF), (E)-0,0'-(dimethylsilanediyl)dimethyl difumarate (Compound 11), and methyl ((trimethoxysilyl)methyl) fumarate (Compound 12) based on compound concentration in the DLD-1/ARE-E2 cell reporter cell line.
[0017] FIGURE lb provides the luciferase induction fold change after 48 hours of stimulation with the compounds dimethyl fumarate (DMF), monomethyl fumarate (MMF), (E)-0,0'-(dimethylsilanediyl)dimethyl difumarate (Compound 11), and methyl ((trimethoxysilyl)methyl) fumarate (Compound 12) based on compound concentration in the DLD-1/ARE-E2 cell reporter cell line.
[0018] FIGURE 2a provides the luciferase induction fold change after 24 hours of stimulation with the compounds dimethyl fumarate (DMF), monomethyl fumarate (MMF), (E)-0,0'-(dimethylsilanediyl)dimethyl difumarate (Compound 11), and methyl ((trimethoxysilyl)methyl) fumarate (Compound 12) based on compound concentration in the MCF7/ARE-D3 cell reporter cell line.
[0019] FIGURE 2b provides the luciferase induction fold change after 48 hours of stimulation with the compounds dimethyl fumarate (DMF), monomethyl fumarate (MMF), (E)-0,0'-(dimethylsilanediyl)dimethyl difumarate (Compound 11), and methyl ((trimethoxysilyl)methyl) fumarate (Compound 12) based on compound concentration in the MCF7/ARE-D3 cell reporter cell line.
[0020] FIGURE 3 provides the luciferase induction fold change after 24 hours of stimulation with the compounds dimethyl fumarate (DMF), monomethyl fumarate (MMF), (E)-0,0'-(dimethylsilanediyl)dimethyl difumarate (Compound 11), and methyl ((trimethoxysilyl)methyl) fumarate (Compound 12) based on compound concentration in the MCF7/ARE-D3 cell reporter cell line.
DETAILED DESCRIPTION OF THE INVENTION
[0021] Dimethyl fumarate (DMF) has been shown to have significant therapeutic effects on patients having multiple sclerosis. See, e.g., Kappos et al, Lancet 572: 1463-1472 (2008) and Nature Drug Reviews 10:404 (2011). Preclinical studies indicate that the therapeutic effects of DMF appear to be mediated through activation of the nuclear factor- E2-related factor 2 (Nrf2) transcriptional pathway, which is a major pathway to combat oxidative stress associated with several different neurological diseases (see, e.g., WO 2008/097596 and Calkins et al., Antioxid. Redox Signal 77^:497-508 (2009)). DMF is rapidly metabolized into monomethyl fumarate (MMF) upon administration. The present invention discloses silicon-containing compounds, which like DMF, can metabolize into MMF upon administration.
[0022] The silicon atom, although similar to carbon, has a larger covalent radius (¾ = 117 pm) than carbon (rc = 77 pm) and therefore forms longer and slightly more flexible bonds which result in subtle changes in molecular size and shape of the silicon analogs. Furthermore, silicon is more electropositive which causes a slight change in bond polarization. These differences affect how silicon analogs interact with enzymes and proteins which could give rise to different pharmacological effects and metabolism. For example, sila-haloperidol has shown lack of a major pyridinium metabolite, wherein this metabolite was directly linked to neurotoxic side effects observed by the carbon analog haloperidol. Johansson, T., et al, Drug Metabolism & Disposition 35:73-83 (2010).
[0023] Additionally, the fact that silicon is more electropositive would make it possible to differentiate between the two ester groups in the disclosed fumarate molecules. This would allow for regioselective hydrolysis of the silicon-containing ester group to occur and would unmask the active drug ingredient. [0024] Silicon analogs are in general more lipophilic. A small increase in lipophilicity will markedly increase the absorption, volume of distribution, and tissue penetration of a drug, leading to a lesser degree of hepatic metabolism and enhance the plasma half-life of a drug. In addition, increasing lipophilicity may also improve the permeability through the blood brain barrier, enhancing central nervous system activity. See Pooni, P.K., et al., Mini-Reviews in Med. Chem. (5: 1 169-1 177 (2006). The silyl group confers lipophilicity that can enhance the penetration of the compounds across the gut wall, cell membranes, and blood brain barrier, thus improving therapeutic properties including bioavailability, metabolism, and pharmacokinetics.
[0025] The present invention provides silicon-containing fumaric acid esters represented by Formula I:
Figure imgf000008_0001
or a pharmaceutically acceptable salt thereof, wherein:
R is Ci-Cio alkyl, C5-Ci5 aryl, hydroxyl, -O-Ci-Cio alkyl, or -O-C5-C15 aryl;
each of R3, R4, and R5, independently, is Ci-Cio alkyl, C5-C15 aryl, hydroxyl, -O- C1-C10 alkyl, -O-C5-C15 aryl, or
Figure imgf000008_0002
wherein R is C1-C24 alkyl or C5-C50 aryl; each of which can be optionally substituted; and
each of m, n, and r, independently, is 0-4;
provided that at least one of R3, R4, and R5 is
Figure imgf000008_0003
[0026] Another group of compounds of Formula I include compounds wherein R is optionally substituted C1-C24 alkyl. Another group of compounds of Formula I include compounds wherein R1 is optionally substituted Ci-C6 alkyl. Another group of compounds of Formula I include compounds wherein R1 is optionally substituted methyl, ethyl, or isopropyl. Another group of compounds of Formula I include compounds wherein R1 is optionally substituted C5-C50 aryl. Another group of compounds of Formula I include compounds wherein R1 is optionally substituted C5-C10 aryl. Another group of compounds of Formula I include compounds wherein R is C1-C10 alkyl. Another group of compounds of Formula I include compounds wherein R is optionally substituted Ci-C6 alkyl. Another group of compounds of Formula I include compounds wherein R is optionally substituted methyl, ethyl, or isopropyl. Another group of compounds of Formula I include compounds wherein R is optionally substituted C5-C15 aryl. Another group of compounds of Formula I include compounds wherein R is optionally substituted C5-C10 aryl.
[0027] In another embodiment, the invention provides compounds represented by Formula
II:
Figure imgf000009_0001
or a pharmaceutically acceptable salt thereof, wherein:
2
each of, independently, R and R , is C1-C10 alkyl or C5-C15 aryl.
2
R" and RJ can be the same or different, can be optionally substituted, and independently can be selected from the group consisting of C1-C10 alkyl or C5-C15 aryl.
[0028] In another embodiment, compounds of Formula II include compounds wherein R1 is optionally substituted C1-C24 alkyl. Another group of compounds of Formula II include compounds wherein R1 is optionally substituted Ci-C6 alkyl. Another group of compounds of Formula II include compounds wherein R1 is optionally substituted methyl, ethyl, or isopropyl. Another group of compounds of Formula II include compounds wherein R1 is optionally substituted C5-C50 aryl. Another group of compounds of Formula II include compounds wherein R1 is optionally substituted C5-C10 aryl. alkyl. Another 2 3 group of compounds of Formula II include compounds wherein each of R and R is, independently, optionally substituted Ci-Cio alkyl. Another group of compounds of
Formula II include compounds wherein each of R 2 and R 3 is, independently, optionally substituted Ci-C6 alkyl. Another group of compounds of Formula II include compounds wherein each of R 2 and R 3 is, independently, optionally substituted methyl, ethyl, or isopropyl. Another group of compounds of Formula II include compounds wherein each of R 2 and R 3 is, independently, optionally substituted C5-Ci5 aryl. Another group of
2 3 compounds of Formula II include compounds wherein each of R and R is, independently, optionally substituted C5-C10 aryl.
[0029] In another embodiment, the invention provides compounds represented by Formula
III:
Figure imgf000010_0001
or a pharmaceutically acceptable salt thereof, wherein:
R1 is C1-C24 alkyl or C5-C50 aryl;
2 3 5
each of R , R , and R , independently, is hydroxyl, C1-C10 alkyl, C5-C15 aryl, -O-Ci-Cio alkyl, or -O-C5-C15 aryl; and
n is 1 or 2.
[0030] In another embodiment, compounds of Formula III include compounds wherein R1 is optionally substituted C1-C24 alkyl. Another group of compounds of Formula III include compounds wherein R1 is optionally substituted Ci-C6 alkyl. Another group of compounds of Formula III include compounds wherein R1 is optionally substituted methyl, ethyl, or isopropyl. Another group of compounds of Formula III include compounds wherein R1 is optionally substituted C5-C50 aryl. Another group of compounds of Formula III include compounds wherein R1 is optionally substituted C5-C10 aryl.
2 3
Another group of compounds of Formula III include compounds wherein each of R , R , and R5 is, independently, hydroxyl. Another group of compounds of Formula III include
2 3 5
compounds wherein each of R , R , and R is, independently, optionally substituted Ci- C10 alkyl. Another group of compounds of Formula III include compounds wherein each
2 3 5
of R , R , and R is, independently, optionally substituted Ci-C6 alkyl. Another group of
2 3 5 compounds of Formula III include compounds wherein each of R , R , and R is, independently, optionally substituted methyl, ethyl, or isopropyl. Another group of
2 3 5 compounds of Formula III include compounds wherein each of R , R , and R is, independently, optionally substituted C5-Ci5 aryl. Another group of compounds of
Formula III include compounds wherein each of R 2 , R 3 , and R 5 is, independently, optionally substituted C5-C10 aryl.
[0031] In another embodiment, the invention provides compounds represented by Formula
IV:
Figure imgf000011_0001
or a pharmaceutically acceptable salt thereof, wherein:
R1 is C1-C24 alkyl or C5-C50 aryl; and
R2 is C1-C10 alkyl.
[0032] In another embodiment, compounds of Formula IV include compounds wherein R1 is optionally substituted C1-C24 alkyl. Another group of compounds of Formula IV include compounds wherein R1 is optionally substituted Ci-C6 alkyl. Another group of compounds of Formula IV include compounds wherein R1 is optionally substituted methyl, ethyl, or isopropyl. Another group of compounds of Formula IV include compounds wherein R1 is optionally substituted C5-C50 aryl. Another group of compounds of Formula IV include compounds wherein R1 is optionally substituted C5-C10 aryl. Another group of compounds of Formula IV include compounds wherein R is optionally substituted Ci-C6 alkyl. Another group of compounds of Formula IV include compounds wherein R is optionally substituted methyl, ethyl, or isopropyl.
[0033] The term "alkyl" as employed herein by itself or as part of another group refers to both straight and branched chain radicals of up to 24 carbons. Alkyl groups include straight- chained and branched C1-C24 alkyl groups, e.g., C1-C10 alkyl groups. C1-C10 alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, neopentyl, hexyl, isohexyl, 3-methylpentyl, 2,2-dimethylbutyl, 2,3- dimethylbutyl, heptyl, 1 -methylhexyl, 2-ethylhexyl, 1 ,4-dimethylpentyl, octyl, nonyl, and decyl. Unless otherwise indicated, all alkyl groups described herein include both unsubstituted and substituted alkyl groups. Further, each alkyl group can include its deuterated counterparts.
[0034] The term "aryl" as employed herein by itself or as part of another group refers to monocyclic, bicyclic, or tricyclic aromatic groups containing from 5 to 50 carbons in the ring portion. Aryl groups include C5-15 aryl, e.g., phenyl, p-tolyl, 4-methoxyphenyl, 4- (tert-butoxy)phenyl, 3-methyl-4-methoxyphenyl, 4-fluorophenyl, 4-chlorophenyl, 3- nitrophenyl, 3-aminophenyl, 3-acetamidophenyl, 4-acetamidophenyl, 2-methyl-3- acetamidophenyl, 2-methyl-3-aminophenyl, 3-methyl-4-aminophenyl, 2-amino-3- methylphenyl, 2,4-dimethyl-3-aminophenyl, 4-hydroxyphenyl, 3-methyl-4- hydroxyphenyl, 1-naphthyl, 3-amino-naphthyl, 2-methyl-3-amino-naphthyl, 6-amino-2- naphthyl, 4,6-dimethoxy-2-naphthyl, indanyl, biphenyl, phenanthryl, anthryl, and acenaphthyl. Unless otherwise indicated, all aryl groups described herein include both unsubstituted and substituted aryl groups.
[0035] Optional substituents on the alkyl group include one or more substituents independently selected from halogen, hydroxyl, carboxyl, amino, nitro, or cyano.
[0036] Optional substituents on the aryl group include one or more substituents independently selected from alkyl, alkoxy, halogen, hydroxyl, or amino.
[0037] Halogen groups include fluorine, chlorine, bromine, and iodine.
[0038] Some of the compounds of the present invention may exist as stereoisomers including optical isomers. The invention includes all stereoisomers and both the racemic mixtures of such stereoisomers as well as the individual enantiomers that may be separated according to methods that are well-known to those of ordinary skill in the art.
[0039] The compounds of the invention may be prepared using methods known to those skilled in the art, or the novel methods of this invention.
[0040] Specifically, the compounds of this invention of Formula I may be prepared by the exemplary reaction in Scheme 1.
Scheme 1
Figure imgf000012_0001
wherein R , R , and R are each defined above for Formula II.
[0041] Reaction of fumaric acid ester 1 with silane diacetate intermediate 2 in a refluxing organic solvent such as diethyl ether, toluene, or hexane to give the desired siloxane 3.
[0042] Some of the fumaric acid esters 1 are commercially available. Fumaric acid ester 1 can also be prepared, for example, using synthetic methods known by one of ordinary skill in the art. For example, fumaric acid can be converted by reacting alcohol (R!-OH) with a catalytic amount of p-toluene sulfonic acid at room temperature for a few hours to overnight as shown in Scheme 2.
Scheme 2
Figure imgf000013_0001
wherein R is defined above for Formula I.
[0043] Alternatively, fumaric acid ester 1 can be prepared by reacting alcohol (R!-OH) under the coupling conditions of hydroxybenzotriazole (HOBT), l-ethyl-3-(3- dimethylaminopropyl)carbodiimide (EDCl), and diisopropyl amine (DIPEA) as shown in Scheme 3.
Scheme 3
Figure imgf000013_0002
wherein R is defined above for Formula I.
[0044] Some of the silanes that can be used in the present invention are commercially available. Commercially available silyl halides include trimethylsilyl chloride, dichloro- methylphenylsilane, dimethyldichlorosilane, methyltrichlorosilane, (4- aminobutyl)diethoxymethylsilane, trichloro(chloromethyl)silane, trichloro(dichlorophenyl)silane, trichloroethylsilane, trichlorophenylsilane, and trimethylchlorosilane. Commercial sources for silyl halides include Sigma Aldrich and Acros Organics. [0045] Silanes used in the present invention can be prepared, for example, using synthetic methods known by one of ordinary skill in the art. For example, trichlorosilane may be prepared by the exemplary reaction in Scheme 4.
Scheme 4
R CI
R ^ + HSiCI ^ ¾r
^ 3 C Ι
[0046] The silylation of styrene derivatives catalyzed by palladium is described in Zhang,
F. and Fan, Q.-H., Organic & Biomolecular Chemistry 7 1Q- 1 (2009) and in Bell,
J.R., et al, Tetrahedron 65:9368-9372 (2009).
[0047] Diacetate intermediate 2 may be prepared by treatment of dichlorosubstituted silicon compound 4 with sodium acetate in diethyl ether under reflux as shown in Scheme
5.
Scheme 5
CI R2 NaOAc Et20 AcO R2
- sr
C 'R3 reflux AcQf 'R3 wherein R 2 and R 3 are each defined above for Formula I.
[0048] Specifically, the compounds of this invention of Formula III may be prepared by the exemplary reaction in Scheme 6.
Scheme 6
Figure imgf000014_0001
wherein R 1 , R2 , R 3 , and R 5 are as defined above for Formula III.
[0049] Fumaric acid ester 1 can be converted to the sodium salt 5 using, for example, sodium methoxide in methanol at room temperature. Removal of the solvent would afford sodium salt 5. Treatment of the sodium salt 5 with silane 6 in an organic solvent such as dimethylformamide under reflux would generate ester 7. The synthesis of structurally related (trimethoxysilyl) -methyl esters is described in Voronkov, M.G., et al., Zhurnal Obshchei Khimii 52:2052-2055 (1982).
[0050] Alternatively, the compounds of this invention of Formula III may be prepared by the exemplary reaction in Scheme 7.
Scheme 7
base, heat
Figure imgf000015_0001
(Ill) wherein R1, R4, R5, R6, and n are as defined above for Formula III.
[0051] Treatment of the sodium salt 5 with silane 6 in an organic solvent such as dimethylformamide under heating with or without an acid scavenger would generate ester 7.
Scheme 8
Figure imgf000015_0002
wherein R1, R4, R5, R6, and n are as defined above for Formula III.
[0052] Reaction of fumaric acid ester 1 with tri-substituted silane alcohol 8 in methylene chloride with mild base such as triethyl amine and 4-N,N-dimethyl amino pyridine (DMAP) at room temperature generates fumarate 7. See Coelho, P.J., et al., Eur. J. Org. Chem. 3039-3046 (2000). [0053] Specifically, the compounds of this invention of Formula IV can be prepared by emplary reaction in Scheme 9.
Scheme 9
Figure imgf000016_0001
10
1 2
wherein R and R are as defined above for Formula IV.
[0054] Reaction of fumaric acid 1 with trichlorosilane 9 in a refluxing organic solvent such as hexane or toluene using a catalytic amount of a base such as triethylamine generates the trifumarate silane 10. The reaction of acetic and methacrylic acids with 1- silyladamantanes is described in Fedotov, N.S., et al., Zhurnal Obshchei Khimii 52: 1837- 1842 (1982).
[0055] The present invention includes a therapeutic method comprising administering to an animal an effective amount of a compound of Formulae I-IV, or a pharmaceutically acceptable salt of said compound of Formulae I-IV, wherein said therapeutic method is useful for treating a subject having a condition or at risk of having a condition characterized by at least one symptom chosen from neurodegeneration and neuroinflammation. A condition characterized by at least one of neurodegeneration and neuroinflammation is a condition in which either or both of those processes leads to a failure of the subjects nervous system to function normally. The loss of normal function may be located in either or both of the central nervous system or it can affect the immune system in general (e.g., the brain or spinal cord) and the peripheral nervous system or it can affect the immune system in general (e.g., in circulation and in tissues). Examples of such conditions include, but are not limited to, Adrenal Leukodystrophy (ALD), Alcoholism, Alexander's disease, Alper's disease, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS or Lou Gehrig's Disease), Ataxia telangiectasia, Batten disease (also known as Spielmeyer-Vogt-Sjorgren-Batten disease), Bovine spongiform encephalopathy (BSE), Canavan disease, Cerebral palsy, Cockayne syndrome, Corticobasal degeneration, Creutzfeldt- Jakob disease, Familial Fatal Insomnia, Frontotemporal lobar degeneration, Huntington's disease, HIV-associated dementia, Kennedy's disease, Krabbe's disease, Lewy body dementia, Neuroborreliosis, Machado-Joseph disease (Spinocerebellar ataxia type 3), Multiple System Atrophy, Multiple sclerosis, Narcolepsy, Niemann Pick disease, Parkinson's disease, Pelizaeus-Merzbacher Disease, Pick's disease, Primay lateral sclerosis, Prion diseases, Progressive Supranuclear Palsy, Refsum's disease, Sandhoff disease, Schilder's disease, Subacute combined degeneration of spinal cord secondary to Pernicious Anaemia, Spielmeyer-Vogt-Sjogren-Batten disease (also known as Batten disease), Spinocerebellar ataxia, Spinal muscular atrophy, Steele-Richardson-Olszewski disease, Tabes dorsalis, Toxic encephalopathy, LHON (Leber's Hereditary optic neuropathy), MELAS (Mitochondrial Encephalomyopathy; Lactic Acidosis; Stroke), MERRF (Myoclonic Epilepsy; Ragged Red Fibers), PEO (Progressive External Opthalmoplegia), Leigh's Syndrome, MNGIE (Myopathy and external ophthalmopolegia; Neuropathy; Gastro-Intestinal; Encephalopathy), Kearns-Sayre Syndrome (KSS), NARP, Hereditary Spastic Paraparesis, Mitochondrial myopathy, and Friedreich Ataxia.
[0056] In one embodiment, the condition is multiple sclerosis. In another embodiment, the condition is relapsing-remitting multiple sclerosis. In another embodiment, the condition is secondary-progressive multiple sclerosis. In another embodiment, the condition is primary-progressive multiple sclerosis. In another embodiment, the condition is progressive-relapsing multiple sclerosis.
[0057] In one embodiment, the condition is Amyotrophic lateral sclerosis (ALS).
[0058] In one embodiment, the condition is Huntington's disease.
[0059] In one embodiment, the condition is Parkinson's disease.
[0060] In one embodiment, the condition is Alzheimer's disease.
[0061] In some embodiments, administration of at least one compound or pharmaceutically acceptable salt thereof, as described herein, to a patient gives rise to "neuroprotection," or said another way, the effect of administering the compound to the patient is neuroprotection. Neuroprotection comprises at least one of maintenance, salvage, recovery, and regeneration of the nervous system, its cells, structure, and function following injury or damage. In some embodiments, neuroprotection comprises at least one of primary neuroprotection and secondary neuroprotection. "Primary neuroprotection" is protection comprising direct modulation of the structure and/or function of neural cells residing within the CNS (at least one cell type selected from neurons, oligodendrocytes, astrocytes, and microglia). "Secondary neuroprotection" is protection comprising modulation of the structure or function of at least one cell type that typically resides outside the CNS (e.g. immune cells). In secondary neuroprotection, the at least one compound or pharmaceutically acceptable salt thereof acts directly or indirectly on the at least one cell type that typically resides outside the CNS to modulate the structure and/or function of the at least one cell type. The at least one cell type then modulates, directly or otherwise, the structure and/or function of neural cells residing within the CNS (at least one cell type selected from neurons, oligodendrocytes, astrocytes, and microglia). In some embodiments, neuroprotection comprises a lessening of the severity or rate of neurodegeneration or neuroinflammation in a subject. "Maintenance" of the nervous system, its cells, structure, and function comprises embodiments in which the at least one compound or pharmaceutically acceptable salt thereof is administered to a subject prior to development of at least one sign or symptom of a disease or condition disclosed herein and reduces the eventual severity of the disease or condition and/or reduces the rate of onset of the disease and/or condition.
[0062] In some embodiments, the condition to be treated is characterized by increased expression of pro-inflammatory genes, such as in neural cells of the subject. In the case of a subject experiencing astrogliosis, for example, expression of at least one proinflammatory gene selected from Ccl20, Ccl3, Ccl4, Cxcll, CxcllO, Cxcl2, Cxcl3, Cxcl6, ILla, Illb, TNF, Ifit3, Nfkbia, Nfkbiz, Tnfaip2, and Zc3hl2a is increased in the subject. In some embodiments, administration of at least a compound, or a pharmaceutically acceptable salt thereof, as described herein, to the subject, results in suppression of expression of at least one gene selected from Ccl20, Ccl3, Ccl4, Cxcll, CxcllO, Cxcl2, Cxcl3, Cxcl6, ILla, Illb, TNF, Ifit3, Nfkbia, Nfkbiz, Tnfaip2, and Zc3hl2a.
[0063] Certain examples of neuroprotective genes are also disclosed herein, namely
Gsta2, Gsta3, Gclc, Ggtl, Txnrdl, Srxnl, Sqstml, and NQOl . In some embodiments, administration of at least one compound or pharmaceutically acceptable salt thereof, as described herein, to the subject, results in an up-regulation of at least one gene selected from Gsta2, Gsta3, Gclc, Ggtl, Txnrdl, Srxnl, Sqstml, and NQOl .
[0064] The term "therapeutically effective amount" refers to that amount of a compound or pharmaceutically acceptable salt thereof which results in prevention or delay of onset or amelioration of at least one symptom of a condition characterized by neurodegeneration or neuroinflammation in a subject, or an attainment of a desired biological outcome, such as reduced astrogliosis. [0065] In some embodiments, the expression level of at least one gene selected from
Ccl20, Ccl3, Ccl4, Cxcll, CxcllO, Cxcl2, Cxcl3, Cxcl6, ILla, Illb, TNF, Ifit3, Nfkbia, Nfkbiz, Tnfaip2, Zc3hl2a, Gsta2, Gsta3, Gclc, Ggtl, Txnrdl, Srxnl, Sqstml, and NQOl is measured in a subject. In some embodiments, expression of the gene is measured by determining the expression level of an mR A for that gene. In some embodiments, expression of the gene is measured by determining the expression level of a protein product encoded by the gene. In some embodiments, the protein product is measured in cerebrospinal fluid or blood (e.g., plasma) of the subject. In some embodiments, expression level is measured at least at one time point selected from prior to initiation of treatment, during treatment, and after treatment.
[0066] The ability of compounds of the present invention to activate the Nrf-2 antioxidant response pathway can be tested according to Example 5 below. The compounds can also be evaluated in other cell based assays or animal model(s) of neuroinflammatory and neurodegenerative diseases. For example, compounds of the present invention can be evaluated for their ability to treat ALS in a cell-based motor neuron survival assay as described in Haidet-Phillips et al, Nat Biotechnol. 2P(¾):824-828 (201 1). For animal model studies, compounds of the present invention can be investigated in models for Alzheimer's disease (see Dumont et al, J. Neurochem. 109(2):502-512 (2009)), Parkinson's disease (see Jakel et al., Brain Res. 1144: 192-201 (2007)), ALS (see Wegorzewska et al., Proc. Natl. Acad. Sci. US A. 706^: 18809-14 (2009); Wong et al, Neuron 14(6): 1105-1116 (1995); and Vargas et al, J. Neurosci. 25^: 13574-13581 (2008)), Huntington's disease (see Stack et al, Free Radic. Biol. Med. 49(2): \47-\58 (2010)), and MS (the Experimental Autoimmune Encephalomyelitis (EAE) model; see, e.g., Tuohy et al, J. Immunol. 747: 1126-1130 (1988), Sobel et al, J. Immunol. 752:2393-2401 (1984), and Traugott, Cell Immunol. 77M 14-129 (1989)).
[0067] The term "treating" refers to administering a therapy in an amount, manner, and/or mode effective to prevent or delay onset of or amelioration of at least one symptom of a condition characterized by neurodegeneration or neuroinflammation in a subject, to either a statistically significant degree or to a degree detectable to one skilled in the art. An effective amount, manner, or mode can vary depending on the subject and may be tailored to the subject. For neurological disorders referred herein, the treatment offered by the method of this invention aims at improving the conditions (or lessening the detrimental effects) of the disorders and not necessarily at completely eliminating or curing the disorders.
[0068] The compounds and pharmaceutical compositions of the present invention may be administered by any means that achieve their intended purpose. For example, administration may be by parenteral, subcutaneous, intravenous, intramuscular, intraperitoneal, transdermal, buccal, intrathecal, intracranial, intranasal, or topical routes. Alternatively, or concurrently, administration may be by the oral route. The dosage administered will be dependent upon the age, health, and weight of the recipient, kind of concurrent treatment, if any, frequency of treatment, and the nature of the effect desired.
[0069] The amount of active ingredient that can be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated, and the particular mode of administration. It should be understood, however, that a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated. The amount of active ingredient can also depend upon the therapeutic or prophylactic agent, if any, with which the ingredient is co-administered.
[0070] In some embodiments, the compounds and pharmaceutical compositions of the invention can be administered in an amount ranging from about 1 mg/kg to about 50 mg/kg (e.g., from about 2.5 mg/kg to about 20 mg/kg or from about 2.5 mg/kg to about 15 mg/kg). The amount of the compounds and pharmaceutical compositions of the invention administered will also vary, as recognized by those skilled in the art, dependent on route of administration, excipient usage, and the possibility of co-usage with other therapeutic treatments including use of other therapeutic agents.
[0071] For example, the compounds and pharmaceutical compositions of the invention can be administered to a subject, for example orally, in an amount of from about 0.1 g to about 1 g per day, or for example, in an amount of from about 100 mg to about 800 mg per day.
[0072] The amount of compounds and pharmaceutical compositions of the invention may be administered in separate administrations of 2, 3, 4, 5 or 6 equal doses per day.
[0073] In addition to administering the compound as a raw chemical, the compounds of the invention may be administered as part of a pharmaceutical preparation containing suitable pharmaceutically acceptable carriers comprising excipients and auxiliaries which facilitate processing of the compounds into preparations which may be used pharmaceutically. For example, the preparations, particularly those preparations which may be administered orally and which may be used for the preferred type of administration, such as tablets, dragees, and capsules, and also preparations which may be administered rectally, such as suppositories, as well as suitable solutions for administration by injection or orally, contain from about 0.01 to 99 percent, preferably from about 0.25 to 75 percent of active compound(s), together with the excipient.
[0074] Also included within the scope of the present invention are the non-toxic pharmaceutically acceptable salts of the compounds of the present invention. Acid addition salts are formed by mixing a solution of the compound of Formulae I-IV with a solution of a pharmaceutically acceptable non-toxic acid such as hydrochloride, hydrobromide, hydroiodide, nitrate, sulfate, bisulfate, phosphate, acid phosphate, isonicotinate, acetate, lactate, salicylate, citrate, tartrate, pantothenate, bitartrate, ascorbate, succinate, maleate, gentisinate, gluconate, glucaronate, saccharate, formate, benzoate, glutamate, methanesulfonate, ethanesulfonate, benzenesulfonate, p-toluenesulfonate, and pamoate. Acceptable base salts include aluminum, calcium, lithium, magnesium, potassium, sodium, zinc, and diethanolamine salts.
[0075] The pharmaceutical compositions of the invention may be administered to any animal which may experience the beneficial effects of the compounds of the invention. Foremost among such animals are mammals, e.g., humans and veterinary animals, although the invention is not intended to be so limited.
[0076] The pharmaceutical preparations of the present invention are manufactured in a manner which is itself known, for example, by means of conventional mixing, granulating, dragee-making, dissolving, or lyophilizing processes. Thus, pharmaceutical preparations for oral use may be obtained by combining the active compounds with solid excipients, optionally grinding the resulting mixture and processing the mixture of granules, after adding suitable auxiliaries, if desired or necessary, to obtain tablets or dragee cores.
[0077] Suitable excipients are, in particular, fillers such as saccharides, for example lactose or sucrose, mannitol or sorbitol, cellulose preparations and/or calcium phosphates, for example tricalcium phosphate or calcium hydrogen phosphate, as well as binders such as starch paste, using, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, tragacanth, methyl cellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, and/or polyvinyl pyrrolidone. If desired, disintegrating agents may be added such as the above-mentioned starches and also carboxymethyl-starch, cross- linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof, such as sodium alginate. Auxiliaries are, above all, flow-regulating agents and lubricants, for example, silica, talc, stearic acid or salts thereof, such as magnesium stearate or calcium stearate, and/or polyethylene glycol. Dragee cores are provided with suitable coatings which, if desired, are resistant to gastric juices. For this purpose, concentrated saccharide solutions may be used, which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, polyethylene glycol and/or titanium dioxide, lacquer solutions and suitable organic solvents or solvent mixtures. In order to produce coatings resistant to gastric juices, solutions of suitable cellulose preparations such as acetylcellulose phthalate or hydroxypropymethyl-cellulose phthalate, are used. Dye stuffs or pigments may be added to the tablets or dragee coatings, for example, for identification or in order to characterize combinations of active compound doses.
[0078] In one embodiment, the pharmaceutical preparations comprise a capsule containing the compound or pharmaceutical composition of the present invention in the form of an enteric-coated microtablet. The coating of the microtablet may be composed of different layers. The first layer may be a methyacrylic acid - methyl methacrylate copolymer/isopropyl solution which isolates the tablet cores from potential hydrolysis from the next applied water suspensions. The enteric coating of the tablet may then be conferred by an aqueous methacrylic acid - ethyl acrylate copolymer suspension.
[0100] Other pharmaceutical preparations which may be used orally include push- fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer such as glycerol or sorbitol. The push-fit capsules may contain the active compounds in the form of granules which may be mixed with fillers such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds are preferably dissolved or suspended in suitable liquids, such as fatty oils, or liquid paraffin. In addition, stabilizers may be added.
[0101] Possible pharmaceutical preparations which may be used rectally include, for example, suppositories, which consist of a combination of one or more of the active compounds with a suppository base. Suitable suppository bases are, for example, natural or synthetic triglycerides, or paraffin hydrocarbons. In addition, it is also possible to use gelatin rectal capsules which consist of a combination of the active compounds with a base. Possible base materials include, for example, liquid triglycerides, polyethylene glycols, or paraffin hydrocarbons.
[0102] Suitable formulations for parenteral administration include aqueous solutions of the active compounds in water-soluble form, for example, water-soluble salts and alkaline solutions. In addition, suspensions of the active compounds as appropriate oily injection suspensions may be administered. Suitable lipophilic solvents or vehicles include fatty oils, for example, sesame oil, or synthetic fatty acid esters, for example, ethyl oleate or triglycerides or polyethylene glycol-400 (the compounds are soluble in PEG-400), or cremophor, or cyclodextrins. Aqueous injection suspensions may contain substances which increase the viscosity of the suspension and include, for example, sodium carboxymethyl cellulose, sorbitol, and/or dextran. Optionally, the suspension may also contain stabilizers.
[0103] In accordance with one aspect of the present invention, compounds of the invention are employed in topical and parenteral formulations and are used for the treatment of psoriasis. The topical compositions of this invention are formulated preferably as oils, creams, lotions, ointments and the like by choice of appropriate carriers. Suitable carriers include vegetable or mineral oils, white petrolatum (white soft paraffin), branched chain fats or oils, animal fats and high molecular weight alcohol (greater than C12). The preferred carriers are those in which the active ingredient is soluble. Emulsifiers, stabilizers, humectants and antioxidants may also be included as well as agents imparting color or fragrance, if desired. Additionally, transdermal penetration enhancers may be employed in these topical formulations.
[0104] Creams are preferably formulated from a mixture of mineral oil, self-emulsifying beeswax and water in which mixture the active ingredient, dissolved in a small amount of oil such as almond oil, is admixed. A typical example of such a cream is one which includes about 40 parts water, about 20 parts beeswax, about 40 parts mineral oil and about 1 part almond oil.
[0105] Ointments may be formulated by mixing a solution of the active ingredient in a vegetable oil such as almond oil with warm soft paraffin and allowing the mixture to cool. A typical example of such an ointment is one which includes about 30% almond oil and about 70% white soft paraffin by weight. [0106] Having now generally described this invention, the same will be understood by reference to the following examples which are provided herein for purposes of illustration only and are not intended to be limiting unless otherwise specified.
EXAMPLES
Example 1
Synthesis of (E)-0,0'-(dimethylsilanediyl)dimethyl difumarate (Compound 11)
Figure imgf000024_0001
[0107] Step 1 : Preparation of dimethylsilanediyl diacetate 11B
O
p Et20, reflux, 2 h 0-^~-~
^Sf + NaOAc ^Si'
11A 1 1 B b
[0108] To a slurry of sodium acetate (8.2 g, 100 mmol, 2.0 equiv.) in anhydrous diethyl ether (40 mL) was slowly added a solution of dimethyldichloro silane 11A (6.45 g, 50 mmol, 1.0 equiv.) in anhydrous diethyl ether (10 mL). After addition was completed, the mixture was heated at reflux for 2 hours, and then filtered under N2. The filtrate was concentrated under vacuum at 40 °C to give diacetate 1 IB as a colorless oil (6.1 g, 70%). 1H NMR (400 MHz, CDC13) δ ppm: 2.08 (s, 6H), 0.48 (s, 6H).
[0109] Step 2: Preparation of (^-O,O '- dimethylsilanediyl)dimethyl difumarate 11
Figure imgf000025_0001
11 B 11
[0110] A mixture of 11B (2.0 mL, 12 mmol, 1.5 equiv.) and 11C (1.04 g, 8.0 mmol, 1.0 equiv.) in a sealed tube was heated at 170 °C with stirring under microwave condition for 1 hour. After cooling to 50 °C, the mixture was transferred to a round bottom flask and the excess silica reactant 11B was removed under vacuum at 100 °C to afford compound 11 as brown oil (1.47 g, 60%). 1H NMR (400 MHz, CDC13) δ ppm: 6.82-6.80 (m, 4H), 3.79 (s, 6H), 0.57 (s, 6H).
EXAMPLE 2
Synthesis of methyl ((trimethoxysilyl)methyl) fumarate (Compound 12)
Figure imgf000025_0002
[0111] To a stirred solution of monomethyl fumarate (3.5 g, 27 mmol, 1.0 equiv.) in anhydrous THF (35 mL) at room temperature was added sodium hydride (1.08 g, 27 mmol, 1.0 equiv.) in small portions. After addition, the mixture was heated to reflux for 3 hours, and then cooled to room temperature. The solid was collected by filtration and washed twice with diethyl ether, and further dried in vacuo to give 3.8 g of 12B (93%).
[0112] To a suspension of 12B (760 mg, 5.0 mmol, 1.0 equiv.) in dry DMA (5 mL) at 100
°C under nitrogen was added a solution of 12A (1.03 g, 6.0 mmol, 1.2 equiv.) in dry DMA (1 mL) dropwise. The resulting mixture was heated to 160 °C and stirred for 1 hour, and then cooled to room temperature. The solid was filtered, and the filtrate was evaporated under reduced pressure to give the titled compound 12, 513 mg (37%), as a red viscous liquid.
[0113] 1H NMR (400 MHz, CDC13) δ ppm: 6.90-6.86 (m, 2H), 3.97 (s, 2H), 3.82 (s, 3H),
3.62 (s, 9H).
EXAMPLE 3
Synthesis of methyl ((trihydroxysilyl)methyl fumarate (Compound 13)
Figure imgf000026_0001
12 13
[0114] To a solution of 12 (1.0 g, 3.8 mmol, 1.0 equiv., prepared in Example 2) in
MeOH (10 mL) at room temperature was added water (341 mg, 19.0 mmol, 5.0 equiv.) dropwise. After addition, the mixture was stirred at room temperature for 30 minutes, with white solids precipitated out. The solids were collected through filtration, washed with methanol three times, and dried at 60 °C in vacuo, to provide the titled compound 13, 500 mg (59%), as a white solid.
[0115] 1H NMR (400 MHz, DMSO- 6) δ ppm: 6.79-6.74 (m, 2H), 3.91-3.58 (m, 6H),
3.18-3.15 (m, 2H).
EXAMPLE 4
Synthesis of trimethyl (methylsilanetriyl) trifumarate (Compound 14)
Figure imgf000026_0002
[0116] Following the procedure described in Scheme 9, monomethyl fumarate 14A would react with trichloromethane-silane 14B in refluxing toluene or hexanes with a catalytic amount of triethylamine to provide (2 Έ, 2 "E)-trimethyl O,O ',O"-(¾iethylsilanetriyl) trifumarate 14C.
EXAMPLE 5
Nrf2 activation cell-based assay
[0117] Human cancer cell line DLD-1 and breast cancer cell line MCF7 reporter stable cell lines were generated by transfection with a firefly luciferase reporter construct harboring the luciferase cDNA cloned downstream of eight catenated copies of the antioxidant response element (ARE: SEQ ID NO.: l).
[0118] To measure Nrf2 activation in the ARE-luciferase reporter cell lines, the cells were plated in 96-well plates at 20-50k cells/well 24 hours prior to stimulation with the test compounds. The test compounds were prepared in dimethylsulfoxide (DMSO) and diluted with culture media to required concentrations (final DMSO concentrations <0.3%). The reporter cells were harvested 24 hours - 48 hours after addition of the compounds and lysed for detection of luciferase activity. Luciferase activity in the lysates was monitored using the Bright-Glo Luciferase Assay System of Promega and Tecan Genios Pro plate reader.
[0119] Luciferase induction in the compound-treated cells was calculated as fold change over the baseline activity detected in control cultures treated with DMSO-containing media.
Table 1
Figure imgf000027_0001
Figure imgf000027_0002
[0100] Table 1 indicates that Compound 11 and Compound 12 are able to activate ARE- dependent signaling of the luciferase reporter construct. This suggests the compounds are able to activate the Nrf2 signaling cascade and induce expression of genes downstream of the ARE. EXAMPLE 6
In vivo evaluation of Nrf2 activation
[0101] Test compounds were dosed either in suspension of 0.8% HPMC or corn oil via oral gavage to male SD rats (average weight of 250 mg, 6 animals per group, two groups), at a dose of 100 mg/kg equivalent of DMF (dosing volume: 5 ml/kg). After 30 minutes, the first group of animals was sacrificed via C02 asphyxiation. 1.0 mL blood sample via cardiac bleed pipetted into chilled lithium heparin tubes with 10 mg sodium fluoride. Samples were centrifuged within 30 minutes at 4 °C for 15 minutes at 1500G and plasma was transferred to chilled tubes and immediately frozen on dry ice, further kept at -70 °C until shipment for analysis. Brain was removed; sections were weighed and frozen until analysis. Brain and plasma samples were analyzed for monomethylfumaric acid ester (MMF) exposure. After 6 h, the second group of animals was sacrificed via C02 asphyxiation. Brain, spleen, liver and jejunum were removed, flash frozen and placed on dry ice and maintained frozen until analysis. Sections of brain, spleen, liver, and jejunum were submitted for qPCR analysis of relative expression increase of Nrf2 responsive enzymes such as NQO-1, Akrlb8, and Sulfiredoxin-1.
[0102] Having now fully described this invention, it will be understood by those of ordinary skill in the art that the same can be performed within a wide and equivalent range of conditions, formulation and other parameters without affecting the scope of the invention or any embodiment thereof. All patents, patent applications and publications cited herein are fully incorporated by reference herein in their entirety.

Claims

WHAT IS CLAIMED IS:
A compound having Formula I
Figure imgf000029_0001
or a pharmaceutically acceptable salt thereof, wherein:
R is Ci-Cio alkyl, C6-Cio aryl, hydroxyl, -O-Ci-Cio alkyl, or -O-C6-C10 aryl;
each of R3, R4, and R5, independently, is C1-C10 alkyl, C6-Cio aryl, hydroxyl, -O-Ci-Cio alkyl, -O-C6-C10 aryl, or
Figure imgf000029_0002
wherein R is C1-C24 alkyl or C6-Cio aryl; each of which can be optionally substituted; and
each of m, n, and r, independently, is 0-4;
provided that at least one of R3, R4, and R5 is
Figure imgf000029_0003
2. The compound of claim 1 having Formula II:
Figure imgf000029_0004
or a pharmaceutically acceptable salt thereof, wherein:
2 3
each of, independently, R and R , is C1-C10 alkyl or C6-Cio aryl;
2 3
R" and RJ can be the same or different, can be optionally substituted, and independently can be selected from the group consisting of C1-C10 alkyl or C6-Cio aryl. The compound of claim 2, wherein each of R 2 and R 3 , independently, is methyl, ethyl, or isopropyl.
The compound of claim 2, wherein R1 is methyl, ethyl, or isopropyl. The compound of claim 1 having Formula III:
Figure imgf000030_0001
or a pharmaceutically acceptable salt thereof, wherein:
R1 is C1-C24 alkyl or C6-Cio aryl;
2 3 5
each of R , R , and R , independently, is hydroxyl, C1-C10 alkyl, C6-Cio aryl, -O-Ci-Cio alkyl, or -O-C6-C10 aryl; and
n is 1 or 2.
2 3 5
The compound of claim 5, wherein each of R , R , and R , independently, is hydroxyl, methoxy, ethoxy, methyl, or ethyl.
The compound of claim 5, wherein n is 1.
The compound of claim 1 having Formula IV:
Figure imgf000030_0002
or a pharmaceutically acceptable salt thereof, wherein:
R1 is C1-C24 alkyl or C6-Cio aryl; and
R2 is C1-C10 alkyl.
9. The compound of claim 8, wherein R is methyl, ethyl, or isopropyl.
10. The compound of claim 8, wherein each R1, independently, is methyl, ethyl, or isopropyl.
11. The compound of claim 1 , selected from the group consisting of:
(dimethylsilanediyl)dimethyl difumarate;
methyl ((trimethoxysilyl)methyl) fumarate;
methyl ((trihydroxysilyl)methyl) fumarate; and
trimethyl (methylsilanetriyl) trifumarate.
12. A method of treating a subject having a condition characterized by at least one symptom chosen from neurodegeneration and neuroinflammation comprising administering to a subject a compound of claim 1, claim 2, claim 5, claim 8, or claim 1 1.
13. The method of claim 12, wherein the condition characterized by at least symptom chosen from neurodegeneration and neuroinflammation is multiple sclerosis, Huntington's disease, Amytrophic lateral sclerosis (ALS), Alzheimer's disease, and Parkinson's disease.
14. The method of claim 12, wherein the compound is administered in an amount from about 100 mg to about 800 mg per day.
PCT/US2012/069872 2011-12-16 2012-12-14 Silicon-containing fumaric acid esters WO2013090799A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US14/363,546 US9421273B2 (en) 2011-12-16 2012-12-14 Silicon-containing fumaric acid esters
EP12856877.1A EP2791181A4 (en) 2011-12-16 2012-12-14 Silicon-containing fumaric acid esters
HK15103505.5A HK1202885A1 (en) 2011-12-16 2015-04-09 Silicon-containing fumaric acid esters

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161576828P 2011-12-16 2011-12-16
US61/576,828 2011-12-16

Publications (1)

Publication Number Publication Date
WO2013090799A1 true WO2013090799A1 (en) 2013-06-20

Family

ID=48613236

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/069872 WO2013090799A1 (en) 2011-12-16 2012-12-14 Silicon-containing fumaric acid esters

Country Status (4)

Country Link
US (1) US9421273B2 (en)
EP (1) EP2791181A4 (en)
HK (1) HK1202885A1 (en)
WO (1) WO2013090799A1 (en)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8669281B1 (en) 2013-03-14 2014-03-11 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US8980832B2 (en) 2003-09-09 2015-03-17 Biogen Idec International Gmbh Use of fumaric acid derivatives for treating cardiac insufficiency, and asthma
US9326965B2 (en) 2014-02-28 2016-05-03 Banner Life Sciences Llc Controlled release fumarate esters
US9326947B1 (en) 2014-02-28 2016-05-03 Banner Life Sciences Llc Controlled release fumarate esters
WO2016153957A3 (en) * 2015-03-20 2016-11-10 Biogen Ma Inc. Methods and compositions for the intravenous administration of fumarates for the treatment of neurological diseases
US9505776B2 (en) 2013-03-14 2016-11-29 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US9566259B1 (en) 2015-08-31 2017-02-14 Banner Life Sciences Llc Fumarate ester dosage forms
US9604922B2 (en) 2014-02-24 2017-03-28 Alkermes Pharma Ireland Limited Sulfonamide and sulfinamide prodrugs of fumarates and their use in treating various diseases
US10098863B2 (en) 2014-02-28 2018-10-16 Banner Life Sciences Llc Fumarate esters
US10399924B2 (en) 2012-12-21 2019-09-03 Biogen Ma Inc. Deuterium substituted fumarate derivatives
US11903918B2 (en) 2020-01-10 2024-02-20 Banner Life Sciences Llc Fumarate ester dosage forms with enhanced gastrointestinal tolerability

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2334378T3 (en) 2008-08-19 2014-09-30 Xenoport Inc Prodrugs of methyl hydrogen fumarate, pharmaceutical compositions thereof, and methods of use
US10945984B2 (en) 2012-08-22 2021-03-16 Arbor Pharmaceuticals, Llc Methods of administering monomethyl fumarate and prodrugs thereof having reduced side effects
JP2015526477A (en) 2012-08-22 2015-09-10 ゼノポート,インコーポレイティド Oral dosage form of methylhydrogen fumarate and its prodrug
US10179118B2 (en) 2013-03-24 2019-01-15 Arbor Pharmaceuticals, Llc Pharmaceutical compositions of dimethyl fumarate
WO2014197860A1 (en) 2013-06-07 2014-12-11 Xenoport, Inc. Method of making monomethyl fumarate
WO2014205392A1 (en) 2013-06-21 2014-12-24 Xenoport, Inc. Cocrystals of dimethyl fumarate
US9416096B2 (en) 2013-09-06 2016-08-16 Xenoport, Inc. Crystalline forms of (N,N-Diethylcarbamoyl)methyl methyl (2E)but-2-ene-1,4-dioate, methods of synthesis and use
US9999672B2 (en) 2014-03-24 2018-06-19 Xenoport, Inc. Pharmaceutical compositions of fumaric acid esters

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060241240A1 (en) * 2003-03-26 2006-10-26 Marcel Vos Process for the preparation of poly(silyl ester)s, and their uses
US20090011986A1 (en) * 2005-05-18 2009-01-08 Rajendra Kumar Joshi Thiosuccinic Acid Derivatives and the Use Thereof
US20090306015A1 (en) * 2005-12-20 2009-12-10 Silamed, Inc. Pharmaceutical compositions and methods of use of highly lipophilic sulfhydryl compounds
WO2010126605A1 (en) * 2009-04-29 2010-11-04 Biogen Idec Ma Inc. Treatment of neurodegeneration and neuroinflammation

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0812340B2 (en) 1986-04-07 1996-02-07 日本油脂株式会社 contact lens
JP2805794B2 (en) 1988-05-06 1998-09-30 エヌオーケー株式会社 Manufacturing method of gas separation membrane
JP2790021B2 (en) * 1993-09-30 1998-08-27 日本油脂株式会社 Paint composition
ES2916604T1 (en) 2007-02-08 2022-07-04 Biogen Ma Inc Nrf2 Detection Assays and Related Methods and Compositions
DE102007042948A1 (en) 2007-09-10 2009-03-12 Wacker Chemie Ag Moisture-crosslinkable polymers based on α-heteroatom-substituted silanes
DE102008002163A1 (en) 2008-06-02 2009-12-03 Wacker Chemie Ag Binders based on polymers containing alpha-silane groups

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060241240A1 (en) * 2003-03-26 2006-10-26 Marcel Vos Process for the preparation of poly(silyl ester)s, and their uses
US20090011986A1 (en) * 2005-05-18 2009-01-08 Rajendra Kumar Joshi Thiosuccinic Acid Derivatives and the Use Thereof
US20090306015A1 (en) * 2005-12-20 2009-12-10 Silamed, Inc. Pharmaceutical compositions and methods of use of highly lipophilic sulfhydryl compounds
WO2010126605A1 (en) * 2009-04-29 2010-11-04 Biogen Idec Ma Inc. Treatment of neurodegeneration and neuroinflammation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2791181A4 *

Cited By (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8980832B2 (en) 2003-09-09 2015-03-17 Biogen Idec International Gmbh Use of fumaric acid derivatives for treating cardiac insufficiency, and asthma
US10399924B2 (en) 2012-12-21 2019-09-03 Biogen Ma Inc. Deuterium substituted fumarate derivatives
US9505776B2 (en) 2013-03-14 2016-11-29 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US12076306B2 (en) 2013-03-14 2024-09-03 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US11905298B2 (en) 2013-03-14 2024-02-20 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US11679092B2 (en) 2013-03-14 2023-06-20 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US8669281B1 (en) 2013-03-14 2014-03-11 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US11230548B2 (en) 2013-03-14 2022-01-25 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US11083703B2 (en) 2013-03-14 2021-08-10 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US10596140B2 (en) 2013-03-14 2020-03-24 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US10406133B2 (en) 2013-03-14 2019-09-10 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US9090558B2 (en) 2013-03-14 2015-07-28 Alkermes Pharma Ireland Limited Prodrugs of fumarates and their use in treating various diseases
US9604922B2 (en) 2014-02-24 2017-03-28 Alkermes Pharma Ireland Limited Sulfonamide and sulfinamide prodrugs of fumarates and their use in treating various diseases
US9820960B2 (en) 2014-02-28 2017-11-21 Banner Life Sciences Llc Fumarate ester pharmaceutical compositions
US10918616B2 (en) 2014-02-28 2021-02-16 Banner Life Sciences Llc Fumarate ester pharmaceutical compositions
US9326965B2 (en) 2014-02-28 2016-05-03 Banner Life Sciences Llc Controlled release fumarate esters
US9326947B1 (en) 2014-02-28 2016-05-03 Banner Life Sciences Llc Controlled release fumarate esters
US10098863B2 (en) 2014-02-28 2018-10-16 Banner Life Sciences Llc Fumarate esters
US9511043B2 (en) 2014-02-28 2016-12-06 Banner Life Sciences Llc Fumarate ester pharmaceutical compositions
US10105337B2 (en) 2014-02-28 2018-10-23 Banner Life Sciences Llc Fumarate ester pharmaceutical compositions
US10105336B2 (en) 2014-02-28 2018-10-23 Banner Life Sciences Llc Fumarate ester pharmaceutical compositions
US9517209B2 (en) 2014-02-28 2016-12-13 Banner Life Sciences Llc Fumarate ester pharmaceutical compositions
US10918617B2 (en) 2014-02-28 2021-02-16 Banner Life Sciences Llc Fumarate ester pharmaceutical compositions
US10918615B2 (en) 2014-02-28 2021-02-16 Banner Life Sciences Llc Fumarate esters
US9814691B2 (en) 2014-02-28 2017-11-14 Banner Life Sciences Llc Fumarate ester pharmaceutical compositions
WO2016153957A3 (en) * 2015-03-20 2016-11-10 Biogen Ma Inc. Methods and compositions for the intravenous administration of fumarates for the treatment of neurological diseases
US9566259B1 (en) 2015-08-31 2017-02-14 Banner Life Sciences Llc Fumarate ester dosage forms
US9636318B2 (en) 2015-08-31 2017-05-02 Banner Life Sciences Llc Fumarate ester dosage forms
US10945985B2 (en) 2015-08-31 2021-03-16 Banner Life Sciences Llc Fumarate ester dosage forms
US9636319B1 (en) 2015-08-31 2017-05-02 Banner Life Sciences Llc Fumarate ester dosage forms
US10105335B2 (en) 2015-08-31 2018-10-23 Banner Life Sciences Llc Fumarate ester dosage forms
US11590095B2 (en) 2015-08-31 2023-02-28 Banner Life Sciences Llc Fumarate ester dosage forms
US9814692B2 (en) 2015-08-31 2017-11-14 Banner Life Sciences Llc Fumarate ester dosage forms
US9820961B2 (en) 2015-08-31 2017-11-21 Banner Life Sciences Llc Fumarate ester dosage forms
US11903918B2 (en) 2020-01-10 2024-02-20 Banner Life Sciences Llc Fumarate ester dosage forms with enhanced gastrointestinal tolerability

Also Published As

Publication number Publication date
HK1202885A1 (en) 2015-10-09
EP2791181A1 (en) 2014-10-22
US20140336151A1 (en) 2014-11-13
US9421273B2 (en) 2016-08-23
EP2791181A4 (en) 2015-08-05

Similar Documents

Publication Publication Date Title
US9421273B2 (en) Silicon-containing fumaric acid esters
AU2018260937B2 (en) Pharmaceutical compositions containing dimethyl fumarate
TW384288B (en) 5-substituted derivatives of mycophenolic acid
WO1996006068A1 (en) Benzene compound and medicinal use thereof
US9862721B2 (en) Tetrahydrocarboline derivative
CN102906090A (en) 1-(5-tert-butyl-2-phenyl-2h-pyrazol-3-yl)-3-[2-fluoro-4-(1-methyl-2-oxo-2,3-dihydro-1h-imidazo[4,5-b]pyridin-7-yloxy)-phenyl]-urea and related compounds and their use in therapy
TW200813055A (en) Spiro-conjugated cyclic group containing compound and use thereof
WO2015128492A1 (en) Monomethyl- and dimethylfumarate for nk cell activation
EP3419959A1 (en) Combination therapy
CN106660992B (en) Dimethylpyridine derivative and medical application thereof
CA2931064C (en) Withanolides useful for the treatment of neurodegenerative diseases
CA3079042A1 (en) D-amino acid oxidase inhibitors and therapeutic uses thereof
KR20000065102A (en) Inhibition of vascular cell adsorption molecule-1 using 2,6-di-alkyl-4-silyl-phenol and treatment of chronic inflammatory diseases
CN114656453B (en) Heptamethine indocyanine-TEMPO chemical even chain small molecule, preparation method and application thereof in preparation of radiation protection preparation
TWI537245B (en) Compounds for use in the treatment of autoimmune inflammatory disease
US20220259164A1 (en) Use of aminothiol compounds as cerebral nerve or heart protective agent
CN101081853A (en) 7-phenylpyrazolopyridine compounds
JPH0764727B2 (en) Anticonvulsant compounds and methods of use thereof
US9428431B2 (en) Compositions comprising CB receptor agonists, uses thereof and methods for their preparation
CN113387864B (en) S-indole benzamide derivative and preparation method and application thereof
JP7348214B2 (en) Crystal forms of HDAC6 selective inhibitors and uses thereof
JP2011509994A (en) Tetrahydrocyclopenta [c] acridine derivatives as kinase inhibitors and their biological applications
WO2017198159A1 (en) Imidazole derivative containing bridge ring
US20180215703A1 (en) Stearoyl amino acid salt and method of use thereof
JPH0680563A (en) Anti-hyperlipemia agent and anti-arteriosclerosis agent

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12856877

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 14363546

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

REEP Request for entry into the european phase

Ref document number: 2012856877

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2012856877

Country of ref document: EP