WO2013085540A3 - Cry crystals for the production of antimicrobial proteins - Google Patents

Cry crystals for the production of antimicrobial proteins Download PDF

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Publication number
WO2013085540A3
WO2013085540A3 PCT/US2011/064174 US2011064174W WO2013085540A3 WO 2013085540 A3 WO2013085540 A3 WO 2013085540A3 US 2011064174 W US2011064174 W US 2011064174W WO 2013085540 A3 WO2013085540 A3 WO 2013085540A3
Authority
WO
WIPO (PCT)
Prior art keywords
fusion polypeptides
cry
crystals
production
antimicrobial proteins
Prior art date
Application number
PCT/US2011/064174
Other languages
French (fr)
Other versions
WO2013085540A2 (en
Inventor
Michael K. Chan
Manoj S. Nair
Original Assignee
The Ohio State University Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Ohio State University Research Foundation filed Critical The Ohio State University Research Foundation
Priority to PCT/US2011/064174 priority Critical patent/WO2013085540A2/en
Publication of WO2013085540A2 publication Critical patent/WO2013085540A2/en
Publication of WO2013085540A3 publication Critical patent/WO2013085540A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Disclosed are fusion polypeptides comprising a Cry protein, or crystal-forming domain thereof, and an antimicrobial protein. Also provided are nucleic acid molecules encoding the fusion polypeptides, protein crystals comprising the fusion polypeptides, methods for producing and isolating the fusion polypeptides, methods for producing and isolating an antimicrobial polypeptides, compositions comprising the fusion polypeptides, and methods of using the fusion polypeptides.
PCT/US2011/064174 2011-12-09 2011-12-09 Cry crystals for the production of antimicrobial proteins WO2013085540A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/US2011/064174 WO2013085540A2 (en) 2011-12-09 2011-12-09 Cry crystals for the production of antimicrobial proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2011/064174 WO2013085540A2 (en) 2011-12-09 2011-12-09 Cry crystals for the production of antimicrobial proteins

Publications (2)

Publication Number Publication Date
WO2013085540A2 WO2013085540A2 (en) 2013-06-13
WO2013085540A3 true WO2013085540A3 (en) 2014-04-10

Family

ID=48575034

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/064174 WO2013085540A2 (en) 2011-12-09 2011-12-09 Cry crystals for the production of antimicrobial proteins

Country Status (1)

Country Link
WO (1) WO2013085540A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103484400A (en) * 2013-08-30 2014-01-01 昆明保腾生化技术有限公司 Engineering bacteria for killing pests, preventing diseases and promoting growth, and construction method and application thereof
CN103525837B (en) * 2013-09-18 2018-02-16 四川农业大学 Bt PROTEIN C ry72Aa1 operon genes and its application
US11807670B2 (en) * 2016-08-10 2023-11-07 The Chinese University Of Hong Kong Fusion proteins with improved properties
EP3956350A4 (en) * 2019-04-16 2023-05-10 The Chinese University Of Hong Kong Engineered cry proteins for delivery of therapeutics
EP3959321A4 (en) * 2019-04-26 2023-06-14 The Chinese University Of Hong Kong In vivo immobilization of proteins
FR3135094A1 (en) * 2022-05-02 2023-11-03 Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement Bacterial heterologous protein expression system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040018190A1 (en) * 2000-11-24 2004-01-29 Kunio Ando Drugs and foods improving the quality of life and process for producing the same
US20040166561A1 (en) * 2001-05-18 2004-08-26 Poulter Russell Tony Maskell Novel intein and uses thereof
US20050277181A1 (en) * 2003-12-23 2005-12-15 Berthet Francois X Compositions and methods for detecting pathogen infection
US20100322977A1 (en) * 2009-06-05 2010-12-23 The Ohio State University Research Foundation Biomaterials, compositions, and methods

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040018190A1 (en) * 2000-11-24 2004-01-29 Kunio Ando Drugs and foods improving the quality of life and process for producing the same
US20040166561A1 (en) * 2001-05-18 2004-08-26 Poulter Russell Tony Maskell Novel intein and uses thereof
US20050277181A1 (en) * 2003-12-23 2005-12-15 Berthet Francois X Compositions and methods for detecting pathogen infection
US20100322977A1 (en) * 2009-06-05 2010-12-23 The Ohio State University Research Foundation Biomaterials, compositions, and methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MATSUNAGA ET AL.: "Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins.", BMC BIOTECHNOL., vol. 10, no. 42, 4 June 2010 (2010-06-04), pages 1 - 9 *

Also Published As

Publication number Publication date
WO2013085540A2 (en) 2013-06-13

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