WO2013078519A1 - Kit et couvercle pour collecte d'éluat dans des procédés d'extraction en phase solide - Google Patents

Kit et couvercle pour collecte d'éluat dans des procédés d'extraction en phase solide Download PDF

Info

Publication number
WO2013078519A1
WO2013078519A1 PCT/BR2012/000252 BR2012000252W WO2013078519A1 WO 2013078519 A1 WO2013078519 A1 WO 2013078519A1 BR 2012000252 W BR2012000252 W BR 2012000252W WO 2013078519 A1 WO2013078519 A1 WO 2013078519A1
Authority
WO
WIPO (PCT)
Prior art keywords
eluate
vacuum
cap
solid phase
phase extraction
Prior art date
Application number
PCT/BR2012/000252
Other languages
English (en)
Portuguese (pt)
Inventor
Ricardo Mathias ORLANDO
Susanne Rath
Jarbas José Rodrigues ROHWEDDER
Original Assignee
Universidade Estadual De Campinas - Unicamp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidade Estadual De Campinas - Unicamp filed Critical Universidade Estadual De Campinas - Unicamp
Publication of WO2013078519A1 publication Critical patent/WO2013078519A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
    • B01L3/5635—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors connecting two containers face to face, e.g. comprising a filter
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques
    • G01N2001/4061—Solvent extraction

Definitions

  • This patent application relates to an adapted lid-type device for solid phase extraction procedures that allows the collection of the eluate directly into a collecting container without the need to introduce this container into the vacuum chamber of the containers.
  • manifold systems This device has a cartridge or extraction disk engagement region in addition to a pipe-coupled channel that in turn connects to the manifold system valves to transfer the vacuum force from these systems into the manifold. It also refers to an Eluate Collection Kit consisting of the adapted cap, a container and a hose to direct the vacuum to the cap.
  • the object of the present invention is intended for solid phase extraction systems and has industrial application mainly directed to laboratories and industries in the chemical, pharmaceutical, food and biotechnology areas.
  • Solid phase extraction with cartridges and discs represents a large portion of the sample preparation procedures due to the peculiar advantages of this technique (Spears, 2004).
  • Standard SPE procedures require four to five steps in which the liquid sample and other solvents pass through the sorbent that fills the inside of the cartridge or disc.
  • the device described in this patent overcomes these limitations and also allows a much larger variety of collecting containers to be used.
  • the caps described herein may be coupled to any commercial manifold system intended for conventional cartridges and disks using vacuum.
  • the present patent application refers to an adapted lid type device for collecting eluate for use together with manifold devices for solid phase extraction employing extraction cartridges and discs or any vacuum or reduced pressure device (below atmospheric pressure).
  • the lid consists basically of:
  • a channel which is inserted, preferably in an upright position, into the cap and which serves to receive a pipe or valve.
  • a pipe or valve with a place for the cartridge or extraction disc to be inserted, which preferably inserts itself into the cap and which serves to guide the eluate to the container or collecting tube.
  • a second channel through the cap with one end connected to a vacuum line.
  • Vacuum tubing with a fitting for the second channel of the lid and another fitting similar to the nozzles or lower terminals of cartridge and extraction discs to fit conventional manifold valves.
  • kit for obtaining the eluate consisting of:
  • the present invention further comprises the use of the cap described for any solid phase extraction process whether or not coupled to a vacuum pump and / or a peristaltic pump.
  • the main advantage over the state of the art is that it will not be necessary to wait for all cartridges to complete the washing or drying step for the eluate to be collected since
  • the tubes are not placed inside the vacuum chamber.
  • the vial or collecting tube also does not come into contact with the vacuum chamber contaminated with the sample spray formed during the predecessor steps.
  • Figure 1 shows a schematic representation of a conventional commercial manifold SPE system with cartridges (1), manifolds (2) and cap (3) with valves (4).
  • Figure 2 depicts a schematic representation of the prototype of the invention in exploded view of the developed invention called a "cap adapter” with the cartridge insert (5), eluate guide tube (6), adapter base (7), vacuum tube coupling region (8), eluate manifold bottle cap (10) and sealing rings (9).
  • Figure 3 is a schematic representation of the prototype of the invention in bottom, top and front views, respectively, of the constructive model called "cap adapter” with emphasis on the vacuum channel (11) that connects to the pipe coupling region. in vacuum transfer (8) and other detached portions (5, 6, 7, 8, 9, 10) already described in figure 2.
  • Figure 4 shows a schematic representation of the prototype of the invention in perspective view of the constructive model called "cap adapter” coupled to a manifold (12) and also to a vacuum transfer tubing (13) with an end coupled to the region. for coupling the vacuum tube (8) and the other to the vacuum line coupling adapter (14).
  • the other highlighted parts (5, 6, 7, 9 and 10) are described in figure 2.
  • Figure 5 shows a schematic representation of the prototype of the invention in perspective, lower, front and upper perspective view of the constructional model called "threaded fitting cap” with emphasis on the region of the thread (16) which is inserted and is part of the base of the lid (15) and with the other detached constructive features (5, 6, 8, 10) similar to those described in figures 2 and 3, but which are part or are constructed in the lid itself.
  • Figure 6 shows a schematic representation of the prototype of the invention in perspective and front view of the constructive model called "tapered and adapted portion lid” with emphasis on the conical region (18) which is inserted and forms part of the base of the lid (17). ) and serves to fit the manifolds.
  • the other highlighted design features (5 and 8) are similar to those described in Figure 5 and are also part of or constructed on the lid itself.
  • the present patent application relates to a lid-type device adapted with region for eluate collection vials, place for fitting cartridges and solid phase extraction discs, one for directing eluate and another for transferring vacuum to inside the collection bottle.
  • Said device sometimes referred to as a "cap adapter" consists basically of a cartridge fitting part (5), an eluate conduction tube (6), a vacuum channel (11), a base of the adapter (7), a vacuum tube coupling region (8), a bore cap (10) for the eluate collection bottle and sealing rings (9).
  • the cartridge or extraction disk is positioned in the upper portion of the lid by means of the cartridge insert (5).
  • the cartridge eluate is directed into the collecting bottle by an eluate conduction tube (6) through the action of gravity and / or with the aid of vacuum.
  • the vacuum or less than atmospheric pressure produced by any suitable device is transferred into the collecting vial by means of a vacuum channel (11) which connects to the coupling region of the vacuum transfer tubing (8) and is , in turn, connects with the vacuum transfer tubing (13).
  • Vacuum transfer tubing (13) can transfer vacuum from a conventional solid phase extraction manifold via a vacuum line coupling adapter (14) or be coupled to any vacuum system or peristaltic pump by middle of its end.
  • the operator does not have to wait for all cartridges to complete the washing or drying step for the eluate to be collected, since the tubes are not placed inside the vacuum chamber but are placed on shelves. or conventional tube and vial holder holders.
  • the vial or collecting tube also does not come into contact with the vacuum chamber contaminated with the sample spray formed during the predecessor steps.
  • the final purified eluate does not have to travel the same path through which the sample has passed which avoids cross contamination.
  • cap adapter screw cap fitting
  • conical cap fitting a constructive possibility that the caps are fitted to the eluate collection bottles. Except for the way the caps are fitted to the eluate collection bottles all three modes have the same mode of operation and functionality.
  • cap adapter you can adapt a wide variety of collection bottle cap types without the need to purchase a new cap for each bottle you need.
  • the original collection bottle caps are employed which receive an adapter in a hole in the upper portion of the cap.
  • the "Screw-In Tailored Cap” construction model allows the user to use a screw-in ready-made cap that is specific to a particular bottle configuration, which facilitates use and requires no adjustments.
  • the "tailored and conical portion" construction model allows the user to also use a ready-made cap, but with a snap fit that fits a wider range of vials by introducing the conical region into the vial mouth. .
  • Cartridges or discs that may be employed in the solid phase extraction kit of the invention are all those used in conventional manifold systems.
  • the present patent application further comprises the use of the device (and also the kit) described for any solid phase extraction process, whether or not coupled to a vacuum pump, and / or a peristaltic pump and / or a solid phase extraction manifold.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Closures For Containers (AREA)

Abstract

La présente demande de brevet d'invention concerne un dispositif de type couvercle adapté et un kit pour la collecte d'un éluat utilisé dans des procédés d'extraction en phase solide conjointement avec des systèmes de type "collecteur" ou des systèmes basse pression en général. Ce système de couvercles permet de collecter l'éluat dans des tubes collecteurs de tailles variables, voire même de taille supérieure à celle de la chambre à vide des "collecteurs", sans nécessité de l'introduire dans cette chambre à vide, ou de façon que le nombre d'arrêts et la contamination pendant le procédé soient réduits.
PCT/BR2012/000252 2011-11-30 2012-07-23 Kit et couvercle pour collecte d'éluat dans des procédés d'extraction en phase solide WO2013078519A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRPI1105218-0A BRPI1105218B1 (pt) 2011-11-30 2011-11-30 Kit e tampa para coleta do eluato em procedimentos de extração em fase sólida
BRPI1105218-0 2011-11-30

Publications (1)

Publication Number Publication Date
WO2013078519A1 true WO2013078519A1 (fr) 2013-06-06

Family

ID=48534543

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2012/000252 WO2013078519A1 (fr) 2011-11-30 2012-07-23 Kit et couvercle pour collecte d'éluat dans des procédés d'extraction en phase solide

Country Status (2)

Country Link
BR (1) BRPI1105218B1 (fr)
WO (1) WO2013078519A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4810471A (en) * 1986-07-24 1989-03-07 Rohm And Haas Co. Vacuum manifold for extraction processing
US5849249A (en) * 1997-03-17 1998-12-15 Whatman Inc. Solid phase extraction apparatus for enhanced recovery and precision

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4810471A (en) * 1986-07-24 1989-03-07 Rohm And Haas Co. Vacuum manifold for extraction processing
US5849249A (en) * 1997-03-17 1998-12-15 Whatman Inc. Solid phase extraction apparatus for enhanced recovery and precision

Also Published As

Publication number Publication date
BRPI1105218A2 (pt) 2015-12-15
BRPI1105218B1 (pt) 2020-09-15

Similar Documents

Publication Publication Date Title
US20210322980A1 (en) Whole-process biological detection device
CN111132724B (zh) 真空液体提取和纯化系统及方法
EP3244188B1 (fr) Utilisation d'un dispositif d'échantillonnage
US20110104026A1 (en) Apparatus for extracting biochemical materials from biological samples
WO2012109717A1 (fr) Dispositif de type "collecteur" pour extraction en phase solide avec chambres et valves individuelles pour la régulation d'un éluat par pompe péristaltique et/ou par vide total
JPS63171337A (ja) 抽出処理用真空マニホルド
WO2005105977A1 (fr) Couvercle destine aux bouteilles d'un bioreacteur et/ou aux tubes de centrifugation
CN102634448A (zh) 一种生物采样拭子
CN206463518U (zh) 一种便于收集溶剂的旋转蒸发仪
WO2013078519A1 (fr) Kit et couvercle pour collecte d'éluat dans des procédés d'extraction en phase solide
BR112018008386B1 (pt) Dispositivo e conjunto de preparação de amostra
CN104028006B (zh) 层析装置
US7534397B2 (en) Sample preparation device
CN113477415A (zh) 一种检验科血液离心分层装置
CN204101334U (zh) 一种蒸汽取样装置
BRMU9101903Y1 (pt) dispositivo para extração em fase sólida tipo "manifold" com câmara de vácuo, válvulas para controle por vácuo e válvulas adaptadas para controle por bomba peristáltica
CN212142662U (zh) 一种用于回收西林瓶中样品的实验连接装置
BRMU8903082Y1 (pt) Device with valves and routes for control of solid phase extraction system manifold
CN208420483U (zh) 一种新型固相萃取仪
CN106834422A (zh) 面向安瓿瓶装水溶液供试品的全自动化流水线集菌操作方法和系统
CN209438625U (zh) 一种改进型96孔板
CN207899467U (zh) 一种医药技术开发研究用试剂存放装置
CN205861441U (zh) 药品无菌检查富集用并联式过滤器
CN216129571U (zh) 一种核酸提取装置
CN102841148A (zh) 固相萃取柱转接套管

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12852703

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 12852703

Country of ref document: EP

Kind code of ref document: A1