WO2013050867A3 - Chitinase issue de brevibacillus laterosporus, sa production et son utilisation - Google Patents

Chitinase issue de brevibacillus laterosporus, sa production et son utilisation Download PDF

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Publication number
WO2013050867A3
WO2013050867A3 PCT/IB2012/001982 IB2012001982W WO2013050867A3 WO 2013050867 A3 WO2013050867 A3 WO 2013050867A3 IB 2012001982 W IB2012001982 W IB 2012001982W WO 2013050867 A3 WO2013050867 A3 WO 2013050867A3
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WO
WIPO (PCT)
Prior art keywords
chitinase
new
insecticidal
lak
agriculture
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PCT/IB2012/001982
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English (en)
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WO2013050867A2 (fr
WO2013050867A4 (fr
Inventor
Lakshmi Prasanna GANGAVARAMU
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Gangavaramu Lakshmi Prasanna
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Application filed by Gangavaramu Lakshmi Prasanna filed Critical Gangavaramu Lakshmi Prasanna
Publication of WO2013050867A2 publication Critical patent/WO2013050867A2/fr
Publication of WO2013050867A3 publication Critical patent/WO2013050867A3/fr
Publication of WO2013050867A4 publication Critical patent/WO2013050867A4/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne une souche inédite de Brevibacillus laterosporus LAK 1210 (MTCC 5487), dont le numéro d'enregistrement est MTCC 5487, et qui peut être utilisée en vue de la production combinée de protéines insecticides et d'enzymes chitinolytiques inductibles ayant une utilité potentielle dans la lutte contre les insectes nuisibles tant en agriculture qu'en sylviculture, dans la lutte contre les maladies des plantes et dans les programmes de lutte contre les moustiques. Grâce à ses effets multiples, puisqu'elle peut également être utilisée en vue de la production de chitinase ayant la capacité de dégrader les déchets marins, cette souche insecticide inédite de Brevibacillus laterosporus LAK 1210 a de nombreuses applications biotechnologiques dans le domaine de l'agriculture, de la sylviculture, de la médecine et de l'environnement. L'invention concerne, en outre, une nouvelle chitinase dotée de caractéristiques inédites, qui est issue d'une source toute récente de microorganismes, et ses applications inédites dans le cadre de l'utilisation de la biomasse et de la gestion des déchets. Les procédés et compositions de l'invention font appel à des cellules végétatives, à leurs dérivés (spores, extraits cellulaires, fractions enzymatiques) et à des polypeptides pour la mise au point d'un pesticide naturel permettant de lutter contre les insectes et les champignons phytopathogènes. Un autre objet de l'invention consiste à étendre l'effet synergique des chitinases en matière d'activité insecticide pour une activité pesticide améliorée impactant un plus large éventail d'animaux nuisibles.
PCT/IB2012/001982 2011-10-08 2012-10-08 Chitinase issue de brevibacillus laterosporus, sa production et son utilisation WO2013050867A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161545120P 2011-10-08 2011-10-08
US61/545,120 2011-10-08

Publications (3)

Publication Number Publication Date
WO2013050867A2 WO2013050867A2 (fr) 2013-04-11
WO2013050867A3 true WO2013050867A3 (fr) 2013-10-24
WO2013050867A4 WO2013050867A4 (fr) 2013-12-19

Family

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Family Applications (1)

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PCT/IB2012/001982 WO2013050867A2 (fr) 2011-10-08 2012-10-08 Chitinase issue de brevibacillus laterosporus, sa production et son utilisation

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Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10004236B2 (en) * 2012-09-24 2018-06-26 Lincoln University Biocontrol compositions
BR112016002766A8 (pt) * 2013-08-12 2020-02-04 Bio Cat Microbials Llc composições compreendendo cepas de bacillus e métodos para a preparação das referidas composições e para suprimir as atividades e o crescimento de fungos patogênicos de plantas
CN104480046B (zh) * 2014-12-18 2017-04-05 江苏省农业科学院 一株侧孢短芽孢杆菌及其应用
CA3168036A1 (fr) 2017-03-27 2018-10-04 Tenfold Technologies, LLC Procedes et compositions agricoles permettant de prevenir ou de lutter contre les maladies des plantes
CN107151641B (zh) * 2017-07-04 2020-11-27 沈阳农业大学 一株抑制水稻纹枯病菌的侧孢短芽孢杆菌及其应用
US11168314B2 (en) 2017-09-05 2021-11-09 Department Of Biotechnology Modified chitinase from Brevibacillus laterosporus LAK 1210 and uses thereof
CN110699276A (zh) * 2019-09-30 2020-01-17 广西民族大学 一种嗜几丁质类芽孢杆菌的菌株及其应用
CN112779178B (zh) * 2019-11-08 2023-02-17 财团法人农业科技研究院 地衣芽孢杆菌a6菌株及其应用
CN111454848B (zh) * 2020-04-15 2022-04-19 中国科学院微生物研究所 一种雅致放射毛霉gd48及其发酵产物、菌剂与应用
CN114480197B (zh) * 2022-02-09 2022-10-04 山东农业大学 一株侧孢短芽孢杆菌及菌剂与其在生物药肥中的应用
CN114456987B (zh) * 2022-03-11 2023-11-24 天津科技大学 一种侧孢短芽孢杆菌、芽孢抗菌肽-壳聚糖-明胶复合保鲜膜的制备方法和应用
CN115364123B (zh) * 2022-07-05 2023-08-25 江苏三仪生物工程有限公司 一种侧孢短芽孢杆菌及其在反刍寄生虫防治中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5045314A (en) * 1989-11-14 1991-09-03 The United States Of America As Represented By The Secretary Of Agriculture Control of parasitic nematode ova/larvae with a bacillus laterosporus
WO2008031887A2 (fr) * 2006-09-15 2008-03-20 Universita' Degli Studi Di Sassari Souche de brevibacillus laterosporus, composition contenant cette souche et procédé de contrôle biologique de diptères

Family Cites Families (2)

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Publication number Priority date Publication date Assignee Title
US3911110A (en) 1972-12-22 1975-10-07 Canadian Patents Dev Insecticidal compositions
US6121436A (en) 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
US5045314A (en) * 1989-11-14 1991-09-03 The United States Of America As Represented By The Secretary Of Agriculture Control of parasitic nematode ova/larvae with a bacillus laterosporus
WO2008031887A2 (fr) * 2006-09-15 2008-03-20 Universita' Degli Studi Di Sassari Souche de brevibacillus laterosporus, composition contenant cette souche et procédé de contrôle biologique de diptères

Non-Patent Citations (5)

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Title
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LAKSHMI PRASANNA ET AL: "A novel strain of Brevibacillus laterosporus produces chitinases that contribute to its biocontrol potential", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 97, no. 4, 29 April 2012 (2012-04-29), pages 1601 - 1611, XP055069226, ISSN: 0175-7598, DOI: 10.1007/s00253-012-4019-y *
M. LIU ET AL: "Chitinolytic activities in Bacillus thuringiensis and their synergistic effects on larvicidal activity", JOURNAL OF APPLIED MICROBIOLOGY, vol. 93, no. 3, 1 September 2002 (2002-09-01), pages 374 - 379, XP055069213, ISSN: 1364-5072, DOI: 10.1046/j.1365-2672.2002.01693.x *
SAIKIA R ET AL: "strain BPM3, a potential biocontrol agent isolated from a natural hot water spring of Assam, India", MICROBIOLOGICAL RESEARCH, FISCHER, JENA, DE, vol. 166, no. 3, 1 March 2010 (2010-03-01), pages 216 - 225, XP028153956, ISSN: 0944-5013, [retrieved on 20100406], DOI: 10.1016/J.MICRES.2010.03.002 *
SHANMUGAIAH V ET AL: "Optimization of cultural conditions for production of chitinase by Bacillus laterosporous MML2270 isolated from rice rhizosphere soil", AFRICAN JOURNAL OF BIOTECHNOLOGY, vol. 7, no. 15, August 2008 (2008-08-01), pages 2562 - 2568, XP002699911, ISSN: 1684-5315 *

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WO2013050867A2 (fr) 2013-04-11
WO2013050867A4 (fr) 2013-12-19

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