WO2013050760A3 - Cofactor regeneration system - Google Patents

Cofactor regeneration system Download PDF

Info

Publication number
WO2013050760A3
WO2013050760A3 PCT/GB2012/052451 GB2012052451W WO2013050760A3 WO 2013050760 A3 WO2013050760 A3 WO 2013050760A3 GB 2012052451 W GB2012052451 W GB 2012052451W WO 2013050760 A3 WO2013050760 A3 WO 2013050760A3
Authority
WO
WIPO (PCT)
Prior art keywords
electron transfer
cofactor regeneration
components
regeneration system
oxido
Prior art date
Application number
PCT/GB2012/052451
Other languages
English (en)
French (fr)
Other versions
WO2013050760A2 (en
Inventor
Kylie Vincent
Lars LAUTERBACH
Oliver Lenz
Original Assignee
Isis Innovation Limited
Humboldt-Universität Zu Berlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Innovation Limited, Humboldt-Universität Zu Berlin filed Critical Isis Innovation Limited
Priority to US14/349,126 priority Critical patent/US10801052B2/en
Priority to EP12772384.9A priority patent/EP2764107B1/en
Publication of WO2013050760A2 publication Critical patent/WO2013050760A2/en
Publication of WO2013050760A3 publication Critical patent/WO2013050760A3/en
Priority to US15/899,253 priority patent/US10975408B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/36—Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/99—Oxidoreductases acting on NADH or NADPH (1.6) with other acceptors (1.6.99)
    • C12Y106/99001—NADPH dehydrogenase (1.6.99.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/01—Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/01—Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
    • C12Y108/01004—Dihydrolipoyl dehydrogenase (1.8.1.4), i.e. lipoamide-dehydrogenase
PCT/GB2012/052451 2011-10-03 2012-10-03 Cofactor regeneration system WO2013050760A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US14/349,126 US10801052B2 (en) 2011-10-03 2012-10-03 Cofactor regeneration system
EP12772384.9A EP2764107B1 (en) 2011-10-03 2012-10-03 Cofactor regeneration system
US15/899,253 US10975408B2 (en) 2011-10-03 2018-02-19 Cofactor regeneration system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1116971.1A GB201116971D0 (en) 2011-10-03 2011-10-03 Cofactor regeneration system
GB1116971.1 2011-10-03

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US14/349,126 A-371-Of-International US10801052B2 (en) 2011-10-03 2012-10-03 Cofactor regeneration system
US15/899,253 Continuation US10975408B2 (en) 2011-10-03 2018-02-19 Cofactor regeneration system

Publications (2)

Publication Number Publication Date
WO2013050760A2 WO2013050760A2 (en) 2013-04-11
WO2013050760A3 true WO2013050760A3 (en) 2013-06-13

Family

ID=45035019

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2012/052451 WO2013050760A2 (en) 2011-10-03 2012-10-03 Cofactor regeneration system

Country Status (4)

Country Link
US (2) US10801052B2 (enIt)
EP (1) EP2764107B1 (enIt)
GB (1) GB201116971D0 (enIt)
WO (1) WO2013050760A2 (enIt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017011870A1 (en) * 2015-07-20 2017-01-26 Commonwealth Scientific And Industrial Research Organisation Molecular machines
CN107849589A (zh) * 2015-07-24 2018-03-27 安尼基有限责任公司 用于酶促制备混合糖的氧化产物和还原产物的方法
CN109666689B (zh) * 2017-10-15 2022-11-15 中国科学院天津工业生物技术研究所 一种重组高温镍铁氢化酶的异源表达纯化方法及其应用
EP3524684A1 (en) * 2018-02-12 2019-08-14 Oxford University Innovation Ltd. Method for producing a deuterated or tritiated nad(p)h
KR20210043606A (ko) * 2018-08-10 2021-04-21 메타볼릭 익스플로러 1,3-프로판디올 및 부티르산 생산이 개선된 미생물
GB202006047D0 (en) * 2020-04-24 2020-06-10 Univ Oxford Innovation Ltd Method
US20240102177A1 (en) * 2021-02-04 2024-03-28 King Adbullah University Of Science And Technology Electrochemical cofactor regeneration using earth abundant electrodes for biocatalytic applications
GB202206982D0 (en) * 2022-05-12 2022-06-29 Univ Oxford Innovation Ltd Reduction method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000053731A2 (en) * 1999-03-11 2000-09-14 Eastman Chemical Company Enzymatic reductions with dihydrogen via metal catalyzed cofactor regeneration

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5538867A (en) 1988-09-13 1996-07-23 Elf Aquitaine Process for the electrochemical regeneration of pyridine cofactors
WO2006084276A2 (en) 2005-02-04 2006-08-10 The Regents Of The University Of California Enzyme catalyzed metallic nanoparticle synthesis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000053731A2 (en) * 1999-03-11 2000-09-14 Eastman Chemical Company Enzymatic reductions with dihydrogen via metal catalyzed cofactor regeneration

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FABIAN RUNDBCK ET AL: "Coupling of permeabilized cells ofandfor asymmetric ketone reduction using Has reductant", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 157, no. 1, 5 October 2011 (2011-10-05), pages 154 - 158, XP028395742, ISSN: 0168-1656, [retrieved on 20111005], DOI: 10.1016/J.JBIOTEC.2011.09.029 *
KASHIWAGI Y ET AL: "Preparative, electroenzymatic reduction of ketones on an all components-immobilized graphite felt electrode", ELECTROCHIMICA ACTA, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 42, no. 13, 1 January 1997 (1997-01-01), pages 2267 - 2270, XP004061824, ISSN: 0013-4686, DOI: 10.1016/S0013-4686(97)85509-0 *
KOHLMANN ET AL: "Electroenzymatic synthesis", JOURNAL OF MOLECULAR CATALYSIS. B, ENZYMATIC, ELSEVIER, AMSTERDAM, NL, vol. 51, no. 3-4, 31 January 2008 (2008-01-31), pages 57 - 72, XP022442768, ISSN: 1381-1177, DOI: 10.1016/J.MOLCATB.2007.10.001 *
WICHMANN R ET AL: "Cofactor regeneration at the lab scale", ADVANCES IN BIOCHEMICAL ENGINEERING, BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 92, 1 January 2005 (2005-01-01), pages 225 - 260, XP009158439, ISSN: 0724-6145, DOI: 10.1007/B98911 *

Also Published As

Publication number Publication date
US10975408B2 (en) 2021-04-13
US10801052B2 (en) 2020-10-13
EP2764107B1 (en) 2018-12-05
GB201116971D0 (en) 2011-11-16
US20150044723A1 (en) 2015-02-12
EP2764107A2 (en) 2014-08-13
WO2013050760A2 (en) 2013-04-11
US20180298415A1 (en) 2018-10-18

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